Objective: Our research endeavors to discover innovative formulations for the pharmaceutical component of radiopharmaceuticals, which are used to diagnose Alzheimer's disease. Our approach involves the incorporation of Donepezil, a proven active ingredient, into lipid-based nanocarrier systems. Additionally, we have conducted a comprehensive study on the cytotoxicity of Donepezil as a vital aspect of our research. Material and Method: Two distinct techniques were employed in creating nanocarrier formulations: emulsion and sonication. Malvern Zeta Sizer measurements were conducted to assess the properties of the prepared formulations. In addition, the cell proliferation kit II (XTT) was used to evaluate the cytotoxicity of the active ingredient Donepezil. Result and Discussion: Formulations with particle sizes ranging from 100-200 nm have been selected based on the results of characterization studies. Cytotoxicity assays have shown that amounts of Donepezil (50, 100, 500, 1000, 2000, and 5000 µg/ml) are biocompatible. These findings confirm the optimal formulation parameters for producing high-quality Donepezil-based pharmaceutical products. The characterization studies of the prepared formulations have shown that they have the potential to be used in the diagnosis of Alzheimer's disease.
{"title":"PREPARATION OF LIPID NANOCARRIER FORMULATIONS AND CYTOTOXICITY STUDIES OF DONEPEZIL","authors":"E. Demi̇r, Emre Özgenç, E. Gündoğdu","doi":"10.33483/jfpau.1400158","DOIUrl":"https://doi.org/10.33483/jfpau.1400158","url":null,"abstract":"Objective: Our research endeavors to discover innovative formulations for the pharmaceutical component of radiopharmaceuticals, which are used to diagnose Alzheimer's disease. Our approach involves the incorporation of Donepezil, a proven active ingredient, into lipid-based nanocarrier systems. Additionally, we have conducted a comprehensive study on the cytotoxicity of Donepezil as a vital aspect of our research.\u0000Material and Method: Two distinct techniques were employed in creating nanocarrier formulations: emulsion and sonication. Malvern Zeta Sizer measurements were conducted to assess the properties of the prepared formulations. In addition, the cell proliferation kit II (XTT) was used to evaluate the cytotoxicity of the active ingredient Donepezil.\u0000Result and Discussion: Formulations with particle sizes ranging from 100-200 nm have been selected based on the results of characterization studies. Cytotoxicity assays have shown that amounts of Donepezil (50, 100, 500, 1000, 2000, and 5000 µg/ml) are biocompatible. These findings confirm the optimal formulation parameters for producing high-quality Donepezil-based pharmaceutical products. The characterization studies of the prepared formulations have shown that they have the potential to be used in the diagnosis of Alzheimer's disease.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"27 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140232881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study was conducted to systematically document the use of plants, plant parts and preparation methods used by people in eight districts and sixty villages in Ağrı province. Material and Method: The medicinal plant species employed by the indigenous population for therapeutic purposes were systematically gathered and identified. Comprehensive data on traditionally utilized information were compiled, and herbarium materials were prepared. These materials have been deposited at the Iğdır National Wild Life Museum (INWM), affiliated with Iğdır University. Result and Discussion: This research identified a total of 58 taxa of medicinal plants belonging to 31 families. Among these, 50 species were found to grow naturally, while 8 species were cultivated. The predominant plant families included Asteraceae (8), Apiaceae (4), Lamiaceae (4), and Rosaceae (4). Infusion emerged as the most widely employed preparation method. The practice of traditional medicine remained prevalent among the population in Ağrı. Nevertheless, with the increasing availability of health services in the region, herbal medicine appeared to be more closely associated with healthcare and illness prevention than with curative purposes. The influx of new immigrants also contributed to the erosion of traditional knowledge. Notably, there is a discernible decline in traditional knowledge regarding the use of medicinal plants, both among younger generations and due to migration. Furthermore, this research serves as a foundational resource for prospective scientific inquiries aimed at the development of novel commercial drugs derived from plant sources.
