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Increase of follicle cell LH binding and changes in the LH level of follicular fluid during the preovulatory period in the sow 母猪排卵期卵泡细胞LH结合增加及卵泡液LH水平变化
Pub Date : 1979-01-01 DOI: 10.1051/RND:19791003
M. Daguet
This study used the technique of histoautoradiography to analyze the variation of LH binding in sow follicles during the preovulatory period. Estrus was synchronized by methallibure to obtain a reference point for dating the onset of the follicular phase of each sow in the experiment. In follicles increasing in diameter from 2.6 mm at days 0 and 1 of the follicular phase to 6.6 mm at day 6, granulosa 125 _oLH or 125 -pLH binding increased by 14 times while that of the theca interna increased by 2. The binding of these hormones remained high during and after the end of the ovulatory LH surge, at least until several hours before ovulation. Variations in plasmic as well as intrafollicular LH levels were radioimmunologically assayed. High intrafollicular levels of estradiol-1 7p at day 3 would explain the large increase of granulosa LH binding between days 4 and 5-6, in turn causing the intrafollicular level of progesterone to rise at day 5. These results indicated an interdependence between hormonal levels and target cell receptivity.
本研究采用组织放射自显影技术对母猪卵泡LH结合变化进行了分析。通过甲基化测定来同步发情,以获得测定试验中每头母猪卵泡期开始的参考点。卵泡从卵泡期第0和第1天的2.6 mm增加到第6天的6.6 mm时,125 _oLH或125 -pLH颗粒结合增加了14倍,而卵泡内颗粒结合增加了2倍。在促黄体生成素激增期间和结束后,这些激素的结合仍然很高,至少直到排卵前几个小时。用放射免疫学方法检测血浆和卵泡内LH水平的变化。第3天的高卵泡内雌二醇-1 7p水平可以解释第4天和第5-6天之间颗粒LH结合的大量增加,从而导致第5天黄体酮的卵泡内水平升高。这些结果表明激素水平和靶细胞接受性之间存在相互依赖关系。
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引用次数: 19
Induction of intestinal sucrase-isomaltase in suckling rats after hydrocortisone injections and substrate administration 氢化可的松注射和底物给药对哺乳大鼠肠道蔗糖-异麦芽糖酶的诱导作用
Pub Date : 1979-01-01 DOI: 10.1051/rnd:19790616
F. Raul, P. Simon, M. Kedinger, K. Haffen
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引用次数: 0
Influence de l'heure d'un repas unique sur la rétention de Ca, K et P et sur l'index de coquille chez la poule pondeuse 单餐时间对蛋鸡钙、钾、磷滞留及蛋壳指数的影响
Pub Date : 1979-01-01 DOI: 10.1051/rnd:19790642
Y. Nys, H. Antoine, J. Rocard
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引用次数: 0
Fetal and postnatal metabolism in the calf 小牛的胎儿和产后代谢
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790203
C. Demigné, C. Rémésy
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引用次数: 9
Influence des facteurs sexuels, chez le rat, sur l'activité des phosphatases alcalines intestinales. Etude immunologique 性别因素对大鼠肠道碱性磷酸酶活性的影响。免疫学研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790617
Nicole Fontaine
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Influence des facteurs sexuels, chez le rat, sur l’activité des phosphatases alcalines intestinales. Etude immunologique Nicole Fontaine
它是一个多学科的开放获取档案,用于科学研究文件的存储和传播,无论它们是否出版。这些文件可能来自法国或国外的教学和研究机构,也可能来自公共或私人研究中心。HAL开放多学科档案旨在存放和传播来自法国或外国教育和研究机构、公共或私人实验室的已发表或未发表的研究级科学文件。性别因素对大鼠肠道碱性磷酸酶活性的影响。免疫学研究Nicole Fontaine
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引用次数: 0
Excrétion biliaire chez le porc : niveau et réponse au repas 猪胆道排泄:水平及对膳食的反应
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790106
Catherine Juste, T. Corring, Ph. Breant, Georgette Fredou, A. Roger, A. V. Hecke
A technique for permanent fistulation of the bile duct, associated with a permanent fistulation of the duodenum allowing continuous reintroduction of the collected bile, was used to investigate the level and daily kinetics of biliary excretion in the conscious pig. Seven Large-White pigs of 45 kg live weight were fistulated. Volumes and total excreted bile salts were measured in hourly samples. Results show that within 24 hrs.the volume of the collected bile was about 2100 ml and the total amount of bile salts about 37 g. The bile excretion (volume and bile salts) was minimum before the morning meal (9:00 a. m.) and the total amount of the excreted bile salts immediately increased after diet intake. Within 2 hrs. after the morning and afternoon meals, values were about 300 p. 100 higher than those determined at 9:00 a. m. This increase was essentially the result of the excretion of a highly concentrated bile (200 to 300 p. 100 more than the values at 9:00 a. m.). On the other hand, the bile volumes were only affected 2 to 3 hrs. after diet intake.
