. Sorghum peduncle length is one of the key agronomic traits in sorghum production and has a critical impact on sorghum mechanization for harvesting. In this study, an F2 segregating population was constructed with significantly different long-peduncle KY133B and short-peduncle KY123B as parents, and the sorghum peduncle length gene was preliminarily located using BSA-seq technology. The association analysis was performed by ED algorithm and SNP-index (or InDel-index) algorithm, and finally the sorghum peduncle length association region was targeted on chromosome 7 and 10. In this study, the BSA-seq technique was used to rapidly and efficiently locate key genetic segments of sorghum peduncle length, which laid the foundation for subsequent functional validation and molecular studies of peduncle length genes.
{"title":"BSA-SEQ-BASED METHOD FOR LOCATING KEY GENETIC SEGMENTS OF PEDUNCLE LENGTH IN BREWING DWARF SORGHUM [SORGHUM BICOLOR (L.) MOENCH]","authors":"G.D. YANG, J.H. LI, Z.Y. HU, Z.Y. HAO, B.S. SUN, C.S. LIU, Q. WANG, X.X. MENG, W. GUO","doi":"10.15666/aeer/2105_43134321","DOIUrl":"https://doi.org/10.15666/aeer/2105_43134321","url":null,"abstract":". Sorghum peduncle length is one of the key agronomic traits in sorghum production and has a critical impact on sorghum mechanization for harvesting. In this study, an F2 segregating population was constructed with significantly different long-peduncle KY133B and short-peduncle KY123B as parents, and the sorghum peduncle length gene was preliminarily located using BSA-seq technology. The association analysis was performed by ED algorithm and SNP-index (or InDel-index) algorithm, and finally the sorghum peduncle length association region was targeted on chromosome 7 and 10. In this study, the BSA-seq technique was used to rapidly and efficiently locate key genetic segments of sorghum peduncle length, which laid the foundation for subsequent functional validation and molecular studies of peduncle length genes.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136368156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2105_46154632
K. VELUSWAMY, V.M. CHOWDARY, B. PALANISAMY, V. DURAISAMY, T. THOMAS, B.P. BHASKAR
. The impact of land use and land cover changes (LULCC) is one of the major contributors to increasing greenhouse gas emissions to the atmosphere. At the terrestrial surface, the impact of LULCC is realized in altered hydrology. Conversion of cultivable lands into fallow lands severely affects crop production in agriculturally dominant basins such as the Vaigai River Basin (VRB) in Tamil Nadu, India. Considered as a granary of South Tamil Nadu, any LULCC in VRB results in uncertainty in food production. Therefore, in this study, Landsat images were used to evaluate changes in land use and MODIS NDVI images to estimate changes in browning and greening in VRB during 2001-2020. We also analyzed the rainfall and river discharge in the basin to understand the variations from 2001 to 2019 concerning LULCC. The results showed an increase of seventy-seven percent in fallow lands between 2010 and 2020 and a forty-one to fifty-nine percent increase in urban settlements between 2001 and 2020 in the basin. The impacts of LULCC were realized in monsoon rainfall with no change in river discharge in the lower Vaigai Basin. The study results will aid regulated land use planning and encourage further research on feedback between terrestrial and atmospheric water fluxes for ensuring food security.
