This study compared the cholesterol levels and shell pigments (protoporphyrin and biliverdin) of chicken (conventional and organic), quail, pheasant, and goose eggs. The material for the study was chicken (organic system - Lohmann Brown and conventional system - HyLine Brown) eggs, quail ( Coturnix coturnix japonica ), goose (local), and pheasant ( Phasianus colchicus ) eggs homogeneously selected with a subjective scoring. High-performance liquid chromatography with photodiode array detection was used to analyze the samples (HPLC-PDA). There were no significant differences in the yolk cholesterol content of eggs between species. Based on mg/g of yolk, different poultry species had comparable amounts of cholesterol. Quail eggshells contained significantly more protoporphyrin (81.92 M/g) than chicken (conventional-organic) and pheasant eggshells (P<0.01), but conventional chicken eggshells contained less protoporphyrin (10.73 M/g) than other species (P<0.01). Biliverdin was found only in the eggshells of quail (2.83 M/g) and pheasant (1.02 M/g) (P<0.01). It was observed that white shelled goose eggs had no detectable pigment. Research is required to elucidate the role of diet, age, stressor, strain, and housing systems on protoporphyrin and biliverdin pigment concentrations and cholesterol in table eggs and breeder eggs production.
{"title":"A comparative study on egg cholesterol contents and eggshell protoporphyrin and biliverdin pigments of different poultry species","authors":"H. Muruz, Enes Atmaca, A. Aksoy","doi":"10.33988/auvfd.933798","DOIUrl":"https://doi.org/10.33988/auvfd.933798","url":null,"abstract":"This study compared the cholesterol levels and shell pigments (protoporphyrin and biliverdin) of chicken (conventional and organic), quail, pheasant, and goose eggs. The material for the study was chicken (organic system - Lohmann Brown and conventional system - HyLine Brown) eggs, quail ( Coturnix coturnix japonica ), goose (local), and pheasant ( Phasianus colchicus ) eggs homogeneously selected with a subjective scoring. High-performance liquid chromatography with photodiode array detection was used to analyze the samples (HPLC-PDA). There were no significant differences in the yolk cholesterol content of eggs between species. Based on mg/g of yolk, different poultry species had comparable amounts of cholesterol. Quail eggshells contained significantly more protoporphyrin (81.92 M/g) than chicken (conventional-organic) and pheasant eggshells (P<0.01), but conventional chicken eggshells contained less protoporphyrin (10.73 M/g) than other species (P<0.01). Biliverdin was found only in the eggshells of quail (2.83 M/g) and pheasant (1.02 M/g) (P<0.01). It was observed that white shelled goose eggs had no detectable pigment. Research is required to elucidate the role of diet, age, stressor, strain, and housing systems on protoporphyrin and biliverdin pigment concentrations and cholesterol in table eggs and breeder eggs production.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69604945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Gregorc, Caio E C Domingues, H. Tutun, S. Sevin
: Bees are the major pollinators in natural ecosystems and in the agricultural production of several crops used for human consumption. However, they are exposed to multiple stressors that are causing a serious decline in their population. We highlight a major one among them, the Varroa destructor mite (Varroa) that causes severe impacts on the health of honey bee colonies, transmitting a variety of viruses that can affect the survival ability of individual bees and entire colonies. Diagnosis and mite control methods have been intensively studied in recent decades, with many studies in different areas of knowledge having been conducted. This overview summarizes these studies with a focus on colony defense systems, biological characteristics of the parasite Varroa, diagnostic methods used to establish the infestation level of colonies, and currently used control methods.
