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Development and Temperature-induced Expression of a HSP90 Gene Lacking Introns in Cotesia chilonis (Hymenoptera: Braconidae) 一个缺乏内含子的HSP90基因在辣椒茧蜂中的发育和温度诱导表达(膜翅目:茧蜂科)
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-09-03 DOI: 10.1093/aesa/saac015
Shuan-Gan Li, Chuanlei Dong, Feng Zhu, Fei Yang, M. Lu, Yu-Zhou Du
Abstract Cotesia chilonis (Munakata) is the predominant parasitic wasp of Chilo suppressalis Walker (Lepidoptera: Pyralidea), a major rice pest in China. As climate change continues to intensify, C. chilonis and C. suppressalis must adapt to the increased frequency of adverse temperatures. In this study, the novel Cchsp90-2 was isolated and characterized from C. chilonis. The full-length Cchsp90-2 cDNA was 2689 bp and encoded 728 amino acids with an isoelectric point of 4.93. Cchsp90-2 encodes three heat shock protein 90 (HSP90) family signatures, and the alignment of genomic and cDNA sequences revealed that Cchsp90-2 does not contain introns. Real-time quantitative PCR revealed that Cchsp90-2 was induced in eggs and adults; however, larvae and pupae showed no significant differences in Cchsp90-2 expression levels. Cchsp90-2 was induced at both high and low temperatures, with very high expression levels after a 2-h exposure to 33°C or –13°C. This study provides insight into the mechanistic basis of thermal tolerance in C. chilonis and has implications for deploying this wasp to control C. suppressalis.
摘要Chilonia(Munakata)是我国水稻主要害虫二化螟(鳞翅目:梨目)的主要寄生蜂。随着气候变化的持续加剧,辣椒和二化螟必须适应日益频繁的不利温度。本研究从C.chilonis中分离并鉴定了新的Cchhsp90-2。Cchhsp90-2 cDNA全长2689bp,编码728个氨基酸,等电点为4.93。Ccsp90-2编码三个热休克蛋白90(HSP90)家族特征,基因组和cDNA序列比对表明Ccsp90-2不含内含子。实时定量PCR显示Cchhsp90-2在卵和成虫中均有诱导作用;幼虫和蛹Cchhsp90-2的表达水平没有显著差异。Cchhsp90-2在高温和低温下都被诱导,在33°C或-13°C下暴露2小时后具有非常高的表达水平。这项研究深入了解了chilonis耐热性的机制基础,并对部署这种黄蜂来控制二化螟具有启示意义。
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引用次数: 0
Nitrogen Inventories and Nitrogenase Expression Rates of a Drywood and a Subterranean Termite 枯木和地下白蚁的氮含量和固氮酶表达率
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-09-01 DOI: 10.1093/aesa/saac014
A. Mullins, R. Scheffrahn, N. Su
Abstract Recent research has shown that a subterranean termite (Coptotermes formosanus Shiraki) is dependent on dietary nitrogen in order to fulfill it's nitrogen requirements for colony growth and development. Crypototermes brevis (Walker) was chosen as a model for comparison, as it has no access to dietary nitrogen outside of it's wood diet. A full nitrogen inventory before and after colony growth was conducted in order to determine the species' dependence on atmospheric N2 fixation. In addition, an expression assay was conducted for a gene encoding a nitrogenase subunit (nifH). Nitrogenase expression was then compared between C. formosanus and Cr. brevis. Results showed that N2 fixation was an important source of nitrogen acquisition for Cr. brevis, and nitrogenase expression was approximately four-fold higher in Cr. brevis than in C. formosanus. The nitrogenase assay used in this study is proposed as a potential tool to measure dependence on N2 fixation versus dietary nitrogen roles in diverse species of termite.
