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Global Distribution, Identification, Pathogenesis, and Advanced Management Strategies for Sclerotium Root Rot in Sugar beet 甜菜硬根腐病的全球分布、鉴定、致病机理和先进管理策略
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-23 DOI: 10.1007/s12355-024-01478-x
Varucha Misra, A. K. Mall

Sclerotium root rot, caused by the fungus Sclerotium rolfsii, presents a significant challenge to sugar beet cultivation, particularly in tropical and warmer climates where high temperatures favor pathogen proliferation. This disease is prevalent in southern regions globally, where optimal conditions enable the fungus to produce white cottony mycelium and sclerotia rapidly. These sclerotia can be dispersed by wind or during agricultural activities, integrating into the soil profile and complicating disease management. The pathogen’s complex life cycle and broad host range exacerbate management difficulties. Early identification of symptoms is crucial for effective management, emphasizing the need for advanced diagnostic techniques. This study highlights recent advancements in managing Sclerotium root rot, focusing on biotechnological innovations and precision agriculture methods. Techniques such as CRISPR/Cas gene editing, artificial intelligence, satellite farming, and augmented reality offer promising solutions for disease control. CRISPR/Cas technology provides precise genetic modifications to enhance disease resistance in sugar beets. Artificial intelligence and satellite farming enable real-time monitoring and predictive analytics for early detection and management of the disease. Augmented reality tools facilitate farmer education and decision-making through immersive and interactive platforms. The integration of these advanced technologies presents a comprehensive approach to combating Sclerotium root rot, ensuring sustainable sugar beet production in affected regions. This study underscores the importance of leveraging cutting-edge innovations to address the complexities of pathogen management in agriculture.

由真菌 Sclerotium rolfsii 引起的硬根腐病给甜菜种植带来了巨大挑战,尤其是在热带和气候温暖的地区,因为那里的高温有利于病原体的扩散。这种病害流行于全球南部地区,那里的最佳条件使真菌能够迅速产生白色棉状菌丝和硬菌丝。这些硬孢菌丝可随风或在农业活动中散播,融入土壤剖面,使病害管理复杂化。病原体的生命周期复杂,寄主范围广泛,这加剧了管理难度。早期识别症状对有效管理至关重要,这就强调了对先进诊断技术的需求。本研究重点介绍了管理硬根腐病的最新进展,重点是生物技术创新和精准农业方法。CRISPR/Cas 基因编辑、人工智能、卫星农业和增强现实等技术为疾病控制提供了前景广阔的解决方案。CRISPR/Cas 技术可提供精确的基因修饰,增强甜菜的抗病性。人工智能和卫星农业可进行实时监测和预测分析,以便及早发现和管理病害。增强现实工具通过身临其境的互动平台促进农民教育和决策。这些先进技术的集成提供了一种防治硬根病的综合方法,可确保受影响地区甜菜的可持续生产。这项研究强调了利用尖端创新技术解决农业病原体管理复杂问题的重要性。
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引用次数: 0
The Leaching Potential and Recovery of 15-N-Fertilizer by Sugarcane Cultivated in Sandy Soil 沙质土壤中甘蔗对 15-N 肥料的浸出潜力和回收率
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-22 DOI: 10.1007/s12355-024-01479-w
Ariane Márcia de Sousa Silva, Emídio Cantídio Almeida de Oliveira, Joel José de Andrade, Amanda Michele Santos Lima, Brivaldo Gomes de Almeida, Paulo Cesar Ocheuze Trivelin, Fernando José Freire, Dante Hugo Guimarães