{"title":"What has been done in the fight against Varroa destructor: from the past to the present","authors":"A. Gregorc, Caio E C Domingues, H. Tutun, S. Sevin","doi":"10.33988/auvfd.1029296","DOIUrl":"https://doi.org/10.33988/auvfd.1029296","url":null,"abstract":": Bees are the major pollinators in natural ecosystems and in the agricultural production of several crops used for human consumption. However, they are exposed to multiple stressors that are causing a serious decline in their population. We highlight a major one among them, the Varroa destructor mite (Varroa) that causes severe impacts on the health of honey bee colonies, transmitting a variety of viruses that can affect the survival ability of individual bees and entire colonies. Diagnosis and mite control methods have been intensively studied in recent decades, with many studies in different areas of knowledge having been conducted. This overview summarizes these studies with a focus on colony defense systems, biological characteristics of the parasite Varroa, diagnostic methods used to establish the infestation level of colonies, and currently used control methods.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2022-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48496423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Maksimović, A. Maksimović, Ismar Lutvikadić, M. Rifatbegović
Little is known about the presence of methicillin-resistant Staphylococcus aureus (MRSA) in a canary bird (Serinus canarius domesticus). Although MRSA in a canary bird was previously reported, to the best of our knowledge, this is the first full description of the isolation and antibiotic resistance pattern of MRSA in this pet bird. A swab was taken from the nonhealing wound on the lateral thigh of a four-year-old, caged, housed alone, male canary bird. After the identification of Staphylococcus aureus, the antibiotic susceptibility profile of the isolate was obtained by the disk diffusion test. According to the resistance to Oxacillin and Cefoxitin, the isolate was identified as MRSA. The mecA gene was confirmed by PCR. The bird was treated by offering drinking water medicated with an injectable enrofloxacin formulation at 200 mg/L over 10 days period. Two weeks after therapy, intensive contraction of the wound was observed with a reduction in size. A week later, the complete epithelization of the wound defect was verified. In this study, we could not confirm the source of infection in a canary bird, but we believe that transmission was from wild birds when the cage was putting out in order to allow the bird to sunbathe or more likely via contact with the owner. The results of this study underline the necessity for further investigations on the epidemiological role of canary birds as potential reservoirs of MRSA.
{"title":"Methicillin-resistant Staphylococcus aureus isolated from nonhealing wound in a canary bird (Serinus canarius domesticus)","authors":"Z. Maksimović, A. Maksimović, Ismar Lutvikadić, M. Rifatbegović","doi":"10.33988/auvfd.837567","DOIUrl":"https://doi.org/10.33988/auvfd.837567","url":null,"abstract":"Little is known about the presence of methicillin-resistant Staphylococcus aureus (MRSA) in a canary bird (Serinus canarius domesticus). Although MRSA in a canary bird was previously reported, to the best of our knowledge, this is the first full description of the isolation and antibiotic resistance pattern of MRSA in this pet bird. A swab was taken from the nonhealing wound on the lateral thigh of a four-year-old, caged, housed alone, male canary bird. After the identification of Staphylococcus aureus, the antibiotic susceptibility profile of the isolate was obtained by the disk diffusion test. According to the resistance to Oxacillin and Cefoxitin, the isolate was identified as MRSA. The mecA gene was confirmed by PCR. The bird was treated by offering drinking water medicated with an injectable enrofloxacin formulation at 200 mg/L over 10 days period. Two weeks after therapy, intensive contraction of the wound was observed with a reduction in size. A week later, the complete epithelization of the wound defect was verified. In this study, we could not confirm the source of infection in a canary bird, but we believe that transmission was from wild birds when the cage was putting out in order to allow the bird to sunbathe or more likely via contact with the owner. The results of this study underline the necessity for further investigations on the epidemiological role of canary birds as potential reservoirs of MRSA.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69604194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ghasemian, M. Gholami-Ahangaran, Oveys Pourmahdi, A. Ahmadi-Dastgerdi
Application of phytobiotic and probiotic in poultry production can positive effect on performance and produce healthy protein source for human. In this study, the effects of one commercial probiotic and phytobiotic was evaluated on chicken performance. Totally, 300 chicks were divided into 4 groups that fed basal diet, diet containing probiotic (Protexin), phytobiotic (AE), and probiotic (Protexin) plus phytobiotic (AE) in all over the growing period. The growth indices were measured weekly and analyzed in 21 and 42 days of age. At the 42 days of age, blood samples were collected from all chickens. The concentration of liver enzymes, lipid profiles and antioxidant status were measured in blood samples. Results showed that the weight gain was significantly higher and FCR significantly lower in chickens received probiotic, or probiotic plus phytobiotic in comparison with chickens received phytobiotic and control chickens (p<0.05). Furthermore, addition of phytobiotic plus probiotic can significantly increase the activity of blood GPx and TAS with respect to chickens that received probiotic and phytobiotic, alonely. The TG, CHL, and LDL was lower and HDL was higher in chickens received phytobiotic or probiotic plus phytobiotic with the comparison of chickens fed probiotic and control chickens (p <0.05). The level of AST, ALT and ALP show significant decrease in chickens fed probiotic plus phytobiotic. In conclusion, continuous utilization of probiotic (Protexin) along with phytobiotic (Artichoke extract) in broiler diets can improve growth indices, increase antioxidant capacity, reduce serum lipids and improve liver function in broiler chickens.
