Animal Reproduction Science最新文献_第6页

Older yet more attractive: Multiparous ewes are preferentially courted, and elicit more mountings and ejaculations than nulliparous ewes in group breeding 年龄更大，但更有吸引力：在群体繁殖中，产多胎的母羊比没有产过的母羊更容易交配和射精

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-21 DOI: 10.1016/j.anireprosci.2025.108047

Gustavo Dias , Rodolfo Ungerfeld , Aline Freitas-de-Melo , Adroaldo Zanella

High ewe density in group breeding enhances ram selectivity and favors the most attractive ewes. This study aimed to compare the sexual attractiveness of multiparous and nulliparous ewes. We hypothesize that multiparous ewes will be ranked as more attractive. Forty-two ewes, 21 multiparous and 21 nulliparous, Santa Ines × Dorper in estrus were tested. Each ewe was observed for five minutes, and behaviour was recorded from live and video observations. After each 5-minute test, the ewe courted for the longest time was removed and ranked as most attractive (rank 1), and this procedure continued until all six ewes in a group were ranked (1 = most to 6 = least attractive). Ewe ranking, ram behavior, and the odds of mounting acceptance were analyzed using the Friedman test, generalized mixed-effects models, and mixed-effects logistic regression, respectively. Mean attractiveness ranks were higher for multiparous than nulliparous ewes (2 ± 0.17 vs. 5 ± 0.17, P = 0.008). Rams also exhibited longer mean mounting duration (2.46 ± 0.91 s vs. 1.59 ± 0.91 s, P = 0.045) and a higher number of ejaculations (0.90 ± 0.31 vs. 0.14 ± 0.31, P = 0.0015) with multiparous ewes. While odds of accepting mounts were similar between categories (odds ratio = 0.45; 95 % CI: 0.08–2.75; P = 0.369). All first-ranked ewes were multiparous, and last two ranks were nulliparous. These results indicate multiparous estrous ewes as more attractive to rams, suggesting separating breeding groups per parity may improve fertilization rates in nulliparous ewes.

在群体繁殖中，母羊密度高可以提高公羊的选择性，有利于最有吸引力的母羊。本研究旨在比较多产母羊和无产母羊的性吸引力。我们假设，产多胎的母羊会被认为更有吸引力。选取发情期Santa Ines × 杜珀母羊42只母羊，分别为21只多产和21只无产母羊。研究人员对每只母羊进行了五分钟的观察，并通过现场观察和视频观察记录了它们的行为。在每5分钟的测试后，求偶时间最长的母羊被移除，并被评为最具吸引力（排名1），这一过程一直持续到一组中的所有六只母羊被排名（1 =最具吸引力到6 =最不具吸引力）。母羊排名、公羊行为和被接受的几率分别使用弗里德曼检验、广义混合效应模型和混合效应逻辑回归进行分析。多产母羊的平均吸引力等级高于无产母羊（2 ± 0.17比5 ± 0.17,P = 0.008）。公羊也表现出长意味着越来越多的时间(2.46 ±0.91  年代与1.59 ±0.91  s, P = 0.045)和更多的随笔( 0.90±0.31 vs 0.14  ± 0.31,P = 0.0015)和多产的母羊。而接受坐骑的几率在不同类别之间相似（优势比= 0.45;95 % CI: 0.08-2.75; P = 0.369）。排名第一的母羊均为多胎母羊，排名最后两名的母羊均为无胎母羊。这些结果表明，多胎母羊对公羊更有吸引力，表明每胎分开繁殖组可以提高无胎母羊的受精率。

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引用次数: 0

Proteomics reveals substantial differences between in vitro matured abattoir-derived and in vivo matured oocytes in cattle 蛋白质组学揭示了牛体外成熟的屠宰场衍生的卵母细胞和体内成熟的卵母细胞之间的实质性差异。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-17 DOI: 10.1016/j.anireprosci.2025.108045