Leaching is an important loss pathway that limits N-fertilizer recovery by sugarcane. Our study evaluated the potential for leaching and recovery of 15N-fertilizer by sugarcane cultivated in sandy soil. Three nitrogen fertilization management strategies were tested: single dose (80 kg ha−1), split (40 kg ha−1 + 40 kg ha−1) and supplementation (80 kg ha−1 + 40 kg ha−1), one more control; without nitrogen (N). Ammonium nitrate (AN) and ammonium sulphate (AS) were the sources N used. Leaching was determined by the N-mineral concentration (NH4+, NO2 and NO3) and 15N-fertilizer in the soil solution at a depth of 0.7 m. Nitrogen recovery was quantified using 15N-fertilizer in the sugarcane shoots. Significant leaching occurred in the first 60 days after fertilization (DAF), but splitting the fertilization reduced N-mineral leaching by 50%. During this period, 78% of the nitrogen in the leachate originated from the fertilizer (NDFF(L)). The contribution of nitrogen fertilization was highest in the first 30 DAF, where the fertilizer accounted for 39% of the nitrogen absorbed by sugarcane. But the plant’s nitrogen recovery (R) was limited to only 4% of the applied nitrogen. At harvest, the R of 15N-fertilizer by sugarcane was less than 20%. Splitting or applying a single dose of fertilizer did not improve nitrogen recovery at the end of the cycle. Our study showed that the leaching and recovery of 15N-fertilizer were found to be proportional to the applied doses according to the management strategies and nitrogen sources compensated for nitrogen leaching throughout the cycle.

淋溶是限制甘蔗氮肥回收的一个重要损失途径。我们的研究评估了在沙质土壤中种植的甘蔗对 15N 肥料的浸出和回收潜力。我们测试了三种氮肥管理策略:单次施肥(80 千克/公顷-1)、分次施肥(40 千克/公顷-1 + 40 千克/公顷-1)和补充施肥(80 千克/公顷-1 + 40 千克/公顷-1)。使用的氮源为硝酸铵(AN)和硫酸铵(AS)。根据 0.7 米深土壤溶液中的氮矿物质浓度(NH4+、NO2- 和 NO3-)和 15N 肥料确定浸出情况。在施肥后的前 60 天(DAF),出现了明显的沥滤现象,但分次施肥使氮矿物沥滤减少了 50%。在此期间,浸出液中 78% 的氮来自肥料(NDFF(L))。在前 30 DAF 中,氮肥的作用最大,占甘蔗吸收氮量的 39%。但甘蔗的氮回收率(R)仅为施氮量的 4%。收获时,甘蔗对 15N 肥料的氮回收率不到 20%。分次施肥或单次施肥并不能提高周期结束时的氮素回收率。我们的研究表明,根据管理策略,15N 肥料的沥滤和回收与施用剂量成正比,氮源在整个周期内都能补偿氮的沥滤。
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引用次数: 0
Simulating Sugarcane Elongation by a Multi-Parameter Generalized Logistic Model with Meteorological Effect 用带气象效应的多参数广义 Logistic 模型模拟甘蔗伸长率
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-21 DOI: 10.1007/s12355-024-01477-y
Guojun Zheng, Pengcheng Ma, Qinlong Wang, Hairong Huang, Ting Luo, Xiang Li, Meixin Yan, Zeping Wang, Guanghu Zhu

Sugarcane is a raw material used to produce sugar and ethanol. Its growth is influenced by meteorological factors. This study aimed at providing a modeling framework to simulate sugarcane stalk elongation by considering the planting stage and meteorological factors. Using plant height data and meteorological data collected in Guangxi (China) during 2018–2022, combined with the cumulative temperature function, a multi-parameter generalized logistic model was constructed to simulate changes in plant height for spring planting and ratoon sugarcane. The meteorological effects on sugarcane growth were further quantified. Expressions of six models simulating sugarcane growth were given by fitting the data. The results showed that the selected model could simulate the elongation period of sugarcane well, and it simulated Guitang No. 42 sugarcane most effectively. Sensitivity analysis showed that effective cumulative temperature and cumulative precipitation had the greatest influence on sugarcane growth and growing duration. It was also found that increasing rainfall to the optimum level in August was the most favorable for sugarcane growth.

