American Journal of Reproductive Immunology最新文献_第7页

American Society of Reproduction Immunology Guideline on Recurrent Pregnancy Loss: Between Evidence-Based Practice and Excessive Medicalization 美国生殖免疫学学会关于复发性妊娠丢失的指南：在循证实践和过度医学化之间

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-09-04 DOI: 10.1111/aji.70152

Melania Maria Ramos Amorim, Anna Catharina Carneiro da Cunha, Giordana Campos Braga, Lucas Félix Marinho Neves, Marina Amorim Albuquerque, Alexandre Delgado, Edson Borges de Souza, Leila Katz

引用次数: 0

Chemokines and Their Association With Symptom Severity in Women With Endometriosis 子宫内膜异位症患者的趋化因子及其与症状严重程度的关系

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-09-04 DOI: 10.1111/aji.70156

Anton Sommar, Pinar Yalcin Bahat, Ipek Yildiz Özaydin, Eray Metin Güler, Marie Bixo, Torbjörn Bäckström, Engin Oral, Sahruh Turkmen

Problem

Various chemokines have been linked to endometriosis. Notably, chemokines such as CCL2, CXCL8, and CXCL1 have also been shown to promote nociception. In this study, we investigated whether increased serum concentrations and endometrial expression of chemokines (specifically CCL2, CXCL8, and CXCL1) are associated with heightened severity of pain symptoms in women with endometriosis.

Method of Study

The study included women with endometriosis (with [n = 27] and without [n = 24] hormonal treatment) as well as healthy controls (n = 22). All participants underwent blood sampling and an endometrial biopsy during the secretory phase of the menstrual cycle. Symptom severity in the patient group was assessed using the pain dimension of the Endometriosis Health Profile 30 (EHP-30) and a visual analog scale (VAS) for pain.

Results

Serum levels of CCL2 and CXCL1, as well as endometrial expression of CXCL8, were lower in women with endometriosis compared to controls. Furthermore, increased serum levels of CCL2, CXCL8, and CXCL1 were associated with higher EHP-30 pain domain scores in women with endometriosis. Similarly, elevated endometrial expression of CXCL8 and CXCL1 correlated with higher VAS scores. Notably, when the patient group was stratified based on ongoing hormonal treatment, CXCL1 emerged as the most promising target, with both increased serum concentration and endometrial expression consistently being associated with greater symptom severity.

Conclusions

Our results suggest that chemokines, particularly CXCL1, are associated with greater pain severity and reduced quality of life in women with endometriosis. However, these correlations do not establish causality and should be interpreted with caution.

各种趋化因子与子宫内膜异位症有关。值得注意的是，趋化因子如CCL2、CXCL8和CXCL1也被证明可以促进伤害感受。在这项研究中，我们调查了血清浓度和子宫内膜趋化因子（特别是CCL2、CXCL8和CXCL1）表达的增加是否与子宫内膜异位症女性疼痛症状的加重有关。研究包括子宫内膜异位症患者（接受激素治疗[n = 27]和未接受激素治疗[n = 24]）以及健康对照组（n = 22）。所有的参与者都在月经周期的分泌期接受了血液采样和子宫内膜活检。使用子宫内膜异位症健康概况30 （EHP-30）的疼痛维度和视觉模拟疼痛量表（VAS）评估患者组的症状严重程度。结果与对照组相比，子宫内膜异位症患者血清CCL2、CXCL1水平及子宫内膜CXCL8表达均较低。此外，血清CCL2、CXCL8和CXCL1水平升高与子宫内膜异位症患者EHP-30疼痛域评分升高相关。同样，子宫内膜CXCL8和CXCL1表达升高与VAS评分升高相关。值得注意的是，当患者组根据正在进行的激素治疗进行分层时，CXCL1成为最有希望的靶点，血清浓度和子宫内膜表达的增加始终与更严重的症状相关。我们的研究结果表明，趋化因子，特别是CXCL1，与子宫内膜异位症患者的疼痛严重程度和生活质量下降有关。然而，这些相关性并不能建立因果关系，应谨慎解释。

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引用次数: 0

Expression of MHC Class I Molecules (HLA-A, -B, -C, -E, -F, -G, and -J) Decreases From Early to Late Stage in Ovarian Cancer 从卵巢癌早期到晚期，MHC I类分子（HLA-A、-B、-C、-E、-F、-G和-J）的表达减少

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-09-04 DOI: 10.1111/aji.70154

Caglar Berkel

Problem

Interferon-ε (IFNε), which is highly abundant in the epithelium of the female reproductive tract (FRT), is a recently identified tumor suppressor for ovarian cancer. IFNε induces the expression of certain HLA class I family members in HGSOC (high-grade serous ovarian cancer), and its expression is lost during ovarian tumorigenesis. However, tumor stage–dependent expression of HLA class I family members in ovarian cancer has not been previously studied.

