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Enhancing Bioethanol Production from Banana Peel Biomass Using Photoactivated Nanoparticles: A Study of CoFe2O4 and g-C3N4 Composites 利用光活化纳米颗粒增强香蕉皮生物质生产生物乙醇:CoFe2O4和g-C3N4复合材料的研究
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-24 DOI: 10.1007/s12355-025-01578-2
Mona Maghraby, Samar Saeed, Ashraf Y. Elnaggar, Shams H. Abdel-Hafez, Yasser A. Attia

This study investigates the impact of various nanoparticles, specifically cobalt ferrite (CoFe2O4), graphitic carbon nitride (g-C3N4), and their composite (CoFe2O4/g-C3N4), on the ethanol production capabilities of Saccharomyces cerevisiae using banana peel biomass. Notably, the control group without any nanoparticle addition yielded a modest 11.157% bioethanol. However, the introduction of 100 ppm of CoFe2O4 nanoparticles significantly enhanced ethanol production, achieving 52.157%. Additionally, increasing the concentration of the CoFe2O4/g-C3N4 composite from 0 mg to 100 ppm resulted in a notable improvement, reaching an ethanol yield of 35.44%. Even with 100 ppm of g-C3N4 alone, ethanol production increased to 23%. To further optimize the fermentation process, the study also examined the effects of visible light irradiation on ethanol production, both in the presence and absence of these nanomaterials. The results revealed that light irradiation could stimulate bioethanol production by 15.44% compared to the control. When light was combined with nanoparticles, the stimulatory effects were even more pronounced, indicating that light-activated nanoparticles represent a promising strategy for enhancing ethanol yields. The findings highlight the potential of CoFe2O4 nanoparticles, particularly when photoactivated, to significantly elevate ethanol yields from banana peels. Furthermore, the study identifies the optimal concentration of the CoFe2O4/g-C3N4 composite as a viable pathway for sustainable bioethanol production. This research not only advances the understanding of nanomaterial applications in fermentation processes but also contributes to the development of more efficient and eco-friendly strategies for renewable fuel generation.

本研究考察了不同纳米颗粒,特别是铁酸钴(CoFe2O4)、石墨氮化碳(g-C3N4)及其复合材料(CoFe2O4/g-C3N4)对酿酒酵母利用香蕉皮生物质生产乙醇能力的影响。值得注意的是,没有添加任何纳米颗粒的对照组产生了适度的11.157%的生物乙醇。然而,引入100 ppm的CoFe2O4纳米颗粒可显著提高乙醇产量,达到52.157%。此外,将CoFe2O4/g-C3N4复合材料的浓度从0 mg增加到100 ppm,可显著提高乙醇收率,达到35.44%。即使仅使用100 ppm的g-C3N4,乙醇产量也增加到23%。为了进一步优化发酵过程,该研究还研究了可见光照射对乙醇生产的影响,包括存在和不存在这些纳米材料。结果表明,光照对乙醇产量的刺激作用较对照提高了15.44%。当光与纳米颗粒结合时,刺激效应更加明显,这表明光激活纳米颗粒代表了提高乙醇产量的有希望的策略。这一发现突出了CoFe2O4纳米颗粒的潜力,特别是在光活化的情况下,可以显著提高香蕉皮的乙醇产量。此外,该研究确定了CoFe2O4/g-C3N4复合材料的最佳浓度是可持续生物乙醇生产的可行途径。这项研究不仅促进了对纳米材料在发酵过程中的应用的理解,而且有助于开发更高效、更环保的可再生燃料生产策略。
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引用次数: 0
Utilization of Sugarcane Bagasse-Derived Bioadsorbents in a Packed Bed System for the Treatment of Fish Pond Effluent 蔗渣衍生生物吸附剂在填充床系统中处理鱼塘污水的研究
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-24 DOI: 10.1007/s12355-025-01575-5
Hussein Kehinde Okoro, Kingsley O. Iwuozor, Ibidun Isaac, Abel Egbemhenghe, Joseph Ighodalo Ehiguina, Ebuka C. Emenike, Aliru Olajide Mustapha, Caliphs Zvinowada, Catherine J. Ngila, Usman Isa, Adepoju Moronkola Idris, Valentine Ugochukwu Anyanwu, Adewale George Adeniyi