{"title":"AN EXPLORATIVE ANALYSIS OF LAND USE LAND COVER CHANGES AND HYDROMETEOROLOGY OF THE VAIGAI RIVER BASIN, INDIA","authors":"K. VELUSWAMY, V.M. CHOWDARY, B. PALANISAMY, V. DURAISAMY, T. THOMAS, B.P. BHASKAR","doi":"10.15666/aeer/2105_46154632","DOIUrl":"https://doi.org/10.15666/aeer/2105_46154632","url":null,"abstract":". The impact of land use and land cover changes (LULCC) is one of the major contributors to increasing greenhouse gas emissions to the atmosphere. At the terrestrial surface, the impact of LULCC is realized in altered hydrology. Conversion of cultivable lands into fallow lands severely affects crop production in agriculturally dominant basins such as the Vaigai River Basin (VRB) in Tamil Nadu, India. Considered as a granary of South Tamil Nadu, any LULCC in VRB results in uncertainty in food production. Therefore, in this study, Landsat images were used to evaluate changes in land use and MODIS NDVI images to estimate changes in browning and greening in VRB during 2001-2020. We also analyzed the rainfall and river discharge in the basin to understand the variations from 2001 to 2019 concerning LULCC. The results showed an increase of seventy-seven percent in fallow lands between 2010 and 2020 and a forty-one to fifty-nine percent increase in urban settlements between 2001 and 2020 in the basin. The impacts of LULCC were realized in monsoon rainfall with no change in river discharge in the lower Vaigai Basin. The study results will aid regulated land use planning and encourage further research on feedback between terrestrial and atmospheric water fluxes for ensuring food security.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136371737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2101_623635
C. Hu, C.H. Xu, Guangzhao Hu, Z. Zhang, F. Li, Y. Xie
{"title":"EFFECT OF WATER LEVEL AND SUBMERGENCE TIME ON LEAF GROWTH, STOICHIOMETRY AND HOMEOSTASIS OF CAREX BREVICUSPIS","authors":"C. Hu, C.H. Xu, Guangzhao Hu, Z. Zhang, F. Li, Y. Xie","doi":"10.15666/aeer/2101_623635","DOIUrl":"https://doi.org/10.15666/aeer/2101_623635","url":null,"abstract":"","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67125480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2102_10431059
Y. Ünal, H. Uysal, A. Koca, M. Zenbilci, M. Ersin
. According to recent genetic studies, there are eight subspecies of leopards (Panthera pardus) all over the world. Among these subspecies, the Anatolian leopard (Panthera pardus tulliana) is distributed in the southern and western regions of Turkey . It is a terrestrial mammal subspecies of Anatolia that is important historically, culturally and biologically. To date, there have been no studies about the Anatolian leopard, which is at the top of the food chain and has the characteristics of an umbrella species, key species and flag species in conservation studies. It is known that until 1975, the Anatolian leopard had dispersed along the forests of Central Anatolia, Mediterranean and Aegean regions. Since then, no living records have been taken, with the exception of unconfirmed information from scientists, local people and hunters. With this study, for the first time in history, one living individual(s) of the Anatolian leopard ( P. p. tulliana ) was photographed and video recorded via camera trap method, in the Western Taurus Mountains of the Mediterranean region of Turkey.
{"title":"NEW RECORDS OF THE LIVING ANATOLIAN LEOPARD (PANTHERA PARDUS TULLIANA L., 1758) IN THE MEDITERRANEAN REGION OF TURKEY","authors":"Y. Ünal, H. Uysal, A. Koca, M. Zenbilci, M. Ersin","doi":"10.15666/aeer/2102_10431059","DOIUrl":"https://doi.org/10.15666/aeer/2102_10431059","url":null,"abstract":". According to recent genetic studies, there are eight subspecies of leopards (Panthera pardus) all over the world. Among these subspecies, the Anatolian leopard (Panthera pardus tulliana) is distributed in the southern and western regions of Turkey . It is a terrestrial mammal subspecies of Anatolia that is important historically, culturally and biologically. To date, there have been no studies about the Anatolian leopard, which is at the top of the food chain and has the characteristics of an umbrella species, key species and flag species in conservation studies. It is known that until 1975, the Anatolian leopard had dispersed along the forests of Central Anatolia, Mediterranean and Aegean regions. Since then, no living records have been taken, with the exception of unconfirmed information from scientists, local people and hunters. With this study, for the first time in history, one living individual(s) of the Anatolian leopard ( P. p. tulliana ) was photographed and video recorded via camera trap method, in the Western Taurus Mountains of the Mediterranean region of Turkey.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67126631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2102_15331547
N. Turfan
. The present study was carried out in order to reveal the effects of exogen melatonin (50MT: 50 µM - 100 MT: 100 µM) treatment on the growth rate characteristic and bioactive compound content variations of three spinach cultivars (Acosta-ACO; Anlani-ANL, and Matador-MTR, respectively) grown under cold conditions. While MTR was the richest variety in terms of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content, ANL was the richest variety in terms of Chl a to Chl b ratio, and xanthophyll content. Ascorbic acid and protein concentration were stimulated by MT in all three variants, but ANL had the highest value. While the proline content of cultivars decreased with only 50 MT in ACO when compared to the control, GB content decreased in MT doses. RWC increased with MT in ANL, whereas it caused to a decrease in RWC of 100 MT in ACO and 50 MT in MTR. MDA concentration was at a lower level and decreased only in ACO with 50 MT when compared to the control, while the H 2 O 2 concentration was higher at MT doses in all three species. MT treatment stimulated SOD activity in ACO and ANL and increased POD activity in all three variants. Considering the growth characteristics such as root/stem length, leaf characteristics, and biomass, exogenous MT doses had positive effects on all three cultivars. These findings suggest that foliar MT treatment can attenuate cold damage by enhancing the enzymatic and non-enzymatic antioxidant capacity of spinach seedlings, as well as the growth rate.