{"title":"Dietary supplementation of protexin and artichoke extract for improving growth indices, lipid profile and antioxidant capacity in broilers chickens","authors":"S. Ghasemian, M. Gholami-Ahangaran, Oveys Pourmahdi, A. Ahmadi-Dastgerdi","doi":"10.33988/auvfd.833094","DOIUrl":"https://doi.org/10.33988/auvfd.833094","url":null,"abstract":"Application of phytobiotic and probiotic in poultry production can positive effect on performance and produce healthy protein source for human. In this study, the effects of one commercial probiotic and phytobiotic was evaluated on chicken performance. Totally, 300 chicks were divided into 4 groups that fed basal diet, diet containing probiotic (Protexin), phytobiotic (AE), and probiotic (Protexin) plus phytobiotic (AE) in all over the growing period. The growth indices were measured weekly and analyzed in 21 and 42 days of age. At the 42 days of age, blood samples were collected from all chickens. The concentration of liver enzymes, lipid profiles and antioxidant status were measured in blood samples. Results showed that the weight gain was significantly higher and FCR significantly lower in chickens received probiotic, or probiotic plus phytobiotic in comparison with chickens received phytobiotic and control chickens (p<0.05). Furthermore, addition of phytobiotic plus probiotic can significantly increase the activity of blood GPx and TAS with respect to chickens that received probiotic and phytobiotic, alonely. The TG, CHL, and LDL was lower and HDL was higher in chickens received phytobiotic or probiotic plus phytobiotic with the comparison of chickens fed probiotic and control chickens (p <0.05). The level of AST, ALT and ALP show significant decrease in chickens fed probiotic plus phytobiotic. In conclusion, continuous utilization of probiotic (Protexin) along with phytobiotic (Artichoke extract) in broiler diets can improve growth indices, increase antioxidant capacity, reduce serum lipids and improve liver function in broiler chickens.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69603354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meat yield and chemical composition of Potamon persicum Pretzmann, 1962","authors":"Ş. Karakaya, Pınar Karatepe, H. Yalçin, A. Arslan","doi":"10.33988/auvfd.1006294","DOIUrl":"https://doi.org/10.33988/auvfd.1006294","url":null,"abstract":"","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48559448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Newcastle Disease (ND) in Turkey Between 2017-2019 Using Geographical Information Systems","authors":"T. Bayir","doi":"10.33988/auvfd.959951","DOIUrl":"https://doi.org/10.33988/auvfd.959951","url":null,"abstract":"","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49384646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Kabakci, A. Yigit, H. Ekici, Y. Aluc, Erdal Kara
This study was conducted to investigate heavy metal concentrations and oxidative status of plasma and erythrocytes in Angora cats at different ages and gender. Sixteen young (less than 1 year old) and 14 adult (1–6 years old) cats were also grouped according to gender as male ( n = 17) and female ( n = 13). The separated plasma samples from cat’s blood were analyzed for selected heavy metals and total oxidant and antioxidant capacities (TOC and TAC) and calculated for oxidative stress index (OSI). The erythrocyte hemolysates were also evaluated for malondialdehyde (MDA), and super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Results of the study showed that most of the measured metals were not varied statistically according to age or gender. However, adult cats had significantly (P<0.01) higher Cu and lower Fe levels compared to young cats. Plasma levels of TOC, TAC and OSI, and erythrocyte MDA concentrations in young cats were significantly (P<0.05) higher than that of adults. While the SOD activity was decreased by the age, GPX activity was increased (P<0.05). However, the activity of CAT was changed by only gender, which was higher in males (P<0.01). In conclusion, metals, especially trace elements, are required for many kinds of physiological processes and the synthesis of antioxidant enzymes. Therefore, it can be suggested that the periodic measurement of metals and the addition of common antioxidant supplements to the diet of adult Angora cats will support weakening antioxidant mechanisms by age.