Method of Study

Data analysis and visualization were performed using various gene expression and transcriptomics datasets in the R statistical programming environment.

Results

We found that the expression of HLA-A, -B, -C, -E, -F, -G, and -J is lower in late stage ovarian tumors compared to early-stage tumors. The total expression of HLA class I family members decreases with age in ovarian cancer. Furthermore, we showed that the expression of some IFN-regulated genes, which were shown to be upregulated by IFNε, decreases from early to late stage in ovarian cancer, in parallel to the loss of IFNε expression in ovarian tumorigenesis and possibly in tumor progression. We also found that breast tumors (another hormonally driven cancer) with positive progesterone receptor status have lower IFNε mRNA expression compared to those with negative PR status. Besides, we reported that breast tumors with positive estrogen receptor (ER) status have lower expression of IFNε compared to those with negative ER status.

Conclusions

Combined, this study points that the decrease in the expression of IFNε, HLAs, or some other IFNε-regulated genes during ovarian cancer progression might contribute to worse prognosis in advanced disease.

问题干扰素-ε (IFNε)是最近发现的一种卵巢癌抑制因子，在女性生殖道上皮（FRT）中含量丰富。IFNε在HGSOC（高级别浆液性卵巢癌）中诱导某些HLA I类家族成员的表达，其表达在卵巢肿瘤发生过程中丢失。然而，HLA I类家族成员在卵巢癌中的肿瘤分期依赖性表达尚未被研究。研究方法在R统计编程环境中使用各种基因表达和转录组学数据集进行数据分析和可视化。结果HLA-A、-B、-C、-E、-F、-G和-J在晚期卵巢肿瘤中的表达低于早期肿瘤。HLA I类家族成员在卵巢癌中的总表达量随年龄的增长而降低。此外，我们发现一些ifn调节基因的表达从卵巢癌早期到晚期下降，这些基因被IFNε上调，这与IFNε在卵巢肿瘤发生和肿瘤进展过程中的表达下降是平行的。我们还发现孕酮受体阳性的乳腺肿瘤（另一种激素驱动的癌症）与PR状态阴性的肿瘤相比，IFNε mRNA表达较低。此外，我们报道了雌激素受体（ER）阳性的乳腺肿瘤与ER阴性的肿瘤相比，IFNε的表达较低。综上所述，本研究指出，在卵巢癌进展过程中，IFNε、hla或其他IFNε调控基因的表达减少可能导致晚期疾病预后恶化。

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引用次数: 0

Comments on “Effect of COVID-19 Infection During Pregnancy on the Plasma/Extracellular Vesicles Proinflammatory Cytokine Profile” 对《妊娠期COVID-19感染对血浆/细胞外囊泡促炎细胞因子谱的影响》的评论

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-09-02 DOI: 10.1111/aji.70149

Renu Sah, Ankita Mathur, Venkata Dileep Kumar Veldi

引用次数: 0

Comment on “Outcomes of Ultimpro Testing Implementation for Patients With Unexplained Recurrent Implantation Failure: A Single-Center Retrospective Cohort Study” 对“Ultimpro检测用于不明原因复发性植入失败患者的疗效：一项单中心回顾性队列研究”的评论

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-08-28 DOI: 10.1111/aji.70146

Ranjana Sah, Rachana Mehta

引用次数: 0

Dynamics of Cytokines and Chemokines During the Peripartum Period in People Living With Human Immunodeficiency Virus 人类免疫缺陷病毒感染者围生期细胞因子和趋化因子的动态变化

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-08-22 DOI: 10.1111/aji.70147

Jacqueline Corry, Natalia Zotova, Martine Tabala, Christina K. Cotrone, Fidéle Lumande Kasindi, Bienvenu Lebwaze Massamba, Pelagie Babakazo, Namal P. M. Liyanage, Nicholas T. Funderburg, Marcel Yotebieng, Jesse J. Kwiek

Problem

Pregnancy requires a precisely regulated immune response to support fetal development and minimize complications. Human immunodeficiency virus (HIV) infection induces a chronic inflammatory state and is associated with adverse pregnancy outcomes. In this observational cohort of pregnant people living with HIV in the Democratic Republic of the Congo (DRC), we sought to gain a deeper understanding of peripartum changes in cytokines, chemokines and soluble factors (collectively termed immune factors).