Rebecca Herbicht , Bruno Joaquin Paredes Osorio , Claudia Klein

In vitro production (IVP) of bovine embryos still has its limitations, such as low blastocyst rate and lower embryo quality, resulting in lower pregnancy rates following transfer compared to in vivo produced embryos. Transcriptional studies comparing both types of embryos mainly utilized oocytes retrieved from slaughterhouse material for IVP, thereby introducing the possibility, that differences between IVP and in vivo embryos are in part attributable to differing sources of oocytes. The aim of the present study was therefore to compare the proteome of oocytes retrieved from slaughterhouse material, with and without a period of in vitro maturation, and in vivo matured oocytes obtained from superovulated donor cattle. For each group the protein pattern of four technical replicates containing ten oocytes each were analyzed via SWATH™-MS. In total, 1208 proteins were detected, 160 of which were differentially abundant in at least one group comparisons. Clustering analysis revealed that oocyte maturation environment is the primary driver of molecular difference, with the in vitro matured oocytes exhibiting a highly distinct proteome compared to both the in vivo matured and immature oocytes. In vitro matured oocytes were characterized by an overall enhanced translational activity affecting mainly proteins involved in energy metabolism, transcription and translation, and oocyte activation. An organized regulation of key pathways was observed during oocyte maturation in vivo, e.g. activation of autophagy and apoptosis pathways, while changes in protein abundance in vitro seem to be undirected. This deregulation of the proteome in in vitro matured oocytes likely negatively affect subsequent developmental events.

牛胚胎的体外生产（IVP）仍有其局限性，如囊胚率低，胚胎质量较低，导致移植后的妊娠率低于体内生产的胚胎。比较两种胚胎类型的转录研究主要利用从屠宰场材料中提取的卵母细胞进行体外受精，从而引入了一种可能性，即体外受精和体内胚胎之间的差异部分归因于卵母细胞来源的不同。因此，本研究的目的是比较从屠宰场材料中获得的卵母细胞的蛋白质组，有和没有经过一段时间的体外成熟，以及从超排卵供体牛中获得的体内成熟卵母细胞。通过SWATH™-MS分析每组包含10个卵母细胞的4个技术重复的蛋白质模式。总共检测到1208种蛋白质，其中160种在至少一个组比较中差异丰富。聚类分析显示，卵母细胞成熟环境是分子差异的主要驱动因素，体外成熟卵母细胞与体内成熟和未成熟卵母细胞相比，表现出高度不同的蛋白质组。体外成熟卵母细胞的特点是翻译活性总体增强，主要影响涉及能量代谢、转录和翻译以及卵母细胞活化的蛋白质。在体内观察到卵母细胞成熟过程中关键通路的有组织调节，如自噬和凋亡通路的激活，而体外蛋白质丰度的变化似乎是无定向的。这种体外成熟卵母细胞中蛋白质组的失调可能对随后的发育事件产生负面影响。

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引用次数: 0

Cultivation of all-male large yellow croaker (Larimichthys crocea) and evaluation of its temperature tolerance and hypoxia tolerance 全雄大黄鱼的培育及其耐温、耐缺氧评价。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-15 DOI: 10.1016/j.anireprosci.2025.108042

Yang Liu , Jie Ding , Tianyi Lan , Li Wang , Weiliang Shen , Xiongfei Wu , Daojun Tang , Junquan Zhu

