Animal Reproduction Science最新文献_第2页

Phosphatidylcholine promotes sperm motility by regulating sperm membrane phospholipid homeostasis 磷脂酰胆碱通过调节精子膜磷脂稳态促进精子运动

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.anireprosci.2026.108108

Jiwei Li , Yuzhu Cao , Juan Zhang , Yadong Tian , Guirong Sun , Yaling Gu , Xiangtao Kang , Donghua Li

The effect of phosphatidylcholine and lysophosphatidylcholine on the maintenance of sperm motility in vitro was investigated. Sperm motility is a key determinant of reproductive efficiency in poultry production, influenced by seminal plasma factors. This study characterized phenotypic distinctions between high- and low-motility sperm from 40-week-old Yufen 1 roosters and elucidated underlying molecular mechanisms. Metabolomics analysis showed that seminal plasma was enriched in glycerophospholipid metabolic pathway, phosphatidylcholine and lysophosphatidylcholine were the main metabolites. The effect of phosphatidylcholine on the maintenance of sperm motility in vitro was tested and verified by sperm co-incubation experiments. Herein, the high-motility group showed significantly higher fertilization capacity when compared to the low-motility sperm (P < 0.05). Furthermore, the removal of seminal plasma impaired motility, while cross-exchange between high- and low-motility groups restored motility (P < 0.05) and marginally improved membrane integrity in low-motility sperm. Metabolomic profiling of seminal plasma revealed the effects of seminal plasma on sperm motility were associated with glycerophospholipid metabolism, lysine degradation, and arachidonic acid metabolism pathways. Among these pathways, The conversion from phosphatidylcholine to lysophosphatidylcholine, mediated by glycerophosphatidylcholine metabolism, becomes the key mechanistic driver. The content of phosphatidylcholine in seminal plasma significantly affects the motility, membrane integrity, and fertilization characteristics (P < 0.05), respectively. These findings underscore the importance of seminal plasma in sperm motility, as well as that phosphatidylcholine probably serves as a key regulator for sperm properties.

研究了磷脂酰胆碱和溶血磷脂酰胆碱对体外维持精子活力的影响。精子活力是家禽繁殖效率的关键决定因素，受精浆因素的影响。本研究研究了40周龄育芬1号公鸡高活力精子和低活力精子的表型差异，并阐明了潜在的分子机制。代谢组学分析表明，精浆中甘油磷脂代谢途径富集，主要代谢产物为磷脂酰胆碱和溶血磷脂酰胆碱。通过精子共孵育实验验证了磷脂酰胆碱对体外维持精子活力的作用。其中，高活力组精子的受精率显著高于低活力组（P <； 0.05）。此外，去除精浆会损害精子的运动能力，而高活力组和低活力组之间的交叉交换恢复了精子的运动能力（P <； 0.05），并略微改善了低活力精子的膜完整性。精浆代谢组学分析显示精浆对精子活力的影响与甘油磷脂代谢、赖氨酸降解和花生四烯酸代谢途径有关。其中，由磷脂酰胆碱代谢介导的磷脂酰胆碱向溶血磷脂酰胆碱的转化成为关键的机制驱动因素。精浆中磷脂酰胆碱含量分别显著影响了卵动力、卵膜完整性和受精特性（P <； 0.05）。这些发现强调了精浆在精子运动中的重要性，以及磷脂酰胆碱可能是精子特性的关键调节剂。

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引用次数: 0

Application of zona-free cloning technologies to livestock breeding programmes 无带克隆技术在家畜育种计划中的应用

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-15 DOI: 10.1016/j.anireprosci.2026.108107

