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Four-Year Field Study: Increased Yields in Soils Amended with Biochar from Sugarcane Residues 四年田野研究:用甘蔗残茬生物炭改良土壤可提高产量
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-23 DOI: 10.1007/s12355-025-01600-7
Isabel M. Lima, Paul M. White Jr.

Surplus sugarcane bagasse and leaf field residue remain untapped resources associated with sugarcane processing and harvesting. Their pyrolysis produces biochar which can be land applied to improve soil health and crop performance. Biochar produced from field residue and sugarcane bagasse was applied once at either 0.80 Mg ha−1 (LB1, BB1) or 1.60 Mg ha−1 (LB2, BB2) to sugarcane, as single amendment or in combination with fly ash at 0.63 or 1.26 Mg ha−1. Through their high content in organic matter, fixed carbon and H:C ratio (68.8%, 64%, 0.48 and 71.1%, 60%, 0.46 for BB and LB, respectively), biochar helped build soil organic carbon and increased the permanent carbon pool in the soil. HoCP 96-540 cane yield, theoretical recoverable sucrose, and sugar yield were measured and compared across treatments for 4 years. Biochar treatments led to improvements to crop performance, albeit some were not significantly different than the control. No reductions in sugar yield were observed for FA additions. Best results were determined for field application of LB1 followed by BB2, specifically with 0.90 and 0.88 kg/stalk, cane yield of 85.8 and 83.3 Mg ha−1, sucrose yield of 8647 and 8210 kg ha−1, respectively, when compared to control at 0.78 kg/stalk, cane yield of 68.3 Mg ha−1, and sucrose yield of 6566 kg ha−1. Considering the cumulative sugar yield over 4 years, additional income can be realized from amending soil with biochar. Best outcome resulted in estimated sugar profits up to additional $6837 ha−1 for LB1 (raw sugar at $0.82 kg−1).

剩余甘蔗渣和田间叶渣是与甘蔗加工和收获有关的未开发资源。它们的热解产生生物炭,可用于土地,以改善土壤健康和作物性能。由田间残渣和甘蔗渣生产的生物炭以0.80 Mg ha - 1 (LB1, BB1)或1.60 Mg ha - 1 (LB2, BB2)的剂量一次性施用于甘蔗,作为单一改良剂或与0.63或1.26 Mg ha - 1的粉煤灰混合施用。生物炭具有较高的有机质、固定碳含量和H:C比值(BB和LB分别为68.8%、64%、0.48和71.1%、60%、0.46),有助于构建土壤有机碳,增加土壤永久碳库。对HoCP 96-540甘蔗产量、理论可采蔗糖和蔗糖产量进行了4年的测定和比较。生物炭处理导致作物生产性能的改善,尽管有些与对照没有显著差异。添加FA后糖产量没有降低。田间施用LB1和BB2的效果最好,分别为0.90和0.88 kg/茎,甘蔗产量分别为85.8和83.3 Mg ha - 1,蔗糖产量分别为8647和8210 kg ha - 1,而对照为0.78 kg/茎,甘蔗产量为68.3 Mg ha - 1,蔗糖产量为6566 kg ha - 1。考虑到4年的累积产糖量,利用生物炭改良土壤可以实现额外的收入。最佳结果导致LB1(原糖0.82 kg - 1)的糖利润估计高达6837 ha - 1。
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引用次数: 0
Evaluation of Optimal Timing for Replacement of Self-Propelled Sugar Cane Harvesters 自走式甘蔗收割机更换最佳时机的评价
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-20 DOI: 10.1007/s12355-025-01625-y
Erick Henrique Romero, Priscila Fernanda Pereira Barbosa, Paulo Alexandre Monteiro de Figueiredo, Sérgio Bispo Ramos, Ronaldo da Silva Viana

