Analytical biochemistry最新文献_第7页

A novel sandwich hybridization assay method and probe sets for cyanobacterial harmful algal bloom detection 一种新的蓝细菌有害藻华检测夹心杂交检测方法及探针装置

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-10-29 DOI: 10.1016/j.ab.2025.116002

Katelyn M. Brown , Dianne I. Greenfield , George S. Bullerjahn

A novel, rapid (2.5 h) sandwich hybridization assay (SHA) method for the detection of harmful cyanobacterial genera has been developed based on oligonucleotide hybridization to genus-specific sequences within the 16S ribosomal RNA. Unlike previously-developed 96-well plate SHA formats, this modification does not involve a robotic processor to maneuver reagents across the plate. Rather, it requires manual pipetting each reagent sequentially into the same well and washing the 96-well plate between each addition, thereby increasing sample throughput. Another key difference is the colorimetric reporter molecule. Yet, it enables specific detection of Planktothrix, Raphidiopsis and the Anabaena-Dolichospermum-Aphanizomenon (ADA) clade of the Nostocales. As these are the most common planktonic bloom-forming cyanobacteria, the method can be used to assess potential threats posed by these taxa, specifically cyanotoxins that are associated with particular bloom-forming taxa. The assays detected target groups and standard curves were created from cultured cyanobacteria biomass. Here, the SHA method, calibration, and the field performance of the assay on cyanobacterial bloom biomass is described. Linear standard curves were established for each assay, with a Planktothrix and Raphidiopsis quantification range of 10–500 mm³ L⁻¹ 100 μL homogenate⁻¹ and a range of 50–500 mm³ L⁻¹ 100 μL homogenate⁻¹ for the ADA clade assay. In environmental samples from three Ohio waterbodies, the assays detected the target genus in a mixed cyanobacterial community. This new SHA protocol will be highly beneficial to both management and research communities because it considerably broadens the capability to rapidly detect multiple cyanobacterial genera that cause persistent toxic blooms.

建立了一种新的快速（2.5 h）夹心杂交法（SHA）检测有害蓝藻属的方法，该方法基于16S核糖体RNA中属特异性序列的寡核苷酸杂交。与以前开发的96孔板SHA格式不同，这种修改不涉及机器人处理器来操纵试剂穿过板。相反，它需要手动将每种试剂依次移液到同一孔中，并在每次添加之间清洗96孔板，从而提高样品吞吐量。另一个关键的区别是比色报告分子。然而，它可以特异性检测浮游thrix， Raphidiopsis和Nostocales的anabaena - dolichosperum - aphanizomena （ADA）分支。由于这些是最常见的浮游藻华形成蓝藻，该方法可用于评估这些分类群构成的潜在威胁，特别是与特定藻华形成分类群相关的蓝藻毒素。实验检测的目标群体和标准曲线是由培养的蓝藻生物量创建的。在这里，SHA方法，校准和现场性能的分析蓝藻华生物量描述。每项检测均建立线性标准曲线，其中浮游thrix和Raphidiopsis的定量范围为10-500 mm3 L−1 100 μL匀浆−1，ADA分支检测的定量范围为50-500 mm3 L−1 100 μL匀浆−1。在俄亥俄州三个水体的环境样本中，检测到混合蓝藻群落的目标属。这种新的SHA协议将对管理和研究界都非常有益，因为它大大扩大了快速检测导致持续有毒繁殖的多种蓝藻属的能力。

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引用次数: 0

Corrigendum to“Development of a green polymer-based sensor for enhanced iron detection in diverse biological, food and environmental matrices” [Analyt. Biochem. 705 (2025) 115927] “开发一种绿色聚合物传感器，用于增强对各种生物、食品和环境基质中的铁的检测”[分析员]的勘误。生物化学学报，2015(5):1145 - 1145。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-17 DOI: 10.1016/j.ab.2025.116012

Rania A. Hussien

引用次数: 0

One-step purification of a bioactive PAK1-derived peptide 生物活性pak1衍生肽的一步纯化

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.ab.2025.116014

Djamali Muhoza , Emily P. Esquivel , Stacy R. Hunter , Patience S. Okoto , Thallapuranam K.S. Kumar , Paul D. Adams