Muren Herrid , Alison Van Eenennaam

Advanced reproductive technologies are powerful tools for accelerating genetic gain because they enable both increased selection intensity and the generation of large numbers of offspring from elite animals. Among these, somatic cell nuclear transfer (SCNT) cloning offers a means to rapidly multiply elite genetics from nucleus herds into commercial populations. Genomic estimated breeding values allow for the accurate assessment of genetic merit in embryos and newborns, creating opportunities to identify elite young stock for cloning. Cloning will also be an important complement to gene-editing as a way to generate animals from cell lines carrying targeted genetic modifications. Nevertheless, the widespread application of cloning remains constrained by low efficiency and high costs, underscoring the need for continued optimisation. Zona-free cloning, also known as handmade cloning, has potential as a simpler and more scalable alternative to conventional micromanipulator-based methods to prepare cloned embryos, and has demonstrated promising improvements in pregnancy and live birth rates across several livestock species. This review summarises advances in zona-free cloning, tracing its development from the microblade to the two-pipette and micropipette methods, and evaluates their relative advantages and limitations. Approaches to improve the health and welfare of clones are examined, and potential applications of cloning in livestock breeding programmes, along with associated regulatory considerations, are discussed. Cloning may become increasingly important as a crucial bridge between lines of genetically elite and possibly gene-edited embryonic cells, and their manifestation as breeding animals in livestock improvement programs.

先进的生殖技术是加速遗传增益的有力工具，因为它们既能提高选择强度，又能从精英动物中产生大量的后代。其中，体细胞核移植（SCNT）克隆提供了一种从核心群体快速繁殖精英遗传到商业群体的手段。基因组估计育种值允许对胚胎和新生儿的遗传优点进行准确的评估，创造机会来确定用于克隆的优秀幼种。克隆也将是基因编辑的重要补充，因为它是一种从携带靶向基因修饰的细胞系中产生动物的方法。然而，克隆的广泛应用仍然受到低效率和高成本的限制，强调需要继续优化。无带克隆，也被称为手工克隆，有可能作为一种更简单和更可扩展的替代方法，以制备基于微操纵器的传统克隆胚胎方法，并已证明有希望改善几种牲畜的怀孕率和活产率。本文综述了无带克隆技术的研究进展，回顾了无带克隆技术从微叶片法到双移液管法和微移液管法的发展历程，并对其优缺点进行了评价。研究了改善克隆动物健康和福利的方法，并讨论了克隆在牲畜育种方案中的潜在应用，以及相关的管理考虑。克隆可能会变得越来越重要，因为它是连接遗传精英和可能经过基因编辑的胚胎细胞的关键桥梁，也是它们在牲畜改良计划中作为繁殖动物的表现。

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引用次数: 0

Comprehensive characterization of transcriptional regulation during HCG-induced follicle maturation in mandarin fish (Siniperca chuatsi): Insights from transcriptomics 在hcg诱导的鳜鱼卵泡成熟过程中转录调控的综合表征：转录组学的见解

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-26 DOI: 10.1016/j.anireprosci.2026.108116

Mingqing Zhang , Zixuan E , Han Wen, Yingshi Tang, Shujia Liao, Lin Tang, Fengying Yan, Yong Zhang, Shuisheng Li

Follicle maturation is critical for egg quality and fry production, yet its transcriptional regulation remains poorly defined. In this study, we employed comparative transcriptomics across five developmental stages (SCS1–SCS5) following HCG induction to resolve stage-specific transcriptional dynamics in mandarin fish (Siniperca chuatsi) follicle. A total of 15 libraries yielded high-quality data with mapping rates of 92.50–96.79 %, identifying 17,320 differentially expressed genes (DEGs), with a pronounced increase in later maturation stages. Functional enrichment highlighted stage-specific activation of key biological processes, including oocyte maturation, steroid biosynthesis, meiotic regulation, lysosomal proteolysis, fatty acid degradation, and immune responses. Notably, core cell-cycle regulators (CDC20, BUB1Bb, PKMYT1, YWHAE1) exhibited dynamic patterns consistent with meiotic resumption, indicating activation of the maturation-promoting factor (MPF) and APC/C pathways. Yolk hydrolysis was mediated by lysosomal proteases (CTSBa, CTSC) and ubiquitin–proteasome system genes (CDC34a), while lipid metabolism supported lipid droplet formation. Stage-specific immune activation further suggested complex immune–reproductive crosstalk. Co-expression network analysis further identified key hub genes positively (e.g., CDC20, BUB1Bb, SETD2) or negatively (e.g., MRPL4, RPS3) associated with maturation. Collectively, this study provides a comprehensive transcriptomic resource and reveals critical pathways and regulators, offering molecular insights to refine hormonal induction and selective breeding strategies in aquaculture.