The sugarcane harvester plays a fundamental role in determining the quality of the raw material destined for industrial processing; however, its management is complex due to high acquisition and operating expenses. This study aimed to determine the optimal replacement point for sugarcane harvesters by applying established methodologies adapted to agricultural conditions. The analysis was based on principal component analysis, with evaluations focused on cost, production, availability, hours worked, and harvest year for each machine. The results indicated that, for the JD 3520 harvester, the ideal replacement point occurs at the end of year 3 or the beginning of year 4, when operational costs increase sharply and availability drops significantly, rendering the equipment financially unviable in relation to its productivity. In the case of the JD CH570 harvester, replacement should be considered at the end of year 3, as the costs per ton (R$/t) and per hour (R$/h) begin to rise notably, while operational availability also declines. It is concluded that identifying the optimal replacement moment—by balancing operational, maintenance, and financial factors—is essential for strategic decision-making. This enables planned fleet renewal, aligned with market conditions and supported by a robust database, ensuring greater reliability and confidence for stakeholders.

甘蔗收割机在确定用于工业加工的原料的质量方面起着重要作用;然而,由于高昂的收购和运营费用,其管理非常复杂。本研究旨在通过应用适应农业条件的既定方法来确定甘蔗收割机的最佳替换点。分析基于主成分分析,评估集中于每台机器的成本、产量、可用性、工作时间和收获年份。结果表明,对于JD 3520收割机,理想的更换点发生在第3年底或第4年初,此时运营成本急剧增加,可用性显着下降,使设备相对于其生产力在财务上不可行。以JD CH570收割机为例,应在第三年底考虑更换,因为每吨(R$/t)和每小时(R$/h)的成本开始显著上升,同时操作可用性也下降。结论是,通过平衡运营、维护和财务因素,确定最佳的替换时机对战略决策至关重要。这使得机队更新计划得以实现,与市场状况保持一致,并得到强大数据库的支持,确保了利益相关者的更高可靠性和信心。
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引用次数: 0
Determination of Critical Period of Weed Control in Tropical Sugar Beet: First Step to Formulate Sustainable Weed Management Strategy 确定热带甜菜杂草控制关键期:制定可持续杂草管理战略的第一步
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-18 DOI: 10.1007/s12355-025-01624-z
Anjon Mallick, Mahfuza Begum, Sabuj Chandra Roy, Md. Parvez Anwar, Md. Harun Rashid, Swapan Kumar Paul

Weed interference is one of the most critical factors limiting sugar beet productivity, especially under tropical conditions. Identifying the critical period of weed control (CPWC) can optimize weed management and reduce yield loss. A field experiment was conducted to investigate the performance of tropical sugar beet as influenced by weed interference period and assess the CPWC in 2024 at Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, Bangladesh. Twelve distinct weed interference periods were investigated along with maintaining complete weed free and unweeded for whole growing periods. The experimental results showed that weed interference periods significantly influenced the growth, yield and quality of tropical sugar beet. Longer durations of weed interference reduced root length, root girth, shoot yield and root yield of sugar beet. Conversely, extended weed-free periods increased these yield components. Season long unweeded plots experienced approximately 94% yield loss compared to season long weed-free plots. CPWC was found between 133 and 1121 growing degree of days (GDD) equivalent to 6–74 days after emergence (DAE) based on 5% acceptable yield loss. In can be concluded that establishing and maintaining a weed-free environment around the first ten weeks after emergence of sugar beet is crucial to overcome yield loss at 5% threshold level. Weed control cost can also be minimized by optimizing the timing of weed management strategies. These findings are based on specific regional conditions; thus, broader environmental research would enhance their applicability.

