Animal Reproduction Science最新文献_第5页

Histomorphometric characterization of the endometrium in mules (Equus mulus): An approach to endometritis/endometrosis 骡子子宫内膜的组织形态学特征：子宫内膜炎/子宫内膜异位症的一种方法。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108056

Miguel A. Gutiérrez-Reinoso , Pedro M. Aponte , Manuel García-Herreros , Katlhen G. García-Bravo , Daniela Rojas , Yat Sen Wong , Fernando Saravia , Fidel Ovidio Castro , Jaime Catalán , Jordi Miró

Mules (Equus mulus), as sterile hybrids between mares and donkeys, present a unique uterine morphology and physiology that remains poorly characterized. This study provides the first histomorphometric evaluation and transcriptional profiling of the endometrium in adult mules. To achieve this, endometrial biopsies were analysed using quantitative stereology, histological classification (Kenney–Doig system), and qPCR of key immune-related and fibrotic genes. Histometric analyses revealed that the stratum spongiosum was the predominant component of the mule endometrium (mean volume density: 84.7 %), whereas the epithelium and compact layers accounted for smaller proportions. This distribution, more pronounced than what is typically reported in mares, suggests enhanced stromal expansion or edema. Endometrial glands were abundant, and the overall structural profile exhibited an intermediate phenotype with a clear asinine bias. Most samples were classified as grades I, IIA, or IIB, with no specimens reaching grade III. Mild to moderate stromal remodeling, preserved tissue architecture, and inflammatory signatures –characterized by increased relative abundance of IL1B, IL6, and TNFα transcripts– were observed in higher grades and were consistent with early stages of endometrial degeneration. Although these features indicate early degenerative changes, the hybrid reproductive physiology of mules, including limited hormonal cyclity, may contribute to reduced progression towards fibrosis. This integrated histological–molecular assessment reinforces the diagnostic value of the Kenney–Doig system in mules and supports the adaptation of a refined IIC grade to better capture hybrid-specific features. Overall, the mule endometrium displays distinct yet comparable traits relative to that of horses and donkeys, highlighting its potential as a comparative model for equine reproductive research. These findings provide a foundation for improved clinical decision-making and future studies on hybrid fertility and uterine health.

骡子（Equus mulus）是母马和驴之间的不育杂种，具有独特的子宫形态和生理特征。这项研究首次提供了成年骡子子宫内膜的组织形态学评估和转录谱分析。为了实现这一点，使用定量立体学、组织学分类（kennedy - doig系统）和关键免疫相关基因和纤维化基因的qPCR对子宫内膜活检进行分析。组织计量学分析显示，海绵层是骡子子宫内膜的主要成分（平均体积密度：84.7 %），而上皮和致密层占较小的比例。这种分布比母马的典型报告更为明显，表明间质扩张或水肿增强。子宫内膜腺丰富，整体结构表现出明显的asinine偏倚的中间表型。大多数标本被划分为I、IIA或IIB级，没有标本达到III级。轻度至中度间质重塑、保留的组织结构和炎症特征——以IL1B、IL6和TNFα转录物相对丰度增加为特征——在更高级别的子宫内膜变性中被观察到，并且与早期阶段一致。尽管这些特征表明早期退行性改变，但骡子的杂交生殖生理，包括有限的激素周期，可能有助于减少纤维化的进展。这种综合的组织学-分子评估强化了kennedy - doig系统在骡子中的诊断价值，并支持改进的IIC分级，以更好地捕捉杂交特异性特征。总的来说，与马和驴相比，骡子的子宫内膜显示出不同但可比较的特征，突出了其作为马生殖研究的比较模型的潜力。这些发现为改进临床决策和进一步研究杂交生育和子宫健康提供了基础。

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引用次数: 0

Effect of post-mating TAK-683 (Kisspeptin analog) treatment on luteal morphology and function in suckling goats with lactational anestrus 交配后处理TAK-683 （Kisspeptin类似物）对哺乳期停发山羊黄体形态和功能的影响。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108052

Gökhan Uyanık , Murat Yüksel , Murat Abay , Ahmet Gözer , Ufuk Kaya , Filiz Kara , Ebru Arslanhan , Ishak Gökçek