卵泡成熟对鸡蛋质量和鱼苗产量至关重要，但其转录调控仍不明确。在这项研究中，我们采用了HCG诱导后五个发育阶段（SCS1-SCS5）的比较转录组学来分析鳜鱼卵泡中特定阶段的转录动力学。共有15个文库获得了高质量的数据，作图率为92.50-96.79 %，鉴定出17,320个差异表达基因（deg），在成熟后期显著增加。功能富集强调了关键生物过程的阶段特异性激活，包括卵母细胞成熟、类固醇生物合成、减数分裂调节、溶酶体蛋白水解、脂肪酸降解和免疫反应。值得注意的是，核心细胞周期调节因子（CDC20, BUB1Bb, PKMYT1, YWHAE1）表现出与减数分裂恢复一致的动态模式，表明激活了成熟促进因子（MPF）和APC/C途径。卵黄水解由溶酶体蛋白酶（CTSBa、CTSC）和泛素-蛋白酶体系统基因（CDC34a）介导，脂质代谢支持脂滴形成。阶段特异性免疫激活进一步提示复杂的免疫-生殖串扰。共表达网络分析进一步确定了与成熟相关的关键枢纽基因（如CDC20、BUB1Bb、SETD2）或负相关（如MRPL4、RPS3）。总的来说，本研究提供了全面的转录组资源，揭示了关键途径和调控因子，为完善水产养殖中的激素诱导和选择性育种策略提供了分子见解。

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引用次数: 0

Graphene oxide enhances post-thaw sperm quality in tropically-adapted rams 氧化石墨烯提高热带适应公羊解冻后精子质量。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-29 DOI: 10.1016/j.anireprosci.2026.108119

Francisca N.M. Almeida , Denise D. Guerreiro , Alan M. Araújo , Bruno R.M. Costa , Eduardo R. Pessoa , Gaby J.Q. Palomino , Aderson M. Viana Neto , Luciano P. Silva , Valder N. Freire , Arlindo A. Moura

This study investigates the effects of graphene oxide (GO) on the quality of frozen-thawed ram semen. Each of 30 ejaculates from five rams were cryopreserved with a control extender (Optidux®, Reprodux Inc., Brazil) or extender containing GO concentrations at 5, 50, or 100 µg/mL. Post-thaw data were analyzed using Tukey’s or Dunn’s t test (p < 0.05). Graphene oxide at 5 and 50 µg/mL increased frozen-thawed total (50.4 ± 3.15; 52^.0 ± 2.76 %), and progressive sperm motility (44.2 ± 3.06; 45^.7 ± 2.68 %) vs control (42.0 ± 2.75; 36.7 ± 2.58 %), and sperm viability (56.3 ± 2.17; 57.8 ± 2.82 %) vs control (48.8 ± 2.60 %); and decreased immotile spermatozoa (49.6 ± 3.15; 48.0 ± 2.76 %) vs control (58.0 ± 2.75 %). Both 5 and 100 µg/mL treatments improved DNA integrity (90 ± 0.34; 90.5 ± 0.44 %) vs control (89.1 ± 0.37 %). There were no GO effects on sperm morphology, VCL, VAP. Local motility, an undesirable parameter, increased significantly from 5.42 ± 0.52 % (control) to 6.77 ± 0.43 % (100 µg/mL GO). Supplementation with GO improved post-thaw sperm progressive motility (by up to 24.5 %) and viability (up to 18.4 %), reducing immotile sperm (up to 17.2 %), compared to extender alone. This potentially supports the practical use of GO as an additive to semen extenders.

