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Association Between Agronomic and Biomass Component Traits Using Pathway Analysis and Regression Tree Techniques in Biomass Sorghum 利用通路分析和回归树技术分析生物质高粱的农艺性状与生物量成分性状之间的关系
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-07-05 DOI: 10.1007/s12355-024-01437-6
Gabrielle Maria Romeiro Lombardi, José Airton Rodrigues Nunes, Rafael Augusto da Costa Parrella, Michael David Batista Luaemar de Oliveira

The aim of this study was to estimate the correlations among traits in biomass sorghum, estimate the direct and indirect effects, and check for a cause/effect relationship of the traits on the theoretical calorific value (TCV) through pathway analysis. An additional aim is to provide a complementary technique for analysis of the traits and orientation of breeding programs of biomass sorghum through data mining. A total of 25 hybrids of biomass sorghum were evaluated in a triple square lattice design. The following traits were measured: flowering (FLOW); plant height (PH); fresh biomass yield of the stalk (FBYs), of leaves, and of panicles; dry matter of the stalk and of the leaves (DMl); neutral detergent fiber of the stalk and of the leaves; acid detergent fiber of the stalk and of the leaves; lignin of the stalk and of the leaves; hemicellulose of the stalk and of the leaves; cellulose concentration of the stalk and of the leaves; and the TCV. The data were placed under exploratory analysis, the mixed model approach, pathway analysis based on genotypic correlations, and the regression tree technique. The traits FLOW, PH, FBYs, DMl, and LIG were considered the main determinants of the changes in the TCV and, therefore, with potential for use in indirect selection. The technique of data mining allowed better visualization of the relationships among the traits, assisting in some explanations regarding the effect of the traits on the TCV.

本研究旨在估算生物质高粱性状间的相关性,估算直接和间接效应,并通过路径分析检查性状对理论热值(TCV)的因果关系。另一个目的是通过数据挖掘为生物质高粱的性状分析和育种计划定位提供补充技术。在三重方格设计中,共对 25 个生物质高粱杂交种进行了评估。测定了以下性状:开花(FLOW);株高(PH);茎秆、叶片和圆锥花序的新鲜生物量产量(FBYs);茎秆和叶片的干物质(DMl);茎秆和叶片的中性洗涤纤维;茎秆和叶片的酸性洗涤纤维;茎秆和叶片的木质素;茎秆和叶片的半纤维素;茎秆和叶片的纤维素浓度;以及 TCV。对数据进行了探索性分析、混合模型法、基于基因型相关性的路径分析和回归树技术。FLOW、PH、FBYs、DMl 和 LIG 性状被认为是 TCV 变化的主要决定因素,因此有可能用于间接选择。数据挖掘技术使性状之间的关系更加直观,有助于解释性状对 TCV 的影响。
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引用次数: 0
Construction of Gene Expression Profiles of the NAC Transcription Factor Family in Sugar Beet Under Abiotic Stress 构建非生物胁迫下甜菜中 NAC 转录因子家族的基因表达谱
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-07-04 DOI: 10.1007/s12355-024-01436-7
Yaqing Sun, Changlun Li, Zhi Li, Caiyuan Jian, Ningning Li, Shaoying Zhang, Guolong Li

Sugar beet (Beta vulgaris L.) is a major sugar crop in China and an economic crop with regional advantages in the Inner Mongolia Autonomous Region. Currently, abiotic stresses are among the main factors restricting the high-quality development of the sugar beet industry. Recent research has shown that NAC transcription factors play an important role in regulating plant growth and development, as well as in resisting abiotic stress. To determine the specific expression of the NAC transcription factor family in different organs of sugar beet under different abiotic stress conditions, this study systematically analyzed the expression response of 52 members of the NAC transcription factor family in different organs to abiotic stress conditions using reverse transcription polymerase chain reaction technique. The responses of NAC transcription factor genes to different abiotic stresses were identified in sugar beet leaves and roots; 18 and 17 NAC transcription factor genes were found to respond specifically to abiotic stress in sugar beet leaves and roots, respectively. The research results provide a reference basis for using NAC transcription factors to develop stress-resistant germplasm resources in sugar beets.