This study aimed to evaluate the effects of TAK-683 administered to suckling goats in lactational anestrus on the 4th day (D4) after mating on luteal function, serum progesterone (P₄) levels, and reproductive performance. Forty-five Aleppo goats were treated with intravaginal sponges containing medroxyprogesterone acetate for 7 days and were injected intramuscularly with 500 IU eCG and 75 µg d-cloprostenol on the day of sponge removal. Thirty-seven goats showed estrus and were mated with fertile bucks (D0). The mated goats were randomly divided into groups to receive subcutaneous administration of saline (Control, n = 12), 5 μg TAK-683 (TAK-5, n = 12) or 25 μg TAK-683 (TAK-25, n = 13) on D4. Transrectal ovarian ultrasonography and blood sampling were performed on D4, D7, D14, D17, and D21, and pregnancy was diagnosed on D35. Regardless of dose, TAK-683 treatment induced the formation of accessory corpora lutea (aCL) and significantly increased original CL (oCL) diameter, luteal area (LA), and Doppler area (DA) compared with controls (P < 0.001). Total LA and DA were greater (P < 0.001) in TAK-5 and TAK-25, although DA/LA ratio did not differ (P > 0.05). Serum P₄ levels were significantly higher in TAK-25 from D7 to D21 (P < 0.001), while TAK-5 showed a delayed increase from D17. Luteal morphological parameters were strongly and positively correlated with circulating P₄ levels, confirming the luteotropic effect of TAK-683. TAK-683 treatment had no significant effect on fertility parameters (P < 0.05). In conclusion, although TAK-683 treatment enhanced luteal function and increased circulating P₄ concentrations, it failed to improve fertility outcomes in suckling goats under lactational anestrus conditions.

本研究旨在评价TAK-683在配种后第4天（D4）给药对哺乳山羊黄体功能、血清孕酮（硫酸铵）水平和繁殖性能的影响。选取45只阿勒颇山羊，用含醋酸甲孕酮的阴道内海绵治疗7 d，取海绵当日肌内注射500 IU eCG和75 µg d-氯前列醇。37只山羊出现发情，并与可育雄鹿交配（D0）。将配对山羊随机分为各组，分别于D4日皮下注射生理盐水（对照组，n = 12）、5 μg TAK-683 （TAK-5, n = 12）或25 μg TAK-683 （TAK-25, n = 13）。于D4、D7、D14、D17、D21行经直肠卵巢超声及采血，D35诊断妊娠。无论剂量如何，TAK-683处理均诱导副黄体（aCL）的形成，与对照组相比，原始CL （oCL）直径、黄体面积（LA）和多普勒面积（DA）显著增加（P  0.05）。从D7到D21， TAK-25患者血清中硫酸磷水平显著升高(P

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引用次数: 0

A new urinary biomarker reflecting the progesterone excretion pattern identified through serum steroid and urinary progesterone metabolite analysis during pro-oestrus and oestrus in female dogs (Canis familiaris) 一种新的反映孕酮排泄模式的尿液生物标志物，通过对母狗（犬）发情前和发情期间血清类固醇和尿孕酮代谢物的分析确定。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-02 DOI: 10.1016/j.anireprosci.2025.108054

Tia M. Pennanen , Merja R. Häkkinen , Risto O. Juvonen , Jouko J. Vepsäläinen , Seppo Auriola , Sari M. Viitala

In female dogs, the optimal breeding time is estimated by an increase in the serum progesterone hormone concentration. The method is not only invasive and unpleasant to dogs but also laborious and requires professional laboratory expertise. The main aim of this study was to explore potential urinary progesterone metabolites for non-invasive oestrus monitoring. Additionally, thirteen steroid hormones, such as sex steroids and corticosteroids, were determined in serum samples from nine female dogs via multiple reaction monitoring liquid chromatographymass spectrometry (LCMS). In addition, various urinary steroid metabolites from 22 dogs were analysed via nontargeted liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis. The serum oestrone, oestradiol, and progesterone concentration profiles were aligned with the existing literature. When the 17β-oestradiol secretion peak (49–83 pg/mL) during pro-oestrus was considered as Day 0, 17α-hydroxyprogesterone, testosterone, and androstenedione concentrations peaked on Days 0–3 (P-values 0.004, 0.015 and 0.007, respectively). Results of corticosteroids unveiled novel insights into steroid secretion during the breeding season of dogs. Parallel urine samples revealed that progesterone was excreted into the urine as the unidentified pregnanetriol glucuronide “PtGX” instead of the common metabolite pregnanediol-3-glucuronide (PdG) among mammals. The excretion profiles of pregnanetriol glucuronide support its usability as a biomarker in non-invasive oestrus monitoring in female dogs. The findings presented herein lay the foundation for a more animal-friendly and streamlined approach to monitoring the oestrus cycle of dogs, with substantial implications for dog breeding and advancing animal welfare practices.

