In order to investigate and find out the bacterial community of hot spring waters in Eskisehir, Turkey, 7 hot spring water samples were collected from 7 different hot springs. All samples were inoculated using four different media (nutrient agar, water yeast extract agar, trypticase soy agar, starch casein agar). After incubation at 50 oC for 14 days, all bacterial colonies were counted and purified. Gram reaction, catalase and oxidase properties of all isolates were determined and investigated by BIOLOG, VITEK and automated ribotyping system (RiboPrinter). The resistance of these bacteria was examined against ampiciline, gentamisine, trimethoprime-sulphamethoxazole and tetracycline. As a result, heat resistant pathogenic microorganisms in addition to human normal flora were determined in hot spring waters (43-50 oC) in investigated area. Ten different species belong to 6 genera were identified as Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans and Pseudomonas taetrolens.
{"title":"MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY","authors":"N. Y. Sarıözlü, Rasime Demirel, M. Kıvanç","doi":"10.18036/BTDC.91655","DOIUrl":"https://doi.org/10.18036/BTDC.91655","url":null,"abstract":"In order to investigate and find out the bacterial community of hot spring waters in Eskisehir, Turkey, 7 hot spring water samples were collected from 7 different hot springs. All samples were inoculated using four different media (nutrient agar, water yeast extract agar, trypticase soy agar, starch casein agar). After incubation at 50 oC for 14 days, all bacterial colonies were counted and purified. Gram reaction, catalase and oxidase properties of all isolates were determined and investigated by BIOLOG, VITEK and automated ribotyping system (RiboPrinter). The resistance of these bacteria was examined against ampiciline, gentamisine, trimethoprime-sulphamethoxazole and tetracycline. As a result, heat resistant pathogenic microorganisms in addition to human normal flora were determined in hot spring waters (43-50 oC) in investigated area. Ten different species belong to 6 genera were identified as Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans and Pseudomonas taetrolens.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"2 1","pages":"31-39"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu calismada; Civril Golu’nun fizikokimyasal parametreleri 2004-2005 yillari arasinda aylik olarak olculmustur. Olcumler, Civril Golu’nu en iyi temsil edecek sekilde secilen dort istasyonda yapilmistir. Elde edilen yillik oratalama fizikokimyasal parametre verileri istasyonlar arasinda istatistiksel olarak karsilastirilmis, faktor analizi ile degiskenler siniflandirilmistir. Butun istasyonlarda yil boyu gol suyunun berrak ve kokusuz, tuzlulugun %0.0, hidroksilin ise 0 mg/L oldugu tespit edilmistir. Faktor analizi sonucuna gore tum istasyonlarda veri kumesinde toplam varyansin % 100’unu aciklayan uc potansiyel faktor belirlenmistir. Ilk faktor toplam varyansin % 58.98’ini, ikinci faktor % 21.51’ini ucuncu faktor ise % 19.50’sini aciklamistir. Bu calisma; Civril Golu’nun bazi istasyonlarinda ozellikle yaz aylarinda organik kirliligin oldukca arttigini ve cozunmus oksijen miktarinin da ozellikle 1. istasyonda sucul canlilar icin kritik dereceye kadar azaldigini gostermistir.