甜菜(Beta vulgaris L.)是中国的主要糖料作物,也是内蒙古自治区具有区域优势的经济作物。目前,非生物胁迫是制约甜菜产业高质量发展的主要因素之一。最新研究表明,NAC转录因子在调控植物生长发育和抵抗非生物胁迫方面发挥着重要作用。为了确定不同非生物胁迫条件下 NAC 转录因子家族在甜菜不同器官中的特异性表达,本研究利用反转录聚合酶链反应技术系统分析了不同器官中 52 个 NAC 转录因子家族成员对非生物胁迫条件的表达响应。结果发现,甜菜叶片和根系中分别有18个和17个NAC转录因子基因对非生物胁迫有特异性响应。研究结果为利用NAC转录因子开发甜菜抗逆种质资源提供了参考依据。
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引用次数: 0
Coupling Coordination of Green Sugarcane Production and Comprehensive Social Development in Chinese Sugar Industry 中国糖业绿色甘蔗生产与社会综合发展的耦合协调
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-07-04 DOI: 10.1007/s12355-024-01416-x
Yuan Xie, Ting He, Xinyi Chen, Qibin Wu, RuKai Chen, Niandong Chen, Youxiong Que

Coordinated development of green production in sugarcane industry and the economy of the main producing areas is vital for scientifically formulating reduction policies for carbon emission and promoting high-quality industrial development. In the present study, the carbon emissions generated in sugarcane planting process were measured, and the green production efficiency along with comprehensive social development of main sugarcane-producing areas was evaluated and coupled to assess the coordination. The results indicated that carbon emission intensity from sugarcane planting in China decreased from 3.0963 tons/ha in 2012 to 2.4863 tons/ha in 2021, a decrease of 19.70%. The green development efficiency has significantly improved, rising from 0.9228 in 2012 to 1.1478 in 2021. The efficiency in Guangdong and Hainan reached its peak in 2021. Notably, the degree of coupling and coordination between green sugarcane production and comprehensive social development has progressively improved, transitioning from low- to high-level coupling and from serious imbalance to high-quality coordination. In 2021, all regions achieved a high degree of coupling and high-quality coordination. Collectively, reduced application policies in China, accompanied by the development of agricultural technology, have led to a progressive decline in carbon emissions from sugarcane planting while simultaneously improving green production efficiency. Besides, a state of mutual promotion and high-quality coordination was observed in green sugarcane production and local comprehensive social development. This study advances the theoretical framework of carbon emissions and offers empirical evidence for the ecological and economic significance of sugar industry.

甘蔗产业绿色生产与主产区经济协调发展,对于科学制定碳减排政策、推动产业高质量发展至关重要。本研究测定了甘蔗种植过程中产生的碳排放量,并对甘蔗主产区绿色生产效率与社会综合发展的协调性进行了耦合评价。结果表明,中国甘蔗种植碳排放强度从 2012 年的 3.0963 吨/公顷下降到 2021 年的 2.4863 吨/公顷,降幅达 19.70%。绿色发展效率明显提高,从 2012 年的 0.9228 提高到 2021 年的 1.1478。广东和海南的效率在 2021 年达到峰值。值得注意的是,甘蔗绿色生产与社会全面发展的耦合度和协调度逐步提高,由低水平耦合向高水平耦合过渡,由严重失衡向高质量协调过渡。2021 年,所有地区都实现了高度耦合和高质量协调。综合来看,中国的减施政策伴随着农业技术的发展,使甘蔗种植的碳排放量逐步下降,同时提高了绿色生产效率。此外,甘蔗绿色生产与地方社会综合发展呈现出相互促进、优质协调的状态。本研究推进了碳排放的理论框架,为制糖业的生态和经济意义提供了实证依据。
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引用次数: 0
Research Overview on Sugarcane Deep Processing and Comprehensive Utilization in China 中国甘蔗深加工与综合利用研究概况
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-07-03 DOI: 10.1007/s12355-024-01431-y
Gan-Lin Chen, Jing Chen, Bo Lin, Ling-Zhi Zhao, Feng-Jin Zheng, Krishan K. Verma, Yang-Rui Li

