Objective: The objective of this study was to delve into the effect of N-acetylcysteine (NAC) molecule on the minimum inhibitory concentration (MIC) of meropenem, ciprofloxacin, and gentamicin in clinical isolates of Acinetobacter baumannii, aiming to find potential alternatives for the treatment of bacterial infections that are resistant to conventional antibiotics. Material and Method: The study included 25 A. baumannii isolates that were confirmed to be resistant to meropenem, ciprofloxacin, and gentamicin. The susceptibility of antibiotics was re-evaluated in the presence of NAC using the microdilution method. FIC indexes were calculated based on the checkerboard test results to determine the effect of the combination, defined as synergistic or additive. Result and Discussion: The study demonstrated that NAC molecule, when used alongside meropenem, ciprofloxacin, and gentamicin, effectively reduced the MIC values of these antibiotics against Acinetobacteria. Furthermore, NAC molecule exhibited a synergistic effect when combined with meropenem. Additive effects were observed in all isolates for the GEN-NAC and CIP-NAC combinations. In conclusion, the findings suggest that NAC molecule could serve as a new alternative for combined drug therapy, offering a promising approach to treatment.
{"title":"N-ASETİL SİSTEİNİN ACINETOBACTER BAUMANNII İZOLATLARINA KARŞI ANTİMİKROBİYAL ETKİSİ VE ANTİBİYOTİKLERLE ETKİLEŞİMİ","authors":"Alparslan Semih Salan, Suzan Ökten","doi":"10.33483/jfpau.1358483","DOIUrl":"https://doi.org/10.33483/jfpau.1358483","url":null,"abstract":"Objective: The objective of this study was to delve into the effect of N-acetylcysteine (NAC) molecule on the minimum inhibitory concentration (MIC) of meropenem, ciprofloxacin, and gentamicin in clinical isolates of Acinetobacter baumannii, aiming to find potential alternatives for the treatment of bacterial infections that are resistant to conventional antibiotics.\u0000Material and Method: The study included 25 A. baumannii isolates that were confirmed to be resistant to meropenem, ciprofloxacin, and gentamicin. The susceptibility of antibiotics was re-evaluated in the presence of NAC using the microdilution method. FIC indexes were calculated based on the checkerboard test results to determine the effect of the combination, defined as synergistic or additive.\u0000Result and Discussion: The study demonstrated that NAC molecule, when used alongside meropenem, ciprofloxacin, and gentamicin, effectively reduced the MIC values of these antibiotics against Acinetobacteria. Furthermore, NAC molecule exhibited a synergistic effect when combined with meropenem. Additive effects were observed in all isolates for the GEN-NAC and CIP-NAC combinations. In conclusion, the findings suggest that NAC molecule could serve as a new alternative for combined drug therapy, offering a promising approach to treatment.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"27 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139863820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The objective of this study was to delve into the effect of N-acetylcysteine (NAC) molecule on the minimum inhibitory concentration (MIC) of meropenem, ciprofloxacin, and gentamicin in clinical isolates of Acinetobacter baumannii, aiming to find potential alternatives for the treatment of bacterial infections that are resistant to conventional antibiotics. Material and Method: The study included 25 A. baumannii isolates that were confirmed to be resistant to meropenem, ciprofloxacin, and gentamicin. The susceptibility of antibiotics was re-evaluated in the presence of NAC using the microdilution method. FIC indexes were calculated based on the checkerboard test results to determine the effect of the combination, defined as synergistic or additive. Result and Discussion: The study demonstrated that NAC molecule, when used alongside meropenem, ciprofloxacin, and gentamicin, effectively reduced the MIC values of these antibiotics against Acinetobacteria. Furthermore, NAC molecule exhibited a synergistic effect when combined with meropenem. Additive effects were observed in all isolates for the GEN-NAC and CIP-NAC combinations. In conclusion, the findings suggest that NAC molecule could serve as a new alternative for combined drug therapy, offering a promising approach to treatment.
