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Gene therapy, physiological applications, problems and prospects - a review 基因治疗的生理应用、问题及展望综述
Pub Date : 2019-09-06 DOI: 10.4314/ARI.V16I2
G. Ugwu, J. V. Egbuji, Laureta Chinagorom Okanya, J. N. Omeje, Joseph Effiong Eyo
Gene therapy can be defined as the use of DNA as a pharmaceutical agent to treat disease. It is also an experimental medical treatment that manipulates a gene or genes within cells in order to produce proteins that change the function of those cells. The physiological applications, problems and prospects of gene therapy are reviewed in this study. The different types of gene therapy such as germline gene therapy, somatic gene therapy and chimeraplasty gene therapy are discussed. Polymerase chain reaction (PCR), nanoparticles, sonoporation, electroporation and gene gun are the techniques used in gene therapy. Polymerase chain reaction (PCR) is used in medical and biological research. Nanoparticles have been widely used in the field of drug and gene delivery to target cells. Sonoporation allows uptake of large molecules of DNA into the cell, in a process called cell transformation. Electroporation is highly efficient for the introduction of foreign genes in tissue culture cells, in tumor treatment and cell-based therapy. A gene gun is a device for injecting cells with genetic information to plant cells. Gene therapy is applied in medicine, agriculture, loss and gain of function, tracking and expression studies. Some problems bedeviling gene therapy include insertional mutagenesis, mutagenic disorders, problem of viral vectors, immune response etc. Gene therapy has the potential to eliminate and prevent hereditary diseases such as cystic fibrosis and is a possible cure for Alziehmer’s disease and cancer, enhance agricultural productivity of farm animals, and in the production of genetically modified animals (GMOs) which will further help in medical and biomedical research. Keywords: Gene therapy, Chimeraplasty, Nanoparticles, Sonoporation, Electroporation, Gene gun, Polymerase chain reaction
基因治疗可以定义为使用DNA作为药物来治疗疾病。它也是一种实验性的医学治疗,通过操纵细胞内的一个或多个基因来产生改变这些细胞功能的蛋白质。本文就基因治疗的生理应用、存在的问题及前景进行综述。讨论了不同类型的基因治疗,如生殖细胞基因治疗、体细胞基因治疗和嵌合成形术基因治疗。聚合酶链反应(PCR)、纳米颗粒、声孔、电孔和基因枪是基因治疗中常用的技术。聚合酶链反应(PCR)用于医学和生物学研究。纳米颗粒已广泛应用于药物和基因靶向递送领域。声波穿孔允许大分子DNA进入细胞,这一过程被称为细胞转化。电穿孔在组织培养细胞中引入外源基因、肿瘤治疗和细胞基础治疗中具有很高的效率。基因枪是一种将带有遗传信息的细胞注射到植物细胞中的装置。基因治疗应用于医学、农业、功能的丧失和获得、跟踪和表达研究。困扰基因治疗的问题包括插入性突变、诱变性疾病、病毒载体问题、免疫反应等。基因疗法具有消除和预防诸如囊性纤维化等遗传性疾病的潜力,并且可能治愈阿尔茨海默病和癌症,提高农场动物的农业生产力,以及生产转基因动物,这将进一步有助于医学和生物医学研究。关键词:基因治疗,嵌合成形术,纳米粒子,超声穿孔,电穿孔,基因枪,聚合酶链反应
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引用次数: 2
Determination of the concentration of some mineral elements and pollen spectra of Apis mellifera L. honeys from different locations in Nigeria 尼日利亚不同产地蜜蜂部分矿质元素浓度及花粉谱测定
Pub Date : 2019-05-20 DOI: 10.4314/ARI.V16I1
R. Njokuocha, Kingsley Ikechukwu Dim, Ogochukwu Kosi Onyejekwe, Victor Uchenna Nwokorie
