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Rumen fermentation characteristics of rams fed supplemental boric acid and humic acid diets 添加硼酸和腐殖酸日粮的公羊瘤胃发酵特性
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-03-23 DOI: 10.33988/auvfd.1059346
Ö. Sızmaz, B. H. Köksal, G. Yıldız
The aim of the study is to investigate the effects of humic acid and boric acid on rumen fermentation parameters in rams. For this purpose, 3 rams were used in this experiment. Rams in each treatment; a control with no supplements (C), 180 ppm boric acid (B) and 5 ml/kg humic acid (H) with 65:35 forage to concentrate ratio. Each experimental period lasted 14 days, with 12 first days of diet adaptation. Totally the experimental period lasted 56 days. In both time periods (0 and 3h after feeding), there were no significant difference (P>0.05) on rumen pH, ammonia, protozoa count, estimated methane production and volatile fatty acid composition. However, butyric acid concentration tended to be higher in experimental groups (P=0.08). As a result of this study, these feed additives did not modify the rumen milieu and showed no negative effect in rams.
本试验旨在探讨腐植酸和硼酸对公羊瘤胃发酵参数的影响。为此,本试验选用公羊3只。每组为公羊;对照组不添加任何添加剂(C),硼酸180 ppm (B)和腐植酸5 ml/kg (H),料精比65:35。每期试验期14 d,预试期12 d。试验期56 d。在饲喂后0和3h,瘤胃pH、氨、原生动物数量、估算甲烷产量和挥发性脂肪酸组成均无显著差异(P < 0.05)。但各试验组丁酸浓度有升高趋势(P=0.08)。本研究结果表明,这些饲料添加剂没有改变瘤胃环境,对公羊没有负面影响。
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引用次数: 2
Physical properties and bacterial viability of functional ice cream enriched with kefir 加了克菲尔的功能性冰淇淋的物理特性和细菌活力
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-03-21 DOI: 10.33988/auvfd.1008552
Nazlı Türkmen, B. Baş, Rabia Albayrak Delialioğlu, A. Gürsoy
In this study
在这项研究中
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引用次数: 0
Determination of the Effect of Milk Fat on the Inactivation of Listeria monocytogenes by Ohmic Heating 欧姆加热法测定乳脂对单核增生李斯特菌灭活的影响
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-03-21 DOI: 10.33988/auvfd.1069886
Serap Özkale, H. Kahraman
Research in recent years has focused on innovative technologies that provide pathogen inactivation without damaging the structural properties of foods. Ohmic heating (OH) is an innovative technology, that provides an effective microbial inactivation with massive and rapid heating. This study aims to determine the effects of milk fat on the inactivation of Listeria monocytogenes by OH with a low voltage gradient. L. monocytogenes (ATCC 13932) inoculated 3.1%, 1.5%, and 0.1% fat-milk samples were heated up to 62°C by OH and conventional heating (CH) process. OH treatment lead to the inactivation of L. monocytogenes in both 1.5% and 0.1% groups and led to approximately 5.30 log decrease, however, there was a 3.10 log decrease in the 3.1% group at 6 min. CH lead to a few reduction as 0.21, 0.29 and 0.39 log in 3.1%, 1.5% and 0.1% fat-milk respectively. In OH, the sublethal injury ratio was higher than CH in all milk groups. However, OH did not statistically change color and pH values at the 6 th min of the process, had a significant effect on hydroxymethylfurfural value only in 3.1% fat-milk. In conclusion, the increased fat content may have important inhibitory effects on pathogen inactivation in OH. Thus, the OH conditions should be chosen carefully to sufficient inactivation of pathogens in milk with high-fat content.