在灾难中，Civril Golu的物理参数是在2004年至2005年间分别观测到的。它是在筷子站建造的，当时Civril Golu最有代表性。Elde edilen yillik or talama fizikokimysal参数是一个真实的参数，它是一个完整的参数。但是，如果你不服用kokusuz，tuzlulugun%0.0，hidroksilin则为0mg/L。因子分析的结果是，如果数据框中总共有100%，则定义了较低的潜在因子。如果第一个因素发生在58.98%，而第二个因素出现在21.51%，则减少19.50%。Bu calisma；在Civril Golu的一些站点，有机污染在夏季月份有所增加，cozunmus的氧气含量在氧化物1中有所增加。在空间站，水动物被减少到临界水平是最重要的。
{"title":"EVALUATION OF SURFACE WATER QUALITY IN ÇIVRIL LAKE","authors":"C. Bulut, Ramazan Atay, K. Uysal, E. Köse","doi":"10.18036/BTDC.58853","DOIUrl":"https://doi.org/10.18036/BTDC.58853","url":null,"abstract":"Bu calismada; Civril Golu’nun fizikokimyasal parametreleri 2004-2005 yillari arasinda aylik olarak olculmustur. Olcumler, Civril Golu’nu en iyi temsil edecek sekilde secilen dort istasyonda yapilmistir. Elde edilen yillik oratalama fizikokimyasal parametre verileri istasyonlar arasinda istatistiksel olarak karsilastirilmis, faktor analizi ile degiskenler siniflandirilmistir. Butun istasyonlarda yil boyu gol suyunun berrak ve kokusuz, tuzlulugun %0.0, hidroksilin ise 0 mg/L oldugu tespit edilmistir. Faktor analizi sonucuna gore tum istasyonlarda veri kumesinde toplam varyansin % 100’unu aciklayan uc potansiyel faktor belirlenmistir. Ilk faktor toplam varyansin % 58.98’ini, ikinci faktor % 21.51’ini ucuncu faktor ise % 19.50’sini aciklamistir. Bu calisma; Civril Golu’nun bazi istasyonlarinda ozellikle yaz aylarinda organik kirliligin oldukca arttigini ve cozunmus oksijen miktarinin da ozellikle 1. istasyonda sucul canlilar icin kritik dereceye kadar azaldigini gostermistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"2 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was conducted to investigate the effect of UV-B radiation with different exposure times (0, 1, 3 and 5 hours) on photosystem II (PSII) efficiency of barley (Hordeum vulgare L., Bulbul 89) leaves. The polyphasic rise (OJIP) in the chlorophyll a fluorescence was used to evaluate the effect of UV-B stress on the efficiency of PSII. UV-B stress significantly affected the functional parameters and structural parameters calculated by JIP-Test. The effect of UV-B with different application times on polyphasic chlorophyll a fluorescence parameters are discussed from the perspective of evaluating the relative importance of UV-B of donor and acceptor side capacity in PSII.
{"title":"THE EFFECT OF ULTRAVIOLET-B STRESS ON PHOTOSYSTEM II EFFICIENCY IN BARLEY PLANT","authors":"N. Çiçek, H. Çakırlar, R. Strasser","doi":"10.18036/BTDC.48233","DOIUrl":"https://doi.org/10.18036/BTDC.48233","url":null,"abstract":"This study was conducted to investigate the effect of UV-B radiation with different exposure times (0, 1, 3 and 5 hours) on photosystem II (PSII) efficiency of barley (Hordeum vulgare L., Bulbul 89) leaves. The polyphasic rise (OJIP) in the chlorophyll a fluorescence was used to evaluate the effect of UV-B stress on the efficiency of PSII. UV-B stress significantly affected the functional parameters and structural parameters calculated by JIP-Test. The effect of UV-B with different application times on polyphasic chlorophyll a fluorescence parameters are discussed from the perspective of evaluating the relative importance of UV-B of donor and acceptor side capacity in PSII.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"2 1","pages":"9-19"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The microbiological quality of bottled natural spring and mineral water, purchased at different markets in Eskisehir (Turkey) markets, was investigated. Applying the membrane filtration method, the aliquots of water samples were analyzed for the presence and enumeration of total coliforms, Escherichia coli, Enterococcus spp., Aeromonas hydrophila . Aerobic bacteria were counted as Heterotrophic Bacteria Count (HPC) ml-1 by incubation at 22 and 37 oC. While Bacillus species in bottled mineral water samples have also been determined, any bacteria or contamination in bottled drinking water samples have not found.