本研究探讨氧化石墨烯（GO）对冻融公羊精液质量的影响。5只公羊的30次射精分别用对照填充剂（Optidux®，repdux Inc.，巴西）或含有氧化石墨烯浓度为5、50或100 µg/mL的填充剂冷冻保存。Post-thaw数据分析使用图基或邓恩的t测试(p 0 ±2.76  %),和进步精子的运动性(44.2 ± 3.06;45.7 ±2.68  %)和控制(42.0 ± 2.75;36.7 ±2.58  %),和精子活力(56.3 ± 2.17;57.8 ±2.82  %)和控制(48.8 ±2.60  %);与对照组（58.0 ± 2.75 %）相比，不动精子减少（49.6 ± 3.15;48.0 ± 2.76 %）。5和100年 µg / mL治疗改善DNA完整性(90 ± 0.34;90.5 ±0.44  %)和控制(89.1 ±0.37  %)。氧化石墨烯对精子形态、VCL、VAP均无影响。局部运动率（一个不希望的参数）从5.42 ± 0.52 %（对照组）显著增加到6.77 ± 0.43 %（100 µg/mL GO）。与单独添加氧化石墨烯相比，添加氧化石墨烯可提高解冻后精子的进步性（高达24.5% %）和活力（高达18.4% %），减少不动精子（高达17.2% %）。这潜在地支持了氧化石墨烯作为精液填充剂添加剂的实际应用。

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引用次数: 0

Heat stress disrupts the ovarian microenvironment in cattle: An in vivo analysis of hormonal alterations, oxidative stress-induced apoptosis, and reduced pregnancy outcomes 热应激破坏牛卵巢微环境：体内激素改变、氧化应激诱导的细胞凋亡和妊娠结局降低的分析。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-17 DOI: 10.1016/j.anireprosci.2026.108111

Tae-Gyun Kim , Sung-Ho Kim , Seung-Joon Kim , Won-Jae Lee

Heat stress during summer impairs reproductive function in cattle. Since previous studies have largely employed in vitro models to examine heat stress effects on oocytes and mural granulosa cells (MGCs), comprehensive in vivo research is required to elucidate its multifaceted impact on cattle fertility. This study aimed to clarify the mechanisms of heat stress-induced reproductive changes by comparing ovarian microenvironment, hormone levels, and pregnancy rates in cattle across seasons. Compared with the control group (temperature–humidity index [THI] <72, n = 87), the heat-stressed group (HS; THI ≥72, n = 86) exhibited significant alterations in reproductive physiology, including increased serum cortisol concentration, and a reduced pregnancy rate (60.3 % vs. 43.5 %). In the HS group, MGCs showed reduced expression of steroidogenic-related proteins (StAR, LHR, and aromatase), accompanied by reduced estradiol concentrations in follicular fluid. A significant upregulation of oxidative stress-related proteins (SOD1 and SOD2) and the apoptosis marker (caspase-3) was observed, with a positive correlation identified between these factors. These findings suggest that MGCs exposed to heat stress undergo late-stage apoptosis driven by oxidative stress. In the HS group, gene expression in dominant follicle-derived cumulus–oocyte complexes presented an impaired oocyte maturation (decreased GDF9 and BMP15), while immunofluorescence analysis revealed increased HSP70 signal intensity in oocytes. Collectively, these findings suggest that heat stress triggers molecular and hormonal alterations in the ovarian microenvironment, leading to impaired oocyte maturation and reduced fertility. This study provides essential insight for the development of effective strategies to mitigate heat stress and enhance reproductive outcomes in livestock.

夏季的热应激会损害牛的生殖功能。由于先前的研究主要采用体外模型来研究热应激对卵母细胞和壁粒细胞（MGCs）的影响，因此需要全面的体内研究来阐明其对牛生育能力的多方面影响。本研究旨在通过比较不同季节牛卵巢微环境、激素水平和妊娠率，阐明热应激诱导生殖变化的机制。与对照组比较(温湿指数[THI]

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引用次数: 0

The effect of hibernation on reproduction performance of Alligator sinensis, Chinese Alligator is not limited to maintenance of matural follicle, but also involves the fundamental resource of germ cells 冬眠对扬子鳄繁殖性能的影响不仅限于对天然卵泡的维持，还涉及生殖细胞的基本资源

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-26 DOI: 10.1016/j.anireprosci.2026.108115

Haitao Nie , Fuyong You , Shi Wang , Yunlu Xu , Shaofan Li , Jixiang Zhan , Yuan Zhang , Peng Liu , Yue Wen , Yongkang Zhou , Song Zhang , Xiaobing Wu

