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The Biological Activity of Some Extracted Proteins from Bacillus spp. Isolated from Soil Against Pathogenic Bacteria 土壤中芽孢杆菌提取蛋白对致病菌的生物活性研究
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.685
H. A. Ibrahim, N. H. Zaki
Forty soil samples were collected from different regions in Baghdad city. Among 52 isolate, only 38 isolates (73.1 %) belong to Bacillus spp. All bacterial isolates have been submitted to the cultural, microscopical, biochemical examination and VITEK 2 System, Bacillus cereus was predominantly found 21 (55.3%) followed by Bacillus subtilis 11 (28.9%), then Bacillus amyloliquefaciens was 6 (15.8%). Bacillus cereus (B1) and Bacillus subtilis (B16) isolates were with the highest level with the antibacterial activity when selected. Extracellular proteins were extracted and purified with ammonium sulfate (80%) saturation, then submitted to Sepharose-6B gel filtration column. SDS-polyacrylamide gel electrophoresis was used to determine the molecular weights of proteins, and one band appeared to each of Bacillus cereus and Bacillus subtilis with molecular weight 68 and 50 KDa respectively. The results revealed that higher effect of antibacterial activity on Gram positive bacteria, and moderate on Gram negative bacteria except P.aeruginosa with no effect. The antibiofilm effect of the extract found higher effective against S.pyogens, moderate effect against each of (S.aureus, E.coli, S. marcescens, K. spp) and no effect against P.aeruginosa. The results of cytotoxicity showed that the purified extract was a higher cytotoxic effect on breast cancer MCF7 cells at 1000µg/ml concentration, while the crude extract had a higher cytotoxic effect on breast cancer MCF7 cells at 100µg/ml concentration.
从巴格达市的不同地区收集了40个土壤样本。52株分离物中,芽孢杆菌属38株(73.1%),经培养、镜检、生化及VITEK 2系统检测,蜡样芽孢杆菌最多21株(55.3%),其次为枯草芽孢杆菌11株(28.9%),解淀粉芽孢杆菌6株(15.8%)。蜡样芽孢杆菌(B1)和枯草芽孢杆菌(B16)菌株的抑菌活性最高。提取细胞外蛋白,硫酸铵(80%)饱和纯化,经Sepharose-6B凝胶过滤柱。用sds -聚丙烯酰胺凝胶电泳法测定蛋白质分子量,蜡样芽孢杆菌和枯草芽孢杆菌各有一条条带,分子量分别为68和50 KDa。结果表明,对革兰氏阳性菌有较高的抑菌活性,对革兰氏阴性菌除铜绿假单胞菌外,抑菌活性一般,无效果。该提取物对脓毒杆菌的抗菌效果较好,对金黄色葡萄球菌、大肠杆菌、粘质葡萄球菌、K. spp均有中等效果,对铜绿假单胞菌无效果。细胞毒性实验结果显示,纯化提取物在1000µg/ml浓度下对乳腺癌MCF7细胞具有较高的细胞毒作用,而粗提取物在100µg/ml浓度下对乳腺癌MCF7细胞具有较高的细胞毒作用。
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引用次数: 0
Strongly Weakly Supplement Extending Modules 强弱补充扩展模块
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.682
Aya Adnan Musa, S. Al-Saadi
In this paper, a class of modules which are proper strong concept of weakly supplement extending modules will be introduced and studied. We call a module M is strongly weakly supplement extending, if each submodule of M is essential in fully invariant weakly supplement submodule in M. Many characterizations of strongly weakly supplement extending modules are obtained. We show that M is strongly weakly supplement extending module if and only if every closed submodule of M is fully invariant weakly supplement submodule in M. Also we study the relation among this concept and other known concepts of modules. Moreover, we give some conditions that of strongly weakly supplement extending modules is closed under direct sum property is strongly weakly supplement extending.