{"title":"Determination of blood heavy metal concentrations and oxidant-antioxidant capacities in Angora cats at different age and gender","authors":"R. Kabakci, A. Yigit, H. Ekici, Y. Aluc, Erdal Kara","doi":"10.33988/auvfd.931057","DOIUrl":"https://doi.org/10.33988/auvfd.931057","url":null,"abstract":"This study was conducted to investigate heavy metal concentrations and oxidative status of plasma and erythrocytes in Angora cats at different ages and gender. Sixteen young (less than 1 year old) and 14 adult (1–6 years old) cats were also grouped according to gender as male ( n = 17) and female ( n = 13). The separated plasma samples from cat’s blood were analyzed for selected heavy metals and total oxidant and antioxidant capacities (TOC and TAC) and calculated for oxidative stress index (OSI). The erythrocyte hemolysates were also evaluated for malondialdehyde (MDA), and super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Results of the study showed that most of the measured metals were not varied statistically according to age or gender. However, adult cats had significantly (P<0.01) higher Cu and lower Fe levels compared to young cats. Plasma levels of TOC, TAC and OSI, and erythrocyte MDA concentrations in young cats were significantly (P<0.05) higher than that of adults. While the SOD activity was decreased by the age, GPX activity was increased (P<0.05). However, the activity of CAT was changed by only gender, which was higher in males (P<0.01). In conclusion, metals, especially trace elements, are required for many kinds of physiological processes and the synthesis of antioxidant enzymes. Therefore, it can be suggested that the periodic measurement of metals and the addition of common antioxidant supplements to the diet of adult Angora cats will support weakening antioxidant mechanisms by age.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69604876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to demonstrate the incidence of deafness in Van cats and its distribution by eye color. A total of 300 Van cats aged between 2 months and 8 years were classified into three separate groups (equal in number) subjected to hearing tests using Clinical ABR (Auditory Brain Response) device. In this study, the incidence of deafness in Van cats was found to be 14.33%. Moreover, it was determined that deafness was not related to sex and hair length. Van cats with spots on their heads did not have deafness. The hearing thresholds showed that most Van cats had a very good level of hearing (0-10 dB nHL). The incidence of unilateral deafness was much lower in Van cats than it was in other white cat breeds. By means of this study, the breeding of the cats found to be deaf according to the results of ABR test will be prevented, and in this way we believe that the incidence of deafness in Van cats will decrease in future. This study is the first deafness study conducted in Van cats and it is worth to present as the findings of the study will shed light on future studies.
本研究的目的是证明耳聋的发生率和其眼睛颜色的分布。将300只年龄在2个月至8岁之间的Van猫分为三组(数量相等)，使用临床ABR(听觉脑反应)设备进行听力测试。本研究发现Van猫的耳聋发生率为14.33%。此外，还确定耳聋与性别和头发长度无关。头上有斑点的猫没有耳聋。听力阈值显示，大多数Van猫的听力水平非常好(0-10 dB nHL)。厢式猫单侧耳聋的发生率远低于其他品种的白猫。通过本研究，可以防止根据ABR测试结果发现的失聪猫的繁殖，我们相信未来Van猫的失聪发生率将会下降。这项研究是首次在范猫身上进行的耳聋研究，值得介绍，因为这项研究的结果将为未来的研究提供启示。
{"title":"Investigations on the incidence of deafness in Van cats and its distribution by eye color","authors":"Funda ALMAÇ ÇELİK, A. Kaya","doi":"10.33988/auvfd.975163","DOIUrl":"https://doi.org/10.33988/auvfd.975163","url":null,"abstract":"This study aims to demonstrate the incidence of deafness in Van cats and its distribution by eye color. A total of 300 Van cats aged between 2 months and 8 years were classified into three separate groups (equal in number) subjected to hearing tests using Clinical ABR (Auditory Brain Response) device. In this study, the incidence of deafness in Van cats was found to be 14.33%. Moreover, it was determined that deafness was not related to sex and hair length. Van cats with spots on their heads did not have deafness. The hearing thresholds showed that most Van cats had a very good level of hearing (0-10 dB nHL). The incidence of unilateral deafness was much lower in Van cats than it was in other white cat breeds. By means of this study, the breeding of the cats found to be deaf according to the results of ABR test will be prevented, and in this way we believe that the incidence of deafness in Van cats will decrease in future. This study is the first deafness study conducted in Van cats and it is worth to present as the findings of the study will shed light on future studies.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69605635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Timurkaan, H. Öngör, H. Kalender, B. Karabulut, F. ÇÖven, A. Çevik, H. Eröksüz, B. Çetinkaya