杂草干扰是限制甜菜产量的最关键因素之一,特别是在热带条件下。确定杂草控制关键期可以优化杂草管理,减少产量损失。在位于孟加拉国迈门辛格的孟加拉国农业大学农学田间实验室进行了田间试验,研究了杂草干扰期对热带甜菜生产性能的影响,并对2024年CPWC进行了评估。研究了12个不同的杂草干扰期,并在整个生育期保持完全无杂草和不除草。试验结果表明,杂草干扰期对热带甜菜的生长、产量和品质有显著影响。杂草干扰时间越长,甜菜根长、根围、茎部产量和根产量均降低。相反,延长无草期增加了这些产量成分。与无杂草地块相比,长时间未除草地块的产量损失约为94%。按5%可接受产量损失计算,CPWC为133 ~ 1121生长期(GDD),相当于出苗后6 ~ 74天。综上所述,在甜菜出苗后10周左右建立和维持无杂草环境对克服5%阈值水平的产量损失至关重要。杂草控制成本也可以通过优化杂草管理策略的时机来最小化。这些发现是基于特定的区域条件;因此,更广泛的环境研究将增强它们的适用性。
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引用次数: 0
Computational Analysis of microRNAs in Sweet Sorghum (Sorghum bicolor) Infected by Sorghum Mosaic Virus 感染高粱花叶病毒的甜高粱(高粱双色)microrna的计算分析
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-17 DOI: 10.1007/s12355-025-01622-1
Mohamad Ghodoum Parizipour, Aminallah Tahmasebi, Amir Ghaffar Shahriari

Sweet sorghum can be negatively affected by sorghum mosaic virus (SrMV). The application of microRNAs (miRNAs) is a novel approach for antiviral defense in host plants. Herein, an in silico analysis was performed, and the molecular interaction of SrMV and sorghum-encoded miRNAs was determined through the identification of the host miRNAs which can target the viral RNA leading to possible antiviral defense. Moreover, the molecular pathways activated by the miRNA-targeted genes were characterized. The results showed that 24 miRNA targets are located on the viral genome inhibiting the sequences by cleavage and translational mechanisms. Also, the putative structures and entropic properties of the miRNAs were determined. Moreover, the miRNAs could target five open reading frames (P1, HC-Pro, NIa, NIb, and CP) located on the viral genome. Interestingly, a non-coding region of the viral genome, i.e., untranslated regions at the 5’ terminus, was targeted by two miRNAs. Twelve host genes involved in biotic stress were targeted by the miRNAs. Gene ontology (GO) analysis showed that 4123 host genes were targeted by the miRNAs. GO enrichment exhibited that these genes are involved in 307,196 biochemical pathways in the host. Thirty biochemical pathways were found to be significantly enriched among which were defense-related pathways including kinase activity, phosphorylation, ATP binding, coiled-coil, and nucleotide binding. It can be concluded that the sorghum-originated miRNAs can target different genomic regions of SrMV and regulate the defense-related host genes as well. These data can be used in the production of resistant sorghum plants challenged by SrMV.

高粱花叶病毒(sorghum mosaic virus, SrMV)对甜高粱有不良影响。microRNAs (miRNAs)的应用为宿主植物的抗病毒防御提供了一种新的途径。本文进行了计算机分析,并通过鉴定可靶向病毒RNA的宿主mirna来确定SrMV与高粱编码的mirna的分子相互作用,从而导致可能的抗病毒防御。此外,表征了mirna靶向基因激活的分子途径。结果表明,24个miRNA靶点位于病毒基因组上,通过切割和翻译机制抑制这些序列。此外,还确定了mirna的推定结构和熵性质。此外,mirna可以靶向位于病毒基因组上的五个开放阅读框(P1, HC-Pro, NIa, NIb和CP)。有趣的是,病毒基因组的非编码区,即5 '端的非翻译区,被两个mirna靶向。这些mirna靶向了12个参与生物胁迫的宿主基因。基因本体(GO)分析显示,4123个宿主基因被mirna靶向。氧化石墨烯富集表明,这些基因参与了宿主体内307,196条生化途径。发现30条生化途径显著富集,其中防御相关的途径包括激酶活性、磷酸化、ATP结合、卷曲线圈和核苷酸结合。由此可见,高粱来源的mirna可以靶向SrMV的不同基因组区域,并调控与防御相关的宿主基因。这些数据可用于生产抗SrMV的高粱植株。
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引用次数: 0
Revisited Fertilizer Doses for Sugarcane in North Coastal Andhra Pradesh for Replenished Soil and Crop Productivity 重新考察了安得拉邦北部沿海地区甘蔗的肥料剂量,以补充土壤和作物生产力
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-16 DOI: 10.1007/s12355-025-01623-0
Ch. S. Ramalakshmi, T. Sreelatha, A. Sireesha, M. Bharathalakshmi