{"title":"N-ASETİL SİSTEİNİN ACINETOBACTER BAUMANNII İZOLATLARINA KARŞI ANTİMİKROBİYAL ETKİSİ VE ANTİBİYOTİKLERLE ETKİLEŞİMİ","authors":"Alparslan Semih Salan, Suzan Ökten","doi":"10.33483/jfpau.1358483","DOIUrl":"https://doi.org/10.33483/jfpau.1358483","url":null,"abstract":"Objective: The objective of this study was to delve into the effect of N-acetylcysteine (NAC) molecule on the minimum inhibitory concentration (MIC) of meropenem, ciprofloxacin, and gentamicin in clinical isolates of Acinetobacter baumannii, aiming to find potential alternatives for the treatment of bacterial infections that are resistant to conventional antibiotics.\u0000Material and Method: The study included 25 A. baumannii isolates that were confirmed to be resistant to meropenem, ciprofloxacin, and gentamicin. The susceptibility of antibiotics was re-evaluated in the presence of NAC using the microdilution method. FIC indexes were calculated based on the checkerboard test results to determine the effect of the combination, defined as synergistic or additive.\u0000Result and Discussion: The study demonstrated that NAC molecule, when used alongside meropenem, ciprofloxacin, and gentamicin, effectively reduced the MIC values of these antibiotics against Acinetobacteria. Furthermore, NAC molecule exhibited a synergistic effect when combined with meropenem. Additive effects were observed in all isolates for the GEN-NAC and CIP-NAC combinations. In conclusion, the findings suggest that NAC molecule could serve as a new alternative for combined drug therapy, offering a promising approach to treatment.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139803964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Lamiaceae family has a wide variety of well-known and lesser-known plants with strong medicinal qualities. The genus Micromeria Benth. is a member of this family consisting of herbaceous plants with a variety of significant biological, phytochemical, and ethnobotanical uses. In this study, the biological activities of methanol and ethanol extracts of Micromeria myrtifolia were evaluated. Material and Method: To demonstrate the antioxidant activity DPPH radical scavenging activity and total phenolic content assays were done. The effects of the extracts on acetylcholinesterase (AChE) and monoamine oxidase-A were then assessed. Result and Discussion: Methanol extract showed the highest DPPH scavenging activity, at the dose of 10 mg/ml with a value of 96.55%. For the highest concentration that can be applicable, AChE inhibitions for the methanol and ethanol extracts were 25% and 27%, respectively. On the other hand, the inhibitory effects of the ethanol and methanol extracts of the plant on MAO-A were determined; for the ethanol extract IC50 value was found as 32.5876 ± 0.89 g/ml, and for the methanol extract it was found as 34.6544 ± 0.76 g/ml. It can be told that M. myrtifolia can act as a potential antioxidant. With further research and investigation, it is thought that Micromeria myrtifolia could be used as a natural source for the treatment of various neurological diseases.
{"title":"MICROMERIA MYRTIFOLIA BOISS. & HOHEN’İN BİYOLOJİK AKTİVİTELERİNİN BELİRLENMESİ","authors":"Y. Yücel, Ebru ÖZDEMİR NATH","doi":"10.33483/jfpau.1372535","DOIUrl":"https://doi.org/10.33483/jfpau.1372535","url":null,"abstract":"Objective: Lamiaceae family has a wide variety of well-known and lesser-known plants with strong medicinal qualities. The genus Micromeria Benth. is a member of this family consisting of herbaceous plants with a variety of significant biological, phytochemical, and ethnobotanical uses. In this study, the biological activities of methanol and ethanol extracts of Micromeria myrtifolia were evaluated.\u0000Material and Method: To demonstrate the antioxidant activity DPPH radical scavenging activity and total phenolic content assays were done. The effects of the extracts on acetylcholinesterase (AChE) and monoamine oxidase-A were then assessed. \u0000Result and Discussion: Methanol extract showed the highest DPPH scavenging activity, at the dose of 10 mg/ml with a value of 96.55%. For the highest concentration that can be applicable, AChE inhibitions for the methanol and ethanol extracts were 25% and 27%, respectively. On the other hand, the inhibitory effects of the ethanol and methanol extracts of the plant on MAO-A were determined; for the ethanol extract IC50 value was found as 32.5876 ± 0.89 g/ml, and for the methanol extract it was found as 34.6544 ± 0.76 g/ml. It can be told that M. myrtifolia can act as a potential antioxidant. With further research and investigation, it is thought that Micromeria myrtifolia could be used as a natural source for the treatment of various neurological diseases.