Hibernation, acknowledged as a survival strategy under low-temperatures, is regarded as exert a substantial impact on reproductive performance. However, numerous enigmas remain concerning the underlying mechanisms of this interaction. Ultrasound scanning was employed to quantify follicle numbers, and assay of plasma neurotransmitters and steroid hormones were conducted to elucidate the dynamic correlations between follicle development and endocrine fluctuations during the hibernation period, encompassing onset of hibernation (OH), deep hibernation (DH), and ending of hibernation (EH). The findings demonstrated that a cycle of follicular development commenced prior to the OH, and an additional cycle of yolk deposition occurred subsequent to hibernation. Furthermore, a notable correlation was identified between the quantity of follicles larger than 20 mm (PF, the one closest to mature size) and the plasma GABA/glutamine concentration. This strongly suggests that the maintenance of follicles nearing maturity might be associated with the fluctuations of neurotransmitters during hibernation. The RNA-seq results indicate that the down-regulation of GABA receptors in PF may imply a reduced neurotransmitter responsiveness, a phenomenon that is temporally congruent with the hibernation period. We postulate that this phenomenon might function to prevent excessive cell proliferation and apoptosis, potentially facilitating their continuous existence during hibernation. The findings from MeRIP-seq in conjunction in vitro molecular responses following sh-METTL3 treatment, further indicate that the suppression of oocyte recruitment into vitellogenic growth during hibernation is correlated with the M⁶A methylation modification. Additionally, the corresponding results imply that, beyond the initiation and recruitment of primordial follicles, the ovarian reserve may also been potentially influenced by the hibernation depth through crucial biological events such as double-strand breaks (DSBs) and the synapsis of homologous chromosomes during oogenesis.

冬眠被认为是低温环境下的一种生存策略，对繁殖性能有重要影响。然而，关于这种相互作用的潜在机制仍然存在许多谜团。采用超声扫描定量卵泡数量，血浆神经递质和类固醇激素测定，阐明冬眠期间卵泡发育与内分泌波动的动态关系，包括冬眠开始（OH）、深度冬眠（DH）和冬眠结束（EH）。研究结果表明，卵泡发育周期在卵泡发生前开始，卵黄沉积周期在冬眠后发生。此外，在大于20 mm （PF，最接近成熟尺寸）的卵泡数量与血浆GABA/谷氨酰胺浓度之间存在显著相关性。这强烈表明卵泡接近成熟的维持可能与冬眠期间神经递质的波动有关。RNA-seq结果表明，PF中GABA受体的下调可能意味着神经递质反应性降低，这一现象在时间上与冬眠期一致。我们假设这种现象可能起到防止细胞过度增殖和凋亡的作用，潜在地促进它们在冬眠期间的持续存在。MeRIP-seq研究结果结合sh-METTL3处理后的体外分子反应进一步表明，冬眠期间卵母细胞募集到卵黄细胞生长的抑制与M6A甲基化修饰相关。此外，相应的结果表明，除了原始卵泡的起始和募集，卵巢储备也可能通过卵发生过程中双链断裂（DSBs）和同源染色体突触等关键生物学事件受到冬眠深度的潜在影响。

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引用次数: 0

The Pma-PABP4 gene, regulated by Pma-miR-1444, participates in Pinctada fucata gonad development Pma-miR-1444调控的Pma-PABP4基因参与中华绒螯虾性腺发育

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-11 DOI: 10.1016/j.anireprosci.2026.108106

Ying Guo , Lirong Bai , Pei Wang , Tangrongjun Guo , Ruolin Zhu , Weiwei Chen , Xiuxiu Sang , Xianwei Fan , Dahui Yu