The serine/threonine kinase PAK1 serves as a mediator of cytoskeletal reorganization and cancer-related signaling downstream of the small GTPases. Due to the challenges in purifying PAK1 complexes, a 46-residue peptide from PAK1, is widely used to study PAK1-Cdc42 signaling. Traditionally, this purification involved multi-step chromatography of recombinant GST-PBD46 complexes, yielding approximately 1 mg per 1.5 L culture. In this study, a 30 min heat treatment step after thrombin cleavage was used to precipitate GST while leaving pure PBD46 in solution. This step eliminated the need for further affinity and size-exclusion chromatography steps. This improved protocol produces proteins with a 6.5-fold higher yield, halves the purification processing time, and produces peptides with ≥95 % purity. Mass spectrometry, CD, fluorescence, and ¹H–¹⁵N HSQC NMR confirmed the heat-purified PBD46's identity, structure, folding, and binding to Cdc42. The method also successfully separated other small peptides (e.g., ACK1) but not larger folded proteins. This rapid and scalable approach facilitates peptide production and biochemical studies without compromising the structural or functional integrity. We believe that the method described herein is applicable to other stable GST-fused recombinant proteins.

丝氨酸/苏氨酸激酶PAK1是小gtpase下游细胞骨架重组和癌症相关信号传导的中介。由于PAK1复合物的纯化困难，PAK1的46位残基肽被广泛用于研究PAK1- cdc42信号转导。传统上，这种纯化涉及重组GST-PBD46复合物的多步色谱，每1.5 L培养产生约1 mg。在本研究中，凝血酶裂解后30分钟的热处理步骤沉淀GST，同时将纯PBD46留在溶液中。这一步骤消除了进一步亲和层析和尺寸排除层析步骤的需要。该改进方案生产的蛋白质产量提高6.5倍，纯化处理时间缩短一半，并生产纯度≥95%的肽。质谱、CD、荧光和1H-15N HSQC NMR证实了热纯化PBD46的身份、结构、折叠和与Cdc42的结合。该方法还成功地分离了其他小肽（如ACK1），但不能分离较大的折叠蛋白。这种快速和可扩展的方法促进了肽的生产和生化研究，而不影响结构或功能的完整性。我们相信本文描述的方法适用于其他稳定的gst融合重组蛋白。

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引用次数: 0

Rapid purification of DNA samples from dextran sodium sulfate (DSS) contaminants 脱氧核糖核酸样品中葡聚糖硫酸钠（DSS）污染物的快速纯化。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.ab.2025.116015

Adam Yeh , James Saliba , Volker Blank

Dextran sodium sulfate (DSS) is widely used to model colonic inflammation and colorectal cancer. However, it is a potent inhibitor of DNA polymerase, necessitating its removal prior to downstream analysis. Currently, there is no robust, widely applicable method of removing DSS from DNA samples. Here, we demonstrate that cetyltrimethylammonium bromide (CTAB) buffer enables rapid and efficient removal of DSS from mouse stool DNA. In the absence of CTAB, DSS inhibited PCR reactions at concentrations of 136 nM and above. CTAB effectively removed DSS from DNA samples processed by both phenol-chloroform extraction and column cleanup kits. 700 μL of CTAB was able to remove up to 93 nmol (4 mg) of DSS after one round of cleanup, while two rounds removed up to 465 nmol (20 mg) of DSS. Purified genomic DNA was suitable for subsequent quantitative PCR analysis.

葡聚糖硫酸钠（DSS）被广泛应用于结肠炎症和结直肠癌的模拟。然而，它是DNA聚合酶的有效抑制剂，需要在下游分析之前将其去除。目前，还没有一种强大的、广泛适用的方法从DNA样本中去除DSS。在这里，我们证明十六烷基三甲基溴化铵（CTAB）缓冲液能够快速有效地去除小鼠粪便DNA中的DSS。在没有CTAB的情况下，DSS在136 nM及以上浓度下抑制PCR反应。CTAB可以有效地去除经苯酚-氯仿萃取和柱净化试剂盒处理的DNA样品中的DSS。700 μL CTAB在一次清除后可去除93 nmol （4 mg）的DSS，两次清除后可去除465 nmol （20 mg）的DSS。纯化的基因组DNA适于后续的定量PCR分析。

引用次数: 0

Electrochemical strategies for decoding serotonin using advanced carbonaceous and 5th generation materials: A comprehensive review 利用先进的碳质和第五代材料解码血清素的电化学策略：综合综述。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-10-15 DOI: 10.1016/j.ab.2025.115990

