Objective: The present study aimed to assess ethical sensitivity in second, third, fourth, and fifth-year pharmacy students who have already completed the ethics course in Erzurum province, Turkey, as well as first-year pharmacy students who have not yet taken the ethics course in terms of various variables. Material and Method: This study adopts a cross-sectional design to compare the ethical sensitivity of two groups of pharmacy students: those who have completed an ethics course and those who have not. This study included 476 out of 840 undergraduate pharmacy students from Erzurum province. Differences in ethical sensitivity based on demographic characteristics were analyzed using t-tests and ANOVA. The level of statistical significance was set at 0.05. Result and Discussion: In the comparison between students who have taken the ethics course and those who have not, a statistically significant difference was found between female and male students who have taken the ethics course (p=0.00), indicating that gender plays a role in ethical sensitivity among students who have received ethics education. However, among students who have not taken the ethics course, no statistically significant difference was found between genders (p=0.13). No statistically significant difference was observed among students in different university classes. In the study assessing professional ethical sensitivity among pharmacy students, it was found that their level of ethical sensitivity was above average. To cultivate a heightened level of professional ethical sensitivity, it is widely believed that pharmacy education should integrate more comprehensive training focused on emphasizing the significance of professional behavior and ethical values.
{"title":"ETHICAL SENSITIVITY: A COMPARATIVE ANALYSIS OF PHARMACY STUDENTS TAKING AND NOT TAKING AN ETHICS COURSE IN TERMS OF VARIOUS VARIABLES","authors":"Elif Ulutaş Deniz, Rumeysa Eren","doi":"10.33483/jfpau.1315012","DOIUrl":"https://doi.org/10.33483/jfpau.1315012","url":null,"abstract":"Objective: The present study aimed to assess ethical sensitivity in second, third, fourth, and fifth-year pharmacy students who have already completed the ethics course in Erzurum province, Turkey, as well as first-year pharmacy students who have not yet taken the ethics course in terms of various variables.\u0000Material and Method: This study adopts a cross-sectional design to compare the ethical sensitivity of two groups of pharmacy students: those who have completed an ethics course and those who have not. This study included 476 out of 840 undergraduate pharmacy students from Erzurum province. Differences in ethical sensitivity based on demographic characteristics were analyzed using t-tests and ANOVA. The level of statistical significance was set at 0.05.\u0000Result and Discussion: In the comparison between students who have taken the ethics course and those who have not, a statistically significant difference was found between female and male students who have taken the ethics course (p=0.00), indicating that gender plays a role in ethical sensitivity among students who have received ethics education. However, among students who have not taken the ethics course, no statistically significant difference was found between genders (p=0.13). No statistically significant difference was observed among students in different university classes. In the study assessing professional ethical sensitivity among pharmacy students, it was found that their level of ethical sensitivity was above average. To cultivate a heightened level of professional ethical sensitivity, it is widely believed that pharmacy education should integrate more comprehensive training focused on emphasizing the significance of professional behavior and ethical values.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46353183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Ergüç, Ege Arzuk, Gökay Albayrak, Fuat Karakuş, Hayati Okur, Şüra Baykan
Objective: The first goal of the present study is to investigate the role of mitochondria due to the Crabtree effect in HepG2 cells exposed to ISO in either glucose- or galactose-conditioned media. The second aim is to predict the interactions between electron transport chain (ETC) complexes and ISO, which might be the possible reason for mitochondrial dysfunction. Material and Method: Cell viability and membrane damage for HepG2 cells exposed to ISO (12.5, 25, 50, 100, and 250 µM) were assessed by MTT and LDH leakage assays in either glucose- or galactose-conditioned media. The affinity of ISO to ETC complexes was also determined by a molecular docking study. Result and Discussion: MTT assay showed that 250 µM ISO leads to cytotoxic activity in glucose-conditioned media, while 25 µM and higher concentrations of ISO decrease cell viability in galactose-conditioned media. A membrane damage assay conducted in a glucose-conditioned media assay revealed that 250 µM ISO disrupts the cell membrane. 100 and 250 µM ISO increased membrane damage in galactose-conditioned media. According to docking simulations, binding affinities of ISO to ETC complexes are in descending order: Complex IV > Complex I > Complex III > Complex II. Inhibition of complex IV by ISO inhibits the transfer of electrons from cytochrome c to oxygen, and the proton gradient collapses. The present study proposed that ISO leads to mitochondrial dysfunction via inhibition of the ETC.
