Food & Function最新文献

Conjugated linoleic acid (CLA) is widely used as a dietary supplement due to its reported benefits in enhancing immunity, regulating inflammation, treating obesity, and preventing cancer. However, there is a lack of comprehensive studies on its mechanisms and dose-effect relationships. Moreover, there are insufficient in-depth studies on CLA's new functions, safety, side effects, and clinical utility. This review systematically examines the structure and sources of CLA, summarizes its role in improving human health, and critically reviews the potential mechanisms behind these benefits. It also analyzes the side effects of CLA and addresses issues related to dosing and oxidative decomposition in CLA research. Additionally, the potential of using CLA-producing probiotics to manage diseases is explored. This review can guide and promote further research on CLA's functions and support the development of CLA dietary supplements. It will accelerate the development of CLA nutritional and medical foods, contribute to the improvement of human health, and have important social meaning and economic value.

Objectives: This study aimed to investigate short-term and long-term impact of avocado consumption without caloric restriction on the gut microbiota of free-living adults with abdominal obesity. Methods: The Habitual Diet and Avocado Trial (HAT) was a 26-week, multi-center, randomized, controlled trial involving 1008 individuals with abdominal obesity. Participants were randomly assigned to the Avocado Supplemented Diet Group (AVO), receiving one avocado per day, or the Habitual Diet group (HAB), maintaining their usual dietary habits. Fecal samples were collected at baseline, week 4 and week 26 from a subset of participants recruited at a University of California Los Angeles site (n = 230). Fecal microbiota was assessed with shotgun metagenomics sequencing. Alpha diversity was assessed using the Chao1 and Shannon indices; beta diversity was assessed using Bray–Curtis dissimilarity with significance determined by repeated measures permutational multivariat analysis of variance. Potential association of intervention at week 4 and 26 with alpha diversity, species and metabolic pathways was examined using linear mixed effect models. Results: Compared to the HAB group, the AVO group had higher alpha diversity by 4 weeks, which persisted through the 26-week study period. Exploratory analysis based on healthy eating index-2015 (HEI-2015) indicated that participants with a low HEI score at baseline (≤52.7), had an increase in alpha diversity in the AVO group vs. HAB group. The AVO group had a significant change in beta diversity at week 26 compared to the HAB group. At the species level, the AVO group had significantly increased Faecalibacterium prausnitzii and Bacterium AF16_15 at week 26 compared to the HAB group. Functional analysis showed no significant difference in metabolic pathways between the HAB and AVO groups. Conclusions: Our findings document a potentially favorable effect of avocados on gut microbiota diversity. The prebiotic potential of avocados is more pronounced in individuals with a low diet quality score. This trial is registered at clinicaltrials.gov as NCT03528031 (https://clinicaltrials.gov/study/NCT03528031).

Chronic fatigue syndrome (CFS) is a long-term chronic condition that predisposes individuals to oxidative stress and disruption of the gut microbiota. In this study, sweet corn cob polysaccharide selenium nanoparticles (U-SCPSeNPs) with relatively small particle sizes were prepared using an ultrasound-assisted method. Transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and ultraviolet-visible spectroscopy (UV-Vis) were used to characterize the U-SCPSeNPs and determine the monosaccharide composition of the U-SCPSeNPs. The U-SCPSeNPs were used to improve the CFS of the mice. The results showed that the ultrasound-assisted method reduced the particle size of the SeNPs, and U-SCPSeNPs with a particle size of 76.74 nm and a selenium content of 186.83 ± 7.80 mg g⁻¹ were obtained at an ultrasonication time of 40 min. Sweet corn cob (SCP) bound to the SeNPs through hydrogen bonding. In terms of energy production, the production capacity of Na⁺–K⁺-ATP, Mg²⁺-ATP, and Ca²⁺-ATP was enhanced by U-SCPSeSCP in CFS mice; In terms of oxidative stress, the levels of SOD and MDA were decreased and CAT and GSH-Px were increased by SCPSeSCP. U-SCPSeSCP improved the diversity and abundance of the gut microbiota in CFS mice, and decreasing the relative abundance of Firmicutes increased the relative abundance of Bacteroidota at the phylum level. This study provides a reference for synthesizing polysaccharide SeNPs and assessing the ability of U-SCPSeNPs to alleviate CFS.