Mono-sex cultures of all-male large yellow croaker (Larimichthys crocea) offers clear advantages for boosting economic returns in aquaculture. The purpose of this study was to breed all-male large yellow croaker. To achieve this goal, we first successfully induced sex reversal of YY supermale fish to produce YY pseudo-female through adding 17β-estradiol (E₂, 120 mg/kg). These YY pseudo-female were then crossed with YY supermale fish to establish a pure-bred YY supermale fish population. Subsequently, the YY supermale fish were hybridized with female fish to produce all-male fish. During the breeding process, YY pseudo-female, YY supermale and XY all-male fish were identified by sex-specific molecular markers and gonad histological observation. The results showed that in the experiment of inducing sex reversal of YY supermale fish. The survival rate of YY pseudo-female fish was 63.4 %, and the hatching rate of its offspring pure-bred YY supermale fish was 68.06 %. There was no significant difference in growth, testes development stage, sex steroid hormone levels and the expression of testis development-related genes levels between pure-bred YY supermale and normal male fish. In the temperature tolerance assay, the all-male group exhibited significantly higher survival rates than the control under both high- and low-temperature stress. In the hypoxia tolerance experiment, the respiratory oxygen consumption rate of all-male fish decreased more with the decrease of dissolved oxygen in water, and the asphyxiation point was lower. It shows that the all-male fish is more adaptable to the adverse environment. In summary, it is feasible to obtain a pure-bred YY supermale fish population by inducing YY supermale fish sex reversal. On this basis, the all-male fish population can be produced, and the stress resistance advantages of all-male fish have considerable application potential for the aquaculture of large yellow croakers.

全雄大黄鱼单性养殖在提高养殖经济效益方面具有明显的优势。本研究的目的是培育全雄性大黄鱼。为了实现这一目标，我们首先通过添加17β-雌二醇（E2, 120 mg/kg）成功诱导YY超雄鱼性别逆转产生YY伪雌鱼。然后将这些YY假雌鱼与YY超雄鱼杂交，建立一个纯种YY超雄鱼种群。随后，将YY超雄鱼与雌鱼杂交，产生全雄鱼。在繁殖过程中，通过性别特异性分子标记和性腺组织学观察，鉴定了YY条伪雌鱼、YY条超雄鱼和XY条全雄鱼。结果表明，在诱导YY超雄鱼性别逆转的实验中。YY假雌鱼的成活率为63.4 %，其后代纯种YY超雄鱼的孵化率为68.06 %。纯种YY超雄鱼与正常雄鱼的生长、睾丸发育阶段、性类固醇激素水平及睾丸发育相关基因表达水平均无显著差异。在耐温试验中，在高温和低温胁迫下，全雄性组的成活率均显著高于对照组。在耐缺氧实验中，全雄鱼的呼吸耗氧率随着水中溶解氧的减少而下降较多，窒息点较低。这表明全雄鱼对不利环境的适应能力更强。综上所述，通过诱导YY超雄鱼性别反转获得纯种YY超雄鱼种群是可行的。在此基础上可以生产出全雄鱼种群，全雄鱼的抗逆性优势在大黄鱼养殖中具有相当大的应用潜力。

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引用次数: 0

Lasia spinosa supplementation improves semen quality and sperm cryosurvival in subfertile beef bulls: An exploratory testicular transcriptomic analysis 补充棘叶草可提高低生育能力肉牛的精液质量和精子冷冻存活率：一项探索性睾丸转录组学分析。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-15 DOI: 10.1016/j.anireprosci.2025.108043

Supitcha Kaewma , Nitira Anakkul , Ratree Jintana , Junpen Suwimonteerabutr , Saritvich Panyaboriban , Kamolporn Dhanarun , Theerawat Tharasanit , Takeshige Otoi , Mongkol Techakumphu , Theerawat Swangchan-Uthai

Heat stress can impair semen quality in imported bull sires. Recent research has shown the potential of Lasia spinosa Thw. (LST) to improve semen quality in boars, so we aimed to investigate the effect of LST supplementation on semen quality in subfertile bulls, focusing on semen characteristics in both fresh and frozen–thawed ejaculates as well as the transcriptomic changes in testicular tissues. Six imported beef bull sires presenting poor semen quality for > 5 months at the Bull Semen Production Center were included in the experiment. They received daily supplementation with LST (5 g/100 kg body weight). Semen and serum testosterone evaluations were performed weekly for 24 weeks. For histologic and transcriptomic evaluations, testicular biopsies were performed on three LST-supplemented bulls, pre- and post-supplementation, and three control bulls. We observed significant improvements in movement kinetics, viability, acrosome integrity, membrane integrity, and sperm head morphology in LST-supplemented bulls. Frozen–thawed semen presented significant enhancements in parameters such as motility and mitochondrial activity. Serum testosterone significantly increased after supplementation, correlating significantly with sperm motility and mitochondrial activity. Transcriptomic analysis identified 16 downregulated genes in subfertile bulls; LST supplementation induced upregulation of 11 genes related to germ cell structure, spermatogenesis, and sperm quality, including CNFN, KRT17, KRT1, KRT19, DSC3, KLK8, SPRR4, SPRR2E-like, Serpin B4-like, and WFDC18-like. Our findings highlight the potential of LST supplementation as a promising intervention for enhancing reproductive performance in subfertile bulls.