{"title":"İZOİMPERATORİN ARACILIKLI ANTİKANSER AKTİVİTE: MİTOKONDRİYAL DİSFONKSİYONUN HEPG2 HÜCRELERİNDEKİ ROLÜ","authors":"Ali Ergüç, Ege Arzuk, Gökay Albayrak, Fuat Karakuş, Hayati Okur, Şüra Baykan","doi":"10.33483/jfpau.1312637","DOIUrl":"https://doi.org/10.33483/jfpau.1312637","url":null,"abstract":"Objective: The first goal of the present study is to investigate the role of mitochondria due to the Crabtree effect in HepG2 cells exposed to ISO in either glucose- or galactose-conditioned media. The second aim is to predict the interactions between electron transport chain (ETC) complexes and ISO, which might be the possible reason for mitochondrial dysfunction.\u0000Material and Method: Cell viability and membrane damage for HepG2 cells exposed to ISO (12.5, 25, 50, 100, and 250 µM) were assessed by MTT and LDH leakage assays in either glucose- or galactose-conditioned media. The affinity of ISO to ETC complexes was also determined by a molecular docking study.\u0000Result and Discussion: MTT assay showed that 250 µM ISO leads to cytotoxic activity in glucose-conditioned media, while 25 µM and higher concentrations of ISO decrease cell viability in galactose-conditioned media. A membrane damage assay conducted in a glucose-conditioned media assay revealed that 250 µM ISO disrupts the cell membrane. 100 and 250 µM ISO increased membrane damage in galactose-conditioned media. According to docking simulations, binding affinities of ISO to ETC complexes are in descending order: Complex IV > Complex I > Complex III > Complex II. Inhibition of complex IV by ISO inhibits the transfer of electrons from cytochrome c to oxygen, and the proton gradient collapses. The present study proposed that ISO leads to mitochondrial dysfunction via inhibition of the ETC.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42684108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, it was aimed to investigate the anatomical and palynological features of endemic Thymus brachychilus Jalas. Material and Method: The plant material was fixed in 70% alcohol for anatomical investigation. Cross sections of the stem, leaves, and leaf surface sections were stained with sartur solution, and the anatomical aspects were examined. The trichome architectures, mesophyll cells, stomatal index, and glandular and non-glandular trichomes features of leaves and stem have been investigated and compared with the other Thymus species anatomical findings. In addition, measurements of anatomical structures were made. Pollen, flower parts, epidermis and trichome structure of the leaves, were examined in SEM. Result and Discussion: This is the first anatomical, palynological and micromorphological study on Thymus brachychilus. The epidermis of leaf consists of a single row of elongated shaped cells and is covered with a thin cuticle. There are nonglandular and glandular trichomes in the upper and lower epidermis. Two different types of nonglandular trichomes were found. Stomata are diacytic, and the epidermis is amphistomatic. The mesophyll layer is dorsiventral. The stem cross-section is square. The outermost single-row, elongated, square or rectangular epidermis is covered with a thin cuticle. The pollen symmetry is isopolar, shape is suboblate. It has hexacolpate and bireticulate properties.