This research focused on exploring the efficacy of sugarcane bagasse-derived materials in a packed bed system for remediating aquaculture wastewater, with the goal of ensuring environmentally safe discharge. The bagasse fibres (SCB) were mercerized with sodium hydroxide (NaOH-SCB) and carbonized into biochar (SCB). Characterization techniques revealed significant chemical and physical changes in the modified bagasse, including changes in elemental composition, surface area, and thermal stability. The modified materials exhibited increased surface area and reduced pore size, and SCBB being the most thermally stable. The effect of contact times (30 min, followed by 1, 2, and 24 h) was studied on the treatment of fish pond effluent with bagasse-based materials. Physico-chemical parameters measured were temperature, pH, total dissolved solids, total suspended solids, electrical conductivity, dissolved oxygen, chemical oxygen demand, biological oxygen demand, and colour. The concentrations of metals (iron, magnesium, manganese, and copper) were also analysed. The treatment with the filter materials was effective in positively regulating various physico-chemical parameters of the effluent; however, it had a negative effect on the electrical conductivity and total dissolved solids. Among the materials, SCBB was the most effective for improving physico-chemical parameters, while NaOH-SCB was more effective for reducing the concentration of metals in the effluent. The optimum contact time for the treatment was observed to be 24 h for SCB, 2 h for NaOH-SCB, and either 30 min or one hour for SCBB. This study examines the effectiveness of sugarcane bagasse-based materials as a cost-effective and green alternative for industrial wastewater treatment.

本研究的重点是探索甘蔗甘蔗渣衍生材料在填充床系统中修复水产养殖废水的效果,以确保环境安全排放。用氢氧化钠(NaOH-SCB)对甘蔗渣纤维(SCB)进行丝光处理,炭化成生物炭(SCB)。表征技术揭示了改性甘蔗渣显著的化学和物理变化,包括元素组成、表面积和热稳定性的变化。改性后的材料比表面积增大,孔径减小,其中SCBB的热稳定性最好。研究了接触时间(30 min, 1、2、24 h)对甘蔗渣基材料处理鱼塘污水的影响。测量的理化参数包括温度、pH、总溶解固体、总悬浮固体、电导率、溶解氧、化学需氧量、生物需氧量和颜色。还分析了金属(铁、镁、锰和铜)的浓度。滤料处理能有效正向调节出水各项理化参数;然而,它对电导率和总溶解固形物有负面影响。其中,SCBB对改善出水理化参数效果最好,NaOH-SCB对降低出水金属浓度效果最好。最佳接触时间为SCB处理24 h, NaOH-SCB处理2 h, SCBB处理30 min或1 h。本研究考察了蔗渣基材料作为工业废水处理的成本效益和绿色替代品的有效性。
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引用次数: 0
Effect of Soil Compaction Caused by Manual and Mechanized Harvesting Management on Sugarcane Yield 人工和机械化采收管理对甘蔗土壤压实的影响
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-24 DOI: 10.1007/s12355-025-01571-9
Aldir Carpes Marques Filho, Murilo Battistuzzi Martins, Lucas Santos Santana, Arthur Gabriel Caldas Lopes, Renato Lustosa Sobrinho, Emerson F. C. Souza, Mohammad K. Okla, Ibrahim A. Alaraidh

Brazil is the world's largest producer of sugarcane, and mechanized management has increased the crop's productive efficiency. However, the intensity of machine traffic causes soil compaction, limiting the increase in productivity and longevity of crops. The harvesting operation compacts the soil as heavy machines move over the ground in inadequate moist conditions. This study evaluated soil compaction and sugarcane productivity in different productive areas with manual and mechanized harvesting practices over four years of harvest. Samples of soil resistance to penetration were taken after planting, harvesting, and from harvesting rows and between rows. The data were subjected to statistical analysis and Pearson's coefficient determination for soil penetration resistance and productivity variables over time. Resistance to soil penetration was observed in agricultural areas, regardless of management practices, with machinery primarily affecting the surface soil layers (0 to 0.3 m). The mechanized system, in the first year of the harvest, increased resistance to soil penetration by 270%, compared to 75% in the manual method. Manual harvesting management presented a cone index similar to a mechanized harvesting system, with a 14% increase in resistance to soil penetration. No significant differences in compaction were found between areas with manual and mechanized management at depths above 0.3 m. Productivity decreased linearly with cultivation time for both harvesting methods (r2 0.90 and 0.97—manual and mechanized), indicating that, independent of the harvesting model, sugarcane productivity tends to stabilize after the fourth year of production.

