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GGE Biplot Analysis of Genotype x Environment Interaction and Bean Yield Stability of Arabica Coffee (Coffee arabica L.) Genotypes in Southwestern Ethiopia 阿拉比卡咖啡(Coffee Arabica L.)基因型x环境互作与产量稳定性的GGE双图分析埃塞俄比亚西南部的基因型
Pub Date : 2021-06-29 DOI: 10.11648/j.ajbio.20210903.16
Lemi Beksisa
Coffee is the main Ethiopia’s most important agricultural export crop, which is providing about 25-30% of foreign exchange earnings. The estimation of stability performance of the cultivars becomes most important to detect consistently performing and high yielding genotypes. Eleven Arabica coffee genotypes were evaluated in southwestern part of the Ethiopia across four locations for two consecutive years (2014/15 - 2015/16). The objective of the study was to estimate the presence of the interaction between genotypes and environments; and the stability performance of the coffee cultivars for its bean yield. The experimental design was Randomized Complete Block Design (RCBD) replicated twice in each testing location. Genotypes were evaluated by Genotype main effect and genotype x environment interaction (GGE) biplot. The combined analysis of variance indicated that genotypes, environments and GEI showed highly significant (p<0.01). Total variation explained was 41.63% for environments, 9.31% for genotypes and 32.32% for genotype by environment interaction (GEI). This obviously shows that the effect of the genotypes to the total variance was minimal when compare to the environment and the GEI. GGE biplot grouped the environments into four clusters with five genotypes being the winners in different group of environments. Top yielding cultivars namely; L52/2001 and L55/2001; and E6 (Jimma 2015/16) were identified as an ideal genotype and environment, respectively. In this study, stable genotypes, suitable environment for each of the coffee genotype and environment similarity based on bean yield were identified.
咖啡是埃塞俄比亚最重要的主要农业出口作物,它提供了约25-30%的外汇收入。对品种的稳定性进行评估,是检测高产高产基因型的重要手段。连续两年(2014/15 - 2015/16)在埃塞俄比亚西南部的四个地点对11种阿拉比卡咖啡基因型进行了评估。该研究的目的是估计基因型和环境之间相互作用的存在;并对咖啡品种的稳定性表现进行了评价。实验设计采用随机完全区设计(RCBD),每个试验点重复2次。采用基因型主效应和基因型与环境相互作用(GGE)双图评价基因型。综合方差分析显示,基因型、环境和GEI呈极显著差异(p<0.01)。环境因子、基因型因子和基因型因子的总变异解释率分别为41.63%、9.31%和32.32%。这显然表明,与环境和GEI相比,基因型对总方差的影响是最小的。GGE双标图将环境分为四类,在不同的环境组中有五种基因型是优胜者。即高产品种;L52/2001及L55/2001;E6 (Jimma 2015/16)分别为理想基因型和理想环境。在本研究中,确定了稳定的基因型、每个咖啡基因型的适宜环境以及基于咖啡豆产量的环境相似性。
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引用次数: 1
Performance and Adaptability Evaluation of Improved Onion Varieties (Allium cepa L.) for Bulb Yield and Some Agronomic Traits 洋葱改良品种(Allium cepa L.)鳞茎产量及部分农艺性状的表现及适应性评价
Pub Date : 2021-06-16 DOI: 10.11648/j.ajbio.20210903.15
Awoke Ali Zeleke, Demis Fikre Limeneh, Dasta Tsagaye Galalcha, Gizaw Wegayehu Tilahun, N. Hunde, F. Mengistu
Onion is one of the most important vegetable crops in the world including Ethiopia. The field experiment was conducted in Arsi zone at Kulumsa Agriculture Research Center and Ziway Dugda districts under irrigation condition. A total of five improved onion varieties were evaluated in Randomized Complete Block Design with three replications with the objectives of studying the performance and adaptability of improved onion varieties for bulb yield and yield related traits in 2017 during off season. The combined Analysis of variance (variety by location) revealed that significant difference between improved onion varieties for maturity date, marketable and unmarketable bulb yield tons per hectare, bulb length in centimeter, and bulb weight in gram was observed. But non-significant difference for plant height in centimeter, total bulb yield tons per hectare and average bulb diameter in centimeter were observed between improved onion varieties. Except variety Adama Red all the tested improved onion varieties (Melkam, Nafis, Nasik Red and Bombay Red) was well performed and adapted. These varieties were gave more than 40 marketable bulb yield t ha-1. So those improved onion varieties were recommended for the study area and areas of similar agro- ecological zones under off season growing condition.
