The aim of the study is to investigate the effects of humic acid and boric acid on rumen fermentation parameters in rams. For this purpose, 3 rams were used in this experiment. Rams in each treatment; a control with no supplements (C), 180 ppm boric acid (B) and 5 ml/kg humic acid (H) with 65:35 forage to concentrate ratio. Each experimental period lasted 14 days, with 12 first days of diet adaptation. Totally the experimental period lasted 56 days. In both time periods (0 and 3h after feeding), there were no significant difference (P>0.05) on rumen pH, ammonia, protozoa count, estimated methane production and volatile fatty acid composition. However, butyric acid concentration tended to be higher in experimental groups (P=0.08). As a result of this study, these feed additives did not modify the rumen milieu and showed no negative effect in rams.
{"title":"Rumen fermentation characteristics of rams fed supplemental boric acid and humic acid diets","authors":"Ö. Sızmaz, B. H. Köksal, G. Yıldız","doi":"10.33988/auvfd.1059346","DOIUrl":"https://doi.org/10.33988/auvfd.1059346","url":null,"abstract":"The aim of the study is to investigate the effects of humic acid and boric acid on rumen fermentation parameters in rams. For this purpose, 3 rams were used in this experiment. Rams in each treatment; a control with no supplements (C), 180 ppm boric acid (B) and 5 ml/kg humic acid (H) with 65:35 forage to concentrate ratio. Each experimental period lasted 14 days, with 12 first days of diet adaptation. Totally the experimental period lasted 56 days. In both time periods (0 and 3h after feeding), there were no significant difference (P>0.05) on rumen pH, ammonia, protozoa count, estimated methane production and volatile fatty acid composition. However, butyric acid concentration tended to be higher in experimental groups (P=0.08). As a result of this study, these feed additives did not modify the rumen milieu and showed no negative effect in rams.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48052246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazlı Türkmen, B. Baş, Rabia Albayrak Delialioğlu, A. Gürsoy
In this study
在这项研究中
{"title":"Physical properties and bacterial viability of functional ice cream enriched with kefir","authors":"Nazlı Türkmen, B. Baş, Rabia Albayrak Delialioğlu, A. Gürsoy","doi":"10.33988/auvfd.1008552","DOIUrl":"https://doi.org/10.33988/auvfd.1008552","url":null,"abstract":"In this study","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69592213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Research in recent years has focused on innovative technologies that provide pathogen inactivation without damaging the structural properties of foods. Ohmic heating (OH) is an innovative technology, that provides an effective microbial inactivation with massive and rapid heating. This study aims to determine the effects of milk fat on the inactivation of Listeria monocytogenes by OH with a low voltage gradient. L. monocytogenes (ATCC 13932) inoculated 3.1%, 1.5%, and 0.1% fat-milk samples were heated up to 62°C by OH and conventional heating (CH) process. OH treatment lead to the inactivation of L. monocytogenes in both 1.5% and 0.1% groups and led to approximately 5.30 log decrease, however, there was a 3.10 log decrease in the 3.1% group at 6 min. CH lead to a few reduction as 0.21, 0.29 and 0.39 log in 3.1%, 1.5% and 0.1% fat-milk respectively. In OH, the sublethal injury ratio was higher than CH in all milk groups. However, OH did not statistically change color and pH values at the 6 th min of the process, had a significant effect on hydroxymethylfurfural value only in 3.1% fat-milk. In conclusion, the increased fat content may have important inhibitory effects on pathogen inactivation in OH. Thus, the OH conditions should be chosen carefully to sufficient inactivation of pathogens in milk with high-fat content.