在母狗中，通过增加血清黄体酮激素浓度来估计最佳繁殖时间。这种方法不仅有侵入性，而且对狗狗来说很不舒服，而且很费力，需要专业的实验室知识。本研究的主要目的是探索用于无创发情监测的潜在尿孕酮代谢物。此外，通过多重反应监测液相色谱-质谱法（LCMS）测定了9只母狗血清样本中的13种类固醇激素，如性类固醇和皮质类固醇。此外，通过非靶向液相色谱-高分辨率质谱（LC-HRMS）分析了22只狗的各种尿液类固醇代谢物。血清雌激素、雌二醇和黄体酮浓度谱与现有文献一致。将17β-雌二醇分泌高峰（49 ~ 83 pg/mL）视为发情期第0天，17α-羟孕酮、睾酮和雄烯二酮浓度在第0 ~ 3天达到峰值（p值分别为0.004、0.015和0.007）。皮质类固醇的结果揭示了在狗的繁殖季节类固醇分泌的新见解。平行尿液样本显示，在哺乳动物中，孕酮以未识别的妊娠三醇-葡糖苷“PtGX”的形式排入尿液，而不是常见的代谢物妊娠二醇-3-葡糖苷（PdG）。孕三醇葡萄糖醛酸酯的排泄谱支持其作为雌性犬无创发情监测生物标志物的可用性。本文提出的研究结果为采用一种更有利于动物的、简化的方法来监测狗的发情周期奠定了基础，对狗的繁殖和推进动物福利实践具有重大意义。

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引用次数: 0

Resveratrol's therapeutic role in heat stress-induced mitochondria and ER dysfunction in animal reproductive health: A review 白藜芦醇在热应激诱导的线粒体和内质网功能障碍动物生殖健康中的治疗作用综述

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-01 DOI: 10.1016/j.anireprosci.2025.108044

Muhammad Tariq , Abdul Quddus , Kebede Habtegiorgis Beshah , Dagan Mao

Heat stress is a major environmental factor that causes oxidative stress, mitochondrial dysfunction, and endoplasmic reticulum (ER) stress, which may lead to fertility impairment, decreased fetal growth, and low survival rates. Resveratrol is an antioxidant polyphenolic compound which has been found to have potential in mitigating the damage caused by heat stress. This review explores the therapeutic role of resveratrol in reducing mitochondrial and ER dysfunction in animal reproduction, focusing on its role in enhancing reproductive performance under heat stress. The systematic analysis of the recent studies demonstrates that resveratrol enhances mitochondrial biogenesis, increases mitochondrial dynamics via balanced fission and fusion, and controls the unfolded protein response (UPR), which reduces ER stress. Moreover, resveratrol triggers the SIRT1/AMPK signal transduction pathway that is involved in cellular stress defense and metabolic stability. This review identifies experimental evidence of resveratrol to protect cells related to reproduction against oxidative damage, maintain mitochondrial integrity, and abate apoptosis. Evidence shows that the antioxidant and anti-inflammatory effects of resveratrol are core to its protective effect, and it may be a good potential therapeutic agent enhancing reproductive health and pregnancy outcomes in animals under heat stress. Since it has potential of restoring cellular function and enhancing reproductive efficiency, additional studies should be conducted to maximize dosages, routes of delivery, and species-specific use, especially in livestock and other animals of economic value.