洋葱是世界上最重要的蔬菜作物之一,包括埃塞俄比亚。在灌溉条件下,在库鲁姆萨农业研究中心Arsi区和Ziway Dugda区进行了田间试验。采用3个重复的随机完全区组设计,对5个洋葱改良品种进行了评价,研究了改良品种在2017年淡季对洋葱鳞茎产量及产量相关性状的表现和适应性。综合方差分析表明,改良洋葱品种在成熟期、可售和滞销鳞茎产量(吨/公顷)、鳞茎长(厘米)和鳞茎重(克)方面存在显著差异。改良洋葱品种的株高(厘米)、总鳞茎产量(吨/公顷)和平均鳞茎直径(厘米)差异不显著。除阿达玛红品种外,所有改良洋葱品种(Melkam、Nafis、Nasik Red和Bombay Red)均表现良好。这些品种的可销售鳞茎产量为每公顷40以上。为研究区及同类农业生态区在淡季生长条件下推荐洋葱改良品种。
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引用次数: 1
Study of Blood Serum Biochemical Profile and Pathological Changes in Haemonchosis Experimentally Induced in Goats 山羊实验性血液病血清生化特征及病理变化的研究
Pub Date : 2021-06-15 DOI: 10.11648/j.ajbio.20210903.14
E. Jaheed
Haemonchus contortus (H. contortus) parasite causes various functional derangements in the host body. This experimental study was established to appraise the remedial effect of Balanites aegyptiaca fruit’s ethanolic extract (BAF-EE) on experimentally induced haemonchosis in goats. Blood serum biochemical parameters and pathological changes were perused. Twelve male goats were randomly divided into 4 equal groups, G1 (infected-untreated), G2 (infected- BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Experimental infection was conducted with a single oral dose of 10x3 infective third larval stage (L3) of H. contortus at 0-time, whereas treatment with BAF-EE and albendazole was given at a single oral dose of 9g and 5mg/kg body wieght respectively in the 5th week post infection (PI). Blood samples were drawn on zero time, 3rd week PI and then biweekly till the 9th week for serum biochemical analyses. At the end of the experiment, all animals were slaughtered to estimate histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum protein (TP), albumin, albumin/globulin (A/G) ratio, total antioxidant capacity (TAC) and calcium (Ca) levels compared to uninfected goats. Significant elevation was observed in values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (γGT), cholesterol, urea and creatinine of infected animals, while no significant differences were recorded in levels of inorganic phosphorus (P), potassium (K), sodium (Na), chloride (Cl) and total iron binding capacity (TIBC). Histopathological studies conducted on abomasa of infected-untreated animals revealed inflammation, necrosis, petechial hemorrhages and intense cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Histopathological alterations in tissues of infected – treated animals were lower in intensity than those of infected-untreated goats. Results of the present experiment demonstrated that BAF-EE has an anti-parasitic and hepatoprotective effects as well as it improves serum biochemical alterations and oxidative stress in goats infected with H. contortus parasite. The present findings nominate the use of this plant as a novel safe natural anthelmintic agent.
