Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.45783.1302
Noha El Najdi, M. Mostafa, Eman Mohamed
{"title":"Evaluation of Sealing Ability of Totalfill versus ProRoot MTA in Furcal Perforations in Permanent Molars Using Scanning Electron Microscope (SEM)","authors":"Noha El Najdi, M. Mostafa, Eman Mohamed","doi":"10.21608/adjg.2022.45783.1302","DOIUrl":"https://doi.org/10.21608/adjg.2022.45783.1302","url":null,"abstract":"","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90542334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.123895.1467
S. Bahaa, Sara M. Shabaan
Purpose : This study aimed to evaluate the effect of microwave disinfection protocol on dimensional changes and the pattern of occlusal pressure over lined complete dentures. Material and methods: ten completely edentulous patients were selected to receive conventional complete dentures. A layer of soft liner was added to the lower dentures and occlusal pressure contacts were recorded using T-scan device. The lower dentures were subjected to microwave radiation 700 Watt for three minutes once a week for four successive weeks in a dry condition. Twenty maxillary denture bases were constructed and lined with soft liner, the gap between the lined denture base and the stone cast was measured at the posterior palatal seal area at three different points, as well as the occlusal pattern before and after exposure to microwave radiation. Results: there were no significant effect for using microwave radiation on the lined denture regarding occlusal force distribution and dimensional changes. Conclusion: lined denture disinfection by microwave energy at 700watt for three minutes has no statistically significant effect over the dimensional changes and occlusal force distribution.
{"title":"Evaluation of Occlusal Pressure Pattern and Dimensional Changes of the Lined Dentures Subjected to Microwave Disinfection Protocol","authors":"S. Bahaa, Sara M. Shabaan","doi":"10.21608/adjg.2022.123895.1467","DOIUrl":"https://doi.org/10.21608/adjg.2022.123895.1467","url":null,"abstract":"Purpose : This study aimed to evaluate the effect of microwave disinfection protocol on dimensional changes and the pattern of occlusal pressure over lined complete dentures. Material and methods: ten completely edentulous patients were selected to receive conventional complete dentures. A layer of soft liner was added to the lower dentures and occlusal pressure contacts were recorded using T-scan device. The lower dentures were subjected to microwave radiation 700 Watt for three minutes once a week for four successive weeks in a dry condition. Twenty maxillary denture bases were constructed and lined with soft liner, the gap between the lined denture base and the stone cast was measured at the posterior palatal seal area at three different points, as well as the occlusal pattern before and after exposure to microwave radiation. Results: there were no significant effect for using microwave radiation on the lined denture regarding occlusal force distribution and dimensional changes. Conclusion: lined denture disinfection by microwave energy at 700watt for three minutes has no statistically significant effect over the dimensional changes and occlusal force distribution.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"1995 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82444240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.136443.1501
Ola A. Sakr, M. Mandour, Zahraa Gabal
Purpose: The aim of this study was to compare fracture resistance of provisional restorations fabricated with conventional manual procedure, CAD/CAM milling and 3D Printing techniques. Materials and Methods : one human upper first premolar was prepared and duplicated using epoxy resin to produce twenty one dies. Accordingly, a total of (N=21) provisional crowns were constructed. The Constructed provisional restorations were divided into 3 groups according to the method of construction (n=7 for each group), Group 1: Provisional crowns were constructed using conventional manual technique, Group2: Provisional crowns were constructed using CAD/CAM milling technique, Group3: Provisional crowns were constructed using 3D Printing technique. All Provisional crowns were bonded to their corresponding epoxy resin dies by using CharmTemp ZONE temporary cement and subjected to thermocycling procedure simulating approximately one month of clinical situations Results: Statistical analysis showed that the fracture resistance of CAD/CAM samples (group 2), recorded the statistically significant highest mean fracture resistance value (910.20 ± 118.95), followed by 3D Printing samples (group 3) (720.80 ± 129.57), while the lowest value was recorded for Conventional samples (group 1) (626.71 ± 103.23). ANOVA test showed that there was no significant difference between 3D Printing samples (group 3) and Conventional samples (group 1). Conclusion: CAD/CAM provisional crowns have superior strength than 3D Printing and conventional crowns.