摘要近年来的研究表明,地下白蚁(Coptotermes formosanus Shiraki)依赖于饲料中的氮来满足其群体生长和发育对氮的需求。选择短白蚁作为比较模型,因为它在木材饮食之外无法获得膳食氮。为了确定该物种对大气固氮的依赖,在菌落生长前后进行了全氮清查。此外,对编码氮酶亚基(nifH)的基因进行了表达测定。然后比较了台湾镰刀菌和短链镰刀菌之间的氮酶表达。结果表明,氮固定是短链镰刀菌获得氮的重要来源,短链镰刀菌的氮酶表达量约为台湾镰刀菌的4倍。本研究中使用的氮酶测定被认为是一种潜在的工具,可以衡量不同种类白蚁对N2固定的依赖程度和饮食中氮的作用。
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引用次数: 1
Insect Floral Visitors of Ptelea trifoliata (Rutaceae) in Iowa, United States. 美国爱荷华州三叶草(芸香科)的昆虫访花者。
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-07-08 eCollection Date: 2022-09-01 DOI: 10.1093/aesa/saac012
A J Talcott Stewart, M E O'Neal, W R Graves

Ptelea trifoliata L., is a North American tree that supports insect communities through floral rewards. Our objectives were to determine the importance of insects as pollinators of P. trifoliata; describe the community of floral visiting insects of P. trifoliata in Iowa, where no such information was available; and to note insect preferences for male or female flowers. Over two years, inflorescences on 13 trees were covered with mesh bags before blooming and the amount of fruit produced was compared to uncovered inflorescences from the same trees. In one year, insects were collected from male and female trees with an insect vacuum every 3 h between 7 am and 7 pm from four sites in Iowa, USA between 30 May and 16 June 2020. In 2019 and 2020, almost no fruit set occurred from inflorescences covered with mesh bags while an average of 51.2 fruits formed on unbagged inflorescences (P < 0.0001), suggesting insects larger than the 600 μm pore diameters mesh were responsible for pollination of P. trifoliata. Insects from five orders, 49 families, and at least 109 species were collected. Most insects were Hymentoptera (48.3%) or Diptera (28.2%). Male flowers attracted 62.3% of all insects collected. Since most of the insects found visiting P. trifoliata were not bees, the floral rewards of the flowers may be a valuable resource for a wide variety of insects in the central United States.

Ptelea trifoliata L.,是一种北美树木,通过花的奖励来支持昆虫群落。我们的目的是确定昆虫作为三叶草传粉者的重要性;描述了没有相关资料的爱荷华州三叶甘油酯花访虫的群落;并注意昆虫对雄花和雌花的偏好。在两年多的时间里,研究人员在开花前用网袋覆盖了13棵树的花序,并将其果实数量与同一棵树未覆盖的花序进行了比较。在一年内,在2020年5月30日至6月16日期间,在美国爱荷华州的四个地点,在早上7点至晚上7点之间,每隔3小时用昆虫真空吸尘器从雄性和雌性树木上收集昆虫。在2019年和2020年,覆盖网袋的花序几乎不结实,而未覆盖网袋的花序平均结实51.2个(P < 0.0001),表明孔径大于600 μm网袋的昆虫负责授粉。共收集昆虫5目49科至少109种。昆虫以膜翅目(48.3%)和双翅目(28.2%)居多。雄花吸引了62.3%的昆虫。由于造访三叶草的大多数昆虫都不是蜜蜂,因此在美国中部,三叶草的花朵回报可能是多种昆虫的宝贵资源。
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引用次数: 0
The First Biological Portrait of Stalk-Eyed Fruit Flies: Life History, Reproductive Biology and Host Use Patterns in Pelmatops spp. (Diptera:Tephritidae) 茎眼果蝇的第一幅生物图谱:Pelmatops spp.的生活史、繁殖生物学和寄主利用模式(直翅目:地蝇科)
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-07-04 DOI: 10.1093/aesa/saac011
Ning Huangfu, Yong Wang, Yu Zeng, T. Chua, C. Zhu, Xiaolin Chen
Abstract The stalk-eyed fruit flies, with their eyes borne at the ends of long stalks, are distinctly different from all other members of the family Tephritidae (Diptera). They resemble stalk-eyed flies (Diptera, Diopsidae) but they are much larger and their antennae are located in the middle of the head instead of on the eye stalks. The stalk-eyed fruit flies are represented by two genera (Pelmatops Enderlein and Pseudopelmatops Shiraki) mainly found in the Oriental tropics and subtropics, but their basic biology remains poorly documented. Here, we describe the life history, reproductive biology, and host use patterns of Pelmatops spp. (mainly P. ichneumoneus (Westwood)). These flies used two local brambles, Rubus setchuenensis and R. multibracteatus (Rosales, Rosaceae), as hosts, with females laying eggs below the epidermal tissue.The larvae bore into the stem, where they feed, eventually dropping to the ground to pupate in the soil. We describe the pupal morphology and eclosion, including the elongation of their eye stalks, feeding, mating, and agonistic behavior in adults. We observed mating between female P. ichneumoneus and male P. tangliangi and tentatively suggest that the two species could be conspecific. Our work presents the first detailed report on the biology of stalk-eyed fruit flies and it lays a significant foundation for future studies on the ecology and evolution of this group.