热应激是引起氧化应激、线粒体功能障碍和内质网（ER）应激的主要环境因素，可导致生育能力受损、胎儿生长下降和低存活率。白藜芦醇是一种抗氧化剂多酚化合物，已被发现具有减轻热应激造成的损害的潜力。本文综述了白藜芦醇在降低动物生殖线粒体和内质网功能障碍中的治疗作用，重点介绍了其在热应激下提高生殖性能的作用。近期研究的系统分析表明，白藜芦醇可以促进线粒体生物发生，通过平衡裂变和融合增加线粒体动力学，并控制未折叠蛋白反应（UPR），从而减少内质网应激。此外，白藜芦醇触发SIRT1/AMPK信号转导通路，参与细胞应激防御和代谢稳定。本综述确定了白藜芦醇保护生殖相关细胞免受氧化损伤、维持线粒体完整性和减缓细胞凋亡的实验证据。有证据表明，白藜芦醇的抗氧化和抗炎作用是其保护作用的核心，可能是一种改善热应激动物生殖健康和妊娠结局的潜在治疗药物。由于它具有恢复细胞功能和提高生殖效率的潜力，应进行进一步的研究，以最大限度地增加剂量、给药途径和特定物种的使用，特别是对牲畜和其他有经济价值的动物。

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引用次数: 0

An overview of anti-Müllerian hormone (AMH) in cattle reproduction and its applications in assisted reproductive technologies 抗<s:1>勒氏杆菌激素（AMH）在牛生殖中的作用及其在辅助生殖技术中的应用综述

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-01 DOI: 10.1016/j.anireprosci.2025.108055

Prachi Sharma , K.K. Hadiya , S.S. Layek , S.P. Patil , K. Karuppanasamy , K.B. Raval , S. Gorani , B.N. Saripadiya

Anti-Müllerian hormone was identified by Alfred Jost in the 1950s. This hormone induces regression of Müllerian ducts in male embryos and regulates folliculogenesis by reducing follicular sensitivity to follicle-stimulating hormone (FSH) in females. Recent research suggests Anti-Müllerian hormone (AMH) is a marker for the early selection of cattle with superior reproductive traits in the dairy industry. Studies show a positive correlation between AMH concentration and fertility parameters, such as follicular count, oocyte recovery, and embryo return rates. The importance of AMH in assisted reproductive technologies in humans is well established in humans, the hormone has been similarly investigated in cattle. Anti-Müllerian hormone concentration correlates with superovulation response and embryo production, suggesting utility in donor selection and optimizing assisted reproductive techniques (ART) outcomes. However, variations in AMH levels across breeds and ages emphasize the need for further research to establish standardized thresholds and protocols. Overall, this review highlights the significance of AMH in cattle reproduction and its potential applications in improving reproductive efficiency and genetic selection.

抗勒勒激素是阿尔弗雷德·约斯特在20世纪50年代发现的。这种激素可以诱导雄性胚胎中卵泡导管的退化，并通过降低雌性卵泡对促卵泡激素（FSH）的敏感性来调节卵泡的发生。最近的研究表明，抗勒氏杆菌激素（AMH）是乳品行业早期选择具有优良繁殖性状的牛的标志。研究表明，AMH浓度与卵泡计数、卵母细胞恢复和胚胎返回率等生育参数呈正相关。AMH在人类辅助生殖技术中的重要性在人类中得到了充分证实，这种激素在牛身上也进行了类似的研究。抗勒氏杆菌激素浓度与超排卵反应和胚胎产生相关，提示在供体选择和优化辅助生殖技术（ART）结果方面的效用。然而，不同品种和年龄AMH水平的差异强调需要进一步研究以建立标准化的阈值和方案。综上所述，本文综述了AMH在牛繁殖中的重要意义及其在提高繁殖效率和遗传选择方面的潜在应用。

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引用次数: 0

Deslorelin influences canine epididymal gene expression of the androgen receptor and prostaglandin metabolism 地氯瑞林影响犬附睾雄激素受体基因表达和前列腺素代谢。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-01 DOI: 10.1016/j.anireprosci.2025.108051

Henriette Greiner , Eva-Maria Packeiser , Hanna Körber , Sabrina Stempel , Fritjof Freise , Sandra Goericke-Pesch

