Reproduction and breeding最新文献

{"title":"Fabrication and characterization of single-wall carbon nanotube and its biocompatibility to human hepatocytes","authors":"Hatef Rahim Sabbaghizadeh ,&nbsp;Arshin Oskoueian ,&nbsp;Amir Hossein Ashtari","doi":"10.1016/j.repbre.2024.12.001","DOIUrl":"10.1016/j.repbre.2024.12.001","url":null,"abstract":"<div><div>Single-wall carbon nanotubes (SWCNTs) have emerged as promising nanocarriers for targeted cancer drug delivery due to their unique structural properties. However, their cytotoxicity remains a significant challenge, as the biocompatibility of SWCNTs with human cells, particularly hepatocytes, is crucial for their clinical application. The toxicity of SWCNTs is influenced by factors such as nanoparticle size, morphology, surface chemistry, and the presence of impurities. In this study, we aimed to synthesize highly pure SWCNTs and assess their biocompatibility with human hepatocyte cells.</div><div>SWCNTs were fabricated using a modified chemical vapor deposition (CVD) method, followed by a two-step acid purification technique. Raman spectroscopy and electron microscopy confirmed a high purity level of 99.8 %. The biocompatibility of the purified SWCNTs was evaluated using an in vitro model with human hepatocytes. Results indicated that high concentrations of SWCNTs (&gt;50 μg/ml) significantly reduced cell viability, increased lactate dehydrogenase leakage, and elevated lipid peroxidation, while simultaneously suppressing antioxidant enzyme activity.</div><div>Flow cytometry analysis further revealed that exposure to high concentrations of SWCNTs induced apoptosis in hepatocytes. Molecular analysis of key biomarkers demonstrated upregulation of TNF-α, IL1β, NF-kB, and iNOS, alongside downregulation of nrf2 gene and protein expression. These alterations contribute to the mechanisms underlying SWCNT-induced oxidative stress and apoptosis in human hepatocyte cells. Despite the high purity of SWCNTs, their cytotoxic effects may be attributed to their inherent physical properties, including rigidity, surface area, and fiber length.</div><div>In conclusion, while SWCNTs hold great potential for cancer drug delivery, managing their toxicity remains critical for their future therapeutic applications.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 29-37"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widening the tool set for breeding superior banana cultivars. Can new techniques of pollen handling and pollination help overcome the lack of recombinant seed in banana breeding?","authors":"Ralf Bodo Trognitz","doi":"10.1016/j.repbre.2024.11.001","DOIUrl":"10.1016/j.repbre.2024.11.001","url":null,"abstract":"<div><div>Pollen tube growth (PTG) monitoring was applied to florets of domesticated bananas and plantains. Observations were made in UV microscopy after Aniline Blue staining specific for callose, a carbohydrate of pollen tubes. It was found that total pollen grain numbers on a stigma and total pollen tubes developing after sufficient time for growing to reach the ovules within a female floret ranged from nil to few. This suggested the standard hand pollination technique, HP, may be insufficient to achieve desired pollination quality and respective fertilization for seed development. Pollen deposition and pollen tube growth was at least ten times larger when applying the newly developed pollen-anther-stigma, PAS, technique of pollination. This PAS technique includes mechanically working wilted anther pieces holding and exposing the pollen into the female stigma. PAS provided the potential for maximum seed formation depending on genetic limitations that may be present. PAS is therefore recommended for use in investigations of reproductive processes, such as sterility and self-incompatibility, and it can help increasing the production of recombinant seeds in planned crosses for breeding and selection.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 23-28"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of an integrated linkage map of sugarcane using unigene-derived microsatellite (SSR) markers","authors":"Sujeet P. Singh ,&nbsp;Jyoti Rasogi ,&nbsp;Chethana Ck ,&nbsp;R.K. Singh ,&nbsp;A. Nigam ,&nbsp;Ram Baran Singh","doi":"10.1016/j.repbre.2024.11.002","DOIUrl":"10.1016/j.repbre.2024.11.002","url":null,"abstract":"<div><div>Sugarcane (<em>Saccharum officinarum</em> L.) is one of the key agro-industrial crops that produce raw sugar and bio-fuel ethanol, which contributes to sustainable national economy, food and nutritional security in tropical areas globally. Traditional sugarcane breeding practices are tedious and labour-intensive practice to develop elite high-yielding and resistant cultivars. Sugarcane improvement based on high-throughput techniques to develop traits linked DNA markers through establishing marker-trait associations using linkage analysis. The