This study cloned and characterized the full-length cDNA of the Pma-PABP4 gene from Pinctada fucata, which is 3123 bp in length and encodes a 641-amino acid protein. The protein exhibited typical RNA-binding protein characteristics, including four RNA recognition motifs (RRMs) and a poly(A)-binding domain. Bioinformatics analyses revealed that Pma-PABP4 is hydrophobic and contains multiple predicted phosphorylation sites. Phylogenetic analysis showed that Pma-PABP4 is highly homologous to PABP4 from other mollusks and shares evolutionary conservation with human PABP1. Additionally, Pma-PABP4 was widely expressed in many tissues in P. fucata with sex-biased expression, where expression levels varied significantly during early embryonic development and specific stages of gonadal development. It was also localized in the gonadal tissues, and RNA interference experiments confirmed that silencing Pma-PABP4 resulted in abnormal gonadal development and reduced gamete production. This study also showed that Pma-miR-1444 directly targets the coding sequence of Pma-PABP4 at the 1371-bp site, negatively regulating its expression. The spatiotemporal expression patterns of Pma-miR-1444 were also closely associated with gametogenesis and embryonic development. Overexpression of Pma-miR-1444 led to gonadal atrophy and a significant reduction in gamete numbers. In conclusion, Pma-miR-1444 and Pma-PABP4 play crucial regulatory roles in the reproductive development and embryogenesis of P. fucata.

本研究克隆并鉴定了fucata pintada Pma-PABP4基因全长cDNA，全长3123 bp，编码641个氨基酸的蛋白。该蛋白具有典型的RNA结合蛋白特征，包括4个RNA识别基序（rrm）和一个聚(a)结合结构域。生物信息学分析显示Pma-PABP4是疏水性的，含有多个可预测的磷酸化位点。系统发育分析表明，Pma-PABP4与其他软体动物的PABP4高度同源，与人类PABP1具有相同的进化保守性。此外，Pma-PABP4在fucata的许多组织中广泛表达，并具有性别偏倚表达，其表达水平在胚胎早期发育和性腺发育的特定阶段发生显著变化。它也定位于性腺组织，RNA干扰实验证实，沉默Pma-PABP4会导致性腺发育异常和配子产生减少。本研究还发现，Pma-miR-1444直接靶向Pma-PABP4编码序列的1371-bp位点，负向调控其表达。Pma-miR-1444的时空表达模式也与配子体发生和胚胎发育密切相关。Pma-miR-1444的过表达导致性腺萎缩和配子数量的显著减少。综上所述，Pma-miR-1444和Pma-PABP4在fucata的生殖发育和胚胎发生中起着至关重要的调节作用。

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引用次数: 0

Dihydrotestosterone binding to the androgen receptor inhibits apoptosis in ovine undifferentiated granulosa cells via AMH signaling 双氢睾酮结合雄激素受体通过AMH信号抑制绵羊未分化颗粒细胞凋亡

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.anireprosci.2026.108105

Yufen Zhao , Haijiang Liu , Zhe Mu , Haijun Li

Although dihydrotestosterone (DHT), androgen receptor (AR), and anti-Müllerian hormone (AMH) play key roles in ovarian function, their precise contributions and synergistic roles in mammalian follicular development remain unclear. In this study, we first evaluated whether ovine granulosa cells (GCs) undergo differentiation during vitro culture and then determined methods for inhibiting differentiation. Following the culture of GCs with DHT (10⁻⁷ mol/L) and the AR antagonist enzalutamide (ENZ) (10⁻⁶ mol/L) separately or in combination for 48 h, an apoptosis assay revealed that the interaction between DHT and AR decreases sheep GC apoptosis. DHT significantly increased the AMH concentration through AR signaling. In addition, DHT and AR significantly increased the intracellular AMH level, and the use of rAMH (70 pg/mL), a sheep recombinant AMH, significantly upregulated the expression of the BCL2 mRNA in GCs. In summary, GCs undergo spontaneous differentiation during in vitro culture, and the use of the specific luteinizing hormone receptor (LHR) antagonist BAY-899 to inhibit their differentiation establishes an in vitro culture model that maintains GC characteristics. Thus, the interaction between DHT and AR may be involved in the accumulation of AMH signals, affecting GC apoptosis. We provide new mechanistic insights into the roles of DHT and AR in attenuating sheep GC apoptosis. The finding that potential cross-points between DHT/AR-induced intracellular signals affect GC apoptosis will help elucidate the mechanisms regulating early follicular development.

