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Regulation of meiosis in mammals 哺乳动物减数分裂的调控
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790809
A. Byskov
Culture media which contain either substances that can induce meiosis (meiosis-inducing substances, MIS) or prevent meiosis (meiosis-preventing substances, MPS) can be obtained from different experiments. MIS is present in culture media in which tissues of ovaries with germ cells in meiosis, or fetal epididymis, or puberal epididymis and testes, have grown. MIS is apparently species-nonspecific since media containing MIS obtained from puberal bulls induce meiosis in indifferent fetal mouse testes. MPS is secreted into the culture media of minced fetal mouse testes. Meiosis si prevented or greatly inhibited in fetal mouse ovaries, when they are cultured in such media. It was concluded that MIS was present in mesonephric derived tissues in gonads in which meiosis occurred. Moreover, MPS within the testicular tissue (within the testicular cords ?) might participate in preventing meiosis in the fetal testis. However, a negative « MIS-activity-test » result was obtained in an experiment with rabbit gonads : meiosis was not induced in fetal gonads by infant ovarian tissue containing germ cells in meiosis. This led to comparative investigations of gonads from species in which the onset of meiosis and gonadal sex differentiation coincide in time, « simultaneous meiosis », and those, like the rabbit, in which the onset of meiosis in the female is delayed,
可以从不同的实验中获得含有诱导减数分裂物质(减数分裂诱导物质,MIS)或防止减数分裂物质(减数分裂预防物质,MPS)的培养基。MIS存在于有减数分裂生殖细胞的卵巢组织、胎儿附睾或青春期附睾和睾丸生长的培养基中。从青春期公牛获得的含有MIS的培养基在不同的胎鼠睾丸中诱导减数分裂,因此MIS显然是非种特异性的。MPS分泌到切碎的胎鼠睾丸培养基中。当胚胎小鼠卵巢在这种培养基中培养时,减数分裂被阻止或大大抑制。结论:在发生减数分裂的性腺中肾源性组织中存在MIS。此外,睾丸组织内(睾丸索内)的MPS可能参与防止胎儿睾丸减数分裂。然而,在兔性腺实验中获得了阴性的“misi活性试验”结果:在减数分裂中含有生殖细胞的婴儿卵巢组织不能诱导胎儿性腺减数分裂。这导致了对减数分裂和性腺性别分化在时间上重合的物种的性腺的比较研究,“同时减数分裂”,以及像兔子这样的雌性减数分裂延迟的物种,
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引用次数: 74
L'organe pinéal du Brochet (Esox lucius, L.). I. Etude anatomique et cytologique 梭子鱼(Esox lucius, L.)的松果体。一、解剖和细胞学研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790313
J. Falcon
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引用次数: 25
Testosterone levels in umbilical cord blood, maternal peripheral plasma and amniotic fluid of the crab-eating monkey (Macaca fascicularis) 食蟹猴脐带血、母体外周血浆和羊水中睾酮水平的研究
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790815
D. Dang, N. Meusy‐Dessolle
rone (AFT) and maternal peripheral plasma testosterone were radioimmunologically assayed. (1) In 57-day male fetuses, UAPT level was 3 to 4 ng/mi ; it dropped to 0.5 to 1 ng/ml near 75 days and stayed at that level until term. This content in female fetuses remained at about 1 ng/mi throughout gestation (fig. 1). (2) During the second-half of gestation, UVPT content was generally lower than UAPT content, whatever the fetal sex (table 1). (3) AFT level was clearly lower than UAPT or UVPT level ; it stayed under the sensitivity limit of the assay method in most of the female fetuses. The AFT concentration curve in male fetuses was parallel to that of UAPT (fig. 2). The correlation coefficient (r = 0.71) (fig. 3), together with the relatively large quantities of AFT in 37 to 50-day male fetuses, suggested that the fetal testis could secrete testosterone at a very early stage when the Leydig cells were not yet recognizable. (4) There was no relation between maternal testosteronemia and the sex of the conceptus at any time during gestation (fig. 4).