Honey is a commonly preferred natural food product because it is composed of complex organic and inorganic substances that impart nutritional and therapeutic properties to it. Because the concentrations of these substances vary with location and affected by anthropogenic factors, it becomes pertinent to determine the level of occurrence of this substances necessary for quality assessment of honey. The aims of this study were to ascertain the concentrations of some major mineral elements (K, Ca, Mg, Na), some trace elements (Mn, Cr, Cu, Fe, Zn, Ni) as well as the pH and pollen spectra of honey samples sourced from six locations in Nigeria. The mineral elements which were analysed with Atomic Absorption Spectrometer (AAS) showed that the most abundant minerals were Ca and K, while Fe and Ni were the most abundant trace elements. The low level of the trace elements shows that the source environments of the honeys are uncontaminated. The results also showed that the honey samples were acidic with a range of 3.5 ± 0.02 – 4.72 ± 0.01 acidic level. Pollen analysis showed that the honeys were multiflora indicating that they were formed from multiple flora. Keywords: Mineral elements, Trace elements, pH, pollen, Multiple flora, Multiflora honey
蜂蜜是一种普遍首选的天然食品,因为它由复杂的有机和无机物质组成,赋予它营养和治疗特性。由于这些物质的浓度随地点和人为因素的影响而变化,因此确定这些物质的出现水平对蜂蜜的质量评估是必要的。本研究的目的是确定尼日利亚六个地点蜂蜜样品的一些主要矿物元素(K、Ca、Mg、Na)和一些微量元素(Mn、Cr、Cu、Fe、Zn、Ni)的浓度以及pH值和花粉谱。原子吸收光谱(AAS)分析表明,矿物元素含量最高的是Ca和K,微量元素含量最高的是Fe和Ni。蜂蜜中微量元素含量低,说明蜂蜜源环境未受污染。结果还表明,蜂蜜呈酸性,酸度范围为3.5±0.02 ~ 4.72±0.01。花粉分析表明,这些蜂蜜具有多区系,表明它们是由多个区系形成的。关键词:矿质元素,微量元素,pH值,花粉,多花群,多花蜜
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引用次数: 10
Comparative study of the bacterial load and species diversity in the African catfish ( Clarias gariepinus ) cultured in contrasting aquaculture tanks in Uyo, Nigeria 尼日利亚乌约不同养殖池中非洲鲶鱼(Clarias gariepinus)细菌载量和物种多样性的比较研究
Pub Date : 2019-01-10 DOI: 10.4314/ARI.V16I3
M. U. Effiong, Imoh Nathaniel Isaac
Comparative study on bacterial load and diversity on the intestine, skin and gills of cultured Clarias gariepinus from concrete and tarpaulin tanks were investigated. Determination of bacterial loads, species characterization and composition in fish parts were done using standard microbiological procedures. Results of the assay showed high bacterial count in intestine and gills of fish from both culture tanks. Heterotrophic bacteria count ranged from 1.7 x 10 4 cfu/ml on skin of catfish cultured in tarpaulin tank to 2.6 x 10 4 cfu/ml in gills of catfish from both systems, while the total coliform count ranged from 1.2 x 10 4 cfu/ml in the gills to 3.9 x 10 4 cfu/ml in the intestine. The Salmonella count was higher in the intestine of catfish in both systems, while the highest vibrio counts of 4.2 – 4.6 x 10 4 cfu/ml was recorded in the gills of catfish from tarpaulin tank. In both culture systems, Pseudomonas, Salmonella and Escherichia coli, were not observed on fish skin. The Bacterial organisms isolated