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"30 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139804193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Bu çalışmada benzoksazolon/benzotiyazolon halkası taşıyan asetamit türevi 28 yeni bileşik sentez edilmiş ve Alzheimer hastalığına karşı etkileri in vitro olarak test edilmiştir. Gereç ve Yöntem: Sonuç bileşiklerin sentezinde kullanılan amin türevleri (5 ve 6), öncelikle ticari olarak mevcut 1,3-benzoksazol-2(3H)-on ve 1,3-benzotiyazol-2(3H)-on halkalarının metillenmesi, nitrik asit ile nitrolanması ve ardından kalay klorürle indirgenmesi ile sentez edilmiştir. Daha sonra, amin türevi 5 ve 6’nın bromoasetil bromür ile açilasyonundan hazırlanan ara ürünlerin (7 ve 8) uygun amin türevleri ile tepkimesinden sonuç bileşikler (9a-n ve 10a-n) elde edilmiştir. Sentezlenen bileşiklerin kimyasal yapıları spektroskopik yöntemler, HRMS ve elementel analiz ile aydınlatılmıştır. Tüm sonuç bileşiklerin modifiye Ellman yöntemiyle kolinesteraz inhibitör aktiviteleri belirlendikten sonra DPPH ve ORAC yöntemiyle antioksidan aktiviteleri ölçülmüştür. Son olarak sonuç bileşiklerin metal şelatör özellikleri tayin edilmiştir. Sonuç ve Tartışma: Yeni 2-sübstitüe-N-(3-metil-2-okso-2,3-dihidro-1,3-benzoksazol/1,3-benzotiyazol-6-il)asetamit türevi bileşikler sentez edilmiştir. Kolinesteraz inhibitör etkileri ve yapı aktivite ilişkileri belirlenmiştir. Asetilkolinesteraz (AKE) inhibisyonu için bileşik 10g’nin (IC50: 52.90 µM), butirilkolinesteraz BKE inhibisyonu için bileşik 10h’nin (IC50: 51.03 µM ) en yüksek aktiviteye sahip olduğu bulunmuştur. ORAC testi ile yapılan antioksidan aktivite tayininde ise yan zincirinde fenilpiperazin türevleri taşıyan bileşiklerin referans trolokstan daha yüksek antioksidan aktivite gösterdikleri bulunmuştur. Ayrıca sonuç bileşiklerin metal şelatör etkileri incelendiğinde büyük çoğunluğunun metal şelatör özellik taşıdığı belirlenmiştir.
{"title":"BAZI BENZOKSAZOL-2(3H)-ON/BENZOTİYAZOL-2(3H)-ON TÜREVLERİNİN SENTEZİ VE ALZHEİMER HASTALIĞINA KARŞI ETKİLERİNİN İNCELENMESİ","authors":"Merve Bardakkaya, Burcu Kiliç, Deniz Doğruer","doi":"10.33483/jfpau.1387244","DOIUrl":"https://doi.org/10.33483/jfpau.1387244","url":null,"abstract":"Amaç: Bu çalışmada benzoksazolon/benzotiyazolon halkası taşıyan asetamit türevi 28 yeni bileşik sentez edilmiş ve Alzheimer hastalığına karşı etkileri in vitro olarak test edilmiştir.\u0000Gereç ve Yöntem: Sonuç bileşiklerin sentezinde kullanılan amin türevleri (5 ve 6), öncelikle ticari olarak mevcut 1,3-benzoksazol-2(3H)-on ve 1,3-benzotiyazol-2(3H)-on halkalarının metillenmesi, nitrik asit ile nitrolanması ve ardından kalay klorürle indirgenmesi ile sentez edilmiştir. Daha sonra, amin türevi 5 ve 6’nın bromoasetil bromür ile açilasyonundan hazırlanan ara ürünlerin (7 ve 8) uygun amin türevleri ile tepkimesinden sonuç bileşikler (9a-n ve 10a-n) elde edilmiştir. Sentezlenen bileşiklerin kimyasal yapıları spektroskopik yöntemler, HRMS ve elementel analiz ile aydınlatılmıştır. Tüm sonuç bileşiklerin modifiye Ellman yöntemiyle kolinesteraz inhibitör aktiviteleri belirlendikten sonra DPPH ve ORAC yöntemiyle antioksidan aktiviteleri ölçülmüştür. Son olarak sonuç bileşiklerin metal şelatör özellikleri tayin edilmiştir. \u0000Sonuç ve Tartışma: Yeni 2-sübstitüe-N-(3-metil-2-okso-2,3-dihidro-1,3-benzoksazol/1,3-benzotiyazol-6-il)asetamit türevi bileşikler sentez edilmiştir. Kolinesteraz inhibitör etkileri ve yapı aktivite ilişkileri belirlenmiştir. Asetilkolinesteraz (AKE) inhibisyonu için bileşik 10g’nin (IC50: 52.90 µM), butirilkolinesteraz BKE inhibisyonu için bileşik 10h’nin (IC50: 51.03 µM ) en yüksek aktiviteye sahip olduğu bulunmuştur. ORAC testi ile yapılan antioksidan aktivite tayininde ise yan zincirinde fenilpiperazin türevleri taşıyan bileşiklerin referans trolokstan daha yüksek antioksidan aktivite gösterdikleri bulunmuştur. Ayrıca sonuç bileşiklerin metal şelatör etkileri incelendiğinde büyük çoğunluğunun metal şelatör özellik taşıdığı belirlenmiştir.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"69 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammed Tilahun Muhammed, Nesli Aksoy, Aybüke Krilmaz, Enise Türkmen