甘蔗是一种用于生产糖和乙醇的原料。其生长受到气象因素的影响。本研究旨在通过考虑种植阶段和气象因素,提供一个模拟甘蔗茎秆伸长的建模框架。利用在广西(中国)收集到的2018-2022年植株高度数据和气象数据,结合积温函数,构建了一个多参数广义Logistic模型,模拟春植甘蔗和轮作甘蔗的植株高度变化。进一步量化了气象对甘蔗生长的影响。通过数据拟合,给出了模拟甘蔗生长的六个模型的表达式。结果表明,所选模型能很好地模拟甘蔗的伸长期,其中对桂糖 42 号甘蔗的模拟效果最好。敏感性分析表明,有效积温和累积降水对甘蔗生长和生长期的影响最大。研究还发现,将 8 月份的降雨量增加到最佳水平最有利于甘蔗生长。
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引用次数: 0
Trash Amended with Trichoderma Effects on Cane Yield, Soil Carbon Dynamics, and Enzymatic Activities under Plant–Ratoon System of Sugarcane in Calcareous Soil 用毛霉菌改良垃圾对石灰性土壤中甘蔗种植-轮作系统下甘蔗产量、土壤碳动态和酶活性的影响
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-18 DOI: 10.1007/s12355-024-01467-0
Navnit Kumar, Sumit Sow, Lalita Rana, Shivani Ranjan, A. K. Singh

Soil organic carbon (SOC) management is crucial for promoting crop growth and maintaining sustainability. In calcareous soils, there is a consistent decline in SOC in agricultural soils, with many soils exhibiting notably low SOC content. In this context, evaluating the potential of sugarcane–ratoon systems should be a priority, as these trashes play a vital role in sustaining SOC levels, enhancing soil health, and ultimately influencing cane yield positively. We investigated the effects of trash management and the application of Trichoderma on the growth, yield, SOC, and its fractions in a sugarcane plant–ratoon system. Thus, a field experiment was conducted in a randomized block design with five replications with four treatments, which consisted trash mulching (RM) or incorporation (RI), or removal (RR) with or without Trichoderma (T). RI + T influenced the growth and yield attributes viz. plant height, tillers, cane diameter, and millable canes positively. An improvement of 12.0 and 21.3% in cane yield was recorded after the harvest of the third ratoon crop as compared to RM and RR, respectively. The highest SOC stock (28.84 Mg ha−1) was recorded with RI + T treatment. Furthermore, RI + T plots had 35.9% higher total organic carbon (TOC) with 31.9, 31.8, 32.0, and 32.1% higher very labile, labile, less labile, and non-labile C fractions, respectively, than RR plots. Moreover, trash incorporation with Trichoderma inoculation treatment improved soil respiration, soil microbial biomass carbon, soil microbial biomass N, and soil protein by 38.4, 20.1, 45.2, and 31.1%, respectively, as compared to trash mulching without Trichoderma. Similarly, RI + T treatment recorded higher C sequestration rate (0.89 Mg ha−1 year−1) and C sequestered (3.56 Mg ha−1). Significantly higher dehydrogenase activity (19.8%) and alkaline phosphatase activity (28.0%) were observed in RI + T treatment as compared to RM treatment. Thus, trash incorporation with Trichoderma is recommended for higher soil C sequestration and soil health for sustained sugarcane–ratoon productivity.

土壤有机碳(SOC)管理对于促进作物生长和保持可持续性至关重要。在石灰性土壤中,农业土壤中的 SOC 持续下降,许多土壤的 SOC 含量明显偏低。在这种情况下,评估甘蔗-轮作系统的潜力应该是一个优先事项,因为这些垃圾在维持 SOC 含量、增强土壤健康以及最终对甘蔗产量产生积极影响方面发挥着至关重要的作用。我们研究了垃圾管理和毛霉的应用对甘蔗植物-轮作系统的生长、产量、SOC 及其组分的影响。因此,我们进行了一项田间试验,采用随机区组设计,五次重复,四种处理,包括垃圾覆盖(RM)或纳入(RI),或清除(RR)加或不加毛霉菌(T)。RI + T 对植株高度、分蘖、甘蔗直径和可磨茎等生长和产量属性有积极影响。与 RM 和 RR 相比,第三轮作物收获后甘蔗产量分别提高了 12.0% 和 21.3%。RI + T 处理的 SOC 储量最高(28.84 兆克/公顷-1)。此外,与 RR 地块相比,RI + T 地块的总有机碳(TOC)高出 35.9%,其中极易变、易变、较易变和非易变 C 部分分别高出 31.9、31.8、32.0 和 32.1%。此外,与未接种毛霉的垃圾覆膜相比,接种毛霉的垃圾覆膜土壤呼吸作用、土壤微生物生物量碳、土壤微生物生物量氮和土壤蛋白质分别提高了 38.4%、20.1%、45.2% 和 31.1%。同样,RI + T 处理的固碳率(0.89 兆克/公顷-1 年-1)和固碳量(3.56 兆克/公顷-1)也更高。与 RM 处理相比,RI + T 处理的脱氢酶活性(19.8%)和碱性磷酸酶活性(28.0%)明显更高。因此,建议在垃圾中加入毛霉菌,以提高土壤固碳能力和土壤健康水平,从而保持甘蔗-芸苔素的持续生产力。
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引用次数: 0
Optimization of Processing Techniques for Sugarcane Juice Self Life Expansion: pH Adjustment and Pasteurization Strategies 优化加工技术以延长甘蔗汁自身寿命:pH 值调整和巴氏杀菌策略
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-17 DOI: 10.1007/s12355-024-01438-5
Priyanka Chauhan, Manisha Kaushal, Devina Vaidya, Anil Gupta, Faruk Ansari, Shreya Kashyap, Vinaykumar Rachappanavar