用放射免疫法测定rone (AFT)和母体外周血血浆睾酮水平。(1) 57日龄男胎UAPT水平为3 ~ 4 ng/mi;75天左右降至0.5 ~ 1 ng/ml,并一直维持到足月。在整个妊娠期间,女性胎儿的UVPT含量保持在1 ng/mi左右(图1)。(2)在妊娠后半段,无论胎儿性别如何,UVPT含量普遍低于UAPT含量(表1)。(3)AFT水平明显低于UAPT或UVPT水平;在大多数女性胎儿中,它保持在检测方法的灵敏度极限以下。男性胎儿的AFT浓度曲线与UAPT平行(图2),相关系数(r = 0.71)(图3),加之37 ~ 50天男性胎儿中AFT含量较多,提示胎儿睾丸在间质细胞尚未识别的非常早期阶段就能分泌睾酮。(4)在妊娠期间的任何时候,母体睾酮水平与胎儿性别没有关系(图4)。
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引用次数: 6
Ontogeny of responsiveness to gonadotrophins and prostaglandin E in the neonatal rat ovary 新生大鼠卵巢对促性腺激素和前列腺素E反应的个体发生
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790819
S. Lamprecht, B. Funkenstein, A. Nimrod
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引用次数: 3
Sertoli cell ultrastructure. II. Morphological effects of hypophysectomy in pubescent pigs 支持细胞超微结构。2垂体切除术对短毛猪的形态学影响
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790504
M. Chevalier
Summary. As in normal animals, the Sertoli cell population in pubescent pigs was composed of two morphological cell types : numerous cells with a light nucleus (type A) and some small cells with a dark nucleus (type B). This report studies the transformations occurring in this population during the 4 months following hypophysectomy. The various cellular components had different rhythms of modification ; no general, continuous involution of the components was observed. The nucleolar complex appearing in the nucleus was composed of three elements and persisted during the whole period studied. The majo-rity of A-cell cytoplasmic components disappeared during the post-operative period, except for some lysosomes which developed and the Golgi apparatus which persisted. The cellular junctions between Sertoli cells and those facing the remaining germ cells were unmodified. Three or 4 months after hypophysectomy, the whole of the membranous and ribosomal systems was reorganized and developed. Microtubules and microfilaments gradually disappeared. In some cases the specialized inter-Sertoli cell junctions lost their microfilamentary component. The B-cells had an extremely condensed aspect 1 month after hypophysectomy. Three or 4 months after the operation, cytoplasmic ramifications and the presence of numerous ribosomes apparently indicated a change in B-cell metabolism. The microfilaments disappeared gradually. In both cell types, the large lipid droplet persisted ; it was always found against the nucleus and often in a basal position.
总结。与正常动物一样,短毛猪的支持细胞群由两种形态细胞类型组成:大量具有浅色细胞核的细胞(a型)和一些具有深色细胞核的小细胞(B型)。本报告研究了在垂体切除术后4个月内该群体发生的转化。不同的细胞成分具有不同的修饰节奏;没有观察到组分的普遍连续对合。出现在细胞核中的核仁复合体由三种元素组成,并在整个研究期间持续存在。术后除部分溶酶体发育和高尔基体保留外,大部分a细胞细胞质成分消失。支持细胞与面对剩余生殖细胞的细胞连接未被修饰。垂体切除术后3 ~ 4个月,整个膜和核糖体系统重组和发育。微管和微丝逐渐消失。在某些情况下,特化的支持细胞间连接失去了微丝成分。垂体切除术后1个月,b细胞呈极浓缩状。术后3 - 4个月,细胞质分支和大量核糖体的出现明显表明b细胞代谢发生了变化。微丝逐渐消失。在两种细胞类型中,大脂滴持续存在;它总是贴着细胞核,而且经常在基底位置。
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引用次数: 7
Colostrum de vache : composition minrale et activit de la phosphatase alcaline 乳牛初乳:组成、微量成分和碱性磷酸酶活性
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790302
G. Linden, B. Maraval
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Colostrum de vache : composition minérale et activité de la phosphatase alcaline G. Linden, B. Maraval
它是一个多学科的开放获取档案,用于科学研究文件的存储和传播,无论它们是否出版。这些文件可能来自法国或国外的教学和研究机构,也可能来自公共或私人研究中心。HAL开放多学科档案旨在存放和传播来自法国或外国教育和研究机构、公共或私人实验室的已发表或未发表的研究级科学文件。牛初乳:碱性磷酸酶的矿物组成和活性
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引用次数: 5
Influence d'une élévation de température sur l'évolution de l'aminoacidémie et de l'ammoniémie après le repas chez la truite arc en ciel (Salmo gairdneri R.) 温度升高对虹鳟鱼餐后氨基酸中毒和氨血症发展的影响(Salmo gairdneri R.)
