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Appraisal on Energy Coefficients in Energetic Analysis of Agricultural Production System and Energetic Assessment on Sugarcane production 农业生产系统能能分析中的能能系数评价及甘蔗生产能能评价
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-14 DOI: 10.1007/s12355-025-01544-y
P. Subramanian, R. Mahendiran, B. Prabha, Gitanjali Jothiprakash, Ramesh Desikan, Bhargav Vinod Kumar, K. C. Pandey, Bhim Sen Pathak

Assessment on energy consumption pattern and energy analysis is carried out to identify and implement energy conservation measures in agriculture to reduce the cost of cultivation. Embodied energy of agricultural inputs and outputs is studied using energy coefficients. Considering the modernization in all the processes and systems, the necessity on revaluation of energy coefficients is understood for on-target and precise energy analysis in agriculture. Hence, the energy coefficients followed for the past few decades were appraised through appropriate calculations considering the amelioration of production and related processes. The rederived energy coefficients were evaluated through energy analysis in sugarcane cultivation. A preliminary and detailed energy audit on energy consumption in sugarcane cultivation was carried out in Orathur village, Villupuram district, Tamil Nadu, India. Various operational energy consumptions (land development, land preparation, planting, fertilizer application, irrigation, weeding, earthing-up, detrashing, harvesting, and transportation) and source-wise energy consumption (human power, fertilizer, mechanical energy, electrical energy, and fuel) were calculated by existing and rederived energy coefficients. From the energy audit, it is observed that irrigation was the most energy-consuming agricultural operation with 55 to 67% of total operational energy input. The other major energy sources in sugarcane cultivation were found to be seed (80—81%) and fertilizer (16%). These results show the effectiveness and relevance of rederived energy coefficients for assessing and optimizing energy inputs and output in sugarcane cultivation. Energy analysis in agricultural system helps to move agriculture closer toward sustainability by optimizing the energy usage and increased energy efficiency.

开展能源消费格局评价和能源分析,确定和实施农业节能措施,降低农业生产成本。利用能量系数对农业投入产出的蕴含能进行了研究。考虑到所有过程和系统的现代化,认识到对能量系数进行重估的必要性,以实现农业能源的准确、准确分析。因此,考虑到生产和相关过程的改进,通过适当的计算来评估过去几十年所遵循的能量系数。通过甘蔗栽培过程中的能量分析,对重新导出的能量系数进行了评价。在印度泰米尔纳德邦Villupuram地区的Orathur村对甘蔗种植的能源消耗进行了初步和详细的能源审计。各种操作能源消耗(土地开发、土地准备、种植、施肥、灌溉、除草、土化、破坏、收获和运输)和资源能源消耗(人力、肥料、机械能、电能和燃料)通过现有的和重新推导的能量系数来计算。从能源审计中可以看出,灌溉是最耗能的农业作业,占总作业能源投入的55 - 67%。甘蔗种植的其他主要能源为种子(80-81%)和肥料(16%)。这些结果表明,重新推导的能量系数在评估和优化甘蔗种植的能量投入和产出方面具有有效性和相关性。农业系统中的能源分析通过优化能源使用和提高能源效率,有助于农业向可持续发展迈进。
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引用次数: 0
Development and Optimization of an Integrated Disk-Blade Silage Device for Efficient Cane Top Collection and Processing 开发和优化用于高效蔗顶收集和加工的集成盘片青贮装置
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-14 DOI: 10.1007/s12355-025-01546-w
Peng Huo, Shaochun Ma, Baocheng Zhou, Wenzhi Li, Changyu Wang, Wei Liu, Sha Yang, Junhua Liu

To address the cane tops waste and collection machinery shortage, a disk-blade silage device was developed and integrated into a sugarcane harvester. This device enables simultaneous cutting, crushing, and ejection of cane tops. Guided by a theoretical model, key components were designed, and discrete element method simulation determined the optimal crushing speed as 1400 r/min and 12 blades as the best blade count. Compared with previous studies, this device features innovative structural design and working principle, which significantly improves the efficiency of cane tops processing. Field tests and simulations verified its effectiveness, achieving over 90% collection rate, 97% crushing efficiency, and an average throwing distance of 5.5 m, fulfilling the experimental parameters and design goals for silage processing.

