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The Effect of Natural Antioxidants on Free Radicals Clearance after Tooth Bleaching 天然抗氧化剂对牙齿漂白后自由基清除的影响
Pub Date : 2022-07-22 DOI: 10.21608/adjg.2022.75949.1375
Eman Soliman, maha niazy, Fatma F Hussein
Purpose: This in-vitro study aimed to assess the impact of natural antioxidants on free radical’s removal at different time intervals. Materials and methods: A total of sixty intact upper central incisors were randomly split into four groups (n=15 each) according to the antioxidant used and all groups were bleached using gel containing 40% hydrogen peroxide (HP): G1 (control group): Bleaching with no antioxidant. G2: Bleaching followed by 5% grape seed extract solution for 10 mins G3: Bleaching followed by 5% green tea extract solution for 10 mins G4: Bleaching followed by 10% sodium ascorbate solution for 10 mins. The assessment of free radicals for each group was evaluated by colorimetric method at three-time intervals; baseline, after 24 and after 48 hrs. Results: Regarding effect of antioxidants on free radical clearance at different time intervals , results of this study showed that there was statistical substantial disparity between all groups at all time intervals. The greatest mean percentage change was recorded for sodium ascorbate, followed by green tea, then grape seed extract whereas the least value was recorded for the control. Conclusion: All the antioxidants employed in this study were capable of removal of free radicals after bleaching. It was concluded that, sodium ascorbate showed statistically significant decrease in free radicals after tooth bleaching when compared to natural antioxidants.
目的:本体外研究旨在评估天然抗氧化剂在不同时间间隔对自由基清除的影响。材料与方法:将60只完整上颌中切牙按抗氧化剂使用情况随机分为4组,每组15只,均采用含40%过氧化氢(HP)的凝胶进行漂白:G1组(对照组):不加抗氧化剂进行漂白。G2:漂白后用5%葡萄籽萃取液10分钟G3:漂白后用5%绿茶萃取液10分钟G4:漂白后用10%抗坏血酸钠溶液10分钟每隔3个时间间隔用比色法评价各组的自由基;基线,24小时后和48小时后。结果:关于抗氧化剂在不同时间间隔对自由基清除的影响,本研究结果显示各组在不同时间间隔间存在显著的统计学差异。抗坏血酸钠的平均百分比变化最大,其次是绿茶,然后是葡萄籽提取物,而对照组的平均百分比变化最小。结论:本研究所采用的抗氧化剂均能去除脱色后的自由基。结论:与天然抗氧化剂相比,抗坏血酸钠对牙齿漂白后自由基的减少具有统计学意义。
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引用次数: 0
Evaluation of Dose Dependent Chemopreventive Effect of Diosmin on Induced Oral Squamous Cell Carcinoma 地奥司明对口腔鳞状细胞癌剂量依赖性化学预防作用的评价
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.72736.1352
Menna-tallah Osman, Amany Taha, Nadia A. Radi, Eman AboHager
Purpose: This study aimed to evaluate the chemoprevention efficacy of diosmin on experimentally induced hamster buccal pouch (HBP) squamous cell carcinoma utilizing Ki 67 as a proliferative marker. Material and Methods : Seventy Syrian male hamsters were used as experimental animals, these hamsters were divided into five groups, Group I (negative control): 5 hamsters were left untreated. Group II (DMBA treated group): 20 hamsters were divided equally into two subgroups, painted with 0.5% DMBA where subgroup IIa: for 8 weeks while subgroup IIb: for 14 weeks. Group III: 5 hamsters were orally administrated diosmin 150 mg/kg for 14 weeks. Group IV: 20 hamsters were divided equally into two subgroups, treated with diosmin 100mg/kg and DMBA where subgroup IVa: for 8 weeks while subgroup IVb: for 14 weeks. Group V: 20 hamsters were divided equally into two subgroups, treated with diosmin 150mg/ kg and DMBA where subgroup Va: for 8 weeks while subgroup Vb: for 14 weeks. Results : Comparing all groups and subgroups revealed that the mean values have been arranged with the following descending pattern subgroup IIa, subgroup Va, subgroup IIb, subgroup Vb, subgroup IVb, subgroup IVa, group III and group I. ANOVA test revealed a statistically significant difference between groups. Conclusion : Diosmin 100mg/kg is considered a promising chemopreventive agent in preventing induced HBP squamous cell carcinoma.
