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Morphological and Molecular Characterization of Fusarium chlamydosporum, F. brachygibbosum and F. flocciferum Associated with Crown and Root Rot of Wheat 与小麦冠腐病和根腐病相关的衣孢镰刀菌、短毛镰刀菌和絮状镰刀菌的形态和分子特征
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-040.2.175181
L. Zidan, D. Jawdat, W. Naffaa
Zidan, L., D. Jawdat and W. Naffaa. 2022. Morphological and Molecular Characterization of Fusarium chlamydosporum, F. brachygibbosum and F. flocciferum Associated with Crown and Root Rot of Wheat. Arab Journal of Plant Protection, 40(2): 175-181. https://doi.org/10.22268/AJPP-040.2.175181 Fusarium is one of the most important genera of fungi, causing plant, animal, and human diseases and produces mycotoxins. A total of 105 isolates of Fusarium spp. were recovered from crowns and roots of wheat plants, showing typical symptoms, collected from four Syrian provinces during 2017-2018. In previous studies, seventeen Fusarium species associated with crown and root rot (FCR) on wheat were identified in Syria. However, the identity of some isolates has not been resolved. The current study was carried out to identify and characterize through morphological approaches and sequencing a partial translation elongation factor 1-alpha (TEF1-α) gene in three Fusarium species F. chlamydosporum Wollenweber & Reinking, F. brachygibbosum Padwick and F. flocciferum Corda associated with FCR, isolated and identified for the first time in Syria. The present study will provide detailed cultural and morphological characteristics of the three species, scarcely described in literature. Keywords: First record, morphological descriptions, TEF1-α gene, Syria.
Zidan, L., D. Jawdat和W. Naffaa. 2022。与小麦冠腐病和根腐病相关的衣孢镰刀菌、短毛镰刀菌和絮状镰刀菌的形态和分子特征。植物保护学报,40(2):175-181。https://doi.org/10.22268/AJPP-040.2.175181镰刀菌是最重要的真菌属之一,引起植物、动物和人类疾病并产生真菌毒素。2017-2018年,从叙利亚4省小麦植株的冠和根中分离出镰刀菌105株,表现出典型症状。在以往的研究中,在叙利亚鉴定出17种与小麦冠根腐病(FCR)有关的镰刀菌。然而,一些分离株的身份尚未得到解决。目前的研究是通过形态学方法和测序来鉴定和表征与FCR相关的三种镰孢菌F. chlamydosporum Wollenweber & Reinking, F. brachygibbosum Padwick和F. ciferum Corda的部分翻译延伸因子1-α (TEF1-α)基因,这三种镰孢菌在叙利亚首次分离和鉴定。本研究将提供详细的文化和形态特征的三个物种,很少在文献中描述。关键词:首次记录,形态描述,TEF1-α基因,叙利亚
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引用次数: 0
Survey for Legume and Cereal Viruses in Libya 利比亚豆科和谷类病毒调查
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-40.3.222230
Hatem Abukraa, S. Kumari, Fawzi Bshia
Abukraa, H., S.G. Kumari and F. Bshia. 2022. Survey for Legume and Cereal Viruses in Libya. Arab Journal of Plant Protection, 40(3): 222-230. https://doi.org/10.22268/AJPP-40.3.222230 A field survey was conducted during March and April 2010, to identify viral diseases affecting cereal and legume crops in different regions of Libya. A total of 3706 barley and wheat samples were collected randomly in addition to 187 symptomatic samples from 22 barley and 20 wheat fields. Moreover, 34 symptomatic legume samples were collected from two faba bean fields and one field each of of lentil, chickpea, Vicia sativa, Vicia ervillia and Vicia narbonesis. All samples were tested at ICARDA Virology Laboratory by tissue