弓形血蜱(Haemonchus contortus)寄生在宿主体内引起各种功能紊乱。本试验旨在评价埃及巴兰果乙醇提取物(BAF-EE)对实验性山羊血液病的治疗作用。观察血清生化指标及病理变化。将12只公山羊随机分为4组,G1组(感染-未感染)、G2组(感染-BAF-EE组)、G3组(感染-阿苯达唑组)和G4组(未感染-BAF-EE组)。实验于感染后第5周(PI)单次口服10 × 3个感染第三幼虫期(L3),感染后第5周分别口服BAF-EE和阿苯达唑,剂量分别为9g和5mg/kg体重。0周、PI第3周抽取血样,每两周抽取一次,直至第9周进行血清生化分析。在实验结束时,屠宰所有动物以评估皱胃和肝脏组织的组织病理学改变。血清生化指标显示,与未感染山羊相比,感染山羊血清总蛋白(TP)、白蛋白、白蛋白/球蛋白(A/G)比、总抗氧化能力(TAC)和钙(Ca)水平显著降低。感染动物的天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、γ -谷氨酰转移酶(γGT)、胆固醇、尿素和肌酐均显著升高,而无机磷(P)、钾(K)、钠(Na)、氯(Cl)和总铁结合容量(TIBC)水平无显著差异。经组织病理学检查,感染未处理动物的胃粘膜出现炎症、坏死、点状出血和强烈的细胞浸润。肝脏表现为胆管增生、肝细胞变性及细胞浸润。感染处理过的山羊的组织病理学改变强度低于未处理过的山羊。本实验结果表明,BAF-EE具有抗寄生虫和保护肝脏的作用,并能改善山羊感染弓形螺旋体寄生虫后的血清生化改变和氧化应激。目前的研究结果表明,这种植物是一种新的安全的天然驱虫剂。
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引用次数: 3
Evaluation of NPSB Fertilizer Levels on Yield and Yield Components of Open Pollinated Maize (Zea mays L.) Varieties Under Irrigated Condition in South Omo Zone NPSB施肥水平对开放授粉玉米产量及产量构成的影响南奥莫地区灌溉条件下的品种
Pub Date : 2021-06-10 DOI: 10.11648/j.ajbio.20210903.13
Awoke Tadesse, M. Sultan
Maize is a significant cereal crop in Ethiopia. However, the yield of this crop is limited due to lack of varieties and blended fertilizer for varieties and site specifications. The use of exact amount of fertilizer based on crop requirement has significant importance for sustainable crop production. A study was undertaken to investigate the effects of NPSB blended fertilizer rate for maize yield production in Dasenech and Nyangatom districts in the lowland area of South Omo Zone, Southern Ethiopia during the 2019/2020 cropping season. The experiment was factorial with three maize varieties (Melkassa-6Q Melkassa-4 and Melkassa-2) and four NPSB blended fertilizer rates including kg ha-1 (none fertilizer, 50NPSB, 100NPSB and 150 NPSB). Factorial combinations were used as twelve treatments laid out in a randomized complete block design with three replications. Growth, yield and yield components parameters were recorded and carried out statistical analysis The result of analysis of variance revealed that the interaction effect of varieties and blended fertilizer rate highly significant difference in grain yield. The result showed that the highest grain yield (3913.3 kg ha-1) and net benefit (49,229.6 Eth-birr ha-1) with MRR of 2525.83% were obtained from Melkassa-2 variety treated with 100 kg ha-1of NPSB fertilizer rates at Dasenech location. Whereas, at Nyangatom location, the highest grain yield (4906.7 kg ha-1) and net benefit (62640.45Eth-birr ha-1) with MRR of 2926.2% were obtained from similar Melkassa-2 variety treated with 100 kg ha-1 of NPSB fertilizer levels s. Therefore, based on the result recorded from this study Melkassa-2 varieties treated with 100 kg ha-1 of NPSB blended fertilizer can be suggested as profitable for the production of maize at both districts of the study areas and their similar soil conditions and agro-ecology.