目的:以Ki 67为增殖标志物,评价地奥米明对实验性诱导的仓鼠颊袋(HBP)鳞状细胞癌的化学预防作用。材料与方法:选取70只雄性叙利亚仓鼠作为实验动物,将其分为5组,1组(阴性对照):5只仓鼠未经处理。第二组(DMBA处理组):20只仓鼠平均分为两个亚组,分别用0.5% DMBA涂药,其中IIa亚组涂药8周,IIb亚组涂药14周。III组:5只仓鼠口服地臭虫胺150 mg/kg,连续14周。IV组:20只仓鼠平均分为2个亚组,分别给予地奥霉素100mg/kg和DMBA治疗,IVa亚组8周,IVb亚组14周。V组:20只仓鼠平均分为2个亚组,分别给予地奥霉素150mg/ kg和DMBA治疗,其中Va亚组8周,Vb亚组14周。结果:各组和亚组比较,均值按以下降序排列:亚组IIa、亚组Va、亚组IIb、亚组Vb、亚组IVb、亚组IVa、组III、组i。方差分析显示,组间差异有统计学意义。结论:100mg/kg地奥司明是一种很有前景的预防HBP鳞状细胞癌的化学制剂。
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引用次数: 1
Evaluation of Photodynamic Therapy Using Diode Laser with Photosensitizer in Treatment of Chronic Periodontitis 二极管激光联合光敏剂光动力治疗慢性牙周炎的疗效评价
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.58090.1330
Doaa Bhgaat, Hamdy A. Nassar, Naglaa Elkilani, E. Kandeel
Purpose: The current clinical study was conducted to evaluate clinical and biochemical effects of photodynamic therapy in treatment of periodontal disease. Material and methods: The current study was conducted on twenty-eight (28) sites in fourteen (14) patients. A spilt-mouth design was used, sites were randomly selected from each patient, and assigned into two groups:(control group A) fourteen (14) sites received scaling and root planning only, and (test group B): fourteen (14) sites received PDT (Curcumin as photosensitizer agent was irradiated by diode laser beam) and another group without any laser beam in oral cavity, 5 patients received SRP only. Clinical parameters included probing pocket depth (PPD), clinical attachment level (CAL), Plaque index (PI), and gingival index (GI) were used to make clinical evaluation. ELISA analysis of human P.gingivalis IGg was done. Results: Comparison between the groups at baseline, one week and one month was showed significant difference in human P.gingivalis IGg which decreased in test group more than in control group. Conclusion: This clinical study illustrated that SRP in combination with PDT can be effective especially when used as an adjunctive therapy to the mechanical conditioning of the periodontal pockets. Although using Curcumin as natural photosensitizer agent have great effect on enhancement of inflammation in patients with periodontal disease.