The sugar industry is important for the economic development in China. Guangxi province is the largest sugar producer with over 60% of the total sugarcane cultivating area in the country. Research, development, and industrialization on the intensive processing and comprehensive utilization of sugarcane as a raw material will increase in the coming years. This article provides an overview of the development of diversified sugarcane deep processing, focusing on the current research development with special reference to the production of sugarcane wine, vinegar, sugarcane cell water, white sugar, and fructooligosaccharides in China, especially in Guangxi. The aim is to promote the second entrepreneurship of the sugar industry, upgrade the industrial chain, and provide advanced technologies to upgrade the quality and efficiency of the industry and achieve sustainable and healthy socioeconomic development in the near future.

制糖业对中国的经济发展十分重要。广西是全国最大的产糖区,甘蔗种植面积占全国的 60%以上。未来几年,以甘蔗为原料的精深加工和综合利用的研究、开发和产业化程度将不断提高。本文概述了甘蔗深加工多元化的发展情况,重点介绍了中国,特别是广西甘蔗酒、醋、甘蔗细胞水、白砂糖、果寡糖等生产的研究发展现状。目的是促进糖业的二次创业,提升产业链,提供先进技术,提升产业的质量和效益,在不久的将来实现社会经济的可持续健康发展。
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引用次数: 0
Enhancing the Establishment of a Diazotrophic Community in Sugarcane Stem Cutting through Heat–Biocide Treatment 通过热杀菌处理促进甘蔗茎切口重氮营养群落的建立
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-28 DOI: 10.1007/s12355-024-01425-w
Lorraine Cristina Henrique Almeida, Cleudison Gabriel Nascimento da Silva, Gabriela Cavalcanti Alves, Marcia Soares Vidal, Jean Luiz Simões-Araújo, Stefan Schwab, Bruno José Rodrigues Alves, Veronica Massena Reis

The inoculation of diazotrophs in sugarcane presents a challenge due to the plant’s vegetative propagation, which hampers the accurate assessment of the contribution of a new bacterial community in the presence of natural inhabitants. To surmount this obstacle, a modification of short heat treatment (HT—52 °C for 30 min) is normally employed to control ratoon stunt disease, with the addition of a chemical step testing chloride (HCl—1%) and acetic acid (Ac—2%) as biocides. Four experiments were conducted involving two sugarcane cultivars (IACSP95-5000 and RB867515) with and without inoculation of five diazotrophs: G. diazotrophicus, H. seropedicae, H. rubrisubalbicans, N. amazonense, and P. tropica. The HT treatments consisted of control, HT (52 °C for 30 min) alone; HT reduced to 10 min (10HT), biocides: hydrochloric acid (HCl—1%) and acetic acid (Ac—2% at 52 °C for 10 min, and 10HT + 10Ac at room temperature as an additional step—10 AcRT). Plant and bacterial growth (based on two methods—most probable number and qPCR) and nitrogenase activity (acetylene reduction activity—ARA) were measured. Ac was selected as a biocide as germination was not reduced. Inoculation improved germination and sugarcane biomass accumulation, especially with 10 HT + 10 AcRT. The reduction in HT time by 20 min and the addition of Ac (additional step) effectively controlled the natural diazotrophic community, establishing the five diazotrophs, promoting growth, and increasing ARA activity by 82% compared to the traditional HT.