玉米是埃塞俄比亚重要的谷类作物。然而,由于缺乏品种和混合肥料的品种和场地规格,这种作物的产量受到限制。根据作物的需要量合理施用化肥,对作物的可持续生产具有重要意义。在埃塞俄比亚南部南奥莫区低地地区的Dasenech和Nyangatom地区,开展了一项研究,调查了2019/2020种植季NPSB混合肥料对玉米产量的影响。试验采用3个玉米品种(Melkassa-6Q、Melkassa-4和Melkassa-2)和4种NPSB混合肥料(kg hm -1)(无肥、50NPSB、100NPSB和150 NPSB)进行因子试验。阶乘组合作为12个处理,采用随机完全区设计,有3个重复。记录生长、产量和产量组成参数并进行统计分析。方差分析结果表明,品种间互作效应和混交量对籽粒产量的影响极显著。结果表明,在Dasenech地点,施用100 kg ha-1 NPSB肥的Melkassa-2品种籽粒产量最高(3913.3 kg ha-1),净效益最高(49229.6 eh -birr ha-1), MRR为2525.83%。而在Nyangatom位置,相同品种的Melkassa-2在100 kg ha-1的NPSB水平下获得最高的产量(4906.7 kg ha-1)和净效益(62640.45 h-birr ha-1), MRR为2926.2%。根据本研究记录的结果,在研究区土壤条件和农业生态相似的两个地区,施用100 kg ha-1 NPSB混肥处理的Melkassa-2品种对玉米生产有利。
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引用次数: 2
Phenotypic Diversity of Ethiopian Coffee (Coffea arabica L.) Accessions Collected from Limmu Coffee Growing Areas Using Multivariate Analysis 埃塞俄比亚咖啡(Coffea arabica L.)的表型多样性用多变量分析收集Limmu咖啡种植区的材料
Pub Date : 2021-05-21 DOI: 10.11648/J.AJBIO.20210903.12
Lemi Beksisa, T. Benti, Getachew Weldemichael
Forty seven Coffea arabica L. germplasm accessions collected from Limmu district were field evaluated from 2004/5 to 2013/14 with two commercial check varieties at Agaro Agricultural Research sub center in single plot. The objective of the experiment was to assess the variability among the accessions using quantitative traits. Data for about eight quantitative traits were recorded only once in experimental period, while the yield data were recorded for six consecutive cropping seasons. Cluster, genetic divergence, and principal component analysis were used to assess the variability among the genotypes. The results revealed that average linkage cluster analysis for nine traits grouped the germplasm accessions in to three clusters. The number of accessions per cluster ranged from three in cluster III to 25 in cluster II. The clustering pattern of the coffee accessions revealed that the prevalence of moderate genetic diversity in Limmu coffee for the characters studied. The maximum inter-cluster distance was observed between clusters II and III; whereas the minimum inter-cluster distance was observed between clusters I and II. The study highlighted the possibility of using accessions of the distant clusters as potential candidates for the genetic improvement of Limmu coffee through hybridization. Moreover, the principal component analysis showed that the first four PCs extracted about 82% of the total variance of the 49 coffee germplasm accessions and also confirmed moderate diversity among the germplasm accessions since the entire variation cannot be explained in terms of few PCs. This, in turn, indicates that the involvement of a number of traits in contributing towards the overall diversity.
2004/5 ~ 2013/14年在Limmu地区采集的47份阿拉比卡咖啡种质资源,在Agaro农业研究分中心单小区用2个商业对照品种进行田间评价。本试验的目的是利用数量性状评价各种质间的变异。8个数量性状的数据在试验期内仅记录一次,而产量数据则连续记录6个种植季。采用聚类分析、遗传分化分析和主成分分析来评估基因型间的变异。结果表明,9个性状的平均连锁聚类分析将种质资源归为3个聚类。每个集群的加入数量从第三类的3个到第二类的25个不等。咖啡材料的聚类模式表明,所研究的性状在Limmu咖啡中普遍存在中等程度的遗传多样性。聚类II和聚类III之间的簇间距离最大;而簇I和簇II之间的簇间距离最小。该研究强调了利用远缘簇的加入作为Limmu咖啡通过杂交进行遗传改良的潜在候选者的可能性。此外,主成分分析表明,前4个pc提取了49份咖啡种质资源总方差的82%左右,也证实了种质资源之间的适度多样性,因为整个变异不能用少数pc来解释。这反过来又表明,一些特征的参与有助于整体的多样性。
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引用次数: 1
Low-cost Rice Husk Ash and Silica for Chromium Ion Sorption from Aqueous System: Characterisation and Kinetics 低成本稻壳灰和二氧化硅对水系统中铬离子的吸附:表征和动力学
Pub Date : 2021-04-29 DOI: 10.11648/J.AJBIO.20210903.11
Adelagun Ruth Olubukola Ajoke