blot immunoassay (TBIA) using specific antibodies. Results of random cereal samples showed that Barley yellow dwarf virus-PAV (BYDVPAV) was more common (12.6%) in wheat and barley samples, followed by Wheat dwarf virus (WDV) (1.1%). In symptomatic samples, BYDV-PAV was also the most common (45 out of 187 samples tested were infected: 45/187), followed by WDV (23/187), Barley yellow striate mosaic virus (BYSMV) (40/187) and Maize streak virus (MSV) (7/187). In legume fields, Soybean dwarf virus (SbDV) 13 out of 34 samples tested were infected (13/34) was the most common, followed by Bean leafroll virus (BLRV) (12/34), Chickpea chlorotic stunt virus (CpCSV) (5/34) and Beet western yellows virus (BWYV) (4/34). Serological results were confirmed by amplification with polymerase chain reaction (PCR) using specific primers. This is the first report of WDV, MSV and BYSMV on cereal crops, and BLRV, BWYV, SbDV and CpCSV on legume crops in Libya. Keywords: Libya, Cereals, Legumes, Viruses, TBIA, PCR
Abukraa, H., S.G. Kumari和F. Bshia., 2022。利比亚豆科和谷类病毒调查。植物保护学报,40(3):222-230。https://doi.org/10.22268/AJPP-40.3.222230 2010年3月和4月期间进行了一次实地调查,以查明影响利比亚不同地区谷物和豆类作物的病毒性疾病。随机抽取大麦和小麦样品3706份,22块大麦和20块小麦样品187份。另外,从2块蚕豆田和1块扁豆、鹰嘴豆、豇豆、豇豆和豇豆田中采集了34份有症状的豆类样品。所有样本均在ICARDA病毒学实验室采用组织印迹免疫分析法(TBIA)检测特异性抗体。随机抽样结果表明,大麦黄矮病毒(BYDVPAV)在小麦和大麦中最常见(12.6%),其次是小麦矮病毒(WDV)(1.1%)。在有症状的样本中,BYDV-PAV也是最常见的(187个样本中有45个被感染:45/187),其次是WDV(23/187)、大麦黄条纹花叶病毒(BYSMV)(40/187)和玉米条纹病毒(MSV)(7/187)。在豆科田中,34份检测样本中最常见的是大豆矮缩病毒(SbDV) 13份(13/34),其次是豆叶卷病毒(BLRV)(12/34)、鹰嘴豆绿缩病毒(CpCSV)(5/34)和甜菜西黄病毒(BWYV)(4/34)。用特异性引物进行聚合酶链反应(PCR)扩增,确认血清学结果。这是利比亚首次报告谷物作物的WDV、MSV和BYSMV,以及豆类作物的BLRV、BWYV、SbDV和CpCSV。关键词:利比亚,谷物,豆类,病毒,TBIA, PCR
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引用次数: 0
Genetically Modified Crops, Production, Detection Methods and its Biosafety Implications: A Scientific Review 转基因作物、生产、检测方法及其生物安全意义:科学综述
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-40.3.260279
Nabila M. Ali Basha, Ahmad M. Abdul kader
Ali Basha, N.M. and A.M. Abdul Kader. 2022. Genetically Modified Crops, Production, Detection Methods and its Biosafety Implications: A Scientific Review. Arab Journal of Plant Protection, 40(3): 260-279. https://doi.org/10.22268/AJPP-40.3.260279 Ensuring food security and nutrition is critical for all countries in order to overcome the problems of hunger and malnutrition, taking into consideration the various current challenges of high population rate, social and political turmoil, and degradation of natural resources, forced migration and human disease pandemics. Agricultural biotechnology contributes in enhancing agricultural productivity, food security, and livelihoods. 