included: E scherichia coli, Salmonella enterica, Staphylococcus epidermidis, Vibrio anguillarum, Pseudomonas fluorescens, Serratia liquefaciens, Bacillus licheniformis, Shigella sonnei, Lactobacillus plantarum, Enterobacter aerogenes, Klebsiella pneumoniae and Proteus volgaris. S. epidermidis accounted for the highest frequency of occurrence (75.0 %) in concrete tank, while K. pneumoniae had the least frequency of occurrence (2.0 %) in tarpaulin tank. The bacterial flora recovered composed of potential pathogenic organisms of public health interest. Keywords : Aquaculture, Bacterial load, Catfish, Concrete and tarpaulin tanks
对比研究了混凝土池和防水油布池培养的加里平Clarias gariepinus肠道、皮肤和鳃上的细菌负荷和多样性。采用标准微生物学程序测定鱼体内的细菌负荷、菌种特征和组成。实验结果显示,两个培养槽的鱼的肠道和鳃中细菌数量都很高。在篷布池中培养的鲶鱼皮肤上的异养细菌数量为1.7 × 10.4 cfu/ml,在两种系统中培养的鲶鱼鳃上的异养细菌数量为2.6 × 10.4 cfu/ml,而在鳃上的总大肠菌数量为1.2 × 10.4 cfu/ml,在肠道中为3.9 × 10.4 cfu/ml。两种系统的鲶鱼肠道中沙门氏菌计数均较高,而篷布池鲶鱼鳃中弧菌计数最高,为4.2 - 4.6 × 10.4 cfu/ml。在两种培养系统中,鱼皮上均未发现假单胞菌、沙门氏菌和大肠杆菌。分离的细菌包括:大肠杆菌、肠炎沙门氏菌、表皮葡萄球菌、鳗弧菌、荧光假单胞菌、液化沙雷菌、地衣芽孢杆菌、索尼氏志贺氏菌、植物乳杆菌、产气肠杆菌、肺炎克雷伯菌和伏尔加变形杆菌。混凝土罐体中表皮棘球蚴的发生率最高(75.0%),篷布罐体中肺炎克雷伯菌的发生率最低(2.0%)。恢复的细菌菌群由公共卫生利益的潜在致病生物组成。关键词:水产养殖;细菌负荷;鲶鱼
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引用次数: 12
INSECTICIDE SUSCEPTIBILITY STATUS OF AEDES AEGYPTI IN UMUDIKE, IKWUANO LGA ABIA STATE, NIGERIA 尼日利亚伊库瓦诺伊加比亚州umudike地区埃及伊蚊杀虫剂敏感性调查
Pub Date : 2018-09-21 DOI: 10.4314/ARI.V15I3
O. M. Ukpai, C. M. Ekedo
Control of Anopheles vector is effective in halting malaria. Entrapping them by deceit with semiochemicals they must respond to could be sustainable control approach but poverty and other impediments prevent use of such luring strategy in Sub Saharan Africa. Elaeis guineensis could be alternative accessible and cheap unexplored source of semiochemicals for mosquitoes control. We evaluated the response of Anopheles mosquitoes to categories of palm wine. Anopheles larvae were selected from mosquito juveniles collected from egbaite. At emergence, female mosquitoes were subjected to a 2-way olfactometer to evaluate their responses to odours from 4 categories of palm wines. Fresh up-palm wine attracted 50.00 ± 5.77% of mosquitoes, its sub-category, ebacha up-wine, attracted 45 %. Fresh down-wine attracted 70 % of vectors as against 68.33 % of ebacha down-wine. The foregoing categories compared to the control attracted significantly more mosquitoes (respectively, p = 0.007; p = 0.005; p = 0.002; p = 0.001). Comparison of fresh up-wine with fresh downwine showed that significantly more mosquitoes visited down-wine (t = - 5.060, p = 0.007). Ebacha up-wine and ebacha down-wine comparison revealed that significantly more mosquitoes visited ebacha down-wine (t = - 4.950, p = 0.008). The preference indices calculated for the 4 categories of palm wine ranged from 57.27 ± 14.90 to 85.98 ± 7.73. Palm wines are attractive to Anopheles mosquitoes. Down-wine was more effective attractant. Up wine lost its activity faster than down-wine. Palm wines could sustainably be employed in malaria vector control programmes.Keywords: Malaria/mosquito control, Anopheles, Elaeis guineensis, Semiochemicals, Palm wine, 2-way olfactometer