Rimpa Mondal , Sk Faruque Ahmed , Nillohit Mukherjee

Carbonaceous materials are placed in the category of advanced and future materials for their very unique physicochemical attributes like tuneable electronic, electrical and optical properties associated with good chemical reactivity, biocompatibility and high surface to volume ratio that make them good candidates for various device applications including biosensors. Recently, such carbonaceous materials and their composites are being widely used as the electrode materials for enzyme-free electrochemical detection of various bioanalytes. Among the bioanalytes, the neurotransmitters are known to play crucial roles in maintaining proper balance in the human nervous systems. However, there are many challenges like selectivity, sensitivity and rapidity; that are normally faced during the detection of such neurotransmitters using the conventional enzymatic way. Electrochemical biosensors made up of advanced carbonaceous materials could be a single-key answer to all such challenges. Serotonin, a catecholamine group neurotransmitter, also known as the “happy hormone”, is known to control human emotions. So, real-time monitoring of its level in human serum carries immense importance, however; it comes with various challenges. This review article covers the developments of advanced carbonaceous, as well as, the 5th generation materials like Mxenes and related composites in the enzyme-free electrochemical sensing of serotonin since the inception of such materials in electrochemical biosensing. A detailed literature review exhibited that, impressive results, like very low limit of detection (LoD) of 8.0 nM could be achieved using multiwalled carbon nanotube (MWCNT) based electrodes, whereas, nitrogen incorporated graphene quantum dot (N-GQD) modified electrodes can help to reach a notably high sensitivity of 24.0 μAμM⁻¹.cm⁻². Again, carbon spheres (CS) have been established as a good candidate to bring a balance between LoD (4.0 nM) and sensitivity (5.5 μAμM⁻¹.cm⁻²) in combination with metal oxide electrode.

碳质材料因其独特的物理化学特性而被列入先进和未来材料的范畴，如可调谐的电子、电气和光学特性，以及良好的化学反应性、生物相容性和高表面体积比，使其成为包括生物传感器在内的各种设备应用的良好候选者。近年来，这类碳质材料及其复合材料被广泛用作各种生物分析物无酶电化学检测的电极材料。在生物分析物中，神经递质在维持人类神经系统的适当平衡中起着至关重要的作用。然而，有许多挑战，如选择性，灵敏度和速度；在使用传统的酶法检测这些神经递质时，通常会遇到这些问题。由先进的碳质材料组成的电化学生物传感器可能是解决所有这些挑战的一个关键答案。血清素是一种儿茶酚胺类神经递质，也被称为“快乐激素”，它控制着人类的情绪。因此，实时监测其在人类血清中的水平具有极其重要的意义；它伴随着各种各样的挑战。本文综述了先进的碳质材料及第五代材料Mxenes及其复合材料在5 -羟色胺无酶电化学传感领域的研究进展。详细的文献综述表明，使用多壁碳纳米管（MWCNT）电极可以实现8.0 nM的极低检测限（LoD），而氮掺杂石墨烯量子点（N-GQD）修饰电极可以达到24.0 μAμ m- 1 cm-2的高灵敏度。同样，碳球（CS）已被确定为在LoD （4.0 nM）和灵敏度（5.5 μAμM-1）之间取得平衡的良好候选者。Cm-2)与金属氧化物电极结合。

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引用次数: 0

Advances in the detection of azodicarbonamide and the metabolic product semicarbazide 偶氮二甲酰胺及其代谢产物氨基脲的检测研究进展。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-27 DOI: 10.1016/j.ab.2025.116018

Zijuan Miao , Bowen Kan , Pan Liu , Yongming Guo

Azodicarbonamide (ADA) is widely used as a foaming and oxidizing agent in the food and plastic industries. However, long-term exposure to it could cause serious health problems. Its metabolic product, semicarbazide (SEM), has raised health concerns due to findings of potential carcinogenicity and genotoxicity in some studies, though the evidence is context-dependent and sometimes conflicting. Therefore, the development of efficient, sensitive, and rapid detection methods for ADA and SEM is of great significance for food safety assurance. This review systematically summarizes recent advances in detection techniques for ADA and SEM, including high-performance liquid chromatography, capillary electrophoresis, Raman spectroscopy, immunoassays, electrochemical methods, near-infrared spectroscopy, colorimetry, fluorescence, chemiluminescence, photoacoustic, and photothermal detection. Subsequently, the sensitivity, selectivity, applicability, and limitations of these methods are compared and analyzed. Lastly, the challenges and future research trends are discussed. This review will pave the way for the development of advanced sensing strategies for ADA and SEM.