Sugarcane juice (SJ), known for its rich nutrients, faces challenges in processing and marketing due to rapid spoilage by microbes and enzymes. This study aimed to develop techniques to extend the shelf life of SJ. We investigated optimizing SJ’s pH to 4.00 using different acids. Based on sensory tests, orthophosphoric acid (OPA) was chosen as the most acceptable acidulant. The pH-adjusted juice was then heated at various temperatures (50–90 °C). Heating at 90 °C significantly reduced a spoilage enzyme (PPO activity) compared to untreated juice. Pasteurization time at 90 °C was further optimized to 15 min based on PPO activity and microbial control. The final preservation method involved preheating pH-adjusted juice (with OPA) to 60 °C, followed by hot filling into pre-sterilized bottles and pasteurization at 90 °C for 15 min. This method ensured juice quality and storage stability. Storage studies showed that the pH-adjusted and pasteurized SJ maintained good quality for three months under various storage conditions. This study demonstrates the potential of this technology for efficient SJ storage and utilization, opening doors for commercial applications.

甘蔗汁(SJ)以其丰富的营养而闻名,但由于微生物和酶的快速腐败作用,它在加工和销售方面面临着挑战。本研究旨在开发延长甘蔗汁保质期的技术。我们研究了使用不同的酸将 SJ 的 pH 值优化至 4.00。根据感官测试,正磷酸(OPA)被选为最可接受的酸化剂。然后在不同的温度(50-90 °C)下加热经过 pH 调整的果汁。与未经处理的果汁相比,90 °C加热能明显降低一种腐败酶(PPO活性)。根据 PPO 活性和微生物控制情况,90 °C 巴氏杀菌时间进一步优化为 15 分钟。最终的保存方法是将 pH 值调整后的果汁(含 OPA)预热至 60 °C,然后热灌装到预先灭菌的瓶中,并在 90 °C 下巴氏灭菌 15 分钟。这种方法确保了果汁的质量和储存稳定性。贮藏研究表明,在各种贮藏条件下,经过 pH 值调整和巴氏杀菌的 SJ 在三个月内都能保持良好的品质。这项研究证明了该技术在高效储存和利用 SJ 方面的潜力,为商业应用打开了大门。
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引用次数: 0
Field Evaluation of Sugarcane Genotypes for Resistance to Leaf Scald Disease in China 中国甘蔗基因型对叶片灼伤病抗性的田间评估
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-16 DOI: 10.1007/s12355-024-01476-z
Yisha Li, Jingxia Du, Meilin Li, Wei Yao, Muqing Zhang