{"title":"ANATOMICAL, PALYNOLOGICAL AND MICROMOPHOLOGICAL INVESTIGATIONS ON ENDEMIC THYMUS BRACHYCHILUS JALAS (LAMIACEAE)","authors":"Ebru Özdemi̇r, Sefa Sönmez","doi":"10.33483/jfpau.1296874","DOIUrl":"https://doi.org/10.33483/jfpau.1296874","url":null,"abstract":"Objective: In this study, it was aimed to investigate the anatomical and palynological features of endemic Thymus brachychilus Jalas. \u0000Material and Method: The plant material was fixed in 70% alcohol for anatomical investigation. Cross sections of the stem, leaves, and leaf surface sections were stained with sartur solution, and the anatomical aspects were examined. The trichome architectures, mesophyll cells, stomatal index, and glandular and non-glandular trichomes features of leaves and stem have been investigated and compared with the other Thymus species anatomical findings. In addition, measurements of anatomical structures were made. Pollen, flower parts, epidermis and trichome structure of the leaves, were examined in SEM. \u0000Result and Discussion: This is the first anatomical, palynological and micromorphological study on Thymus brachychilus. The epidermis of leaf consists of a single row of elongated shaped cells and is covered with a thin cuticle. There are nonglandular and glandular trichomes in the upper and lower epidermis. Two different types of nonglandular trichomes were found. Stomata are diacytic, and the epidermis is amphistomatic. The mesophyll layer is dorsiventral. The stem cross-section is square. The outermost single-row, elongated, square or rectangular epidermis is covered with a thin cuticle. The pollen symmetry is isopolar, shape is suboblate. It has hexacolpate and bireticulate properties.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46849160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Esra Köngül Şafak, Selen İlgün, Kübra Nur Çoban, Sena AKÇAKAYA MUTLU, H. Yilmaz, Gökçe Şeker Karatoprak
Objective: The present study aimed to evaluate the biological activities of Myrtus communis L., which has traditional medicinal use for different purposes, from a broad perspective. Material and Method: The antioxidant (DPPH, ABTS, total phenol, and flavonoid amounts) anti-inflammatory (LOX enzyme inhibition), antidiabetic (α-glucosidase enzyme inhibition), and cytotoxic properties (MTT assay) of a 70% methanol extract made from leaves and dichloromethane, butanol, ethyl acetate, and the residual water fractions were investigated. Result and Discussion: The DPPH radical scavenging effect of the ethyl acetate fraction, with the highest total phenol and flavonoid content, was found to be 1.4 µg/ml, and the ABTS radical scavenging effect was 2.58 mmol/Trolox. The most potent inhibitor of LOX (IC50: 31.17 µg/ml) and α-glucosidase (96% inhibition at 0.5 mg/ml) enzymes was determined as ethyl acetate extract. Dichloromethane fraction was shown to have the most cytotoxic activity in both Hela and Colo cell lines. This research has given us a better understanding of the traditional use of the M. communis plant, which stands out for its therapeutic properties.
目的:对具有多种传统药用价值的桃金娘(Myrtus communis L.)进行生物活性评价。材料与方法:研究了70%甲醇提取液与二氯甲烷、丁醇、乙酸乙酯和残水组分的抗氧化(DPPH、ABTS、总酚和类黄酮含量)、抗炎(LOX酶抑制)、抗糖尿病(α-葡萄糖苷酶抑制)和细胞毒性(MTT法)。结果与讨论:总酚和类黄酮含量最高的乙酸乙酯部位对DPPH自由基的清除作用为1.4µg/ml，对ABTS自由基的清除作用为2.58 mmol/Trolox。乙酸乙酯提取物对LOX酶(IC50: 31.17µg/ml)和α-葡萄糖苷酶(0.5 mg/ml抑制96%)的抑制作用最强。二氯甲烷馏分对Hela和Colo细胞株均具有最大的细胞毒活性。这项研究使我们更好地了解了M. communis植物的传统用途，它以其治疗特性而脱颖而出。
{"title":"ANTIOXIDANT, ENZYME INHIBITORY, AND CYTOTOXIC ACTIVITY SCREENING OF MYRTUS COMMUNIS L.","authors":"Esra Köngül Şafak, Selen İlgün, Kübra Nur Çoban, Sena AKÇAKAYA MUTLU, H. Yilmaz, Gökçe Şeker Karatoprak","doi":"10.33483/jfpau.1264321","DOIUrl":"https://doi.org/10.33483/jfpau.1264321","url":null,"abstract":"Objective: The present study aimed to evaluate the biological activities of Myrtus communis L., which has traditional medicinal use for different purposes, from a broad perspective.\u0000Material and Method: The antioxidant (DPPH, ABTS, total phenol, and flavonoid amounts) anti-inflammatory (LOX enzyme inhibition), antidiabetic (α-glucosidase enzyme inhibition), and cytotoxic properties (MTT assay) of a 70% methanol extract made from leaves and dichloromethane, butanol, ethyl acetate, and the residual water fractions were investigated.