偶氮二甲酰胺（ADA）在食品和塑料工业中广泛用作泡沫剂和氧化剂。然而，长期接触它可能会导致严重的健康问题。由于在一些研究中发现了潜在的致癌性和遗传毒性，其代谢产物氨基脲（SEM）引起了健康问题，尽管证据依赖于环境，有时相互矛盾。因此，开发高效、灵敏、快速的ADA和SEM检测方法对保证食品安全具有重要意义。本文系统地综述了ADA和SEM检测技术的最新进展，包括高效液相色谱、毛细管电泳、拉曼光谱、免疫分析、电化学方法、近红外光谱、比色法、荧光、化学发光、光声和光热检测。随后，对这些方法的灵敏度、选择性、适用性和局限性进行了比较分析。最后，对未来的研究趋势和面临的挑战进行了讨论。本综述将为ADA和SEM的先进传感策略的发展铺平道路。

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引用次数: 0

Structural, optical, and electrochemical characterization of TiO2-x thin films for non-enzymatic amperometric glucose sensing 用于非酶促电流葡萄糖传感的TiO2-x薄膜的结构、光学和电化学表征。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-22 DOI: 10.1016/j.ab.2025.116016

J.K. Olarte Villamizar, M. Zapata Torres, N. Cruz González, G. Silva Galindo

This work presents a novel approach to non-enzymatic glucose sensing based on oxygen-deficient titanium dioxide (TiO_2-x) thin films deposited on metallic titanium substrates. The TiO_2-x films were fabricated via RF magnetron reactive sputtering using a titanium target, and their structural, morphological, optical, and electrochemical properties were comprehensively characterized through X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), UV–Vis spectroscopy, and electrochemical techniques. The electrochemical performance was evaluated using cyclic voltammetry (CV) and chronoamperometry. The CV results revealed that the oxidation in phosphate buffer solution occurs at an applied potential of −0.54 V, increasing with a glucose concentration. Chronoamperometric analysis demonstrated a high sensitivity of 27.55 μA cm⁻² mM⁻¹ and a low detection limit of 0.11 mM. The sensor also exhibited a linear dynamic range from 0.2 mM to 2.4 mM and excellent selectivity against common interfering species, including histidine (His), cysteine (Cys), uric acid (UA), ascorbic acid (AA), and sucrose. These findings highlight the potential of Ti/TiO_2-x electrodes as efficient, stable, and selective platforms for developing non-enzymatic glucose sensors suitable for biomedical and wearable applications, with possible use in detecting glucose concentrations in sweat (0.27 mM–1 mM) and saliva (0.23 mM–1.78 mM).

这项工作提出了一种基于沉积在金属钛衬底上的缺氧二氧化钛（TiO2-x）薄膜的非酶促葡萄糖传感新方法。以钛为靶材，采用射频磁控反应溅射法制备TiO2-x薄膜，并通过x射线衍射（XRD）、扫描电镜（SEM）、x射线光电子能谱（XPS）、紫外可见光谱（UV-Vis）和电化学技术对其结构、形貌、光学和电化学性能进行了全面表征。采用循环伏安法和计时安培法对其电化学性能进行了评价。CV结果表明，磷酸缓冲溶液中的氧化发生在-0.54 V的电位下，随着葡萄糖浓度的增加而增加。该传感器的灵敏度为27.55 μA cm-2·mM-1，检出限为0.11 mM，线性动态范围为0.2 ~ 2.4 mM，对组氨酸（His）、半胱氨酸（Cys）、尿酸（UA）、抗坏血酸（AA）和糖等常见干扰物质具有良好的选择性。这些发现突出了Ti/TiO2-x电极作为高效、稳定和选择性平台的潜力，可用于开发适合生物医学和可穿戴应用的非酶葡萄糖传感器，可能用于检测汗液（0.27 mM-1 mM）和唾液（0.23 mM-1.78 mM）中的葡萄糖浓度。

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引用次数: 0

Glutaminase I and glutamine transaminase–ω-amidase pathways in colorectal cancer: Metabolic reprogramming and emerging therapeutic strategies 谷氨酰胺酶I和谷氨酰胺转氨酶ω-氨基酶途径在结直肠癌中的作用：代谢重编程和新的治疗策略。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-10-10 DOI: 10.1016/j.ab.2025.115989