Method of Study

Pregnant individuals living with HIV were enrolled during their second or third trimester in Kinshasa, DRC, between October 2020 and May 2021. Peripheral blood samples were collected at: enrollment (second or third trimester), 1–3 days postdelivery and postpartum. Concentrations of 45 immune factors were measured using LegendPlex and ELISAs.

Results

Most chemokines decreased significantly from enrollment to postdelivery, followed by a rebound in the postpartum period. Meanwhile, concentrations of myoglobin, serum amyloid A-1 protein (SAA), and interleukin 6 (IL-6), increased from enrollment to postdelivery, followed by a decrease postpartum. Finally, protein S100-A8/protein S100-A9 (S100A8/A9) and insulin-like growth factor-binding protein 4 (IGFBP4) consistently increased from enrollment through postpartum.

Conclusions

The postdelivery decline in chemokines observed in this study has not previously been reported. This shift may result from two mechanisms: greater-than-expected placental chemokine production or the degradation of key signaling molecules during parturition and early uterine involution. Additionally, the rise in proinflammatory markers from enrollment to postdelivery suggests a persistent inflammatory state, either unaffected by fetal delivery or worsened by tissue damage in the gestational parent.

怀孕需要精确调节的免疫反应来支持胎儿发育并减少并发症。人类免疫缺陷病毒（HIV）感染诱导慢性炎症状态，并与不良妊娠结局相关。在刚果民主共和国（DRC）感染艾滋病毒的孕妇观察队列中，我们试图更深入地了解围产期细胞因子、趋化因子和可溶性因子（统称为免疫因子）的变化。研究方法2020年10月至2021年5月期间，在刚果民主共和国金沙萨招募了妊娠中期或晚期感染艾滋病毒的孕妇。在入组（妊娠中期或晚期）、分娩后1-3天和产后采集外周血样本。采用LegendPlex和elisa检测45种免疫因子的浓度。结果大多数趋化因子从入组到产后显著下降，并在产后出现反弹。同时，肌红蛋白、血清淀粉样蛋白a -1 （SAA）和白细胞介素6 （IL-6）的浓度从入组到分娩后升高，产后下降。最后，从入组到产后，S100-A8/ S100-A9蛋白（S100A8/A9）和胰岛素样生长因子结合蛋白4 （IGFBP4）持续升高。结论：本研究中观察到的分娩后趋化因子下降未见报道。这种转变可能是由两种机制引起的：分娩和子宫复旧早期胎盘趋化因子的产生或关键信号分子的降解。此外，从入组到分娩后，促炎标志物的上升表明持续的炎症状态，要么不受胎儿分娩的影响，要么因妊娠父母的组织损伤而恶化。

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引用次数: 0

Menstrual Effluent Derived Immune Cell Composition Is Distinct in Women Using Contraceptives: A Pilot Study 月经流出物衍生的免疫细胞组成在使用避孕药的妇女中是不同的：一项试点研究

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-08-22 DOI: 10.1111/aji.70145

Aysel Gurbanova, Anne Stoverink, Imke M. B. van Wandeloo, Annemiek Nap, Nicole M. de Roos, Marien I. de Jonge, Janneke S. Hoogstad - van Evert, Renate G. van der Molen

Problem

Menstrual effluent (ME) is widely used to study immune-related reproductive disorders. However, women using contraceptives are often excluded from such studies due to limited knowledge of their impact on the endometrial immune cell composition. This gap in knowledge restricts our ability to include women using contraceptives in the scientific investigations and account for potential immunological variations associated with contraceptive use. Therefore, we aim to investigate whether contraceptive use is associated with differences in the uterine immune system.