{"title":"Determination of the Effect of Milk Fat on the Inactivation of Listeria monocytogenes by Ohmic Heating","authors":"Serap Özkale, H. Kahraman","doi":"10.33988/auvfd.1069886","DOIUrl":"https://doi.org/10.33988/auvfd.1069886","url":null,"abstract":"Research in recent years has focused on innovative technologies that provide pathogen inactivation without damaging the structural properties of foods. Ohmic heating (OH) is an innovative technology, that provides an effective microbial inactivation with massive and rapid heating. This study aims to determine the effects of milk fat on the inactivation of Listeria monocytogenes by OH with a low voltage gradient. L. monocytogenes (ATCC 13932) inoculated 3.1%, 1.5%, and 0.1% fat-milk samples were heated up to 62°C by OH and conventional heating (CH) process. OH treatment lead to the inactivation of L. monocytogenes in both 1.5% and 0.1% groups and led to approximately 5.30 log decrease, however, there was a 3.10 log decrease in the 3.1% group at 6 min. CH lead to a few reduction as 0.21, 0.29 and 0.39 log in 3.1%, 1.5% and 0.1% fat-milk respectively. In OH, the sublethal injury ratio was higher than CH in all milk groups. However, OH did not statistically change color and pH values at the 6 th min of the process, had a significant effect on hydroxymethylfurfural value only in 3.1% fat-milk. In conclusion, the increased fat content may have important inhibitory effects on pathogen inactivation in OH. Thus, the OH conditions should be chosen carefully to sufficient inactivation of pathogens in milk with high-fat content.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69595388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Grković, I. ZUBER BOGDANOVİC, S. Djuric, N. Karabasil, B. Suvajdžić, N. Čobanović, V. Djordjević, D. Vasilev, M. Dimitrijević
Seasonal variation occurring in all seasons in one year in the nutrient composition of Mytilus galloprovincialis, cultivated in Boka Kotorska Bay, Montenegro, was investigated in this study. Biometric parameters, meat yield, condition index, proximate composition, minerals, as well as the lipid and fatty acid compositions of mussels were analyzed. Most significant factors determining these parameters were temperature, food amount and gametogenesis The biometric parameters showed no significant differences during the sampling period. In the spring, meat yields and mussel condition index increased at significant levels. Low values od meat yield and condition index during cold months has been a result of food shortage and reproductive cycle, when mussels use carbohydrates and protein reserves. The highest amount of protein was detected in mussels harvested in August (10.76%), while the highest amount of lipids was recorded in the winter months (2.11%). Fatty acid profiles revealed the predominance of polyunsaturated fatty acids (PUFA), followed by saturated (SFAs) and monounsaturated (MUFAs) fatty acids. Docosahexaenoic acid and eicosapentaenoic acid were the most abundant PUFA. The concentration of metals found in mussels from the study area is within the range of mean values reported in the literature. Our results indicate that the best period for mussels harvesting were during the spring and summer (April and August), opposite the winter months when the mussels were not favorable for harvesting.
{"title":"Seasonal variation in the nutrient composition of mussels (Mytilus galloprovincialis) from farms in Boka Kotorska Bay, Southern Adriatic Sea","authors":"N. Grković, I. ZUBER BOGDANOVİC, S. Djuric, N. Karabasil, B. Suvajdžić, N. Čobanović, V. Djordjević, D. Vasilev, M. Dimitrijević","doi":"10.33988/auvfd.920577","DOIUrl":"https://doi.org/10.33988/auvfd.920577","url":null,"abstract":"Seasonal variation occurring in all seasons in one year in the nutrient composition of Mytilus galloprovincialis, cultivated in Boka Kotorska Bay, Montenegro, was investigated in this study. Biometric parameters, meat yield, condition index, proximate composition, minerals, as well as the lipid and fatty acid compositions of mussels were analyzed. Most significant factors determining these parameters were temperature, food amount and gametogenesis The biometric parameters showed no significant differences during the sampling period. In the spring, meat yields and mussel condition index increased at significant levels. Low values od meat yield and condition index during cold months has been a result of food shortage and reproductive cycle, when mussels use carbohydrates and protein reserves. The highest amount of protein was detected in mussels harvested in August (10.76%), while the highest amount of lipids was recorded in the winter months (2.11%). Fatty acid profiles revealed the predominance of polyunsaturated fatty acids (PUFA), followed by saturated (SFAs) and monounsaturated (MUFAs) fatty acids. Docosahexaenoic acid and eicosapentaenoic acid were the most abundant PUFA. The concentration of metals found in mussels from the study area is within the range of mean values reported in the literature. Our results indicate that the best period for mussels harvesting were during the spring and summer (April and August), opposite the winter months when the mussels were not favorable for harvesting.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69604773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The diplectanid monogenean Diplectanum aequans ( D. aequans ) (Wegener, 1857) is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax ( Dic. labrax ) (Linnaeus, 1758) worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea (FAO area 37.4.2) and Aegean Sea (FAO area 37.1.3) coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100% and 15.1, respectively. This study reports first information of molecular (28S and 18S rRNA) evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.