巴西是世界上最大的甘蔗生产国,机械化管理提高了作物的生产效率。然而,机器交通的强度导致土壤压实,限制了生产力的提高和作物的寿命。在潮湿条件不足的情况下,重型机械在地面上移动,收获作业使土壤变得密实。本研究在4年的采收过程中,采用人工和机械化方式对不同产区的土壤压实度和甘蔗生产力进行了评价。在播种后、收获后、收获行间和收获行间采集土壤抗渗透样本。对数据进行统计分析,并确定土壤渗透阻力和生产力变量随时间的皮尔逊系数。在农业地区,无论管理措施如何,都观察到土壤渗透阻力,机械主要影响表层土壤(0至0.3 m)。在收获的第一年,机械化系统增加了270%的抗土壤渗透能力,而手工方法增加了75%。人工采伐的锥体指数与机械化采伐相似,对土壤渗透的抵抗力提高了14%。在0.3 m以上的深度,手工和机械化管理区域的压实程度没有显著差异。两种采收方式的产量均随栽培时间线性下降(r2分别为0.90和0.97),表明与采收方式无关,甘蔗生产第四年后产量趋于稳定。
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引用次数: 0
Mathematical Modeling of Crop Water Production Functions for Sugar Beet 甜菜作物水分生产函数的数学建模
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-21 DOI: 10.1007/s12355-025-01572-8
Serhat Ayas

Crop water production functions, which describe the relationship between yield and water use, are of great importance in determining the economic value of irrigation, identifying different irrigation strategies and determining optimum irrigation levels. The main objective of this study was to evaluate the performance of Stewart, Jensen, Minhas, Blank and Rao functions in predicting root yield in sugar beet. Field studies were carried out in 2019 and 2020 in Bursa Yenişehir Vocational School Production Area. Water stress sensitivity indices of crop water production functions were determined using ET and yield values in the first year. Root yield values simulated with crop water production functions were compared with root yield values measured in the field in 2020. Sensitivity indices of sugar beet to water at four different growth stages were determined using five different crop water production models. Considering the sensitivity indices of sugar beet to water in four different growth periods, it was determined that the yield formation period (Y) was the most sensitive to water. Yield formation (Y) period was followed by vegetative (V) and establishment (E) periods. The least water-sensitive period of sugar beet was the ripening (R) period. Jensen and Minhas models were recommended when the sensitivity indices to water stress calculated for four different growth stages of sugar beet were compared.

作物水分生产函数描述了产量和水分利用之间的关系,对于确定灌溉的经济价值、确定不同的灌溉策略和确定最佳灌溉水平具有重要意义。本研究的主要目的是评价Stewart、Jensen、Minhas、Blank和Rao函数在甜菜根系产量预测中的表现。实地研究于2019年和2020年在布尔萨叶尼职业学校生产区进行。利用蒸散发和第一年的产量值确定了作物水分生产函数的水分胁迫敏感性指数。利用作物水分生产函数模拟的根系产量值与2020年田间实测的根系产量值进行了比较。采用5种不同的作物水分生产模型,测定了甜菜在4个不同生育期对水分的敏感性指数。综合4个不同生育期甜菜对水分的敏感性指标,确定产量形成期(Y)对水分最敏感。产量形成期(Y)之后是营养期(V)和成果期(E)。甜菜对水分最不敏感的时期是成熟期。在比较甜菜4个生育期水分胁迫敏感性指数时,推荐采用Jensen和Minhas模型。
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引用次数: 0
Utilization of Carbonized Sugarcane Leaves for Effective Encapsulation and Thermal Storage Enhancement of Phase Change Materials 利用炭化甘蔗叶片对相变材料进行有效包封和增强储热
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-21 DOI: 10.1007/s12355-025-01565-7
Zhi Li, Wentao Zhang, Ping Jiang