尽管双氢睾酮（DHT）、雄激素受体（AR）和抗勒氏激素（AMH）在卵巢功能中起着关键作用，但它们在哺乳动物卵泡发育中的确切作用和协同作用尚不清楚。在这项研究中，我们首先评估了绵羊颗粒细胞（GCs）在体外培养过程中是否发生分化，然后确定了抑制分化的方法。DHT（10−7 mol/L）和AR拮抗剂恩扎鲁胺（enzz）（10−6 mol/L）分别或联合培养GCs 48 h后，细胞凋亡实验显示DHT和AR相互作用可减少绵羊GC细胞凋亡。DHT通过AR信号通路显著提高AMH浓度。此外，DHT和AR显著提高了细胞内AMH水平，rAMH(70 pg/mL)，一种绵羊重组AMH，显著上调了GCs中BCL2 mRNA的表达。综上所述，GC在体外培养过程中自发分化，利用特异性黄体生成素受体（LHR）拮抗剂BAY-899抑制其分化，建立了维持GC特征的体外培养模型。因此，DHT与AR的相互作用可能参与了AMH信号的积累，影响了GC的凋亡。我们为DHT和AR在减轻绵羊GC细胞凋亡中的作用提供了新的机制见解。DHT/ ar诱导的细胞内信号之间的潜在交叉点影响GC凋亡的发现将有助于阐明调节早期卵泡发育的机制。

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引用次数: 0

Effect of rearing environment and age on spermatophore quality in Penaeus indicus and validation of short term storage extenders 饲养环境和饲养年龄对印度对虾精包质量的影响及短期贮存剂的验证。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-22 DOI: 10.1016/j.anireprosci.2026.108114

Biju Francis , C.P. Balasubramanian , P.S. Shyne Anand , Akshaya Panigrahi , Jose Antony , N.S. Sudheer , R. Aravind , K.K. Vijayan

The reproductive development of male Penaeus indicus was studied in indoor tanks at three stocking densities (T1–15, T2–30 and T3–60/m²) and outdoor polythene lined ponds (SD-10/m²) for 180 days. Spermatophore development and the quality changes in relation to age was studied and compared with wild caught shrimps. Pond-reared males exhibited fused petasma and presence of visible spermatophores in the terminal ampoulle, at a body weight of 12.23 ± 0.13 g (90 DOC) indicating morphological maturity. Tank reared males exhibited poor growth and inferior spermatophore quality was observed irrespective of stocking density. Spermatophore weight (0.146 ± 0.015 g) and sperm count (21.47 ± 1.97 × 10⁶ cells) increased significantly with age (180 DOC) in pond-reared males indicating superior sperm quality than tank reared males (180 DOC). Wild males exhibited significantly higher sperm count (43.05 ± 8.05 × 10⁶ cells), morphologically normal cells, and acrosome reaction than pond reared males (180 DOC), while spermatophore weight remained statistically similar between the groups. Spermatophore preservation in three extenders (mineral oil, phosphate buffer (PBS), and calcium-free saline (CFS)) were evaluated during 15 day period. Mineral oil showed superior preservation of sperm viability and acrosome integrity during the first 5–10 days. The findings conclude that, outdoor pond rearing enhances male reproductive quality in P. indicus compared to indoor tank rearing, particularly in males at 180 DOC and using mineral oil as extender can improve retention of sperm cell viability during short term preservation.

研究了3种放养密度（T1-15、T2-30和T3-60/m²）的室内池和室外聚乙烯衬里池（SD-10/m²）的雄性印度对虾（Penaeus indicus）生殖发育180 d。研究了野生捕虾精子包囊的发育和质量随年龄的变化，并与野生捕虾进行了比较。池塘饲养的雄性表现出融合的足部，在末端壶中存在可见的精囊，体重为12.23 ± 0.13 g (90 DOC)，表明形态成熟。无论放养密度如何，池养雄鼠均表现出较差的生长和较差的精包质量。塘养雄鱼精子包囊重量（0.146 ± 0.015 g）和精子数量（21.47 ± 1.97 × 10⁶细胞）随年龄（180 DOC）显著增加，表明其精子质量优于池养雄鱼（180 DOC）。野生雄鱼的精子数量（43.05 ± 8.05 × 10⁶细胞）、细胞形态正常、顶体反应均显著高于塘养雄鱼（180 DOC），而精包重量在两组间无统计学差异。在15 天的时间内，研究了三种填充剂（矿物油、磷酸盐缓冲液（PBS）和无钙生理盐水（CFS））对精子包囊的保存情况。矿物油在头5-10天内对精子活力和顶体完整性有较好的保存作用。综上所述，室外池养比室内池养能提高籼籼虫的雄性生殖质量，特别是在180 DOC时，使用矿物油作为延长剂可提高短期保存期间的精子活力。