SSR and markers offer a user-friendly and cost-efficient genomic tool for marker-trait linkage analysis in crop species. In the current study, an integrated sugarcane genetic linkage map was constructed with SSR and TRAP molecular markers using 226 progenies derived from a bi-parental (UP 9530 × Co 86,011) mapping population. The biparental mapping population (266 progenies) was genotyped which generated a total of 815 polymorphic loci with 96 SSR and 11 TRAP primer combinations. Out of 815 markers, 324 markers were scattered onto 107 linkage groups (LGs) and for all the linkage maps, the cumulative genome length was found to be 7608.7 cM, with an average length of 71.11 cM per LG. The length of LGs was ranging from 0.06 to 238.44 cM, with an average of 23.48 cM between two adjacent markers and the number of markers per LG varied from 2 to 11. Out of 324 linked markers, 232 (71.60 %) were found to be single dose (1:1) and 92 (28.39 %) double single dose (3:1) in segregation pattern in genotyping. All the LGs with common markers on individual maps were merged to construct an integrated map using a map integration tool. Future research will be focused on validation of the linked markers in sugarcane involves a combination of phenotypic studies, molecular analysis, genetic mapping, and field trials. This ensures the marker is accurate, reliable, and applicable across different environments and genetic backgrounds. Moreover, the developed SSR-based linkage map will be a useful suit for understanding genetic architecture, marker-assisted breeding for improving yield and quality, identifying disease resistance genes, and accelerating genetic research in polyploid sugarcane and other related polyploidy crop species.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 12-22"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and cytogenetic analysis of Moringa oleifera short and long capsule phenotypes","authors":"Martha Gómez-Martínez ,&nbsp;Jesús David García-Ortiz ,&nbsp;Susana Gómez-Martínez ,&nbsp;Adriana C. Flores-Gallegos ,&nbsp;M. Humberto Reyes-Valdés ,&nbsp;Jesús A. Morlett-Chávez ,&nbsp;Raúl Rodríguez-Herrera","doi":"10.1016/j.repbre.2024.10.002","DOIUrl":"10.1016/j.repbre.2024.10.002","url":null,"abstract":"<div><div>Cytogenetic and molecular studies hold significant importance in plant breeding programs. In the case of moringa, such studies are scarce. Therefore, the objective of this research was to determine the chromosomal number and genetic diversity parameters in two phenotypes of <em>Moringa oleifera Lam</em>. (short and long capsule) using chromosomal analysis and ISSR markers, respectively. Cytogenetic analyses were conducted using the “Somatic chromosomes in root apices” technique, with acetocarmine and papain for staining. It was possible to identify 2N = 28 chromosomes in a single cell of the long capsule phenotype, ranging in size from 0.05 to 0.10 μm. Cells in both moringa phenotypes were observed in prophase, metaphase, anaphase, and telophase. High genetic variability was found in both phenotypes of moringa, as indicated by a Shannon index of 0.81. Additionally, the principal component analysis and UPGMA (Unweighted Pair-Group Method with Arithmetic) groups revealed genetic isolation between the studied moringa phenotypes. The significant polymorphism obtained with most primers suggests that the tested ISSR markers are highly useful for studying genetic diversity in moringa. These findings provide a robust foundation for future research in genetic improvement and conservation of moringa, highlighting the relevance of cytogenetic and molecular studies in this species.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 5-11"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Masculinization? Rare variation in canine length among female water deer (Hydropotes inermis) can lead to errors in sex identification","authors":"Seong-Min Lee","doi":"10.1016/j.repbre.2024.10.001","DOIUrl":"10.1016/j.repbre.2024.10.001","url":null,"abstract":"<div><div>Most cervids feature antlers for mate competition, but water deer possess uniquely elongated maxillary canines instead. This study examined the presence of elongated canines, a trait usually seen in males, in female water deer from 185 individuals culled in South Korea. Measurements of body length, height, and mass were recorded, along with assessments of reproductive capability. The results showed that 1.6 % of the females exhibited elongated canines, retaining their fertility. Their body sizes remained within the typical range for their same age group, with their canines continuing to grow for at least three years. Thus, this rare variation, which is similar to males, may lead to infrequent errors in sex identification based on physical appearance.