œstradiol gonadotrophin releasing Summary. Three experiments were carried out to determine the effects of OB and GnRH on beef suckler cows at various stages post partum. In experiment 1, the first post partum oestrus occurred at 36 ! 17 (mean ± SD), 44 ! 25 and 43 ! 22 days post partum for animals injected with 400 fL9 OB or 100 fL9 GnRH at days 15 and 30 post partum and controls, respectively. The first post partum ovulation, based on elevated plasma progesterone levels, occurred at 37 ! 17 (mean ± SD), 31 ! 18 and 34 ! 10 days post partum for these three groups, respectively. Neither OB nor GnRH increased the numbers of animals which exhibited LH peaks at day 15 or 30 post partum or which showed a subsequent rise in plasma progesterone. There was a high proportion of silent ovulations early in the post partum period. In experiment 2, the time from parturition to the first subsequent oestrus was 93 j- 18 days for animals given 20 fL9 of an LHRH analogue (Hoe 766) between days 37 and 77 post partum, and 86 ! 28 days for controls. Significantly more (P < 0.01) treated cows than controls had elevated plasma progesterone levels following the time of treatment. In experiment 3, animals given two injections of 100 mcg progesterone and one injection 20 fL9 Hoe 766 at two day intervals between days 26 and 87 post partum had an interval between calving and first subsequent cestrus of 81 ! 22 days (mean -L SD) and this interval for controls was 83 ! 13 days. Five of seven treated animals exhibited cestrus 21-22 days after treatment suggesting occurrence of a silent ovulation after treatment.
{"title":"The effects of œstradiol benzoate (OB) and gonadotrophin releasing hormone (GnRH) on reproductive activity in beef cows at different intervals post partum","authors":"S. Mawhinney, J. Roche, J. Gosling","doi":"10.1051/RND:19790919","DOIUrl":"https://doi.org/10.1051/RND:19790919","url":null,"abstract":"œstradiol gonadotrophin releasing Summary. Three experiments were carried out to determine the effects of OB and GnRH on beef suckler cows at various stages post partum. In experiment 1, the first post partum oestrus occurred at 36 ! 17 (mean ± SD), 44 ! 25 and 43 ! 22 days post partum for animals injected with 400 fL9 OB or 100 fL9 GnRH at days 15 and 30 post partum and controls, respectively. The first post partum ovulation, based on elevated plasma progesterone levels, occurred at 37 ! 17 (mean ± SD), 31 ! 18 and 34 ! 10 days post partum for these three groups, respectively. Neither OB nor GnRH increased the numbers of animals which exhibited LH peaks at day 15 or 30 post partum or which showed a subsequent rise in plasma progesterone. There was a high proportion of silent ovulations early in the post partum period. In experiment 2, the time from parturition to the first subsequent oestrus was 93 j- 18 days for animals given 20 fL9 of an LHRH analogue (Hoe 766) between days 37 and 77 post partum, and 86 ! 28 days for controls. Significantly more (P < 0.01) treated cows than controls had elevated plasma progesterone levels following the time of treatment. In experiment 3, animals given two injections of 100 mcg progesterone and one injection 20 fL9 Hoe 766 at two day intervals between days 26 and 87 post partum had an interval between calving and first subsequent cestrus of 81 ! 22 days (mean -L SD) and this interval for controls was 83 ! 13 days. Five of seven treated animals exhibited cestrus 21-22 days after treatment suggesting occurrence of a silent ovulation after treatment.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"140 1","pages":"1575-1587"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82710841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prématurité de la mise bas chez la truie et signification du poids à la naissance du porcelet","authors":"A. Aumaître, B. Deglaire, J. Lebost","doi":"10.1051/RND:19790215","DOIUrl":"https://doi.org/10.1051/RND:19790215","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"20 1","pages":"267-275"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82836794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. L’organe pinéal du Brochet (Esox lucius L.). III. Voies intrapinéales de conduction des messages photosensoriels J. Falcon, J. P. Mocquard
它是一个多学科的开放获取档案，用于科学研究文件的存储和传播，无论它们是否出版。这些文件可能来自法国或国外的教学和研究机构，也可能来自公共或私人研究中心。HAL开放多学科档案旨在存放和传播来自法国或外国教育和研究机构、公共或私人实验室的已发表或未发表的研究级科学文件。梭子鱼(Esox lucius L.)的松果体。三。光感觉信息的神经内传导通路J. Falcon, J. P. Mocquard