Several materials have been developed and used to remove Chromate (VI) from industrial wastewater before discharge into the environment because of its toxicity and lethality. In this study, low-cost adsorbents (rice husk ash (RHA) and silica (RHS)) were prepared from rice husks purchased locally from a rice mill industry and characterised. While the crystallinity and mineralogy of the adsorbents were analysed using powdered XRD crystallography, analysis of the physico-chemical properties was performed using standard procedures. Elemental analysis (CHN) was done using Perkin Elmer CHN elemental analyser and FTIR Spectrometer was used to determine the functional groups on the surface of the adsorbents at room temperature. The prepared samples were used as adsorbents for the removal of chromate (VI) ion in a batch sorption process with reaction conditions vis–a-viz adsorbent dosage = 0.5 g, adsorbate volume = 500 ml, adsorbate solution concentrations: 10-200 mg/l, time = 4 h, temperature = ambient temperature. The residual Cr (VI) concentration in the solution was analyzed spectrophotometrically at λ = 540 nm following the 1,5–diphenylcarbazide procedure. Results obtained for the characterisation of the adsorbents were similar and comparable with what obtained in literatures. Physico - chemical analysis revealed a high ash content of 89% and silica content of 95.83%. XRD analysis for the rice husk ash samples reflected the presence of cristobalite (SiO2) in amorphous form and a characteristics broad peak at 2θ angle = 26.5°. FTIR analysis revealed the presence of silanol groups (Si-OH), silicic acid (Si-O-Si-OH) in the ash and Si-O-Si and Si-O) in the silica. The results of effect of time-concentration experiments of chromium ion sorption by the adsorbents revealed that Cr (VI) ion sorption was both time and initial solution concentration dependent with over 80% removal achieved within the first 30 min of contact for both adsorbents as the initial chromate concentration was increased. Kinetic modeling of the process showed up for pseudo second order, hinting on chemisorption as the mechanism of interaction.
由于工业废水中铬酸盐(VI)具有毒性和致死率,人们开发了几种材料来去除工业废水中的铬酸盐(VI)。在这项研究中,低成本吸附剂(稻壳灰(RHA)和二氧化硅(RHS))是从当地购买的稻壳中制备的,并对其进行了表征。使用粉末XRD晶体学分析吸附剂的结晶度和矿物学,使用标准程序分析其物理化学性质。采用Perkin Elmer元素分析仪进行元素分析(CHN),并用FTIR光谱仪在室温下测定吸附剂表面的官能团。将制备的样品作为吸附剂,在吸附剂用量为0.5 g,吸附剂体积为500 ml,吸附剂溶液浓度为10-200 mg/l,时间为4 h,温度为环境温度的条件下,对铬酸盐(VI)离子进行了间歇吸附。采用1,5 -二苯脲法,在λ = 540 nm处分光光度法测定了溶液中残留Cr (VI)的浓度。所得到的吸附剂的表征结果与文献中所得到的相似和可比较。理化分析表明其灰分含量高达89%,二氧化硅含量高达95.83%。稻壳灰分样品的XRD分析反映出方石英(SiO2)以无定形存在,且在2θ角= 26.5°处有一个特征宽峰。FTIR分析显示灰分中存在硅醇基团(Si-OH)和硅酸(Si-O-Si-OH),二氧化硅中存在硅- o - si和硅- o。吸附材料对铬离子吸附的时间-浓度实验结果表明,Cr (VI)离子的吸附与时间和初始溶液浓度有关,随着初始铬酸盐浓度的增加,两种吸附剂在接触后30 min内的去除率均超过80%。该过程的动力学模型显示为准二级,暗示化学吸附是相互作用的机制。
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引用次数: 1
Genotype × Environment Interaction and Selection of High Yielding Wheat Genotypes for Different Wheat-growing Areas of Ethiopia 埃塞俄比亚不同小麦产区高产小麦基因型的基因型与环境互作及选择
Pub Date : 2021-04-23 DOI: 10.11648/J.AJBIO.20210902.15
Gadisa Alemu, Alemu Dabi, Nagesh Geleta, R. Duga, Tafesse Solomon, Habte Zegaye, Abebe Getamesay, Abebe Delesa, Dawit Asnake, Bayisa Asefa, Y. Shewaye, B. Abeyo, A. Badebo
Evaluation of wheat genotypes under different environments is essential for testing stability of their performance and range of adaptations. Where, enhanced grain yield has been achieved in bread wheat (Tritium aestivum L.) through the development of adaptable, high-yielding, and rust-resistant genotypes by evaluating advanced genotypes for yield in multi-environment trials. The adaptability of a genotype over diverse environments is usually tested by the degree of its interaction with different environments under which it is grown. This study examines fifteen bread wheat genotypes for two