Objective: Diabetes mellitus is a metabolic disorder affecting hundreds of millions of people around the world. It is characterized by hyperglycemia caused by impaired glucose homeostasis that results from insufficient insulin production or insulin resistance. There are clinically available α-glucosidase inhibitor drugs that are used to decrease postprandial blood glucose level. However, these drugs have side effects that necessitated the discovery of new α-glucosidase inhibitors with less side effects and high potency. The interest in the use of natural products to deal with diabetes has been increasing. Therefore, the potential of natural α-glucosidase inhibitors to inhibit the enzyme was investigated through computational methods. Material and Method: The binding potential of selected natural α-glucosidase inhibitors was investigated through molecular docking. Thereafter, the stability of the complexes with the highest binding potential were assessed through molecular dynamics (MD) simulation. Result and Discussion: The molecular docking demonstrated that compound 2 had better binding potential than the standard drug, acarbose. Compound 7 had comparable binding potential to the standard drug. Furthermore, all the tested compounds exhibited a reasonable binding potential towards the enzyme but were weaker than the standard drug. The MD simulation demonstrated that compounds 2 and 7 gave complexes with similar stability to the standard drug. The overall computational results revealed that the natural inhibitors investigated had the ability to bind to the enzyme and formed stable complexes. Therefore, these compounds could be potential α-glucosidase inhibitors for clinical use. For this reason, further in vitro investigations on compounds with the highest binding potential is recommended.
{"title":"TOWARDS UNDERSTANDING NATURAL ALPHA-GLUCOSIDASE INHIBITORS: A COMPUTATIONAL STUDY","authors":"Muhammed Tilahun Muhammed, Nesli Aksoy, Aybüke Krilmaz, Enise Türkmen","doi":"10.33483/jfpau.1356028","DOIUrl":"https://doi.org/10.33483/jfpau.1356028","url":null,"abstract":"Objective: Diabetes mellitus is a metabolic disorder affecting hundreds of millions of people around the world. It is characterized by hyperglycemia caused by impaired glucose homeostasis that results from insufficient insulin production or insulin resistance. There are clinically available α-glucosidase inhibitor drugs that are used to decrease postprandial blood glucose level. However, these drugs have side effects that necessitated the discovery of new α-glucosidase inhibitors with less side effects and high potency. The interest in the use of natural products to deal with diabetes has been increasing. Therefore, the potential of natural α-glucosidase inhibitors to inhibit the enzyme was investigated through computational methods. \u0000Material and Method: The binding potential of selected natural α-glucosidase inhibitors was investigated through molecular docking. Thereafter, the stability of the complexes with the highest binding potential were assessed through molecular dynamics (MD) simulation. \u0000Result and Discussion: The molecular docking demonstrated that compound 2 had better binding potential than the standard drug, acarbose. Compound 7 had comparable binding potential to the standard drug. Furthermore, all the tested compounds exhibited a reasonable binding potential towards the enzyme but were weaker than the standard drug. The MD simulation demonstrated that compounds 2 and 7 gave complexes with similar stability to the standard drug. The overall computational results revealed that the natural inhibitors investigated had the ability to bind to the enzyme and formed stable complexes. Therefore, these compounds could be potential α-glucosidase inhibitors for clinical use. For this reason, further in vitro investigations on compounds with the highest binding potential is recommended.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"67 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139381505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Halk arasında doğal kaynakların güvenli olarak kabul edilmesi ve sentetik içerikli ürünlerden uzaklaşma isteği, uçucu yağ kullanımını son zamanlarda arttırmıştır. Fakat uçucu yağlar, içerisinde çok sayıda bileşik barındırdığından standardizasyon konusunda sıkıntılar yaşanmaktadır. Bu sebeple uçucu yağların kalitesini ve terapötik amaçla kullanılabilirliğini değerlendirmek için monograflardan yararlanılmaktadır. Yapılan çeşitli çalışmalar sonucunda nioli uçucu yağının; üst solunum yolu ve üriner sistem enfeksiyonlarına, cilt rahatsızlıklarına karşı kullanılabileceği ve antioksidan, antienflamatuvar, antiseptik, antifungal, antibakteriyel, antihelmintik, insektisidal, larvisidal ve akarisidal etkinliği tespit edilmiştir. Halk arasında ise soğuk algınlığı, influenza, öksürük, sinüzit, farenjit, rinit, romatizmal rahatsızlıklarda ve üriner sistem enfeksiyonlarında kullanılmaktadır. Bu etkinlikleri ve halk arasındaki kullanımından