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New attempts to decrease the variability of ovarian response to PMSG in cattle 减少牛卵巢对PMSG反应变异性的新尝试
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790911
D. Chupin, J. Saumande
The variability of ovarian response in cattle after superovulatory treatment with PMSG is mainly due to differences in the follicular population on the day of PMSG injection. We have tried to modify this follicular population either by destroying some of the follicles at the time of PMSG injection or by pretreatment at the beginning of the cycle. The electrocauterization of follicles on day 16 delayed luteolysis and did not reduce variability. The injection of 1 000 i.u. of PMSG on day 3 post-cestrus produced 35.7 p. 100 of twin ovulations when 600 i.u. were injected on day 16. Another explanation of response variability is the duration of PMSG action which can be modified by controlling the PMSG-oestrus interval with prostaglandin analogs; lenghtening that interval increased the ovulation rates from 1.75 to 4.60 and decreased the number of large follicles growing after ovulation from 5.13 to 0.60. Moreover, when PMSG prostaglandin treatment was associated with progestagen, PMSG efficiency increased. So after 500 i.u. of PMSG at the end of a progestagenprostaglandin treatment we obtained à 34.1 p. 100 twinning rate. Finally, repeated treatments (2 000 i.u. PMSG-0.5 mg PGF 2a analog) every 6 weeks during one year produced the same variability in the response pattern : response was constant in 29.8 p. 100 of the heifers, 27.7 p. 100 showed a definite decrease after 3 to 5 treatments, and 36.2 p. 100 a decrease followed by an increase.
用PMSG治疗牛超排卵后卵巢反应的差异主要是由于注射PMSG当天卵泡数量的差异。我们试图通过在注射PMSG时破坏一些卵泡或在周期开始时进行预处理来改变卵泡数量。电灼第16天的卵泡延迟了黄体溶解,并没有降低变异性。孕后第3天注射1 000 iu的PMSG,第16天注射600 iu的PMSG,双胎排卵率为35.7%。反应变异性的另一种解释是PMSG作用的持续时间,可以通过前列腺素类似物控制PMSG-发情间隔来改变;延长该间隔可使排卵率从1.75提高到4.60,使排卵后生长的大卵泡数从5.13减少到0.60。此外,当前列腺素治疗与孕激素治疗相结合时,PMSG的效率增加。因此,在孕前列腺素治疗结束时使用500 iu的PMSG后,我们获得了 34.1 p. 100的双胞胎率。最后,在一年中,每6周重复处理一次(2 000 iu PMSG-0.5 mg PGF 2a类似物),在反应模式上产生了相同的变化:29.8 p. 100的小牛的反应是恒定的,27.7 p. 100在3到5次处理后明显下降,36.2 p. 100先下降后增加。
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引用次数: 19
Measurement of the rate of cholesterol synthesis in various organs of the rat in vivo 测定大鼠体内各器官的胆固醇合成速率
Pub Date : 1979-01-01 DOI: 10.1051/RND:19791012
T. Magot, F. Chevallier
A method to measure the absolute values of the rate of cholesterol synthesis in vivo in the organs of the rat has been described. Adult male rats were intravenously infused for 3.5, 6 and 10 h with sodium 1-4 (C)-acetate and the specific activity of expired C0 2 was recorded during the experiment. Sterol radioactivities were measured in plasma, lipoproteins and in various organs, the radioactivities in each organ being corrected for cholesterol transfer. The rate of cholesterol synthesis in the intact rat and in each organ
描述了一种测量体内大鼠器官中胆固醇合成速率绝对值的方法。成年雄性大鼠分别静脉注射1-4 (C)-乙酸钠3.5、6和10 h,并在实验过程中记录过期co2的比活性。在血浆、脂蛋白和各种器官中测量甾醇放射性,每个器官中的放射性被校正为胆固醇转移。完整大鼠及各器官胆固醇合成率
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引用次数: 11
Some relationships among germ, satellite and interstitial cells during chick gonad differentiation : A tissue culture study 鸡性腺分化过程中胚细胞、卫星细胞和间质细胞关系的组织培养研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790812
J. Jordanov, P. Angelova
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引用次数: 1
Passage d'éléments d'origine microbienne à travers la muqueuse intestinale du lapin. Étude autoradiographique après marquage des microorganismes par la glycine tritiée 微生物来源的元素通过兔子肠道粘膜的通道。氚化甘氨酸标记微生物后的自射线照相研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790506