Method of Study

ME was collected from women using combined oral contraceptives (COC, n = 5), a copper intrauterine device (IUD, n = 3), or no contraceptives (n = 5). Immune cells were isolated from ME and analyzed using flow-cytometry. Data were analyzed using an unpaired t-test, followed by multiple testing correction using the false discovery rate (FDR) method.

Results

Women using COCs exhibited a marked reduction in the frequency of the immunoregulatory CD56^brightCD16⁻ endometrial Natural Killer (eNK) cells compared to women using no contraceptives (30.1% ± 5.99 and 66.0% ± 10.6; p = 0.001). Additionally, women using copper IUD showed elevated frequencies of proliferating Ki-67⁺CD8⁺ T cells compared to COC users and controls (19.8% ± 4.3, 2.69% ± 1.23, and 3.77% ± 4.4).

Conclusions

This pilot study highlights the importance of considering contraceptive use when studying immune-related reproductive problems.

月经流出物（ME）被广泛用于研究免疫相关生殖疾病。然而，由于对避孕药具对子宫内膜免疫细胞组成的影响了解有限，使用避孕药具的妇女往往被排除在此类研究之外。这一知识差距限制了我们将使用避孕药具的妇女纳入科学调查并解释与避孕药具使用相关的潜在免疫变异的能力。因此，我们的目的是研究避孕是否与子宫免疫系统的差异有关。研究方法：从使用联合口服避孕药（COC, n = 5）、铜质宫内节育器（IUD, n = 3）或未使用避孕药（n = 5）的妇女中收集ME。从ME中分离免疫细胞，用流式细胞术进行分析。使用非配对t检验分析数据，然后使用错误发现率（FDR）方法进行多重检验校正。结果服用避孕药的妇女与未服用避孕药的妇女相比，免疫调节性CD56brightCD16−子宫内膜自然杀伤细胞（eNK）的频率显著降低（30.1%±5.99和66.0%±10.6;p = 0.001）。此外，与COC使用者和对照组相比，使用铜宫内节育器的女性Ki-67+CD8+ T细胞增殖频率升高（19.8%±4.3,2.69%±1.23和3.77%±4.4）。这项初步研究强调了在研究免疫相关生殖问题时考虑使用避孕药具的重要性。

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引用次数: 0

Mitochondrial DNA Copy Number and Risk of Gestational Metabolic Disorders: A Two-Sample Mendelian Randomization Study 线粒体DNA拷贝数和妊娠代谢障碍的风险：一项双样本孟德尔随机研究

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-08-20 DOI: 10.1111/aji.70144

Feng Zhan, Huijuan Yang, Xuemei Li, Yina Guo, Jianbo Wu, Lidan He

Background

Mitochondrial DNA copy number (mtDNA-CN) has been implicated in gestational metabolic disorders (GMD), yet their causal relationships remain unclear. This study employed genetic approaches to investigate potential causal associations between mtDNA-CN and various GMDs.

Methods

We conducted a two-sample Mendelian randomization (MR) analysis utilizing genome-wide association study (GWAS) summary statistics from large-scale populations: mtDNA-CN (n = 395 718), preeclampsia (PE) (n = 267 242), gestational diabetes mellitus (GDM) (n = 123 579), gestational hypertension (GH) (n = 118 990), hyperlipidemia (n = 9714), and obesity (n = 463 010). Independent single-nucleotide polymorphisms (SNPs) were rigorously selected as instrumental variables (IVs) following stringent criteria. The primary analysis employed the fixed-effects inverse variance weighted (IVW) method. Multiple sensitivity analyses, including leave-one-out analysis, Cochran's Q test, and MR-Egger regression, were conducted to assess the robustness of our findings.

Results

The IVW analysis revealed a significant protective association between increased mtDNA-CN and PE risk (OR = 0.6262, 95% CI: 0.4201–0.9335, p = 0.0283). However, no significant associations were observed between mtDNA-CN and other GMDs (all p > 0.05). Sensitivity analyses, including MR-Egger regression, showed no evidence of horizontal pleiotropy (all p > 0.05), supporting the robustness of our findings.

Conclusion

This genetic investigation provides compelling evidence for a potentially protective effect of higher mtDNA-CN against PE development. These findings not only suggest mtDNA-CN as a promising biomarker for PE risk assessment but also offer novel insights into the biological mechanisms underlying PE, potentially informing future preventive strategies and therapeutic interventions.