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 1-4"},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen characteristics, freezability, and application of motility-based protein markers (proAKAP4) in assessing the suitability of superior Bali bulls (Bos sondaicus) at the Regional AI Center","authors":"Kudratullah ,&nbsp;Raden Iis Arifiantini ,&nbsp;Enny Yuliani ,&nbsp;Berlin Pandapotan Pardede ,&nbsp;Syahruddin Said ,&nbsp;Bambang Purwantara","doi":"10.1016/j.repbre.2024.09.005","DOIUrl":"10.1016/j.repbre.2024.09.005","url":null,"abstract":"<div><div>The use of molecular markers in selecting superior bulls, including Bali bulls, for the AI program will significantly increase reproductive efficiency and affect the economic aspects of several countries, especially in the livestock industry sector. Motility-based protein markers, proAKAP4, as one of the molecular markers that have recently been developed in the breeding industry, are expected to be applied and used at AI centers in Indonesia to optimize the use of superior Bali bulls, which are also supported by various semen and freezability characteristics. Using all Bali bulls from the Regional AI center in one of the provinces in Indonesia, this study divided the bulls into motility grade A and motility grade B groups. The collected semen is tested using various methods based on the literature, including fresh and frozen semen and sperm freezability. The proAKAP4 was quantified using the Bull 4MID® Kit with ELISA. The characteristics of fresh semen were significantly different (P &lt; 0.05) except for sperm abnormalities (P &gt; 0.05). The frozen semen characteristics were significantly different (P &lt; 0.05) regarding plasma membrane integrity, DNA fragmentation, and several kinematic parameters. There was no significant difference (P &gt; 0.05) in the freezability characteristics of Bali bull sperm. ProAKAP4 was significantly (P &lt; 0.05) more abundant in the motility grade B bull group and was closely correlated (P &lt; 0.05) with progressive motility and sperm viability. Motility-based protein markers, proAKAP4, can be applied to bulls at the Regional AI Center, with quantification results showing that half of the bull population used in research does not meet the fitness standards for superior bulls.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 279-286"},"PeriodicalIF":0.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on stress resistance breeding in fish","authors":"Jie Hu ,&nbsp;Jie Yang ,&nbsp;Huan Liao","doi":"10.1016/j.repbre.2024.09.004","DOIUrl":"10.1016/j.repbre.2024.09.004","url":null,"abstract":"<div><div>With the continuing development of the aquaculture industry, the stress associated with many environmental factors has severely restricted the growth of fish, reduced fish resistance to disease, and had a large negative impact on fishery production. Therefore, it is of great importance for the sustainable development of aquaculture to cultivate new fish varieties with strong stress resistance. Traditional hybridization and gynogenesis, selective breeding, modern breeding techniques such as molecular marker-assisted breeding and whole genome selection breeding can be used to develop efficient and accurate fish breeding strategies, and to cultivate new fish varieties with high stress resistance. This paper reviews the status of research on fish breeding for disease resistance, cold resistance, high temperature tolerance, hypoxia tolerance, salt tolerance, saline-alkali tolerance as well as non-specific stress resistance traits, analyzes trends in research on fish breeding for stress tolerance, and explores future development directions. Furthermore, this paper proposes new ideas for fish seed industry innovation to promote its continuous and sustainable development.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 267-278"},"PeriodicalIF":0.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovation of carp germplasm and its research progress","authors":"Zhipeng Yang ,&nbsp;Qinglin Xu ,&nbsp;Xin Wang ,&nbsp;Huan Liu ,&nbsp;Yalan Zhang ,&nbsp;Wei Chen ,&nbsp;Sijin Fan ,&nbsp;Junfeng Wu ,&nbsp;Yaohui Li ,&nbsp;Huan Zhong ,&nbsp;Fangzhou Hu ,&nbsp;Ming Wen ,&nbsp;Kaikun Luo ,&nbsp;Shi Wang","doi":"10.1016/j.repbre.2024.09.003","DOIUrl":"10.1016/j.repbre.2024.09.003","url":null,"abstract":"<div><div>The artificial breeding of carp existed in China 8000 years ago. The breeding of carp played an important role in ancient Chinese history, and it still played a crucial role in modern aquaculture. The output of carp in China is very high, accounting for 8.42 % of the total output of freshwater products in 2023, ranking fifth in the total output of freshwater aquaculture fish. At the same time, carp is also the fish species with the highest number of new varieties bred in China. With the advent of the genomics era, breeding technologies have also made progress. This paper reviews the history and current status of carp farming, the germplasm resources of carp, and the application of fish genetic breeding technology in the germplasm innovation of carp.