摘要茎眼果蝇的眼睛长在茎的末端,与地蝇科的所有其他成员有明显的不同。它们类似于眼蝇(直翅目,直翅目),但它们要大得多,它们的触角位于头部中部,而不是眼柄上。柄眼果蝇有两个属(Pelmatops Enderlein和Pseudopelmatops Shiraki),主要分布在东方热带和亚热带,但它们的基本生物学记录很少。在这里,我们描述了Pelmatops spp.(主要是P.ichneumoneus(Westwood))的生活史、生殖生物学和宿主使用模式。这些苍蝇使用两种当地的荆棘,悬钩子(Rubus setchuenensis)和R.multibracteatus(Rosales,Rosaceae)作为宿主,雌性在表皮组织下产卵。幼虫钻入茎中,在那里觅食,最终落到地上,在土壤中化蛹。我们描述了蛹的形态和羽化,包括它们眼柄的伸长、进食、交配和成虫的痛苦行为。我们观察到雌性伊氏肺吸虫和雄性唐氏肺吸虫之间的交配,并初步认为这两个物种可能是同种的。我们的工作首次详细报道了茎眼果蝇的生物学,为未来研究该类群的生态学和进化奠定了重要基础。
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引用次数: 2
Role of stereotactic body radiation in the enhancement of the quality of life in locally advanced pancreatic adenocarcinoma: a systematic review. 立体定向体放射在提高局部晚期胰腺癌患者生活质量中的作用:系统综述。
IF 3.6 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-06-17 DOI: 10.1186/s13014-022-02076-5
Marlies Vornhülz, Sofia Anton, Balint Eross, Zsolt Szakács, Peter Hegyi, Ivonne Regel, Claus Belka, Maximilian Niyazi, Julia Mayerle, Georg Beyer

Introduction: Up to 30% of pancreatic cancer patients initially present locally advanced (LAPC). Stereotactic body radiation therapy (SBRT) may be an additional palliative treatment option when curative resection is no longer achievable. Our systematic review aimed to assess the effect of SBRT on the quality of life in LAPC.

Methods: We searched five databases until June 29th, 2021, for original articles that reported on SBRT for histologically proven LAPC in adults. Data were extracted on study characteristics, SBRT and additional therapy regimen, pain, biliary complications, nutrition, quality of life and other patient-reported outcomes. Statistical analyses were performed for population and survival data.

Results: 11 case series studies comprising 292 patients with a median age of 66 (range 34-89) years were included in the final analysis. The weighted average BED2;10 (radiation biologically effective dose, equivalent dose in 2 Gy fractions) was 54 Gy, delivered in 3 to 6 fractions. The individual studies used different scales and endpoints, not allowing a meta-analysis. Pain generally appeared to be improved by SBRT. SBRT significantly reduced jaundice. Local control was achieved in 71.7% of patients. Weight loss and nausea also tended to improve after SBRT.

Conclusion: SBRT of locally advanced irresectable pancreatic cancer is a promising approach for achieving local control and improving the quality of life. However, randomized controlled trials with larger cohorts are needed to assess the value of SBRT in pancreatic cancer therapy.

