Zeitschrift fur experimentelle Chirurgie最新文献

The kinetics of proliferation of the ischemically injured small intestine in rats were tested after recovery of the circulation in dependence on the age by means of the autoradiography. The marking index and the middle density of silver grain were applied. The time of the anoxia amounted to 3 hours, since in that case a regeneration can hardly be expected after this time. With a complete regeneration both the number of the synthesizing cells and the synthesized DNA quantity can be calculated after the recovery of circulation. Differences dependent on the age become clear, in a similar way to the physiological kinetics of proliferation. The values of comparison with the control group are reached in rats at an age of 6 weeks already after nearly 18 to 20 hours. On the other hand the time of the regeneration amounted to nearly 36 to 48 hours in the rats at the age of 12 months. The demand of an early diagnosis and recovery of the circulation within 3 hours is emphasized.

The ultrasonic power with regard to its medical use for resection, treatment, and joining of soft and hard biologic tissues is investigated after the formation of the ultrasonic diagnostics and therapy. The situation of the ultrasonic surgery as an unconventional method for the ingenious complementation of tested operative techniques is valued after the representation of the mode of operation and the present equipment. The trend of development and prospective problems of the research are deduced from them.

Investigations on the load capacity of an ultrasonic weld in transverse fractures, oblique fractures, and multiple fragment fractures by means of homologous bone fragments and Fimomed were taken up in vitro on 84 human femur diaphyses. It was possible to demonstrate the essential influence of the form of the fracture on the stability of the weld. The mechanical density was the highest in transverse fractures, small in oblique fractures, and the smallest in multiple fragment fractures. Conclusions were drawn up for the clinical use.

The observations and the results after allogenic lung transplantation in 24 receivers were reported. The animals were not treated immuno-suppressively. They survived 6.2 days on an average and died in consequence of the rejection. The transplant is progressively threatened of bacterial infections after the beginning of the rejection even in case of an operation under sterile conditions. The investigations confirmed a narrow correlation between the leucocyte count and the course of the rejection. However, the condition of the transplant can be estimated approximately certainly by means of complex clinical methods of examination.

The production of an arterial stenosis in an experiment is performed mainly by means of starvation ligatures or constrictors. In this way the produced stenosis does not allow to exercise reconstructive surgical interventions during the training in the angio-surgery. We produced the model of an isolated arterial stenosis by means of an anodic direct current in extravascular application. The stenosis was demonstrated by angiography and measurement of the blood flow. Measurements of the oxygen tension in the femoral artery and vein showed a decrease of the values after producing the stenosis. This method makes it possible to carry out a training of any kind of reconstructive interventions.

1. A selective late dye concentration dependent on the time is described for 3 triphenylmethane dyes namely bromphenol blue, bromcresol green and iodophenol blue after intravenous application in high dosage in malignant inoculated tumors and experimental tumor metastases in the mouse. 2. The possible mechanisms of this dye concentration phenomenon in the tumor tissue as well as some chances of the eventual therapeutic and tumor diagnostic utilization were discussed.

In 14 dogs pancreatectomy was performed and pancreas was submitted to 24 hour hypothermic pulsatile perfusion using Gambro machine. During the perfusion exocrine secretion subsequent to secretin and CCK stimulation was collected and the perfusion fluid was analyzed. In 7 of these animals, Venalot, a combination of coumarin and rutin sulphate, was added to the perfusion fluid and its effect was tested. The addition of Venalot was followed by a decreased vascular peripheral resistance, decreased weight of the perfused graft, and decreased level of LDH and lactic acid in the perfusion fluid. The release of potassium from the pancreatic cells was prevented. The parameters of the pancreatic secretion was better than in the control group. Benzo-pyrones reduced oedema formation and improved cell metabolism during hypothermic perfusion of the pancreas.

Our results, which confirmed on the basis of our experiment that Gentamycin in the bile is extracted by the liver only after prolonged systemic infusion and only in low concentration, contrasted with intraportal infusion of the drug resulting in doses above MIC level after 30 minutes and the difference is highly significant after 60 minutes.

An acute coma hepaticum was produced in 8 pigs by means of one intraperitoneal dose of thioacetamide. Distinct changes in the behaviour of the animals, changes of the biochemical parameters inclusively the enzyme pattern with a typical lesion pattern of the liver parenchyma and a displacement of the plasma amino acids were shown. All pathological changes in comparison with the normal animal characterize the liver insufficiency after 24 hours as a comatose state. This acute toxic model serves as the base for investigations within the physiology of nutrition about parenteral feeding of L-amino acid mixtures in coma hepaticum. The nearly exclusive hepatotropic lesion degree of thioacetamide is suitable as an experimental model for this purpose.