yola çıkılarak, piyasada bulunan bazı nioli uçucu yağ örneklerinin Türk Farmakopesi 2017’ye uygunluğunun değerlendirilmesi hedeflenmiştir. Gereç ve Yöntem: Çalışma kapsamında; 5 farklı markadan temin edilen nioli uçucu yağı örneklerinin Türk Farmakopesi’nde (2017) yer alan monografa göre analiz edilmiştir. Analizde, bağıl yoğunluk, kırılım imleci, optik çevirme değerleri tespit edilmiş; organoleptik kontrol, ince tabaka kromatografisi ve gaz kromatografisi analizleri yürütülmüştür. Sonuç ve Tartışma: Çalışma kapsamında yapılan farmakope analizi sonucunda, temin edilen beş nioli uçucu yağ numunesinden sadece bir tanesi farmakope standardına uygun olduğu bulunmuştur. Diğer nioli uçucu yağ numuneleri, farmakopede istenilen şartları karşılamamıştır.
{"title":"PİYASADAN TEMİN EDİLEN NİOLİ UÇUCU YAĞI ÖRNEKLERİNİN FARMAKOPE ANALİZİ","authors":"Burçin Ergene, Gizem Öztürk","doi":"10.33483/jfpau.1406371","DOIUrl":"https://doi.org/10.33483/jfpau.1406371","url":null,"abstract":"Amaç: Halk arasında doğal kaynakların güvenli olarak kabul edilmesi ve sentetik içerikli ürünlerden uzaklaşma isteği, uçucu yağ kullanımını son zamanlarda arttırmıştır. Fakat uçucu yağlar, içerisinde çok sayıda bileşik barındırdığından standardizasyon konusunda sıkıntılar yaşanmaktadır. Bu sebeple uçucu yağların kalitesini ve terapötik amaçla kullanılabilirliğini değerlendirmek için monograflardan yararlanılmaktadır. Yapılan çeşitli çalışmalar sonucunda nioli uçucu yağının; üst solunum yolu ve üriner sistem enfeksiyonlarına, cilt rahatsızlıklarına karşı kullanılabileceği ve antioksidan, antienflamatuvar, antiseptik, antifungal, antibakteriyel, antihelmintik, insektisidal, larvisidal ve akarisidal etkinliği tespit edilmiştir. Halk arasında ise soğuk algınlığı, influenza, öksürük, sinüzit, farenjit, rinit, romatizmal rahatsızlıklarda ve üriner sistem enfeksiyonlarında kullanılmaktadır. Bu etkinlikleri ve halk arasındaki kullanımından yola çıkılarak, piyasada bulunan bazı nioli uçucu yağ örneklerinin Türk Farmakopesi 2017’ye uygunluğunun değerlendirilmesi hedeflenmiştir. Gereç ve Yöntem: Çalışma kapsamında; 5 farklı markadan temin edilen nioli uçucu yağı örneklerinin Türk Farmakopesi’nde (2017) yer alan monografa göre analiz edilmiştir. Analizde, bağıl yoğunluk, kırılım imleci, optik çevirme değerleri tespit edilmiş; organoleptik kontrol, ince tabaka kromatografisi ve gaz kromatografisi analizleri yürütülmüştür. Sonuç ve Tartışma: Çalışma kapsamında yapılan farmakope analizi sonucunda, temin edilen beş nioli uçucu yağ numunesinden sadece bir tanesi farmakope standardına uygun olduğu bulunmuştur. Diğer nioli uçucu yağ numuneleri, farmakopede istenilen şartları karşılamamıştır.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"101 51","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139134988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Wastewater and environmental water resources are treated to eliminate pathogenic microorganisms but contamination is still a significant problem. In particular, the presence of contamination with Escherichia coli (E. coli) is an important indicator of fecal contamination. Due to increasing antimicrobial resistance and failures of new antimicrobial processes, interest in bacteriophages in pathogen control has increased. Therefore, in our study, phage-based bacteria control in environmental waters was investigated as a natural solution. Material and Method: In our study, E. coli and lytic bacteriophages specific to these E. coli were isolated from environmental water samples in Ankara. The lytic activities of the isolated phages were determined on environmental and clinical extended-spectrum β-lactamases E. coli isolates. Three phages with high lytic activity were selected, and the effectiveness of the single phage and their mixtures on E. coli contamination in water was tested. Result and Discussion: As a result of the study, 17 E. coli strains were isolated from 30 environmental water samples. Lytic bacteriophages in 30 different plaque structures were also isolated from water samples. The isolated phages were found to have lytic activity in the range of 32-70% on the tested bacteria. The effectiveness of three selected phages and their cocktail on E. coli contamination in water was measured at 6th and 24th. As a result, it was observed that the cocktail application reduced the number of host bacteria in the water below detectable limits, also provided a 5-log reduction in non-host test bacteria and maintained its effect for 24 hours. When the results are evaluated, it is thought that cocktail phage application will be an effective method against E. coli contamination in water.