{"title":"THE EFFECTS OF EXOGENOUS MELATONIN APPLICATION ON GROWTH RATE PARAMETERS AND BIOACTIVE COMPOUNDS OF SOME SPINACH CULTIVARS (SPINACIA OLERACEA L.) GROWN UNDER WINTER CONDITIONS","authors":"N. Turfan","doi":"10.15666/aeer/2102_15331547","DOIUrl":"https://doi.org/10.15666/aeer/2102_15331547","url":null,"abstract":". The present study was carried out in order to reveal the effects of exogen melatonin (50MT: 50 µM - 100 MT: 100 µM) treatment on the growth rate characteristic and bioactive compound content variations of three spinach cultivars (Acosta-ACO; Anlani-ANL, and Matador-MTR, respectively) grown under cold conditions. While MTR was the richest variety in terms of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content, ANL was the richest variety in terms of Chl a to Chl b ratio, and xanthophyll content. Ascorbic acid and protein concentration were stimulated by MT in all three variants, but ANL had the highest value. While the proline content of cultivars decreased with only 50 MT in ACO when compared to the control, GB content decreased in MT doses. RWC increased with MT in ANL, whereas it caused to a decrease in RWC of 100 MT in ACO and 50 MT in MTR. MDA concentration was at a lower level and decreased only in ACO with 50 MT when compared to the control, while the H 2 O 2 concentration was higher at MT doses in all three species. MT treatment stimulated SOD activity in ACO and ANL and increased POD activity in all three variants. Considering the growth characteristics such as root/stem length, leaf characteristics, and biomass, exogenous MT doses had positive effects on all three cultivars. These findings suggest that foliar MT treatment can attenuate cold damage by enhancing the enzymatic and non-enzymatic antioxidant capacity of spinach seedlings, as well as the growth rate.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67126948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2102_16291654
L. Belhabib, F. Belhoucine, A. Tabeche, A. Alioua
. In the present study we investigated the concentration of organochlorine pesticides (OCPs) in the gills and muscles as well as the levels of Thiobarbituric acid-reactive substances (TBARS), Catalase, GlutathioneS-Transferase (GST) and Acetylcholinesterase (AChE) activity in the liver and muscles of the bogue Boops boops (Linneaus,1758) fish that were caught in the bay of Oran (Algeria). For the purpose of evaluating the toxicological risk in the abovementioned area. The concentrations of total Dichlorodiphényltrichloroéthane (∑DDT) was present at highly significant levels (P˃0.001) compared to other OCPs, thus displayed in the following descending order: ∑DDTs > Lindane > Heptachlor. These OCPs had largely bioaccumulated in the gills compared to muscles. The PCA projection reported significant positive correlations between the concentrations of Lindane, DichloroDiphenyldichloroEthylene (DDE), DDD, DDT, and Heptachlor in gills and muscles. Seasonal variations of (OCPs) showed the highest average concentrations in the muscle and gills of fish collected mainly in Winter and Autumn (p < 0.001). The lowest concentrations were noted during the Spring and Summer periods. Significant negative correlations existed between the CAT, TBARS, GST and Lindane, ∑DDTs, and Heptachlor. The AChE (in liver and muscles) showed significant negative correlations with total DDT, with Correlation coefficient values R = - 0.67, R = - 0.64, respectively (significant R > 0.5). This study underlines that the bay of Oran suffers from chemical defilement.