{"title":"AĞRI (TÜRKİYE) İLİ TIBBİ BİTKİLERİNİN ETNOBOTANİK ÇALIŞMASI: GELENEKSEL BİLGİ VE TEDAVİ POTANSİYELİNİN KEŞFEDİLMESİ","authors":"Belkıs Muca Yiğit, Sefa Gözcü","doi":"10.33483/jfpau.1402467","DOIUrl":"https://doi.org/10.33483/jfpau.1402467","url":null,"abstract":"Objective: This study was conducted to systematically document the use of plants, plant parts and preparation methods used by people in eight districts and sixty villages in Ağrı province.\u0000Material and Method: The medicinal plant species employed by the indigenous population for therapeutic purposes were systematically gathered and identified. Comprehensive data on traditionally utilized information were compiled, and herbarium materials were prepared. These materials have been deposited at the Iğdır National Wild Life Museum (INWM), affiliated with Iğdır University.\u0000Result and Discussion: This research identified a total of 58 taxa of medicinal plants belonging to 31 families. Among these, 50 species were found to grow naturally, while 8 species were cultivated. The predominant plant families included Asteraceae (8), Apiaceae (4), Lamiaceae (4), and Rosaceae (4). Infusion emerged as the most widely employed preparation method. The practice of traditional medicine remained prevalent among the population in Ağrı. Nevertheless, with the increasing availability of health services in the region, herbal medicine appeared to be more closely associated with healthcare and illness prevention than with curative purposes. The influx of new immigrants also contributed to the erosion of traditional knowledge. Notably, there is a discernible decline in traditional knowledge regarding the use of medicinal plants, both among younger generations and due to migration. Furthermore, this research serves as a foundational resource for prospective scientific inquiries aimed at the development of novel commercial drugs derived from plant sources.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"40 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140231594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aimed to reveal drug-drug interactions (DDIs) due to antacids through programs used to detect DDIs. Material and Method: Within the scope of this study, 207 prescriptions containing at least one antacid and a drug from a different pharmacological group were evaluated in terms of DDIs. Evaluations were made on the prescriptions received in a community pharmacy serving in Van, Türkiye. Three different DDI checking programs were used for this evaluation. Result and Discussion: Antacid-induced DDIs were detected in 64 of the prescriptions. Interactions occurred between 52 active ingredient pairs, and it was revealed that DDIs were most common between calcium carbonate and famotidine. This interaction is minor and has been detected by only one database. Another common interaction was found between the calcium carbonate and cholecalciferol (Vitamin D) pair, and this interaction was reported as Level 2 and should be closely monitored in two different databases. As a result, DDIs induced by antiacids generally were found to be at moderate levels. However, it is seen that three DDI checking programs used in the study provide different results in detecting DDIs.
{"title":"BİR TOPLUM ECZANESİNDE KARŞILAŞILAN ANTİASİTLER KAYNAKLI İLAÇ ETKİLEŞİMLERİNİN TESPİTİ: GÖZLEMSEL BİR ÇALIŞMA","authors":"Didem Gökeş, M. Arslan","doi":"10.33483/jfpau.1432068","DOIUrl":"https://doi.org/10.33483/jfpau.1432068","url":null,"abstract":"Objective: This study aimed to reveal drug-drug interactions (DDIs) due to antacids through programs used to detect DDIs. \u0000Material and Method: Within the scope of this study, 207 prescriptions containing at least one antacid and a drug from a different pharmacological group were evaluated in terms of DDIs. Evaluations were made on the prescriptions received in a community pharmacy serving in Van, Türkiye. Three different DDI checking programs were used for this evaluation.\u0000Result and Discussion: Antacid-induced DDIs were detected in 64 of the prescriptions. Interactions occurred between 52 active ingredient pairs, and it was revealed that DDIs were most common between calcium carbonate and famotidine. This interaction is minor and has been detected by only one database. Another common interaction was found between the calcium carbonate and cholecalciferol (Vitamin D) pair, and this interaction was reported as Level 2 and should be closely monitored in two different databases. As a result, DDIs induced by antiacids generally were found to be at moderate levels. However, it is seen that three DDI checking programs used in the study provide different results in detecting DDIs.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"90 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140234109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadire Özenver, Yiğit Erkmen, Filiz Boyali, Ö. Demi̇rezer
Objective: Breast cancer is one of the most prevalent cancer types worldwide. Antioxidant sources may prevent the occurrence of cancer. Natural sources rich in phenolics, thus, may provide alternate agents in the management of breast cancer. Rumex species are widely distributed in Turkish flora. Emerging evidence has pointed out the antitumoral property of Rumex species on a variety of cancer cells. In the present study, we propose to test the ethanolic extracts of the inner perianth segments of 14 Rumex species on four breast cancer cells with different origins. We also demonstrated their toxicity on healthy cells. Material and Method: We performed the resazurin reduction assay to examine the cytotoxicity and toxicity. Furthermore, we determined the phenolic contents of the extracts as an indicator of their antioxidant profile and ascertained their antioxidant activities by DPPH radical, ABTS radical cation scavenging activity and cupric ion-reducing antioxidant capacity assays. Result and Discussion: The ethanolic extracts of the inner perianth segments of Rumex species exhibited remarkable cytotoxicity profiles neither on breast cancer cells nor on healthy H9c2 rat myoblastoma cells. However, they usually displayed strong antioxidant activities due to possessing high phenolic content.