Sarah F. Voor, Arthur J.L. Cooper, John T. Pinto

Colorectal cancer (CRC) cells exhibit a pronounced dependence on l-glutamine to support anabolic growth, redox balance, and mitochondrial metabolism, a phenomenon known as “glutamine addiction.” The canonical glutaminase I pathway is mediated by a liver type glutaminase isozyme (LGA; GLS1), a kidney type glutaminase isozyme (KGA; GLS2), and a shortened form (glutaminase C, GAC). GLS1 and GLS2 convert glutamine to glutamate and subsequently to α-ketoglutarate (α-KG) by glutamate dehydrogenase, fueling the TCA cycle. GLS1 inhibitors, such as CB-839 (Telaglenastat), are under clinical evaluation and shows promise in treatment of CRC, particularly in combination therapies. In addition to the canonical pathway, the glutamine transaminase–ω-amidase (GTωA) pathway, a noncanonical route involving transamination of glutamine to α-ketoglutaramate (KGM) by KYAT1/2 and subsequent hydrolysis by ω-amidase (NIT2), offers metabolic flexibility under hypoxic or nutrient-limited conditions. Preclinical studies suggest that GTωA may compensate for GLS1 inhibition, contributing to therapeutic resistance. This review explores the dual roles of glutamine metabolism in CRC, emphasizing the GTωA pathway as a potentially targetable metabolic route that may contribute to therapeutic resistance. While GLS1 inhibitors are under clinical evaluation, emerging evidence suggests that dual targeting of both pathways may enhance treatment efficacy by overcoming metabolic compensation. Understanding the regulatory mechanisms driving the “glutamine shift” between these pathways is critical for developing effective metabolic interventions in CRC.

结直肠癌（CRC）细胞明显依赖l -谷氨酰胺来支持合成代谢生长、氧化还原平衡和线粒体代谢，这种现象被称为“谷氨酰胺成瘾”。典型谷氨酰胺酶I途径由肝型谷氨酰胺酶同工酶（LGA; GLS1）、肾型谷氨酰胺酶同工酶（KGA; GLS2）和缩短形式（谷氨酰胺酶C， GAC）介导。GLS1和GLS2通过谷氨酸脱氢酶将谷氨酰胺转化为谷氨酸，随后转化为α-酮戊二酸（α-KG），促进TCA循环。GLS1抑制剂，如CB-839 (Telaglenastat)，正在进行临床评估，并显示出治疗结直肠癌的希望，特别是在联合治疗中。除了典型途径外，谷氨酰胺转氨酶-ω-氨基酶（gt -ω-a）途径是一种非典型途径，涉及由KYAT1/2将谷氨酰胺转氨酶转化为α-酮戊二酸盐（KGM），随后被ω-氨基酶（NIT2）水解，在缺氧或营养受限条件下提供代谢灵活性。临床前研究表明，GTωA可能补偿GLS1抑制，有助于治疗耐药。这篇综述探讨了谷氨酰胺代谢在结直肠癌中的双重作用，强调GTωA途径可能是一个潜在的靶向代谢途径，可能有助于治疗耐药。虽然GLS1抑制剂仍处于临床评估阶段，但新出现的证据表明，双重靶向这两种途径可能通过克服代谢代偿来提高治疗效果。了解这些途径之间驱动“谷氨酰胺转移”的调节机制对于开发有效的CRC代谢干预措施至关重要。

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引用次数: 0

Systematic errors in isothermal titration calorimetry: The role of feedback power and effects of mixing and diffusion on concentrations 等温滴定量热法中的系统误差：反馈功率的作用以及混合和扩散对浓度的影响。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-11 DOI: 10.1016/j.ab.2025.116010

Žiga Medoš , Marija Bešter-Rogač , Epameinondas Leontidis , Joel Tellinghuisen

Using experiments based on the NaCl dilution calibration method, we examine several previously unstudied instrumental effects that can lead to systematic errors in isothermal titration calorimetry (ITC). We confirm earlier results that power feedback can affect estimated heats and find further that it can also affect cell temperature and introduce temperature “noise.” To estimate effects from loss of titrant from the syringe through diffusion, and concentration changes in the cell from mixing with the overflow volume, we conduct experiments with injections separated by time intervals Δt from 8 to 60 min, giving run durations from 6 h to over 2 days. Because the effects are small, we have developed a global least-squares fitting algorithm to analyze 20 datasets simultaneously, from which three parameters governing diffusion and mixing (“leakage”) are estimated, along with calibration parameters for heat and cell volume. Expressed as an effective volume loss from the syringe, Δv_dif is found to be of order ∼0.03 μL per injection for NaCl into water. Its effect is predicted to be negligible in many ITC applications. The effects from leakage are more significant but their neglect is still predicted to affect both NaCl calibration and 1:1 binding results by ∼1 %, which is marginally significant for calibration but seldom so for 1:1 binding. The calibration results for the cell volume V₀ are particularly sensitive to temperature and to the literature source for the relative molar enthalpy function for NaCl(aq).