热应激会影响进口公牛的精液质量。最近的研究表明，刺叶草的潜力。因此，本研究旨在研究添加LST对低育公牛精液质量的影响，重点研究新鲜和冻融射精的精液特征以及睾丸组织的转录组变化。以公牛精液生产中心5个月来精液质量较差的6头进口牛肉为对象进行了实验。他们每天补充LST（5 g/100 kg体重）。每周进行精液和血清睾酮评估，持续24周。为了进行组织学和转录组学评估，对三只补充lst的公牛、补充前和补充后以及三只对照公牛进行了睾丸活检。我们观察到补充lst的公牛在运动动力学、活力、顶体完整性、膜完整性和精子头形态方面有显著改善。冻融后的精液在运动性和线粒体活性等参数上有显著增强。补充后血清睾酮显著升高，与精子活力和线粒体活性显著相关。转录组学分析鉴定出16个低受精公牛的下调基因；LST补充诱导了11个与生殖细胞结构、精子发生和精子质量相关的基因上调，包括CNFN、KRT17、KRT1、KRT19、DSC3、KLK8、SPRR4、sprr2 -like、Serpin B4-like和WFDC18-like。我们的研究结果强调了LST补充作为一种有希望的干预措施的潜力，以提高不育公牛的生殖性能。

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引用次数: 0

Chitosan-based nanoconjugated hormone delivery boosts reproductive efficiency in male Labeo rohita 壳聚糖纳米偶联激素的传递提高了雄性大腹小腹的生殖效率。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-10 DOI: 10.1016/j.anireprosci.2025.108041

Mohd Ashraf Malik , Subodh Gupta , Tincy Varghese , Shrinivas Jahageerdar , Sunil Kumar Nayak , Dhalongsaih Reang

Hormone-induced breeding is pivotal in freshwater aquaculture, yet conventional gonadotropin-releasing hormone analogues (GnRH-a) are limited by their rapid degradation and cause stress associated with high-dose injections. Chitosan nanocarriers offer improved hormone stability and delivery. This study evaluated the reproductive efficacy of chitosan nanoconjugated salmon GnRH-a (ChN-sGnRH-a), alone or with domperidone, in male Labeo rohita. Six treatments were tested: Cn (Negative control, nanocarrier control), Cp (Positive control, commercial GnRH-a: Gonopro-FH® at 0.1 ml/kg), T1 (ChN-sGnRH-a at full dose, 0.1 ml/kg), T2 (T1 + domperidone 10 mg/ml), T3 (ChN-sGnRH-a at half dose, 0.05 ml/kg), and T4 (T3 + domperidone 10 mg/ml). T2 resulted in significantly higher (P < 0.05) relative milt volume (8.11 ± 0.47 ml/kg), sperm motility (85.7 ± 1.8 %), and sperm count (3.97 ± 0.05 × 10 ¹⁰/ml) compared to Cp. Serum testosterone (5.2 ± 0.3 ng/ml), 11-ketotestosterone (4.8 ± 0.2 ng/ml), and 17α,20β-dihydroxyprogesterone (2.1 ± 0.1 ng/ml) peaked at 12 h post-injection in T2, sustaining elevated levels over 18 h. T2 also showed significantly (P < 0.05) higher expression of fshβ, lhβ, fshr, and lhr genes, along with advanced testicular histoarchitecture marked by abundant spermatozoa and active leydig/sertoli cells. Principal component analysis revealed that T2, T4 and Cp grouped separately with more than 97 % variance covered by reproductive indices. Notably, T4 (half-dose) was comparable to Cp, suggesting efficacy at reduced hormone dosage. Overall, the findings demonstrate that full-dose ChN-sGnRH-a coadministered with domperidone is superior to commercial GnRH-a, and half-dose nanoconjugates provide equal efficacy, justifying a dose-reduction regime for sustainable, low-stress broodstock management of L. rohita.