{"title":"L'organe pinéal du Brochet (Esox lucius L.). III. Voies intrapinéales de conduction des messages photosensoriels","authors":"J. Falcon, J. Mocquard","doi":"10.1051/RND:19790707","DOIUrl":"https://doi.org/10.1051/RND:19790707","url":null,"abstract":"HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. L’organe pinéal du Brochet (Esox lucius L.). III. Voies intrapinéales de conduction des messages photosensoriels J. Falcon, J. P. Mocquard","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"245 1","pages":"1043-1061"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89053227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Three sensitive and specific radioimmunological assays (RIA) using iodinated tracers have been developed for the quantitative measurement of naturally occurring juvenile hormone (JH). The antigens were prepared by binding the JH’s to human serum albumin. lodinated tracers were prepared by binding histamine to the JH molecule, and then subsequently binding one molecule of 125 per molecule of histamine. The RIA’s were carried out in 0.02 M phosphate buffer containing 0.1 p.100 of BSA. The assay detection limit was 20 pg for all the three juvenile hormones. RIA sensitivity (at B/BO = 50 p. 100) of JH I, JH, and JH, was 0.75, 1 and 2.6 ng/ml, respectively. Antiserum to JH 1 cross-reacted 7.3 p. 100 with J H 2 and 0.8 p. 100 with JH! ; antiserum to J H 2 cross-reacted 26 p. 100 with JH, and 12 p. 100,with JH 3, and antiserum to JH, cross-reacted 0.3 p. 100 with JH, and 0.9 p. 100 with JH 2’ Various methods of preparing biological samples for the RIA of JH’s have been described.
利用碘化示踪剂，建立了三种灵敏、特异的放射免疫测定法(RIA)，用于定量测定天然存在的幼年激素(JH)。将JH 's与人血清白蛋白结合制备抗原。通过将组胺与JH分子结合，然后将组胺与JH分子每125个结合，制备出lodinated示踪剂。RIA在含有0.1 p.100 BSA的0.02 M磷酸盐缓冲液中进行。三种幼体激素的检测限均为20 pg。JH I、JH和JH的RIA敏感性(B/BO = 50 p. 100)分别为0.75、1和2.6 ng/ml。jh1抗血清与jh2交叉反应7.3 p. 100，与jh2交叉反应0.8 p. 100。;jh2抗血清与JH的交叉反应为26p100，与jh3的交叉反应为12p100; JH抗血清与JH的交叉反应为0.3 p100，与jh2的交叉反应为0.9 p100。
{"title":"A radioimmunological assay for naturally occurring insect juvenile hormones using iodinated tracers : Its use in the analysis of biological samples","authors":"J. Baehr, P. Pradelles, F. Dray","doi":"10.1051/RND:19791018","DOIUrl":"https://doi.org/10.1051/RND:19791018","url":null,"abstract":"Three sensitive and specific radioimmunological assays (RIA) using iodinated tracers have been developed for the quantitative measurement of naturally occurring juvenile hormone (JH). The antigens were prepared by binding the JH’s to human serum albumin. lodinated tracers were prepared by binding histamine to the JH molecule, and then subsequently binding one molecule of 125 per molecule of histamine. The RIA’s were carried out in 0.02 M phosphate buffer containing 0.1 p.100 of BSA. The assay detection limit was 20 pg for all the three juvenile hormones. RIA sensitivity (at B/BO = 50 p. 100) of JH I, JH, and JH, was 0.75, 1 and 2.6 ng/ml, respectively. Antiserum to JH 1 cross-reacted 7.3 p. 100 with J H 2 and 0.8 p. 100 with JH! ; antiserum to J H 2 cross-reacted 26 p. 100 with JH, and 12 p. 100,with JH 3, and antiserum to JH, cross-reacted 0.3 p. 100 with JH, and 0.9 p. 100 with JH 2’ Various methods of preparing biological samples for the RIA of JH’s have been described.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"19 1","pages":"1827-1836"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78929583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary. A. Anatomo-pathological research on rats ingesting different doses of peanut oil or of low-erucic acid rapeseed oil (Primor rapeseed oil). 6a. Structure and fine structure of the myocardium. Structural and ultrastructural observations were carried out on the myocardium of Wistar rats receiving increasing amounts (5, 10, 15 p. 100 in weight) of either peanut oil or Primor rapeseed oil in the diet. After 6 months of diet, myocardial changes, although depending on dietary oil content, were more extensive in the rats fed Primor rapeseed oil than in those fed the peanut oil. After 1 year of the 5 p. 100 diet containing either peanut oil or Primor rapeseed oil, myocardial modifications were of the same order and type. These changes were more extensive than those observed after 6 months of diet. Les animaux différentes
