Zeitschrift fur experimentelle Chirurgie最新文献

Rats were differently treated after transplantation of cells of Jensen sarcoma to test the influence of Doparkin (increase of the cAMP level), morbilli vaccine (interferon inductor) and local radio-therapy alone or in combination for a decrease of the rate of growing tumors. The lowest rate of growing tumors was received by combining the agents with a local radio-therapy. The analysis of the thymic factor level showed in the animals with a tumor a significant reduction in comparison to normal animals respectively to animals without tumor formation in consequence of the therapy. An application of the thymic factor alone respectively in combination with Doparkin and morbilli vaccine leads to a clear decrease of the rate of growing tumors.

Contrary observations exist in the publications concerning the bile peritonitis. Therefore the effect of sterile bile in the peritoneal cavity was investigated in the white mouse. We had the following results: 1. The L.D. 50 will be obtained only by high bile concentrations. 2. The chemical bile effect is minimal on the visceral and parietal peritoneum in the early and late phase. 3. The bile peritonitis does not get infected in case of the intact gastrointestinal and biliary tract. There does not exist a so-called passing through peritonitis even with big bile quantities. 4. A reduction of the peritoneal adhesions arises by bile influence and by weak adhesion stimuli.

There was produced a swelling by injection of a defined water quantity into the fascial layers of corpse shanks. Then dates were found out in connection with the increase of circumference, volume, plane, pressure and wound dehiscence in the swelling state. Information was given on the order of the traction necessary for the knotting of the sutures and also on the operative technique needed therefore.

Authors examined the changes of antibiotic concentration in the inflammatory focus after administering Gentamycin and Cephalothin intravenously or intraarterially in case of experimentally induced osteomyelitis in the tibia of rabbit. Administering Gentamycin i. v. no therapeutic level could be measured, while Cephalothin showed a therapeutic level of antibiotic concentration just for a short period. When the same doses of these antibiotics were administered in the femoral artery, effective local antibiotic concentration could be detected for 3 hours. Authors' clinical studies also support these experimental results. Performing 43 i. a. treatments in 38 patients with osteomyelitis 39 was rated as effective.

An acute myocardial infarction was produced in experiments in dogs by ligature of the ramus anterior of the left coronary artery. Cell damages in the liver showed electron microscopically 90 and 120 minutes after the begin of the experiment. Destruction or disturbance in the permeability of the liver cells was not or slight developed by pretreatment with radiologically detoxicated endotoxin. In this case no aggravation of the liver function was evident parallel to the morphological changes and the ability of the liver to detoxication of endotoxin was reserved. The experiment shows that the protective effect of Tolerin which prevented the ischemic liver cell damage is not the endotoxin tolerance. The stabilizing effect to the lysosomal membrane by Tolerin is the cause likely.

A report is given about comparison measurements of the intracerebral temperature and the temperature of the interior of body in form of the blood temperature in the arteria pulmonalis in dogs with cerebral circulation stop. The decrease of the brain temperature of 0.03 K per minute takes place long before the decrease of the blood temperature. It coincides clearly with other signs of the deficiency of all brain functions. The decrease of the brain temperature can be considered as one of the early symptoms of the cerebral circulation stop.

This paper reports about experimental xenografting of kidneys in closely related species (fox-dog) modifying primary graft rejection by treatment of the recipients with anti-donor brain globulin (ADBG). ADBG, produced by immunisation of rabbits with fox brain tissue, in vitro demonstrates cytotoxicity against fox lymphocytes as well as versus dog lymphocytes. In respect of the phylogenetic constellations, the erythrocytes of both species, too, are influenced in hemagglutinating test. However, kidney tissue is said to be unable to adsorb thymocytolytic antibodies of anti brain sera, thus the way of immunosuppression is directed mostly against the lymphocytes of the recipients, causing here twofold survival time compared to untreated controls. MST is 11.2 +/- 2.4 days (n = 5). Cytotoxic antibodies appear on the 8th day after surgery, that means 4 days later than in the controls. Histological findings correspond to those of untreated recipients.

The measurement of the resistance between the electrodes for stimulation during the operation shows the electrical field strength in the osteotomie. This one will recommend for the comparison of the results of stimulation experiments. The measurement in the course of healing shows faults at the electrodes and the contacts, whereas as the assertions about the process of healing are slightly.

In eight piglets small intestinal blood flow (IBF) and motility has been measured after 48 hrs mechanical obstruction, prostigmin application and manual decompression using the microspheres method. Intraenteric basic pressure is in a normal range; the number of tonic contraction waves (type 3 waves) is slightly increased. IBF after 48 hrs ileus is markedly increased compared to normal (218%). Prostigmin does not influence IBF significantly. After manual decompression, IBF decreases to 56% of normal and does not recover within 30 minutes. Whereas IBF in the segment proximal to the obstruction is increased, IBF in the distal part is significantly reduced (48%) after 48 hrs of ileus. It is concluded that 48 hrs of ileus have no harmful effect on intestinal blood flow and function. Prostigmin does not increase IBF, whereas motility is stimulated strongly. Mechanical decompression results in a 50% reduction of IBF, which might be in accordance with clinical observation of retarded recovery and reduced motility after this maneuvre.