目的:评价光动力疗法治疗牙周病的临床及生化效果。材料和方法:本研究在14例患者的28个部位进行。采用口口设计,从每位患者中随机选择部位,分为两组:(对照组)14(14)个部位仅进行刮治和根规划,(试验B组)14(14)个部位进行PDT(姜黄素作为光敏剂,用二极管激光束照射),另一组不进行任何激光照射,5例患者仅进行SRP。采用探查袋深度(PPD)、临床附着水平(CAL)、菌斑指数(PI)、牙龈指数(GI)等临床参数进行临床评价。ELISA法检测人牙龈卟啉单胞菌IGg。结果:组间基线、1周、1月比较,龈假单胞菌IGg水平有显著性差异,试验组下降幅度大于对照组。结论:本临床研究表明,SRP联合PDT治疗牙周袋机械调理的辅助治疗效果显著。虽然使用姜黄素作为天然光敏剂对牙周病患者的炎症有很大的增强作用。
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引用次数: 0
Prevalence of Temporomandibular Joints Disorders in Egyptian Undergraduate Dental Students and The Association Between The Clinical and Radiographic Findings 埃及本科牙科学生颞下颌关节疾病的患病率及其临床和影像学表现之间的关系
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.53600.1323
E. Gomaa, Naglaa El-kilani, Mostafa Abdel-ghany, Noura Abd El-Gawad
Purpose : to assess the prevalence of temporomandibular disorders among dental undergraduate Egyptian students and the association between the clinical fea-tures and radiographic findings. Material and Methods : 281 female dental undergraduate students were randomly selected to participate in the primary screening. In the study screening examination and final diagnosis were done according to a new modified Diagnostic Criteria for TMD (DC/TMD) Axis I which encompass (medi-cal history, primary pain screening questionnaire, signs and symptoms questionnaire and examination protocol). The examination consisted of 11 criteria (pain location, headache location, incisal relationship ,opening pattern ,opening movement ,lateral and protrusive movements ,noises during opening movement ,noises during lateral and protrusive movements, TMJ locking, muscle palpation and TMJ, supplemental muscle pain and palpation, occlusion assessment was added to the criteria Results: the prevalence of TMD was 28.5% among female dental students, the most common reported symptom in a percentage of 89% was pain ,noises were reported in 59.3%f the subjects .The most common subtype of the TMD was muscular pain (mylagia and myofascial pain ) with percentge of 29.2%, 15.4% then arthylagia with 27.7% disc displacement was reported in 13,8 and degenerative reported in 1.5% Conclusions: TMDS is highly prevalent conditions in young population. There is an adequate association between the stress in the university years and the TMDS.
目的:评估埃及牙科本科学生颞下颌疾病的患病率及其临床特征与影像学表现之间的关系。材料与方法:随机抽取281名女口腔本科学生进行初步筛查。在研究中,筛查检查和最终诊断是根据新的修改后的TMD诊断标准(DC/TMD)轴I进行的,其中包括(病史,原发性疼痛筛查问卷,体征和症状问卷和检查方案)。检查包括11项标准(疼痛位置、头痛位置、切牙关系、开口方式、开口运动、侧突运动、开口运动时的噪音、侧突运动时的噪音、TMJ锁定、肌肉触诊和TMJ、补充肌肉疼痛和触诊、咬合评估)。女牙科学生患TMD的比例为28.5%,其中最常见的症状为疼痛(占89%),最常见的症状为噪音(占59.3%),最常见的症状为肌肉疼痛(mylagia和my筋膜疼痛)(占29.2%,15.4%),其次为关节病,椎间盘移位占27.7%(13.8),变性占1.5%(13.8)。在大学期间的压力和tmd之间有充分的联系。
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引用次数: 1
Assessment of Absorbed Radiation Dose of Some Soft and Hard Oral Tissues after Panoramic and Cone Beam CT Radiograph 一些口腔软硬组织在全景和锥束CT造影后吸收辐射剂量的评估
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.48289.1319
Aya Talha, Ossama El-Shall, A. Abaza, Nora Abdelgawad
Purpose: The goal of the this study is to measure absorbed dose of oral tissues such as oral mucosa, salivary gland and bone after exposure to ionizing radiation through panoramic radiograph and cone beam CT. Subjects and Methods: about 50 Egyptian patients were selected in this study who were referred to the department of oral medicine, periodontology, oral diagnosis & radiology, Faculties of Dental Medicine, Al-Azhar university for panoramic or cone beam examination for various dental purposes. Patients were divided into two groups (25 patients each). First Group was subjected to panoramic examination and the second one was subjected to cone beam examination. The systems used were a Planmeca Viso CBCT and Orthopantomogram OP-100 panorama. Assessment of the patient radiation dose was done by thermoluminescent dosimeter (TLD) on the patients. The absorbed radiation dose is then recorded. Data was then analyzed, and statistical calculations were performed. Results: The absorbed radiation skin doses ranged between (130 µGy, 2817.8 µGy) in panorama and (327.99 µGy, 11994 µGy) in CBCT for one exposure. When digital panoramic unit and CBCT were compared, panoramic imaging is 25-37% of CBCT. Parotid gland and oral mucosa absorbed the greatest radiation dose for all panoramic and CBCT patients. CBCT radiation doses are considerably higher than those of digital panoramic unit. Conclusion: The prospective benefits of CBCT in maxillofacial disciplinary are undoubted; but, it is important that their use be totally justified above conventional technique before they are done.