25 years passed since the world introduced and embraced biotech crops in 1996. Such improved genetically modified (GM) crop varieties have many useful traits such as insect resistance, herbicide tolerance, resistance to biotic and abiotic stresses, in addition to improved nutrion value, and by adopting stringent science- scrutiny and safety measures. In this context, the Biosafety Cartagena Protocol was approved to ensure the safe handling, transfer and use of living organisms that have been modified using modern biotechnology. Socioeconomic and environmental benefits have been documented by credible and independent agencies around the world. Therefore, products derived from agricultural biotechnology, especially those used to manage agricultural pests, have become one of the world fastest growing agricultural trade commodities, providing food, feed, clothing, and eco-friendly biofuels. Not to mention the development of the genome editing technology using CRISPR/Cas9, which is another step closer to developing and cultivating new varieties of agricultural crops through the use of accurate, efficient and affordable techniques for genome editing. On the other hand, researchers have developed rapid and standardized methods for the detection of genetically modified plants and seeds to facilitate testing and monitoring genetic modification taking place at the global level in order to comply with the biosafety regulations and laws. Keywords: Biosafety, biosafety clearing-house (BCH), Cartagena protocol on biosafety, genetic engineering, genetically modified crops, GMCs, GMOs
阿里·巴沙,新墨西哥州和上午阿卜杜勒·卡德尔,2022年。转基因作物、生产、检测方法及其生物安全意义:科学综述。植物保护学报,40(3):260-279。https://doi.org/10.22268/AJPP-40.3.260279确保粮食安全和营养对所有国家都至关重要,以便克服饥饿和营养不良问题,同时考虑到目前的各种挑战,如高人口比率、社会和政治动荡、自然资源退化、被迫移徙和人类疾病大流行。农业生物技术有助于提高农业生产力、粮食安全和生计。自1996年世界引进并接受转基因作物以来,已经过去了25年。这种改良的转基因(GM)作物品种具有许多有用的特性,如抗虫、抗除草剂、抗生物和非生物胁迫,此外还具有更高的营养价值,并采用了严格的科学审查和安全措施。在这方面,《卡塔赫纳生物安全议定书》获得批准,以确保安全处理、转移和使用利用现代生物技术改造的活生物体。社会经济和环境效益已被世界各地可靠的独立机构证明。因此,来自农业生物技术的产品,特别是那些用于管理农业害虫的产品,已成为世界上增长最快的农业贸易商品之一,提供食品、饲料、服装和环保生物燃料。更不用说使用CRISPR/Cas9的基因组编辑技术的发展,通过使用准确、高效和负担得起的基因组编辑技术,它向开发和培育农作物新品种又迈进了一步。另一方面,研究人员开发了快速和标准化的转基因植物和种子检测方法,以促进在全球范围内进行的转基因检测和监测,以遵守生物安全法规和法律。关键词:生物安全,生物安全信息交换所,卡塔赫纳生物安全议定书,基因工程,转基因作物,转基因生物,转基因生物
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引用次数: 0
Toxicity Stress of the Durah Power Plant Ash and its Effect on the Alga Chlorococcum humicola (Naeg) Rabenhorst 1868 杜拉电厂灰渣的毒性胁迫及其对腐殖酸绿球藻(Naeg)的影响
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-040.2.188192
Noor A.Sh. Al-Naymi, Hanan A.S. Al-Naymi, M. Nashaat
Al-Naymi, N.A.Sh., H.A.S. AL-Nuaimi and M.R. Nashaat. 2022. Toxicity Stress of the Durah Power Plant Ash and its Effect on the Alga Chlorococcum humicola (Naeg) Rabenhorst 1868. Arab Journal of Plant Protection, 40(2): 188-192. https://doi.org/10.22268/AJPP-040.2.188192 This study illustrates the acute toxic effect of ash released from Durah power plant (DPP) on the biology of the phytoplankton species Chlorococcum humicola in Iraq. The results showed that the median lethal concentration for killing 50% of the Alga population (LC50) was 0.15 and 0.13 ppt (parts per thousand) for 24 and 48 hours exposure to crude ash concentrations, respectively. In contrast, no LC50 value was recorded for 72 and 96 hrs after exposure. The reduction in the optical density absorption value and the growth rate recorded was 0.083±0.121 cells for the highest ash concentration used, compared with 0.594±0.099 cells recorded for the control group. On the other hand, the doubling time for the control group was 1.16±0.652 an hour compared with 1.36±0.981 an hour recorded for 2 ppt ash exposure. The current study confirms that the crude ash concentrations tested had an adverse toxic effect on the biological parameters of the algal species Chlorococcum humicola in Iraq. Keywords: Algae, fly ash, pollutants, power plant, C. humicola