{"title":"BIOACCUMULATION AND BIOMARKER RESPONSES CAUSED BY ORGANOCHLORINE PESTICIDES IN BOGUE BOOPS BOOPS (LINNEAUS, 1758) FROM THE BAY OF ORAN, ALGERIA","authors":"L. Belhabib, F. Belhoucine, A. Tabeche, A. Alioua","doi":"10.15666/aeer/2102_16291654","DOIUrl":"https://doi.org/10.15666/aeer/2102_16291654","url":null,"abstract":". In the present study we investigated the concentration of organochlorine pesticides (OCPs) in the gills and muscles as well as the levels of Thiobarbituric acid-reactive substances (TBARS), Catalase, GlutathioneS-Transferase (GST) and Acetylcholinesterase (AChE) activity in the liver and muscles of the bogue Boops boops (Linneaus,1758) fish that were caught in the bay of Oran (Algeria). For the purpose of evaluating the toxicological risk in the abovementioned area. The concentrations of total Dichlorodiphényltrichloroéthane (∑DDT) was present at highly significant levels (P˃0.001) compared to other OCPs, thus displayed in the following descending order: ∑DDTs > Lindane > Heptachlor. These OCPs had largely bioaccumulated in the gills compared to muscles. The PCA projection reported significant positive correlations between the concentrations of Lindane, DichloroDiphenyldichloroEthylene (DDE), DDD, DDT, and Heptachlor in gills and muscles. Seasonal variations of (OCPs) showed the highest average concentrations in the muscle and gills of fish collected mainly in Winter and Autumn (p < 0.001). The lowest concentrations were noted during the Spring and Summer periods. Significant negative correlations existed between the CAT, TBARS, GST and Lindane, ∑DDTs, and Heptachlor. The AChE (in liver and muscles) showed significant negative correlations with total DDT, with Correlation coefficient values R = - 0.67, R = - 0.64, respectively (significant R > 0.5). This study underlines that the bay of Oran suffers from chemical defilement.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67127694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2102_16971710
I. Bouasla, A. Bouasla, C. Barour, S. Zekri, Y. Hafidi, T. Hamel
. The aim of the present work is the scientific evaluation of an Algerian endemic taxa ( Hypochaeris laevigata var . hipponensis Maire) widely used in folk medicine. For this objective, the active compounds, mineral element composition and antioxidant effects of three different extracts prepared from leaves were determined. Essays were started by a phytochemical screening, followed by mineral elements determination and leaf extract preparation using three organic solvents. The concentration of polyphenols, flavonoids, tannins, flavanols and orthodiphenols was determined in each extract as well as the radical scavenging activities and the total antioxidant properties. Correlation between bioactive compounds and antioxidant activities was also evaluated. Ethyl acetate extract showed the highest levels of polyphenols while the lowest one was found in raw extract, which contains the most important tannins. However, the lowest rate in anthocyanin was noted in butanol extract. Our data indicated that antioxidant properties varied according to extract type and radical scavenging used assay, indeed the half-maximal inhibitory concentration (IC 50 ) value ranged between 0.71 ± 0.02 and 7.00 ± 0.62 mg/g extract. Several positive correlations between secondary metabolite contents and antioxidant activities were registered. The obtained results support the influence of solvent extracts on bioactive compounds. These phytochemical constituents as well as mineral elements provide substantial antioxidant activities and explain the effectiveness of the studied species as traditional remedy.