{"title":"MICROBIOLOGICAL INVESTIGATION OF BOTTLED MINERAL AND DRINKING WATERS SOLD IN ESKİŞEHİR (TURKEY) MARKETS","authors":"Rasime Demirel, N. Y. Sarıözlü, M. Kıvanç","doi":"10.18036/BTDC.26550","DOIUrl":"https://doi.org/10.18036/BTDC.26550","url":null,"abstract":"The microbiological quality of bottled natural spring and mineral water, purchased at different markets in Eskisehir (Turkey) markets, was investigated. Applying the membrane filtration method, the aliquots of water samples were analyzed for the presence and enumeration of total coliforms, Escherichia coli, Enterococcus spp., Aeromonas hydrophila . Aerobic bacteria were counted as Heterotrophic Bacteria Count (HPC) ml-1 by incubation at 22 and 37 oC. While Bacillus species in bottled mineral water samples have also been determined, any bacteria or contamination in bottled drinking water samples have not found.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"153-160"},"PeriodicalIF":0.0,"publicationDate":"2011-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Özdemir, Z. Kaplancıklı, G. Turan-Zitouni, M. Altıntop, Fatih Demirci
The aim of this study is to synthesize novel benzothiazolyl-amide derivatives and investigate their antimicrobial activities. N-(Benzothiazol-2-yl)-2-(4H-[1,2,4]triazol-3-ylsulfanyl) acetamide derivatives were prepared by the reaction of N-(benzothiazol-2-yl)-2-chloroacetamides with appropriate 4H-[1,2,4]triazol-2-thione. The chemical structures of the compounds were elucidated by elemental analyses, IR, 1 H-NMR, 13 C-NMR and FAB + -MS spectral data. Their antimicrobial activities against Escherichia coli (NRRL B-3008), Staphylococcus aureus ( ATCC 6538) , Pseudomonas aeruginosa ( ATCC 27853), Proteus vulgaris (NRRL B-123), Salmonella typhimurium (ATCC 13311), Methicillin-resistant Staphylococcus aureus (MRSA) ( clinic isolate), Candida albicans (NRRL Y-12983) and Candida parapsilosis (NRRL Y-12696) were investigated. The results showed that all of the tested compounds were inactive against the test organism.
{"title":"SYNTHESIS OF SOME N-(BENZOTHIAZOL-2-YL)-2-(4H-(1,2,4)TRIAZOL -3- YLSULFANYL)ACETAMIDES AND THEIR ANTIMICROBIAL ACTIVITY","authors":"A. Özdemir, Z. Kaplancıklı, G. Turan-Zitouni, M. Altıntop, Fatih Demirci","doi":"10.18036/BTDC.71621","DOIUrl":"https://doi.org/10.18036/BTDC.71621","url":null,"abstract":"The aim of this study is to synthesize novel benzothiazolyl-amide derivatives and investigate their antimicrobial activities. N-(Benzothiazol-2-yl)-2-(4H-[1,2,4]triazol-3-ylsulfanyl) acetamide derivatives were prepared by the reaction of N-(benzothiazol-2-yl)-2-chloroacetamides with appropriate 4H-[1,2,4]triazol-2-thione. The chemical structures of the compounds were elucidated by elemental analyses, IR, 1 H-NMR, 13 C-NMR and FAB + -MS spectral data. Their antimicrobial activities against Escherichia coli (NRRL B-3008), Staphylococcus aureus ( ATCC 6538) , Pseudomonas aeruginosa ( ATCC 27853), Proteus vulgaris (NRRL B-123), Salmonella typhimurium (ATCC 13311), Methicillin-resistant Staphylococcus aureus (MRSA) ( clinic isolate), Candida albicans (NRRL Y-12983) and Candida parapsilosis (NRRL Y-12696) were investigated. The results showed that all of the tested compounds were inactive against the test organism.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"145-151"},"PeriodicalIF":0.0,"publicationDate":"2011-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Determination of larval growth rate of and forensic analysis of the age of Calliphoridae larvae on a corpse are useful evidence in legal investigations for the estimation of exact death time and time duration after death; post mortem interval. However many factors, such as temperature, tissue type and contamination of drugs and toxins, effect larval development of blow fly larvae and consequently the estimation of post mortem interval. The present study examined the larval growth rate of a forensically important blow fly species, Lucilia sericata Meigen 1826 in different concentrations (0,12; 0,25; 0,50; 1 and 2 μg/g) of toxic heavy metal Thallium under controlled laboratory conditions. Body length and weight, death ratio of larvae and pupa between experimental and control groups were compared. Results demonstrated that the development rate of larvae between uncontaminated and contaminated diets varies significantly. In short, they molted later, reached maximum length more slowly and sometimes produced significantly smaller pupae in contaminated food source. These results emphasized that the importance of determining the contamination rate of toxins in tissue for the forensic entomologist, while using development rates from standard curves based on larvae fed non-contaminated mediums.