为了解决甘蔗秸秆废料和收集机械短缺的问题,开发了一种圆盘叶片青贮装置,并将其集成到甘蔗收获机上。该装置可以同时切割,粉碎和弹出甘蔗顶部。在理论模型的指导下,设计了关键部件,通过离散元法仿真确定了最佳破碎速度为1400 r/min,最佳叶片数为12片。与以往的研究相比,该装置具有创新的结构设计和工作原理,显著提高了甘蔗顶加工效率。现场试验和仿真验证了其有效性,收集率达到90%以上,破碎效率达到97%,平均抛料距离达到5.5 m,达到青贮加工的实验参数和设计目标。
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引用次数: 0
Isolation and Identification of Plant Growth-Promoting Endophytes Bacteria Highly Effective in Suppressing Damping-off in Sugar Beet 甜菜高产促生长内生菌的分离与鉴定
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-12 DOI: 10.1007/s12355-025-01547-9
Bingchen Zou, Tai Li, Yonglong Liu, Zeyuan Cui, Lihua Yu, Gui Geng, Jiahui Liu, Yao Xu, Yuguang Wang

Sugar beet is an important sugar producing cash crop in the world with high economic value, but the crop faces a great economic loss every year due to damping-off. The concept of interactions produced between plant endophytic bacteria and host plants as an important biological control are gaining importance. In the present study, a strain of sugar beet standoff antagonist was obtained through plate standoff isolation and purification. Morphological observation, comparison of physiological and biochemical characteristics, and 16S rRNA gene sequences analysis were conducted for the classification and identification of the bacterial strain. The strain sb-13 was identified as Bacillus altitudinis. Through potting experiments, the growth of sugar beet inoculated with and without sb-13 was compared to analyze the effect of the endophytic bacteria on the growth of sugar beet, and it was found that the bacterium has the properties of promoting plant growth such as solubilizing phosphorus and dissolving potassium and enhancing the ability of soil enzyme activity. The experimental results showed that the growth of sugar beet inoculated with sb-13 was significantly better than that of the control group, and its physiological and biochemical indicators were significantly improved. In the anti-blight experiment, it was found that the bacterium antagonist had the ability to possess cellulose degradation and pectinase, and the incidence rate of sugar beet damping-off inoculated with sb-13 was significantly lower than that of the control group. This study provides a new idea for the biological control of sugar beet damping-off.

甜菜是世界上重要的制糖经济作物,具有很高的经济价值,但每年因受潮而遭受巨大的经济损失。植物内生细菌与寄主植物之间的相互作用作为一种重要的生物防治手段越来越受到重视。本研究通过平板对峙分离纯化获得了一株甜菜对峙拮抗剂。通过形态学观察、生理生化特征比较、16S rRNA基因序列分析对菌株进行分类鉴定。菌株sb-13鉴定为高原芽孢杆菌。通过盆栽试验,比较了接种和未接种sb-13对甜菜生长的影响,分析了内生细菌对甜菜生长的影响,发现该细菌具有溶磷、溶钾、提高土壤酶活性等促进植物生长的特性。实验结果表明,接种sb-13后甜菜的生长情况明显好于对照组,其生理生化指标均有显著改善。在抗枯萎病试验中,发现拮抗菌剂具有纤维素降解和果胶酶的能力,接种sb-13后的甜菜阻尼率显著低于对照组。本研究为甜菜萎蔫病的生物防治提供了新的思路。
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引用次数: 0
Recent Advances in the Adsorption and Mechanisms of Colored Substances in Sugarcane Juice: A Review 甘蔗汁中有色物质的吸附及其机理研究进展
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-10 DOI: 10.1007/s12355-025-01541-1
Kun Fang, Pei Li, Xiangrui Huang, Shengmei Gan, Yihan Li, Hanbing Wang

Historically, the production of white sugar from sugarcane has been marred by challenges like unsanitary conditions, excessive chemical use, suboptimal quality and yield, and high energy demands. These issues starkly contrast with modern sustainability principles, economy, and environmental consciousness. Green sugar clarification and decolorization techniques, especially adsorption-based method, have demonstrated superior efficiency in removing impurities and pigments while reducing chemical and energy requirements and simplifying operational procedures, making it a remarkably effective method. This review compares the advantages of adsorption process over conventional sugar production techniques with respect to color-forming impurities in sugarcane juice, and highlights recent applications of adsorption in the decolorization of sugarcane juice. In addition, this review explores the theoretical basis on adsorption and mass transfer with the objective of providing a comprehensive theoretical framework and detailed parameters for future color removal in sugarcane juice processing.