Deslorelin slow-release implants (DSRI) reversibly suppresses testicular function in dogs, although the effects on the epididymis remain poorly investigated. As prostaglandins E2 and D2 contribute to epididymal function, presumably regulated by androgens, corresponding metabolic enzymes and receptors were examined in an explorative study. Epididymides of three untreated dogs (CG) were compared with five epidymides obtained by unilateral hemicastration after five months of DSRI treatment (TG), and with the recovered epididymis of the other side, collected five months after implant removal (RG). Gene expressions (RT-qPCR) of the androgen receptor (AR), the synthases PTGS2, PTGES, L-PGDS, and H-PGDS, the receptors EP2, EP4, and DP, as well as prostaglandin inactivator HPGD were compared between groups (CG/TG/RG) and epididymal segments (head/body/tail). Protein localization was evaluated immunohistochemically for AR, PTGS2, HPGD, PTGES, EP2, and EP4. Gene expression differed between TG and CG for all genes except for AR (all P < 0.05), with increased expression in the head (PTGS2, HPGD, PTGES, EP4, HPGDS, and DP). Surprisingly, the only significant difference between RG and TG or CG was a higher PTGS2 expression in the tail in CG (P < 0.05). Friedman tests revealed segment-specific gene expressions within TG (DP), RG (AR, HPGD, EP2, EP4, and L-PGDS), and CG (AR, EP2, L-PGDS) (all P < 0.05), indicating segment-specific function, although without significance in post-hoc tests. Specific immunostaining was confirmed in all samples. Unexpectedly, the DSRI did not alter AR expression, but reversibly affected genes expression within the prostaglandin metabolism. Due to the low sample size, this conclusion must be considered preliminary.

地氯雷林缓释植入物（DSRI）可逆地抑制狗的睾丸功能，尽管对附睾的影响仍然知之甚少。由于前列腺素E2和D2参与附睾功能，可能受到雄激素的调节，因此在探索性研究中检测了相应的代谢酶和受体。将3只未治疗犬（CG）的附睾与DSRI治疗5个月（TG）后单侧麻醉获得的5只附睾进行比较，并与植入物取出5个月（RG）后恢复的另一侧附睾进行比较。比较各组（CG/TG/RG）和附睾节段（头/体/尾）雄激素受体（AR）、合成酶PTGS2、PTGES、L-PGDS和H-PGDS、受体EP2、EP4和DP以及前列腺素失活剂HPGD的基因表达量（RT-qPCR）。用免疫组织化学方法评估AR、PTGS2、HPGD、PTGES、EP2和EP4的蛋白定位。除AR基因外，TG与CG基因表达均存在差异（P < 0.05）

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引用次数: 0

Estradiol esters are dispensable in progesterone-based TAI protocols for Bos taurus suckling beef cows 在以黄体酮为基础的黄牛乳牛TAI方案中，雌二醇是不可缺少的。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-12-01 DOI: 10.1016/j.anireprosci.2025.108050

Alinne Machado Petrarca Léo , Fabiane Pereira de Moraes , Monique Tomazele Rovani , Rogério Ferreira , José Nélio de Sousa Sales , Rafael Gianella Mondadori , Bernardo Garziera Gasperin

Timed artificial insemination (TAI) protocols in South America are mainly based on progesterone (P4) and estradiol (E2). Considering the restrictions on E2 use, the study aimed to evaluate its replacement with GnRH. Multiparous suckling Bos taurus cows (n = 455) were pre-synchronized and received a P4 (1 g) intravaginal device (IVD) on D0. Also, on D0, cows in the Control group received 2 mg of estradiol benzoate IM and cows in the GnRH/GnRH and GnRH/EC groups received 20 µg of buserelin acetate IM. At IVD removal (D7), all cows received 500 µg sodium cloprostenol IM, 300IU eCG IM, and tail paint. At this time, cows in the Control and GnRH/EC groups received 1 mg estradiol cypionate (EC) IM. TAI was performed on D9 (48 h after IVD removal) in all cows, and 10 µg of buserelin acetate IM was administered only in GnRH/GnRH group. No difference was observed among Control (n = 85), GnRH/EC (n = 82) and GnRH/GnRH (n = 84) regarding largest follicle (LF) diameter on D7 (7.3 ± 0.2, 7.6 ± 0.3 and 7.6 ± 0.3 mm, respectively; P = 0.75) and percentage of CL on D7 (87.1, 80.5 and 81.0 %. respectively; P = 0.37). Significant effects of D7 LF diameter (P < 0.0001; odds ratio 1.58) and D7 LF by group interaction on pregnancy rate were observed (P < 0.01). No difference was observed in the estrus rate before TAI (D9; 72.1, 62.4 and 67.9 %; P = 0.22) or in the pregnancy rate (56.9, 53.1 and 53.6 %; P = 0.77) for control (n = 153), GnRH/EC (n = 147) and GnRH/GnRH (n = 155), respectively. In conclusion, estradiol replacement by GnRH in TAI protocols does not affect LF diameter, estrus expression and pregnancy rate in suckling Bos taurus beef cows.