{"title":"CYTOTOXICITY SCREENING AND ANTIOXIDANT CAPACITY ASSESSMENT OF THE INNER PERIANTH SEGMENTS OF 14 RUMEX SPECIES GROWN IN TÜRKİYE","authors":"Nadire Özenver, Yiğit Erkmen, Filiz Boyali, Ö. Demi̇rezer","doi":"10.33483/jfpau.1391998","DOIUrl":"https://doi.org/10.33483/jfpau.1391998","url":null,"abstract":"Objective: Breast cancer is one of the most prevalent cancer types worldwide. Antioxidant sources may prevent the occurrence of cancer. Natural sources rich in phenolics, thus, may provide alternate agents in the management of breast cancer. Rumex species are widely distributed in Turkish flora. Emerging evidence has pointed out the antitumoral property of Rumex species on a variety of cancer cells. In the present study, we propose to test the ethanolic extracts of the inner perianth segments of 14 Rumex species on four breast cancer cells with different origins. We also demonstrated their toxicity on healthy cells. \u0000Material and Method: We performed the resazurin reduction assay to examine the cytotoxicity and toxicity. Furthermore, we determined the phenolic contents of the extracts as an indicator of their antioxidant profile and ascertained their antioxidant activities by DPPH radical, ABTS radical cation scavenging activity and cupric ion-reducing antioxidant capacity assays. \u0000Result and Discussion: The ethanolic extracts of the inner perianth segments of Rumex species exhibited remarkable cytotoxicity profiles neither on breast cancer cells nor on healthy H9c2 rat myoblastoma cells. However, they usually displayed strong antioxidant activities due to possessing high phenolic content.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"4 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140241932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Mucoadhesive polymers have emerged as crucial components in the realm of drug delivery systems, particularly in the context of targeted treatments within the colon. These polymers possess adhesive properties that enable them to form temporary bonds with mucosal surfaces, extending the contact time of drugs with the colonic mucosa. This review provides a comprehensive overview of mucoadhesive polymers for colon drug delivery systems. Natural polymers such as chitosan and alginate, along with synthetic counterparts like polyacrylic acid derivatives, find application in these systems. The advantages of mucoadhesive polymers lie in their ability to facilitate site-specific drug delivery, thereby minimizing systemic side effects, and in enabling controlled and sustained release of drugs for improved bioavailability. Despite these benefits, challenges including variability in mucosal conditions and the imperative need for biocompatibility must be addressed. The applications of mucoadhesive polymers span diverse medical conditions, including targeted delivery of anti-inflammatory drugs for inflammatory bowel diseases, localized administration of chemotherapeutic agents for colon cancer treatment, and precise delivery of antibiotics for colonic infections. Result and Discussion: As a promising avenue for optimizing colon drug delivery, mucoadhesive polymers offer great potential for the development of effective and well-tolerated treatments for various colonic disorders.
{"title":"MUKOADEZİF POLİMERLERİN KOLON HEDEFLİ İLAÇ TAŞIYICI SİSTEMLERDE KULLANIMI: DETAYLI BİR İNCELEME","authors":"Aylin DELJAVAN GHODRATI, Tansel Çomoğlu","doi":"10.33483/jfpau.1400920","DOIUrl":"https://doi.org/10.33483/jfpau.1400920","url":null,"abstract":"Objective: Mucoadhesive polymers have emerged as crucial components in the realm of drug delivery systems, particularly in the context of targeted treatments within the colon. These polymers possess adhesive properties that enable them to form temporary bonds with mucosal surfaces, extending the contact time of drugs with the colonic mucosa. This review provides a comprehensive overview of mucoadhesive polymers for colon drug delivery systems. Natural polymers such as chitosan