在甘蔗中接种重氮营养体是一项挑战,因为甘蔗是无性繁殖的,这妨碍了在有天然居民存在的情况下准确评估新细菌群落的贡献。为了克服这一障碍,通常采用短时间热处理(HT-52 °C,30 分钟)的方法来控制匍匐茎矮壮病,同时添加一个化学步骤,测试氯化(HCl-1%)和乙酸(Ac-2%)作为杀菌剂。对两个甘蔗品种(IACSP95-5000 和 RB867515)进行了四次实验,分别接种和不接种五种重氮营养体:接种和不接种五种重氮滋养菌:重氮滋养菌 G.、血清型重氮滋养菌 H.、rubrisubalbicans 重氮滋养菌 H.、亚马逊重氮滋养菌 N.和热带重氮滋养菌 P.。高温处理包括:对照组、单独高温处理(52 °C,30 分钟);高温处理缩短至 10 分钟(10HT);杀菌剂:盐酸(HCl-1%)和醋酸(Ac-2%,52 °C,10 分钟;10HT + 10Ac,室温下作为额外步骤-10 AcRT)。对植物和细菌的生长(基于两种方法--最可能数量和 qPCR)和氮酶活性(乙炔还原活性-ARA)进行了测定。由于发芽率没有降低,因此选择 Ac 作为杀菌剂。接种提高了发芽率和甘蔗生物量的积累,尤其是在 10 HT + 10 AcRT 的情况下。与传统 HT 相比,缩短 HT 时间 20 分钟并添加 Ac(额外步骤)可有效控制天然重氮群落,建立五种重氮营养体,促进生长,并将 ARA 活性提高 82%。
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引用次数: 0
Integrated Use of 2,4-D, Metribuzin, and Pyrazosulfuron-Ethyl (UPH 114b) for Managing Weeds and Improving Sugarcane Yield in Subtropical India 在印度亚热带地区综合利用 2,4-D、灭草松和吡唑嘧磺隆(UPH 114b)管理杂草并提高甘蔗产量
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-28 DOI: 10.1007/s12355-024-01441-w
V. P. Jaiswal, S. K. Shukla, Lalan Sharma, V. P. Singh, Asha Gaur, Anand Jha, Abhay Srivastava

A field experiment was conducted with eight treatments of weed management involving integrated and sole applications of 2,4-D, metribuzin, and pyrazosulfuron-ethyl along with weed-free and weedy check practices. The experimental treatments were replicated three times in a randomized block design. Applying integrated product of 2,4-D, metribuzin, and pyrazosulfuron-ethyl effectively managed the grassy (Cynodon dactylon, Eleusine indica, Echinochloa crusgalli, and Digitaria spp.) and broad-leaved weeds (Trianthema monogyna, Chenopodium album, Anagallis arvensis, Melilotus alba, Parthenium hysterophorus, and Bidens frondosa). The higher weed density (124.7 per m2) in weedy check plots was recorded during the first cropping season than 2nd cropping season (118.22/m2). Total weed dry weight was also significantly reduced at the lowest level (43.39 g/m2 during 2019–2020 and 75.70 g/m2 during 2020–2021) with the application of integrated product of 2,4-D, metribuzin, and pyrazosulfuron-ethyl @ 3200 g ai/ha. However, the weed control efficiency (77.57% and 55.59% during 2019–2020 and 2020–2021, respectively) recorded with application of similar herbicide @ 2400 g ai/ha was found at par with its application @3200 g ai/ha. The highest soil organic carbon content (19.90 Mg/ha) was recorded after sugarcane harvest in weedy check plots. The weed management through application of integrated product of 2,4-D, metribuzin, and pyrazosulfuron-ethyl @ 2400 g ai/ha could improve the sugarcane yield by 36.76% (101 tonnes/ha) over the weedy check plots. Increasing dose of the product @3200 g ai/ha could not increase the sugarcane and sugar yields up to significant level. Among all other herbicides treatments (single application) or integrated manner, application of 2,4-D, metribuzin, and pyrazosulfuron-ethyl @2400 g ai/ha also increased sugar yield by 45.12% (11.085 tonnes/ha) over the weedy check plots. The phytotoxicity of the new herbicide product of 2,4-D, metribuzin, and pyrazosulfuron-ethyl could not be recorded in subsequent wheat crops tested up to 4800 g ai/ha.