{"title":"Fracture Resistance of Provisional Crowns Fabricated by Conventional, CAD/CAM, and 3D Printing Techniques","authors":"Ola A. Sakr, M. Mandour, Zahraa Gabal","doi":"10.21608/adjg.2022.136443.1501","DOIUrl":"https://doi.org/10.21608/adjg.2022.136443.1501","url":null,"abstract":"Purpose: The aim of this study was to compare fracture resistance of provisional restorations fabricated with conventional manual procedure, CAD/CAM milling and 3D Printing techniques. Materials and Methods : one human upper first premolar was prepared and duplicated using epoxy resin to produce twenty one dies. Accordingly, a total of (N=21) provisional crowns were constructed. The Constructed provisional restorations were divided into 3 groups according to the method of construction (n=7 for each group), Group 1: Provisional crowns were constructed using conventional manual technique, Group2: Provisional crowns were constructed using CAD/CAM milling technique, Group3: Provisional crowns were constructed using 3D Printing technique. All Provisional crowns were bonded to their corresponding epoxy resin dies by using CharmTemp ZONE temporary cement and subjected to thermocycling procedure simulating approximately one month of clinical situations Results: Statistical analysis showed that the fracture resistance of CAD/CAM samples (group 2), recorded the statistically significant highest mean fracture resistance value (910.20 ± 118.95), followed by 3D Printing samples (group 3) (720.80 ± 129.57), while the lowest value was recorded for Conventional samples (group 1) (626.71 ± 103.23). ANOVA test showed that there was no significant difference between 3D Printing samples (group 3) and Conventional samples (group 1). Conclusion: CAD/CAM provisional crowns have superior strength than 3D Printing and conventional crowns.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84582890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.124429.1472
Hind Mohmed, M. Attia, Lobna Abd-Alaziz, A. Dawaba, N. Bakr
Purpose: the present study was conducted to compare the efficacy of herbal treatment using Aloe Vera in induced lichenoid reaction versus topical steroids. Materials and methods : thirty-six rats were divided into four groups: 1 st group control normal group with no induced oral lesions, 2 nd group (Negative control group) with induced oral lesions but no treatment was used, 3 rd group (Positive control group) treated with triamcinolone acetonide 0.1% paste, in the 4 th group (Aloe vera group) treated with topical Aloe vera gel, rats in the 3 rd and 4 th groups are treated topically for 15 days, samples were taken from the buccal mucosa after the end of the study and sent for histological examination. Results: Histological sections revealed there was a decrease of inflammatory and dysplastic changes of the 3 rd and 4 th groups in comparison to the 2 nd group which received no treatment. Conclusion: Aloe vera might be considered as an accepted alternative treatment option due to their anti-inflammatory, anti-oxidant and anti-neoplastic properties with compared to steroid therapy.
{"title":"Efficacy of Aloe vera in Treatment of Experimentally Induced Oral Lichenoid Reaction","authors":"Hind Mohmed, M. Attia, Lobna Abd-Alaziz, A. Dawaba, N. Bakr","doi":"10.21608/adjg.2022.124429.1472","DOIUrl":"https://doi.org/10.21608/adjg.2022.124429.1472","url":null,"abstract":"Purpose: the present study was conducted to compare the efficacy of herbal treatment using Aloe Vera in induced lichenoid reaction versus topical steroids. Materials and methods : thirty-six rats were divided into four groups: 1 st group control normal group with no induced oral lesions, 2 nd group (Negative control group) with induced oral lesions but no treatment was used, 3 rd group (Positive control group) treated with triamcinolone acetonide 0.1% paste, in the 4 th group (Aloe vera group) treated with topical Aloe vera gel, rats in the 3 rd and 4 th groups are treated topically for 15 days, samples were taken from the buccal mucosa after the end of the study and sent for histological examination. Results: Histological sections revealed there was a decrease of inflammatory and dysplastic changes of the 3 rd and 4 th groups in comparison to the 2 nd group which received no treatment. Conclusion: Aloe vera might be considered as an accepted alternative treatment option due to their anti-inflammatory, anti-oxidant and anti-neoplastic properties with compared to steroid therapy.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74667343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.76184.1384
Esraa Ibrahim, H. Adawy, N. Lasheen, Mena Abd Allah