and alginate, along with synthetic counterparts like polyacrylic acid derivatives, find application in these systems. The advantages of mucoadhesive polymers lie in their ability to facilitate site-specific drug delivery, thereby minimizing systemic side effects, and in enabling controlled and sustained release of drugs for improved bioavailability. Despite these benefits, challenges including variability in mucosal conditions and the imperative need for biocompatibility must be addressed. The applications of mucoadhesive polymers span diverse medical conditions, including targeted delivery of anti-inflammatory drugs for inflammatory bowel diseases, localized administration of chemotherapeutic agents for colon cancer treatment, and precise delivery of antibiotics for colonic infections.\u0000Result and Discussion: As a promising avenue for optimizing colon drug delivery, mucoadhesive polymers offer great potential for the development of effective and well-tolerated treatments for various colonic disorders.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"7 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140248579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aimed to carry out phytochemical profiling, antioxidant, antidiabetic, and ADMET study on the crude ethanol extract (CR) of Diospyros mespiliformis (DM) and its ethyl acetate (EEF) and aqueous fractions (AQF). Material and Method: The phytochemicals were identified by GC-MS. The antioxidant activity was determined in vitro and silico while the antidiabetic and ADMET were in silico. Result and Discussion: Exactly 54 and 44 compounds were respectively identified in the EEF and AQF. At 300 µg/ml, the CR demonstrated a significantly (p < 0.05) higher ascorbic acid equivalent (AAE) total antioxidant capacity (TAC) (73.59 ± 0.011 µg/ml) than the EEF (41.28 ± 0.003 µg/ml AAE) and AQF (31.28 ± 0.005 µg/ml AAE). The total reducing power (TRP) of the AQF (106.84 ± 3.46 µg/ml) was significantly (p <0.05) higher than the CR (93.23 ± 5.63 µg/ml AAE) and EEF (92.35 ± 6.96 µg/ml AAE) at 100 µg/ml. A significantly (p < 0.05) higher percentage inhibition (48.38% ± 4.61) was demonstrated by the EEF at 1 mg/ml in the ferric thiocyanate and a lower malonaldehyde concentration (0.75 ± 0.01 nmol/ml) in the thiobarbituric acid methods. The AQF demonstrated a significantly (p < 0.05) higher (82.72% ± 1.88) peroxide scavenging activity at 100 µg/ml than the CR (33.33% ± 2.16) and EEF (63.64% ± 2.66). Compound VII exhibited the lowest binding affinity (BA) and inhibition constant (Ki) of -8.8 kcal/mol and 0.35 µM, respectively with xanthine oxidase and -8.0 kcal/mol and 1.35 µM, respectively with NADH oxidase. X exhibited the lowest BA (-8.5 kcal/mol) and Ki (0.58 µM) interacting with CytP450 21A2. Compound III exhibited the lowest BA (-7.5 kcal/mol) and Ki (3.14 µM) with PTP1B while compound X had BA and Ki values of -8.5 kcal/mol and 0.58 µM, respectively with PPARγ. The result of ADMET showed some of the compounds might be strong candidates for antioxidant and antidiabetic drugs. All the extracts possess significant antioxidant activity and some of the identified compounds might be candidates for novel antioxidants and antidiabetic drugs.
{"title":"PHYTOCHEMICAL PROFILING, ANTIOXIDANT, ANTIDIABETIC, AND ADMET STUDY OF DIOSPYROS MESPILIFORMIS HOCHST. EX A. DC. (EBENACEAE) LEAF","authors":"M. Dahiru, Neksumi Musa","doi":"10.33483/jfpau.1354293","DOIUrl":"https://doi.org/10.33483/jfpau.1354293","url":null,"abstract":"Objective: This study aimed to carry out phytochemical profiling, antioxidant, antidiabetic, and ADMET study on the crude ethanol extract (CR) of Diospyros mespiliformis (DM) and its ethyl acetate (EEF) and aqueous fractions (AQF).