Al-Naymi N.A.Sh。, H.A.S. AL-Nuaimi和mr . Nashaat. 2022。杜拉电厂灰渣的毒性胁迫及其对腐殖酸绿球藻(Naeg)的影响植物保护学报,40(2):188-192。https://doi.org/10.22268/AJPP-040.2.188192本研究阐明了杜拉电厂(DPP)释放的灰对伊拉克浮游植物物种腐殖质绿球藻(Chlorococcum humicola)生物学的急性毒性作用。结果表明,粗灰分暴露24小时和48小时,对50%海藻种群的中位致死浓度(LC50)分别为0.15和0.13 ppt(千分之一)。与此相反,暴露后72和96小时LC50值未被记录。使用最高灰分浓度时,记录的光密度吸收值和生长率的降低为0.083±0.121个细胞,而对照组记录的光密度吸收值为0.594±0.099个细胞。另一方面,对照组的翻倍时间为1.16±0.652每小时,而2 ppt灰暴露组的翻倍时间为1.36±0.981每小时。目前的研究证实,所测试的粗灰分浓度对伊拉克腐殖质绿球藻的生物参数有不利的毒性作用。关键词:藻类;粉煤灰;污染物
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引用次数: 0
First Record of the Two Insects Megaselia halterata and Lycoriella ingenua that Attack Some Edible Mushrooms in Iraq and Assessing the Damage Caused by them 伊拉克几种食用菌的两种昆虫——halteria Megaselia halterata和Lycoriella ingenua首次记录及危害评估
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-40.3.209214
Abdallah Hassan, Abeer AlQaissi
Hassan, A.A. and A.R.M. Al-Qaissi. 2022. First Record of the Two Insects Megaselia halterata and Lycoriella ingenua that Attack Some Edible Mushrooms in Iraq and Assessing the Damage Caused by them. Arab Journal of Plant Protection, 40(3): 209-214. https://doi.org/10.22268/AJPP-40.3.209214 Invading insects were found in the fruiting bodies of the edible mushrooms Agaricus bisporus and Pleurotus spp. at the mushroom farm of the Faculty of Agriculture, University of Tikrit, through the presence of holes, necrosis and tunnels in the mushroom fruits. Infested fruiting bodies containing larvae were collected and insects were reared in the laboratory for the purpose of diagnosis. The phenotypic diagnosis was confirmed by molecular diagnosis based on the nucleotide sequence of the cytochrome oxidase C subunit 1 gene (in mitochondria). The two insects were identified as Megaselia halterata and Lycoriella ingenua and were registered in the NCBI database under the accession numbers MZ021516.1 and MZ021517.1, respectively. This is the first record of these two species in Iraq. The study also included a description of the damage caused by these two insects on the fruiting bodies of the mushrooms A. bisporus and Pleurotus spp. Keywords: Molecular identification, mushroom fly, Agaricus bisporus, Pleurotus spp
哈桑,匿名戒酒会和基地组织。2022. 伊拉克几种食用菌的两种昆虫——halteria Megaselia halterata和Lycoriella ingenua首次记录及危害评估。植物保护学报,40(3):209-214。https://doi.org/10.22268/AJPP-40.3.209214在提克里特大学农业学院的蘑菇农场,在食用菌Agaricus bisporus和Pleurotus sp.的子实体中发现了入侵昆虫,通过蘑菇果实中存在的洞,坏死和隧道。收集含幼虫的侵染子实体,在实验室饲养昆虫进行诊断。基于细胞色素氧化酶C亚基1基因(线粒体)核苷酸序列的分子诊断证实表型诊断。2种昆虫经鉴定为halterata meggaselia和Lycoriella ingenua,已在NCBI数据库中登记,登记号分别为MZ021516.1和MZ021517.1。这是这两个物种在伊拉克的首次记录。本文还描述了这两种昆虫对双孢蘑菇和平菇子实体的危害。关键词:分子鉴定;双孢蘑菇
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引用次数: 1
Infection and Egg Development of the Root-Knot Nematode Meloidogyne javanica on Oleander Nerium oleander 夹竹桃根结线虫在夹竹桃上的侵染与卵发育