consecutive years (2016 and 2017) across eight locations in Ethiopia. The experiment was laid out using a Randomized Complete Block design and replicated three times intending to determine high-yielding advanced genotype and release best performing genotypes as a variety for the end-user. Highly significant (P ≤ 0.001) effects of genotype, environment, and genotype by environment interaction were observed for grain yield. The AMMI analysis of variance indicated that environments accounted for 52.34% of the total sum of squares for grain yield (genotype yields ranged from 5.76 t/ha at E-11 to 1.31 t/ha at E-7). followed by interaction (22.95%) and genotypes (11.31%).The genotype ETBW8260 (G4) exhibited high mean grain yield and well performed to the tested environments. The ETBW8260 (G4) was selected as early maturing, high yielding, resistant to yellow rust and fitting for low to midland wheat growing areas and has a yield advantage over the standard Ogolcho and the local Kakaba. Both locations and genotypes are dispersed widely in all quadrants in the AMMI-1 biplot. As per the YSI Ogolcho (#15) ETBW8303 (G2), ETBW8454 (G12), ETBW8261 (G5), ETBW8406 (G10), ETBW8310 (G3), and ETBW8260 (G4), the genotypes of bread wheat were stable. Besides, the genotype ETBW8260 (G4 has highest mean yield with good stability. Therefore, after a one-year variety verification trial, ETBW8260 is released as a commercial variety in 2019 and a designated local name called “Balcha” and recommended for low-medium part of wheat production agroecology of the country.
对不同环境下的小麦基因型进行评价是检验其性能稳定性和适应范围的必要条件。其中,通过在多环境试验中评估先进基因型的产量,通过开发适应性强、高产和抗锈病的基因型,面包小麦(Tritium aestivum L.)实现了增产。一个基因型对不同环境的适应性通常是通过它与不同生长环境的相互作用程度来检验的。本研究连续两年(2016年和2017年)对埃塞俄比亚8个地点的15种面包小麦基因型进行了研究。试验采用随机完全区设计,重复三次,旨在确定高产高级基因型,并将表现最佳的基因型作为最终用户的品种。基因型、环境和环境互作对粮食产量的影响极显著(P≤0.001)。AMMI方差分析表明,环境因子占籽粒产量平方和的52.34% (E-11基因型产量为5.76 t/ha ~ E-7基因型产量为1.31 t/ha)。其次是相互作用(22.95%)和基因型(11.31%)。基因型ETBW8260 (G4)表现出较高的平均产量和对试验环境的良好适应。选择ETBW8260 (G4)作为早熟、高产、抗黄锈、适合中低小麦产区的品种,其产量优于标准品种Ogolcho和当地品种Kakaba。AMMI-1双象限的位置和基因型分布广泛。根据YSI Ogolcho(15号)ETBW8303 (G2)、ETBW8454 (G12)、ETBW8261 (G5)、ETBW8406 (G10)、ETBW8310 (G3)和ETBW8260 (G4),面包小麦的基因型较为稳定。此外,基因型ETBW8260 (G4)的平均产量最高,稳定性好。因此,经过一年的品种验证试验,ETBW8260于2019年作为商品品种发布,并指定当地名称为“Balcha”,推荐用于国家小麦生产中低农业生态部分。
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引用次数: 3
The Livability of Bovans Brown Parent Layers Raised in Commercial Breeder Farm, Ethiopia 埃塞俄比亚商业种鸡场饲养的Bovans褐蛋鸡的宜居性
Pub Date : 2021-04-23 DOI: 10.11648/J.BIO.20210902.11
B. Abera, Kasim Kediro, M. Jafer, Shihun Shimelis, A. Ismael, D. Alemu, Yehualashet Bayu
In order to meet its chicken meat and egg demand for its growing population and produces export surpluses, Ethiopian poultry sub-sector should move away from the traditional scavenging family poultry system (TFP) to the improved semi-scavenging family poultry system (IFP) and increase the scale of specialized poultry. The Bovans brown (BB) genetics are one of familiar exotic breed in Ethiopia. A prospective cohort study was conducted to estimate their livability and evaluate body weight and age as predictors of livability. There is direct proportional trend in body weight increment with slight difference with the standard. Both sex groups showed excellent growth with percent uniformity of 96.7% for males and 95.3% for females. Overall, 161 chickens died with the total weekly chicken death rate ranged from 0.00% to 1.79% varying from 0.00% to 3.14% in males and from 0.00% to 1.57% in females. On the other hand the percentage of cumulative mortality was 9.91% (46/510) for male chicken and 2.94 (115/4020)% for