简介多达 30% 的胰腺癌患者最初为局部晚期(LAPC)。当无法进行根治性切除时,立体定向体放射治疗(SBRT)可能是一种额外的姑息治疗选择。我们的系统综述旨在评估 SBRT 对 LAPC 患者生活质量的影响:截至 2021 年 6 月 29 日,我们在五个数据库中检索了报告 SBRT 治疗经组织学证实的成人 LAPC 的原始文章。我们提取了有关研究特征、SBRT 和附加治疗方案、疼痛、胆道并发症、营养、生活质量和其他患者报告结果的数据。对人口和生存数据进行了统计分析:最终分析包括 11 项病例系列研究,共 292 名患者,中位年龄为 66 岁(34-89 岁)。加权平均 BED2;10(辐射生物有效剂量,以 2 Gy 为单位的等效剂量)为 54 Gy,分 3 至 6 次给药。各项研究使用了不同的量表和终点,因此无法进行荟萃分析。SBRT似乎普遍改善了疼痛。SBRT能明显减轻黄疸。71.7%的患者达到了局部控制。SBRT治疗后,体重减轻和恶心症状也趋于改善:SBRT治疗局部晚期不可切除的胰腺癌是一种很有前景的方法,可实现局部控制并改善生活质量。然而,要评估SBRT在胰腺癌治疗中的价值,还需要进行更大规模的随机对照试验。
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引用次数: 7
Reviewers for Annals of the Entomological Society of America(November 2020–October 2021) 美国昆虫学会年鉴审稿人(2020年11月- 2021年10月)
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-05-25 DOI: 10.1093/aesa/saac003
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引用次数: 0
Four Insulin-Like Peptides Orchestrate Reproductive Signaling of the Green Lacewing, Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae) 四种胰岛素样肽调控绿草蛉的生殖信号(神经翅目:草蛉科)
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-05-12 DOI: 10.1093/aesa/saac007
Tingting Zhang, Xiaopin Liu, Lisheng Zhang, Mengqing Wang, Yuyan Li, J. Mao
Abstract In insects, insulin signaling plays a pivotal role in the regulation of growth, metabolism, reproduction, and aging. Revealing the regulatory roles of insulin signaling in natural enemy insects is helpful for developing artificial approaches to promote reproduction. In this study, the expression of four insulin-like peptides (ILPs) in female adults of the natural predator Chrysopa pallens was analyzed by quantitative real-time-polymerase chain reaction (qRT-PCR) and their functions were investigated by RNA interference (RNAi). C. pallens ILP1 (CpILP1) and C. pallens ILP4 (CpILP4) showed a gradual increase in mRNA levels from early adult stages to later stages. However, C. pallens ILP2 (CpILP2) and C. pallens ILP3 (CpILP3) had an opposite expression curve with a high transcript level at the beginning of the adult stage and a gradual reduction thereafter. All four CpILPs showed high expression levels in the ovary, but CpILP2 transcripts were also abundant in fat bodies. Disruption of CpILP1 and CpILP2 expression by RNAi-mediated knockdown strikingly suppressed ovarian development and reduced C. pallens vitellogenin gene 1 (CpVg1) transcripts. Depletion of CpILP3 transcripts did not significantly decrease ovariole number but drastically reduced CpVg1 expression. Interference with the expression of CpILP4 significantly inhibited ovarian development but did not suppress CpVg1 expression. All gene knockdowns failed to dramatically impair total fecundity and egg quality.These data suggest that the four C. pallens ILPs are involved in reproductive signaling at different degrees.