目的:通过全景x线摄影和锥形束CT测量电离辐射照射后口腔黏膜、唾液腺和骨骼等口腔组织的吸收剂量。研究对象和方法:本研究选取约50名埃及患者,这些患者转诊至爱资哈尔大学口腔医学院口腔医学、牙周病科、口腔诊断与放射科,接受各种牙科目的的全景或锥束检查。患者分为两组,每组25例。第一组进行全景检查,第二组进行锥束检查。使用的系统是Planmeca Viso CBCT和OP-100全景正体层析成像。采用热释光剂量计(TLD)对患者进行放射剂量评估。然后记录吸收的辐射剂量。然后对数据进行分析,并进行统计计算。结果:单次暴露时,全景图皮肤吸收辐射剂量为(130µGy, 2817.8µGy), CBCT为(327.99µGy, 11994µGy)。当数字全景单元与CBCT比较时,全景成像是CBCT的25-37%。腮腺和口腔黏膜吸收的辐射剂量最大。CBCT的辐射剂量明显高于数字全景单元。结论:CBCT在颌面部学科中的应用前景不容置疑;但是,重要的是,在它们完成之前,它们的使用要比传统技术完全合理。
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引用次数: 0
Effectiveness of Ginger-Honey Mixture and Rosemary on Remineralization and Prevention of Enamel White Spot Lesions Measured by Color Change 姜蜂蜜和迷迭香对牙釉质白斑再矿化及预防的影响
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.75667.1363
A. Shaker, W. jamil, Doaa El Sharkawy
Purpose : To evaluate the effect of three natural materials (Ginger and honey mixture and rosemary oil) on remineralization and prevention of enamel white spot lesions measured by color change using vita easy shade. Material and methods: The study was carried out in two phases remineralization and prevention phase, sixty four anterior teeth were used for each phase. Teeth were prepared and randomized into four groups according to treatment material (n=8 samples). Group I: control group which receives no treatment. Group II: treated with 1.23% sodium fluoride gel. Group III: treated with ginger-honey mixture. Group IV: treated with rosemary oil. Treatment material was applied for 3 or 6 minutes. In phase (1) color was measured at baseline, after creation of white spot lesion, and after treatment. In phase (2) color was measured at baseline and after treatment. Data were obtained and the differences between groups were estimated by ANOVA test followed by Tukey’s post hoc test. Results: for phase1: There was a statistically significant difference among different groups. The highest mean value of ΔE was for sodium fluoride gel group followed by ginger and honey group at 6 min. For phase2: group III had a better acid resistance effect followed by group II at 6 min. Conclusion: Natural materials can be used for remineralization and prevention of WSL as ginger and honey mixture enhance remineralization following sodium fluoride gel which was more effective. And for acid resistance phase, ginger and honey mixture was more effective.