Leaf scald is one of the major bacterial diseases causing severe losses to the sugarcane industry. Utilizing disease-resistant genotypes has proved to be the safest, most economical, and effective method to control it. Therefore, we evaluated leaf scald disease (LSD) on 166 sugarcane genotypes in a field trial over three years (2019–2021). We found that the disease incidence and severity varied among genotypes, and the percentage of diseased genotypes differed significantly across crop seasons. ANOVA analysis revealed that Genotype, Year, and Genotype-by-Year interaction significantly affected LSD occurrence. However, the broad-sense heritability (HB2) for disease incidence (IC) and severity (DI) over three years was 0.72 and 0.68, respectively, suggesting that genetic factors play an essential role. To better classify the level of disease resistance, we performed cluster analysis using three years of disease incidence and severity; the accuracy of the cluster analysis was verified by discriminant analysis. Of the 166 sugarcane genotypes, 35 (21.08%) were highly resistant, 67 (40.36%) resistant, 24 (14.46%) moderately susceptible, 24 (14.46%) susceptible, and 16 (9.64%) highly susceptible. The total probability of discriminant analysis was 90.36%, indicating that the classification results were relatively accurate. The outcomes of this study provide a scientific basis for breeding and selecting resistant genotypes for commercial cultivation.

叶烫病是给甘蔗产业造成严重损失的主要细菌性病害之一。事实证明,利用抗病基因型是最安全、最经济、最有效的防治方法。因此,我们在三年(2019-2021 年)的田间试验中对 166 种甘蔗基因型的叶烫病(LSD)进行了评估。我们发现,不同基因型的病害发生率和严重程度各不相同,不同作物季节患病基因型的比例也有显著差异。方差分析显示,基因型、年份和基因型与年份的交互作用对 LSD 的发生有显著影响。然而,三年中病害发生率(IC)和严重程度(DI)的广义遗传率(HB2)分别为 0.72 和 0.68,表明遗传因素起着至关重要的作用。为了更好地划分抗病水平,我们利用三年的发病率和严重程度进行了聚类分析;聚类分析的准确性通过判别分析得到了验证。在 166 个甘蔗基因型中,35 个(21.08%)高度抗病,67 个(40.36%)抗病,24 个(14.46%)中度感病,24 个(14.46%)感病,16 个(9.64%)高度感病。判别分析的总概率为 90.36%,表明分类结果相对准确。研究结果为商业化种植中抗性基因型的培育和选育提供了科学依据。
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引用次数: 0
Economical Sugar Beet Production: Biotechnological Advances to Improve Yield in Conditions of Abiotic and Biotic Stress 经济型甜菜生产:在非生物和生物压力条件下提高产量的生物技术进步
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-16 DOI: 10.1007/s12355-024-01461-6
Radmila Bojović, Vera Popović, Dragana Popović, Radivoj Prodanović, Ružica Đukić, Jelena Bošković, Mihajlo Ćirić, Vladimir Filipović

Sugar beet—Beta vulgaris L. is the second largest sugar-producing crops, after sugarcane, accounting around 40% of total global sugar output in world. The largest areas are in Europe. It is grown for sugar and many different products. In recent times, more and more people are talking about the importance of sugar beet, as a fodder plant, but also as a plant for biofuel production. Sugar beet yield and quality are determined by genotype and environment. In this study, the state and analysis of the production of the sugar beet crop and influence of factors during the crop growth is studied. The main factor influencing the crop yield and bioethanol yield is the weather variability, then climate change. Irrigation in semi-arid countries, fertilizer and crop management have great importance on the yield. The objective of the current study was thus to analyze the components productivity of sugar beet, in the world and in Serbia, and to show the influence of meteorological conditions and of cultivation technology on the success of production. World sugar production in the 2021/2022 market year had a surplus of 10 million tons and was higher by 3%, due to increased production in India, the EU and Thailand. Higher consumption of this culture is expected in the coming period. Biggest drivers of this growth are India, China, the Philippines and the European Union. In Serbia, in 2022 sugar beet was sown on 39,411 ha. Compared to the ten-year average, this is 26% less. The average yield of sugar beet in the world for the five-year period amounted to 59,151 ha and varied from 60,766 ha, in 2022, to 57,104 ha, in 2018. In Serbia the average yield was 51,285 t/ha, and varied from 48,005 t/ha in 2022, to 54,193 t/ha in 2019. In Serbia, increasing of areas under sugar beet and increasing of yield is predicted. It can be concluded that sugar beet is a very important farming plant both in the world and in Serbia.