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-40.4.293298
Rahma Mukhlis Adel, Firas Kadhim Al-Juboori
Adel, R.M. and F.K. Aljuboori. 2022. Infection and Egg Development of the Root-Knot Nematode Meloidogyne javanica on Oleander Nerium oleander. Arab Journal of Plant Protection, 40(4): 293-298. https://doi.org/10.22268/AJPP40.4.293298 The identification and first record of Nerium oleander infection with root-knot nematode M. javanica was confirmed by the PCR test using the pair of species-specific primers (Fjav and Rjav). Symptoms of the Oleander root-knot disease are similar to those caused by these nematodes on other flowering plants. The results of the study described the stages of embryo development inside the egg and until hatching under laboratory conditions. The difference in egg shape index ranged from 35.8 to 87.9, whereas the dominant egg shape index was between 44.5 and 48.8. Keywords: Nerium oleander, eggs, embryo development, root knot nematode, Meloidogyne javanica
阿德尔,R.M.和F.K. Aljuboori, 2022。夹竹桃根结线虫在夹竹桃上的侵染与卵发育。植物保护学报,40(4):293-298。https://doi.org/10.22268/AJPP40.4.293298采用Fjav和Rjav引物对夹竹桃根结线虫进行PCR检测,证实夹竹桃根结线虫感染的鉴定和首次记录。夹竹桃根结病的症状与这些线虫在其他开花植物上引起的症状相似。研究结果描述了胚胎在卵子内发育的各个阶段,直到在实验室条件下孵化。蛋形指数差异为35.8 ~ 87.9,优势蛋形指数差异为44.5 ~ 48.8。关键词:夹竹桃;卵;胚发育
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引用次数: 0
Survey and Identification of Some Eulophid Parasitoids (Hymenoptera) of Tomato Leaf Miner (Tuta absoluta) (Lepidoptera: Gelechiidae) Along the Syrian Coast 叙利亚沿岸番茄小叶蜂(Tuta absoluta)部分拟真寄生蜂(膜翅目:姬蜂科)的调查与鉴定
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-40.2.127139
Nabil abo Kaf, R. Youssef, Rafeek Abboud
Abo Kaf, N., R. Youssef and R. Aboud. 2022. Survey and Identification of Some Eulophid Parasitoids (Hymenoptera) of Tomato Leaf Miner (Tuta absoluta) (Lepidoptera: Gelechiidae) Along the Syrian Coast. Arab Journal of Plant Protection, 40(2): 127-139. https://doi.org/10.22268/AJPP-40.2.127139 The Eulophid (Hymenoptera: Chalcidoidea) parasitoids of tomato leaf miner (Tuta absoluta) were collected from tomato fields in Latakia and Tartus provinces of Syria during the period 2019-2020. The collected parasitoids were identified to subfamilies, genera and species, and found to belong to three subfamilies: Tetrastichinae, Entedoninae, Eulophinae, and 5 Genera and 14 species: Stenomesius japonicas, 3 species from the genus Stenomesius, Hemiptarsenus unguicellus, Pnigalio agraules, and 6 species from the genus Pnigalio, Neochrysocharis formosa and one species from the genus Elasmus. The species Stenomesius japonicas and three species of genus Stenomesius, Hemiptarsenus unguicellus, Pnigalio agraules and Neochrysocharis formosa were recorded for the first time in Syria on tomato leaf miner (Tuta absoluta) on tomato plants. The most widely spread of these species was N. formosa (46.9%) and S. japonicus and 3 species from genus Stenomesius (45.7%), followed by 6 species from genus Pnigalio (4.35%), Elasmus sp. (1.37%), P. agraules (0.69%), and H. unguicellus (0.46%). Keywords: Tuta absoluta, Parasitoids, Eulophidae, Syria.