从历史上看,从甘蔗中生产白糖一直受到不卫生条件、过度使用化学品、质量和产量不理想以及高能量需求等挑战的影响。这些问题与现代可持续发展原则、经济和环境意识形成鲜明对比。绿糖澄清和脱色技术,特别是基于吸附的方法,在去除杂质和色素的同时,减少了化学和能量的需求,简化了操作程序,是一种非常有效的方法。本文综述了吸附法相对于传统制糖工艺处理甘蔗汁中显色杂质的优点,并重点介绍了吸附法在甘蔗汁脱色中的最新应用。此外,本文还对吸附传质的理论基础进行了探讨,旨在为今后甘蔗汁加工中脱色提供一个全面的理论框架和详细的参数。
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引用次数: 0
Identification of Drought Tolerance Indicators in Interspecific Hybrid Derivatives of Saccharum spp Using Membership Function Value of Drought Tolerance (MFVD) 利用抗旱隶属函数值(MFVD)鉴定糖蜜种间杂交衍生物的抗旱指标
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-04 DOI: 10.1007/s12355-025-01533-1
S. Alarmelu, S. Sheelamary, V. Anusheela, R. Arun Kumar, S. Vasantha, Huskur Kumaraswamy Mahadeva Swamy, A. Anna Durai

Drought is the foremost factor that severely affects growth, yield, and its yield components, and has a great impact on sugarcane productivity globally. Identification of drought-tolerant sugarcane cultivars is very important to mitigate climate-related risks to sustain productivity. Hence, it is critical to assess the pre-bred material with wide genetic diversity for drought tolerance for exploitation in breeding. Seventy-five hybrids with improved germplasm base of Saccharum officinarum, Saccharum spontaneum and Saccharum robustum were evaluated at ICAR-SBI, Coimbatore, under drought and normal conditions for yield and physiological traits, for their response under drought. The membership function value of drought tolerance (MFVD) of 19 traits was used as a comprehensive index for evaluation and selection of sugarcane drought-tolerant types. Mean value of all the traits except infrared and canopy temperature depression decreased under drought condition. Cane yield, single cane weight, cane height, soil plant analysis development, total chlorophyll, crop canopy, leaf area, total dry matter, and cane volume depicted > 25% decrease under drought. Leaf width had the highest broad sense heritability (0.69), followed by cane diameter (0.61). MFVD is strongly positively correlated with cane yield and its traits, infrared, leaf length, leaf width, total dry matter, and cane volume. Three genotypes, namely 07-776, 06-021, 20-1132, were highly drought tolerant, and 14-27, 01-807, 07-520, 13-455, 14-54, 04-409, 06-013, 13-644, 07-132 were drought tolerant using MFVD. Regression analysis on nine sensitive traits accounted for 93.1% of the total variation and may serve as indicators for screening sugarcane genotypes for drought tolerance. This new pre-breeding gene pool with high genetic diversity can be utilized in breeding climate resilient types of sugarcane.

干旱是严重影响甘蔗生长、产量及其组成部分的首要因素,对全球甘蔗生产力产生重大影响。鉴定耐旱甘蔗品种对于减轻气候相关风险以维持生产力非常重要。因此,对具有广泛抗旱遗传多样性的预配种材料进行鉴定,以便在育种中加以利用是至关重要的。在Coimbatore ICAR-SBI,对75个改良的甘蔗(Saccharum officinarum, Saccharum spontanum, Saccharum robustum)种质基础组合在干旱和正常条件下的产量和生理性状进行了评价。以19个性状的耐旱性隶属函数值(MFVD)作为评价和选择甘蔗耐旱品种的综合指标。干旱条件下,除红外和冠层温度降外,其他性状的平均值均降低。甘蔗产量、单根重、甘蔗高、土壤植物分析发育、总叶绿素、作物冠层、叶面积、总干物质和甘蔗体积在干旱下减少了25%。叶片宽度的广义遗传力最高(0.69),其次是茎粗(0.61)。MFVD与甘蔗产量及其性状、红外光谱、叶长、叶宽、总干物质和甘蔗体积呈极显著正相关。3个基因型07-776、06-021、20-1132具有高度耐旱性,14-27、01-807、07-520、13-455、14-54、04-409、06-013、13-644、07-132具有抗旱性。9个敏感性状的回归分析占总变异量的93.1%,可作为甘蔗抗旱基因型筛选的指标。这一具有高度遗传多样性的新型预育种基因库可用于甘蔗气候适应性品种的选育。
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引用次数: 0
Genotype-Specific Physiological Responses of Sugarcane to Potassium Deficiency: Integrating K Nutritional Efficiency, Photosynthesis-Related Parameters and Sugar Partitioning 甘蔗对缺钾的基因型特异性生理反应:综合钾营养效率、光合作用相关参数和糖分配
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-04 DOI: 10.1007/s12355-025-01538-w
Bruno José Zanchim, Elcio Ferreira Santos, Nikolas Souza Mateus, Nícolas Braga Casarin, André Rodrigues Reis, Francisco Scaglia Linhares, José Lavres