总结。A.大鼠摄入不同剂量花生油或低芥酸菜籽油(初级菜籽油)的解剖病理研究。6个。心肌的结构和精细结构。在Wistar大鼠日粮中分别添加5、10、15、100、100重量的花生油或初级菜籽油，对其心肌进行了结构和超微结构观察。饮食6个月后，心肌变化，虽然取决于饮食中的油脂含量，在大鼠喂食初级菜籽油比那些喂食花生油更广泛。饲喂含花生油或初级菜籽油的5 p. 100日粮1年后，心肌改变的顺序和类型相同。这些变化比节食6个月后观察到的更广泛。Les animaux diffsamrentes
{"title":"A. Recherches anatomopathologiques chez le rat ingérant différentes doses d'huile d'arachide ou d'huile de colza à faible teneur en acide érucique (Huile de colza Primor). 6a. Etude structurale et ultrastructurale du myocarde","authors":"N. Vodovar, F. Desnoyers, S. Delpal, G. Paillard","doi":"10.1051/rnd:19790408","DOIUrl":"https://doi.org/10.1051/rnd:19790408","url":null,"abstract":"Summary. A. Anatomo-pathological research on rats ingesting different doses of peanut oil or of low-erucic acid rapeseed oil (Primor rapeseed oil). 6a. Structure and fine structure of the myocardium. Structural and ultrastructural observations were carried out on the myocardium of Wistar rats receiving increasing amounts (5, 10, 15 p. 100 in weight) of either peanut oil or Primor rapeseed oil in the diet. After 6 months of diet, myocardial changes, although depending on dietary oil content, were more extensive in the rats fed Primor rapeseed oil than in those fed the peanut oil. After 1 year of the 5 p. 100 diet containing either peanut oil or Primor rapeseed oil, myocardial modifications were of the same order and type. These changes were more extensive than those observed after 6 months of diet. Les animaux différentes","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"354 1","pages":"509-522"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76487363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Fajer, J. Schneider, D. McCall, I. Ances, S. E. Polakis
With a quantitative approach it was possible to characterize the meiosis inducing activities of the ovary and the extra ovarian structures (rete ovarii). While the ovarian inducing capacity rises with age and remains high on days 14 and 15 post partum at the end of the prophase of the first meiotic division, the rete ovarii shows meiosis inducing activity up to day 5 post partum and an inhibitory activity on meiosis onset in the ovary in the 14 and 15 day old animals. The early activity of the rete ovarii seems to be more of a maturing nature on the germ cells in meiosis.
{"title":"The induction of meiosis by ovaries of newborn hamsters and its relation to the action of the extra ovarian structures in the mesovarium (rete ovarii)","authors":"A. Fajer, J. Schneider, D. McCall, I. Ances, S. E. Polakis","doi":"10.1051/RND:19790811","DOIUrl":"https://doi.org/10.1051/RND:19790811","url":null,"abstract":"With a quantitative approach it was possible to characterize the meiosis inducing activities of the ovary and the extra ovarian structures (rete ovarii). While the ovarian inducing capacity rises with age and remains high on days 14 and 15 post partum at the end of the prophase of the first meiotic division, the rete ovarii shows meiosis inducing activity up to day 5 post partum and an inhibitory activity on meiosis onset in the ovary in the 14 and 15 day old animals. The early activity of the rete ovarii seems to be more of a maturing nature on the germ cells in meiosis.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"570 1","pages":"1273-1278"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87252738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
rone (AFT) and maternal peripheral plasma testosterone were radioimmunologically assayed. (1) In 57-day male fetuses, UAPT level was 3 to 4 ng/mi ; it dropped to 0.5 to 1 ng/ml near 75 days and stayed at that level until term. This content in female fetuses remained at about 1 ng/mi throughout gestation (fig. 1). (2) During the second-half of gestation, UVPT content was generally lower than UAPT content, whatever the fetal sex (table 1). (3) AFT level was clearly lower than UAPT or UVPT level ; it stayed under the sensitivity limit of the assay method in most of the female fetuses. The AFT concentration curve in male fetuses was parallel to that of UAPT (fig. 2). The correlation coefficient (r = 0.71) (fig. 3), together with the relatively large quantities of AFT in 37 to 50-day male fetuses, suggested that the fetal testis could secrete testosterone at a very early stage when the Leydig cells were not yet recognizable. (4) There was no relation between maternal testosteronemia and the sex of the conceptus at any time during gestation (fig. 4).