控制疟蚊媒介是遏制疟疾的有效手段。用它们必须作出反应的化学物质诱骗它们可能是可持续的控制方法,但贫困和其他障碍阻碍了在撒哈拉以南非洲使用这种引诱战略。几内亚伊蚊可能是另一种可获得和廉价的未开发的蚊虫控制半化学物质来源。研究了按蚊对棕榈酒种类的反应。按蚊幼虫选自采自卵贝的幼蚊。在雌蚊羽化时,用双路嗅觉仪评估雌蚊对4种棕榈酒气味的反应。鲜棕榈酒诱蚊率为50.00±5.77%,其亚类巴巴查酒诱蚊率为45%。新鲜羽绒酒吸引了70%的病媒,而巴哈羽绒酒吸引了68.33%的病媒。与对照组相比,上述类别吸引的蚊子数量显著增加(p = 0.007;P = 0.005;P = 0.002;P = 0.001)。鲜上酒与鲜下酒的蚊虫访蚊率差异有统计学意义(t = - 5.060, p = 0.007)。埃巴查上酒区和埃巴查下酒区蚊虫访蚊量差异有统计学意义(t = - 4.950, p = 0.008)。4类棕榈酒的偏好指数取值范围为57.27±14.90 ~ 85.98±7.73。棕榈酒对按蚊有吸引力。唐酒引诱效果较好。升酒比降酒更快失去活性。棕榈酒可以可持续地用于疟疾病媒控制规划。关键词:疟疾/蚊虫防治,按蚊,几内亚依蚊,化学制品,棕榈酒,双向嗅觉仪
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引用次数: 6
Preliminary study on the prevalence of gastrointestinal parasites of pigs managed and slaughtered in Umuahia north local government area of Abia State, Nigeria 尼日利亚阿比亚州Umuahia北部地方政府区管理和屠宰猪胃肠道寄生虫流行情况的初步研究
Pub Date : 2018-07-21 DOI: 10.4314/ARI.V15I2
A. Amadi, P. C. Ubiaru, G. Ugagu, B. N. Ibediugha, I. A. Obeagu, Ann Njikeonye
Fishes are known for their abilities to concentrate heavy metals in their muscles and since they play important role in human nutrition, they need to be carefully screened to ensure that unnecessary high level of some toxic trace metals are not being transfer to man through their consumption. The study was to evaluate heavy metals (chromium, lead, iron and cadmium) in imported and local species of shrimps, crabs and fish using Atomic Absorption Spectroscopy. The selected imported species were horse mackerelkote fish (Trachurus trachurus), mackerel - titus (Scomber scrombus), croaker (Pseudotolithus typus), smooth swim crab (Portunus validus) and pink shrimp (Farfantepenaeus notialis) while the local species used were; Sole fish (Cynoglossus senegalensis), Tilapia (Oreochromis niloticus), brackish water catfish (Chrysichthys nigrodigitatus), freshwater catfish (Clarias gariepinus), lagoon crab (Callinectes amnicola) and freshwater prawn (Macrobrachium macrobrachion). It was observed that there was no significant difference in the metals (Cd, Pb and Cr) between the imported and local fish species and between imported and local crustacean species. However, these metals concentrated more in the local fish species, O. niloticus (1.846 ± 1.00 mg/100g). The mean heavy metals concentration occurred in the following decreasing order: Fe > Cd > Pb > Cr for all the samples. The mean concentrations of the analyzed heavy metals in the samples were below the maximum permissible limits as recommended by the International Atomic Energy Agency (IAEA), United Nations Environment Program (UNEP) and Federal Environmental Protection Agency (FEPA) for heavy metals in fish.Keywords: Fish, Shrimp, Crab, Heavy metal, Chromium, Lead, Iron