Potassium (K) nutritional requirements for sugarcane (Saccharum spp.) is relatively high. However, genotypes differ in their K uptake efficiency (KUpE) and use efficiency (KUtE), which shapes differences in plant growth. The study aimed to gain insight into the physiological responses of two sugarcane genotypes (SP90-3414 and SP83-2847) growth under two K concentrations in the nutrient solution (low K = 1.0 mmol L−1 and adequate K = 6.0 mmol L−1). Total leaf area, root growth and photosynthesis-related parameters were not changed in SP83-2847, regardless K supply, while for SP90-3414 under low K supply, these parameters were dramatically decreased. Under either low or adequate K supply, SP83-2847 genotype has higher KUpE and KUtE (K use efficient genotype) relative to SP90-3414, showing similar shoot dry matter yield and shoot K accumulation. Moreover, SP83-2847 plants grown under low K supply showed the highest accumulation of total soluble sugar and sucrose in leaves. The enhanced K accumulation in the shoot (leaves and stem) and sugar content (soluble sugar and sucrose) in the leaves of SP83-2847 cultivar is a physiological strategy to ease K-induced deficiency stress.

甘蔗(Saccharum spp.)对钾(K)的营养需求相对较高。然而,基因型在钾吸收效率(KUpE)和利用效率(KUtE)上存在差异,这决定了植物生长的差异。本研究旨在了解两种甘蔗基因型SP90-3414和SP83-2847在两种营养液(低K = 1.0 mmol L−1和高K = 6.0 mmol L−1)浓度下生长的生理反应。施钾不影响SP83-2847的总叶面积、根系生长和光合作用相关参数的变化,而施钾不足的SP90-3414则显著降低了这些参数。在低钾和充足钾供应下,SP83-2847基因型的KUpE和KUtE(钾利用效率基因型)均高于SP90-3414,表现出与SP90-3414相似的地上部干物质产量和地上部钾积累量。在低钾条件下,SP83-2847植株叶片中总可溶性糖和蔗糖积累量最高。SP83-2847提高茎部(叶和茎)钾积累量和叶片中糖(可溶性糖和蔗糖)含量是缓解缺钾胁迫的生理策略。
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引用次数: 0
Genetic Diversity Analysis of 89 Monogerm Maintainer Lines of Sugar Beet 89个甜菜单系保持系遗传多样性分析
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-04 DOI: 10.1007/s12355-025-01545-x
Jing-Jing Zhao, Lin-Lin Sun, Zhi Pi, Sheng-Nan Li, Ze-Dong Wu

Sugar beet is a kind of sugar crop, and sugar, as one of the strategic reserve materials, is of great significance to national development. China is not the origin of sugar beet, sugar beet monogerm germplasm resources are even less. In this study, we evaluated the genetic diversity of 89 genotypes of sugar beet monogerm maintainer line plants using 21 pairs of SSR primers and 34 pairs of InDel primers; a total of 251 pairs of alleles were obtained, with an average of 5.095 alleles per SSR locus and 4.1176 alleles per InDel locus. Based on 21 pairs of SSR markers determination, the gene flow (Nm) among individuals ranged from 0.0711 to 0.5419, with a mean value of 0.1858; based on 34 pairs of InDel markers determination, the gene flow (Nm) among individuals ranged from 0.0059 to 0.6953, with a mean value of 0.1406. Population structure analysis, cluster analysis, and principal coordinate analysis (PCoA) showed that the 89 sugar beet monogerm germplasm could be classified as two taxa PopI and PopII, which in turn could be divided into two subgroups (A and B). The genetic distances of the 89 sugar beet germplasm ranged from 0.1023 to 0.3930, with the genetic distance between germplasm No. 65 in subgroup A and No. 77 in subgroup B the furthest. Distance was the furthest, and these two germplasm were most suitable for breeding as parents. Analysis of molecular variance (AMOVA, P < 0.001) indicated that the major genetic variation occurred within individuals (98%), while the degree of genetic differentiation between populations (2%) was relatively low. This study provides a theoretical basis for selecting and breeding new varieties with high-quality of monogerm in sugar beet and increasing the number of monogerm maintainer lines in sugar beet.