本文引入并研究了一类弱互补扩展模的强概念。我们称模M为强弱互补扩展,如果M的每一个子模在M中的完全不变弱互补子模中是本质的,则得到了强弱互补扩展模的许多表征。证明M是强弱补扩展模当且仅当M的所有闭子模都是M中的完全不变弱补子模,并研究了该概念与其他已知模概念的关系。此外,还给出了强弱补扩展模在直和性质下是强弱补扩展的一些条件。
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引用次数: 0
Supra ω-separation axioms 超ω分离公理
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.691
Nadia Kadum Humadi, H. J. Ali
The purpose of this paper is to introduce new types of supra separation axioms by using supra ω-open sets and supra N-open sets in the supra spaces and illustrate the relation between them, and to introduce new forms of supra continuous functions, supra perfectly continuous functions and supra homeomorphism functions. الخلاصـة الغرض من هذا البحث هو لتقديم انواع جديدة من بديهيات الفصل الفوقية بأستخدام المجموعات المفتوحة-ω الفوقية والمجموعات المفتوحة -N الفوقية في الفضاءات الفوقية وتوضيح العلاقة بينهم, ولتقديم صيغ جديدة من الدوال المستمرة الفوقية, الدوال المستمرة الفوقية التامة والدوال التشاكلية الفوقية.
对扩大扩大扩大到扩大到扩大到扩大到扩大到其他地区,并扩大到其他地区,扩大到其他地区,扩大到其他地区,并扩大到其他地区。الخلاصـة本文的目的是提供品种,公理元章利用公开-ω公开元集团和n元空间元数据和澄清,提供新版本的关系全面持续的函数调用元元,连续函数调用والدوالالتشاكلية元。
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引用次数: 0
ON JORDAN IDEAL IN PRIME AND SEMIPRIME INVERSE SEMIRINGS WITH CENTRALIZER 带扶正器的素数和半素数逆半环的Jordan理想
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.710
Rawnaq Khaleel Ibraheem, A. Majeed
In this paper we recall the definition of centralizer on inverse semiring. Also introduce the definition of Jordan ideal and Lie ideal. Some results of M.A.Joso Vukman on centralizers on semiprime rings are generalized here to inverse semirings.
本文回顾了逆半环上扶正器的定义。同时介绍了乔丹理想和李理想的定义。本文将M.A.Joso Vukman关于半素环上中心点的一些结果推广到逆半环上。
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引用次数: 1
On mω ̂-light functions 在m³光函数上
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.718
Nadia Kadum Humadi, H. J. Ali
In this work we introduced new types of -light functions namely -light functions, also we introduced -disconnected, and -totally disconnected spaces. Some examples, facts, and theorems have been given to support our work.
在这项工作中,我们引入了新型的-光函数即-光函数,我们还引入了-不连通空间和-完全不连通空间。给出了一些例子、事实和定理来支持我们的工作。
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引用次数: 1
Sero-prevalence of toxoplasmosis and Role of Cathepsin L-like and Cathepsin B-like Genes as Risk Factors for Abnormal Pregnancy Outcome 弓形虫病的血清患病率及组织蛋白酶l样和组织蛋白酶b样基因在异常妊娠结局中的作用
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.720
noor meran omer, H. M. Alsakee
Background and objectives: Toxoplasma gondii ( T. gondii ) is a ubiquitous apicomplexan parasite. As an obligate intracellular parasite, T. gondii must invade host cells to survive and replicate. Five cathepsin proteases are encoded in the genome of T . gondii, cathepsin L like protein, cathepsin B like protein, and three cathepsin C like proteins. The present study was aimed to investigate the prevalence of toxoplasmosis among women in Erbil, and to study the role of cathepsin B and cathepsin L genes in the pathogenesis of toxoplasmosis as well as their role as risk factors for abnormal pregnancy outcome. Methods: This is a cross sectional study was carried out in Erbil from October 2018 to March 2019.  A total of 230 women at their reproductive age and who attended Maternity Teaching Hospital and Nazdar Bamarni primary Health Center were enrolled. Anti- toxoplasma IgG and IgM antibodies were detected by cobas 6000. Toxoplasma cathepsin B and cathepsin L – like genes were selected to be targets in PCR. Results: Anti-toxoplasma IgG and IgM were seropositive in 105 (45.7%) and 18 (7.8%) women, respectively. Of those women who were seropositive for toxoplasmosis, only 15(6.5%) of them were carrying both anti-toxoplasma IgG and IgM. No significant association of toxoplasmosis and educational level, socioeconomic level, age, history of abortion, abnormal baby birth weight were observed. PCR targeting cathepsin L was more sensitive to be used in the diagnosis of toxoplasmosis. Conclusion: Sero-prevalence of toxoplasmosis is relatively high in Erbil and cathepsin L gene is an efficient target for PCR and could be used as risk factor for abnormal pregnancy outcome.