目的:评价三种天然材料(姜蜂蜜混合物和迷迭香油)对牙釉质白斑病变的再矿化和预防作用。材料与方法:研究分再矿化和预防两阶段进行,每阶段使用64颗前牙。制备牙体,根据治疗材料随机分为4组(n=8个样本)。第一组:不进行任何治疗的对照组。第二组:用1.23%氟化钠凝胶治疗。第三组:姜蜜合剂治疗。第四组:用迷迭香油处理。处理材料敷敷3 ~ 6分钟。在第(1)阶段,在基线、白斑病变产生后和治疗后测量颜色。在第(2)阶段,在基线和治疗后测量颜色。采用方差分析和Tukey事后检验估计组间差异。结果:第一阶段:各组间差异有统计学意义。在第二阶段,第3组抗酸效果较好,第2组抗酸效果次之。结论:天然材料可用于WSL的再矿化和预防,其中生姜和蜂蜜混合物增强氟化钠凝胶后的再矿化效果更有效。而在抗酸期,姜蜜混合液的抗酸效果更好。
{"title":"Effectiveness of Ginger-Honey Mixture and Rosemary on Remineralization and Prevention of Enamel White Spot Lesions Measured by Color Change","authors":"A. Shaker, W. jamil, Doaa El Sharkawy","doi":"10.21608/adjg.2022.75667.1363","DOIUrl":"https://doi.org/10.21608/adjg.2022.75667.1363","url":null,"abstract":"Purpose : To evaluate the effect of three natural materials (Ginger and honey mixture and rosemary oil) on remineralization and prevention of enamel white spot lesions measured by color change using vita easy shade. Material and methods: The study was carried out in two phases remineralization and prevention phase, sixty four anterior teeth were used for each phase. Teeth were prepared and randomized into four groups according to treatment material (n=8 samples). Group I: control group which receives no treatment. Group II: treated with 1.23% sodium fluoride gel. Group III: treated with ginger-honey mixture. Group IV: treated with rosemary oil. Treatment material was applied for 3 or 6 minutes. In phase (1) color was measured at baseline, after creation of white spot lesion, and after treatment. In phase (2) color was measured at baseline and after treatment. Data were obtained and the differences between groups were estimated by ANOVA test followed by Tukey’s post hoc test. Results: for phase1: There was a statistically significant difference among different groups. The highest mean value of ΔE was for sodium fluoride gel group followed by ginger and honey group at 6 min. For phase2: group III had a better acid resistance effect followed by group II at 6 min. Conclusion: Natural materials can be used for remineralization and prevention of WSL as ginger and honey mixture enhance remineralization following sodium fluoride gel which was more effective. And for acid resistance phase, ginger and honey mixture was more effective.","PeriodicalId":7493,"journal":{"name":"Al-Azhar Dental Journal for Girls","volume":"70 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89154052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the Antibacterial Effect of Garlic with Lime on Streptococcus Mutans in Children 大蒜加酸橙对儿童变形链球菌的抑菌效果评价
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.32593.1268
Omnia Moussa, M. Mostfa, salam El-Araby
Purpose : This study was conducted to evaluate the effect of Garlic with lime mouth wash against Chlorhexidine mouthwash on salivary Streptococcus mutans count in a group of Egyptian children. Materials and Methods : A total of 40 Egyptian children were included in this study. Children ages ranged from (6 to 12) years old in a good physical condition. Children were randomly distributed into two groups A & B each of 20 children. In children of group A (using Garlic with lime) each participant was instructed to rinse with 5 ml of garlic with lime mouthwash for one minute. In group B (control group using Chlorhexidine mouthwash 0.1%) each participant was instructed to rinse with 5 ml of CHX for one minute. Results: There was a significant reduction in Streptococcus mutans count in both groups. Conclusion: garlic with lime mouthwash were successful as an antimicrobial agent. It significantly reduced the total bacterial count in the saliva of children when compared to a potent antiseptic like Chlorhexidine.