A field experiment was conducted consecutively for two plant crops of sugarcane at Regional Agricultural Research Station, Anakapalle, Andhra Pradesh and one year on-farm testing at farmers field with a variety 2001A 63 from 2016 to 2019 to revise the fertilizer doses for sugarcane plant crop. Among different fertilizer doses, significantly superior cane yields (81.48 and 86.18 t ha−1) and sugar yields (11.93 and 12.63 t ha−1) were recorded in T6, i.e., 168–100–120 kg NPK ha−1 + 25% recommended dose of Zn, S and B (12.5 + 7.5 + 2.5 kg ha−1) in M1 (without manure) and M2 (with manure, i.e., Farm Yard Manure @ 10 t ha−1) during first year. Cane and sugar yields were further increased in second year (cane yields of 86.48 and 89.68 t ha−1 in M1 and M2, respectively). Cane yield improvement to an extent of 12% was observed with refined fertilizer dose over existing recommendation (5.02 to 6.21 t ha−1) yield improvement in refined or revisited nutrient package over existing nutrient package, like wise sugar yield improvement of 0.41 to 1.08 t ha−1 was observed in refined recommendation over existing recommendation during first year and second year, respectively. Results clearly showed that, application of 168 kg nitrogen, 100 kg phosphorus, 120 kg potassium and basal application of micronutrients, i.e., 12.5 + 7.5 + 2.5 kg ha−1 of Zn, S and B along with farmyard manure @ 10 t ha−1 recorded significantly superior cane and sugar yields along with sustenance of soil fertility and cane juice quality for sugarcane plant crop in North Coastal Andhra Pradesh.

2016 - 2019年,在安得拉邦Anakapalle地区农业研究站对甘蔗两种植物作物进行了连续大田试验,并在农民田间进行了为期一年的2001 - 63品种试验,以修订甘蔗植物作物的肥料用量。在不同的施肥剂量中,第一年的甘蔗产量(81.48和86.18 t ha - 1)和糖产量(11.93和12.63 t ha - 1)在T6,即168-100-120 kg NPK ha - 1 + 25%推荐剂量的Zn, S和B (12.5 + 7.5 + 2.5 kg ha - 1)在M1(不施肥)和M2(有肥料,即农场厩肥@ 10 t ha - 1)中显著提高。第二年甘蔗和糖产量进一步提高(M1和M2甘蔗产量分别为86.48和89.68吨/公顷)。精肥用量比现有推荐量(5.02 ~ 6.21 t ha - 1)提高了12%的甘蔗产量;精肥或改良营养包比现有营养包增产,例如,在第一年和第二年,精肥推荐比现有推荐量分别增产0.41 ~ 1.08 t ha - 1。结果清楚地表明,在安得拉邦北部沿海地区,施用168 kg氮、100 kg磷、120 kg钾和基础施用微量营养素,即12.5 + 7.5 + 2.5 kg Zn、S和B,加上农家肥@ 10 tha - 1,显著提高了甘蔗和糖的产量,并维持了土壤肥力和甘蔗汁质量。
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引用次数: 0
Identification for Fertility in Multigerm Sugar Beet Germplasm Resources of Molecular Markers 多胚甜菜种质资源育性的分子标记鉴定
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-11 DOI: 10.1007/s12355-025-01617-y
Jinghao Li, Linlin Sun, Zhi Pi, Shengnan Li, Zedong Wu

