We have examined the transcriptional activities of the mouse oocyte genome during follicle growth in order to obtain information on the stages at which the synthesis of particular RNA species becomes important. The effects of gonadotrophins on these patterns of transcription have also been investigated. RNA synthesis in oocytes was measured at different stages of growth using a cytochemical application of the standard biochemical assay for DNA-dependent RNA polymerase. The presence of endogenous nucleolar and nucleoplasmic polymerases was detected by autoradiography and their relative activities were quantitated by grain counts. Both activities were relatively low in oocytes of the resting pool of small follicles but increased as growth commenced, reaching a peak as the oocytes approached maximum size. Thereafter RNA polymerase activities declined rapidly and were very low in the oocytes of large, antral follicles. Administration of PMSG to mice had no significant effect on the polymerases of oocytes of small follicles or during the early stages of follicles growth when compared with saline-treated controls. However, in oocytes which had attained maximum size, a statistically significant increase in nucleolar labelling was detected after PMSG treatment. The elevated levels of RNA polymerase activity were confined to oocytes within this growth compartment. These observations are discussed in relation to the way the production of gene transcripts is regulated during the growth of the oocyte, both with respect to the storage of informational molecules and the development of meiotic maturation competence.
{"title":"Patterns of gene activity during ovum formation in the mouse","authors":"G. Moore, S. Lintern-moore","doi":"10.1051/RND:19790902","DOIUrl":"https://doi.org/10.1051/RND:19790902","url":null,"abstract":"We have examined the transcriptional activities of the mouse oocyte genome during follicle growth in order to obtain information on the stages at which the synthesis of particular RNA species becomes important. The effects of gonadotrophins on these patterns of transcription have also been investigated. RNA synthesis in oocytes was measured at different stages of growth using a cytochemical application of the standard biochemical assay for DNA-dependent RNA polymerase. The presence of endogenous nucleolar and nucleoplasmic polymerases was detected by autoradiography and their relative activities were quantitated by grain counts. Both activities were relatively low in oocytes of the resting pool of small follicles but increased as growth commenced, reaching a peak as the oocytes approached maximum size. Thereafter RNA polymerase activities declined rapidly and were very low in the oocytes of large, antral follicles. Administration of PMSG to mice had no significant effect on the polymerases of oocytes of small follicles or during the early stages of follicles growth when compared with saline-treated controls. However, in oocytes which had attained maximum size, a statistically significant increase in nucleolar labelling was detected after PMSG treatment. The elevated levels of RNA polymerase activity were confined to oocytes within this growth compartment. These observations are discussed in relation to the way the production of gene transcripts is regulated during the growth of the oocyte, both with respect to the storage of informational molecules and the development of meiotic maturation competence.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"56 1","pages":"1409-1417"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77906708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The morphological maturation of Bergmann glia cells, which might play a particularly important role in the histogenesis of the cerebellar cortex, was studied in GolgiCox preparations and electron micrographs of the cerebellum of normal and undernourished young rats. An unexpected acceleration in the morphological development of Bergmann glia cells was observed in the undernourished animals during the early postnatal period.
{"title":"Early effects of undernutrition on the development of cerebellar Bergmann glia cells","authors":"J. Clos, C. Legrand, J. Legrand","doi":"10.1051/RND:19790204","DOIUrl":"https://doi.org/10.1051/RND:19790204","url":null,"abstract":"The morphological maturation of Bergmann glia cells, which might play a particularly important role in the histogenesis of the cerebellar cortex, was studied in GolgiCox preparations and electron micrographs of the cerebellum of normal and undernourished young rats. An unexpected acceleration in the morphological development of Bergmann glia cells was observed in the undernourished animals during the early postnatal period.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"49 1","pages":"167-172"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76291677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Schilling, D. Smidt, B. Sacher, D. Petac, S. Kaschab
Summary. A fluorescence microscopy technique using diacetylfluorescin (FDA) as a substrate has been tested for the evaluation of the viability of early bovine embryos. Five to 8-day old cattle embryos were incubated in PBS containing FDA concentrations of 1 : 400 000 or 1 : 800 000 for 3 to 5 min at room temperature. Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Zeiss Axiomat microscope. Their mitotic activity after 24 hrs culture in vitro was used to determine their viability. After 3 min of incubation in the FDA medium, 85 p. 100 of the brilliantly fluorescing embryos showed mitoses after culture. None of the non-fluorescing embryos developed in vitro. Some embryos (about 6 p. 100) contained both dead and living cells as determined using the FDA test ; a few of these differentially reactive embryos were viable, but most of them were not. Short-term incubation of embryos in FDA medium probably did not impair their development, and no teratogenic effects could be seen in 17 to 20-day old rabbit fetuses transferred after the FDA test.