{"title":"MINERAL COMPOSITION, PHYTOCHEMICAL EXPLORATION AND ANTIOXIDANT ACTIVITIES OF AN ENDEMIC TAXA: HYPOCHAERIS LAEVIGATA VAR. HIPPONENSIS MAIRE","authors":"I. Bouasla, A. Bouasla, C. Barour, S. Zekri, Y. Hafidi, T. Hamel","doi":"10.15666/aeer/2102_16971710","DOIUrl":"https://doi.org/10.15666/aeer/2102_16971710","url":null,"abstract":". The aim of the present work is the scientific evaluation of an Algerian endemic taxa ( Hypochaeris laevigata var . hipponensis Maire) widely used in folk medicine. For this objective, the active compounds, mineral element composition and antioxidant effects of three different extracts prepared from leaves were determined. Essays were started by a phytochemical screening, followed by mineral elements determination and leaf extract preparation using three organic solvents. The concentration of polyphenols, flavonoids, tannins, flavanols and orthodiphenols was determined in each extract as well as the radical scavenging activities and the total antioxidant properties. Correlation between bioactive compounds and antioxidant activities was also evaluated. Ethyl acetate extract showed the highest levels of polyphenols while the lowest one was found in raw extract, which contains the most important tannins. However, the lowest rate in anthocyanin was noted in butanol extract. Our data indicated that antioxidant properties varied according to extract type and radical scavenging used assay, indeed the half-maximal inhibitory concentration (IC 50 ) value ranged between 0.71 ± 0.02 and 7.00 ± 0.62 mg/g extract. Several positive correlations between secondary metabolite contents and antioxidant activities were registered. The obtained results support the influence of solvent extracts on bioactive compounds. These phytochemical constituents as well as mineral elements provide substantial antioxidant activities and explain the effectiveness of the studied species as traditional remedy.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67127901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2102_17891804
M. M. Alqahtani
. The biodiversity of 11 fern populations representing nine species belonging to three families ( Aspleniaceae , Adiantaceae , and Pteridaceae ) was evaluated using morphological variations and molecular polymorphism generated by 12 ISSR primers. Substantial variations were scored in 16 morphological characters and reflected in the cluster analysis of the examined accessions based on morphological variation. In the phylogenetic relationships generated based on ISSR polymorphism and morphological variations, the three Cheilanthes species clustered with Asplenium adiantum nigrum and were delimited from other species, which supports that A. adiantum nigrum may be related to Cheilanthes species. In the meantime, the two populations of Asplenium ceterach and the two populations of Asplenium aethiopicum were differentiated as one cluster. These results are congruent with the cluster based on the analysis of ISSR data except for the association of Adiantum capillus veneris and Asplenium trichomanes , which revealed that A. trichomanes may be related to Adiantum capillus veneris . Also, these results were confirmed by the tree obtained based on the Elucedine coefficient and with the population grouping based on principal component analysis (PCA) using the PAST program. Also, Online Marker Efficiency Calculator (iMEC) supports the use of ISSR markers for examining the genetic diversity across fern genotypes.
{"title":"BIODIVERSITY OF SOME PTERIDOPHYTES SPECIES AND THEIR MORPHOLOGICAL CHARACTERISTICS FROM THE SOUTHWEST OF SAUDI ARABIA","authors":"M. M. Alqahtani","doi":"10.15666/aeer/2102_17891804","DOIUrl":"https://doi.org/10.15666/aeer/2102_17891804","url":null,"abstract":". The biodiversity of 11 fern populations representing nine species belonging to three families ( Aspleniaceae , Adiantaceae , and Pteridaceae ) was evaluated using morphological variations and molecular polymorphism generated by 12 ISSR primers. Substantial variations were scored in 16 morphological characters and reflected in the cluster analysis of the examined accessions based on morphological variation. In the phylogenetic relationships generated based on ISSR polymorphism and morphological variations, the three Cheilanthes species clustered with Asplenium adiantum nigrum and were delimited from other species, which supports that A. adiantum nigrum may be related to Cheilanthes species. In the meantime, the two populations of Asplenium ceterach and the two populations of Asplenium aethiopicum were differentiated as one cluster. These results are congruent with the cluster based on the analysis of ISSR data except for the association of Adiantum capillus veneris and Asplenium trichomanes , which revealed that A. trichomanes may be related to Adiantum capillus veneris . Also, these results were confirmed by the tree obtained based on the Elucedine coefficient and with the population grouping based on principal component analysis (PCA) using the PAST program. Also, Online Marker Efficiency Calculator (iMEC) supports the use of ISSR markers for examining the genetic diversity across fern genotypes.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67128049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2102_18231835