近年来的研究重点是在不破坏食品结构特性的情况下提供病原体灭活的创新技术。欧姆加热(OH)是一项创新技术,通过大量快速加热提供有效的微生物灭活。本研究旨在确定乳脂对低电压梯度OH对单核增生李斯特菌灭活的影响。分别接种3.1%、1.5%和0.1%脂乳样品的L. monocytogenes (ATCC 13932)通过OH和常规加热(CH)工艺加热至62℃。OH处理导致1.5%和0.1%组单核增生乳杆菌失活,6 min时减少5.30 log, 3.1%组减少3.10 log。CH在3.1%、1.5%和0.1%脂乳中分别减少0.21、0.29和0.39 log。在OH组,亚致死损伤率均高于CH组。然而,OH在第6分钟对颜色和pH值没有统计学意义上的改变,仅在3.1%的脂肪牛奶中对羟甲基糠醛值有显著影响。综上所述,脂肪含量的增加可能对OH中病原体的灭活具有重要的抑制作用。因此,应仔细选择OH条件,以充分灭活高脂肪含量牛奶中的病原体。
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引用次数: 0
Seasonal variation in the nutrient composition of mussels (Mytilus galloprovincialis)  from farms in Boka Kotorska Bay, Southern Adriatic Sea 南亚得里亚海Boka Kotorska湾养殖场贻贝(Mytilus galloprovincialis)营养成分的季节性变化
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-02-28 DOI: 10.33988/auvfd.920577
N. Grković, I. ZUBER BOGDANOVİC, S. Djuric, N. Karabasil, B. Suvajdžić, N. Čobanović, V. Djordjević, D. Vasilev, M. Dimitrijević
Seasonal variation occurring in all seasons in one year in the nutrient composition of Mytilus galloprovincialis, cultivated in Boka Kotorska Bay, Montenegro, was investigated in this study. Biometric parameters, meat yield, condition index, proximate composition, minerals, as well as the lipid and fatty acid compositions of mussels were analyzed. Most significant factors determining these parameters were temperature, food amount and gametogenesis The biometric parameters showed no significant differences during the sampling period. In the spring, meat yields and mussel condition index increased at significant levels. Low values od meat yield and condition index during cold months has been a result of food shortage and reproductive cycle, when mussels use carbohydrates and protein reserves. The highest amount of protein was detected in mussels harvested in August (10.76%), while the highest amount of lipids was recorded in the winter months (2.11%). Fatty acid profiles revealed the predominance of polyunsaturated fatty acids (PUFA), followed by saturated (SFAs) and monounsaturated (MUFAs) fatty acids. Docosahexaenoic acid and eicosapentaenoic acid were the most abundant PUFA. The concentration of metals found in mussels from the study area is within the range of mean values reported in the literature. Our results indicate that the best period for mussels harvesting were during the spring and summer (April and August), opposite the winter months when the mussels were not favorable for harvesting.
本研究调查了在黑山博卡科托斯卡湾栽培的加洛省贻贝(Mytilus galloprovincialis)一年中所有季节营养成分的季节变化。对贻贝的生物特征参数、产肉量、状态指数、近似组成、矿物质、脂质和脂肪酸组成进行了分析。温度、食物量和配子体发育是影响这些参数的最重要因素。在春季,肉产量和贻贝状态指数均显著提高。在寒冷的月份,低的鲜肉产量和状态指数是食物短缺和繁殖周期的结果,贻贝利用碳水化合物和蛋白质储备。8月份捕获的贻贝蛋白质含量最高(10.76%),而脂质含量最高(2.11%)。脂肪酸谱显示多不饱和脂肪酸(PUFA)占主导地位,其次是饱和脂肪酸(sfa)和单不饱和脂肪酸(MUFAs)。二十二碳六烯酸和二十碳五烯酸是最丰富的PUFA。在研究区域的贻贝中发现的金属浓度在文献报道的平均值范围内。结果表明,春季和夏季(4月和8月)是贻贝的最佳采收季节,冬季则是贻贝的不利采收季节。
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引用次数: 0
An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (L.) from Turkish coasts 土耳其海岸欧洲黑鲈感染水双斑绦虫(双斑绦虫科:单属)形态和分子特征的综合研究
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-02-15 DOI: 10.33988/auvfd.987702
C. Aydın, G. Z. Pekmezci
The diplectanid monogenean Diplectanum aequans ( D. aequans ) (Wegener, 1857) is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax ( Dic. labrax ) (Linnaeus, 1758) worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea (FAO area 37.4.2) and Aegean Sea (FAO area 37.1.3) coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100% and 15.1, respectively. This study reports first information of molecular (28S and 18S rRNA) evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.