{"title":"Diagnosis of the viability of early bovine embryos by fluorescence microscopy","authors":"E. Schilling, D. Smidt, B. Sacher, D. Petac, S. Kaschab","doi":"10.1051/RND:19790924","DOIUrl":"https://doi.org/10.1051/RND:19790924","url":null,"abstract":"Summary. A fluorescence microscopy technique using diacetylfluorescin (FDA) as a substrate has been tested for the evaluation of the viability of early bovine embryos. Five to 8-day old cattle embryos were incubated in PBS containing FDA concentrations of 1 : 400 000 or 1 : 800 000 for 3 to 5 min at room temperature. Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Zeiss Axiomat microscope. Their mitotic activity after 24 hrs culture in vitro was used to determine their viability. After 3 min of incubation in the FDA medium, 85 p. 100 of the brilliantly fluorescing embryos showed mitoses after culture. None of the non-fluorescing embryos developed in vitro. Some embryos (about 6 p. 100) contained both dead and living cells as determined using the FDA test ; a few of these differentially reactive embryos were viable, but most of them were not. Short-term incubation of embryos in FDA medium probably did not impair their development, and no teratogenic effects could be seen in 17 to 20-day old rabbit fetuses transferred after the FDA test.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"25 1","pages":"1625-1629"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75882948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary. Influence of glucose and fructose on intestinal and hepatic metabolism in the rat in vivo. Intestinal and hepatic metabolism has been studied in rats fed 12-day diets comprising 74 p. 100 of either glucose or fructose. Food was available during 8 hrs, and tissue was sampled on anesthetized animals 6 hrs after the beginning of food intake. In the digestive tract, fructose was poorly converted into glucose (less than 10 p. 100), and enterocyte lactate production was similar with both hexoses, representing less than 10 p. 100 of the absorbed carbohydrates. About 50 p. 100 of the absorbed glucose was taken up by the liver, as against more than 90 p. 100 of the fructose. Considering the transformation of fructose into glucose, the hepatic carbohydrate balance was very similar with the two diets in our sampling condi- tions. Decreased liver uptake of lactate accounted for the increased lactataemia with the fructose diet. This fact was possibly due to an increase of hepatic triose phosphate and pyru- vate production, as fructose was mainly utilized in the fructose-1 phosphate pathway. Depletion of the hepatic ATP, induced by acute administration of fructose, was not observed in our adapted rats. As glycogen storage was more rapid with the fructose diet, triglyceride synthesis (an alternative pathway for the utilization of carbohydrates) certainly increased. This fact could account for the higher plasma triglycerides with the fructose diet, possibly associated with a lower response to the circulating insulin.
总结。葡萄糖和果糖对大鼠体内肠道和肝脏代谢的影响。研究人员对老鼠的肠道和肝脏代谢进行了研究，这些老鼠在12天的饮食中分别含有74 p. 100的葡萄糖或果糖。在8小时内提供食物，并在开始进食后6小时对麻醉动物进行组织取样。在消化道中，果糖很难转化为葡萄糖(少于10 p. 100)，肠细胞乳酸的产量与两种己糖相似，占吸收的碳水化合物的不到10 p. 100。被吸收的葡萄糖约有50%被肝脏吸收，而果糖则有90%以上被肝脏吸收。考虑到果糖转化为葡萄糖，在我们的采样条件下，肝脏碳水化合物平衡与两种饮食非常相似。肝脏乳酸摄取减少是果糖饮食导致乳酸血症增加的原因。这可能是由于果糖主要在果糖-1磷酸途径中被利用，肝脏中磷酸三糖和磷酸丙酮酸的产生增加。在我们的适应大鼠中，没有观察到由急性给药果糖引起的肝脏ATP的消耗。由于糖原储存在果糖饮食中更快，甘油三酯合成(利用碳水化合物的另一种途径)肯定会增加。这一事实可以解释果糖饮食中血浆甘油三酯较高的原因，这可能与对循环胰岛素的反应较低有关。