线粒体DNA拷贝数（mtDNA-CN）与妊娠代谢障碍（GMD）有关，但其因果关系尚不清楚。本研究采用遗传方法调查mtDNA-CN与各种GMDs之间的潜在因果关系。方法利用全基因组关联研究（GWAS）的汇总统计数据，对大型人群进行两样本孟德尔随机化（MR）分析：mtDNA-CN （n = 395 718）、先兆子痫（n = 267 242）、妊娠糖尿病（n = 123 579）、妊娠高血压（n = 118 990）、高脂血症（n = 9714）和肥胖（n = 463010）。独立的单核苷酸多态性（snp）按照严格的标准被严格选择为工具变量（IVs）。初步分析采用固定效应反方差加权（IVW）方法。我们进行了多重敏感性分析，包括留一分析、科克伦Q检验和egger回归，以评估我们研究结果的稳健性。结果IVW分析显示mtDNA-CN增加与PE风险之间存在显著的保护性关联（OR = 0.6262, 95% CI: 0.4201-0.9335, p = 0.0283）。然而，mtDNA-CN与其他GMDs之间没有显著相关性（均p >； 0.05）。敏感性分析，包括MR-Egger回归，没有显示水平多效性的证据（均p >； 0.05），支持我们研究结果的稳健性。结论本遗传学研究为高mtDNA-CN对PE发育的潜在保护作用提供了强有力的证据。这些发现不仅表明mtDNA-CN是一种有前景的PE风险评估生物标志物，而且为PE的生物学机制提供了新的见解，可能为未来的预防策略和治疗干预提供信息。

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引用次数: 0

Leuko-Glycemic Index and Liver Fibrosis Markers in Gestational Diabetes Prediction: A Novel Perspective 白细胞升糖指数和肝纤维化标志物在妊娠糖尿病预测中的应用：一个新的视角

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-08-20 DOI: 10.1111/aji.70136

Gizem Aktemur, Nazan Vanlı Tonyalı, Betül Tokgöz Çakır, Gülsan Karabay, Zeynep Seyhanli, Ahmet Arif Filiz, Mevlüt Bucak, Serap Topkara Sucu, İslam Aslanlı, Mehmet Ünsal, Ali Turhan Çağlar

Objective

This study investigates the potential role of the Leuko-Glycemic Index (LGI) and liver fibrosis markers, specifically the Fibrosis-4 Index (FIB-4) and Aspartate Aminotransferase/Platelet Ratio Index (APRI), in predicting gestational diabetes mellitus (GDM) and its association with adverse neonatal outcomes.

Methods

A retrospective cohort study was conducted at Ankara Etlik City Hospital, including 1173 pregnant women, of whom 576 (49.1%) were diagnosed with GDM and 597 (50.9%) served as controls. LGI, APRI, and FIB-4 scores were calculated using first- and second-trimester laboratory data. Statistical analyses, including logistic regression and receiver operating characteristic (ROC) curve analysis, were performed to assess their predictive value for GDM and neonatal outcomes.

Results

LGI was significantly lower in the GDM group during both the first and second trimesters (p < 0.001). ROC analysis demonstrated that LGI had a statistically modest discriminative ability for GDM (AUC = 0.583 and 0.609 for the first and second trimesters, respectively, p < 0.001). However, FIB-4 and APRI indices were not significant predictors of GDM. First-trimester APRI scores were significantly associated with adverse neonatal outcomes (AUC = 0.607, p = 0.004).

Conclusion

LGI was identified as a potential predictive marker for GDM, supporting its role as an inflammation-related index in GDM risk assessment. While APRI scores in the first trimester correlated with adverse neonatal outcomes, FIB-4, and APRI indices were not effective predictors of GDM. Further prospective studies with larger cohorts are needed to confirm these findings and explore the clinical applicability of LGI in early GDM screening.