A total of 116 multigerm sugar beet germplasm resources, which could be used as breeding material and lacked relevant fertility information, were evaluated for their fertility gene composition using different molecular marker techniques. Using variable number tandem repeats (VNTR)—specific primer TR1 identification of male-sterile cytoplasm (S) and normal cytoplasm (N) was conducted. The results showed that 26.76% of the germplasm resources were shown as N-cytoplasm; 24.13% of the germplasm resources were shown as S-cytoplasm; mixed cytoplasmic types accounted for 49.11%. Using cleaved amplified polymorphism sequences (CAPS)—specific primer S17 analysis of nucleus Rf1 loci fertility genes was performed; the results showed that 66 germplasm resources were identified as homozygous 1800 bp bands. Enzymatic digestion showed that 53 germplasm resources displayed pattern 5/5, and 13 germplasm resources showed pattern 4/5. Using DNA fragment length polymorphism (DFLP)—specific primer O7 analysis of nucleus Rf2 loci fertility genes was carried out; the results showed that 38 germplasm resources were identified as homozygous 1400 bp bands, and two germplasm resources were identified as homozygous 2600 bp bands. No sterile line or maintainer line was identified in this identification; the 116 multigerm sugar beet germplasm resources utilized in this experiment can serve as materials for subsequent breeding; initially, the fertility composition of these accessions remained unclear, hindering their effective utilization. Our research has systematically characterized their fertility profiles, enabling targeted selection of specific germplasm for hybrid combinations in the future breeding programs, aiming to provide a reference for the breeding of superior sugar beet varieties.

利用不同的分子标记技术,对116份可作为育种材料且缺乏相关育性信息的甜菜多胚种质资源进行育性基因组成评价。利用可变数串联重复序列(VNTR)特异性引物TR1对雄性不育细胞质(S)和正常细胞质(N)进行了鉴定。结果表明:26.76%的种质资源为n细胞质;24.13%的种质资源为s细胞质;混合细胞质类型占49.11%。利用裂解扩增多态性序列(CAPS)特异引物S17对细胞核Rf1位点的生育基因进行分析;结果表明,66份种质资源被鉴定为1800bp纯合子条带。酶切结果表明,53份种质资源表现出5/5模式,13份种质资源表现出4/5模式。利用DNA片段长度多态性(DFLP)特异性引物O7对细胞核Rf2位点的生育基因进行分析;结果表明,有38份种质资源被鉴定为1400bp纯合子带,2份种质资源被鉴定为2600bp纯合子带。本鉴定中未发现不育系或保持系;本试验利用的116个多胚甜菜种质资源可作为后续育种的材料;最初,这些土地的肥力组成仍然不清楚,妨碍了它们的有效利用。本研究系统地鉴定了它们的育性特征,为今后选育计划中有针对性地选择特定的种质组合提供依据,旨在为甜菜优良品种的选育提供参考。
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引用次数: 0
Post-emergence Weed Control in Sugarcane with Ready Mix of 2,4-D Sodium Salt 48% Plus Metribuzin 32% Plus Chlorimuron 0.8% WDG 2,4- d钠盐48% +美曲津32% +氯脲0.8% WDG对甘蔗苗期杂草的防治
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-11 DOI: 10.1007/s12355-025-01621-2
Simerjeet Kaur, Rajinder Pal, Tarundeep Kaur, Gurinder Singh, Manpreet Singh, M. S. Bhullar

Weed pressure at maximum vegetative growth stage in sugarcane has underscored the need to investigate post-emergence herbicides to decrease weed biomass and realize the productivity potential of sugarcane. This study assessed weed and productivity responses to different doses of three-way ready-mix post-emergence herbicide during spring 2017 and 2018 in Department of Agronomy at Punjab Agricultural University, Ludhiana, and spring 2019 and 2020 in PAU Regional Research Station, Kapurthala. The treatments were replicated thrice in randomized complete block design. Results indicated that 2020 g a.i. a.i. ha−1 of premix 2,4-D sodium salt 48% plus metribuzin 32% plus chlorimuron 0.8% WDG resulted in 136.2% and 133.9%, 87.9% and 375% and 41.9% and 37.9% reduction of biomass of monocots, dicots and sedges at Kapurthala and Ludhiana, respectively, compared to weedy plots at 60 DAA. Further, this herbicide resulted in 33.7% and 24.5% more millable canes and 38.6% and 35.2% higher cane yield at Kapurthala and Ludhiana, respectively, over unsprayed weedy check. It was concluded that post-emergence application of 2,4-D sodium salt 48% plus metribuzin 32% plus chlorimuron 0.8% WDG at 2020 g a.i. a.i. ha−1 effectively control the complex weed flora (monocots, dicots and sedges) and enhanced productivity of sugarcane in Indian sub-tropics.