The application of phase change material (PCM) energy storage technology in the smart field has attracted a lot of attention. One of the important directions for the further development of energy storage technology is the development of shape-stabilized organic composite phase change materials. In this study, we developed a green stable composite phase change material (SCPCM) using carbonized sugarcane leaves (CSL) as a substrate. Sugarcane leaves is a thermolysis at 450 °C under three preparation conditions: aerobic unactivated (YONA), anaerobic unactivated (NONA), and anaerobic activated (NOYA), and subsequently combined with paraffin (PA) to produce paraffin/carbonized sugarcane leaves-stabilized composite phase change materials (PA/CSL SCPCM) for highly efficient thermal energy storage applications. All SCPCM had high thermal stability, good thermal properties, and good chemical compatibility of the composites, and the phase transition temperatures were controlled within the range of 24–34 °C. The support structure, specific surface area, active functional groups and high mesopore content of the activation-processed carbonized sugarcane leaves as the composite framework enhanced the capillary force of SCPCM adsorption on the CSL. The NOYACSL SCPCM had the largest latent heat storage capacity of 136.43 J/g. In contrast, the YONACSL SCPCM and NONACSL SCPCM had lower latent heat storage capacity of 108.9 J/g and 122.25 J/g. This study provides an economical and environmentally friendly method for the synthesis of shape-stabilized phase change materials based on biomass materials, which has potential applications in residual heat recovery and recycling.

相变材料(PCM)储能技术在智能领域的应用备受关注。储能技术进一步发展的重要方向之一是发展形状稳定的有机复合相变材料。本研究以炭化甘蔗叶片(CSL)为底物,制备了一种绿色稳定的复合相变材料(SCPCM)。甘蔗叶片在450℃下进行好氧非活化(YONA)、厌氧非活化(NONA)和厌氧活化(NOYA)三种制备条件下的热裂解,随后与石蜡(PA)结合,制备出石蜡/碳化甘蔗叶片稳定复合相变材料(PA/CSL SCPCM),用于高效储热应用。所有SCPCM均具有较高的热稳定性、良好的热性能和良好的复合材料化学相容性,相变温度控制在24-34℃范围内。活化处理的炭化甘蔗叶片作为复合骨架的支撑结构、比表面积、活性官能团和高介孔含量增强了SCPCM吸附在CSL上的毛细力。NOYACSL SCPCM潜热容量最大,为136.43 J/g。相比之下,YONACSL SCPCM和NONACSL SCPCM的潜热存储量较低,分别为108.9 J/g和122.25 J/g。本研究为基于生物质材料合成形状稳定相变材料提供了一种经济环保的方法,在余热回收和循环利用方面具有潜在的应用前景。
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引用次数: 0
Research Progress in Controlling Root Rot in Sugar Beet 甜菜根腐病防治研究进展
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-20 DOI: 10.1007/s12355-025-01563-9
Jing-Hao Li, Sheng-Nan Li, Zhi Pi, Ze-Dong Wu

Root rot poses a significant threat to sugar beet crops worldwide, making it a major concern in beet-growing regions. This study aimed to summarize the common causal organisms of sugar beet root rot and focus on identifying root rot pathogen populations, discovering germplasm resources discovery, and breeding resistance against major causal organisms. Additionally, the research progress on chemical and biological control methods for sugar beet root rot is discussed. In order to improve the resistance of sugar beet to root rot and obtain germplasm resources with high resistance to root rot, multidimensional collaboration strategy is recommended; implementation of pathogen population monitoring should be prioritized to identify predominant pathogenic fungi and elucidate their pathogenic mechanisms within cultivation zones, thereby providing scientific basis for targeted breeding of resistant cultivars; disease management systems require integration of synergistic applications between chemical and biological control agents to establish integrated management protocols; during breeding processes, molecular marker-assisted selection (MAS) technology should be incorporated to pinpoint disease resistance loci and optimize breeding efficiency; concurrent emphasis should be placed on evaluating the application potential of emerging biotechnological tools, which may overcome limitations inherent in conventional breeding methodologies.