{"title":"Testosterone levels in umbilical cord blood, maternal peripheral plasma and amniotic fluid of the crab-eating monkey (Macaca fascicularis)","authors":"D. Dang, N. Meusy‐Dessolle","doi":"10.1051/RND:19790815","DOIUrl":"https://doi.org/10.1051/RND:19790815","url":null,"abstract":"rone (AFT) and maternal peripheral plasma testosterone were radioimmunologically assayed. (1) In 57-day male fetuses, UAPT level was 3 to 4 ng/mi ; it dropped to 0.5 to 1 ng/ml near 75 days and stayed at that level until term. This content in female fetuses remained at about 1 ng/mi throughout gestation (fig. 1). (2) During the second-half of gestation, UVPT content was generally lower than UAPT content, whatever the fetal sex (table 1). (3) AFT level was clearly lower than UAPT or UVPT level ; it stayed under the sensitivity limit of the assay method in most of the female fetuses. The AFT concentration curve in male fetuses was parallel to that of UAPT (fig. 2). The correlation coefficient (r = 0.71) (fig. 3), together with the relatively large quantities of AFT in 37 to 50-day male fetuses, suggested that the fetal testis could secrete testosterone at a very early stage when the Leydig cells were not yet recognizable. (4) There was no relation between maternal testosteronemia and the sex of the conceptus at any time during gestation (fig. 4).","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"06 1","pages":"1307-1316"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85984396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanisms governing onset of ovarian cyclicity at puberty in the lamb","authors":"D. Foster, K. Ryan","doi":"10.1051/RND:19790821","DOIUrl":"https://doi.org/10.1051/RND:19790821","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"1 1","pages":"1369-1380"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89535939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Les cycles alimentaires des Vipères européennes dans des conditions semi-naturelles H. Saint-Girons
{"title":"Les cycles alimentaires des Vipères européennes dans des conditions semi-naturelles","authors":"H. Saint-Girons","doi":"10.1051/RND:19790111","DOIUrl":"https://doi.org/10.1051/RND:19790111","url":null,"abstract":"HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Les cycles alimentaires des Vipères européennes dans des conditions semi-naturelles H. Saint-Girons","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"35 1","pages":"125-134"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85626232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Schilling, D. Smidt, B. Sacher, D. Petac, S. Kaschab
Summary. A fluorescence microscopy technique using diacetylfluorescin (FDA) as a substrate has been tested for the evaluation of the viability of early bovine embryos. Five to 8-day old cattle embryos were incubated in PBS containing FDA concentrations of 1 : 400 000 or 1 : 800 000 for 3 to 5 min at room temperature. Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Zeiss Axiomat microscope. Their mitotic activity after 24 hrs culture in vitro was used to determine their viability. After 3 min of incubation in the FDA medium, 85 p. 100 of the brilliantly fluorescing embryos showed mitoses after culture. None of the non-fluorescing embryos developed in vitro. Some embryos (about 6 p. 100) contained both dead and living cells as determined using the FDA test ; a few of these differentially reactive embryos were viable, but most of them were not. Short-term incubation of embryos in FDA medium probably did not impair their development, and no teratogenic effects could be seen in 17 to 20-day old rabbit fetuses transferred after the FDA test.
{"title":"Diagnosis of the viability of early bovine embryos by fluorescence microscopy","authors":"E. Schilling, D. Smidt, B. Sacher, D. Petac, S. Kaschab","doi":"10.1051/RND:19790924","DOIUrl":"https://doi.org/10.1051/RND:19790924","url":null,"abstract":"Summary. A fluorescence microscopy technique using diacetylfluorescin (FDA) as a substrate has been tested for the evaluation of the viability of early bovine embryos. Five to 8-day old cattle embryos were incubated in PBS containing FDA concentrations of 1 : 400 000 or 1 : 800 000 for 3 to 5 min at room temperature. Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Zeiss Axiomat microscope. Their mitotic activity after 24 hrs culture in vitro was used to determine their viability. After 3 min of incubation in the FDA medium, 85 p. 100 of the brilliantly fluorescing embryos showed mitoses after culture. None of the non-fluorescing embryos developed in vitro. Some embryos (about 6 p. 100) contained both dead and living cells as determined using the FDA test ; a few of these differentially reactive embryos were viable, but most of them were not. Short-term incubation of embryos in FDA medium probably did not impair their development, and no teratogenic effects could be seen in 17 to 20-day old rabbit fetuses transferred after the FDA test.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"25 1","pages":"1625-1629"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75882948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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