{"title":"EFFECTS OF THALLIUM ON THE LARVAL DEVELOPMENT OF LUCILIA SERICATA MEIGEN 1826 AND PMI ESTIMATION","authors":"F. Altunsoy, Arif Gökhan Başaran","doi":"10.18036/BTDC.78601","DOIUrl":"https://doi.org/10.18036/BTDC.78601","url":null,"abstract":"Determination of larval growth rate of and forensic analysis of the age of Calliphoridae larvae on a corpse are useful evidence in legal investigations for the estimation of exact death time and time duration after death; post mortem interval. However many factors, such as temperature, tissue type and contamination of drugs and toxins, effect larval development of blow fly larvae and consequently the estimation of post mortem interval. The present study examined the larval growth rate of a forensically important blow fly species, Lucilia sericata Meigen 1826 in different concentrations (0,12; 0,25; 0,50; 1 and 2 μg/g) of toxic heavy metal Thallium under controlled laboratory conditions. Body length and weight, death ratio of larvae and pupa between experimental and control groups were compared. Results demonstrated that the development rate of larvae between uncontaminated and contaminated diets varies significantly. In short, they molted later, reached maximum length more slowly and sometimes produced significantly smaller pupae in contaminated food source. These results emphasized that the importance of determining the contamination rate of toxins in tissue for the forensic entomologist, while using development rates from standard curves based on larvae fed non-contaminated mediums.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"103"},"PeriodicalIF":0.0,"publicationDate":"2011-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this century, the amount of industrial produces and their consumption has increased tremendously. Along with this increase, accumulation of industrial waste and its effect on nature has caused serious problems. Because of including various chemicals and especially dye, textile waste water is one of the most hazardous industrial wastes. Color is the most important pollutant in waste water and it should be decolorized. Decolorization is more important than degradation of organic substances from waste water. Even a small amount of dye found changes the color of rivers, lakes and other water resources, and reduces the penetration of light and solubility of gases.White rot fungus are used as a biological system in degradation and decolorizing of textile dyes. In this study, parameters for decolorization of several textile dyes (Blue 49, Orange 12, Orange 13, Red 31, Black 5, RBBR) by newly isolated Trametes versicolor M96was studied. It has been determined that pH, amount of inoculum, shaking speed (rpm), dye concentration and temperature are important factors in decolorization of the studied dyes. The maximum decolorization was found pH 4.5, amount of inoculums 50 ml, shaking speed 200 rpm, dye concentration 50 mg/l and heat 30 °C.