在昆虫中,胰岛素信号在生长、代谢、繁殖和衰老的调节中起着关键作用。揭示胰岛素信号在天敌昆虫中的调控作用,有助于开发人工促进昆虫繁殖的方法。本研究采用定量实时聚合酶链反应(qRT-PCR)技术分析了天然捕食者黄斑蝶雌性成虫体内4种胰岛素样肽(ILPs)的表达,并采用RNA干扰(RNAi)技术研究了它们的功能。C. pallens ILP1 (CpILP1)和C. pallens ILP4 (CpILP4) mRNA水平从成虫早期到后期逐渐升高。而淡紫色C. pallens ILP2 (CpILP2)和淡紫色C. pallens ILP3 (CpILP3)的表达曲线相反,在成虫期开始时转录量较高,之后逐渐降低。这四种CpILP2在卵巢中均有高表达,但CpILP2转录本在脂肪体中也大量表达。通过rnai介导的敲低破坏CpILP1和CpILP2的表达,显著抑制卵巢发育,并降低淡黄色葡萄球菌卵黄原蛋白基因1 (CpVg1)转录物。CpILP3转录本的缺失没有显著减少卵巢数量,但显著降低CpVg1的表达。干扰CpILP4的表达可显著抑制卵巢发育,但不抑制CpVg1的表达。所有的基因敲低都没有显著地降低总繁殖力和卵子质量。这些数据表明,四种黄颡鱼ILPs在不同程度上参与生殖信号传导。
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引用次数: 1
Prospective, multicenter study of 2-level cervical arthroplasty with a PEEK-on-ceramic artificial disc. 使用陶瓷基 PEEK 人工椎间盘进行 2 级颈椎关节置换术的前瞻性多中心研究。
IF 2.8 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-04-01 Print Date: 2022-09-01 DOI: 10.3171/2022.1.SPINE211264
Domagoj Coric, Richard D Guyer, Hyun Bae, Pierce D Nunley, K Brandon Strenge, John H Peloza, Margaret O Boltes, Donna D Ohnmeiss

Objective: The purpose of this study was to evaluate the safety and efficacy of a PEEK-on-ceramic cervical total disc replacement (cTDR) device for the treatment of 2-level cervical disc disease with radiculopathy and/or myelopathy.

Methods: The study was a prospective, nonrandomized, historically controlled FDA investigational device exemption trial evaluating the Simplify Cervical Artificial Disc for use at 2 levels. The anterior cervical discectomy and fusion (ACDF) control group was derived from a propensity score-matched (using subclassification) cohort of patients who participated in an earlier prospective trial in which similar indications were used. The follow-up duration was 24 months. The primary outcome was a 4-point composite success classification. Other validated clinical and radiographic assessments were also evaluated.

Results: The investigational group (n = 182) was compared with patients who underwent ACDF (n = 170) in a historical control group using propensity score analysis. The overall composite success rate was statistically significantly greater in the cTDR group compared with the ACDF group (86.7% vs 77.1%; p < 0.05). The mean Neck Disability Index scores improved significantly in both groups, with cTDR significantly lower at some follow-up points. At the 24-month follow-up, a minimum 15-point improvement in Neck Disability Index scores was achieved in 92.9% of the cTDR group and 83.5% of the ACDF group (p > 0.05). In both groups, neck and arm pain scores improved significantly (p < 0.05) by 6 weeks and improvement was maintained throughout follow-up. Segmental range of motion was maintained at both treated segments in the cTDR group. MRI performed in the cTDR group at 24 months postoperatively found minimal changes in facet joint degeneration. The rate of subsequent surgical intervention was 2.2% in the cTDR group and 8.8% in the ACDF group.

Conclusions: This study adds to the growing body of literature supporting cTDR for 2-level cervical disc disease with radiculopathy or myelopathy. cTDR showed a superior overall success rate compared to ACDF, while maintaining motion. These results support that the Simplify disc is a viable alternative to ACDF in appropriately selected patients with 2-level cervical spondylosis.