The aim of this study was to investigate pathological lesions and the presence of of Israel variant 2 (IS /1494/06) genotype of infectious bronchitis virus (IBV) in chickens with visceral gout. Sudden deaths were observed in broiler breeders and layer hens belonging to two different flocks located in eastern Türkiye. Broiler chickens were previously vaccinated with a vaccine containing strains of IBV Massachusetts serotype, but no information was available about the vaccination history of laying hens. At necropsy, there was accumulation of white chalk-like material on the serosal surface of the heart, liver, spleen and air sacs. The kidneys were unilaterally or bilaterally enlarged and pale in color and, ureters were also enlarged. Pooled organ samples from diseased chickens and kidneys alone were examined by virus isolation, real-time reverse transcription polymerase chain reaction (rRT-PCR), nucleotide sequencing analysis and histological methods. Israel variant 2 genotype of IBV was detected in the samples of both flocks as a result of virus isolation, rRT-PCR and DNA sequencing analysis. Histological examination revealed multifocal and randomly distributed crystal deposits in the renal tubulus and adjacent interstitium. Mild to moderate crystalline deposits surrounded by heterophils and macrophages were detected in the serosal layers of the heart, spleen, liver, and air sacs. The findings of this study indicated that IBV should be taken into consideration in visceral gout cases of chickens, and detection of IBV genotypes in the field will enable us to use vaccines compatible with these genotypes in order to control the disease more efficiently.
{"title":"Pathological and molecular findings of visceral gout caused by Israel variant 2 (IS/1494/06) genotype of infectious bronchitis virus in chickens","authors":"N. Timurkaan, H. Öngör, H. Kalender, B. Karabulut, F. ÇÖven, A. Çevik, H. Eröksüz, B. Çetinkaya","doi":"10.33988/auvfd.974221","DOIUrl":"https://doi.org/10.33988/auvfd.974221","url":null,"abstract":"The aim of this study was to investigate pathological lesions and the presence of of Israel variant 2 (IS /1494/06) genotype of infectious bronchitis virus (IBV) in chickens with visceral gout. Sudden deaths were observed in broiler breeders and layer hens belonging to two different flocks located in eastern Türkiye. Broiler chickens were previously vaccinated with a vaccine containing strains of IBV Massachusetts serotype, but no information was available about the vaccination history of laying hens. At necropsy, there was accumulation of white chalk-like material on the serosal surface of the heart, liver, spleen and air sacs. The kidneys were unilaterally or bilaterally enlarged and pale in color and, ureters were also enlarged. Pooled organ samples from diseased chickens and kidneys alone were examined by virus isolation, real-time reverse transcription polymerase chain reaction (rRT-PCR), nucleotide sequencing analysis and histological methods. Israel variant 2 genotype of IBV was detected in the samples of both flocks as a result of virus isolation, rRT-PCR and DNA sequencing analysis. Histological examination revealed multifocal and randomly distributed crystal deposits in the renal tubulus and adjacent interstitium. Mild to moderate crystalline deposits surrounded by heterophils and macrophages were detected in the serosal layers of the heart, spleen, liver, and air sacs. The findings of this study indicated that IBV should be taken into consideration in visceral gout cases of chickens, and detection of IBV genotypes in the field will enable us to use vaccines compatible with these genotypes in order to control the disease more efficiently.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69605185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：