\u0000Result and Discussion: The DPPH radical scavenging effect of the ethyl acetate fraction, with the highest total phenol and flavonoid content, was found to be 1.4 µg/ml, and the ABTS radical scavenging effect was 2.58 mmol/Trolox. The most potent inhibitor of LOX (IC50: 31.17 µg/ml) and α-glucosidase (96% inhibition at 0.5 mg/ml) enzymes was determined as ethyl acetate extract. Dichloromethane fraction was shown to have the most cytotoxic activity in both Hela and Colo cell lines. This research has given us a better understanding of the traditional use of the M. communis plant, which stands out for its therapeutic properties.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44667246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Many substances have been used for wound-burn treatment to date. Recent studies have focused not only on covering the wound but also on the production of dressings that will provide patient comfort at the highest level. This review mentions the types of dressings and the expected features of an ideal wound dressing. An ideal wound dressing should close the wound and protect against external factors such as microorganisms. It must have an appropriate absorption capacity for the exudate level in terms of not drying the wound. It should mimic healthy skin with mechanical strength and flexibility. Wound dressing is expected to accelerate healing by supporting cell proliferation and migration. Result and Discussion: One or more of these features come to the fore when the patient's age and chronic diseases, wound depth, degree, and stage are considered. As a result, since many factors are influential in wound dressing selection, it is crucial to examine the properties of materials. Wound dressings should be developed by considering tissue debridement, infection control, moisture balance, and epithelization.
{"title":"İDEAL YARA ÖRTÜSÜNÜN ÖZELLİKLERİ","authors":"Seçil Kaya, Serap Derman","doi":"10.33483/jfpau.1253376","DOIUrl":"https://doi.org/10.33483/jfpau.1253376","url":null,"abstract":"Objective: Many substances have been used for wound-burn treatment to date. Recent studies have focused not only on covering the wound but also on the production of dressings that will provide patient comfort at the highest level. This review mentions the types of dressings and the expected features of an ideal wound dressing. An ideal wound dressing should close the wound and protect against external factors such as microorganisms. It must have an appropriate absorption capacity for the exudate level in terms of not drying the wound. It should mimic healthy skin with mechanical strength and flexibility. Wound dressing is expected to accelerate healing by supporting cell proliferation and migration.\u0000Result and Discussion: One or more of these features come to the fore when the patient's age and chronic diseases, wound depth, degree, and stage are considered. As a result, since many factors are influential in wound dressing selection, it is crucial to examine the properties of materials. Wound dressings should be developed by considering tissue debridement, infection control, moisture balance, and epithelization.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44222178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The main purpose of this study is to determine the effect of the tea brewing time on the total color, brightness, and fluoride ion concentration of tea infusions in Türkiye. Material and Method: 250 ml of boiling distilled water was added to 6 g of granulated tea sample and brewed against increasing time. The total color and brightness of the tea infusions were determined by a spectrophotometer at 460 nm, while a potentiometric method was used to determine the fluoride ion concentration. Result and Discussion: We conclude that the concentration of fluoride ions and other bioactive compounds in the tea infusion is strongly dependent on the tea preparation and brewing preferences of consumers. According to our results, fluoride concentrations in tea infusions reach the plateau after 20 minutes in a Turkish way of tea brewing. Therefore, tea-brewing time is an important variable in calculating the daily fluoride intake for extreme tea-consuming populations like Türkiye.