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790708
B. Fauconneau, P. Luquet
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引用次数: 12
Les cycles alimentaires des Vipères européennes dans des conditions semi-naturelles 欧洲蝰蛇在半自然条件下的进食周期
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790111
H. Saint-Girons
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Les cycles alimentaires des Vipères européennes dans des conditions semi-naturelles H. Saint-Girons
它是一个多学科的开放获取档案,用于科学研究文件的存储和传播,无论它们是否出版。这些文件可能来自法国或国外的教学和研究机构,也可能来自公共或私人研究中心。HAL开放多学科档案旨在存放和传播来自法国或外国教育和研究机构、公共或私人实验室的已发表或未发表的研究级科学文件。欧洲蝰蛇在半自然条件下的进食周期
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引用次数: 23
Dosage des strognes libres et conjugus dans le plasma priphrique de vache cyclique 循环牛卵黄血浆中游离雌激素和共轭雌激素的测定
Pub Date : 1979-01-01 DOI: 10.1051/RND:19791006
A. López-Sebastián, J. De
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引用次数: 7
Influence du niveau des apports énergétiques pendant la fin de la gestation sur l'insulinémie et l'aminoacidémie des brebis et de leurs agneaux 妊娠末期能量摄入水平对母羊和羔羊胰岛素血症和氨基酸血症的影响
Pub Date : 1979-01-01 DOI: 10.1051/RND:19790207
J. Grizard, P. Patureau-Mirand, M. Tissier, O. Bernard, G. Bayle, A. Brelurut, J. Leroux
This 2 x 2 factorial experiment (table 1) was carried out in the autumn on 22 Limousine ewes during pregnancy and lactation. The animals bore or were suckling twins. The experimental treatments included 2 levels of energy intake during 7 weeks prepartum (high : H ; low : B) and 2 states of body reserves at 8 weeks prepartum (fat : G ; lean : M). Jugular blood samples were taken from ewes at 34, 20 and 6 days prepartum, and from lambs at lambing and at 6 hrs., 24 hrs., 12 days and 24 days after lambing. Blood glucose, urea and free amino acids and plasma insulin were recorded. Reducing dietary energy supply did not change lamb birth weight in autumn (fig. 1 A). It contrasted with a large diminution in the spring (Grizard et al., 1979a, b). There was a decrease of glucose and of some non-essential free amino acids (alanine, glutamate, proline, citrulline) in the blood of the ewes. Most of the non-essential free amino acids decreased during the first day of life in the blood of lambs (fig. 2A). Reducing body reserves resulted in an increase of glucose and glutamine but in a decrease of some other free amino acids (glutamate, ornithine, histidine) in the blood of ewes (fig. 1 B). It increased daily weight gain during the first 3 weeks of life in lambs (fig. 2B). At the beginning of life, plasma insulin in lambs seemed to be related to birthweight and to blood glucose in the mothers.
这项2 × 2因子试验(表1)于秋季对22只妊娠和哺乳期的利姆塞纳母羊进行。这些动物生了或正在吃奶的双胞胎。试验处理包括准备7周2个能量摄入水平(高:H;低:B)和2种状态的身体储备在8周前(脂肪:G;在母羊准备的第34天、第20天和第6天,以及羔羊在产羔和第6小时采集颈静脉血样。24小时。产羔后12天和24天。记录血糖、尿素、游离氨基酸和血浆胰岛素。减少饲粮能量供应并没有改变秋季羔羊的出生体重(图1a)。与之形成鲜明对比的是,春季羔羊的出生体重会大幅下降(griard等人,1979a, b)。母羊血液中的葡萄糖和一些非必需的游离氨基酸(丙氨酸、谷氨酸、脯氨酸、瓜氨酸)会下降。羔羊血液中的大多数非必需游离氨基酸在出生第一天就减少了(图2A)。机体储备的减少导致母羊血液中葡萄糖和谷氨酰胺的增加,但其他一些游离氨基酸(谷氨酸、鸟氨酸、组氨酸)的减少(图1b)。在羔羊出生后的前3周内,它增加了每日体重增加(图2B)。在生命之初,羔羊的血浆胰岛素似乎与出生体重和母亲的血糖有关。
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引用次数: 2
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Annales De Biologie Animale Biochimie Biophysique
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