甜菜是仅次于甘蔗的第二大产糖作物,约占全球糖总产量的 40%。最大的种植区在欧洲。种植甜菜是为了制糖和生产多种产品。近来,越来越多的人开始谈论甜菜的重要性,它不仅是一种饲料作物,也是一种生产生物燃料的植物。甜菜的产量和质量由基因型和环境决定。本研究对甜菜作物的生产状况和分析以及作物生长过程中各种因素的影响进行了研究。影响作物产量和生物乙醇产量的主要因素是天气变化,其次是气候变化。半干旱国家的灌溉、肥料和作物管理对产量有重要影响。因此,本研究的目的是分析世界和塞尔维亚甜菜的各部分产量,并说明气象条件和种植技术对成功生产的影响。由于印度、欧盟和泰国的增产,2021/2022 市场年度世界糖产量过剩 1000 万吨,增长 3%。预计在未来一段时间内,这种文化的消费量将会增加。印度、中国、菲律宾和欧盟是这一增长的主要驱动力。在塞尔维亚,2022 年甜菜播种面积为 39,411 公顷。与十年平均值相比,减少了 26%。世界甜菜的五年平均产量为 59 151 公顷,从 2022 年的 60 766 公顷到 2018 年的 57 104 公顷不等。塞尔维亚的平均产量为 51 285 吨/公顷,从 2022 年的 48 005 吨/公顷到 2019 年的 54 193 吨/公顷不等。预计塞尔维亚甜菜种植面积将增加,产量将提高。因此,甜菜在世界和塞尔维亚都是非常重要的农作物。
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引用次数: 0
Evaluation of Saccharum and Erianthus Introgressed Early Generation Novel Sugarcane Hybrids as Potential Sources of Biomass for Cogeneration 作为热电联产生物质潜在来源的 Saccharum 和 Erianthus 早代新甘蔗杂交种的评估
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-12 DOI: 10.1007/s12355-024-01421-0
Sreenivasa Venkatarayappa, Mahadevaiah Channappa, Amaresh, Ravinder Kumar, Arun Kumar Raja, Mintu Ram Meena, Anna Durai Ayyadurai, Appunu Chinnaswamy, Hemaprabha Govindakurup, Govindaraj Perumal

Sugarcane is an important industrial crop and an excellent feedstock for bio-energy as an alternate to fossil energy source. Early generation hybrids were developed from the introgression of commercial varieties with wild grass (Saccharum spontaneum) and wild relative (Erianthus spp.) and the resultant hybrids produced higher cane fibre and harvestable biomass than commercial varieties under cultivation. The present study consists of 57 early generation hybrids (31 BMC and 26 BM) derived from interspecific and intergeneric hybridization of commercial sugarcane varieties with Saccharum complex (Saccharum officinarum, S. spontaneum, S. robustum, S. barberi and S. sinense) and Erianthus arundinaceus evaluated in randomized block design (RBD) trials over two consecutive seasons, and significant differences were observed for juice quality, cane parameters, fibre and biomass traits. Significant positive correlation was observed for fresh and dry biomass yield with number of shoots at 120 days and number of millable canes at 360 days. Fibre per cent had shown highly significant positive correlation with dry biomass per cent and dry biomass yield (t/ha). The PCA analysis has revealed that PC1 and PC2 were responsible for 70.33% and 67.99% of variance, respectively, while PC2 and PC3 were responsible for 41.25% and 46.96% of the variance, respectively. Cluster analysis revealed that four hybrids (BMC 6, BMC 17, BMC 25, and BMC 27) of BMC group and six hybrids (BM 5, BM 6, BM 11, BM 12, BM 14, and BM 16) of BM groups were numerically superior for fresh biomass yield (> 240 t/ha) and higher fibre per cent (> 20%) than the energy cane standards SBIEC 11001 and SBIEC 11003, respectively. These clones need further validation before could be exploited for cogeneration and as a parents for development of energy canes through hybridization.