鱼类以其将重金属集中在肌肉中的能力而闻名,由于它们在人体营养中起着重要作用,因此需要对它们进行仔细筛选,以确保某些不必要的高水平有毒微量金属不会通过食用而转移给人体。这项研究是利用原子吸收光谱法评估进口和本地虾、蟹和鱼的重金属(铬、铅、铁和镉)。选用的进口品种有马鲛鱼(Trachurus Trachurus)、竹鲛鱼(Scomber scrombus)、黄花鱼(Pseudotolithus typus)、平游蟹(Portunus validus)和粉虾(Farfantepenaeus notialis),选用的本地品种有;比目鱼(Cynoglossus senegalensis)、罗非鱼(Oreochromis niloticus)、半咸水鲶鱼(Chrysichthys nigrodigitatus)、淡水鲶鱼(Clarias gariepinus)、泻湖蟹(Callinectes amnicola)和淡水对虾(Macrobrachium macrobrachion)。结果表明,进口鱼类和本地鱼类以及进口甲壳类动物的镉、铅和铬含量均无显著差异。然而,这些金属在当地鱼类O. niloticus中的浓度更高(1.846±1.00 mg/100g)。所有样品的平均重金属浓度依次递减:Fe > Cd > Pb b> Cr。分析的样本中重金属的平均浓度低于国际原子能机构、联合国环境规划署和联邦环境保护署对鱼类重金属建议的最高允许限度。关键词:鱼、虾、蟹、重金属、铬、铅、铁
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引用次数: 5
Influence of enzyme supplementation on rabbits fed rumen liquor with poultry waste fermented cassava peels based diets 饲粮中添加酶对家兔瘤胃废液和发酵木薯皮的影响
Pub Date : 2018-01-01 DOI: 10.4314/ARI.V15I1
O. Oloruntola
The effect of increasing the optimum replacement level of maize with rumen liquor fermented cassava peels (RLFCP) with enzyme (E) supplementation in rabbit diets was evaluated in 56-day feeding trial using a 2 x 3 factorial experimental design. The E supplementation significantly increased (p 0.05) in the rabbit FLW, TWG and feed conversion ratio (FCR). However, RLFCP inclusion at various levels significantly increased (p<0.05) the daily feed intake (DFI). The slaughtered weight, skin, head and limb, kidneys, gall bladder and gastro intestinal tract were significantly affected (p<0.05) by dietary RLFCP levels. RLFCP inclusion significantly increased (p<0.05) packed cell volume (PCV), mean cell volume (MCV) and lymphocyte (LYM) levels. Enzyme x RLFCP was significant increased (p<0.05) the number of red blood cells. Equally, the concentrations of albumin, globulin, alkaline phosphatase, amylase and glucose were significantly affected (p<0.001) by the level of RLFCP in the diets such that replacement level of maize for RLFCP lead to increase in albumin and glucose, alkaline phosphate, while the effect of the replacement levels did not follow a particular trend for amylase and glucose. Addition of multi-enzyme at 0.35 g/kg level led to improvement in weight gain of the rabbits. Keywords: Fermented cassava peels, Rabbits, Enzyme supplementation, Blood profiles, Health status
采用2 × 3因子试验设计,通过添加酶(E)提高瘤胃白酒发酵木薯皮(RLFCP)在家兔饲粮中的最佳替代水平,评价其饲喂56 d的效果。饲粮中添加E显著提高了家兔FLW、TWG和饲料系数(FCR) (p 0.05)。不同水平的RLFCP均显著提高了日采食量(DFI) (p<0.05)。饲粮RLFCP水平对肉鸡屠宰体重、皮肤、头肢、肾脏、胆囊和胃肠道有显著影响(p<0.05)。RLFCP包埋显著提高了细胞堆积体积(PCV)、平均细胞体积(MCV)和淋巴细胞(LYM)水平(p<0.05)。酶x RLFCP显著增加红细胞数量(p<0.05)。饲粮中RLFCP水平对白蛋白、球蛋白、碱性磷酸酶、淀粉酶和葡萄糖浓度也有显著影响(p<0.001),玉米替代RLFCP水平会导致白蛋白、葡萄糖、碱性磷酸盐浓度升高,而对淀粉酶和葡萄糖的影响没有特别的趋势。添加0.35 g/kg水平的复合酶可改善家兔增重。关键词:发酵木薯皮,兔子,酶补充,血液特征,健康状况
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引用次数: 3
Bedbug ( Cimex lectularius ) infestation and its control practices in two selected settlements in Ilorin, Kwara State, Nigeria 尼日利亚夸拉州伊洛林两个选定住区臭虫(Cimex lectularius)侵染情况及其防治措施
Pub Date : 2017-12-06 DOI: 10.4314/ARI.V14I3
A. Oduola, A. Omotayo, O. Adelaja, A. Obembe