根腐病对全世界的甜菜作物构成重大威胁,是甜菜种植区的一个主要问题。本研究旨在总结甜菜根腐病的常见致病生物,并着重于根腐病病原群体的鉴定、种质资源的发现以及对主要致病生物的抗性育种。并对甜菜根腐病化学和生物防治方法的研究进展进行了讨论。为提高甜菜对根腐病的抗性,获得高抗根腐病种质资源,建议采用多维度协同策略;重点开展病原菌种群监测,鉴定栽培区内优势病原菌,阐明其致病机制,为有针对性地选育抗病品种提供科学依据;疾病管理系统需要整合化学和生物防治剂之间的协同应用,以建立综合管理方案;在育种过程中,应结合分子标记辅助选择(MAS)技术,精确定位抗病位点,优化育种效率;同时应重视评价新兴生物技术工具的应用潜力,这些工具可能克服传统育种方法固有的局限性。
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引用次数: 0
Simulation and Experimental Validation of Modeling for Sugarcane Flexible Flake Materials 甘蔗柔性片状材料建模的仿真与实验验证
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-19 DOI: 10.1007/s12355-025-01562-w
Weiqing Li, Shaochun Ma, Baocheng Zhou, Wenzhi Li

Removal of impurities is one of the important parts of mechanized sugarcane harvesting. The main impurity (cane leaf) in the process of sugarcane machine harvesting belongs to flexible flake material. For the problem of less research on its construction simulation and lack of accurate simulation model parameters, this results in a large difference between the simulation effect and the actual operation in the equipment design, which to some extent limits the research on the design of the impurity removal device. In this paper, sugarcane material is used as the test material to carry out simulation and analysis research. After determining the intrinsic parameters of the sugarcane material through physical tests, a segmented flexible body model was established using EDEM software to calibrate the parameters of the sugarcane material. Stacking angle experiments and bending test methods were used to obtain its simulation model parameters. The coupled system model of billet–leaf—fan is constructed, and the reliability of the model is verified by the impurity rate, fan flow rate, cane leaf movement trajectory, and average speed. The experimental validation showed the simulation results and measurements at three fan speeds (1050 r/min, 1350 r/min, 1650 r/min): The average errors of the impurity rate, fan flow rate, and average cane leaf speed tests were 3.01%, 3.18%, and 4.21%, respectively; the trajectory of cane leaf movement was mainly ejected from the middle and upper middle of the fan outlet, which was the same as the test trajectory; the simulation results have high accuracy, proving that the model is relatively reliable. The model can theoretically solve the contradiction between the large size of sugarcane and impurities and the requirement that the grid size is not smaller than the particle size in the CFD-DEM coupling. It provides a reference for the coupling simulation of harvester debris removal device and the research of operation mechanism in the future.

除杂是甘蔗机械化收获的重要环节之一。甘蔗机械收获过程中的主要杂质(甘蔗叶)属于柔性片状物质。由于对其构造仿真研究较少,缺乏准确的仿真模型参数,导致在设备设计中仿真效果与实际运行存在较大差异,在一定程度上限制了除杂装置设计的研究。本文以甘蔗材料为试验材料进行模拟分析研究。通过物理试验确定甘蔗材料的内在参数后,利用EDEM软件建立了分段柔性体模型,对甘蔗材料的参数进行标定。采用堆垛角试验和弯曲试验等方法获得了其仿真模型参数。建立了坯料-叶片-风机耦合系统模型,并通过杂质率、风机流量、蔗叶运动轨迹、平均转速等参数验证了模型的可靠性。实验验证表明,在1050 r/min、1350 r/min、1650 r/min 3种风机转速下的模拟结果和测量结果:杂质率、风机转速和甘蔗叶片转速测试的平均误差分别为3.01%、3.18%和4.21%;甘蔗叶运动轨迹主要从风机出口的中部和中上方射出,与试验轨迹相同;仿真结果具有较高的精度,证明了该模型的可靠性。该模型从理论上解决了CFD-DEM耦合中甘蔗和杂质粒度大与网格尺寸不小于粒度要求的矛盾。为今后收割机排屑装置的耦合仿真及运行机构的研究提供参考。
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引用次数: 0
Nitrogen Monitoring and Sugar Yield Estimation Analysis of Sugar Beet Based on Multisource and Multi-temporal Remote Sensing Data 基于多源多时相遥感数据的甜菜氮素监测与糖产量估算分析
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-18 DOI: 10.1007/s12355-025-01555-9
Jingyun Wang, Xiaohang Hu, Xinjiu Dong, Shuo Liu, Yanli Li