{"title":"THE EFFECT OF ESCHERICHIA COLI BACTERIOPHAGE COCKTAIL ON BACTERIAL CONTAMINATION IN WATER","authors":"H. B. Erol, Aylin Keski̇n","doi":"10.33483/jfpau.1390191","DOIUrl":"https://doi.org/10.33483/jfpau.1390191","url":null,"abstract":"Objective: Wastewater and environmental water resources are treated to eliminate pathogenic microorganisms but contamination is still a significant problem. In particular, the presence of contamination with Escherichia coli (E. coli) is an important indicator of fecal contamination. Due to increasing antimicrobial resistance and failures of new antimicrobial processes, interest in bacteriophages in pathogen control has increased. Therefore, in our study, phage-based bacteria control in environmental waters was investigated as a natural solution. Material and Method: In our study, E. coli and lytic bacteriophages specific to these E. coli were isolated from environmental water samples in Ankara. The lytic activities of the isolated phages were determined on environmental and clinical extended-spectrum β-lactamases E. coli isolates. Three phages with high lytic activity were selected, and the effectiveness of the single phage and their mixtures on E. coli contamination in water was tested. Result and Discussion: As a result of the study, 17 E. coli strains were isolated from 30 environmental water samples. Lytic bacteriophages in 30 different plaque structures were also isolated from water samples. The isolated phages were found to have lytic activity in the range of 32-70% on the tested bacteria. The effectiveness of three selected phages and their cocktail on E. coli contamination in water was measured at 6th and 24th. As a result, it was observed that the cocktail application reduced the number of host bacteria in the water below detectable limits, also provided a 5-log reduction in non-host test bacteria and maintained its effect for 24 hours. When the results are evaluated, it is thought that cocktail phage application will be an effective method against E. coli contamination in water.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139144604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Atomoxetine (ATX) is a medication that is extensively used to treat attention deficit hyperactivity disorder in children, adolescents, and adults. The goal of this work was to create a speedy, easy, and sensitive ultra high performance liquid chromatographic method (UHPLC) for the measurement of atomoxetine in various medicinal plants. (Salvia officinalis L., Rosmarinus officinalis L., Melissa officinalis L., Ginkgo biloba L.). Material and Method: Prior to chromatographic separation, liquid-liquid extraction was applied, which is currently the preferred extraction technique due to its simple, fast and efficient procedure for sample preparation. The chromatographic separation was achieved by reversed phase C18 (5 μm × 4.6 mm × 150 mm) analytical column and a mobile phase consisting of monobasic potassium dihydrogen orthophosphate (pH=6.8) and acetonitrile (50:50 v/v) at flow rate of 0.8 ml/min and diode array detector (DAD) detecting at 215±2 nm. Result and Discussion: The envisioned method's linear behavior was tested in the 0.5-20 μg/ml range (r2=0.09990). In compliance with International Conference on Harmonisation (ICH) criteria, the method received validation by means of accuracy, precision, repeatability, specificity, robustness, and detection and quantification boundaries. LOD and LOQ values were determined as 0.16 and 0.5 μg/ml. RSD values for hourly and daily measurements are found to be below 2.5% for both assays. The proposed method can be used effectively for quantification of atomoxetine in medicinal and aromatic plants. The proposed analytical procedure represents an efficient method for the quantification and routinee analysis of atomoxetine in medicinal and aromatic plants.