甘蔗营养生长期的杂草压力表明,有必要研究出苗期除草剂,以减少杂草生物量,实现甘蔗的生产力潜力。本研究评估了旁遮普农业大学农学系2017年春季和2018年春季以及Kapurthala PAU区域研究站2019年春季和2020年春季对不同剂量的三种预混出苗后除草剂的杂草和生产力反应。在随机完全区组设计中重复三次治疗。结果表明:与杂草样地相比,在60 DAA条件下,2,4- d钠盐48% +杀虫灵32% +杀虫隆0.8% WDG的预混剂浓度为2020 g a.i. ha−1时,Kapurthala和Ludhiana单子房、双子房和禾草的生物量分别减少136.2%和133.9%、87.9%和375%、41.9%和37.9%。此外,该除草剂在卡普尔塔拉和卢迪亚纳的甘蔗产量分别比未施用除草剂的甘蔗产量高33.7%和24.5%,甘蔗产量分别提高38.6%和35.2%。结果表明,在2020g a.a.a.i. ha - 1条件下,苗期施用2,4- d钠盐48% +咪唑嗪32% +氯脲0.8% WDG,可有效防治印度亚热带地区复杂杂草区系(单子叶、双子叶和禾草),提高甘蔗生产力。
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引用次数: 0
Effects of Optimized Nitrogen Fertilizer Application on Chlorophyll Fluorescence Characteristics and Dry Matter Accumulation in Sugar Beet 优化施氮量对甜菜叶绿素荧光特性和干物质积累的影响
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-09 DOI: 10.1007/s12355-025-01620-3
Shi-Feng Dong, Lei Wei, Xing-Bo Tong, Yi-Na Zhang, Rong-Li Jia, Xu-Ming Xing, Shu-De Shi

Nitrogen plays crucial roles in photosynthesis and crop yields. In 2024, a field experiment was conducted with various nitrogen treatments (N0, N60, N120, N150, N180, N240, and N300) to evaluate the impact on photosystem activity, dry matter accumulation (DMA), and taproot development in sugar beet. The results show that nitrogen fertilization significantly reduced the fluorescence at the K-, J-, and I-steps, compared to controls, while enhancing absorption per active reaction center, trapped energy flux per active reaction center, and electron transport per active reaction center. In addition, maximum photochemical efficiency (φPo), photochemical energy conversion efficiency (φEo), and the probability of captured excitation energy being transferred to the electron transport chain (ψo) showed a single-peak pattern with increasing nitrogen, with a maximum value of ψo with the N180 treatment on July 22. During the growth stage, DMA and the rate thereof followed a single-peak trend, accompanied by increased root/shoot ratios and reduced source–sink activity. As nitrogen levels increased, DMA and the rate thereof increased during the early to mid-growth stages, while total and aboveground DMA rates peaked and subsequently declined during the sugar accumulation stage. Relative to N0, nitrogen application accelerated the rapid taproot DMA onset and the timing of the maximum accumulation rate, which is increased significantly. The N180 treatment resulted in the highest accumulation rate, with an increase of 43.48% relative to that without treatment. Increased nitrogen also increased the yields by 8.9–48.5%; the highest sugar yield, with the N180 treatment, surpassed those of other treatments by 5.55–40.94%. Optimal nitrogen application (N180) enhanced the PSII reaction center activity, improved the electron transport efficiency and reduced the non-photochemical energy dissipation. This increased the total DMA, optimized the source–sink balance, and effectively regulated the key parameters of taproot DMA, ultimately leading to increased sugar beet yields.