female and 3.69% (161/4530) for all. The mean mortality of chicken was 4.24 per week being 2.42 for males and 6.05 for females. The loss of female chickens exceeded that of male chickens, CV of 2.38 for the former and 1.82 for the latter. Using regression analysis, the identified significant predictors of mortality were age in weeks (Coef. = -0.78; p = 0.011) and body weight in gram (Coef. =-0.0086; p = 0.006). As both parameters get increased, mortality of chicken on the contrary got reduced. The overall livability of bovans brown breeder chicks at the end of rearing period was 96.45% (90.98% for males and 97.14 for females). Bovans brown parent stock layer chicks demonstrated excellent performance and livability.
为了满足其不断增长的人口对鸡肉和鸡蛋的需求并产生出口盈余,埃塞俄比亚家禽分部门应从传统的食腐家庭家禽系统(TFP)转向改进的半食腐家庭家禽系统(IFP),并扩大专业化家禽的规模。Bovans brown (BB)是埃塞俄比亚常见的外来品种之一。进行了一项前瞻性队列研究,以估计它们的宜居性,并评估体重和年龄作为宜居性的预测因子。体重增长呈正比趋势,与标准差异不大。两个性别组均表现出良好的生长,均匀性为男性96.7%,女性95.3%。共有161只鸡死亡,鸡周总死亡率为0.00% ~ 1.79%,雄性为0.00% ~ 3.14%,雌性为0.00% ~ 1.57%。雏鸡累积死亡率分别为9.91%(46/510)、2.94(115/4020)%和3.69%(161/4530)。平均死亡4.24只/周,其中雄鸡2.42只/周,雌鸡6.05只/周。雏鸡的损失大于雄鸡,前者CV为2.38,后者CV为1.82。通过回归分析,确定了死亡率的显著预测因素是周龄(Coef。= -0.78;p = 0.011)和以克为单位的体重(Coef。= -0.0086;P = 0.006)。随着这两个参数的增加,鸡的死亡率反而降低。育成期末期,雄性和雌性的总体存活率分别为96.45%和97.14%。bovan棕色母本产蛋鸡表现出优良的生产性能和宜居性。
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引用次数: 0
Gelsemium Low doses Increases Bioenergetics and Neurite Outgrowth 低剂量的硒增加生物能量和神经突生长
Pub Date : 2021-04-21 DOI: 10.21203/RS.3.RS-377125/V1
I. Lejri, A. Grimm, P. Trempat, N. Boujedaini, A. Eckert
Background: Gelsemium sempervirens (GS) is a traditional medicinal plant, described at ultra-low doses as a remedy for a variety of psychological and behavioral symptoms of anxiety and depression. Changes in neural plasticity have been shown to play a significant role in the onset and development of those mental illnesses. Mitochondria play an extremely important role in the central nervous system by being the main energy producer through the oxidative phosphorylation and being involved particularly in the regulation of cell survival or death, as well as synaptic plasticity. Neurite outgrowth is the differentiation process by which neurons establish synapses through the protrusion of neurons and their extension. Methods: Because the effects of GS dilutions on mitochondrial function and neuroplasticity remain elusive, we aimed to investigate whether a treatment with GS at low doses (centesimal dilutions, C) improved bioenergetics parameters such as ATP production, mitochondrial respiration as well as cellular glycolysis before to characterize its effects on neurite outgrowth. Nerve growth factor (NGF), which is known as a promotor of cell growth and survival, was used as a positive control. Results: Our results demonstrate that GS dilutions (3C and 5C) efficiently ameliorated the bioenergetics of SH-SY5Y neuroblastoma cells by increasing cellular ATP level and mitochondrial respiration as well as promoting the cell survival. In addition, GS dilutions significantly improved neurite extension in the 2D as well as 3D culture model after 3 days of treatment. 3C and 5C dilutions showed similar functional effects than those obtained with the positive control nerve growth factor (NGF). Conclusions: These findings indicate that GS dilutions modulate the mitochondrial bioenergetic phenotype and improve the neurite formation. The mitochondrial function improving properties of GS dilutions might represent one possible important pathway contributing to its neuroprotective effectiveness. Key words: Gelsemium dilutions, mitochondria, bioenergetics, neurite outgrowth.