研究目的本研究旨在评估陶瓷基 PEEK 颈椎全椎间盘置换(cTDR)装置治疗伴有根性病变和/或脊髓病的 2 级颈椎椎间盘疾病的安全性和有效性:该研究是一项前瞻性、非随机、历史对照的 FDA 研究设备豁免试验,对用于 2 个水平的 Simplify 颈椎人工椎间盘进行评估。前路颈椎椎间盘切除和融合术(ACDF)对照组来自于一个倾向得分匹配(使用亚分类)的患者队列,这些患者参加了早期的前瞻性试验,在该试验中使用了类似的适应症。随访时间为 24 个月。主要结果为 4 点复合成功率分类。此外,还对其他经过验证的临床和放射学评估结果进行了评估:研究组(n = 182)与历史对照组中接受 ACDF 的患者(n = 170)进行了比较,采用倾向得分分析法。与 ACDF 组相比,cTDR 组的总体综合成功率明显更高(86.7% vs 77.1%;P < 0.05)。两组患者的颈部残疾指数平均得分均有明显改善,而 cTDR 在某些随访点的得分明显更低。在 24 个月的随访中,92.9% 的 cTDR 组和 83.5% 的 ACDF 组的颈部残疾指数评分至少提高了 15 分(p > 0.05)。两组患者的颈部和手臂疼痛评分在 6 周前均有明显改善(p < 0.05),并在整个随访期间保持改善。在 cTDR 组中,两个治疗节段的活动范围均得以保持。cTDR 组术后 24 个月时进行的核磁共振成像发现,面关节退行性变的变化极小。cTDR 组的后续手术干预率为 2.2%,ACDF 组为 8.8%:这项研究为越来越多支持 cTDR 治疗伴有根性病变或脊髓病的 2 级颈椎间盘疾病的文献增添了新的内容。与 ACDF 相比,cTDR 显示出更高的总体成功率,同时还能保持运动。这些结果证明,对于经过适当选择的 2 级颈椎病患者,Simplify 椎间盘是 ACDF 的可行替代方案。
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引用次数: 0
Diversity and Climatic Distribution of Moths in the Tribe Arctiini (Lepidoptera: Erebidae: Arctiinae) in Mexico 墨西哥飞蛾族飞蛾的多样性和气候分布(鳞翅目:飞蛾科:飞蛾科)
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-02-21 DOI: 10.1093/aesa/saac002
M. Montañez-Reyna, J. León-Cortés, F. Infante, E. Naranjo, A. Gómez-Velasco
Abstract The Mexican lepidopteran fauna is particularly diverse, but many moth groups remain poorly documented. The tribe Arctiini is a species-rich group that has been used as a reliable indicator of environmental change. However, little is known about the fauna of the tribe Arctiini in Mexico, and there is no exhaustive review of its diversity and distribution patterns. Our aims were: 1) to account for the species diversity and distribution patterns of the tribe Arctiini; 2) to build spatial distributions and discuss possible changes in the distribution areas of the tribe Arctiini using conservative (RCP 2.6) and liberal (RCP 8.5) future climate scenarios; and 3) to discuss the conservation implications for key taxa that due to their life history characteristics and restricted distribution, might require particular conservation actions. We compiled a total of 16,385 records and 548 species in seven subtribes. Diversity profiles revealed higher cumulative species richness and diversity for the subtribes Phaegopterina, Ctenuchina, and Euchromiina, and we identified a pattern of decreasing species diversity with elevation. In addition, we estimated that 35% and 84% of modeled species in future conservative and liberal climatic scenarios, respectively, would result in significant losses of climatic suitability and shifts in spatial distribution. The endemic species, Virbia semirosea, Poliopastea jalapensis, and Pygoctenucha azteca would likely reduce their distribution by approximately 50% in both climatic scenarios. Maintaining a network of highly threatened habitats (e.g., cloud forests, tropical rain forests) will be essential to preserve persisting species populations and to increase likely (re)colonization events.