This study aimed to explore the potential of multisource and multi-temporal UAV remote sensing data for sugar yield estimation and to investigate the relationship between different remote sensing features and nitrogen accumulation at various growth stages. UAV hyperspectral images, RGB images, and light detection and ranging (LiDAR) data were collected at different growth stages, and a comprehensive set of spectral, structural, and textural features reflecting the sugar beet canopy were extracted. Three machine learning algorithms, including multiple linear regression (MLR), random forest (RF), and support vector machine (SVM), were used to construct prediction models for nitrogen accumulation and sugar yield. The results showed the following. LiDAR features and textural features that characterize the canopy structure of sugar beet are essential for reflecting nitrogen accumulation, and LiDAR features play a key role in sugar yield prediction. For nitrogen accumulation prediction, the MLR model performed best during the rapid foliage growth period (R2 = 0.70, RMSE = 0.44 ). For sugar yield prediction, the MLR model, when combined with multi-temporal data, achieved the highest accuracy (R2 = 0.95, RMSE = 0.16), which was 21% higher than the best single-phase prediction result (sugar accumulation stage). The collaborative use of multisource remote sensing data significantly improved accuracy compared to single data sources, with nitrogen estimation accuracy increasing by 55% and sugar yield estimation accuracy increasing by 28%. These findings indicate that multisource remote sensing data can be used to diagnose nitrogen nutrition and predict sugar yield in sugar beet.

本研究旨在探索多源、多时段无人机遥感数据在糖产量估算中的潜力,探讨不同遥感特征与不同生长阶段氮素积累的关系。采集不同生长阶段的无人机高光谱图像、RGB图像和激光探测与测距(LiDAR)数据,提取反映甜菜冠层的光谱、结构和纹理等综合特征。采用多元线性回归(MLR)、随机森林(RF)和支持向量机(SVM)三种机器学习算法构建氮积累和糖产量预测模型。结果表明:表征甜菜冠层结构的激光雷达特征和纹理特征是反映氮素积累的必要条件,激光雷达特征在糖产量预测中起着关键作用。对于氮积累量的预测,MLR模型在叶片快速生长期表现最佳(R2 = 0.70, RMSE = 0.44)。在糖产量预测中,MLR模型与多时段数据相结合的预测精度最高(R2 = 0.95, RMSE = 0.16),比最佳单相预测结果(糖积累期)提高21%。与单一数据源相比,多源遥感数据的协同使用显著提高了精度,氮估算精度提高了55%,糖产量估算精度提高了28%。这些结果表明,多源遥感数据可用于甜菜氮素营养诊断和糖产量预测。
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引用次数: 0
Marker Assisted Selection for Rhizomania Resistance in Sugar Beet Genotypes 甜菜根瘤病抗性的标记辅助选择
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-14 DOI: 10.1007/s12355-025-01556-8
Peyman Norouzi

Rhizomania disease is the most important disease of sugar beet in Iran and some other parts of the world, and plays a significant role in decreasing sugar yield. The best approach to combat the disease is to use resistant cultivars. In order to use the disease resistance genes in breeding programs, it is necessary to tag these genes with molecular markers. In order to identify the rhizomania-resistant plants within 103 sugar beet genotypes including S1, S2 and F2 populations, a coupling-phase SCAR marker (named PN1) and a repulsion-phase RAPD marker (named PN3) linked to rhizomania resistance gene were used. Genomic DNA was extracted from the leaf samples taken from the single plants. In the next step, primers related to PN1 and PN3 markers were individually tested on some single plant DNAs by RAPD-PCR and Specific PCR methods. Amplified products were separated by gel electrophoresis, stained with ethidium bromide, observed using gel documentation device and scored according to the presence and absence of marker band. According to the marker data, percent dominant homozygous plants were found to be between 0 to 92%. The O-types numbers 1025, 1036, 1011, 201–20 and 201–25 were found to be the most disease resistant genotypes. Therefore, it seems PN3 marker, after converting to SCAR specific marker, along with PN1 marker can be utilized in one duplex PCR reaction to identify each of the three genotypes (Rz1Rz1, Rz1rz1 and rz1rz1) and screen rhizomania resistant breeding lines and populations originated from Rz1 source.