甘蔗是一种重要的工业作物,也是替代化石能源的生物能源的极佳原料。早期杂交种是由商业品种与野生禾本科植物(Saccharum spontaneum)和野生近缘植物(Erianthus spp.)杂交培育而成的。本研究包括 57 个早期杂交种(31 个 BMC 和 26 个 BM),这些杂交种是由商业甘蔗品种与 Saccharum complex(Saccharum officinarum、S. spontaneum、S. robustum、S. barberi 和 S. sinense)和 Erianthus arundinaceus 在随机区组设计(RBD)试验中进行种间杂交和属间杂交产生的,在连续两季的试验中进行了评估,观察到在果汁质量、甘蔗参数、纤维和生物量性状方面存在显著差异。新鲜和干生物量产量与 120 天时的芽数和 360 天时的可磨蔗数呈显著正相关。纤维百分比与干生物量百分比和干生物量产量(吨/公顷)呈极显著的正相关。PCA 分析显示,PC1 和 PC2 分别占变异的 70.33% 和 67.99%,而 PC2 和 PC3 分别占变异的 41.25% 和 46.96%。聚类分析显示,BMC 组的 4 个杂交种(BMC 6、BMC 17、BMC 25 和 BMC 27)和 BM 组的 6 个杂交种(BM 5、BM 6、BM 11、BM 12、BM 14 和 BM 16)的新鲜生物量产量(> 240 吨/公顷)和纤维率(> 20%)在数量上分别优于能源甘蔗标准 SBIEC 11001 和 SBIEC 11003。这些克隆需要进一步验证,才能用于热电联产,并通过杂交作为开发能源甘蔗的亲本。
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引用次数: 0
Genetic Diversity Analysis of Red Beet Germplasm Resources Using CEAP Molecular Markers 利用 CEAP 分子标记分析红甜菜种质资源的遗传多样性
IF 1.8 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-09 DOI: 10.1007/s12355-024-01473-2
Zining Wang, Xiangjia Wu, Zhi Pi, Zedong Wu, Shengnan Li

Red beet is a significant crop, particularly in sugar production. This study aimed to develop a molecular identification system and cis-element amplified polymorphism (CEAP) fingerprints for red beet to address the issues related to the proliferation of counterfeit seeds and to enhance the understanding of the genetic relationships among red beet germplasm resources. By developing these tools, this study aimed to facilitate the identification of distinct red beet germplasm resources, ensure product integrity, and safeguard the intellectual property rights of new varieties. This study amplified 32 red beet varieties (lines) using polymerase chain reaction with 17 CEAP primers. The amplified products were then analyzed using 2% agarose gel electrophoresis. The electrophoretic test results indicated that the mean values of genetic diversity indices such as Na, Ne, I, He, Nm, and polymorphism information content for each primer were 6.5882, 3.8766, 1.4836, 0.7088, 0.2532, and 0.6649, respectively. The genetic distances among the varieties (lines) ranged between 0.129 and 0.404. The UPGMA cluster analysis revealed that the 32 red beet varieties (lines) could be divided into six groups. The fingerprints of the red beet varieties (lines) were generated using three key primers: GATAA6, TGAC28, and AAAG25. The findings of this study provided a theoretical basis for identifying, protecting, and tracing different red beet varieties, as well as advancing innovation in germplasm and associated intellectual property rights.

红甜菜是一种重要的作物,尤其是在制糖业中。本研究旨在开发红甜菜分子鉴定系统和顺式元素扩增多态性(CEAP)指纹图谱,以解决假冒种子泛滥的相关问题,并加深对红甜菜种质资源之间遗传关系的了解。通过开发这些工具,本研究旨在促进对不同红甜菜种质资源的鉴定,确保产品的完整性,并保护新品种的知识产权。本研究使用 17 种 CEAP 引物进行聚合酶链式反应,扩增了 32 个红甜菜品种(品系)。然后使用 2% 琼脂糖凝胶电泳对扩增产物进行分析。电泳检测结果表明,各引物的遗传多样性指数(如 Na、Ne、I、He、Nm 和多态性信息含量)的平均值分别为 6.5882、3.8766、1.4836、0.7088、0.2532 和 0.6649。品种(品系)间的遗传距离介于 0.129 和 0.404 之间。UPGMA 聚类分析显示,32 个红甜菜品种(系)可分为 6 组。红甜菜品种(品系)的指纹图谱由三个关键引物产生:GATAA6、TGAC28 和 AAAG25。这项研究的结果为鉴定、保护和追踪不同的红甜菜品种以及推动种质创新和相关知识产权提供了理论依据。
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引用次数: 0
Evaluation of Fulgoraecia melanoleuca (Fletcher) (Lepidoptera: Epipyropidae) Releases for the Management of Pyrilla perpusilla (Walker) (Hemiptera: Lophopidae) on Sugarcane in Punjab, India 在印度旁遮普邦评估释放的 Fulgoraecia melanoleuca (Fletcher) (鳞翅目:Epipyropidae)对甘蔗上的 Pyrilla perpusilla (Walker) (半翅目:Lophopidae)的防治效果
IF 1.9 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-08-07 DOI: 10.1007/s12355-024-01474-1
Neethu Maria Augustine, P. S. Shera, Rajinder Kumar, Sudhendu Sharma