{"title":"BAZI TIBBİ BİTKİLERDE ATOMOKSETİN TAYİNİ İÇİN YENİ BİR UHPLC-DAD YÖNTEMİNİN GELİŞTİRİLMESİ VE VALİDASYONU","authors":"Burhan Ceylan","doi":"10.33483/jfpau.1345130","DOIUrl":"https://doi.org/10.33483/jfpau.1345130","url":null,"abstract":"Objective: Atomoxetine (ATX) is a medication that is extensively used to treat attention deficit hyperactivity disorder in children, adolescents, and adults. The goal of this work was to create a speedy, easy, and sensitive ultra high performance liquid chromatographic method (UHPLC) for the measurement of atomoxetine in various medicinal plants. (Salvia officinalis L., Rosmarinus officinalis L., Melissa officinalis L., Ginkgo biloba L.). Material and Method: Prior to chromatographic separation, liquid-liquid extraction was applied, which is currently the preferred extraction technique due to its simple, fast and efficient procedure for sample preparation. The chromatographic separation was achieved by reversed phase C18 (5 μm × 4.6 mm × 150 mm) analytical column and a mobile phase consisting of monobasic potassium dihydrogen orthophosphate (pH=6.8) and acetonitrile (50:50 v/v) at flow rate of 0.8 ml/min and diode array detector (DAD) detecting at 215±2 nm. Result and Discussion: The envisioned method's linear behavior was tested in the 0.5-20 μg/ml range (r2=0.09990). In compliance with International Conference on Harmonisation (ICH) criteria, the method received validation by means of accuracy, precision, repeatability, specificity, robustness, and detection and quantification boundaries. LOD and LOQ values were determined as 0.16 and 0.5 μg/ml. RSD values for hourly and daily measurements are found to be below 2.5% for both assays. The proposed method can be used effectively for quantification of atomoxetine in medicinal and aromatic plants. The proposed analytical procedure represents an efficient method for the quantification and routinee analysis of atomoxetine in medicinal and aromatic plants.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"48 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Coenzyme Q10 (CoQ10) offers potential therapeutic benefits for ocular health, yet faces challenges of poor solubility and bioavailability when applied to the eye. This study aimed to enhance CoQ10 delivery using contact lenses by incorporating CoQ10-loaded polymeric micelles, using Pluronic F127 and solvent evaporation technique. Material and Method: Polymeric micelles encapsulating CoQ10 were produced via solvent evaporation with Pluronic F127. Commercial contact lenses were subsequently loaded with these micelles. Characterization of the loaded lenses included assessments of light transmittance, swelling behavior, and drug release profile under non-sink conditions, simulating the constraints of the ocular surface. Result and Discussion: The unloaded lenses exhibited a light transmittance of 91.78±3.29% and swelling percentage of 47.51±4.45% while micelle-loaded lenses demonstrated high light transmittance levels (95.31±0.80%), ensuring optical clarity. Swelling studies showed a slight increase in size to 48.1±4.4%. The lenses effectively encapsulated 403.6±21.8 µg of CoQ10. In vitro release profile exhibited controlled release over six hours, indicating potential for sustained drug delivery. These results highlight the feasibility of micelle-loaded contact lenses for efficient ocular drug delivery, warranting further exploration into their long-term effectiveness and safety.