目的探讨白细胞-血糖指数（LGI）和肝纤维化标志物，特别是纤维化-4指数（FIB-4）和天冬氨酸转氨酶/血小板比率指数（APRI）在预测妊娠糖尿病（GDM）及其与新生儿不良结局的关系中的潜在作用。方法在安卡拉Etlik市医院进行回顾性队列研究，纳入1173例孕妇，其中576例（49.1%）诊断为GDM， 597例（50.9%）为对照组。LGI、APRI和FIB-4评分采用妊娠早期和中期实验室数据计算。统计分析，包括逻辑回归和受试者工作特征（ROC）曲线分析，评估其对GDM和新生儿结局的预测价值。结果GDM组妊娠早期和中期LGI均显著降低（p < 0.001）。ROC分析显示LGI对GDM的判别能力在统计学上不高（妊娠早期和中期的AUC分别为0.583和0.609,p < 0.001）。然而FIB-4和APRI指数并不是GDM的显著预测因子。妊娠早期APRI评分与新生儿不良结局显著相关（AUC = 0.607, p = 0.004）。结论LGI可作为GDM的潜在预测指标，支持其在GDM风险评估中的炎症相关指标作用。虽然妊娠早期APRI评分与新生儿不良结局相关，但FIB-4和APRI指数并不是GDM的有效预测指标。进一步的前瞻性研究需要更大的队列来证实这些发现，并探讨LGI在早期GDM筛查中的临床适用性。

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引用次数: 0

Association Between SARS-COV-2 Infection and Sperm DNA Fragmentation: A Systematic Review and Meta-Analysis SARS-COV-2感染与精子DNA断裂的关系：系统综述和荟萃分析

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology

Pub Date : 2025-08-19 DOI: 10.1111/aji.70143

Zahra Asadi, Asad Vaisi-Raygani, Roya Safari-Faramani, Mahmoud Ghasemi, Faranak Aghaz

Introduction

SARS-CoV-2 infection affects various sperm quality parameters. This study examines the impact of COVID-19 infection on sperm DNA fragmentation (SDF).

Methods

A systematic literature search was performed across four databases for studies published between January 1, 2019, and January 1, 2025. The inclusion criteria focused on studies evaluating sperm DNA fragmentation in healthy men infected with the virus. The risk of bias was assessed using the Newcastle–Ottawa scale (NOS). A meta-analysis was conducted using a random effects model based on the tests employed in the studies to measure SDF. Data were reported as weighted mean differences (WMD) and corresponding 95% confidence intervals (CI). Out of 105 identified citations, seven articles were included in this analysis. The NOS results indicated that all studies were of high quality. Subgroup analysis revealed that all testing methods, including TUNEL, flow cytometry, and the sperm chromatin dispersion (SCD) test, demonstrated high heterogeneity, with the lowest heterogeneity found in the TUNEL test.

Results

The pooled analysis indicated a statistically significant increase in SDF (random effects model, WMD = 12.558, 95% CI: 4.482 to 20.635, I² = 99%, Z = 3.05, p < 0.0001). This meta-analysis suggests a statistically significant reduction in sperm DNA integrity 2–3 months following COVID-19 infection.

Conclusion

However, caution is warranted when interpreting these results due to the high heterogeneity, which may affect the outcomes. A thorough analysis considering participant characteristics and infection status is recommended.

SARS-CoV-2感染影响精子各项质量参数。本研究探讨了COVID-19感染对精子DNA片段化（SDF）的影响。方法系统检索2019年1月1日至2025年1月1日期间发表的4个数据库的文献。纳入标准侧重于评估感染该病毒的健康男性精子DNA断裂的研究。偏倚风险采用纽卡斯尔-渥太华量表（NOS）进行评估。采用随机效应模型对研究中采用的SDF测量方法进行meta分析。数据以加权平均差（WMD）和相应的95%置信区间（CI）报告。在105个确定的引用中，有7篇文章被纳入了本分析。NOS结果显示所有研究均为高质量。亚组分析显示，包括TUNEL、流式细胞术和精子染色质分散（SCD）检测在内的所有检测方法均表现出高度的异质性，TUNEL检测的异质性最低。结果合并分析显示，SDF增加有统计学意义（随机效应模型，WMD = 12.558, 95% CI: 4.482 ~ 20.635, I2 = 99%, Z = 3.05, p < 0.0001）。这项荟萃分析表明，在感染COVID-19后2-3个月，精子DNA完整性显著降低。然而，由于高度异质性，在解释这些结果时需要谨慎，这可能会影响结果。建议考虑参与者的特征和感染状况进行彻底的分析。

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引用次数: 0