\u0000Material and Method: The phytochemicals were identified by GC-MS. The antioxidant activity was determined in vitro and silico while the antidiabetic and ADMET were in silico. \u0000Result and Discussion: Exactly 54 and 44 compounds were respectively identified in the EEF and AQF. At 300 µg/ml, the CR demonstrated a significantly (p < 0.05) higher ascorbic acid equivalent (AAE) total antioxidant capacity (TAC) (73.59 ± 0.011 µg/ml) than the EEF (41.28 ± 0.003 µg/ml AAE) and AQF (31.28 ± 0.005 µg/ml AAE). The total reducing power (TRP) of the AQF (106.84 ± 3.46 µg/ml) was significantly (p <0.05) higher than the CR (93.23 ± 5.63 µg/ml AAE) and EEF (92.35 ± 6.96 µg/ml AAE) at 100 µg/ml. A significantly (p < 0.05) higher percentage inhibition (48.38% ± 4.61) was demonstrated by the EEF at 1 mg/ml in the ferric thiocyanate and a lower malonaldehyde concentration (0.75 ± 0.01 nmol/ml) in the thiobarbituric acid methods. The AQF demonstrated a significantly (p < 0.05) higher (82.72% ± 1.88) peroxide scavenging activity at 100 µg/ml than the CR (33.33% ± 2.16) and EEF (63.64% ± 2.66). Compound VII exhibited the lowest binding affinity (BA) and inhibition constant (Ki) of -8.8 kcal/mol and 0.35 µM, respectively with xanthine oxidase and -8.0 kcal/mol and 1.35 µM, respectively with NADH oxidase. X exhibited the lowest BA (-8.5 kcal/mol) and Ki (0.58 µM) interacting with CytP450 21A2. Compound III exhibited the lowest BA (-7.5 kcal/mol) and Ki (3.14 µM) with PTP1B while compound X had BA and Ki values of -8.5 kcal/mol and 0.58 µM, respectively with PPARγ. The result of ADMET showed some of the compounds might be strong candidates for antioxidant and antidiabetic drugs. All the extracts possess significant antioxidant activity and some of the identified compounds might be candidates for novel antioxidants and antidiabetic drugs.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139848508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aimed to carry out phytochemical profiling, antioxidant, antidiabetic, and ADMET study on the crude ethanol extract (CR) of Diospyros mespiliformis (DM) and its ethyl acetate (EEF) and aqueous fractions (AQF). Material and Method: The phytochemicals were identified by GC-MS. The antioxidant activity was determined in vitro and silico while the antidiabetic and ADMET were in silico. Result and Discussion: Exactly 54 and 44 compounds were respectively identified in the EEF and AQF. At 300 µg/ml, the CR demonstrated a significantly (p < 0.05) higher ascorbic acid equivalent (AAE) total antioxidant capacity (TAC) (73.59 ± 0.011 µg/ml) than the EEF (41.28 ± 0.003 µg/ml AAE) and AQF (31.28 ± 0.005 µg/ml AAE). The total reducing power (TRP) of the AQF (106.84 ± 3.46 µg/ml) was significantly (p <0.05) higher than the CR (93.23 ± 5.63 µg/ml AAE) and EEF (92.35 ± 6.96 µg/ml AAE) at 100 µg/ml. A significantly (p < 0.05) higher percentage inhibition (48.38% ± 4.61) was demonstrated by the EEF at 1 mg/ml in the ferric thiocyanate and a lower malonaldehyde concentration (0.75 ± 0.01 nmol/ml) in the thiobarbituric acid methods. The AQF demonstrated a significantly (p < 0.05) higher (82.72% ± 1.88) peroxide scavenging activity at 100 µg/ml than the CR (33.33% ± 2.16) and EEF (63.64% ± 2.66). Compound VII exhibited the lowest binding affinity (BA) and inhibition constant (Ki) of -8.8 kcal/mol and 0.35 µM, respectively with xanthine oxidase and -8.0 kcal/mol and 1.35 µM, respectively with NADH oxidase. X exhibited the lowest BA (-8.5 kcal/mol) and Ki (0.58 µM) interacting with CytP450 21A2. Compound III exhibited the lowest BA (-7.5 kcal/mol) and Ki (3.14 µM) with PTP1B while compound X had BA and Ki values of -8.5 kcal/mol and 0.58 µM, respectively with PPARγ. The result of ADMET showed some of the compounds might be strong candidates for antioxidant and antidiabetic drugs. All the extracts possess significant antioxidant activity and some of the identified compounds might be candidates for novel antioxidants and antidiabetic drugs.