目的:本研究对一组埃及儿童进行了大蒜酸橙漱口水和氯己定漱口水对唾液变形链球菌计数的影响。材料与方法:本研究共纳入40名埃及儿童。儿童年龄从6岁到12岁不等,身体状况良好。孩子们被随机分为A组和B组,每组20人。在A组儿童中(使用大蒜和酸橙),每个参与者被指示用5毫升大蒜和酸橙漱口水冲洗一分钟。在B组(对照组使用0.1%氯己定漱口水),每位参与者被指示用5ml CHX漱口一分钟。结果:两组患者变形链球菌计数均明显减少。结论:大蒜酸橙漱口水抗菌效果良好。与氯己定等强效杀菌剂相比,它能显著减少儿童唾液中的细菌总数。
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引用次数: 0
Assessment of Treatment Outcomes of Skeletal Class II Malocclusion Using Forsus Fatigue Resistant Device Forsus抗疲劳装置治疗骨骼ⅱ类错颌合的疗效评价
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.58982.1333
Esraa Taha, S. Ibrahim, K. Abdallah, Amany Ibrahim
Purpose: This study was carried out to assess treatment outcomes of Forsus Fatigue Resistant Device (FFRD) used in correction of skeletal Class II malocclusion through lateral cephalometric analysis. Subjects and methods: The study was conducted on ten Class II male patients. Treatment was followed through leveling and alignment till heavy 0.019 x 0.025-inch stainless steel archwires reached, then FFRD was inserted. The FFRD protocol ended when an overcorrected edge to edge incisal relation was obtained. Lateral cephalometric radiographs were taken before therapy and after completion of FRD therapy. The following skeletal parameters were measured from lateral cephalograms: SNA, SNB and ANB angles and linear measurement of total mandibular length, ramus height and corpus length. Measured dental parameters were maxillary and mandibular incisors inclination, amount of mesial movement of mandibular first molar, overjet and overbite. Results: The FFRD had a combined skeletal and dentoalveolar effect. It enhanced the sagittal skeletal relationships through maxillary restriction and mandibular forward repositioning. Dentoalveolar effects were significant in both arches and significant enhancement in overjet and overbite were found. Conclusions: The FFRD is effective in correcting Class II malocclusion through skeletal and dentoalveolar changes.
目的:本研究通过侧位测量分析,评估Forsus抗疲劳装置(FFRD)矫正骨骼ⅱ类错的治疗效果。研究对象和方法:研究对象为10例男性II类患者。随后通过调平和对准治疗,直到达到沉重的0.019 x 0.025英寸不锈钢弓线,然后插入FFRD。当获得过校正的边缘到边缘切缘关系时,FFRD协议结束。在治疗前和完成FRD治疗后分别拍摄侧位头颅x线片。通过侧位头像测量以下骨骼参数:SNA、SNB和ANB角,线性测量下颌总长度、支高和体长。测量的牙齿参数包括上颌和下颌切牙的倾斜度、下颌第一磨牙的近中移动量、覆盖和覆盖咬合。结果:FFRD具有骨骼和牙槽牙联合作用。它通过上颌限制和下颌骨前移来加强矢状骨关系。牙槽效应在两牙弓中均显著,而在覆盖牙合和覆盖牙合中均显著增强。结论:FFRD通过骨骼和牙槽骨的改变矫正II类错牙合是有效的。
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引用次数: 0
Evaluation of Giomer Pit and Fissure Sealant as Compared to Resin Based One 异构体坑缝密封胶与树脂基密封胶的比较
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.25809.1243
Esraa Ibrahim, Samy Albaioumy, M. Mostafa
Purpose : This study aimed to compare the antibacterial effect and microleakage of two types of fissure sealants; Beautisealant and Embrace wet Bond. Materials and Methods : for evaluation of the antibacterial effect, 30 saliva samples were obtained and were sent to microbiology laboratory where streptococcus mutans were isolated and the antibacterial effect of both materials was detected by the agar diffusion method. For microleakage test 30 extracted sound permanent molars were obtained. Two groups were formed (Group A &Group B) 15 teeth each. Teeth in group A were sealed with Embrace wet bond while Beautisealant was used to seal teeth in group B. Dye penetration was performed then specimens were put for 24 hours in 2% buffered methylene blue dye. Resin blocks were prepared then longitudinally sectioned buccolingually, The specimens were assessed for dye penetration with digital microscope. Results : For antibacterial effect it was found that Embrace wet Bond recorded higher bacterial inhibition zone than Beautisealant. Mann-Whitney-test (P=<0.0001< 0.05) was used to determine the difference between both groups and showed statistical significance. For the microleakage test it was found that Beautisealant recorded higher value of leakage than Embrace wet Bond. The difference between both groups showed statistical non-significance as determined by Mann-Whitney-test (P=0.4> 0.05). Conclusion : Embrace wet bond pit and fissure sealant could be better option than Beautisealant regarding the antibacterial effect however no difference was noticed between them regarding the microleakage.