甜菜是一种糖料作物,而糖作为战略储备物资之一,对国家发展具有重要意义。中国并不是甜菜的原产地,甜菜单基因种质资源更是少之又少。本研究利用21对SSR引物和34对InDel引物对89个基因型甜菜单性系保持系植株的遗传多样性进行了评价;共获得251对等位基因,平均每个SSR位点有5.095个等位基因,平均每个InDel位点有4.1176个等位基因。21对SSR标记检测结果表明,个体间基因流量(Nm)在0.0711 ~ 0.5419之间,平均值为0.1858;34对InDel标记检测结果显示,个体间基因流量(Nm)在0.0059 ~ 0.6953之间,平均值为0.1406。群体结构分析、聚类分析和主坐标分析(PCoA)表明,89份甜菜单性种质可划分为PopI和PopII两个类群,分别可划分为A和B两个亚群,遗传距离在0.1023 ~ 0.3930之间,其中A亚群65号和B亚群77号种质的遗传距离最远。距离最远,这两种种质最适合作为亲本进行育种。分子方差分析(AMOVA, P < 0.001)表明,主要的遗传变异发生在个体内部(98%),而群体间的遗传分化程度相对较低(2%)。本研究为选育优质甜菜单性系新品种和增加甜菜单性系保持系数量提供了理论依据。
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引用次数: 0
Genetic Improvement of Stevia (Stevia rebaudiana Bertoni): A Potential Calorie Free Natural Sweetener 甜菊糖的遗传改良:一种潜在的无热量天然甜味剂
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-02-04 DOI: 10.1007/s12355-025-01543-z
Shivanand D. Ainapur, T. N. Lakshmidevamma, B. N. Maruthi Prasad, H. R. Meghana, P Veeresh, Shivanand Koti

The increasing global demand for Stevia rebaudiana Bertoni, as a natural non-calorie sweetener has driven concentrated breeding efforts towards its genetic improvement for large-scale commercialization. Steviol glycosides responsible for sweetness in stevia leaves include stevioside, rebaudiosides and dulcoside. Inadequate genetic resources, characterization and the lack of high yielding, superior varieties pose major challenge in stevia production. Developing stevia varieties for high dry leaf yield, high steviol glycosides content particularly rebaudioside-A, optimum leaf-to-stem ratio, enhanced growth and photosynthetic activity, combating biotic and abiotic stresses is being targeted in genetic improvement programmes. Stevia exhibits considerable genetic diversity and in order to elucidate the genetics of steviol glycosides; the pattern of diversity has been assessed for achieving desired improvement. Recent advancements in biotechnological tools such as marker-assisted selection, genomics-assisted breeding, transcriptomics, metabolomics and genetic engineering have further accelerated the breeding progress. Marker-assisted and genomics-assisted breeding facilitate targeted improvements by identifying key genes linked to steviol glycosides biosynthesis. Genome sequencing of stevia revealed a 1416 Mb genome with over 80 per cent repetitive elements and transcriptomic analyses have identified critical glycosyltransferase genes involved in glycosylation, a critical process in steviol glycosides biosynthesis. Functional genomics and Agrobacterium-mediated transformation techniques have demonstrated the potential of metabolic engineering for enhancing steviol glycosides yield. This review highlights the progress made in stevia genetic improvement, emphasizing the integration of conventional breeding with advanced genomic tools to develop superior varieties and meet the rising demand for this natural sweetener.