This research aimed to analyze the percentage of short-chain fatty acids (SCFAs) in human milk (HM) and newborn feces and to explore potential associations with factors such as maternal nutrition, age, biological sex, delivery mode, diet, and the type of HM. Gas chromatography was used to measure the percentage of SCFAs in colostrum (n = 23), transitional HM (n = 23), and mature HM (n = 92) and feces of newborn (n = 36) at day 30 postpartum. Anthropometry was also evaluated in the mother and the infant. The results showed that acetic acid was the most abundant in HM. The percentage of butyric acid and isovaleric acid was higher (p < 0.05) in the feces of newborns whose mothers were overweight/obese or were male, respectively, compared to newborns whose mothers were of normal weight or were female. The percentage of valeric acid was higher in the feces of newborns whose mothers were over 30 years old and who were delivered by C-section, compared to newborns whose mothers were 30 years old or younger and who were delivered vaginally. Inadequate intake of proteins, carbohydrates, and fiber was associated (p < 0.05) with lower acetic acid and higher butyric acid, higher propionic acid and lower butyric acid, and higher isovaleric acid percentage, respectively, in mature HM. The percentage of acetic acid was higher (p < 0.01) and that of propionic acid, butyric acid, isobutyric acid, and isovaleric acid was lower (p < 0.01) in colostrum compared to mature HM. The intake of lipids was associated with the percentage of butyric acid (β = −0.32, p = 0.01), and the percentage of propionic acid (β = 0.43, p < 0.01) was associated with carbohydrate intake. Overall, this study concluded that factors such as maternal nutritional status, diet, age, biological sex, and delivery mode were related to the composition of specific SCFAs in mature HM and newborn feces. Additionally, the percentage of SCFAs gradually decreased from colostrum to mature HM.

Broccoli is recognized for its health benefits, attributed to the high concentrations of glucoraphanin (GR). GR must be hydrolyzed by myrosinase (Myr) to form the bioactive sulforaphane (SF). The primary challenge in delivering SF in the upper gastrointestinal (GI) tract- is improving hydrolysis of GR to SF. Here, we optimized the formulation and delivery methods to improve GR conversion and SF bioavailability. We investigated whether the combination of GR-rich broccoli seed extract powder (BSE[GR]) with Myr-rich mustard seed powder (MSP[Myr]), ± ascorbic acid (AA, a co-factor of Myr), delivered as free powder or encapsulated powder, can: (i) facilitate GR hydrolysis to SF during dynamic in vitro gastric digestion and static in vitro small intestinal digestion, and (ii) increase SF bioavailability in Caco-2 cell monolayers, a model of human intestinal epithelium. Addition of exogenous Myr increased the conversion of GR to SF in free powder during small intestinal digestion, but not during gastric digestion, where Myr activity was inhibited by the acidic environment. Capsule delivery of BSE[GR]/MSP[Myr] (w/w ratio 4 : 1) resulted in a 2.5-fold higher conversion efficiency compared to free powder delivery (72.1% compared to 29.3%, respectively). AA combined with MSP[Myr] further enhanced the conversion efficiency in small intestinal digestion and the bioavailability of SF in Caco-2 cell monolayers. Bioavailability of GR as SF, SF metabolites, and GR was 74.8% in Caco-2 cell monolayers following 30 min gastric digestion and 60 min small intestinal digestion. This study highlights strategies to optimize GR bioconversion in the upper GI tract leading to enhanced SF bioavailability.

Citrus polymethoxyflavones (PMFs) have considerable medicinal, health-promoting, and commercial importance. To provide a stable and reliable source of PMFs, an efficient process of large-scale preparation is warranted. Here, an extraction model for enriching PMFs from citrus fruits was proposed using an enzyme/acid-catalyzed hybrid hydrolysis approach. This method was optimized using response surface methodology (RSM). Furthermore, this model was applied to ten citrus varieties to prepare PMF-rich extracts, and six main PMFs were qualitatively and quantitatively analyzed using UPLC-ESI-MS/MS. Among the ten investigated citrus extracts, nobiletin was the most predominant PMF. The total yields of the six PMFs were ranked as C. unshiu > C. reticulata > C. sinensis, indicating that C. unshiu was the most suitable raw material for PMF preparation. Additionally, the PMF-rich extracts showed beneficial regulatory effects on gut microbiota, highlighting their potential health-promoting and therapeutic functions, which warrant further exploration.