Purpose: This study aimed to evaluate the effect of local and intravenous administration of adipose tissue-derived stem cells on the regeneration of oral epithelium in chemotherapy-treated rats. Material and Methods: 48 adult male albino rats were used in this study throughout the experimental period (2 weeks). 12 rats were left as negative controls (G I) and the remaining experimental 36 rats were treated with 5-fluo-rouracil (chemotherapeutic agent) in a dose of 50 mg/kg/dose to induce oral mucositis. Moreover, acetic acid was injected in the buccal mucosa to produce ulcers and then the rats were subdivided into three subgroups, (G II) in which 12 rats received no treatment, (G III) in which 12 rats received a direct injection of a single dose of adipose-derived stem cells, (G IV) in which 12 rats received an intravenous injection of a single of adipose-derived stem cells. All rats were euthanized; cheek epithelium was dissected and processed for hematoxylin & eosin evaluation. Results: Group III produced better healing than group IV where the ulcer appeared covered with a continuous layer of regenerated epithelium after one week in group III while the regenerating epithelium failed to cover the ulcer in group IV. After two weeks, the epithelium covered the ulcers in both groups but the C.T. architecture appeared better in group III. Conclusion: The direct injection of ADSCs in oral mucositis produces faster healing potentiality than intravenous injection.
{"title":"Evaluation of Local Versus Intravenous Administration of Adipose Tissue-Derived Stem Cells on Regeneration of Oral Epithelium of Chemotherapy Treated Rats","authors":"Esraa Ibrahim, H. Adawy, N. Lasheen, Mena Abd Allah","doi":"10.21608/adjg.2022.76184.1384","DOIUrl":"https://doi.org/10.21608/adjg.2022.76184.1384","url":null,"abstract":"Purpose: This study aimed to evaluate the effect of local and intravenous administration of adipose tissue-derived stem cells on the regeneration of oral epithelium in chemotherapy-treated rats. Material and Methods: 48 adult male albino rats were used in this study throughout the experimental period (2 weeks). 12 rats were left as negative controls (G I) and the remaining experimental 36 rats were treated with 5-fluo-rouracil (chemotherapeutic agent) in a dose of 50 mg/kg/dose to induce oral mucositis. Moreover, acetic acid was injected in the buccal mucosa to produce ulcers and then the rats were subdivided into three subgroups, (G II) in which 12 rats received no treatment, (G III) in which 12 rats received a direct injection of a single dose of adipose-derived stem cells, (G IV) in which 12 rats received an intravenous injection of a single of adipose-derived stem cells. All rats were euthanized; cheek epithelium was dissected and processed for hematoxylin & eosin evaluation. Results: Group III produced better healing than group IV where the ulcer appeared covered with a continuous layer of regenerated epithelium after one week in group III while the regenerating epithelium failed to cover the ulcer in group IV. After two weeks, the epithelium covered the ulcers in both groups but the C.T. architecture appeared better in group III. Conclusion: The direct injection of ADSCs in oral mucositis produces faster healing potentiality than intravenous injection.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80267404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.138322.1513
A. Mostafa, Noha H El-Shaheed
Purpose: Patient satisfaction for the complete denture constructed by using two impression techniques was assessed. A custom dual tray was used as a modifica-tion of window impression technique versus the conventional impression technique. Subjects and methods: Ten completely edentulous patients with anterior hypermobile tissues were included in this study, using a non-randomized cross-over study design. The aim of the study was to evaluate patient satisfaction with two maxillary complete dentures constructed for each patient, one using the windows impression approach with a custom-made dual tray and the other using the conventional impression technique with a custom single tray. The time interval for using each set was about 3 and 6 months. Patients’ satisfactions were assessed at every time interval. Data was analyzed to determine the patient satisfaction with the different complete dentures using the SPSS.ver.23 and Wilcoxon signed rank test at (α = 0.05). Results: Patients’ satisfaction survey showed that the 2 different maxillary dentures were not statistically equivalent, where the maxillary complete denture constructed with windows technique using the dual custom tray demonstrated greater patient satisfaction. Conclusion: Within this study, complete denture constructed with windows technique using the dual custom tray improves the denture function and subsequently the patient satisfaction.