激素诱导育种在淡水水产养殖中至关重要，但传统的促性腺激素释放激素类似物（GnRH-a）因其快速降解而受到限制，并且在大剂量注射时引起应激。壳聚糖纳米载体提高了激素的稳定性和输送能力。本研究评价了壳聚糖纳米偶联鲑鱼GnRH-a （ChN-sGnRH-a）单独或与多潘立酮联用在雄性罗氏大鲵体内的生殖效果。六个治疗进行了测试:Cn(负控制、nanocarrier控制),Cp(积极控制,商业GnRH-a: Gonopro-FH®0.1 毫升/公斤),T1 (ChN-sGnRH-a全剂量,0.1 毫升/公斤),T2 (T1 + domperidone 10毫克/毫升),T3 (ChN-sGnRH-a一半剂量,0.05 毫升/公斤),和T4 (T3 + domperidone 10毫克/毫升)。T2显著升高(P

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引用次数: 0

MiR-23a represses CELF1 to modulate buffalo granulosa cell apoptosis and steroidogenesis via the PI3K/AKT pathway MiR-23a通过PI3K/AKT通路抑制CELF1调节水牛颗粒细胞凋亡和类固醇生成。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-09 DOI: 10.1016/j.anireprosci.2025.108040

Haoxin Wang , Yun Feng , Xin Zhao , Penglei Shen , Deshun Shi , Fenghua Lu

MicroRNAs (miRNAs) are critical regulators of mammalian follicle development. Our previous work showed that miR-23a expression was significantly higher in buffalo oocytes at metaphase II (MII) than at the germinal vesicle (GV) stage, implying a role in oocyte maturation. Here, we investigated the function and mechanism of miR-23a in buffalo granulosa cells (GCs). GCs cultured in vitro were transfected with miR-23a mimics or inhibitor. The miR-23a mimic markedly increased apoptosis, reduced estradiol (E2) release, elevated progesterone (P4) secretion, and suppressed the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway, whereas the inhibitor exerted the opposite effects. Dual-luciferase and rescue assays revealed that miR-23a directly targets CUG triplet repeat-binding protein 1 (CELF1). Overexpression of CELF1 reversed the pro-apoptotic effect of miR-23a, restored proliferation, normalized E2 and P4 production, and re-activated PI3K/AKT signaling. Thus, miR-23a targeted CELF1 to inhibit the PI3K/AKT pathway, promoting apoptosis, suppressing proliferation and modulating steroidogenesis in buffalo GCs, thereby controlling follicular homeostasis.

MicroRNAs （miRNAs）是哺乳动物卵泡发育的关键调控因子。我们之前的研究表明，miR-23a在水牛卵母细胞II中期（MII）的表达明显高于生发囊泡（GV）阶段，这表明miR-23a在卵母细胞成熟中起作用。在这里，我们研究了miR-23a在水牛颗粒细胞（GCs）中的功能和机制。用miR-23a模拟物或抑制剂转染体外培养的GCs。miR-23a模拟物显著增加细胞凋亡，减少雌二醇（E2）释放，升高孕酮（P4）分泌，抑制磷酸肌醇3-激酶/蛋白激酶B （PI3K/AKT）通路，而抑制剂则发挥相反的作用。双荧光素酶和挽救实验显示，miR-23a直接靶向CUG三联体重复结合蛋白1 （CELF1）。CELF1的过表达逆转了miR-23a的促凋亡作用，恢复了细胞增殖，使E2和P4的产生正常化，并重新激活了PI3K/AKT信号。因此，miR-23a靶向CELF1抑制PI3K/AKT通路，促进水牛GCs细胞凋亡，抑制细胞增殖，调节甾体生成，从而控制卵泡内稳态。