一种永久性的胆管瘘管技术,与十二指肠的永久性瘘管相结合,允许收集的胆汁不断重新引入,用于研究有意识的猪的胆管排泄水平和每日动力学。对7头45 kg活重的大白猪进行瘘管。每小时取样测量胆汁盐的体积和总排泄量。结果表明,在24小时内。收集胆汁体积约2100 ml,胆汁盐总量约37 g。在早餐前(上午9:00)胆汁排泄量(体积和胆盐)最少,饮食后胆汁盐总量立即增加。2小时内。在上午和下午用餐后,该数值比上午9:00时测定的数值高出约300p . 100。这种增加主要是由于高浓度胆汁的排泄(比上午9:00时的数值高出200至300p . 100)。另一方面,胆汁容量仅在2至3小时内受到影响。饮食后摄入。
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引用次数: 19
Importance de la voie vagale sensitive : recherche d'une technique de déafférentation sélective chez le porc et le mouton 感觉迷走神经通路的重要性:猪和羊选择性分离技术的研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790626
B. Darcy, M. Falempin, J. Laplace, J. Rousseau, C. Germain, A. Rousseau, P. Villiers
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引用次数: 10
Improvement in gonadal function through modification of negative feed-back 通过修改负反馈改善性腺功能
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790918
R. B. Land
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引用次数: 6
Effect of stress on the course of labor and parturition time in normal or adrenalectomized rats 应激对正常或去肾上腺大鼠产程及分娩时间的影响
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790102
M. Bosc, A. Nicolle
were applied after expulsion of the first fetus. A simple manipulation of the female or a change of territory did not modify the whole expulsion phase but increased variability in the birth of the second fetus. By comparison, a strong stress lengthened the duration of expulsion and influenced the time intervals between the first births. A comparison of these results with those obtained after adrenalectomy indicated that the inhibitory effect of a strong stress was probably due to maternal adrenal epinephrin secretion. In the second experiment, a stress was applied before labor in normal rats submitted to two light regimes. In these conditions, the treatment essentially stimulated the last parturitions, which occurred earlier than in control animals.
在排出第一个胎儿后使用。对雌性的简单操纵或领土的改变并不能改变整个驱逐阶段,但增加了第二个胎儿出生的可变性。相比之下,强烈的压力延长了驱逐的时间,并影响了第一次生育之间的时间间隔。将这些结果与肾上腺切除术后得到的结果进行比较,表明强应激的抑制作用可能是由于母体肾上腺素的分泌。在第二个实验中,将正常大鼠置于两种光照条件下,在分娩前施加压力。在这些情况下,治疗基本上刺激了最后一次分娩,比对照动物发生得更早。
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引用次数: 8
Effet de la sécrétion biliaire sur la digestion chez le porc 胆道分泌物对猪消化的影响
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790712
T. Corring, Catherine Juste, C. Simoes-Nunes, D. Bourdon, A. Gueugneau, J. P. Hauducœur, A. Roger, R. Levrel, G. Duchastel
The apparent digestibility of a diet was studied either after ligature of the bile duct or after discontinuous removal of bile secretion in the growing pig. Feces and urine were collected during a 10-day period in bile duct-ligated pigs, and over three 5-day periods in bile-fistulated pigs. The latter were subjected to three treatments each over the three periods : 1) re-entry of bile into the duodenum ; 2) introduction of a salt mixture (Na : 150 meq/1 ; CI : 120 meq/1 ; HC0 3: 40 meq/1) 3) no re-entry of either bile or the salt mixture. Apparent energy digestibility was mainly affected after ligature of the bile duct (-31.6 p. 100) or after removal of bile from the intestinal lumen (-23 p. 100). This decrease was essentially due to a decrease in the total lipid apparent digestibility (― 53 p. 100 after bile duct ligation and ― 31.7 p. 100 after bile removal). Apparent nitrogen and energy derived from carbohydrates digestibilities were unaffected. In the fistulated pigs the intraduodenal introduction of the salt mixture did not improve decreased apparent digestibilities.
研究了生长猪在结扎胆管或间断去除胆汁分泌后对饲粮的表观消化率。胆管结扎猪在10天内收集粪便和尿液,胆管瘘管猪在3个5天内收集粪便和尿液。后者分别在三个时期进行三种治疗:1)胆汁重新进入十二指肠;2)引入盐混合物(Na: 150 meq/1;CI: 120 meq/1;hc30:40 meq/1) 3)没有胆汁或盐混合物重新进入。表观能量消化率主要在结扎胆管(-31.6 p. 100)或从肠腔取出胆汁(-23 p. 100)后受到影响。这种减少主要是由于总脂质表观消化率(―53 p. 100胆管结扎术后;31.7 p. 100取胆后)。表观氮和来自碳水化合物消化率的能量不受影响。在有瘘管的猪中,十二指肠内引入盐混合物并没有改善下降的表观消化率。
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引用次数: 5
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Annales De Biologie Animale Biochimie Biophysique
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