双尾目单尾目双尾目(D.aequans)(Wegener,1857)是世界各地野生和养殖的欧洲鲈鱼Dicentrarchus labrax(Dic.labrax)(Linnaeus,1758)中最重要的体外寄生虫之一。目前,还没有关于土耳其海岸的D.aequans分子数据的信息。在本研究中,从野生和培养的Dic中采集了双锥虫和单烯虫。土耳其黑海(粮农组织区域37.4.2)和爱琴海(粮农组织地区37.1.3)海岸的labrax,通过28S rRNA和18S rRNA片段测序进行形态鉴定和基因特征鉴定。根据部分28S rRNA和18S rRNA基因的详细形态特征和序列特征,收集到的二聚体被明确鉴定为D.aequans。伊豆的总患病率和平均强度分别为100%和15.1。本研究报道了来自Dic的D.aequans的分子(28S和18S rRNA)证据的首次信息。土耳其海岸的labrax。对从黑海和爱琴海分离得到的伊豆新的18S和28S rRNA序列进行了遗传学表征。阿氏锥虫的28S和18S rRNA序列可用于解析在黑海和地中海的双锥虫科中发现的物种的系统发育位置。
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引用次数: 1
A Case of Cheilognathopalatoschisis in a Simmental Breed Calf: A Rare Case 西门塔尔种小牛的切颌腭裂一例:罕见病例
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-02-10 DOI: 10.33988/auvfd.951261
A. Durmuş, E. Polat
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引用次数: 0
Artificial insemination in a cat: Report of first successful performance resulted with parturition in Turkey 人工授精在猫:报告首次成功的表现导致在土耳其分娩
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-02-02 DOI: 10.33988/auvfd.985216
A. Daskin, Esra Totan, K. T. Olğaç
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引用次数: 1
An Evaluation of Turkey Livestock Congresses (1968-2000) 土耳其畜牧大会评价(1968-2000)
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-02-02 DOI: 10.33988/auvfd.1009718
A. Yiğit, Şule Sanal, Ayşe MENTEŞ GÜRLER
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引用次数: 0
Dose-dependent effects of simvastatin, atorvastatin and rosuvastatin on apoptosis and inflammation pathways on cancerous lung cells 辛伐他汀、阿托伐他汀和瑞舒伐他汀对肺癌细胞凋亡和炎症通路的剂量依赖性影响
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-01-27 DOI: 10.33988/auvfd.938418
Nursel Dikmen, H. Özkan, Funda Cimen, B. Camdeviren, E. Ay, P. Ambarcioglu, N. Duran, Akın Yakin
The aim of study was to investigate the anti-proliferative and inflammatory effects of atorvastatin, rosuvastatin, and simvastatin in lung cancer. The effects of statins were investigated in Vero, BEAS-2B, and A549 cell lines. In addition to expressions of BAX, BCL-2, TNFα, IL-10, IL-6 , protein levels of TNFα, IL-10, IL-6 were determined. Cell viability and MDA were also measured. While the cell numbers in groups with low doses of statins were found to be approximately 1x10 6 /mL, proliferation was inhibited at higher rates containing high doses. Simvastatin, rosuvastatin, and high dose atorvastatin upregulated the BAX , while high dose of atorvastatin and both doses of rosuvastatin caused downregulation in BCL-2 . All statin groups had higher MDA. Simvastatin and high dose rosuvastatin upregulated TNFα . While low dose simvastatin and atorvastatin and high dose atorvastatin and rosuvastatin upregulated IL-10 , IL-6 was upregulated with a low dose of rosuvastatin. TNFα was higher in simvastatin and rosuvastatin groups. IL-10 was highest in rosuvastatin groups. Atorvastatin groups had lower IL-6. Although cell numbers have been reduced by all statins, rosuvastatin is more effective on studied genes.