Fulgoraecia melanoleuca (Fletcher) is an important ecto-parasitoid of Pyrilla perpusilla (Walker) nymphs and adults. The study aimed to standardize the augmentative releases of F. melanoleuca life stages (eggs and cocoons) for eco-friendly management of P. perpusilla in sugarcane crop. The releases of F. melanoleuca cocoons (2000, 4000, and 6000 cocoons ha−1) and eggs (0.2, 0.4, and 0.6 million eggs ha−1) were compared with chemical control (chlorpyriphos 20 EC @1500 ml ha−1) and untreated control. Two releases were carried out at monthly interval during 1st week of August and 1st week of September. The pyrilla population decreased progressively with increase in released cocoons (2000, 4000, and 6000 ha−1) and eggs (0.2, 0.4, and 0.6 million ha−1). The augmentative releases play a noteworthy role in increasing the parasitoid count (egg masses, cocoons and adults) in the released plots. Overall, the parasitism (nymph + adult) increased with increase in doses and it was significantly higher when parasitoids were released at higher doses, i.e. 0.6 million eggs ha−1 (52.80%) and 6000 cocoons ha−1 (50.60%). However, chlorpyriphos 20 EC (1500 ml ha−1) was found to be highly detrimental to the parasitoid as no parasitism was observed in insecticide-sprayed plots. In conclusion, two releases of F. melanoleuca parasitoid (0.6 million eggs ha−1 or 6000 cocoons ha−1) at monthly interval (1st week of August and 1st week of September) proved better due to higher parasitism rate, increase in parasitoid population, and reduction in pyrilla population and also rendered higher cane yield and better juice quality. The findings will help in the eco-friendly management of P. perpusilla in sugarcane.

Fulgoraecia melanoleuca (Fletcher) 是 Pyrilla perpusilla (Walker) 若虫和成虫的重要外寄生虫。该研究旨在规范 F. melanoleuca 生命阶段(卵和茧)的增殖释放,以生态友好的方式管理甘蔗作物中的 P. perpusilla。将释放的黑翅玉蟾蜍茧(2000、4000 和 6000 个茧公顷-1)和卵(0.2、0.4 和 0.6 百万卵公顷-1)与化学防治(氯虫苯甲酰胺 20 EC @1500 毫升公顷-1)和未处理的对照进行了比较。每月分别在八月的第一周和九月的第一周进行两次释放。随着释放茧(2000、4000 和 6000 公顷-1)和卵(0.2、0.4 和 0.6 百万公顷-1)的增加,海百合的数量逐渐减少。增殖释放在增加释放地块的寄生虫数量(卵块、茧和成虫)方面发挥了显著作用。总体而言,寄生虫数量(若虫+成虫)随着剂量的增加而增加,当寄生虫释放剂量较高时,寄生虫数量显著增加,即 0.6 百万虫卵公顷-1(52.80%)和 6000 个茧公顷-1(50.60%)。然而,氯虫苯甲酰胺 20 EC(1500 毫升/公顷-1)对寄生虫非常有害,因为在喷洒杀虫剂的地块上没有观察到寄生现象。总之,在每月间隔期(8 月第一周和 9 月第一周)释放两次 F. melanoleuca 寄生虫(60 万个卵公顷-1 或 6000 个茧公顷-1)证明效果更好,因为寄生率更高,寄生虫数量增加,蚜虫数量减少,而且甘蔗产量更高,果汁质量更好。这些研究结果将有助于对甘蔗中的 P. perpusilla 进行生态友好型管理。
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引用次数: 0
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Sugar Tech
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