南美的定时人工授精（TAI）方案主要基于黄体酮（P4）和雌二醇（E2）。考虑到对E2使用的限制，本研究旨在评估用GnRH替代E2的效果。对产乳母牛（n = 455）进行预同步，于妊娠第0天给予P4（1 g）阴道内装置（IVD）。在第0天，对照组奶牛给予2 mg苯甲酸雌二醇IM， GnRH/GnRH组和GnRH/EC组奶牛给予20 µg醋酸布丝林IM。在IVD移除（D7）时，所有奶牛接受500 µg氯前列醇钠IM， 300IU eCG IM和尾漆。此时，对照组和GnRH/EC组奶牛接受1 mg雌二醇（EC） IM。所有奶牛在第9天（IVD移除后48 h）进行TAI，仅GnRH/GnRH组给予10 µg醋酸布丝林IM。之间没有区别观察控制(n = 85),促/ EC (n = 82)和促性腺激性腺素释放素(n = 84)/关于最大卵泡(低频)直径D7(7.3 ± 0.2,7.6 ±  0.3和7.6±0.3  mm,分别;P = 0.75)和百分比的CL D7(87.1, 80.5和81.0 %。分别; = 0.37页)。D7 - LF直径的显著影响(P

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引用次数: 0

Transient receptor potential channel 4 (TRPC4) in boar sperm: Immunolocalization and functional analysis using the specific inhibitor ML204 猪精子瞬时受体电位通道4 (TRPC4)：特异性抑制剂ML204的免疫定位和功能分析

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-24 DOI: 10.1016/j.anireprosci.2025.108049

Eduardo de Mercado , Sonia Sánchez-Rodríguez , Adrián Martín-San Juan , Helena Nieto-Cristóbal , María José Martinez-Alborcia , Alejandro Vicente-Carrillo , Manuel Álvarez-Rodríguez

The TRPC4 channel is related to the movement of calcium within cells, and therefore possibly to sperm motility, capacitation and the acrosome reaction. This study investigated the presence and localization of TRPC4 channels in frozen-thawed boar sperm by direct immunofluorescence. In addition, different concentrations of the specific inhibitor ML204 were tested to evaluate its effect on post-thaw sperm motility and multiple sperm quality parameters assessed by flow cytometry, under conditions that promote motility recovery, such as caffeine supplementation or incubation in a basic extender. Our results confirmed that the presence of TRPC4 in boar sperm is independent of the use of ML204 and located in the midpiece and post-acrosomal regions. At the highest concentrations (22 µM), channel inhibition with ML204 caused a significant reduction in total and progressive motility, as well as in mitochondrial membrane potential, without a clear detrimental effect on the measured cytometry parameters. The loss of motility was exacerbated under co-incubation conditions with caffeine (2 mM), possibly due to an imbalance between calcium influx and demand via the cAMP/PKA pathway activated by this methylxanthine. Our results, however, when the thawing medium had a basic pH (8.2), motility was comparable to the control, likely because other pH-dependent channels compensated for the reduced calcium and cation influx. In conclusion, the TRPC4 channel is present in porcine sperm and appears to play an important role in regulating motility and maintaining mitochondrial membrane potential.