根茎病是伊朗和世界上一些地区甜菜最重要的病害,是导致糖产量下降的重要原因。对付这种病的最好办法是使用抗病品种。为了在育种中利用抗病基因,有必要对这些基因进行分子标记。为了鉴定103个甜菜基因型(S1、S2和F2群体)的根瘤病抗性植株,采用了根瘤病抗性基因连锁的偶联期SCAR标记(PN1)和排斥期RAPD标记(PN3)。从单株植物的叶片样本中提取基因组DNA。下一步,利用RAPD-PCR和Specific PCR方法分别对PN1和PN3标记相关引物在部分单株dna上进行检测。扩增产物经凝胶电泳分离,溴化乙锭染色,用凝胶记录仪观察,根据有无标记带进行评分。根据标记数据,显性纯合子株数在0 ~ 92%之间。1025、1036、1011、201-20和201-25是抗病能力最强的o型基因型。因此,PN3标记在转化为SCAR特异性标记后,与PN1标记一起在一次双链PCR反应中可以分别鉴定Rz1Rz1、Rz1Rz1和Rz1Rz1三种基因型,并筛选来自Rz1源的根瘤病抗性育种品系和群体。
{"title":"Marker Assisted Selection for Rhizomania Resistance in Sugar Beet Genotypes","authors":"Peyman Norouzi","doi":"10.1007/s12355-025-01556-8","DOIUrl":"10.1007/s12355-025-01556-8","url":null,"abstract":"<div><p>Rhizomania disease is the most important disease of sugar beet in Iran and some other parts of the world, and plays a significant role in decreasing sugar yield. The best approach to combat the disease is to use resistant cultivars. In order to use the disease resistance genes in breeding programs, it is necessary to tag these genes with molecular markers. In order to identify the rhizomania-resistant plants within 103 sugar beet genotypes including S<sub>1</sub>, S<sub>2</sub> and F<sub>2</sub> populations, a coupling-phase SCAR marker (named PN1) and a repulsion-phase RAPD marker (named PN3) linked to rhizomania resistance gene were used. Genomic DNA was extracted from the leaf samples taken from the single plants. In the next step, primers related to PN1 and PN3 markers were individually tested on some single plant DNAs by RAPD-PCR and Specific PCR methods. Amplified products were separated by gel electrophoresis, stained with ethidium bromide, observed using gel documentation device and scored according to the presence and absence of marker band. According to the marker data, percent dominant homozygous plants were found to be between 0 to 92%. The O-types numbers 1025, 1036, 1011, 201–20 and 201–25 were found to be the most disease resistant genotypes. Therefore, it seems PN3 marker, after converting to SCAR specific marker, along with PN1 marker can be utilized in one duplex PCR reaction to identify each of the three genotypes (<i>Rz</i><sub><i>1</i></sub><i>Rz</i><sub><i>1</i></sub>, <i>Rz</i><sub><i>1</i></sub><i>rz</i><sub><i>1</i></sub> and <i>rz</i><sub><i>1</i></sub><i>rz</i><sub><i>1</i></sub>) and screen rhizomania resistant breeding lines and populations originated from <i>Rz</i><sub><i>1</i></sub> source.</p></div>","PeriodicalId":781,"journal":{"name":"Sugar Tech","volume":"27 4","pages":"1081 - 1088"},"PeriodicalIF":2.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145165632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing Sugar Crop Resilience to Abiotic Stress Using CRISPR/Cas Tools 利用CRISPR/Cas工具增强糖作物对非生物胁迫的抗性
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-03-13 DOI: 10.1007/s12355-025-01561-x
Himanshu Pandey, Varucha Misra, A. K. Mall, Avinash Sharma, V. Edwin Hillary, Stanislaus Antony Ceasar

Sugar crops such as sugarcane, sugar beet, and sweet sorghum are vital to global agriculture, serving as key sources of sweeteners, biofuels, and industrial raw materials. However, their productivity and sustainability are increasingly threatened by abiotic stresses, including drought, salinity, heat, chilling, and heavy metal toxicity. These stresses affect complex polygenic traits, necessitating precise and efficient genetic interventions for developing stress-resilient cultivars. This review aims to explore the potential of CRISPR/Cas genome editing technologies, including CRISPR/Cas9, CRISPRa, CRISPRi, prime editing, and base editing, in enhancing abiotic stress tolerance in sugar crops. These advanced genome editing tools facilitate targeted modifications, enabling gain-of-function mutations and regulatory network studies to accelerate genetic improvement. A particular focus is given to transcription factor families such as DREB, NAC, and WRKY, which regulate key genes associated with osmoprotection, stomatal regulation, and stress signaling pathways. Furthermore, strategies for generating transgene-free edited plants, including Preassembled CRISPR/Cas9 Ribonucleoproteins-Based Genome Editing, the CASE toolkit, Hi-Edit technology, and Transgene Killer CRISPR technology, are also emphasized. By integrating CRISPR-based strategies with conventional breeding, this review article aims to provide a framework for developing resilient sugar crop varieties capable of withstanding environmental challenges. Additionally, regulatory considerations for genome-edited crops are discussed to highlight the implications for commercial adoption. The insights will contribute to sustainable sugar crop production by leveraging precise genome editing approaches to enhance stress tolerance.