背景与目的:刚地弓形虫是一种普遍存在的顶复合体寄生虫。作为一种专性细胞内寄生虫,弓形虫必须侵入宿主细胞才能生存和复制。五种组织蛋白酶被编码在T的基因组中。弓形虫,组织蛋白酶L样蛋白,组织蛋白酶B样蛋白,以及三种组织蛋白酶C样蛋白。本研究旨在调查埃尔比勒地区妇女弓形虫病的流行情况,研究组织蛋白酶B和组织蛋白酶L基因在弓形虫病发病中的作用及其作为异常妊娠结局危险因素的作用。方法:这是一项横断面研究,于2018年10月至2019年3月在埃尔比勒进行。共有230名育龄妇女在妇产科教学医院和纳兹达尔·巴马尼初级保健中心就诊。用cobas 6000检测抗弓形虫IgG和IgM抗体。选取弓形虫组织蛋白酶B和组织蛋白酶L样基因作为PCR的靶基因。结果:女性血清抗弓形虫IgG阳性105例(45.7%),IgM阳性18例(7.8%)。在弓形虫血清检测阳性的妇女中,仅有15人(6.5%)同时携带抗弓形虫IgG和IgM抗体。弓形虫病与受教育程度、社会经济水平、年龄、流产史、新生儿异常体重无显著相关性。以组织蛋白酶L为靶点的PCR对弓形虫病的诊断更为敏感。结论:埃尔比勒地区弓形虫病血清患病率较高,组织蛋白酶L基因是PCR检测的有效靶点,可作为异常妊娠结局的危险因素。
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引用次数: 0
Phenotypic Characterization of Extended-Spectrum Beta-Lactamases and Metallo-Beta-Lactamase of Multi Drug Resistant Acinetobacter baumannii Causing Nosocomial Infections in Erbil City 埃尔比勒市多重耐药鲍曼不动杆菌引起医院感染的广谱β -内酰胺酶和金属β -内酰胺酶表型特征
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.671
S. Smail, Kamal I. Al-Otrachi
Background: Resistance to broad‑spectrum beta‑lactams, mediated by extended‑spectrum beta‑lactamases, and metallo‑beta‑lactamase enzymes, is an increasing problem worldwide. The main aim is to study phenotypic characterization of extended‑spectrum beta‑lactamases and metallo‑beta‑lactamase multidrug resistant Acinetobacter baumannii in Erbil City. Materials and Methods: A total of 112 Acinetobacter baumannii isolations were collected from patients of all age groups from clinical specimens sputum, blood, pus, wound swab, urine and body fluids (Pleural fluid and cerebrospinal fluid) collected from different medical wards and intensive care unit departments of hospitals in Erbil City for a period of one year from march 2018--march 2019. Isolates were tested for the presence of extended‑spectrum beta‑lactamases and metallo‑beta‑lactamase. Detection of extended‑spectrum beta‑lactamases was done by the combined disk diffusion method according to Clinical and Laboratory Standards Institute guidelines, while metallo‑beta‑lactamase was detected by meropenem and imipenem combined with ethylenediaminetetraacetic acid disk method. Results: 25% (28) Acinetobacter baumannii isolates were positive for extended‑spectrum beta‑lactamases, while 100 % (112) were metallo‑beta‑lactamase producers. Conclusion: Acinetobacter baumannii is becoming a global medical challenge due to the emergence of multi-drug resistance. Newer beta lactamase is a matter of concern as they are developing rapidly and lead to treatment failure. Carbapenems are known to be effective therapeutic agents for Acinetobacter baumannii infections and its resistance limits the use to polymyxins and colistin. Several new medicines are still in research and combination of drug therapy is being currently used in the hospitals together with ours to treat multidrug resistant Acinetobacter baumannii infections.