氮在光合作用和作物产量中起着至关重要的作用。为了研究不同氮肥处理(N0、N60、N120、N150、N180、N240和N300)对甜菜光系统活性、干物质积累(DMA)和主根发育的影响,于2024年进行了田间试验。结果表明,与对照相比,施氮显著降低了K-、J-和i -步的荧光,同时提高了每个活性反应中心的吸收、每个活性反应中心的捕获能通量和每个活性反应中心的电子输运。最大光化学效率(φPo)、光化学能转换效率(φEo)和捕获的激发能转移到电子传递链(ψo)的概率随氮的增加呈单峰模式,其中N180处理在7月22日达到最大值。在生长阶段,DMA及其速率呈单峰趋势,根系/梢比增加,源库活性降低。随着氮素水平的升高,生长前期和中期土壤直接需氧量和直接需氧量均呈上升趋势,而总需氧量和地上需氧量均在糖积累期达到峰值,随后下降。相对于氮肥,施氮加速了主根DMA的快速启动和最大积累速率的时间,显著增加。N180处理积累速率最高,比未处理提高43.48%。增施氮肥可使产量提高8.9 ~ 48.5%;以N180处理的糖产量最高,比其他处理高出5.55 ~ 40.94%。最佳施氮量(N180)提高了PSII反应中心活性,提高了电子传递效率,降低了非光化学能量耗散。这增加了总DMA,优化了源库平衡,有效调节了主根DMA的关键参数,最终提高了甜菜产量。
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引用次数: 0
Establishment and Methodology Validation of Latex-Enhanced Turbidimetric Immunoassay for Dextran Determination in Sugar 乳胶增强浊度免疫法测定糖中葡聚糖的建立及方法学验证
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-06-08 DOI: 10.1007/s12355-025-01612-3
Guowei Chang, Jun Liu, Bin Feng, Jun Chen, Qing Huang, Guiyun Liu, Xiuyu Luo, Zhide Li, Zengwei Huang, Dafeng Liang

Dextran, recognized as a deleterious component in sugar production, impairs the manufacturing process, thereby highlighting the need for an efficient detection method. The modified alcohol haze method, traditionally employed for dextran detection, is hindered by complex procedures and a lack of specificity. In contrast, immunonephelometry, a more contemporary method which detects antigen–antibody complexes via light scattering, offers the advantages of specificity, ease of operation, and rapid detection, yet it incurs higher cost due to the expensive production of monoclonal antibody. Addressing these limitations, we have developed a latex-enhanced turbidimetric immunoassay (LETIA) that significantly reduces assay costs while retaining the advantages of its predecessors. The antibody was purified cost-effectively using a hydroxyapatite column. After optimization, the preparation of immune latex showed high repeatability, with coefficients of variation (CV) between 2.12 and 3.58%. The method exhibited a robust linear response within the concentration range of 0–100 mg/L, as evidenced by an R2 value of 0.9985. The limit of detection (LOD) was 35 mg/kg sugar, which indicated an excellent sensitivity. The CV was between 1.51 and 3.14% in high- and low-level repeatability test. The recovery is between 93.8 and 105.8% in high- and low-level fortification experiments. Common interferents did not exhibit any significant interference. The antibody dosage and test cost were 0.00455 mg/test and 0.41 $/test, respectively, equating to a mere 1.5% and 4.8% of the immunonephelometry requirements, representing a significant reduction.