{"title":"Patient Satisfaction with Complete Denture Constructed Using Modified Windows Impression Technique with Custom Dual Tray for hypermobile Maxillary Residual Ridge","authors":"A. Mostafa, Noha H El-Shaheed","doi":"10.21608/adjg.2022.138322.1513","DOIUrl":"https://doi.org/10.21608/adjg.2022.138322.1513","url":null,"abstract":"Purpose: Patient satisfaction for the complete denture constructed by using two impression techniques was assessed. A custom dual tray was used as a modifica-tion of window impression technique versus the conventional impression technique. Subjects and methods: Ten completely edentulous patients with anterior hypermobile tissues were included in this study, using a non-randomized cross-over study design. The aim of the study was to evaluate patient satisfaction with two maxillary complete dentures constructed for each patient, one using the windows impression approach with a custom-made dual tray and the other using the conventional impression technique with a custom single tray. The time interval for using each set was about 3 and 6 months. Patients’ satisfactions were assessed at every time interval. Data was analyzed to determine the patient satisfaction with the different complete dentures using the SPSS.ver.23 and Wilcoxon signed rank test at (α = 0.05). Results: Patients’ satisfaction survey showed that the 2 different maxillary dentures were not statistically equivalent, where the maxillary complete denture constructed with windows technique using the dual custom tray demonstrated greater patient satisfaction. Conclusion: Within this study, complete denture constructed with windows technique using the dual custom tray improves the denture function and subsequently the patient satisfaction.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85928592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.104155.1453
Aya Ahmed, S. Hassan, F. Mohamed, Usama El Dakrory
{"title":"Comparison between Two Computer Designed Custom Made Miniplates for The Treatment of Mandibular Angle Fractures","authors":"Aya Ahmed, S. Hassan, F. Mohamed, Usama El Dakrory","doi":"10.21608/adjg.2022.104155.1453","DOIUrl":"https://doi.org/10.21608/adjg.2022.104155.1453","url":null,"abstract":"","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85304019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/adjg.2022.144555.1516
Amira O. Alkamel, Heba Abozaed, W. Ahmed, F. El-Waseef
Purpose : This cross-over study aimed at assessing masticatory efficiency in single-implant mandibular overdentures with different reinforcement materials (CO-Cr and PEEK). Materials and Methods: Eight completely edentulous participants have been delivered conventional complete dentures. One month later; they received a single midline mandibular implant. After three months of osseointegration, each patient received two mandibular overdentures; one was metal reinforced (Group I) , and the other was PEEK reinforced (Group II) . Masticatory efficiency for both groups (I & II) was carried out. Masticatory efficiency was measured by two colored chewing gums test at time of each overdenture insertion (T0). Thereafter, masticatory efficiency was evaluated subsequent to three months of using metal and PEEK reinforced overdentures (T3). Results: In regard to masticatory efficiency, no significant difference was noted between the studied groups reinforced with metal or PEEK at (T0) and three months after insertion (T3). Among chewing strokes, significant differences were revealed between varied chewing stroke counts among both groups (increased chewing efficiency with an increased number of chewing strokes). Conclusion: Respecting single midline implant overdenture, there was no significant difference between (Metal & PEEK) reinforcement regarding masticatory efficiency. SIMOs reinforced with either Metal or PEEK frameworks could be successful treatment options for assisting mandibular complete overdentures.