背景:由广谱β -内酰胺酶和金属β -内酰胺酶介导的广谱β -内酰胺耐药在世界范围内是一个日益严重的问题。主要目的是研究埃尔比勒市广谱β -内酰胺酶和金属β -内酰胺酶多重耐药鲍曼不动杆菌的表型特征。材料与方法:2018年3月- 2019年3月,从埃尔比勒市医院不同病房和重症监护病房收集临床标本,包括痰、血、脓、伤口拭子、尿液和体液(胸膜液和脑脊液),从各年龄组患者中分离出鲍曼不动杆菌112株。检测分离物是否存在广谱β -内酰胺酶和金属β -内酰胺酶。扩展谱β -内酰胺酶检测采用联合圆盘扩散法,按照临床与实验室标准协会指南,金属β -内酰胺酶检测采用美罗培南、亚胺培南联合乙二胺四乙酸圆盘法。结果:25%(28株)鲍曼不动杆菌对广谱β -内酰胺酶呈阳性,100%(112株)为金属β -内酰胺酶产生菌。结论:鲍曼不动杆菌因出现多重耐药而成为全球性的医学挑战。较新的β内酰胺酶是一个值得关注的问题,因为它们发展迅速,导致治疗失败。碳青霉烯类已知是鲍曼不动杆菌感染的有效治疗剂,其耐药性限制了多粘菌素和粘菌素的使用。一些新药仍在研究中,目前正在与我们的医院一起使用药物联合治疗来治疗多重耐药鲍曼不动杆菌感染。
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引用次数: 3
Statistical Distribution of Rainfall in Kurdistan-Iraq Region 库尔德斯坦-伊拉克地区降雨的统计分布
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.662
M. Fawzee, S. Salh, Slahaddin A. Ahmed
Study the statistical distribution for rainfall is important to know the behaviour of the rainfall series and to know the most frequently rainfall amount in each month. Five statistical distribution were applied on Sulaimani, Erbil and Duhok rainfall series for the period (1941-2017) except Duhok (1944-2017). These distributions were Gamma(3P), Weibul(3P), Earlang (3P), Normal and General extreme value. Kolmogrove-Semirnov, Anderson-Darling and Chi-Square goodness of fit test were used to know the best fit distribution from these five distributions.
研究雨量的统计分布,对于了解雨量序列的变化,以及了解每个月最频繁的雨量,是很重要的。对苏莱曼尼、埃尔比勒和杜霍克地区除杜霍克地区(1944-2017年)外的1941-2017年降水序列进行了5次统计分布。这些分布分别是Gamma(3P)、Weibul(3P)、Earlang (3P)、Normal和General极值。使用Kolmogrove-Semirnov、Anderson-Darling和卡方拟合优度检验从这五个分布中得到最佳拟合分布。
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引用次数: 3
MANIFESTATIONS OF CLIMATE CHANGE IN BAGHDAD AREA 巴格达地区气候变化的表现
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.657
H. J. Abdulla
This investigation has been conducted to study the manifestations of climate change in Baghdad area. The climate in Baghdad is recognized as hottest in late June , all July and early August The average surface temperatures of the , asphalt depicted warmest surface temperatures, followed by the pavers, concrete, grass and Air. These were were 64.1, 62.0 , 57.8, 49.7 and 49 C°respectively.. The vegetation is the lowest temperatures of all materials. The comparison between air temperature and other materials. asphalt , pavers, and concrete were highly significant at the 0.01 level, The harsh climate ( climate change ) has effected the common plant that grow in Baghdad such as Bitter orange ( Citrus aurantium ) , the date palm (Phoenix dactylifera.) , Chinese thuja shrub ( Platycladus orientalis) and also has effected land . Iraq climate was affected by global climatic change.