田间试验采用了八种杂草管理方法,包括综合施用和单独施用 2,4-D、嗪草酮和吡唑嘧磺隆,以及无杂草和杂草检查法。实验处理采用随机区组设计,重复三次。施用 2,4-D、嗪草酮和吡唑嘧磺隆的综合产品可有效控制禾本科杂草(Cynodon dactylon、Eleusine indica、Echinochloa crusgalli 和 Digitaria spp.)和阔叶杂草(Trianthema monogyna、Chenopodium album、Anagallis arvensis、Melilotus alba、Parthenium hysterophorus 和 Bidens frondosa)。与第二季(118.22/m2)相比,第一季杂草检查田的杂草密度更高(124.7/m2)。在施用 2,4-D、嗪草酮和吡唑嘧磺隆(3200 克/公顷)的综合产品后,杂草总干重也明显降低,达到最低水平(2019-2020 年为 43.39 克/平方米,2020-2021 年为 75.70 克/平方米)。然而,施用类似除草剂 @ 2400 g ai/ha 与施用除草剂 @ 3200 g ai/ha 的除草效率(2019-2020 年和 2020-2021 年分别为 77.57% 和 55.59%)相当。甘蔗收获后,杂草对照地块的土壤有机碳含量最高(19.90 兆克/公顷)。通过施用 2,4-D、嗪草酮和吡唑嘧磺隆(施用量为 2400 克/公顷)的综合产品进行杂草管理,甘蔗产量比杂草对照地提高了 36.76%(101 吨/公顷)。增加剂量(3200 克/公顷)并不能显著提高甘蔗和糖的产量。在所有其他除草剂处理(单一施用)或综合方式中,施用 2,4-D、嗪草酮和吡唑嘧磺隆(2400 克/公顷)也比杂草检查地块的糖产量提高了 45.12%(11.085 吨/公顷)。在随后的小麦作物试验中,2,4-D、嗪草酮和吡唑嘧磺隆的植物毒性最高可达 4800 克/公顷。
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引用次数: 0
How Production System Would Affect the Environmental Impacts of Sugar Beet Production? 生产系统如何影响甜菜生产对环境的影响?
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-26 DOI: 10.1007/s12355-024-01413-0
Pejman AliGhaleh, Abbas Rohani, Mohammad Hossein Aghkhani, Armaghan Kosari Moghaddam

Sugar beet production, serving as a key global source of sugar, significantly contributes to the world economy and promotes local economic growth, emphasizing the need for sustainable practices aligned with environmental goals. In this context, it is essential to investigate how production systems may influence the flow of resources and consequently the environmental consequences of sugar beet cultivation in different geographical areas. The present research examined the ecological consequences of sugar beet cultivation in two distinct systems, involving both agro-industries (AIs) and smallholder farmers (SHs), focusing on the Moghan and Joveyn regions, prominent sugar beet production areas in Iran. The study utilized the IMPACT World + life cycle assessment method to evaluate harmful impacts at midpoint and damage levels. Additionally, potential harms were identified within two area of protection (AoPs) categories: human health and ecosystem quality, and two area of concerns (AoCs) categories: carbon-related and water-related damages. The findings revealed that in terms of AoPs, AIs had 0.44% and 4.32% higher impacts on human health than SHs in the Moghan and Joveyn regions, respectively. Additionally, AIs showed 7.97% and 21.44% higher impacts on ecosystem quality compared to SHs in the same regions. Water-related impacts significantly contributed to over 90% of the total environmental impacts in both AoPs. On-farm emissions and nitrogen fertilizers emerged as the primary contributors to the negative effects of sugar beet production in all systems, emphasizing the crucial role of managing chemical fertilizers to mitigate environmental damages, particularly water-related impacts.