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引用次数: 0

Retrospective evaluation of semen quality in bulls in Spain, managed in extensive grazing systems over a 10-year period 回顾性评价公牛精液质量在西班牙，管理在广泛放牧系统10年期间。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2026-04-01 Epub Date: 2026-01-31 DOI: 10.1016/j.anireprosci.2026.108120

C.C. Pérez-Marín , L. Quevedo , M. Salas , L.A. Quintela

Extensively managed beef herds often lack systematic reproductive monitoring, which can lead to reduced fertility rates and economic losses. Bull Breeding Soundness Evaluation (BBSE) is a critical tool for identifying sires with suboptimal reproductive performance. This study aimed to assess semen quality and reproductive soundness in beef bulls raised under extensive systems, to determine the prevalence of unsatisfactory breeders and identify factors affecting reproductive performance. A retrospective analysis was conducted on 1541 evaluations for breeding soundness in 549 bulls (checked in different years or re-checked after an unsatisfactory result) across 45 farms in southern Spain between 2014 and 2024. Bulls underwent physical examination, scrotal circumference (SC) measurement, and semen collection (by electroejaculation). Sperm motility was assessed using computer-assisted analysis. Bulls were classified as suitable, questionable or unsatisfactory based on BBSE criteria. Overall, 83.1 % of bulls passed BBSE: 68.9 % were classified as suitable and 14.2 % as questionable. A total of 16.9 % were unsatisfactory due to low progressive motility, anatomical defects, or clinical conditions. Morphological sperm assessment was not performed in all the samples. A total of 230 bulls classified as unsatisfactory in a first evaluation were reevaluated (63.5 %), showing that 52.9 % of them exhibited acceptable semen quality later, highlighting the importance of follow-up testing. Limousin bulls had the highest probability of passing BBSE (82.5 %), while older bulls (>85 months) had reduced odds. Seminal parameters varied significantly among breeds, and age affected SC but not sperm motility or other semen parameters. BBSE is an essential tool in extensive systems to ensure optimal bull fertility and prevent economic losses. Repeated evaluations improve accuracy, particularly for bulls initially deemed unsatisfactory. Breed, age, and farm management practices significantly influence reproductive outcomes and should be considered in breeding programs.

广泛管理的肉牛群往往缺乏系统的生殖监测，这可能导致生育率下降和经济损失。公牛繁育健全性评价（BBSE）是鉴定繁殖性能不理想的公牛的重要工具。本研究旨在评估粗放型系统饲养的肉牛的精液质量和生殖健康状况，以确定不满意育种者的普遍程度，并确定影响生殖性能的因素。2014年至2024年，对西班牙南部45个农场的549头公牛（不同年份检查或结果不满意后重新检查）的1541次繁殖健全性评估进行了回顾性分析。公牛接受了体格检查、阴囊周长（SC）测量和精液采集（通过电射精）。使用计算机辅助分析评估精子活力。根据疯牛病标准，公牛被分为合适、可疑或不满意。总的来说，83.1 %的公牛通过了疯牛病，68.9% %被归类为合适，14.2% %被归类为可疑。16.9 %的患者由于进行性运动能力低、解剖缺陷或临床条件不满意。未对所有样本进行精子形态评估。在第一次评估中被分类为不满意的共有230头公牛被重新评估（63.5 %），表明52.9% %的公牛后来表现出可接受的精液质量，突出了后续测试的重要性。利穆赞公牛通过BBSE的概率最高（82.5 %），而年龄较大的公牛（bb0 85个月）的几率较低。不同品种的精液参数差异显著，年龄影响SC，但不影响精子活力或其他精液参数。在广泛的系统中，BBSE是确保最佳公牛生育力和防止经济损失的重要工具。反复评估可以提高准确性，特别是对于最初被认为不满意的公牛。品种、年龄和农场管理措施对繁殖结果有显著影响，应在育种计划中予以考虑。

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引用次数: 0