Bedbugs are cosmopolitan insects of public health importance. Their characteristic biting and blood feeding activities result in both physical symptoms and psychological distress in affected hosts. This study investigated the incidence of bedbug and control practices among students and indigenes living in two different settlements in Ilorin, Nigeria. Bedbug were surveyed and collected from household materials from fifty apartments randomly selected from each of the new and old student hostels and family houses in Tanke and  Oja-Oba area of Ilorin. Bedbugs collected were persevered in 70 % alcohol and identified using taxonomic keys. A structured questionnaire was used to obtain information on respondent’s demography, perception about bedbug infestation and control practices in sampled houses in the study areas. Sampled data were analysis using percentages.  Results from the survey revealed that the number of respondents with knowledge of bedbug was not significant (p>0.05) compared with those without. Family houses in indigenous Oja-Oba study location were the only site with bed-bug infestation. Higher numbers of bedbugs were collected on mattresses. Out of the 200 respondents, 20 (10 %) employed the use of chemical method in bedbug control and precisely, 12 (60 %) of these use a locally made formulation named otapiapia. The intensity of infestation in Oja-Oba and insignificant knowledge of bedbug among the respondents suggest the need to raise the level of public awareness and also canvass for an improved living conditions in the affected areas .
臭虫是具有公共卫生重要性的世界性昆虫。它们特有的咬人和吸血活动导致受感染宿主的身体症状和心理困扰。本研究调查了尼日利亚伊洛林两个不同定居点的学生和土著居民的臭虫发病率和控制措施。在Ilorin Tanke和Oja-Oba地区的新老学生宿舍和家庭住宅中随机选择了50套公寓,调查和收集了臭虫。收集的臭虫在70%的酒精中保存,并使用分类键进行鉴定。采用结构化问卷调查的方式,了解调查对象的人口学信息、对研究地区抽样房屋臭虫侵害的认知和防治措施。抽样数据使用百分比进行分析。调查结果显示,与不了解臭虫知识的受访者相比,了解臭虫知识的受访者人数无统计学意义(p>0.05)。土著Oja-Oba研究地点的家庭住宅是唯一有臭虫侵扰的地点。床垫上的臭虫数量较多。在200个答复者中,20个(10%)采用化学方法控制臭虫,准确地说,12个(60%)使用当地生产的名为otapiapia的制剂。Oja-Oba的虫害严重程度以及受访者对臭虫知之甚少表明有必要提高公众认识水平,并为改善受影响地区的生活条件进行游说。
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引用次数: 5
Factors affecting feed intake in cultured fish species: A review 影响养殖鱼类采食量的因素综述
Pub Date : 2017-06-23 DOI: 10.4314/ARI.V14I2
O. J. Eriegha, P. Ekokotu
Feed expense constitutes the bulk of the whole cost of production in any intensive aquaculture setup in Nigeria. Consequently, feed and feeding stratagems are of paramount interest for feed utilization as well as profit maximization. However, the paucity of documents on the factors affecting feed intake in cultured fish is negatively influencing the efficiency of fish production from aquaculture. Hence, the major factors influencing feed intake by cultured fish are discussed. These factors include management practices, environment conditions, feed quality, inherent genetic factor and physiological condition of the fish. The review would help to increase basic knowledge on the nutrition (with emphasis on feed intake) of aquaculture species for improved management and productivity.