甜菜生产是全球糖的主要来源,对世界经济和促进地方经济增长做出了重大贡献,强调了与环境目标相一致的可持续做法的必要性。在这种情况下,有必要研究生产系统如何影响资源流动,进而影响不同地理区域甜菜种植的环境后果。本研究以伊朗著名的甜菜产区 Moghan 和 Joveyn 地区为重点,考察了甜菜种植在两个不同系统中造成的生态后果,这两个系统同时涉及农业企业(AIs)和小农(SHs)。该研究采用了 IMPACT World + 生命周期评估方法,以评估中点和损害水平的有害影响。此外,还确定了两个保护领域(AoPs)类别(人类健康和生态系统质量)和两个关注领域(AoCs)类别(碳相关损害和水相关损害)的潜在危害。研究结果表明,就 AoPs 而言,在 Moghan 和 Joveyn 地区,AIs 对人类健康的影响分别比 SHs 高 0.44% 和 4.32%。此外,在同一地区,人工影响对生态系统质量的影响分别比自然影响高出 7.97% 和 21.44%。与水有关的影响占两个农业活动方案环境影响总量的 90% 以上。在所有系统中,农场排放和氮肥是造成甜菜生产负面影响的主要因素,这强调了化肥管理在减轻环境破坏(尤其是与水相关的影响)方面的关键作用。
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引用次数: 0
Genome-Wide Identification of Sugar Beet (Beta vulgaris L.) MAPKKKs Gene Family and Their Expression in Response to Salt Stress 甜菜(Beta vulgaris L.)MAPKKs 基因家族的全基因组鉴定及其对盐胁迫的表达反应
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-25 DOI: 10.1007/s12355-024-01435-8
Xin-Miao Zhang, Guo-Qiang Wu, Ming Wei

Mitogen-activated protein kinase kinase kinases (MAPKKKs) are pivotal components of mitogen-activated protein kinase (MAPK) cascades and play important roles in many biological processes. To explore the biological function and genes expression patterns of the MAPKKK gene family in sugar beet (Beta vulgaris L.), a systematic analysis of BvMAPKKK was conducted. In the current study, a total of 40 BvMAPKKK genes are identified in the genome of sugar beet and can be categorized into three groups, ZIK, MEKK, and Raf with 9, 8, and 23 genes, respectively. The 37 BvMAPKKK genes were unevenly distributed on 9 chromosomes, while 3 BvMAPKKK genes were detected on unknown chromosomes. Members within the same group shared a similar gene structure and conserved motif. A lot of cis-acting regulatory elements related to plant growth and development and response to stresses were distributed in promoter regions of BvMAPKKKs. Moreover, these BvMAPKKKs displayed differential expression patterns in sugar beet tissues under salinity condition. The expression levels of the BvRaf30 and BvZIK8-1 in roots and BvRaf17 and BvZIK4 in shoots were markedly induced under salt conditions, which may be an indicator of potential roles in the response to salinity. The present work is the first systematic analysis of the BvMAPKKK gene family in sugar beet. These results could provide a solid foundation for further exploring functions of the MAPKKK genes in plant of responses to salt stress.