Abo Kaf, N., R. Youssef和R. Aboud., 2022。叙利亚沿岸番茄小叶蜂(Tuta absoluta)部分拟真寄生蜂(膜翅目:姬蜂科)的调查与鉴定。植物保护学报,40(2):127-139。https://doi.org/10.22268/AJPP-40.2.127139 2019-2020年在叙利亚拉塔基亚省和塔尔图斯省番茄田采集了番茄叶螨(Tuta absoluta)的寄生蜂(膜翅目:蜂科)。经鉴定,收集到的寄生蜂共分属4个亚科、6个属、1个种,其中分属于四扇蝽亚科、扇蝽亚科、细纹蝽亚科3个属、14种;日本细纹蝽5个属、14种,细纹蝽属3种,台湾细纹蝽属6种,新黄斑蝽属1种。在叙利亚首次在番茄植株的番茄叶螨(Tuta absoluta)上记录到日本小蠹蛾(Stenomesius japonicas)和小蠹蛾属(Hemiptarsenus unguicellus)、小蠹蛾属(Pnigalio agraules)和台湾新黄斑蝽属(Neochrysocharis formosa) 3种。分布最广的是台湾褐家鼠(46.9%)和日本褐家鼠(46.9%),窄尾褐家鼠属3种(45.7%),其次是褐家鼠属6种(4.35%)、绿绒家鼠(1.37%)、绿绒家鼠(0.69%)和带甲褐家鼠(0.46%)。关键词:绝对大蠊,拟寄生蜂,蠓科,叙利亚
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引用次数: 0
ECOLOGY Susceptibility of Apple Varieties to Tetranychus urticae Koch and Panonychus ulmi Koch and its Relation to the Nutrient Contents and Spider Mite Predators in Apple Trees 苹果品种对荨麻叶螨和乌尔全爪螨的生态敏感性及其与苹果树营养成分和蜘蛛螨捕食者的关系
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-40.2.119126
Jihan Abdallah, Mageda Mofleh, L. Aslan
El-Abdallah, J., M. Muflih and L.H. Aslan. 2022. Susceptibility of Apple Varieties to Tetranychus urticae Koch and Panonychus ulmi Koch and its Relation to the Nutrient Contents and Spider Mite Predators in Apple Trees. Arab Journal of Plant Protection, 40(2): 119-126. https://doi.org/10.22268/AJPP-40.2.119126 The susceptibility to T. urticae and P. ulmi of eight apple varaietis were studied in southern Syria (Sweida) from 2018 to 2020. Significant differences of susceptibility to the two species between apple varieties, in addition to significant differences of apple-leaf nutrients content (NPK) were found. Results obtained showed that apple-leaf nutrients content affect the susceptibility to the two spider mite species differently. Principal component analysis (PCA) showed positive correlation to P. ulmi with apple-leaf contents of potassium and negative correlation with apple-leaf contents of nitrogen and showed positive correlation between T. urticae numbers and nitrogen and phosphorous content. T. urticae had a weak correlation with potassium apple-leaf content in 2019. Cluster analysis showed that apple varieties were distributed in three clusters based on the studied factors in 2020. The first cluster included the varieties Top Red, Starking Delicious and Starking Red Delicious which were the most sensitive to P. ulmi. The second cluster included Golden Delicious, Golden 972 and Granny Smith, and those were less sensitive to P. ulmi and the third cluster included Ozark Gold and Royal Red and these varieties were moderately sensitive to P. ulmi. The effects of studied factors were clearer on P. ulmi than on T. urticae. Keywords: Apple, T. urticae, P. ulmi, Phytoseiidae, Principal component analysis, PCA.