在尼日利亚任何集约化水产养殖机构中,饲料费用占生产总成本的大部分。因此,饲料和饲养策略对饲料利用和利润最大化至关重要。然而,关于养殖鱼类采食量影响因素的文献缺乏,这对水产养殖鱼类的生产效率产生了负面影响。因此,本文讨论了影响养殖鱼类采食量的主要因素。这些因素包括管理措施、环境条件、饲料质量、内在遗传因素和鱼的生理状况。这项审查将有助于增加对水产养殖品种营养(重点是采食量)的基本认识,以改进管理和生产力。
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引用次数: 36
EVALUATION OF ANAESTHETIC CHARACTERISTICS OF PROPOFOL IN NON-PREMEDICATED RABBITS WITH EXPERIMENTALLY INDUCED POST RENAL UNILATERAL URETERAL OBSTRUCTION 异丙酚对实验性肾后单侧输尿管梗阻家兔非预用药的麻醉特性评价
Pub Date : 2017-01-08 DOI: 10.4314/ARI.V14I1
V. Oleforuh-Okoleh, Romanus Francis Kurutsi, Hanson Modhiochi Ideozu
This study was carried out to investigate the anaesthetic characteristics of propofol in rabbits with unilateral ureteral obstruction. Rabbits in two groups (B and C) were anaesthetized respectively with 10 mg/kg propofol intravenously (IV) on days 7 and 14 post unilateral ureteral obstruction (UUO). Healthy rabbits in group A served as the control group and were anaesthetized with propofol (10 mg/kg, IV). Duration of anaesthesia obtained in the control group was significantly (p 0.05) while white blood cell counts of rabbits decreased PPI. Total serum protein (TSP) and blood urea nitrogen levels of the three groups of rabbits increased PPI though not significantly (p>0.05) PPI. Serum potassium, chlorine and bicarbonate levels of rabbits in all the three study groups increased PPI. Propofol at the dose used in this study induced rapid induction and recovery from anaesthesia, adequate depth of anaesthesia with no untoward haematologic and serum biochemical effects in UUO rabbits. However it’s marked respiratory depressant effect and ability to increase serum potassium levels may preclude its use in advanced UUO.
本研究探讨异丙酚对单侧输尿管梗阻家兔的麻醉特性。B组和C组分别于单侧输尿管梗阻(UUO)后第7天和第14天静脉注射10 mg/kg异丙酚麻醉。A组健康家兔为对照组,以异丙酚(10 mg/kg,静脉)麻醉,对照组麻醉时间显著延长(p 0.05),白细胞计数明显降低PPI。三组家兔血清总蛋白(TSP)和血尿素氮水平均升高了PPI,但未显著升高(p>0.05)。三个研究组兔血清钾、氯和碳酸氢盐水平均升高PPI。本研究中使用的剂量异丙酚能使UUO家兔快速诱导和恢复麻醉,麻醉深度足够,无不良血液学和血清生化影响。然而,它明显的呼吸抑制作用和提高血清钾水平的能力可能使其无法用于晚期UUO。
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引用次数: 17
MORPHOLOGICAL CHARACTERISTICS OF THE SMALL INTESTINE OF THE AFRICAN PIED CROW (Corvus albus) 非洲斑鸦小肠的形态特征
Pub Date : 2017-01-02 DOI: 10.4314/ARI.V7I1
U. Igwebuike, U. U. Eze
The gross and histo-morphological characteristics of the small intestine of the African pied crow are described in this study. The general gross morphology of the small intestine of these birds follows the basic model of the avian small intestine, except that the jejunum of the African pied crow was organized in the form of cone-shaped spiral coils. The cone-shaped jejunum had centripetal coils, a sigmoid flexure and centrifugal coils. The histology of the wall of the small intestine of the African pied crow revealed four layers of tissues; the mucosa, submucosa, muscularis and serosa. Morphological modification of these tissue layers was observed, and is thought to be a necessary adaptation for rapid breakdown and absorption of food.
本研究描述了非洲斑鸦小肠的大体和组织形态学特征。这些鸟类小肠的大体形态遵循鸟类小肠的基本模型,除了非洲斑鸦的空肠以锥形螺旋线圈的形式组织。锥形空肠有向心圈、乙状形弯曲和离心圈。非洲斑乌鸦小肠壁的组织学显示有四层组织;粘膜,粘膜下层,肌层和浆膜。观察到这些组织层的形态改变,被认为是快速分解和吸收食物的必要适应。
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引用次数: 6
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Animal Research International
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