丝裂原活化蛋白激酶激酶(MAPKKK)是丝裂原活化蛋白激酶(MAPK)级联的关键组成部分,在许多生物过程中发挥着重要作用。为了探索甜菜(Beta vulgaris L.)中 MAPKKK 基因家族的生物学功能和基因表达模式,我们对 BvMAPKKK 进行了系统分析。目前的研究发现,甜菜基因组中共有 40 个 BvMAPKKK 基因,可分为三类:ZIK、MEKK 和 Raf,分别有 9、8 和 23 个基因。37 个 BvMAPKKK 基因不均匀地分布在 9 条染色体上,而在未知染色体上检测到 3 个 BvMAPKKK 基因。同一基因组内的成员具有相似的基因结构和保守的主题。在 BvMAPKKKs 的启动子区域分布着大量与植物生长发育和胁迫响应相关的顺式调控元件。此外,在盐度条件下,这些 BvMAPKKKs 在甜菜组织中表现出不同的表达模式。在盐分条件下,根中的 BvRaf30 和 BvZIK8-1 以及芽中的 BvRaf17 和 BvZIK4 的表达水平被明显诱导,这可能表明了它们在盐分反应中的潜在作用。本研究首次对甜菜中的 BvMAPKKK 基因家族进行了系统分析。这些结果可为进一步探索 MAPKKK 基因在植物盐胁迫响应中的功能奠定坚实的基础。
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引用次数: 0
Ameliorated Morpho-Physiological Response of Stevia Under the Influence of Variable LEDs Inside Plant Factory System 植物工厂系统中可变发光二极管影响下甜叶菊的形态-生理反应得到改善
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-24 DOI: 10.1007/s12355-024-01440-x
Sandeep Kumar Verma, Tsama Subrahmanyeswari, Saikat Gantait

The most prevalent light source for different plant species being cultivated under ex situ conditions is fluorescent lighting. Nevertheless, other light sources, such as light-emitting diodes (LEDs) in different colors, have shown to be more effective for such controlled conditions. In the present research, we have assessed the impact of variable light qualities on stevia (Stevia rebaudiana Bert.) seed germination and plant growth inside regulated environmental conditions. For this intent, red (RD), white (WH), blue (BL), and the different combinations of RD and BL LEDs were arranged in the plant factory system (PFS). Interestingly, it was found that the fresh weight (FW) and dry weight (DW) of leaves per plant (10.8 and 1.9 g) and FW and DW of roots per plant (2.7 and 0.4 g) measured to be maximum under RD:BL (1:1) exposure. The plants grown in PFS illuminated with RD:BL (8:2) LEDs showed maximum photosynthesis rate (8 μmol CO2/m2/s), chlorophyll a content (1.1 µg/ml), and stomata length and width in comparison to other LED exposures. Stomatal conductance and transpiration rate recorded maximum at 200 μmol/mol of CO2 concentration in plants raised under RD:BL (1:1) with 275 and 2.3 mmol H2O/m2/s, respectively. RuBisCO activity was recorded to be maximum under WH LED (0.17 µg/g). It was observed that a combination of RD and BL LEDs is more suitable for improved biomass, photosynthesis rate, chlorophyll contents, and stomatal conductance in the PFS than that of other individual light sources. The application of such biomass enhancement approach described in the present work might be advantageous for ameliorated steviol glycosides accumulation in stevia.