TRPC4通道与细胞内钙的运动有关，因此可能与精子运动、获能和顶体反应有关。本研究利用直接免疫荧光法研究了冻融猪精子中TRPC4通道的存在和定位。此外，在促进精子运动恢复的条件下，如补充咖啡因或在碱性扩展剂中孵育，我们测试了不同浓度的特异性抑制剂ML204，以评估其对解冻后精子活力的影响，并通过流细胞术评估了多个精子质量参数。我们的研究结果证实，TRPC4在野猪精子中的存在与ML204的使用无关，并且位于顶体中部和后区域。在最高浓度（22 µM）下，ML204通道抑制导致总运动和进行性运动以及线粒体膜电位显著降低，但对测量的细胞术参数没有明显的有害影响。在与咖啡因（2 mM）共孵育条件下，运动性丧失加剧，可能是由于甲基黄嘌呤激活的cAMP/PKA途径导致钙流入和需求之间的不平衡。然而，我们的研究结果表明，当解冻介质具有基本pH值（8.2）时，运动性与对照相当，可能是因为其他pH依赖性通道补偿了减少的钙和阳离子流入。综上所述，TRPC4通道存在于猪精子中，并在调节线粒体运动和维持线粒体膜电位方面发挥重要作用。

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引用次数: 0

Uterine proteomic and cytokine profiling show that Streptococcus equi subsp. zooepidemicus induces immune–metabolic dysregulation and may predispose mares to endometrosis 子宫蛋白质组学和细胞因子分析显示马链球菌亚种。动物流行病诱导免疫代谢失调，并可能使母马易患子宫内膜异位症

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-24 DOI: 10.1016/j.anireprosci.2025.108048

E. Da Silva-Álvarez , F.E. Martín-Cano , JM Alonso , L. Becerro-Rey , C. Zabalo-Palomo , MC Gil , F.J. Peña , C. Ortega-Ferrusola

This study aims to characterize and compare the uterine fluid proteome and cytokine profile of reproductively healthy mares (HM; n = 15) and mares with Streptococcus equi subsp. zooepidemicus (S. zooepidemicus)-associated endometritis (SAE; n = 9). Uterine flush samples collected during the follicular phase were analyzed by shotgun proteomics and multiplex cytokine assays. Equine proteins were mapped to human orthologs, and enrichment and network analyses were performed using g:Profiler, Metascape, ToppGene, and STRING/MCODE. SAE mares showed higher levels of IL-1α, IL-1β, GRO, IL12p70, and Eotaxin, and exclusive detection of IL-6, IL-8, IP10, IL-10 and TNFα, consistent with a proinflammatory and profibrotic environment. Proteomic analysis identified 67 differentially expressed proteins, indicating activation of innate immunity, metabolic reprogramming, and tissue remodeling. Upregulated proteins included neutrophil effectors linked to NET formation, acute-phase proteins, glycolytic enzymes, and chromatin-associated histones, whereas anti-inflammatory, antioxidant, or contractility-related proteins were reduced (SPI2, SOD3, ALDH1A1, CALM1). Enrichment and network analyses highlighted neutrophil degranulation/NETosis, glycolysis–gluconeogénesis, extracellular vesicle activity, and actin–cytoskeleton regulation, indicating immune–metabolic reprogramming of the uterine environment. Age-related decline in immune regulation may further exacerbate inflammatory and fibrotic responses in infected mares. The findings suggest that S. zooepidemicus infection induces a persistent neutrophil-driven inflammatory state and oxidative stress that compromise uterine clearance and may predispose mares to fibrosis. Sixteen candidate biomarkers related to chromatin remodeling, antimicrobial defense, and metabolic regulation were identified, providing potential diagnostic value. Therapeutic strategies targeting dysregulated inflammation and NETosis, alongside antimicrobial therapy, may improve fertility outcomes in mares affected by infectious endometritis