{"title":"PHYTOCHEMICAL PROFILING, ANTIOXIDANT, ANTIDIABETIC, AND ADMET STUDY OF DIOSPYROS MESPILIFORMIS HOCHST. EX A. DC. (EBENACEAE) LEAF","authors":"M. Dahiru, Neksumi Musa","doi":"10.33483/jfpau.1354293","DOIUrl":"https://doi.org/10.33483/jfpau.1354293","url":null,"abstract":"Objective: This study aimed to carry out phytochemical profiling, antioxidant, antidiabetic, and ADMET study on the crude ethanol extract (CR) of Diospyros mespiliformis (DM) and its ethyl acetate (EEF) and aqueous fractions (AQF).\u0000Material and Method: The phytochemicals were identified by GC-MS. The antioxidant activity was determined in vitro and silico while the antidiabetic and ADMET were in silico. \u0000Result and Discussion: Exactly 54 and 44 compounds were respectively identified in the EEF and AQF. At 300 µg/ml, the CR demonstrated a significantly (p < 0.05) higher ascorbic acid equivalent (AAE) total antioxidant capacity (TAC) (73.59 ± 0.011 µg/ml) than the EEF (41.28 ± 0.003 µg/ml AAE) and AQF (31.28 ± 0.005 µg/ml AAE). The total reducing power (TRP) of the AQF (106.84 ± 3.46 µg/ml) was significantly (p <0.05) higher than the CR (93.23 ± 5.63 µg/ml AAE) and EEF (92.35 ± 6.96 µg/ml AAE) at 100 µg/ml. A significantly (p < 0.05) higher percentage inhibition (48.38% ± 4.61) was demonstrated by the EEF at 1 mg/ml in the ferric thiocyanate and a lower malonaldehyde concentration (0.75 ± 0.01 nmol/ml) in the thiobarbituric acid methods. The AQF demonstrated a significantly (p < 0.05) higher (82.72% ± 1.88) peroxide scavenging activity at 100 µg/ml than the CR (33.33% ± 2.16) and EEF (63.64% ± 2.66). Compound VII exhibited the lowest binding affinity (BA) and inhibition constant (Ki) of -8.8 kcal/mol and 0.35 µM, respectively with xanthine oxidase and -8.0 kcal/mol and 1.35 µM, respectively with NADH oxidase. X exhibited the lowest BA (-8.5 kcal/mol) and Ki (0.58 µM) interacting with CytP450 21A2. Compound III exhibited the lowest BA (-7.5 kcal/mol) and Ki (3.14 µM) with PTP1B while compound X had BA and Ki values of -8.5 kcal/mol and 0.58 µM, respectively with PPARγ. The result of ADMET showed some of the compounds might be strong candidates for antioxidant and antidiabetic drugs. All the extracts possess significant antioxidant activity and some of the identified compounds might be candidates for novel antioxidants and antidiabetic drugs.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139788372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Maranta arundinacea L. (Ararot) (Marantaceae) tropiklerde yetişen otsu, çok yıllık bir bitkidir. Yüksek nişasta içerikli yenilebilir silindirik rizomlara sahiptir. Rizomlar halk tıbbında yatıştırıcı, kızarıklık giderici, anti-inflamatuvar ve antiseptik olarak kullanılmaktadır. Bu çalışmada günümüzde ekonomik değeri olan ve çeşitli endüstrilerde uygulama potansiyeli bulunan ararotun botanik özellikleri, yayılışı ve geleneksel kullanımı araştırılmıştır. Dahası yenilebilir rizomlarının kimyasal içeriği ve rizomlardan elde edilen ekstre, un, nişasta ve tozun biyolojik aktiviteleri ve rizomların çeşitli kullanım alanlarına ait bilgiler derlenmiştir. Sonuç ve Tartışma: Rizomlar alkaloit, karbonhidrat, kardiyak glikozitler, protein, amino asit, terpen, saponin, flavonoit, reçine, tanen, zamk, lignin, antrakinon, sterol, lif ve mineral içerir. Rizom ve rizomdan elde edilen ürünlerin antioksidan, immünostimulan, anti-ülserojenik, antidiyareik, anti-inflamatuvar, antimikrobiyal, antidispeptik, antihipertansif, hipokolesterolemik, hipoglisemik, antikanser ve hepatoprotektif aktiviteleri bildirilmiştir. Ararot unu kolayca sindirilebildiğinden çocuklar için hazırlanan ekmek ve bisküvilerde ve ayrıca kurabiye ve unlu mamullerde kullanılır. Ararot nişastası; çorba, tatlılar, puding, sos, kurabiye, şekerleme, bisküvi, kek ve jöle yapımında kullanılır. Ararot nişastası glüten içermediğinden özel bisküvi ve fırın ürünlerinin hazırlanmasında kullanılır. Ararot tozu ve nişastasının kozmetik endüstrisinde kullanımı vardır. Ararot nişastasının eczacılık, hijyen ürünleri, çevre yönetimi, tarım, biyofilm, biyomedikal mühendisliği ve biyoyakıt üretimi gibi alanlarda uygulamaları mevcuttur. Ararot lifi kâğıt mendil, ince kâğıt, karton, ambalaj kâğıdı ve çanta gibi yırtılmaya dirençli kâğıt yapımına uygundur.