{"title":"TÜRKİYE’DE DEMLENMİŞ SİYAH ÇAYIN RENK VE PARLAKLIĞININ FLORÜR KONSANTRASYONUNA ETKİSİ","authors":"Üzeyir Aktuğ, Yalçın Duydu","doi":"10.33483/jfpau.1298235","DOIUrl":"https://doi.org/10.33483/jfpau.1298235","url":null,"abstract":"Objective: The main purpose of this study is to determine the effect of the tea brewing time on the total color, brightness, and fluoride ion concentration of tea infusions in Türkiye.\u0000Material and Method: 250 ml of boiling distilled water was added to 6 g of granulated tea sample and brewed against increasing time. The total color and brightness of the tea infusions were determined by a spectrophotometer at 460 nm, while a potentiometric method was used to determine the fluoride ion concentration. \u0000Result and Discussion: We conclude that the concentration of fluoride ions and other bioactive compounds in the tea infusion is strongly dependent on the tea preparation and brewing preferences of consumers. According to our results, fluoride concentrations in tea infusions reach the plateau after 20 minutes in a Turkish way of tea brewing. Therefore, tea-brewing time is an important variable in calculating the daily fluoride intake for extreme tea-consuming populations like Türkiye.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44099990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The purpose of the present work was to develop and validate a fast, simple and sensitive extraction-free spectrophotometric technique for the quantitative determination of meloxicam based on the reaction with bromothymol blue. Material and Method: The reference standard of meloxicam, bromothymol blue and finished dosage forms of meloxicam were used in the study. Absorption measurements were performed on the Analytic Jena UV-visible spectrophotometer model Specord 200. Result and Discussion: The developed method is based on the formation of the colored reaction product between meloxicam and bromothymol blue in acetone medium with absorption maximum at 348 nm. The method meets the requirements of the State Pharmacopoeia of Ukraine for such validation characteristics as specificity, linearity, precision, accuracy, robustness and range of application. The obedience to Beer's law is observed in the range of meloxicam concentrations 0.80-2.40 mg/100 ml, the correlation coefficient is 0.9998. The range of application of the method is 60-140%.
{"title":"EXTRACTION-FREE SPECTROPHOTOMETRIC DETERMINATION OF MELOXICAM USING BROMOTHYMOL BLUE","authors":"A. Donchenko, S. Vasyuk, N. Nahorna","doi":"10.33483/jfpau.1240900","DOIUrl":"https://doi.org/10.33483/jfpau.1240900","url":null,"abstract":"Objective: The purpose of the present work was to develop and validate a fast, simple and sensitive extraction-free spectrophotometric technique for the quantitative determination of meloxicam based on the reaction with bromothymol blue.\u0000Material and Method: The reference standard of meloxicam, bromothymol blue and finished dosage forms of meloxicam were used in the study. Absorption measurements were performed on the Analytic Jena UV-visible spectrophotometer model Specord 200.\u0000Result and Discussion: The developed method is based on the formation of the colored reaction product between meloxicam and bromothymol blue in acetone medium with absorption maximum at 348 nm. The method meets the requirements of the State Pharmacopoeia of Ukraine for such validation characteristics as specificity, linearity, precision, accuracy, robustness and range of application. The obedience to Beer's law is observed in the range of meloxicam concentrations 0.80-2.40 mg/100 ml, the correlation coefficient is 0.9998. The range of application of the method is 60-140%.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44557626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Zerumbone (ZER) is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential to inhibit Thymidine Phosphorylase (TP) and the ability to trigger Reactive oxygen species (ROS)-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, cell line. Material and Method: The antiangiogenic activity for ZER was evaluated by using the thymidine phosphorylase inhibitory test. Reactive oxygen species (ROS) production was determined via DCFDA dye by using flow cytometry. Result and Discussion: ZER was found to be potent TP inhibitory with the IC50 value of 50.3± 0.31 μg/ml or 230±1.42 µM. NCI-H460 cells upon treatment with ZER produced significant ROS by 55.7%. Consequently, ZER exerts anti-angiogenic properties and modulates ROS production in lung cancer cells, serving as leads for better therapeutic index in cancer drug.