{"title":"A Within- Subject Comparison of Two Reinforcement Materials Used for Single Mandibular Implant Retained Overdentures","authors":"Amira O. Alkamel, Heba Abozaed, W. Ahmed, F. El-Waseef","doi":"10.21608/adjg.2022.144555.1516","DOIUrl":"https://doi.org/10.21608/adjg.2022.144555.1516","url":null,"abstract":"Purpose : This cross-over study aimed at assessing masticatory efficiency in single-implant mandibular overdentures with different reinforcement materials (CO-Cr and PEEK). Materials and Methods: Eight completely edentulous participants have been delivered conventional complete dentures. One month later; they received a single midline mandibular implant. After three months of osseointegration, each patient received two mandibular overdentures; one was metal reinforced (Group I) , and the other was PEEK reinforced (Group II) . Masticatory efficiency for both groups (I & II) was carried out. Masticatory efficiency was measured by two colored chewing gums test at time of each overdenture insertion (T0). Thereafter, masticatory efficiency was evaluated subsequent to three months of using metal and PEEK reinforced overdentures (T3). Results: In regard to masticatory efficiency, no significant difference was noted between the studied groups reinforced with metal or PEEK at (T0) and three months after insertion (T3). Among chewing strokes, significant differences were revealed between varied chewing stroke counts among both groups (increased chewing efficiency with an increased number of chewing strokes). Conclusion: Respecting single midline implant overdenture, there was no significant difference between (Metal & PEEK) reinforcement regarding masticatory efficiency. SIMOs reinforced with either Metal or PEEK frameworks could be successful treatment options for assisting mandibular complete overdentures.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84561237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/adjg.2022.151733.1518
May H. EL Mofty
{"title":"Attitude and Knowledge of Orthodontics among Egyptian Patients: A Cross-Sectional Survey","authors":"May H. EL Mofty","doi":"10.21608/adjg.2022.151733.1518","DOIUrl":"https://doi.org/10.21608/adjg.2022.151733.1518","url":null,"abstract":"","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80851126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/adjg.2022.45819.1303
Panseh Abdelfattah, M. Mostafa, Eman Mohamed
Purpose: To assess the effect of Green Tea Extract (GTE) on the periodontal ligament fibroblasts viability comparing to Hank’s Balanced Salt Solution (HBSS). Materials and methods: Periodontal ligament fibroblasts were obtained from 30 freshly extracted sound premolars and cultured in Dulbecco’s Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in Hank’s balanced salt solution (Group I), Green tea extract (Group II) and fresh Dulbecco’s Modified Eagle Medium (DMEM) (Group III) as positive control, for 1,3,6,12,24 and 48h at 37 o C using MTT assay (3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide). Results: Statistical analysis was performed by one-way analysis of variance (ANOVA) and post hoc tests. The findings of this study revealed that there was no significant difference between groups at 1h and 3h time intervals, while GTE showed considerably better protective effect than HBSS at 6 hrs. ( p = 0.002), at 12 hrs. ( p = 0.001), 24 hrs. ( p = 3.38E-03) and 48 hrs. ( p = 1.65E-03). Conclusion: Within the boundaries of the present study, it showed that GTE had higher protective effect on periodontal ligament fibroblasts than HBSS and hence can be used as alternative medium to HBSS.
{"title":"Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution","authors":"Panseh Abdelfattah, M. Mostafa, Eman Mohamed","doi":"10.21608/adjg.2022.45819.1303","DOIUrl":"https://doi.org/10.21608/adjg.2022.45819.1303","url":null,"abstract":"Purpose: To assess the effect of Green Tea Extract (GTE) on the periodontal ligament fibroblasts viability comparing to Hank’s Balanced Salt Solution (HBSS). Materials and methods: Periodontal ligament fibroblasts were obtained from 30 freshly extracted sound premolars and cultured in Dulbecco’s Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in Hank’s balanced salt solution (Group I), Green tea extract (Group II) and fresh Dulbecco’s Modified Eagle Medium (DMEM) (Group III) as positive control, for 1,3,6,12,24 and 48h at 37 o C using MTT assay (3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide). Results: Statistical analysis was performed by one-way analysis of variance (ANOVA) and post hoc tests. The findings of this study revealed that there was no significant difference between groups at 1h and 3h time intervals, while GTE showed considerably better protective effect than HBSS at 6 hrs. ( p = 0.002), at 12 hrs. ( p = 0.001), 24 hrs. ( p = 3.38E-03) and 48 hrs. ( p = 1.65E-03). Conclusion: Within the boundaries of the present study, it showed that GTE had higher protective effect on periodontal ligament fibroblasts than HBSS and hence can be used as alternative medium to HBSS.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"185 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82494430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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