{"title":"ARAROT (MARANTA ARUNDINACEA L.) RİZOMLARININ FİTOKİMYASAL, TIBBİ VE BESİNSEL ÖZELLİKLERİ VE ÇEŞİTLİ KULLANIMLARI","authors":"Kübra Öğüt, Sevda GÜZEL KARA","doi":"10.33483/jfpau.1392485","DOIUrl":"https://doi.org/10.33483/jfpau.1392485","url":null,"abstract":"Amaç: Maranta arundinacea L. (Ararot) (Marantaceae) tropiklerde yetişen otsu, çok yıllık bir bitkidir. Yüksek nişasta içerikli yenilebilir silindirik rizomlara sahiptir. Rizomlar halk tıbbında yatıştırıcı, kızarıklık giderici, anti-inflamatuvar ve antiseptik olarak kullanılmaktadır. Bu çalışmada günümüzde ekonomik değeri olan ve çeşitli endüstrilerde uygulama potansiyeli bulunan ararotun botanik özellikleri, yayılışı ve geleneksel kullanımı araştırılmıştır. Dahası yenilebilir rizomlarının kimyasal içeriği ve rizomlardan elde edilen ekstre, un, nişasta ve tozun biyolojik aktiviteleri ve rizomların çeşitli kullanım alanlarına ait bilgiler derlenmiştir.\u0000Sonuç ve Tartışma: Rizomlar alkaloit, karbonhidrat, kardiyak glikozitler, protein, amino asit, terpen, saponin, flavonoit, reçine, tanen, zamk, lignin, antrakinon, sterol, lif ve mineral içerir. Rizom ve rizomdan elde edilen ürünlerin antioksidan, immünostimulan, anti-ülserojenik, antidiyareik, anti-inflamatuvar, antimikrobiyal, antidispeptik, antihipertansif, hipokolesterolemik, hipoglisemik, antikanser ve hepatoprotektif aktiviteleri bildirilmiştir. Ararot unu kolayca sindirilebildiğinden çocuklar için hazırlanan ekmek ve bisküvilerde ve ayrıca kurabiye ve unlu mamullerde kullanılır. Ararot nişastası; çorba, tatlılar, puding, sos, kurabiye, şekerleme, bisküvi, kek ve jöle yapımında kullanılır. Ararot nişastası glüten içermediğinden özel bisküvi ve fırın ürünlerinin hazırlanmasında kullanılır. Ararot tozu ve nişastasının kozmetik endüstrisinde kullanımı vardır. Ararot nişastasının eczacılık, hijyen ürünleri, çevre yönetimi, tarım, biyofilm, biyomedikal mühendisliği ve biyoyakıt üretimi gibi alanlarda uygulamaları mevcuttur. Ararot lifi kâğıt mendil, ince kâğıt, karton, ambalaj kâğıdı ve çanta gibi yırtılmaya dirençli kâğıt yapımına uygundur.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"17 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139856283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Maranta arundinacea L. (Ararot) (Marantaceae) tropiklerde yetişen otsu, çok yıllık bir bitkidir. Yüksek nişasta içerikli yenilebilir silindirik rizomlara sahiptir. Rizomlar halk tıbbında yatıştırıcı, kızarıklık giderici, anti-inflamatuvar ve antiseptik olarak kullanılmaktadır. Bu çalışmada günümüzde ekonomik değeri olan ve çeşitli endüstrilerde uygulama potansiyeli bulunan ararotun botanik özellikleri, yayılışı ve geleneksel kullanımı araştırılmıştır. Dahası yenilebilir rizomlarının kimyasal içeriği ve rizomlardan elde edilen ekstre, un, nişasta ve tozun biyolojik aktiviteleri ve rizomların çeşitli kullanım alanlarına ait bilgiler derlenmiştir. Sonuç ve Tartışma: Rizomlar alkaloit, karbonhidrat, kardiyak glikozitler, protein, amino asit, terpen, saponin, flavonoit, reçine, tanen, zamk, lignin, antrakinon, sterol, lif ve mineral içerir. Rizom ve rizomdan elde edilen ürünlerin antioksidan, immünostimulan, anti-ülserojenik, antidiyareik, anti-inflamatuvar, antimikrobiyal, antidispeptik, antihipertansif, hipokolesterolemik, hipoglisemik, antikanser ve hepatoprotektif aktiviteleri bildirilmiştir. Ararot unu kolayca sindirilebildiğinden çocuklar için hazırlanan ekmek ve bisküvilerde ve ayrıca kurabiye ve unlu mamullerde kullanılır. Ararot nişastası; çorba, tatlılar, puding, sos, kurabiye, şekerleme, bisküvi, kek ve jöle yapımında kullanılır. Ararot nişastası glüten içermediğinden özel bisküvi ve fırın ürünlerinin hazırlanmasında kullanılır. Ararot tozu ve nişastasının kozmetik endüstrisinde kullanımı vardır. Ararot nişastasının eczacılık, hijyen ürünleri, çevre yönetimi, tarım, biyofilm, biyomedikal mühendisliği ve biyoyakıt üretimi gibi alanlarda uygulamaları mevcuttur. Ararot lifi kâğıt mendil, ince kâğıt, karton, ambalaj kâğıdı ve çanta gibi yırtılmaya dirençli kâğıt yapımına uygundur.