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引用次数: 0

Nicotinamide mononucleotide supplement combined with prolonging maturation time improves the quality of oocytes cultured in vitro 补充烟酰胺单核苷酸联合延长成熟时间可提高体外培养卵母细胞的质量

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-08 DOI: 10.1016/j.anireprosci.2025.108039

Meiyun Lv , Xingrong Lu , Peng Qiu , Lei Wang , Yunchuan Zhang , Yanfei Deng , Deshun Shi , Chan Luo

Due to the limited in vitro culture duration, oocyte maturation efficiency in vitro is lower than in vivo. Nicotinamide mononucleotide (NMN), a precursor of NAD⁺ and a cofactor of Sirtuin deacetylases, exhibits significant antioxidant activity. This study aims to evaluate whether NMN supplementation combined with prolonging maturation time enhances cytoplasmic maturation and improves porcine oocyte quality. The blastocyst rate increased significantly after parthenogenetic activation when maturation time was extended from 44 h to 47 h or 50 h, peaking at 47 h. Prolonged maturation improved mitochondrial function and cortical granule distribution but induced ROS accumulation and apoptosis. Notably, supplementation with 100 μM NMN reversed these negative effects, significantly increasing the total number of blastocyst cells, reducing ROS levels, and upregulating cytoplasmic quality-related gene expression. In conclusion, extending maturation to 47 h with 100 μM NMN supplementation enhances oocyte maturation and embryonic developmental potential in vitro. These findings suggest that NMN, a naturally occurring nucleotide in food, may improve oocyte quality and offer insights for optimizing in vitro culture techniques as well as treating oocyte quality-related infertility.

由于体外培养时间有限，卵母细胞体外成熟效率低于体内成熟效率。烟酰胺单核苷酸（NMN）是NAD+的前体和Sirtuin脱乙酰酶的辅助因子，具有显著的抗氧化活性。本研究旨在评价添加NMN并延长成熟时间是否能促进细胞质成熟，提高猪卵母细胞质量。当成熟时间从44 h延长到47 h或50 h时，孤雌激活后囊胚率显著增加，在47 h达到峰值。成熟时间延长可改善线粒体功能和皮质颗粒分布，但可诱导ROS积累和细胞凋亡。值得注意的是，添加100 μM NMN可以逆转这些负面影响，显著增加囊胚细胞总数，降低ROS水平，上调细胞质质量相关基因表达。综上所述，添加100 μM NMN可将成熟时间延长至47 h，提高卵母细胞体外成熟和胚胎发育潜力。这些发现表明，食品中天然存在的核苷酸NMN可能改善卵母细胞质量，并为优化体外培养技术以及治疗与卵母细胞质量相关的不孕症提供见解。

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引用次数: 0

Effects of dietary supplementation with gallnut tannic acid in early pregnancy on reproductive performance and gut microbiota of sows 妊娠早期饲粮中添加五倍子单宁酸对母猪繁殖性能和肠道菌群的影响

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-06 DOI: 10.1016/j.anireprosci.2025.108028

Jie Wang , Guangling Hu , Qingwu Shi , Zhijun Luo , Zhenqing Yang , Caizai Zhang , Yiyu Zhang , Xiaoli Shi , Zheng Ao