{"title":"Etude in vivo chez le rat de l'influence du glucose et du fructose sur le métabolisme intestinal et hépatique","authors":"E. Gueux, C. Demigné, C. Rémésy","doi":"10.1051/RND:19790603","DOIUrl":"https://doi.org/10.1051/RND:19790603","url":null,"abstract":"Summary. Influence of glucose and fructose on intestinal and hepatic metabolism in the rat in vivo. Intestinal and hepatic metabolism has been studied in rats fed 12-day diets comprising 74 p. 100 of either glucose or fructose. Food was available during 8 hrs, and tissue was sampled on anesthetized animals 6 hrs after the beginning of food intake. In the digestive tract, fructose was poorly converted into glucose (less than 10 p. 100), and enterocyte lactate production was similar with both hexoses, representing less than 10 p. 100 of the absorbed carbohydrates. About 50 p. 100 of the absorbed glucose was taken up by the liver, as against more than 90 p. 100 of the fructose. Considering the transformation of fructose into glucose, the hepatic carbohydrate balance was very similar with the two diets in our sampling condi- tions. Decreased liver uptake of lactate accounted for the increased lactataemia with the fructose diet. This fact was possibly due to an increase of hepatic triose phosphate and pyru- vate production, as fructose was mainly utilized in the fructose-1 phosphate pathway. Depletion of the hepatic ATP, induced by acute administration of fructose, was not observed in our adapted rats. As glycogen storage was more rapid with the fructose diet, triglyceride synthesis (an alternative pathway for the utilization of carbohydrates) certainly increased. This fact could account for the higher plasma triglycerides with the fructose diet, possibly associated with a lower response to the circulating insulin.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"31 1","pages":"715-721"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80617955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Activités de la musculature gastro-intestinale et mouvements des contenus digestifs J. P. Laplace, C. Roman
{"title":"Activités de la musculature gastro-intestinale et mouvements des contenus digestifs","authors":"J. Laplace, C. Roman","doi":"10.1051/RND:19790625","DOIUrl":"https://doi.org/10.1051/RND:19790625","url":null,"abstract":"HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Activités de la musculature gastro-intestinale et mouvements des contenus digestifs J. P. Laplace, C. Roman","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"47 1","pages":"849-879"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77722246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catherine Juste, T. Corring, Georgette Fredou, A. Roger, A. V. Hecke
{"title":"Effet d'une interruption et de restitutions partielles du recyclage entéro-hépatique sur le niveau de l'excrétion biliaire chez le porc","authors":"Catherine Juste, T. Corring, Georgette Fredou, A. Roger, A. V. Hecke","doi":"10.1051/RND:19790309","DOIUrl":"https://doi.org/10.1051/RND:19790309","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"24 1","pages":"405-412"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80145827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Decuypere, E. Nouwen, E. Kuhn, R. Geers, H. Michels
temperatures in a forced-draught laboratory incubator. A low incubation temperature resulted in a longer incubation period, whereas eggs incubated at a higher temperature hatched sooner. In all the temperature groups, serum T3 and T4 levels increased during the incubation period studied. Whatever the total duration of incubation within the experimental conditions, maximal serum T3 and Tg levels were always obtained the day of pipping. Embryos having perforated the air-space membrane the day before pipping showed elevated serum T3, but not T4, levels as compared to embryos without perforation. The presence of high serum T3 levels in chick embryos after perforation of the air-space membrane, and the sharp increase in the T3/, ratio before pipping were indicative of the important role of T 3 in the processes of pipping and hatching. After days 16 to 17, depending on the incubation temperature, a plateau for heat production (measured by indirect calorimetry) was reached while serum T3 and T4 levels were still increasing. Following the event of pipping, there was a rapid increase in heat production. A plateau might be due to the physical impossibility of each embryo to react upon an increase in T3 and T4 secretion by an increment in oxygen consumption, and would not exclude a relation between iodohormone levels and thermogenesis during development.