这次调查是为了研究巴格达地区气候变化的表现。巴格达的气候在6月下旬、整个7月和8月初被认为是最热的。沥青路面的平均表面温度最高,其次是铺路石、混凝土、草地和空气。分别为64.1、62.0、57.8、49.7、49℃。植物是所有材料中温度最低的。空气温度与其他材料的比较。恶劣的气候(气候变化)影响了生长在巴格达的常见植物,如苦橙(Citrus aurantium),枣椰树(Phoenix dactylifera.),中国土树灌木(Platycladus orientalis),也影响了土地。伊拉克气候受到全球气候变化的影响。
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引用次数: 2
OPACITY INFLUENCED INCONSTANT METHOD FOR 3D HOLOGRAPHIC PYRAMID RENDERING 不透明度影响三维全息金字塔绘制的非恒定方法
Pub Date : 2020-01-15 DOI: 10.23851/mjs.v30i4.597
Sarah َQahtan Mohammed Salih, P. S. Sulaiman, Abdul Sattar Arif Khammas, R. Mahmod, R. Rahmat
The rapid growth of computer graphics in human daily life has inspired researchers to maximize image understanding and discover new methods for visualizing 3D objects. However, the image quality is constrained by the depth cue limitations of 3D objects produced by 3D displays. The advent of the holographic hologram pyramid display offers better image quality due to its ability to visualize 3D data with satisfactory depth. In this paper, we present a new visualization method named the “opacity influenced inconstant method” that exploits the custom surface rendering technique to enhance understanding of massive 3D objects for a hologram pyramid display. An algorithm is developed by adding an opacity variable and manipulating the intensity and position of the other variables to enhance the depth cues of a 3D object. The opacity value has the ability to show the inner structure of an object to be visualized if it is available. Our results show significant enhancement on the depth cues of the 3D object that presents as a hologram floating inside a transparent pyramid. The results of a survey conducted on computer graphics students shows that the quality of the hologram was preferred when it was compared to the previous method. In addition, the depth cues of the 3D object were reported to be enhanced by the opacity influenced inconstant method when compared to the previous study and standard shading.
计算机图形学在人类日常生活中的快速发展激发了研究人员最大限度地理解图像并发现可视化3D对象的新方法。然而,图像质量受到3D显示器产生的3D物体的深度提示限制的限制。由于能够以令人满意的深度可视化3D数据,全息全息图金字塔显示器的出现提供了更好的图像质量。在本文中,我们提出了一种新的可视化方法,称为“不透明度影响非恒定方法”,该方法利用自定义表面渲染技术来增强对全息图金字塔显示的大量3D物体的理解。通过增加一个不透明度变量和操纵其他变量的强度和位置来增强3D对象的深度线索,开发了一种算法。不透明度值能够显示要可视化的对象的内部结构,如果它可用的话。我们的研究结果显示,3D物体的深度线索显著增强,呈现为漂浮在透明金字塔内的全息图。一项针对计算机图形学学生的调查结果表明,与以前的方法相比,全息图的质量更佳。此外,与以往的研究和标准着色相比,采用不透明度影响的非恒定方法可以增强三维物体的深度线索。
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引用次数: 1
期刊
Al-Mustansiriyah Journal of Sciences
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