{"title":"ANTIANGIOGENIC ACTIVITY AND ROS-MEDIATED LUNG CANCER CELL LINE INJURY OF ZERUMBONE","authors":"Shaymaa Fadhel ABBAS ALBAAYİT, M. K. Mohammed","doi":"10.33483/jfpau.1112778","DOIUrl":"https://doi.org/10.33483/jfpau.1112778","url":null,"abstract":"Objective: Zerumbone (ZER) is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential to inhibit Thymidine Phosphorylase (TP) and the ability to trigger Reactive oxygen species (ROS)-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, cell line. \u0000Material and Method: The antiangiogenic activity for ZER was evaluated by using the thymidine phosphorylase inhibitory test. Reactive oxygen species (ROS) production was determined via DCFDA dye by using flow cytometry. \u0000Result and Discussion: ZER was found to be potent TP inhibitory with the IC50 value of 50.3± 0.31 μg/ml or 230±1.42 µM. NCI-H460 cells upon treatment with ZER produced significant ROS by 55.7%. Consequently, ZER exerts anti-angiogenic properties and modulates ROS production in lung cancer cells, serving as leads for better therapeutic index in cancer drug.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47473349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Doğan, F. Mohammed, I. Uysal, K. Mencik, E. Kına, M. Pehlivan, M. Sevindik
Objective: Plants are preferred for biological effect. It is a natural resource used in the field of alternative medicine due to its biological effect. In our study, the total oxidant status (TOS) and oxidative stress index (OSI) and total antioxidant status (TAS) of Viola odorata L. species were detected. In addition, antimicrobial and antiproliferative effect of species was detected. Material and Method: The some parts of the species were used with the help of a soxhlet equipment, and ethanol was preferred as a solvent. TOS, OSI and TAS capacitiy were detected using Rel Assay kits. Agar dilution method was preferred to determine antimicrobial effect against bacteria and fungi. Lung cancer cell line (A549) was used to find out the antiproliferative effect by MTT assay. Result and Discussion: Consequently, the studies, the TAS capacitiy of V. odorata extract was detected as 6.752±0.139, the TOS capacitiy as 7.886±0.224 and the OSI capacitiy as 0.117±0.001. V. odorata extracts were determined to be influential against standard bacteria at 25-100 μg/ml intensiy and against fungi at 100-200 μg/ml intensiy. It was detected that the antiproliferative effect of V. odorata extract increased depending on the extract intensiy and showed strong effects. Consequently, it has been detected that V. odorata has important biological effects and in the pharmaceutical industry, it can be preferred after certain stages.