利用基于NaCl稀释校准方法的实验，我们研究了几种以前未研究过的可能导致等温滴定量热法（ITC）系统误差的仪器效应。我们证实了先前的结果，即功率反馈可以影响预估的热量，并进一步发现它也可以影响电池温度并引入温度“噪声”。为了估计注射器中滴定剂因扩散而损失的影响，以及细胞中因与溢出液混合而产生的浓度变化，我们进行了注射实验，注射间隔时间为Δt，间隔时间为8至60分钟，持续时间为6小时至2天以上。由于影响很小，我们开发了一种全局最小二乘拟合算法来同时分析20个数据集，从中估计控制扩散和混合（“泄漏”）的三个参数，以及热量和细胞体积的校准参数。以注射器的有效体积损失来表示，Δvdif发现每次注射NaCl到水中的量级为~ 0.03 μL。预计其影响在许多ITC应用中可以忽略不计。泄漏的影响更为显著，但它们的忽视仍预计会对NaCl校准和1:1结合结果产生约1%的影响，这对校准有轻微影响，但对1:1结合的影响很少。细胞体积V0的校准结果对温度和NaCl（aq）的相对摩尔焓函数的文献来源特别敏感。

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引用次数: 0

StackAPP: Advancing autophagy protein identification with ensemble learning 利用集成学习推进自噬蛋白鉴定。

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry

Pub Date : 2026-01-01 Epub Date: 2025-09-18 DOI: 10.1016/j.ab.2025.115981

Munem Shahriar Shoyshob , Kusay Faisal Al-Tabatabaie , Lway Faisal Abdulrazak , Md. Ashikur Rahman , Md. Mamun Ali , Sobhy M. Ibrahim , Kawsar Ahmed , Francis M. Bui , Mohammad Ali Moni

Autophagy is an important cell process that may be critical for various physiological activities as well as maintenance of the cellular bioenergetic and metabolic homeostasis. Identifying the proteins involved in autophagy is essential for understanding autophagy pathways and developing treatments for autophagy-related disorders. This work introduces an innovative approach to the prediction of autophagy proteins that involves the integration of stacking classifiers with the feature fusion of Amphiphilic Pseudo Amino Acid Composition and Amino Acid Composition. Initially, protein sequences are used to extract Amphiphilic Pseudo Amino Acid Composition and Amino Acid Composition features. The complementary data collected by Amphiphilic Pseudo Amino Acid Composition and Amino Acid Composition are then integrated using a feature fusion technique. Stacking classifiers combines multiple base classifiers to improve predictive performance, using the fused features as input. The proposed method proves its efficacy in the identification of autophagy proteins by achieving an impressive accuracy of 0.9606 and the Matthews correlation coefficient (MCC) of 0.9241 on the independent test. Further, our methodology is better than the standard methods in terms of predictive accuracy, as evidenced through comparative analysis. Overall, the current study provides a realistic model for the prediction of autophagy proteins with prospects for use in the protein prediction field as well as the field of bioinformatics and biomedical to enhance future research directions.

自噬是一个重要的细胞过程，它可能对各种生理活动以及维持细胞的生物能量和代谢稳态至关重要。确定参与自噬的蛋白质对于理解自噬途径和开发自噬相关疾病的治疗方法至关重要。这项工作引入了一种预测自噬蛋白的创新方法，该方法涉及将堆叠分类器与两亲性伪氨基酸组成和氨基酸组成的特征融合。最初，蛋白质序列用于提取两亲性伪氨基酸组成和氨基酸组成特征。然后使用特征融合技术对两亲性伪氨基酸组成和氨基酸组成收集的互补数据进行整合。叠加分类器结合多个基分类器，使用融合的特征作为输入来提高预测性能。该方法鉴定自噬蛋白的准确性达到0.9606，独立检测的Matthews相关系数（MCC）达到0.9241，证明了该方法的有效性。此外，通过对比分析，我们的方法在预测准确性方面优于标准方法。总的来说，本研究为自噬蛋白的预测提供了一个现实的模型，在蛋白质预测领域以及生物信息学和生物医学领域都有应用前景，以加强未来的研究方向。

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引用次数: 0