The survival of early embryos during the first trimester is closely associated with sow reproductive performance. Embryo development and implantation in this period are accompanied by oxidative stress and inflammation. Gallnut tannic acid (GTA) has demonstrated strong antioxidant and anti-inflammatory properties; however, its effects on sow reproductive performance remain poorly defined. Here, we investigated the effects of dietary GTA supplementation during early gestation (days 7–30) on sow reproductive performance, gut microbiota, and underlying mechanisms. A total of 100 sows (Landrace × Large White) were allocated to four groups (n = 25 per group): a basal control diet (CON) or the basal diet supplemented with 0.05 % (I), 0.1 % (II), or 0.2 % (III) GTA. GTA supplementation tended to increase average litter size (P = 0.068), with the 0.1 % group (II) achieving the highest value (17.05 piglets per litter). GTA enhanced systemic antioxidant capacity by increasing serum total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels, whereas malondialdehyde (MDA). It also significantly reduced serum IL-6 concentrations levels (P < 0.05). In vitro, 6 μM tannic acid (TA) promoted trophoblast cell proliferation and upregulated the expression of implantation-related genes (PLET1, SPP1), antioxidant activity (CAT, CuZnSOD, MnSOD), an angiogenesis marker (VEGFR1), and the proliferation marker PCNA, while modulating inflammatory gene expression in porcine trophoblast (pTr2) cells (P < 0.05). Gut microbiota analysis revealed dose-dependent alterations, with the 0.2 % GTA group (III) showing the most pronounced compositional shifts at both phylum and family levels. In conclusion, dietary GTA supplementation during early gestation may improve sow reproductive performance by enhancing antioxidant capacity, regulating inflammatory homeostasis, and upregulating implantation-related gene expression. The optimal dietary level appears to be approximately 0.1 % GTA.

妊娠早期胚胎的存活率与母猪的繁殖性能密切相关。这一时期的胚胎发育和着床伴随着氧化应激和炎症反应。五倍子单宁酸（GTA）具有很强的抗氧化和抗炎特性；然而，其对母猪繁殖性能的影响仍不明确。本研究旨在研究妊娠早期（第7-30天）饲粮中添加GTA对母猪繁殖性能、肠道微生物群的影响及其机制。试验选用100头长白×大母猪，分为4组（n = 25头/组）：基础对照饲粮（CON）或基础饲粮中添加0.05 % (I)、0.1 % （II）、0.2 % (III) GTA。饲粮中添加GTA有增加平均窝产仔数的趋势（P = 0.068），其中添加0.1% %组（II）达到最高水平（17.05头/窝）。GTA通过提高血清总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）水平来增强全身抗氧化能力，而丙二醛（MDA）则相反。血清IL-6浓度也显著降低（P <； 0.05）。在体外实验中，6 μM单宁酸（TA）促进滋养层细胞增殖，上调着床相关基因（PLET1、SPP1）、抗氧化活性（CAT、CuZnSOD、MnSOD）、血管生成标志物（VEGFR1）和增殖标志物PCNA的表达，同时调节猪滋养层细胞（pTr2）炎症基因的表达（P <； 0.05）。肠道菌群分析显示出剂量依赖性改变，0.2 % GTA组（III）在门和科水平上都显示出最明显的组成变化。综上所述，妊娠早期饲粮中添加GTA可能通过增强抗氧化能力、调节炎症稳态和上调植入相关基因表达来改善母猪的繁殖性能。最佳日粮水平约为0.1 % GTA。

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引用次数: 0

Characteristics of collagen in ovarian extracellular matrix and its relationship with the development of primordial follicles up to ovulation 卵巢细胞外基质中胶原蛋白的特征及其与原始卵泡发育直至排卵的关系。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-03 DOI: 10.1016/j.anireprosci.2025.108029

Emanoel da S. Félix, José R.V. Silva

The extracellular matrix (ECM) is a dynamic network of proteins and biomolecules that performs essential structural and signaling functions in mammalian ovarian physiology. In addition to modulating processes such as cell adhesion, migration, and differentiation, the ECM is actively involved in regulating tissue stiffness, a physical property directly involved in follicle activation and development. Among its components, collagen fibrils stand out as determinants in maintaining ovarian architecture and remodeling the follicular microenvironment throughout folliculogenesis. Evidence from in vivo and in vitro models shows that changes in the composition, organization, and distribution of collagen fibrils, particularly types I, III, IV, and VI, influence the transition from primordial follicles to growth stages and are involved in the intense remodeling that occurs during ovulation and reproductive senescence. In this review, we discuss the central role of collagen fibrils in regulating ovarian cortex stiffness and its relationship to follicular growth, highlighting cellular and molecular mechanisms associated with ECM remodeling in different species and physiological contexts.