右旋糖酐在制糖过程中被认为是一种有害成分,它会影响制糖过程,因此需要一种有效的检测方法。传统上用于右旋糖酐检测的改进的醇雾法,由于程序复杂和缺乏特异性而受到阻碍。相比之下,免疫比浊法是一种更现代的通过光散射检测抗原-抗体复合物的方法,具有特异性、操作方便、检测快速的优点,但由于单克隆抗体的生产成本较高,因此成本较高。针对这些限制,我们开发了一种乳胶增强浊度免疫测定(LETIA),在保留其前身优势的同时显着降低了分析成本。该抗体采用羟基磷灰石柱纯化,成本效益高。优化后制备的免疫胶乳具有较高的重复性,变异系数(CV)在2.12 ~ 3.58%之间。该方法在0 ~ 100 mg/L的浓度范围内具有良好的线性响应,R2值为0.9985。检出限为35 mg/kg糖,具有良好的灵敏度。高低重复性检验的CV在1.51 ~ 3.14%之间。高低强化试验回收率在93.8% ~ 105.8%之间。普通干扰不表现出明显的干扰。抗体剂量和检测费用分别为0.00455 mg/次和0.41美元/次,仅相当于免疫比浊法要求的1.5%和4.8%,显着降低。
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引用次数: 0
Integrated Pest Management of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) on Sugar Beet 甜菜夜蛾(鳞翅目:夜蛾科)害虫综合治理
IF 2 3区 农林科学 Q2 AGRONOMY Pub Date : 2025-05-31 DOI: 10.1007/s12355-025-01614-1
Mohamed El Aalaoui, Mohamed Sbaghi

The management of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) on sugar beet is a significant challenge for growers. This study evaluates the efficacy of different treatments over two seasons (2022–2023 and 2023–2024) using a randomized complete block design. Ten treatments were evaluated: Spinosad (200 mL hL−1) (SD), Nicotiana glauca extract (10%) (NG), Ricinus communis extract (10%) (RC), the fungus Alternaria destruens (1 × 10⁸ conidia mL⁻1) (AD), and their combinations. Treatments were applied seven times per season, starting one month after planting, with subsequent applications at four-week intervals. Key variables assessed included leaf damage, larval density, plant canopy area, and root yield. Combined treatments, especially SD + NG + AD treatment, provided the most effective control. By the fourth month after the first treatment (4 MAFT), the SD + NG + AD treatment reduced leaf damage to 3.8% (2022–2023) and 4.2% (2023–2024), with larval densities dropping to 0.7 and 0.6 larvae/plant, respectively. Combined treatments enhanced plant growth, with SD + NG + AD treatment resulting in the largest plant canopy area (304.2 cm2 in 2022–2023 and 291.9 cm2 in 2023–2024). Root yield was significantly higher for combined treatments, with SD + NG + AD yielding 74.9 t/ha in 2022–2023 and 74.4 t/ha in 2023–2024. The untreated control exhibited the lowest efficacy. The treatment SD + NG + AD proved to be the most effective in reducing pest populations, improving plant growth, and enhancing root yield, providing a promising strategy for integrated pest management.

甜菜夜蛾(鳞翅目:夜蛾科)的防治是甜菜种植者面临的一个重大挑战。本研究采用随机完全区组设计,评估了两个季节(2022-2023和2023-2024)不同治疗的疗效。对10种处理进行了评价:Spinosad (200 mL hL−1)(SD)、Nicotiana glauca提取物(10%)(NG)、Ricinus communis提取物(10%)(RC)、Alternaria destruens (1 × 10⁸conidia mL毒枭)(AD)及其组合。每个季节施用七次,从播种后一个月开始,随后每四周施用一次。评估的关键变量包括叶片损害、幼虫密度、植物冠层面积和根系产量。综合处理,尤其是SD + NG + AD处理,控制效果最好。在第一次处理(4 MAFT)后第4个月,SD + NG + AD处理的叶片危害分别降低3.8%(2022-2023)和4.2%(2023-2024),幼虫密度分别降至0.7和0.6只/株。组合处理促进了植物生长,其中SD + NG + AD处理的植物冠层面积最大(2022-2023年为304.2 cm2, 2023-2024年为291.9 cm2)。组合处理根系产量显著提高,SD + NG + AD在2022-2023年产量为74.9 t/ha,在2023-2024年产量为74.4 t/ha。未处理的对照组疗效最低。结果表明,SD + NG + AD处理在减少害虫种群、促进植株生长和提高根系产量方面效果最好,为害虫综合治理提供了一种有前景的策略。
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引用次数: 0
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Sugar Tech
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