甜菊糖作为一种天然的无热量甜味剂,全球对甜菊糖的需求日益增长,这推动了对其基因改良的集中育种努力,以实现大规模商业化。甜菊糖苷负责甜叶菊叶的甜味包括甜菊糖苷,雷鲍菊糖苷和杜糖苷。遗传资源不足、性状鉴定和缺乏高产优良品种是甜叶菊生产面临的主要挑战。遗传改良计划的目标是培育具有高干叶产量、高甜菊糖苷含量(特别是莱鲍迪苷a)、最佳叶柄比、增强生长和光合活性、抵抗生物和非生物胁迫的甜菊品种。甜菊糖具有相当大的遗传多样性,为了阐明甜菊糖苷的遗传;对多样性模式进行了评估,以实现预期的改善。标记辅助选择、基因组辅助育种、转录组学、代谢组学和基因工程等生物技术工具的最新进展进一步加速了育种进程。标记辅助和基因组辅助育种通过鉴定与甜菊糖苷生物合成相关的关键基因,促进了有针对性的改进。甜叶菊的基因组测序显示,其基因组长度为1416 Mb,重复元件超过80%,转录组学分析鉴定了参与糖基化的关键糖基转移酶基因,糖基化是甜叶菊糖苷生物合成的关键过程。功能基因组学和农杆菌介导的转化技术已经证明了代谢工程在提高甜菊醇糖苷产量方面的潜力。本文综述了甜菊糖遗传改良的进展,强调将传统育种与先进的基因组工具相结合,以开发优良品种,满足人们对这种天然甜味剂日益增长的需求。
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引用次数: 0
Optimization of an Enhanced Micropropagation Protocol of Stevia rebaudiana for Mass Production in an Indian Sub-humid Region 甜菊糖在印度半湿润地区大规模生产的强化微繁方案优化
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-01-27 DOI: 10.1007/s12355-025-01542-0
K. Abha Manohar, Kasireddy Sivasankarreddy, Gopal Shukla, Moumita Chakraborty, Bidhan Roy, Sumit Chakravarty

The present study was an attempt to optimize the regeneration protocols of Stevia rebaudiana (Bert.). Leaf and nodal explants were inoculated in MS medium supplemented with various plant growth regulators in different combinations and concentrations in order to attain direct and indirect regeneration. Among the treatments used, 2 mg/L of indole-3-acetic acid (IAA) + 1 mg/L of 6-Benzylaminopurine (BAP) displayed better results in stevia for callus induction when leaf and nodal explants were used, but in the case of nodal explants, it took more days for callus initiation compared to leaf segment. Among the treatments, 2.0 mg/L of IAA + 1.0 mg/L of BAP were found to be better for callus induction and subsequent plantlets regeneration (66.15%) from leaf explant of stevia. MS medium fortified with 0.5 mg/L of kinetin produced a high regeneration response (46.92%), the highest number of plantlets (40.67%) per explant, and the maximum number of roots (5.00) per plantlet in case of direct in vitro regeneration. While the MS medium fortified with 1.0 mg/L of BAP + 2.0 mg/L of IAA was found to be the best indirect in vitro regeneration of stevia plantlets from nodal explants concerning the highest regeneration response (68.10%), number of plantlets per explant and shoot length. MS medium added with 1.0 mg/L of BAP + 2.0 mg/L of IAA was reported to have the highest root regeneration ability (67.41%) and considerable length of roots for rooting of in vitro derived plantlets of stevia. The Soilrite was the best for acclimatizing in vitro regenerated plantlets of stevia. This is the first successful report on micropropagation in Stevia rebaudiana from sub-Himalayan region of West Bengal, India. A combination of IAA and BAP proved better for direct and indirect organogenesis. The Soilrite media was the best for acclimatization of in vitro regenerated plantlets with the highest (68.57%) survival rate in field conditions. Results of the present study will be useful for mass multiplication of Stevia rebaudiana using direct and indirect in vitro approaches. Moreover, direct in vitro shoot formation from leaves of Stevia rarely reported may open up possibilities of more successful large-scale cultivation of Stevia in the region and elsewhere.