墨西哥鳞翅目动物群特别多样化,但许多蛾类仍然缺乏文献记录。Arctiini部落是一个物种丰富的群体,被用作环境变化的可靠指标。然而,人们对墨西哥artiini部落的动物群知之甚少,也没有对其多样性和分布模式进行详尽的审查。我们的目标是:1)解释Arctiini部落的物种多样性和分布模式;2)在保守(RCP 2.6)和自由(RCP 8.5)两种气候情景下,建立大山竹群落的空间分布格局,探讨其分布区域可能发生的变化;3)讨论了由于生活史特征和分布限制,可能需要采取特殊保护措施的关键类群的保护意义。共收集到7个亚族的16385条记录和548种。Phaegopterina亚族、Ctenuchina亚族和Euchromiina亚族的累积物种丰富度和多样性较高,且物种多样性呈随海拔升高而降低的趋势。此外,我们估计在未来的保守和自由气候情景下,分别有35%和84%的模拟物种将导致气候适宜性的显著丧失和空间分布的变化。在这两种气候情景下,特有物种,半玫瑰Virbia, jalapensis和Pygoctenucha azteca可能会使其分布减少约50%。维持一个高度受威胁的栖息地网络(如云雾林、热带雨林)对于保护持续存在的物种种群和增加可能的(再)殖民事件至关重要。
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引用次数: 3
Changes in Functional and Numerical Responses of the Parasitoid Wasp Trichogramma brassicae (Hymenoptera: Trichogrammatidae) Over 45 Generations of Rearing on Ephestia kuehniella 芸苔赤眼蜂(膜翅目:赤眼蜂科)饲养45代后功能及数值响应的变化
IF 2.3 3区 农林科学 Q1 ENTOMOLOGY Pub Date : 2022-02-15 DOI: 10.1093/aesa/saac004
Ehsan Ghaemmaghami, Y. Fathipour, A. Bagheri, Ali Asghar Talebi, G. Reddy
Abstract We assessed the functional and numerical responses of Trichogramma brassicae Bezdenko under long-term rearing (45 generations, G5-G45) on eggs of a common factitious host, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Under such long-term mass rearing, the function response of this parasitoid shifted from Type III (from G5 to G20) toType II (from G25 to G45).The maximum attack rate (T/Th) (33.62 hosts/day) and the shortest handling time (0.7138 ± 0.0272 h) were recorded in G5. Also, G45 had the lowest attack rate (21.67 hosts/day) and longest handling time (1.1076 ± 0.0689 h). The handling time increased gradually over the generations (P reg <0.01, R2 = 0.903). The number of eggs laid by the parasitoid increased significantly with increased host density, but reached a plateau at very high densities. While no significant differences in daily parasitism were observed over 45 generations at densities of 2, 4, 8, and 16 available host eggs per wasp, there were significant differences in parasitism at host densities of 32, 64, and 128 eggs per wasp. T. brassicae females were more active and foraged more frequently in earlier generations (G5 to G20), which allowed wasps to parasitize more E. kuehniella eggs compared to later generations. Also, continuous mass rearing of T. brassicae negatively affected foraging behavior and the quality of wasps after G20. However, rejuvenation of the colony by adding field-collected parasitoids or rearing wasps on more suitable factitious hosts is strongly recommended to lower the negative effects of long-term mass rearing on quality of this parasitoid.
摘要研究了长期饲养(45代,G5-G45)的芸赤眼蜂(Trichogramma brassicae Bezdenko)对kuehniella Zeller(鳞翅目:Pyralidae)寄主卵的功能和数值反应。在这种长期的大量饲养下,该寄生蜂的功能响应从III型(从G5到G20)转变为II型(从G25到G45)。G5的攻击速率最大(T/Th)为33.62台/天,处理时间最短(0.7138±0.0272 h)。G45的攻击率最低(21.67台/d),处理时间最长(1.1076±0.0689 h),处理时间随世代逐渐增加(P <0.01, R2 = 0.903)。随着寄主密度的增加,产卵量显著增加,但在高密度时达到平稳期。在每只寄生蜂2、4、8和16个有效寄主卵密度下,45代寄生蜂的日寄生率差异不显著,而在每只寄生蜂32、64和128个有效寄主卵密度下,寄生蜂的日寄生率差异显著。早代(G5 ~ 20代)的芸苔科雌蜂更活跃,觅食频率也更高,这使得与后代相比,芸苔科雌蜂能够寄生更多的库氏伊蚊卵。二十国集团后,持续大量饲养芸苔蚧对胡蜂的觅食行为和品质产生负面影响。但是,强烈建议通过添加田间采集的寄生蜂或在更合适的人工寄主上饲养寄生蜂来恢复种群,以降低长期大量饲养对寄生蜂质量的负面影响。
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引用次数: 2
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Annals of The Entomological Society of America
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