{"title":"DECOLORIZATION OF TEXTILE DYES BY NEWLY ISOLATED TRAMETES VERSICOLOR STRAIN","authors":"S. Pilatin, B. Kunduhoglu","doi":"10.18036/BTDC.83482","DOIUrl":"https://doi.org/10.18036/BTDC.83482","url":null,"abstract":"In this century, the amount of industrial produces and their consumption has increased tremendously. Along with this increase, accumulation of industrial waste and its effect on nature has caused serious problems. Because of including various chemicals and especially dye, textile waste water is one of the most hazardous industrial wastes. Color is the most important pollutant in waste water and it should be decolorized. Decolorization is more important than degradation of organic substances from waste water. Even a small amount of dye found changes the color of rivers, lakes and other water resources, and reduces the penetration of light and solubility of gases.White rot fungus are used as a biological system in degradation and decolorizing of textile dyes. In this study, parameters for decolorization of several textile dyes (Blue 49, Orange 12, Orange 13, Red 31, Black 5, RBBR) by newly isolated Trametes versicolor M96was studied. It has been determined that pH, amount of inoculum, shaking speed (rpm), dye concentration and temperature are important factors in decolorization of the studied dyes. The maximum decolorization was found pH 4.5, amount of inoculums 50 ml, shaking speed 200 rpm, dye concentration 50 mg/l and heat 30 °C.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"125"},"PeriodicalIF":0.0,"publicationDate":"2011-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Detoxification of a chlorinated phenolic compound, 2,4,6-trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymatic dechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuously to detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium. The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6-trichlorophenol by the laccase under the determined optimum conditions.
{"title":"DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR","authors":"Arzu Ünal, A. Çabuk, Nazif Kolonkaya","doi":"10.18036/BTDC.84054","DOIUrl":"https://doi.org/10.18036/BTDC.84054","url":null,"abstract":"Detoxification of a chlorinated phenolic compound, 2,4,6-trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymatic dechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuously to detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium. The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6-trichlorophenol by the laccase under the determined optimum conditions.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"113"},"PeriodicalIF":0.0,"publicationDate":"2011-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Origanum onites L. ucucu yagi ve onun fenolik bileseni olan karvakrolun insan kanserli karaciger hucreleri Hep-G2 uzerindeki sitotoksik ve apoptotik etkileri calisilmistir. Hucrelerin cogalmasi uzerine origanum ucucu yaginin ve karvakrolun engelleyici etkilerini belirlemek icin WST-1 ve notral kirmizisi alim yontemleri uygulanmistir. Origanum yagi ve karvakrolun olasi apoptotik etkisi akridinoranj/etidyumbromur (AO/EB) boyama yontemiyle arastirilmistir. Sonuclar degerlendirildiginde, Origanum yagi ve karvakrolun Hep-G2 hucrelerinde anlamli derecede sitotoksik etkiye sahip oldugu ve bu hucrelerde apoptozu uyardigi gozlenmistir. Ucucu yagin IC50 degeri % 0.009, karvakrolun 500 mM olarak belirlenmistir. Origanum ucucu yagi ve karvakrol ile inkubasyonun ardindan hucrelerin kromatin yogunlasmasi, hucre buzulmesi, sitoplazmik keselerin olusumu gibi karakteristik apoptoz morfolojisi gosterdigi belirlenmistir. Sonuc olarak, hem Origanum ucucu yagi hem de onun temel bileseni olan karvakrol, insan kanserli karaciger hucrelerinde anlamli bir sitotoksik etki ve apoptotik aktivite sergilemistir ve bu ozelliklerinden dolayi, her ikisi de antikanser ajan olarak kullanilabilme potansiyeline sahiptir