S. Jaafar, M. Suhaimi, N. A. Maznan, C. Safuan, C. Tan, M. Yucharoen, J. Saidin, Z. Bachok
. Coral reefs in Southeast Asia are becoming progressively degraded due to the natural and anthropogenic disturbances which lead to poor water quality threatening the reefs’ health. Coral disease is one of the threats to the coral reefs worldwide but research on coral disease in Southeast Asia is relatively scarce especially on bacterial community associated with the disease. The destructive coral black band disease (BBD) can be found widely in the southern region of the South China Sea, Malaysia. This study aims to compare the microbial communities of healthy and BBD-infected coral tissue from two different coral species Montipora turtlensis and Acropora spicifera collected at the Shark Point of Pulau Redang, Terengganu. The bacterial isolated from corals nubbins were examined by combining culture-dependent method and bacterial sequencing of 16S rDNA. A total of 18 bacteria isolates were identified from both species of coral tissue samples prior to sub-culturing colonies samples. The sequences detected were derived from a wide taxonomic range, including representatives of Actinobacteria, Furmicutes and Proteobacteria phyla. Bacteria belonging to the phyla Proteobacteria were further divided into alpha and gammaproteobacteria. Most of the bacteria identified can be found on healthy and BBD-affected coral tissue samples; however, there are few bacteria which can be only found on BBD-affected tissue of respective coral species such as Lelliottia sp., Salnicola salarius and Microbacterium petrolearium for M. turtlensis while Pseudomonas stutzeri and Erythrobacter sp. for A. spicifera.
{"title":"ISOLATION AND IDENTIFICATION OF BACTERIAL COMMUNITIES FROM CORAL TISSUE AFFECTED BY BLACK BAND DISEASE AT PULAU REDANG, TERENGGANU, MALAYSIA","authors":"S. Jaafar, M. Suhaimi, N. A. Maznan, C. Safuan, C. Tan, M. Yucharoen, J. Saidin, Z. Bachok","doi":"10.15666/aeer/2102_18231835","DOIUrl":"https://doi.org/10.15666/aeer/2102_18231835","url":null,"abstract":". Coral reefs in Southeast Asia are becoming progressively degraded due to the natural and anthropogenic disturbances which lead to poor water quality threatening the reefs’ health. Coral disease is one of the threats to the coral reefs worldwide but research on coral disease in Southeast Asia is relatively scarce especially on bacterial community associated with the disease. The destructive coral black band disease (BBD) can be found widely in the southern region of the South China Sea, Malaysia. This study aims to compare the microbial communities of healthy and BBD-infected coral tissue from two different coral species Montipora turtlensis and Acropora spicifera collected at the Shark Point of Pulau Redang, Terengganu. The bacterial isolated from corals nubbins were examined by combining culture-dependent method and bacterial sequencing of 16S rDNA. A total of 18 bacteria isolates were identified from both species of coral tissue samples prior to sub-culturing colonies samples. The sequences detected were derived from a wide taxonomic range, including representatives of Actinobacteria, Furmicutes and Proteobacteria phyla. Bacteria belonging to the phyla Proteobacteria were further divided into alpha and gammaproteobacteria. Most of the bacteria identified can be found on healthy and BBD-affected coral tissue samples; however, there are few bacteria which can be only found on BBD-affected tissue of respective coral species such as Lelliottia sp., Salnicola salarius and Microbacterium petrolearium for M. turtlensis while Pseudomonas stutzeri and Erythrobacter sp. for A. spicifera.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67128335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15666/aeer/2103_18551867
K. Anjali, A. Balasubramanian, S. Radhakrishnan, K. Ramah, P. Boominathan, C.N. HARI PRASATH
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