M. Petitprez, R. Moncoulon, L. Albertini, P. Raynaud
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引用次数: 0
The effects of Isopropyl-N-Phenylcarbomate on meiotic maturation of mammalian oocytes 异丙基苯碳酸酯对哺乳动物卵母细胞减数分裂成熟的影响
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790713
N. Crozet, D. Szöllösi
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. The effects of Isopropyl-N-Phenylcarbomate on meiotic maturation of mammalian oocytes Nicole Crozet, D. Szöllösi
它是一个多学科的开放获取档案,用于科学研究文件的存储和传播,无论它们是否出版。这些文件可能来自法国或国外的教学和研究机构,也可能来自公共或私人研究中心。HAL开放多学科档案旨在存放和传播来自法国或外国教育和研究机构、公共或私人实验室的已发表或未发表的研究级科学文件。异丙基- n -苯基碳酸酯对哺乳动物卵母细胞减数成熟的影响
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引用次数: 7
Absorption kinetics of amino acids and reducing sugars during digestion of barley or wheat meals in the pig : Preliminary data 猪在消化大麦或小麦时氨基酸和还原糖的吸收动力学:初步数据
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790607
A. Rérat, P. Vaissade, P. Vaugelade
Summary. The digestion of barley and wheat was studied in the pig using a metho- dology quantitatively describing the apparent absorption kinetics of amino acids and reducing sugars. This methodology is based on the simultaneous and continuous measure- ment of the postprandial increase of the level of these nutrients in the efferent blood of the digestive tract (porto-arterial differences) and the measurement of the portal vein blood flow. After an 18-hr fast, the experimental meals (18 for barley, 22 for wheat) were given to animals weighing 40 to 50 kg in amounts varying from 200 to 1 600 g ; recordings were taken over a periprandial period of 10 to 12 hrs. The following main results were obtained : essential amino acids, aromatic amino acids and methionine appeared more rapidly. On the other hand, arginine and cystine appeared more they were metabolized in the digestive wall. Among the non-essential amino acids, glutamine and glutamic acid appeared very slowly and alanine and glycocolle very quickly and intensely ; non-alimentary products such as citrulline and ornithine were synthesized. Absorption balance after 8 hrs of digestion, as shown by absorption percentages, ranged from 71 p.100 (histidine) to 20 p. 100 (cystine) for barley and between 90 and 29 p. (same amino acids) case of both and glycine in the case of were synthe- sized. The absorption coefficient of glutamic and glutamine very cases.
总结。用定量描述氨基酸和还原糖表观吸收动力学的方法研究了大麦和小麦在猪体内的消化。这种方法是基于同时和连续测量餐后消化道出血中这些营养物质水平的增加(门动脉差异)和门静脉血流的测量。禁食18小时后,给体重40 ~ 50公斤的动物喂食实验饲粮(大麦18餐,小麦22餐),量从200 ~ 1600克不等;在餐后10至12小时内进行记录。主要结果如下:必需氨基酸、芳香氨基酸和蛋氨酸的出现速度较快。另一方面,精氨酸和胱氨酸出现更多,它们在消化壁代谢。在非必需氨基酸中,谷氨酰胺和谷氨酸的出现非常缓慢,丙氨酸和糖基的出现非常迅速和强烈;合成了瓜氨酸和鸟氨酸等非消化产物。消化8小时后的吸收平衡,如吸收百分比所示,范围从71 p.100(组氨酸)到20p . 100(胱氨酸)大麦和90和29p .(相同的氨基酸)之间的情况下,甘氨酸的情况下,合成。谷氨酸和谷氨酰胺的吸收系数非常高。
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引用次数: 24
Mesure du débit du sang de la veine porte au moyen d'un débitmètre électromagnétique chez la chèvre 用电磁流量计测量山羊门静脉的血流量
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790606
J. Barry, Elisabeth Debras, J. Lefaivre, C. Champredon