{"title":"ANTIPROLIFERATIVE AND APOPTOTIC EFFECTS OF THE ESSENTIAL OIL OF ORIGANUM ONITES AND CARVACROL ON HEP-G2 CELLS","authors":"Hülya Sivas, Özlem Tomsuk","doi":"10.18036/BTDC.44382","DOIUrl":"https://doi.org/10.18036/BTDC.44382","url":null,"abstract":"Origanum onites L. ucucu yagi ve onun fenolik bileseni olan karvakrolun insan kanserli karaciger hucreleri Hep-G2 uzerindeki sitotoksik ve apoptotik etkileri calisilmistir. Hucrelerin cogalmasi uzerine origanum ucucu yaginin ve karvakrolun engelleyici etkilerini belirlemek icin WST-1 ve notral kirmizisi alim yontemleri uygulanmistir. Origanum yagi ve karvakrolun olasi apoptotik etkisi akridinoranj/etidyumbromur (AO/EB) boyama yontemiyle arastirilmistir. Sonuclar degerlendirildiginde, Origanum yagi ve karvakrolun Hep-G2 hucrelerinde anlamli derecede sitotoksik etkiye sahip oldugu ve bu hucrelerde apoptozu uyardigi gozlenmistir. Ucucu yagin IC50 degeri % 0.009, karvakrolun 500 mM olarak belirlenmistir. Origanum ucucu yagi ve karvakrol ile inkubasyonun ardindan hucrelerin kromatin yogunlasmasi, hucre buzulmesi, sitoplazmik keselerin olusumu gibi karakteristik apoptoz morfolojisi gosterdigi belirlenmistir. Sonuc olarak, hem Origanum ucucu yagi hem de onun temel bileseni olan karvakrol, insan kanserli karaciger hucrelerinde anlamli bir sitotoksik etki ve apoptotik aktivite sergilemistir ve bu ozelliklerinden dolayi, her ikisi de antikanser ajan olarak kullanilabilme potansiyeline sahiptir","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"171-180"},"PeriodicalIF":0.0,"publicationDate":"2011-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Yıldız, H. Terzi, Süleyman Cenkci, Suna Arikan Terzi, Behiye Uruşak
Salt stress limits plant productivity in arid and semi arid regions. Salt stress causes decrease in plant growth by adversely affecting physiological processes, especially photosynthesis. Salinity tolerance is defined as the ability of plant to maintain normal growth and development under salt conditions. Salt stress results in accumulation of low molecular weight compounds, termed compatible solutes, which do not interfere with the normal biochemical reactions. These compatible solutes such as carbohydrates, polyols, amino acids and amides, quaternary ammonium compounds, polyamines and soluble proteins may play a crucial role in osmotic adjustment, protection of macromolecules, maintenance of cellular pH and detoxification of free radicals. On the other hand, plants subjected to environmental stresses such as salinity produce reactive oxygen species (ROS) and these ROS are efficiently eliminated by antioxidant enzyme systems. In plant breeding studies, the use of some physiological and biochemical markers for improving the salt tolerance in plants is crucial. In this review, the possibility of using some physiological and biochemical markers as selection criteria for salt tolerance is discussed.
{"title":"PHYSIOLOGICAL AND BIOCHEMICAL MARKERS OF SALINITY TOLERANCE IN PLANTS","authors":"M. Yıldız, H. Terzi, Süleyman Cenkci, Suna Arikan Terzi, Behiye Uruşak","doi":"10.18036/BTDC.70870","DOIUrl":"https://doi.org/10.18036/BTDC.70870","url":null,"abstract":"Salt stress limits plant productivity in arid and semi arid regions. Salt stress causes decrease in plant growth by adversely affecting physiological processes, especially photosynthesis. Salinity tolerance is defined as the ability of plant to maintain normal growth and development under salt conditions. Salt stress results in accumulation of low molecular weight compounds, termed compatible solutes, which do not interfere with the normal biochemical reactions. These compatible solutes such as carbohydrates, polyols, amino acids and amides, quaternary ammonium compounds, polyamines and soluble proteins may play a crucial role in osmotic adjustment, protection of macromolecules, maintenance of cellular pH and detoxification of free radicals. On the other hand, plants subjected to environmental stresses such as salinity produce reactive oxygen species (ROS) and these ROS are efficiently eliminated by antioxidant enzyme systems. In plant breeding studies, the use of some physiological and biochemical markers for improving the salt tolerance in plants is crucial. In this review, the possibility of using some physiological and biochemical markers as selection criteria for salt tolerance is discussed.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":"1-33"},"PeriodicalIF":0.0,"publicationDate":"2011-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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