甘蔗、甜菜和甜高粱等糖作物对全球农业至关重要,是甜味剂、生物燃料和工业原料的主要来源。然而,它们的生产力和可持续性越来越受到非生物胁迫的威胁,包括干旱、盐、热、冷和重金属毒性。这些胁迫会影响复杂的多基因性状,因此需要精确和有效的遗传干预来培育抗胁迫品种。本文旨在探讨CRISPR/Cas基因组编辑技术,包括CRISPR/Cas9、CRISPRa、CRISPRi、引体编辑和碱基编辑在提高糖作物非生物胁迫耐受性方面的潜力。这些先进的基因组编辑工具促进了靶向修饰,使功能获得突变和调控网络研究能够加速遗传改进。特别关注转录因子家族,如DREB、NAC和WRKY,它们调节与渗透保护、气孔调节和应激信号通路相关的关键基因。此外,还强调了生成无转基因编辑植物的策略,包括基于预组装CRISPR/Cas9核糖核蛋白的基因组编辑、CASE工具包、Hi-Edit技术和转基因杀手CRISPR技术。通过将基于crispr的策略与传统育种相结合,这篇综述文章旨在为开发能够抵御环境挑战的抗灾糖作物品种提供一个框架。此外,还讨论了对基因组编辑作物的监管考虑,以突出其对商业应用的影响。这些见解将通过利用精确的基因组编辑方法来提高抗逆性,为可持续的糖作物生产做出贡献。
{"title":"Enhancing Sugar Crop Resilience to Abiotic Stress Using CRISPR/Cas Tools","authors":"Himanshu Pandey,&nbsp;Varucha Misra,&nbsp;A. K. Mall,&nbsp;Avinash Sharma,&nbsp;V. Edwin Hillary,&nbsp;Stanislaus Antony Ceasar","doi":"10.1007/s12355-025-01561-x","DOIUrl":"10.1007/s12355-025-01561-x","url":null,"abstract":"<div><p>Sugar crops such as sugarcane, sugar beet, and sweet sorghum are vital to global agriculture, serving as key sources of sweeteners, biofuels, and industrial raw materials. However, their productivity and sustainability are increasingly threatened by abiotic stresses, including drought, salinity, heat, chilling, and heavy metal toxicity. These stresses affect complex polygenic traits, necessitating precise and efficient genetic interventions for developing stress-resilient cultivars. This review aims to explore the potential of CRISPR/Cas genome editing technologies, including CRISPR/Cas9, CRISPRa, CRISPRi, prime editing, and base editing, in enhancing abiotic stress tolerance in sugar crops. These advanced genome editing tools facilitate targeted modifications, enabling gain-of-function mutations and regulatory network studies to accelerate genetic improvement. A particular focus is given to transcription factor families such as DREB, NAC, and WRKY, which regulate key genes associated with osmoprotection, stomatal regulation, and stress signaling pathways. Furthermore, strategies for generating transgene-free edited plants, including Preassembled CRISPR/Cas9 Ribonucleoproteins-Based Genome Editing, the CASE toolkit, Hi-Edit technology, and Transgene Killer CRISPR technology, are also emphasized. By integrating CRISPR-based strategies with conventional breeding, this review article aims to provide a framework for developing resilient sugar crop varieties capable of withstanding environmental challenges. Additionally, regulatory considerations for genome-edited crops are discussed to highlight the implications for commercial adoption. The insights will contribute to sustainable sugar crop production by leveraging precise genome editing approaches to enhance stress tolerance.</p></div>","PeriodicalId":781,"journal":{"name":"Sugar Tech","volume":"27 4","pages":"985 - 1002"},"PeriodicalIF":2.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145164762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Sugar Tech
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