细胞外基质（ECM）是一个由蛋白质和生物分子组成的动态网络，在哺乳动物卵巢生理中具有重要的结构和信号功能。除了调节细胞粘附、迁移和分化等过程外，ECM还积极参与调节组织刚度，这是一种直接参与卵泡激活和发育的物理特性。在其组成部分中，胶原原纤维作为维持卵巢结构和重塑卵泡微环境的决定因素在卵泡发生过程中脱颖而出。来自体内和体外模型的证据表明，胶原原纤维的组成、组织和分布的变化，特别是I、III、IV和VI型胶原原纤维的变化，影响了从原始卵泡到生长阶段的转变，并参与了排卵和生殖衰老期间发生的强烈重塑。在这篇综述中，我们讨论了胶原原纤维在调节卵巢皮质硬度中的核心作用及其与卵泡生长的关系，强调了不同物种和生理背景下与ECM重塑相关的细胞和分子机制。

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引用次数: 0

Levonorgestrel-induced sex reversal in mandarin fish (Siniperca chuatsi): An effective protocol for all-female production and its aquaculture applications 左炔诺孕酮诱导鳜鱼性别逆转：全雌生产及其水产养殖应用的有效方案。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-11-03 DOI: 10.1016/j.anireprosci.2025.108030

Shiyan Liu , Jinmeng Teng , Suhan Peng , Shuang Liu , Jiaqi Lin , Long Chen , Ju Hu , Haoran Lin , Caiyun Sun , Shuisheng Li , Songlin Chen , Chong Han , Yong Zhang

This study aimed to develop and evaluate an effective protocol for producing all-female populations of mandarin fish (Siniperca chuatsi) through levonorgestrel (LNG)-induced sex reversal of genetic females. During the critical sex differentiation window (15–60 days post hatching), administration of 100 mg/kg LNG through prey-mediated delivery induced 100 % sex reversal of genetic females into functional neo-males, as confirmed by histological and molecular analyses. The neo-males exhibited normal testicular development, complete spermatogenesis, and reproductive competence comparable to XY males. In vivo gene expression analysis revealed that the masculinized state induced by LNG was characterized by the significant upregulation of male-biased genes (dmrt1, gsdf, amh, sox9, and arβ) and the suppression of female-biased genes (foxl2 and cyp19a1a). Furthermore, our in vitro results suggested that LNG could act directly on ovarian cells to induce a similar molecular shift, also modulating reproductive regulation-related genes (fshr and lhr). Crossbreeding experiments demonstrated that the all-female progeny derived from LNG-induced neo-males showed 21 % greater growth performance by 240 dph compared to mixed-sex populations. These findings establish a highly efficient and practical protocol for the production of all-female mandarin fish, demonstrating a valuable application of reproductive technology to enhance aquaculture productivity.

本研究旨在通过左炔诺孕酮（LNG）诱导的遗传雌性性别逆转，建立和评估鳜鱼（sininiperca chuatsi）全雌种群的有效方案。组织学和分子分析证实，在关键的性别分化窗口（孵化后15-60天），通过捕食介导的方式给药100 mg/kg LNG诱导了100% %的遗传雌性向功能性新雄性的性别逆转。新雄性表现出正常的睾丸发育、完整的精子发生和与XY雄性相当的生殖能力。体内基因表达分析显示，LNG诱导的雄性化状态的特征是雄性偏向基因（dmrt1、gsdf、amh、sox9和arβ）的显著上调和雌性偏向基因（foxl2和cyp19a1a）的抑制。此外，我们的体外实验结果表明，LNG可以直接作用于卵巢细胞，诱导类似的分子转移，同时调节生殖调节相关基因（fshr和lhr）。杂交实验表明，由lng诱导的新雄性获得的全雌性后代比混合性别群体的生长性能提高了21% %，提高了240 dph。这些发现为全雌鳜鱼的生产建立了一个高效和实用的方案，展示了生殖技术在提高水产养殖生产力方面的有价值的应用。

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引用次数: 0