背景:格尔塞姆(Gelsemium sempervirens, GS)是一种传统的药用植物,在超低剂量下被描述为治疗焦虑和抑郁的各种心理和行为症状。神经可塑性的变化已被证明在这些精神疾病的发生和发展中起着重要作用。线粒体在中枢神经系统中发挥着极其重要的作用,它通过氧化磷酸化产生主要的能量,尤其参与调节细胞的生存或死亡,以及突触的可塑性。神经突生长是神经元通过突起和延伸形成突触的分化过程。方法:由于GS稀释剂对线粒体功能和神经可塑性的影响仍然难以捉摸,我们的目的是研究低剂量(百分位数稀释,C)的GS治疗是否能改善生物能量学参数,如ATP产生、线粒体呼吸和细胞糖酵解,然后表征其对神经突生长的影响。神经生长因子(NGF)被认为是细胞生长和存活的促进剂,作为阳性对照。结果:我们的研究结果表明,GS稀释(3C和5C)通过增加细胞ATP水平和线粒体呼吸,有效改善SH-SY5Y神经母细胞瘤细胞的生物能量,促进细胞存活。此外,GS稀释剂在治疗3天后显著改善了2D和3D培养模型中的神经突延伸。3C和5C稀释后的功能效果与阳性对照神经生长因子(NGF)相似。结论:这些结果表明,GS稀释可调节线粒体生物能表型并改善神经突的形成。GS稀释液改善线粒体功能的特性可能是其神经保护作用的一个重要途径。关键词:凝胶稀释,线粒体,生物能学,神经突生长
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引用次数: 0
In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats 双芒花树皮水提物对wistar大鼠体外抗氧化及抗关节炎活性的研究
Pub Date : 2021-04-07 DOI: 10.11648/J.AJBIO.20210902.14
Ouattara-Soro Fatou Shcherazade, Kouadio Kouakou John, Yao Konan Bertin, Thanon Mariam, Abizi Georges
Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.
benthamianus是Caesalpiniaceae家族的一种植物,在传统医学中用于治疗炎症问题,如风湿病,支气管炎,癫痫和煮沸。本研究的目的是通过评价该植物树皮对wistar大鼠的体外抗氧化和抗关节炎作用来开发其药理作用。通过DPPH自由基清除试验测定提取物的抗自由基活性和还原活性来评价提取物的抗氧化活性。以Wistars大鼠为实验对象,评价其抗关节炎活性。在大鼠左腿足底筋膜处注射弗洛伊德完全佐剂诱导大鼠关节炎。提取液的还原力为426.0±17.31µmol Eq/Trolox/g EXS, IC50为26.67±1.12µg/mL。植物提取物和参比分子给予关节炎大鼠明显减轻水肿和关节炎症状。大鼠的体重很快稳定在治疗大鼠的水平上。实验结束时,提取物在200、400、800 mg/kg/bw的抑制率分别为38.2%、44.33%和48%。双氯芬酸钠和强的松分子(5 mg/kg/bw)的抑制率分别为47.33%和37.84%。部分从水肿减轻来看,治疗大鼠血液学(白细胞、血小板)和生化(纤维蛋白原、总蛋白、白蛋白、总胆红素和偶联胆红素、AST、ALT)指标与健康大鼠比较无显著差异。这些结果证实了双芒菊树皮在慢性炎症性疾病中的传统应用。
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引用次数: 0
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American Journal of BioScience
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