{"title":"An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (L.) from Turkish coasts","authors":"C. Aydın, G. Z. Pekmezci","doi":"10.33988/auvfd.987702","DOIUrl":"https://doi.org/10.33988/auvfd.987702","url":null,"abstract":"The diplectanid monogenean Diplectanum aequans ( D. aequans ) (Wegener, 1857) is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax ( Dic. labrax ) (Linnaeus, 1758) worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea (FAO area 37.4.2) and Aegean Sea (FAO area 37.1.3) coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100% and 15.1, respectively. This study reports first information of molecular (28S and 18S rRNA) evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48512880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Case of Cheilognathopalatoschisis in a Simmental Breed Calf: A Rare Case","authors":"A. Durmuş, E. Polat","doi":"10.33988/auvfd.951261","DOIUrl":"https://doi.org/10.33988/auvfd.951261","url":null,"abstract":"","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69605024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Artificial insemination in a cat: Report of first successful performance resulted with parturition in Turkey","authors":"A. Daskin, Esra Totan, K. T. Olğaç","doi":"10.33988/auvfd.985216","DOIUrl":"https://doi.org/10.33988/auvfd.985216","url":null,"abstract":"","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69605272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nursel Dikmen, H. Özkan, Funda Cimen, B. Camdeviren, E. Ay, P. Ambarcioglu, N. Duran, Akın Yakin
The aim of study was to investigate the anti-proliferative and inflammatory effects of atorvastatin, rosuvastatin, and simvastatin in lung cancer. The effects of statins were investigated in Vero, BEAS-2B, and A549 cell lines. In addition to expressions of BAX, BCL-2, TNFα, IL-10, IL-6 , protein levels of TNFα, IL-10, IL-6 were determined. Cell viability and MDA were also measured. While the cell numbers in groups with low doses of statins were found to be approximately 1x10 6 /mL, proliferation was inhibited at higher rates containing high doses. Simvastatin, rosuvastatin, and high dose atorvastatin upregulated the BAX , while high dose of atorvastatin and both doses of rosuvastatin caused downregulation in BCL-2 . All statin groups had higher MDA. Simvastatin and high dose rosuvastatin upregulated TNFα . While low dose simvastatin and atorvastatin and high dose atorvastatin and rosuvastatin upregulated IL-10 , IL-6 was upregulated with a low dose of rosuvastatin. TNFα was higher in simvastatin and rosuvastatin groups. IL-10 was highest in rosuvastatin groups. Atorvastatin groups had lower IL-6. Although cell numbers have been reduced by all statins, rosuvastatin is more effective on studied genes.