本研究的目的是探讨阿托伐他汀、瑞舒伐他汀和辛伐他汀在肺癌中的抗增殖和炎症作用。研究了他汀类药物在Vero、BEAS-2B和A549细胞系中的作用。除BAX、BCL-2、TNFα、IL-10、IL-6表达外,检测TNFα、IL-10、IL-6蛋白表达水平。同时测定细胞活力和MDA。低剂量他汀类药物组的细胞数量约为1 × 10 6 /mL,高剂量他汀类药物组的增殖抑制率更高。辛伐他汀、瑞舒伐他汀和高剂量阿托伐他汀可上调BAX,而高剂量阿托伐他汀和两种剂量瑞舒伐他汀均可下调BCL-2。所有他汀类药物组均有较高的MDA。辛伐他汀和大剂量瑞舒伐他汀上调TNFα。低剂量辛伐他汀和阿托伐他汀以及高剂量阿托伐他汀和瑞舒伐他汀上调IL-10,而低剂量瑞舒伐他汀上调IL-6。辛伐他汀组和瑞舒伐他汀组TNFα升高。瑞舒伐他汀组IL-10最高。阿托伐他汀组IL-6较低。虽然所有他汀类药物都能减少细胞数量,但瑞舒伐他汀对所研究的基因更有效。
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引用次数: 0
Effects of functional poly(ethylene terephthalate) nanofibers modified with sericin-capped silver nanoparticles on histopathological changes in parenchymal organs and oxidative stress in a rat burn wound model 丝胶包覆银纳米粒子修饰功能性聚对苯二甲酸乙二醇酯纳米纤维对大鼠烧伤模型实质器官组织病理学变化和氧化应激的影响
IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2022-01-25 DOI: 10.33988/auvfd.990270
Y. Şahin, Zehra Gün Gök, M. E. Alçığır, M. Çınar
In this study, it was aimed to investigate the effect of a poly(ethylene terephthalate)-g-poly(hydroxyethyl methacrylate) (PET-g-HEMA) nanofiber wound dressing modified with sericin-coated silver nanoparticles (S-AgNPs) on internal organs, oxidative stress, and biochemical parameters. To establish a burn model, the backs of anesthetized rats were shaved and then third-degree burns were created with a round-bottomed stainless steel rod 2 cm in diameter kept in 100 °C water for 20 seconds. The wounds of the negative control group (G1) were covered with standard bandages; the wounds of the positive control group (G2) were covered with silvercel, used as burn wound material; and the wounds of the experimental group (G3) were covered with PET-based dressing material. Histopathological changes in organs (liver, kidneys, heart, pancreas, lungs), total oxidant status (TOS), total antioxidant status (TAS), nitric oxide (NO), and biochemical parameters (serum aspartate aminotransferase [AST], alanine aminotransferase [ALT], gamma glutamyl transpeptidase [GGT], creatine kinase, lactate dehydrogenase [LDH], total protein, albumin, globulin, urea) were examined. Compared with the G1 group, plasma AST, ALT, and GGT levels were found to be significantly decreased in G2 and G3 (P<0.001). Plasma TAS was found to be significantly increased in G2 and G3 compared to G1 (P<0.05). Compared to the G1 group, degenerative and necrotic changes in the liver, kidneys, and pancreas were found to be significantly reduced in G2 and G3 (P<0.05). In conclusion, this work demonstrates that the synthesized PET-based wound dressing material has the capacity to be used commercially.
本研究旨在探讨丝胶包覆银纳米粒子(S-AgNPs)修饰的聚对苯二甲酸乙酯-聚甲基丙烯酸羟乙酯(PET-g-HEMA)纳米纤维创面敷料对内脏器官、氧化应激和生化参数的影响。将麻醉后的大鼠背部剃光,用直径2 cm的圆底不锈钢棒在100℃水中放置20秒造成三度烧伤,建立烧伤模型。阴性对照组(G1)创面用标准绷带包扎;阳性对照组(G2)创面覆盖银细胞,作为烧伤创面材料;实验组(G3)创面覆盖pet基敷料。检测各脏器(肝、肾、心、胰、肺)的组织病理学变化、总氧化状态(TOS)、总抗氧化状态(TAS)、一氧化氮(NO)及生化指标(血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、谷氨酰转肽酶(GGT)、肌酸激酶、乳酸脱氢酶(LDH)、总蛋白、白蛋白、球蛋白、尿素)。与G1组比较,G2、G3组血浆AST、ALT、GGT水平均显著降低(P<0.001)。血浆TAS在G2、G3组较G1组明显升高(P<0.05)。与G1组比较,G2组和G3组肝、肾、胰腺的退行性和坏死性病变明显减少(P<0.05)。综上所述,本研究表明合成的pet基伤口敷料具有商业应用的能力。
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引用次数: 2
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Ankara Universitesi Veteriner Fakultesi Dergisi
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