本研究试图优化甜叶菊(Stevia rebaudiana (Bert.))的再生方案。将叶片和节的外植体接种到添加了不同组合和浓度的各种植物生长调节剂的 MS 培养基中,以实现直接和间接再生。在使用的处理中,2 毫克/升的吲哚-3-乙酸(IAA)+ 1 毫克/升的 6-苄基氨基嘌呤(BAP)对甜叶菊叶片和节的外植体诱导胼胝体有更好的效果,但与叶片相比,节的外植体需要更多的天数才能萌发胼胝体。在各种处理中,2.0 毫克/升 IAA + 1.0 毫克/升 BAP 对甜叶菊叶片外植体的胼胝体诱导和随后的植株再生(66.15%)效果较好。添加了 0.5 毫克/升酮素的 MS 培养基产生了较高的再生反应(46.92%),在直接离体再生的情况下,每个外植体的小植株数量最多(40.67%),每个小植株的根数量最多(5.00)。而添加了 1.0 毫克/升 BAP + 2.0 毫克/升 IAA 的 MS 培养基是从节的外植体间接再生甜叶菊小植株的最佳培养基,其再生反应(68.10%)、每个外植体的小植株数量和芽长均最高。据报道,添加了 1.0 毫克/升 BAP + 2.0 毫克/升 IAA 的 MS 培养基具有最高的根再生能力(67.41%)和相当长的根长,可用于甜叶菊离体衍生小植株的生根。在使甜叶菊离体再生小植株适应环境方面,Soilrite 的效果最好。这是印度西孟加拉邦次喜马拉雅地区首次成功报道甜叶菊的微繁殖。事实证明,IAA 和 BAP 的组合对直接和间接器官形成效果更好。在田间条件下,Soilrite 培养基对离体再生小植株的适应性最好,成活率最高(68.57%)。本研究的结果将有助于利用直接和间接体外方法大规模繁殖甜叶菊。此外,从甜叶菊叶片直接体外形成嫩枝的方法鲜有报道,这可能为该地区和其他地区更成功地大规模种植甜叶菊提供了可能性。
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引用次数: 0
Design of an Electrically Driven Sugarcane Seeding System Based on GNSS-RTK Receiver and SAPSO-LADRC Algorithm 基于GNSS-RTK接收机和SAPSO-LADRC算法的电动甘蔗播种系统设计
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-01-25 DOI: 10.1007/s12355-025-01540-2
Yang Li, Shang-ping Li, Chun-ming Wen, Kai-hua Li

In this study, the electrically driven sugarcane seeding system configures the desired plant spacing via the touchscreen human–computer interaction. The operating speed of the implement is acquired through the GNSS-RTK receiver in real-time during operation. In contrast, the instantaneous rotational speed of the motor is measured using a rotary encoder. Subsequently, the rotational speed of the sowing motor is regulated using a control system based on linear active disturbance rejection control. The parameters of this control system are optimized through the simulated annealing particle swarm optimization algorithm. A mechanism for monitoring misplaced sugarcane seeding is designed to detect seed misplacement instances and store the misplacement locations’ coordinates. The results of simulated seeding experiments indicate that, under various seeding operation speeds and plant spacing settings, the seeding motor’s theoretical and actual rotational speed discrepancy falls within the range of 4.03–6.61%. The average coefficient of variation for qualified plant spacing is 12.62%. The average error for seeding plant spacing is 8.44%. Compared to a similar type of ground wheel-driven sugarcane planting mechanism, the sugarcane planting system with electric drive exhibits an increase of 1.44% in the accuracy of planting spacing and a reduction of 6.72% in the average coefficient of variation for qualified plant spacing. The average positioning error for missing cane seeding is 84.11 mm, which can meet the sugarcane seedling shortage replanting operation requirements. The electric-driven sugarcane seeding system designed in this study overcomes the inflexibility of ground wheel-driven systems, effectively improving planting accuracy and flexibility. Additionally, it enables precise positioning of missed seeding spots, supporting efficient replanting operations. This study has reference significance for sugarcane precision planting technology.

在本研究中,电力驱动的甘蔗播种系统通过触摸屏人机交互配置所需的植株间距。在运行过程中,通过GNSS-RTK接收机实时获取装置的运行速度。相反,电机的瞬时转速是用旋转编码器测量的。随后,采用基于线性自抗扰控制的控制系统对播种电机的转速进行调节。采用模拟退火粒子群优化算法对控制系统参数进行优化。设计了一种甘蔗错种监测机制,用于检测错种实例并存储错种位置的坐标。模拟播种试验结果表明,在不同播种操作速度和播种间距设置下,播种电机的理论转速与实际转速之差在4.03 ~ 6.61%之间。株距合格的平均变异系数为12.62%。播种株距的平均误差为8.44%。与同类地轮驱动甘蔗种植机构相比,电驱动甘蔗种植系统的种植间距精度提高了1.44%,合格株距平均变异系数降低了6.72%。甘蔗缺苗平均定位误差为84.11 mm,可满足甘蔗缺苗补种作业要求。本研究设计的电动甘蔗播种系统克服了地面轮驱动系统的不灵活性,有效提高了播种精度和灵活性。此外,它可以精确定位错过的播种点,支持有效的补种操作。本研究对甘蔗精准种植技术具有参考意义。
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