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 243-253"},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indigenous cattle biodiversity in India: Adaptation and conservation","authors":"Sujith Kumar Sarang,&nbsp;Damodaran Sreekumar,&nbsp;Veerasamy Sejian","doi":"10.1016/j.repbre.2024.09.001","DOIUrl":"10.1016/j.repbre.2024.09.001","url":null,"abstract":"<div><div>Indigenous breeds of cattle are suitable for varying climatic conditions of the tropical environment by virtue of a series of species-specific traits and adaptive strategies. Such traits and adaptive strategies could be generally classed into morphological/anatomical, physiological, behavioural, neuro-endocrine, haematological, and molecular and cellular features. At the same time, over the years it is found that the population of indigenous cattle is decreasing at a faster rate all over the world. This is mainly due to the preponderance of crosses of indigenous cattle with exotic/temperate high producing cattle for improved milk production. As a result, if suitable measures are not taken for conserving indigenous breeds of cattle, the diversity will go down drastically and may at one stage become highly endangered in status. For working out conservation plans and breeding strategies, the fundamental requirements include genetic characterization by means of gene and microsatellite markers along with evaluation of genetic variability, population structure and composition. Conserving the diversity of indigenous germplasm will help to choose breeds of cattle that adapt to changing climatic environment. Such an approach could help in formulating strategies for conservation and breeding plans and further could also lay the foundation for effective utilisation of indigenous cattle under different production systems.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 254-266"},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on sperm cryopreservation of hybrid fish derived from Carassius cuvieri (♀) × Carassius auratus red var (♂)","authors":"Qianqian Zeng ,&nbsp;Yixuan Chen ,&nbsp;Minyi Wang ,&nbsp;Yinggang Li ,&nbsp;Tao Dai ,&nbsp;Weiling Qin ,&nbsp;Yating Zhu ,&nbsp;Chun Zhang ,&nbsp;Yi Zhou ,&nbsp;Qinbo Qin ,&nbsp;Conghui Yang ,&nbsp;Qianhong Gu","doi":"10.1016/j.repbre.2024.09.002","DOIUrl":"10.1016/j.repbre.2024.09.002","url":null,"abstract":"<div><div>Cryopreservation of sperm is an effective method for conserving germplasm resources in fish genetic breeding. The high-quality hybrid fish (WR) derived from white crucian carp (<em>Carassius cuvieri</em>, WCC, ♀) and red crucian carp (<em>C</em>. <em>auratus</em> red var., RCC, ♂), possesses valuable traits such as high survival rates, strong resistance, and rapid growth, representing an important germplasm resources of crucian carp. This study compared the effects of different antifreeze solutions on sperm viability among three varieties (WR, WCC, and RCC) and examined changes in enzyme activity, fertilization rates, and hatching rates after cryopreservation, aiming to enhance the cryogenic sperm cryopreservation technique in hybrid fish and investigate the mechanisms underlying spermatozoa damage caused by cryopreservation. The results showed that the antifreeze combination of D14 with 15 % dimethyl sulfoxide (DMSO) had the best effect in preserving the sperm of WR and WCC, while D20 with 10 % DMSO was the optimal combination for RCC sperm. After ultra-low temperature preservation, the longevity, fertilization, and hatching rates of frozen sperm were significantly lower (<em>P</em> &lt; 0.05) compared to fresh sperm. The enzyme activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), and creatine kinase (CK) were significantly decreased (<em>P</em> &lt; 0.05) in spermatozoa, whereas they showed a significant increase (<em>P</em> &lt; 0.05) in sperm plasma. Succinate dehydrogenase (SDH) activity was significantly reduced (<em>P</em> &lt; 0.05) in frozen spermatozoa of RCC compared to fresh spermatozoa, and exhibited lower activity in frozen spermatozoa of WCC and WR. Additionally, SDH activity was significantly elevated (<em>P</em> &lt; 0.05) in frozen sperm plasma, and glutathione reductase (GR) activity was significantly lower (<em>P</em> &lt; 0.05) in both frozen sperm plasma and spermatozoa across all three species. The study demonstrated that cryopreservation had a significant effect on the enzyme activities of spermatozoa and sperm plasma in all three species. These findings provide important technical support for the conservation of high-quality fish germplasm resources, particularly for novel varieties resulting from distant hybridization in fish.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 234-242"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}