{"title":"lodohormones in the serum of chick embryos and post-hatching chickens as influenced by incubation temperature. Relationship with the hatching process and thermogenesis","authors":"E. Decuypere, E. Nouwen, E. Kuhn, R. Geers, H. Michels","doi":"10.1051/RND:19791008","DOIUrl":"https://doi.org/10.1051/RND:19791008","url":null,"abstract":"temperatures in a forced-draught laboratory incubator. A low incubation temperature resulted in a longer incubation period, whereas eggs incubated at a higher temperature hatched sooner. In all the temperature groups, serum T3 and T4 levels increased during the incubation period studied. Whatever the total duration of incubation within the experimental conditions, maximal serum T3 and Tg levels were always obtained the day of pipping. Embryos having perforated the air-space membrane the day before pipping showed elevated serum T3, but not T4, levels as compared to embryos without perforation. The presence of high serum T3 levels in chick embryos after perforation of the air-space membrane, and the sharp increase in the T3/, ratio before pipping were indicative of the important role of T 3 in the processes of pipping and hatching. After days 16 to 17, depending on the incubation temperature, a plateau for heat production (measured by indirect calorimetry) was reached while serum T3 and T4 levels were still increasing. Following the event of pipping, there was a rapid increase in heat production. A plateau might be due to the physical impossibility of each embryo to react upon an increase in T3 and T4 secretion by an increment in oxygen consumption, and would not exclude a relation between iodohormone levels and thermogenesis during development.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"24 1","pages":"1713-1723"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81588480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effets de la malnutrition protéinocalorique et de la réalimentation équilibrée sur les activités lipasique et phospholipasique A2 du pancréas et du suc pancréatique de rat","authors":"J. Prost, Mahmoud-Nazih Bouziane, J. Belleville","doi":"10.1051/RND:19790623","DOIUrl":"https://doi.org/10.1051/RND:19790623","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"40 1","pages":"837-841"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85274471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The relations between the morphological aspect of superovulated embryos at a given day of recovery and their viability in vitro were studied in order to compose a test for estimating embryo quality. The observation of 994 embryos produced by 100 donors showed that at each day of recovery, from D 6 to D 10, the embryos were distributed into at least four classes according to morphological aspect. At D 11 and D 12 more than 95 p.100 of them were divided into only two classes : abnormal and hatched blastocysts. When they were in the zona pellucida on the day of recovery, 60 p. 100 or more of the apparently normal embryos hatched after culture in vitro. 26 p. 100 or less hatched in vitro when development was apparently retarded, and none of those having an abnormal aspect, according to our criteria, hatched. When they were already hatched from the zona pellucida at recovery, 80 p. 100 of the embryos showed an increase in size after 24 hrs. of culture in vitro. The percentage of normal embryos decreased from D 6 to D 7, and then remained stable until at least D 10. The percentage of retarded embryos increased from D 6 to D 7 and then decreased. A few retarded embryos were visible at days 11 and 12. The percentage of degenerate embryos increased from D 7 to D 8 and remained stable afterwards. The proportion of donors with more than 50 p. 100 of degenerate embryos increased from D 6 to D 12. The relation with development in vivo after transplantation has been discussed.
{"title":"Variable development of superovulated bovine embryos between day 6 and day 12","authors":"J. Renard, Y. Heyman","doi":"10.1051/RND:19790920","DOIUrl":"https://doi.org/10.1051/RND:19790920","url":null,"abstract":"The relations between the morphological aspect of superovulated embryos at a given day of recovery and their viability in vitro were studied in order to compose a test for estimating embryo quality. The observation of 994 embryos produced by 100 donors showed that at each day of recovery, from D 6 to D 10, the embryos were distributed into at least four classes according to morphological aspect. At D 11 and D 12 more than 95 p.100 of them were divided into only two classes : abnormal and hatched blastocysts. When they were in the zona pellucida on the day of recovery, 60 p. 100 or more of the apparently normal embryos hatched after culture in vitro. 26 p. 100 or less hatched in vitro when development was apparently retarded, and none of those having an abnormal aspect, according to our criteria, hatched. When they were already hatched from the zona pellucida at recovery, 80 p. 100 of the embryos showed an increase in size after 24 hrs. of culture in vitro. The percentage of normal embryos decreased from D 6 to D 7, and then remained stable until at least D 10. The percentage of retarded embryos increased from D 6 to D 7 and then decreased. A few retarded embryos were visible at days 11 and 12. The percentage of degenerate embryos increased from D 7 to D 8 and remained stable afterwards. The proportion of donors with more than 50 p. 100 of degenerate embryos increased from D 6 to D 12. The relation with development in vivo after transplantation has been discussed.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"31 1","pages":"1589-1598"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89531419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanisms governing onset of ovarian cyclicity at puberty in the lamb","authors":"D. Foster, K. Ryan","doi":"10.1051/RND:19790821","DOIUrl":"https://doi.org/10.1051/RND:19790821","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"1 1","pages":"1369-1380"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89535939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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