{"title":"ARAROT (MARANTA ARUNDINACEA L.) RİZOMLARININ FİTOKİMYASAL, TIBBİ VE BESİNSEL ÖZELLİKLERİ VE ÇEŞİTLİ KULLANIMLARI","authors":"Kübra Öğüt, Sevda GÜZEL KARA","doi":"10.33483/jfpau.1392485","DOIUrl":"https://doi.org/10.33483/jfpau.1392485","url":null,"abstract":"Amaç: Maranta arundinacea L. (Ararot) (Marantaceae) tropiklerde yetişen otsu, çok yıllık bir bitkidir. Yüksek nişasta içerikli yenilebilir silindirik rizomlara sahiptir. Rizomlar halk tıbbında yatıştırıcı, kızarıklık giderici, anti-inflamatuvar ve antiseptik olarak kullanılmaktadır. Bu çalışmada günümüzde ekonomik değeri olan ve çeşitli endüstrilerde uygulama potansiyeli bulunan ararotun botanik özellikleri, yayılışı ve geleneksel kullanımı araştırılmıştır. Dahası yenilebilir rizomlarının kimyasal içeriği ve rizomlardan elde edilen ekstre, un, nişasta ve tozun biyolojik aktiviteleri ve rizomların çeşitli kullanım alanlarına ait bilgiler derlenmiştir.\u0000Sonuç ve Tartışma: Rizomlar alkaloit, karbonhidrat, kardiyak glikozitler, protein, amino asit, terpen, saponin, flavonoit, reçine, tanen, zamk, lignin, antrakinon, sterol, lif ve mineral içerir. Rizom ve rizomdan elde edilen ürünlerin antioksidan, immünostimulan, anti-ülserojenik, antidiyareik, anti-inflamatuvar, antimikrobiyal, antidispeptik, antihipertansif, hipokolesterolemik, hipoglisemik, antikanser ve hepatoprotektif aktiviteleri bildirilmiştir. Ararot unu kolayca sindirilebildiğinden çocuklar için hazırlanan ekmek ve bisküvilerde ve ayrıca kurabiye ve unlu mamullerde kullanılır. Ararot nişastası; çorba, tatlılar, puding, sos, kurabiye, şekerleme, bisküvi, kek ve jöle yapımında kullanılır. Ararot nişastası glüten içermediğinden özel bisküvi ve fırın ürünlerinin hazırlanmasında kullanılır. Ararot tozu ve nişastasının kozmetik endüstrisinde kullanımı vardır. Ararot nişastasının eczacılık, hijyen ürünleri, çevre yönetimi, tarım, biyofilm, biyomedikal mühendisliği ve biyoyakıt üretimi gibi alanlarda uygulamaları mevcuttur. Ararot lifi kâğıt mendil, ince kâğıt, karton, ambalaj kâğıdı ve çanta gibi yırtılmaya dirençli kâğıt yapımına uygundur.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"8 3‐4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139796492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Lamiaceae family has a wide variety of well-known and lesser-known plants with strong medicinal qualities. The genus Micromeria Benth. is a member of this family consisting of herbaceous plants with a variety of significant biological, phytochemical, and ethnobotanical uses. In this study, the biological activities of methanol and ethanol extracts of Micromeria myrtifolia were evaluated. Material and Method: To demonstrate the antioxidant activity DPPH radical scavenging activity and total phenolic content assays were done. The effects of the extracts on acetylcholinesterase (AChE) and monoamine oxidase-A were then assessed. Result and Discussion: Methanol extract showed the highest DPPH scavenging activity, at the dose of 10 mg/ml with a value of 96.55%. For the highest concentration that can be applicable, AChE inhibitions for the methanol and ethanol extracts were 25% and 27%, respectively. On the other hand, the inhibitory effects of the ethanol and methanol extracts of the plant on MAO-A were determined; for the ethanol extract IC50 value was found as 32.5876 ± 0.89 g/ml, and for the methanol extract it was found as 34.6544 ± 0.76 g/ml. It can be told that M. myrtifolia can act as a potential antioxidant. With further research and investigation, it is thought that Micromeria myrtifolia could be used as a natural source for the treatment of various neurological diseases.
{"title":"MICROMERIA MYRTIFOLIA BOISS. & HOHEN’İN BİYOLOJİK AKTİVİTELERİNİN BELİRLENMESİ","authors":"Y. Yücel, Ebru ÖZDEMİR NATH","doi":"10.33483/jfpau.1372535","DOIUrl":"https://doi.org/10.33483/jfpau.1372535","url":null,"abstract":"Objective: Lamiaceae family has a wide variety of well-known and lesser-known plants with strong medicinal qualities. The genus Micromeria Benth. is a member of this family consisting of herbaceous plants with a variety of significant biological, phytochemical, and ethnobotanical uses. In this study, the biological activities of methanol and ethanol extracts of Micromeria myrtifolia were evaluated.\u0000Material and Method: To demonstrate the antioxidant activity DPPH radical scavenging activity and total phenolic content assays were done. The effects of the extracts on acetylcholinesterase (AChE) and monoamine oxidase-A were then assessed. \u0000Result and Discussion: Methanol extract showed the highest DPPH scavenging activity, at the dose of 10 mg/ml with a value of 96.55%. For the highest concentration that can be applicable, AChE inhibitions for the methanol and ethanol extracts were 25% and 27%, respectively. On the other hand, the inhibitory effects of the ethanol and methanol extracts of the plant on MAO-A were determined; for the ethanol extract IC50 value was found as 32.5876 ± 0.89 g/ml, and for the methanol extract it was found as 34.6544 ± 0.76 g/ml. It can be told that M. myrtifolia can act as a potential antioxidant. With further research and investigation, it is thought that Micromeria myrtifolia could be used as a natural source for the treatment of various neurological diseases.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"38 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139864171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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