{"title":"TOTAL ANTIOXIDANT STATUS, ANTIMICROBIAL AND ANTIPROLIFERATIVE POTENTIALS OF VIOLA ODORATA (FRAGRANT VIOLET)","authors":"M. Doğan, F. Mohammed, I. Uysal, K. Mencik, E. Kına, M. Pehlivan, M. Sevindik","doi":"10.33483/jfpau.1161440","DOIUrl":"https://doi.org/10.33483/jfpau.1161440","url":null,"abstract":"Objective: Plants are preferred for biological effect. It is a natural resource used in the field of alternative medicine due to its biological effect. In our study, the total oxidant status (TOS) and oxidative stress index (OSI) and total antioxidant status (TAS) of Viola odorata L. species were detected. In addition, antimicrobial and antiproliferative effect of species was detected.\u0000Material and Method: The some parts of the species were used with the help of a soxhlet equipment, and ethanol was preferred as a solvent. TOS, OSI and TAS capacitiy were detected using Rel Assay kits. Agar dilution method was preferred to determine antimicrobial effect against bacteria and fungi. Lung cancer cell line (A549) was used to find out the antiproliferative effect by MTT assay.\u0000Result and Discussion: Consequently, the studies, the TAS capacitiy of V. odorata extract was detected as 6.752±0.139, the TOS capacitiy as 7.886±0.224 and the OSI capacitiy as 0.117±0.001. V. odorata extracts were determined to be influential against standard bacteria at 25-100 μg/ml intensiy and against fungi at 100-200 μg/ml intensiy. It was detected that the antiproliferative effect of V. odorata extract increased depending on the extract intensiy and showed strong effects. Consequently, it has been detected that V. odorata has important biological effects and in the pharmaceutical industry, it can be preferred after certain stages.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45529762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: High alert medications (HAM) are medications commonly used in health care settings that are associated with significant harm when used in error. Improvement in the knowledge and practice of pharmacists is crucial to prevent and solve medication errors associated with HAM. This study aimed to assess the knowledge of pharmacy students about HAM. Material and Method: A cross-sectional study was conducted among pharmacy students (3rd, 4th and 5th-year students) through an online survey in Ankara, Türkiye between 1 May 2021 and 31 October 2021. A Turkish translated and validated version of the High Alert Medications Knowledge Questionnaire was used. Only a correct answer was given score of 1 among the knowledge questions. The common resources for HAM use in the practice were assessed. Result and Discussion: Among 124 students, 80.6% were female. The mean age (standard derivation ± SD) of the students was 23.3 ± 1.37 years. The percentages of the 3rd, 4th and 5th-year students were 51.6%, 16.1% and 32.3%, respectively. The mean ± SD score of the students was 6.8 ± 3.28 out of 20. The 5th-year students (8.00 ± 2.90) were more likely to get higher scores compared to 3rd-years (5.89 ± 3.15) (p = 0.003). RxMediaPharma® (local online drug information database) was the most (75.0%) commonly used resource by pharmacy students. The pharmacy students’ knowledge about HAM was poor. Educational interventions are necessary early in the clinical pharmacy curriculum to improve pharmacy students' knowledge and preparedness to handle HAM.
{"title":"ECZACILIK ÖĞRENCİLERİNİN YÜKSEK RİSKLİ İLAÇLARIN KULLANIMI HAKKINDA BİLGİSİNİN DEĞERLENDİRİLMESİ","authors":"Aysu Selçuk, Betul Okuyan","doi":"10.33483/jfpau.1263375","DOIUrl":"https://doi.org/10.33483/jfpau.1263375","url":null,"abstract":"Objective: High alert medications (HAM) are medications commonly used in health care settings that are associated with significant harm when used in error. Improvement in the knowledge and practice of pharmacists is crucial to prevent and solve medication errors associated with HAM. This study aimed to assess the knowledge of pharmacy students about HAM.\u0000Material and Method: A cross-sectional study was conducted among pharmacy students (3rd, 4th and 5th-year students) through an online survey in Ankara, Türkiye between 1 May 2021 and 31 October 2021. A Turkish translated and validated version of the High Alert Medications Knowledge Questionnaire was used. Only a correct answer was given score of 1 among the knowledge questions. The common resources for HAM use in the practice were assessed. \u0000Result and Discussion: Among 124 students, 80.6% were female. The mean age (standard derivation ± SD) of the students was 23.3 ± 1.37 years. The percentages of the 3rd, 4th and 5th-year students were 51.6%, 16.1% and 32.3%, respectively. The mean ± SD score of the students was 6.8 ± 3.28 out of 20. The 5th-year students (8.00 ± 2.90) were more likely to get higher scores compared to 3rd-years (5.89 ± 3.15) (p = 0.003). RxMediaPharma® (local online drug information database) was the most (75.0%) commonly used resource by pharmacy students. The pharmacy students’ knowledge about HAM was poor. Educational interventions are necessary early in the clinical pharmacy curriculum to improve pharmacy students' knowledge and preparedness to handle HAM.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46814301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：