Food-derived active peptides (FDAPs) are a class of peptides that exert antioxidant, anti-inflammatory, anti-aging and other effects. In recent years, active peptides from natural foods have been reported to improve skin photoaging, but their mechanisms have not been summarized to date. In this review, we focused on the preparation of FDAPs, their mechanisms of photoaging, and their function against photoaging through the gastrointestinal barrier. Furthermore, the latest progress on FDAPs in the prevention and treatment of skin photoaging via the gut–skin axis is summarized and discussed. FDAPs can be directly absorbed into the gastrointestinal tract and enter skin tissues to exert anti-photoaging effects; they can also regulate the gut microbiota, leading to changes in metabolites to ameliorate light-induced skin aging. Future work needs to focus on the delivery system and clinical validation of anti-photoaging peptides to provide solutions or suggestions for improving photoaging.

Many studies have shown that specific lactic acid bacteria (LAB) strains can delay obesity, offering a viable alternative to medications and surgeries. However, the mining and development of highly effective LAB strains for obesity control is still limited. In this study, the naturally highly acid-tolerant and gamma-aminobutyric acid-producing Levilactobacillus brevis D17 and its glnR deletion strain were used to investigate their anti-obesity effects. In an 8-week mouse experiment, L. brevis D17 and its glnR-deletion strain D17ΔglnR significantly reduced weight gain by 28.4% and 29.1%, respectively, improving abnormal serum indicators and glucose metabolism caused by a high-fat diet. Furthermore, L. brevis D17 and its glnR-deletion strain D17ΔglnR successfully colonized in the gut. Both D17 and D17ΔglnR interventions significantly restored the relative abundance of Muribaculaceae, Ileibacterium valens, Lactobacillus, Faecalibaculum, Bifidobacterium globosum, Akkermansia muciniphila, and Romboutsia ilealis, whereas they significantly reduced potentially harmful bacteria like Leptogranulimonas, Flintibacter, and Alistipes. Additionally, L. brevis intervention effectively decreased the levels of primary bile acids and increased secondary bile acids in the gut, thus balancing bile acid metabolism. The transcriptional analysis suggested that D17 and D17ΔglnR interventions may activate the AMPK signaling pathway in the liver to inhibit lipogenesis, activate the cAMP pathway to promote lipolysis, and inhibit pro-inflammatory macrophage infiltration to block inflammatory responses. These results indicate that L. brevis D17 and its glnR-deletion mutant strain D17ΔglnR show great potential in combating obesity. Moreover, these results also provide insights into the underlying mechanism behind their anti-obesity properties.

Eating rate (ER) can moderate energy intake and ER can be modified by the texture and physical properties of food. However, the magnitude of the effects is not well known. The aim of this study was to investigate how bread texture and physical properties determine ER. In a randomised crossover study, 36 healthy participants (age: 25 ± 6 years, BMI: 22 ± 2 kg m⁻²) consumed nine different bread types. Video coding was used to characterise oral processing behaviour. Sensory texture was evaluated on visual analogue scales. Physical properties were measured using texture profile analysis, puncture tests, geometrical and water-related measures. Two models were developed using response surface methodology (RSM) that predict the ER based on sensory and physical properties. The results showed from slow to fast ER: bread slices < hard buns < soft buns. The slowest bread type (wholemeal bread slice) was consumed 40% slower than the fastest bread type (soft white bun) (P < 0.001), explained by smaller bite sizes and more chews. For the sensory texture, ER was positively correlated with crumb adhesiveness and negatively correlated with crumb dryness. For the physical properties, ER was positively correlated with height and volume, and negatively with crumb cohesiveness and crust hardness. The models based on physical properties (R² = 0.91) and sensory texture (R² = 0.89) were both able to estimate ER, but the model based on physical properties performed slightly better. The insights from the relationships from the sensory and physical measures can both be used to modify the texture of breads, to effectively decrease ER and eventually help to prevent overconsumption.