本研究旨在描述和比较生殖健康母马（HM; n = 15）和感染马链球菌亚种的母马子宫液蛋白质组和细胞因子谱。动物流行病(S. zooepidemic)-相关性子宫内膜炎（SAE; n = 9）。采用散弹枪蛋白质组学和多重细胞因子法对卵泡期子宫潮红标本进行分析。马蛋白被定位到人类同源物，并使用g:Profiler、metscape、ToppGene和STRING/MCODE进行富集和网络分析。SAE母马IL-1α、IL-1β、GRO、il - 12p70、Eotaxin水平均较高，IL-6、IL-8、IP10、IL-10、TNFα均未检出，与促炎、促纤维化环境一致。蛋白质组学分析鉴定出67种差异表达蛋白，表明先天免疫激活、代谢重编程和组织重塑。上调的蛋白包括与NET形成相关的中性粒细胞效应蛋白、急性期蛋白、糖酵解酶和染色质相关组蛋白，而抗炎、抗氧化或收缩性相关蛋白则减少（SPI2、SOD3、ALDH1A1、CALM1）。富集和网络分析强调了中性粒细胞脱粒/NETosis，糖酵解-糖异糖变性，细胞外囊泡活性和肌动蛋白-细胞骨架调节，表明子宫环境的免疫代谢重编程。年龄相关的免疫调节下降可能进一步加剧感染母马的炎症和纤维化反应。研究结果表明，动物流行性链球菌感染诱导持续的中性粒细胞驱动的炎症状态和氧化应激，从而损害子宫清除，并可能使母马易发生纤维化。鉴定出16个与染色质重塑、抗菌防御和代谢调节相关的候选生物标志物，提供潜在的诊断价值。针对炎症失调和NETosis的治疗策略，以及抗菌治疗，可能改善受感染性子宫内膜炎影响的母马的生育结果

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引用次数: 0

Impact of dendrobium nobile supplementation on antioxidant activity and egg-laying performance in late-laying hens 添加石斛对产蛋鸡抗氧化活性和产蛋性能的影响

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-22 DOI: 10.1016/j.anireprosci.2025.108046

Gang Feng , Yushi Shi , Depeng Zhao , Xia Long , Qisong Tan , Jinlin Yang , BeiDa Zhou , Hui Li

The purpose of this study was to investigate the impact of Dendrobium nobile on the antioxidant capacity and laying performance of hens during the late laying period. A total of 90 recessive white hens (56 weeks old) were divided into Control group (basal diet) and DNL group (basal diet +0.25 % dendrobium powder). Evaluate the impact of Dendrobium nobile on biochemical indicators in serum, ovarian mRNA expression levels, and ovarian metabolomic patterns in hens, and conduct a correlation analysis. Supplementing the diet with Dendrobium nobile powder enhanced the serum levels of follicle stimulating hormone and the expression of the egg-laying-related gene FSHR. This supplementation led to increased laying rates and egg production in hens during the late laying period, and elevated activity levels of catalase, glutathione peroxidase, and total superoxide dismutase. Further, mRNA expression levels of the Nrf2 signaling pathway genes were upregulated, ultimately mitigating the effects of oxidative stress on the ovary. Metabolomic analysis identified 94 metabolites with significant differences, including 55 upregulated and 39 downregulated molecules. Significant differences in metabolites were predominantly observed in pathways related to fatty acid degradation, aminoacyl-tRNA biosynthesis, and unsaturated fatty acid biosynthesis. These pathways appear to be the primary mechanism through which Dendrobium nobile enhances ovarian antioxidant capacity and egg-laying traits at the metabolic level. Supplementing the diet with Dendrobium nobile enhances antioxidant activity and laying performance in hens during the late laying period.

本试验旨在研究石斛对蛋鸡产蛋后期抗氧化能力和产蛋性能的影响。试验选用90只56周龄的隐性白鸡，分为对照组（基础饲粮）和DNL组（基础饲粮+0.25 %石斛粉）。评价石斛对蛋鸡血清生化指标、卵巢mRNA表达水平和卵巢代谢组学模式的影响，并进行相关分析。饲粮中添加石斛粉可提高血清促卵泡激素水平和产蛋相关基因FSHR的表达。在蛋鸡产蛋后期，添加该添加剂可提高产蛋率和产蛋量，并提高过氧化氢酶、谷胱甘肽过氧化物酶和总超氧化物歧化酶的活性水平。此外，Nrf2信号通路基因的mRNA表达水平上调，最终减轻了氧化应激对卵巢的影响。代谢组学分析鉴定出94种差异显著的代谢物，其中55种上调，39种下调。代谢产物的显著差异主要发生在脂肪酸降解、氨基酰基trna生物合成和不饱和脂肪酸生物合成相关的途径中。这些途径可能是石斛在代谢水平上提高卵巢抗氧化能力和产蛋性状的主要机制。饲粮中添加石斛可提高蛋鸡产蛋后期的抗氧化活性和产蛋性能。

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引用次数: 0