Summary. Measurement of portal vein blood flow in goats using an electromagnetic flow- meter. When quantitatively measuring the products of digestion in the portal blood, the following factors are to be studied : - the difference between the concentrations of those products in the arterial blood affe- rent to the alimentary canal and of those in the venous portal blood, - blood flow in the portal vein. As a preliminary step, we have undertaken the study of the continuous measurement of the portal blood flow in ruminants (goats), using a method analogous to that employed by Rérat in pigs. Nine goats were fitted with a ring-probe around the portal vein ; during reading, the probe was attached to an electromagnetic flow-meter connected to a recor-der. Fifteen readings were taken on 4 of the animals (mean live weight : 47 kg) for about 7 hours after the morning feeding. After installation, each gauge allowed readings for 4 to 5 consecutive weeks. The signal then faded, and the animals were slaughtered and exami- ned. As the wall of the portal vein had slightly thickened and therefore its lumen was only slightly reduced, signal damping could be attributed to a change in probe functio- ning. The mean flow, measured at the time of reading, was 1 830 ml/min or 39 ml/min/kg of live weight. This study showed a large variability in the flow and distinguished 3 variations throughout the day :
总结。用电磁流量计测量山羊门静脉血流。当定量测量门静脉血液中的消化产物时,需要研究以下因素:流向消化道的动脉血中消化产物的浓度与门静脉血液中消化产物的浓度之间的差异;门静脉血流。作为初步步骤,我们已经开展了反刍动物(山羊)门静脉血流连续测量的研究,使用的方法类似于rsamrat在猪身上使用的方法。9只山羊在门静脉周围安装环状探针;读数时,探头连接到与记录仪相连的电磁流量计上。在早上喂食后约7小时内,对其中4只动物(平均活重:47 kg)进行了15次读数。安装后,每个仪表允许连续读数4至5周。然后信号消失了,动物被宰杀并接受检查。由于门静脉壁略有增厚,因此其管腔仅略有减少,信号衰减可归因于探头功能的改变。读数时测量的平均流量为1 830 ml/min或39 ml/min/kg活重。这项研究显示了流量的巨大变化,并在一天中区分了3种变化:
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引用次数: 3
Follicular populations in the ewe under different gonadotrophin levels 不同促性腺激素水平下母羊卵泡群的变化
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790909
L. Cahill, J. Dufour
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Follicular populations in the ewe under different gonadotrophin levels L. P. Cahill, J. Dufour
它是一个多学科的开放获取档案,用于科学研究文件的存储和传播,无论它们是否出版。这些文件可能来自法国或国外的教学和研究机构,也可能来自公共或私人研究中心。HAL开放多学科档案旨在存放和传播来自法国或外国教育和研究机构、公共或私人实验室的已发表或未发表的研究级科学文件。不同促性腺激素水平下ewe的卵泡种群L. P. Cahill, J. Dufour
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引用次数: 6
Plasma steroids, prolactin and placental lactogen in intact or hypophysectomized pregnant ewes with an hypophysectomized foetus. Effect of foetal perfusion of dexamethasone 完整或去垂体妊娠母羊与去垂体胎儿的血浆类固醇、催乳素和胎盘乳素。地塞米松对胎儿灌注的影响
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790710
M. Bosc, P. Durand, A. Locatelli, A. Nicolle, J. Ravault, M. Terqui
Progesterone, total oestrogens (mainly conjugated and unconjugated oestrone), prolactin and placental lactogen have been determined during late pregnancy in the peripheral plasma of intact or hypophysectomized ewes after destruction of the foetal pituitary. During the prolongation of gestation due to the removal of the foetal hypophysis, progesterone and placental lactogen remained at a high level, while total oestrogens and prolactin maintained a low concentration. When the foetus was perfused for 2 days with dexamethasone, the induced lambing was preceded by a fall in progesterone concentrations followed by a decrease in placental lactogen ; at the same time, an abrupt increase in oestrogens and prolactin was observed.
胎儿垂体破坏后,在妊娠后期检测了完整或去垂体母羊外周血血浆中孕酮、总雌激素(主要是偶联和未偶联的雌激素)、催乳素和胎盘乳素的含量。在去除胎儿垂体延长妊娠期间,孕酮和胎盘乳素保持较高水平,总雌激素和催乳素维持较低浓度。地塞米松灌注2 d后,诱导羔的孕酮浓度下降,胎盘乳原减少;同时,观察到雌激素和催乳素的突然增加。
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引用次数: 4
Selective protein incorporation by vitellogenic Salmo gairdneri oocytes in vitro 体外产卵黄小龙虾卵母细胞选择性蛋白掺入研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790311
C. M. Campbell, B. Jalabert, G. Campbell, M. Heydorff
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引用次数: 38
期刊
Annales De Biologie Animale Biochimie Biophysique
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