对于在异地条件下栽培的不同植物物种,最常用的光源是荧光灯。不过,其他光源,如不同颜色的发光二极管(LED),在这种受控条件下也显示出更高的效率。在本研究中,我们评估了不同光照质量对甜叶菊(Stevia rebaudiana Bert.)种子萌发和植物在受控环境条件下生长的影响。为此,我们在植物工厂系统(PFS)中布置了红光(RD)、白光(WH)、蓝光(BL)以及 RD 和 BL LED 的不同组合。有趣的是,在 RD:BL (1:1)照射下,每株植物的叶片鲜重(FW)和干重(DW)(分别为 10.8 克和 1.9 克)以及每株植物的根部鲜重(FW)和干重(DW)(分别为 2.7 克和 0.4 克)均达到最大值。使用 RD:BL (8:2) LED 照射的 PFS 中生长的植株的光合速率(8 μmol CO2/m2/s)、叶绿素 a 含量(1.1 µg/ml)、气孔长度和宽度与其他 LED 照射的植株相比均最大。在RD:BL(1:1)条件下,气孔导度和蒸腾速率分别在二氧化碳浓度为200 μmol/mol和2.3 mmol H2O/m2/s时达到最大值。在 WH LED(0.17 µg/g)条件下,RuBisCO 活性最高。据观察,与其他单个光源相比,RD 和 BL LED 的组合更适合提高 PFS 的生物量、光合作用速率、叶绿素含量和气孔导度。应用本研究中所述的生物量增强方法可能有利于改善甜叶菊中甜菊醇苷的积累。
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引用次数: 0
Comparative Analysis of Seven Endogenous Hormones in a Novel Dual-axillary Bud Mutant Genotype of Sugarcane 甘蔗新型双腋芽突变基因型中七种内源激素的比较分析
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-21 DOI: 10.1007/s12355-024-01430-z
Kamal Priyananda Wickramasinghe, Jun Mao, Chao-hua Xu, Xiu-qin Lin, Xu-juan Li, Wei Qin, Hong-bo Liu, Xin-long Liu, Faisal Mehdi, Pei-fang Zhao, Xin Lu

The sugarcane genotype YZ 07-86 is a distinct natural mutant with dual-axillary buds. Exploitation of this valuable trait in sugarcane breeding requires an understanding of the physiological mechanism of dual-axillary bud formation. This experiment determined the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellins (GA3 and GA4), and three cytokinins (CKs), namely isopentenyl adenosine (iPA), zeatin riboside (ZR), and dihydrozeatin riboside (DHZR), in the shoot apical meristem tissues of YZ 07-86 and its sister line YZ 07-87, which served as the control genotype. The field experiment followed a completely randomized design with three replications for each genotype. Sampling was done at 5-day intervals, starting 106 days after planting. The ELISA technique was used to measure the contents of endogenous hormones. The study revealed that the mean contents of IAA, GA3, GA4, DHZR, and iPA were not significantly different (P > 0.05) among the genotypes. Conversely, in YZ 07-86, the mean ZR content was significantly higher (P < 0.05), and the mean ABA content was significantly lower than in the control genotype. Moreover, the mean content ratios of ZR/IAA, ZR/ABA, ZR/GA3, and ZR/GA4 were also significantly higher in YZ 07-86. The study concludes that elevated ZR content and an increased ZR/ABA ratio directly contributes to dual-axillary bud formation in YZ 07-86, offering valuable insights for the advancement of sugarcane genotypes.

甘蔗基因型 YZ 07-86 是一种具有双腋芽的独特天然突变体。要在甘蔗育种中利用这一宝贵的性状,就必须了解双腋芽形成的生理机制。本实验测定了 YZ 07-86 及其姊妹品系 YZ 07-87 作为对照基因型的芽顶端分生组织中吲哚-3-乙酸(IAA)、脱落酸(ABA)、赤霉素(GA3 和 GA4)以及三种细胞分裂素(CKs),即异戊烯基腺苷(iPA)、玉米素核糖甙(ZR)和二氢玉米素核糖甙(DHZR)的含量。田间试验采用完全随机设计,每个基因型有三个重复。从播种后 106 天开始,每隔 5 天取样一次。采用 ELISA 技术测量内源激素的含量。研究表明,不同基因型的 IAA、GA3、GA4、DHZR 和 iPA 的平均含量差异不大(P > 0.05)。相反,在 YZ 07-86 中,ZR 的平均含量明显高于对照基因型(P < 0.05),而 ABA 的平均含量则明显低于对照基因型。此外,YZ 07-86 的 ZR/IAA、ZR/ABA、ZR/GA3 和 ZR/GA4 的平均含量比也明显较高。研究得出结论,ZR 含量的提高和 ZR/ABA 比率的增加直接促进了 YZ 07-86 双腋花芽的形成,为甘蔗基因型的改良提供了宝贵的启示。
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