{"title":"KOENZİM Q10 YÜKLÜ MİSEL İÇEREN KONTAKT LENSLERİN HAZIRLANMASI VE İN-VİTRO KARAKTERİZASYONU","authors":"Ahmet Doğan Ergi̇n","doi":"10.33483/jfpau.1400373","DOIUrl":"https://doi.org/10.33483/jfpau.1400373","url":null,"abstract":"Objective: Coenzyme Q10 (CoQ10) offers potential therapeutic benefits for ocular health, yet faces challenges of poor solubility and bioavailability when applied to the eye. This study aimed to enhance CoQ10 delivery using contact lenses by incorporating CoQ10-loaded polymeric micelles, using Pluronic F127 and solvent evaporation technique. Material and Method: Polymeric micelles encapsulating CoQ10 were produced via solvent evaporation with Pluronic F127. Commercial contact lenses were subsequently loaded with these micelles. Characterization of the loaded lenses included assessments of light transmittance, swelling behavior, and drug release profile under non-sink conditions, simulating the constraints of the ocular surface. Result and Discussion: The unloaded lenses exhibited a light transmittance of 91.78±3.29% and swelling percentage of 47.51±4.45% while micelle-loaded lenses demonstrated high light transmittance levels (95.31±0.80%), ensuring optical clarity. Swelling studies showed a slight increase in size to 48.1±4.4%. The lenses effectively encapsulated 403.6±21.8 µg of CoQ10. In vitro release profile exhibited controlled release over six hours, indicating potential for sustained drug delivery. These results highlight the feasibility of micelle-loaded contact lenses for efficient ocular drug delivery, warranting further exploration into their long-term effectiveness and safety.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Lavandula angustifolia L. (Lavender) is one of the most plants essential oils used in the cosmetic, food and biological activities. Kojic acid has been used in cosmetics for its whitening effect and pharmaceutical activity. The aim of this study to determine the composition of the Pharmacopoeia quality L. angustifolia essential oil (EO). Moreover, antimicrobial activities against skin pathogens and synergistic antibacterial activity were also examined of EO and kojic acid. Material and Method: In this work, chemical composition of the EO was defined. Linalyl acetate (43.3%) and linalool (38.6%) were determined as the major components by GC-MS and GC-FID, simultaneously. The antimicrobial activity was evaluated against Candida albicans, C. glabrata, Staphylococcus aureus and Salmonella typhimurium. Result and Discussion: EO and kojic acid showed weak antimicrobial effects. MIC values were determined as the EO 10 mg/ml and kojic acid 1.25 mg/ml against S. typhimurium. To assess the synergistic activity was evaluated by the checkerboard microdilution assay, EO was combined with kojic acid against S. typhimurium. Among the tested skin pathogen microorganisms, S. typhimurium was more sensitive to kojic acid. Therefore, synergic activity was investigated against S. typhimurium and found indifferent effect.
目的：薰衣草（Lavandula angustifolia L.）是用于化妆品、食品和生物活动最多的植物精油之一。曲酸具有美白效果和药物活性，已被用于化妆品中。本研究的目的是确定药典级薰衣草精油（EO）的成分。此外，还研究了 EO 和曲酸对皮肤病原体的抗菌活性和协同抗菌活性。材料和方法：在这项工作中，确定了环氧乙烷的化学成分。通过 GC-MS 和 GC-FID 同时测定了乙酸芳樟酯（43.3%）和芳樟醇（38.6%）的主要成分。对白色念珠菌、光滑念珠菌、金黄色葡萄球菌和鼠伤寒沙门氏菌的抗菌活性进行了评估。结果与讨论：环氧乙烷和曲酸显示出微弱的抗菌效果。对伤寒沙门氏菌的 MIC 值分别为环氧乙烷 10 毫克/毫升和曲酸 1.25 毫克/毫升。为了评估协同活性，采用了棋盘式微量稀释试验，将环氧乙烷与曲酸结合起来，共同对抗伤寒杆菌。在测试的皮肤病原微生物中，鼠伤寒杆菌对曲酸更为敏感。因此，对伤寒杆菌的协同活性进行了研究，发现效果并不明显。
{"title":"KOJİK ASİT İLE LAVANDULA ANGUSTIFOLIA L. UÇUCU YAĞININ ANTİMİKROBİYAL KOMBİNASYONU","authors":"Damla Kirci, Özge ÖZŞEN BATUR, B. Demi̇rci̇","doi":"10.33483/jfpau.1399176","DOIUrl":"https://doi.org/10.33483/jfpau.1399176","url":null,"abstract":"Objective: Lavandula angustifolia L. (Lavender) is one of the most plants essential oils used in the cosmetic, food and biological activities. Kojic acid has been used in cosmetics for its whitening effect and pharmaceutical activity. The aim of this study to determine the composition of the Pharmacopoeia quality L. angustifolia essential oil (EO). Moreover, antimicrobial activities against skin pathogens and synergistic antibacterial activity were also examined of EO and kojic acid. Material and Method: In this work, chemical composition of the EO was defined. Linalyl acetate (43.3%) and linalool (38.6%) were determined as the major components by GC-MS and GC-FID, simultaneously. The antimicrobial activity was evaluated against Candida albicans, C. glabrata, Staphylococcus aureus and Salmonella typhimurium. Result and Discussion: EO and kojic acid showed weak antimicrobial effects. MIC values were determined as the EO 10 mg/ml and kojic acid 1.25 mg/ml against S. typhimurium. To assess the synergistic activity was evaluated by the checkerboard microdilution assay, EO was combined with kojic acid against S. typhimurium. Among the tested skin pathogen microorganisms, S. typhimurium was more sensitive to kojic acid. Therefore, synergic activity was investigated against S. typhimurium and found indifferent effect.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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