{"title":"Dose-dependent effects of simvastatin, atorvastatin and rosuvastatin on apoptosis and inflammation pathways on cancerous lung cells","authors":"Nursel Dikmen, H. Özkan, Funda Cimen, B. Camdeviren, E. Ay, P. Ambarcioglu, N. Duran, Akın Yakin","doi":"10.33988/auvfd.938418","DOIUrl":"https://doi.org/10.33988/auvfd.938418","url":null,"abstract":"The aim of study was to investigate the anti-proliferative and inflammatory effects of atorvastatin, rosuvastatin, and simvastatin in lung cancer. The effects of statins were investigated in Vero, BEAS-2B, and A549 cell lines. In addition to expressions of BAX, BCL-2, TNFα, IL-10, IL-6 , protein levels of TNFα, IL-10, IL-6 were determined. Cell viability and MDA were also measured. While the cell numbers in groups with low doses of statins were found to be approximately 1x10 6 /mL, proliferation was inhibited at higher rates containing high doses. Simvastatin, rosuvastatin, and high dose atorvastatin upregulated the BAX , while high dose of atorvastatin and both doses of rosuvastatin caused downregulation in BCL-2 . All statin groups had higher MDA. Simvastatin and high dose rosuvastatin upregulated TNFα . While low dose simvastatin and atorvastatin and high dose atorvastatin and rosuvastatin upregulated IL-10 , IL-6 was upregulated with a low dose of rosuvastatin. TNFα was higher in simvastatin and rosuvastatin groups. IL-10 was highest in rosuvastatin groups. Atorvastatin groups had lower IL-6. Although cell numbers have been reduced by all statins, rosuvastatin is more effective on studied genes.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69605009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, it was aimed to investigate the effect of a poly(ethylene terephthalate)-g-poly(hydroxyethyl methacrylate) (PET-g-HEMA) nanofiber wound dressing modified with sericin-coated silver nanoparticles (S-AgNPs) on internal organs, oxidative stress, and biochemical parameters. To establish a burn model, the backs of anesthetized rats were shaved and then third-degree burns were created with a round-bottomed stainless steel rod 2 cm in diameter kept in 100 °C water for 20 seconds. The wounds of the negative control group (G1) were covered with standard bandages; the wounds of the positive control group (G2) were covered with silvercel, used as burn wound material; and the wounds of the experimental group (G3) were covered with PET-based dressing material. Histopathological changes in organs (liver, kidneys, heart, pancreas, lungs), total oxidant status (TOS), total antioxidant status (TAS), nitric oxide (NO), and biochemical parameters (serum aspartate aminotransferase [AST], alanine aminotransferase [ALT], gamma glutamyl transpeptidase [GGT], creatine kinase, lactate dehydrogenase [LDH], total protein, albumin, globulin, urea) were examined. Compared with the G1 group, plasma AST, ALT, and GGT levels were found to be significantly decreased in G2 and G3 (P<0.001). Plasma TAS was found to be significantly increased in G2 and G3 compared to G1 (P<0.05). Compared to the G1 group, degenerative and necrotic changes in the liver, kidneys, and pancreas were found to be significantly reduced in G2 and G3 (P<0.05). In conclusion, this work demonstrates that the synthesized PET-based wound dressing material has the capacity to be used commercially.
{"title":"Effects of functional poly(ethylene terephthalate) nanofibers modified with sericin-capped silver nanoparticles on histopathological changes in parenchymal organs and oxidative stress in a rat burn wound model","authors":"Y. Şahin, Zehra Gün Gök, M. E. Alçığır, M. Çınar","doi":"10.33988/auvfd.990270","DOIUrl":"https://doi.org/10.33988/auvfd.990270","url":null,"abstract":"In this study, it was aimed to investigate the effect of a poly(ethylene terephthalate)-g-poly(hydroxyethyl methacrylate) (PET-g-HEMA) nanofiber wound dressing modified with sericin-coated silver nanoparticles (S-AgNPs) on internal organs, oxidative stress, and biochemical parameters. To establish a burn model, the backs of anesthetized rats were shaved and then third-degree burns were created with a round-bottomed stainless steel rod 2 cm in diameter kept in 100 °C water for 20 seconds. The wounds of the negative control group (G1) were covered with standard bandages; the wounds of the positive control group (G2) were covered with silvercel, used as burn wound material; and the wounds of the experimental group (G3) were covered with PET-based dressing material. Histopathological changes in organs (liver, kidneys, heart, pancreas, lungs), total oxidant status (TOS), total antioxidant status (TAS), nitric oxide (NO), and biochemical parameters (serum aspartate aminotransferase [AST], alanine aminotransferase [ALT], gamma glutamyl transpeptidase [GGT], creatine kinase, lactate dehydrogenase [LDH], total protein, albumin, globulin, urea) were examined. Compared with the G1 group, plasma AST, ALT, and GGT levels were found to be significantly decreased in G2 and G3 (P<0.001). Plasma TAS was found to be significantly increased in G2 and G3 compared to G1 (P<0.05). Compared to the G1 group, degenerative and necrotic changes in the liver, kidneys, and pancreas were found to be significantly reduced in G2 and G3 (P<0.05). In conclusion, this work demonstrates that the synthesized PET-based wound dressing material has the capacity to be used commercially.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48296469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}