目的:比较两种裂隙封闭剂的抗菌效果和微渗漏情况;美丽密封胶和拥抱湿粘合。材料与方法:为评价抗菌效果,取30份唾液样本送微生物实验室分离变形链球菌,采用琼脂扩散法检测两种材料的抗菌效果。进行微漏试验,取出30颗正常恒磨牙。两组(A组和B组)各15颗牙。A组用Embrace湿粘接剂封闭牙体,b组用Beautisealant封闭牙体。染色渗透后用2%缓冲亚甲蓝染料浸泡24小时。制备树脂块,经口腔纵向切片,用数码显微镜观察染色渗透情况。结果:在抗菌效果方面,Embrace wet Bond比Beautisealant有更高的抑菌区。mann - whitney检验(P= 0.05)。结论:在抗菌效果上,采用拥抱湿键窝和裂隙密封剂优于美妆密封剂,但在微渗漏方面两者无显著差异。
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引用次数: 0
Comparative Study on The Remineralizing and Antimicrobial Effect of Naturally and Synthetically based Agents 天然与合成药物再矿化及抗菌效果的比较研究
Pub Date : 2022-07-01 DOI: 10.21608/adjg.2022.103768.1446
M. Sadik, W. jamil, Asmaa Mosleh
Purpose : This study aimed to evaluate and compare the remineralizing and antimicrobial effect of chitosan-based agent and Flavonoid. Materials and methods: This study was carried out in two phases; remineralization phase and antimicrobial phase. Ninety (90) bovine mandibular incisors were used in the study with forty-five (45) teeth in each phase. Teeth were prepared and randomized into three groups according to treatment material (n=15 samples) as follows; group I; control group (being immersed in artificial saliva), group II; treated with flavonoid and finally group III; treated with chitosan-based agent. Each agent was applied for 10 minutes six times/day. In phase (1) remineralizing effect was measured through recording radiodenisty (by using digital radiography) at baseline, after creation of lesion and after treatment. In phase (2) antimicrobial effect was measured (by using colony forming unit test and lactic acid production test) at baseline and after treatment. Data were obtained and the differences between groups were estimated by ANOVA test followed by Tukey’s post hoc test. Results : Statistical analysis by ANOVA test revealed that there was high statistically significant difference between the study groups. Dentin blocks treated by chitosan-based agent showed greater remineralizing effect than flavonoid. Dentin blocks with 1,4 and 7 days biofilms treated with Flavonoids showed greater inhibition of Lactic acid production and Colony forming unit. Conclusion :- Chitosan-based agent can be used as remineralizing agent while flavonoid can be used as antimicrobial agent against S.mutans by inhibiting colony forming unit and lactic acid production..
目的:评价和比较壳聚糖基药剂和类黄酮的再矿化和抗菌效果。材料与方法:本研究分两期进行;再矿化阶段和抗菌阶段。在研究中使用了90(90)个牛下颌门牙,每个阶段45(45)个牙齿。制备牙体,根据治疗材料随机分为3组(n=15个样本):组我;对照组(浸泡人工唾液),II组;黄酮处理组和ⅲ组;用壳聚糖基药剂处理。每剂应用10分钟/天6次。在第(1)阶段,通过记录基线、病变产生后和治疗后的放射密度(使用数字放射照相)来测量再矿化效果。在第(2)阶段,采用菌落形成单元试验和乳酸生成试验测定基线和治疗后的抗菌效果。采用方差分析和Tukey事后检验估计组间差异。结果:经方差分析(ANOVA),各研究组间差异有显著统计学意义。壳聚糖基牙本质阻断剂处理后的再矿化效果优于类黄酮。黄酮生物膜处理1、4和7 d的牙本质阻滞对乳酸生成和菌落形成单位的抑制作用更大。结论:壳聚糖类药物可作为再矿化剂,而黄酮类药物可通过抑制菌落形成单元和乳酸生成来作为抗变形链球菌的抗菌药物。
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引用次数: 0
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Al-Azhar Dental Journal for Girls
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