El-Abdallah, J., M. Muflih和L.H. Aslan, 2022。苹果品种对荨麻叶螨和乌尔全爪螨的敏感性及其与苹果树营养成分和蜘蛛螨捕食者的关系植物保护学报,40(2):119-126。https://doi.org/10.22268/AJPP-40.2.119126 2018 - 2020年在叙利亚南部(瑞典)研究了8种苹果品种对荨麻疹和乌尔米病的敏感性。苹果品种间对这两种病菌的敏感性差异显著,叶片养分含量(NPK)差异显著。结果表明,苹果叶片养分含量对两种螨的敏感性有不同的影响。主成分分析(PCA)表明,绿僵菌数量与苹果叶片钾含量呈正相关,与苹果叶片氮含量呈负相关,与玉米叶片氮、磷含量呈正相关。2019年,荨麻疹与苹果叶片钾含量呈弱相关。聚类分析表明,2020年苹果品种分布在3个聚类中。第一个聚类包括对乌尔米病菌最敏感的品种Top Red、Starking Delicious和Starking Red Delicious。第二集群包括金冠、金972和史密斯奶奶,对乌尔米病不太敏感;第三集群包括奥扎卡金和皇家红,这些品种对乌尔米病中等敏感。所研究的因素对乌尔米菌的影响比对荨麻疹菌的影响更明显。关键词:苹果,荨麻,乌尔米,植物科,主成分分析,主成分分析
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引用次数: 0
Control of Fusarium Wilt Disease of Cucumber Using Rhizospheric Antagonistic Fungi 根际拮抗真菌防治黄瓜枯萎病
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-040.1.062069
Abdulnabi A. Matrood, A Rhouma, T. F. Mohammed
Fusarium wilt disease caused by Fusarium oxysporum f. sp. cucumerinum is a severe disease of cucumbers causing yield losses worldwide. Various experiments were conducted to assess the antifungal potential of fungal bio-agents isolated from soil and cucumber seed (bio-priming). Trichoderma koningii seemed to be the most effective bio-agent against F. oxysporum f. sp. cucumerinum with mycelial inhibition rate above 90%, followed by Aspergillus niger (87.70%) under laboratory conditions. Results showed that T. koningii was found effective to enhance the germination rate (95.07%). In greenhouse experiments, T. koningii applied preventively on cucumber inoculated with F. oxysporum f. sp. cucumerinum generated the lowest disease severity index (0.42), mortality (13.33%) and wilting rate (23.33%) and the highest fresh (0.857 g) and dry (0.180 g) weight of root, and root volume (2.04 cm3 ). Based on these results, T. koningii applied preventively against F. oxysporum f. sp. cucumerinum on cucumber plants could be recommended as a biocontrol agent. However, although A. niger was effective against the tested phytopathogen, it is not recommended for biological control due to its carcinogenic properties. Keywords: Biological control, Cucumis sativus, Fusarium oxysporum f. sp. cucumerinum, antifungal potential.
黄瓜枯萎病(Fusarium oxysporum f. sp. cucumerinum)是一种严重危害黄瓜产量的病害。从土壤和黄瓜种子中分离真菌生物制剂(生物启动法),对其抑菌潜力进行了研究。实验条件下,科宁木霉对黄瓜尖孢菌的抑菌率最高,达到90%以上,其次是黑曲霉,抑菌率为87.70%。结果表明,koningii可有效提高发芽率(95.07%)。在温室试验中,对接种了尖孢镰刀菌的黄瓜进行预防施用,其病害严重指数最低(0.42),死亡率最低(13.33%),萎蔫率最低(23.33%),根系鲜重最高(0.857 g),干重最高(0.180 g),根系体积最高(2.04 cm3)。综上所述,koningii对黄瓜尖孢镰刀菌具有一定的防治作用。然而,尽管黑曲霉对所测试的植物病原体有效,但由于其致癌特性,不建议用于生物防治。关键词:生物防治;黄瓜;黄瓜尖孢镰刀菌;
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引用次数: 0
Effect of Rhizophagus irregularis on Growth of Saffron (Crocus sativus L.) in Eastern Morocco 不规则根噬菌对摩洛哥东部藏红花生长的影响
Pub Date : 2022-01-01 DOI: 10.22268/ajpp-040.2.182187
M. Rimani, Ibtissam Mzbari, K. Charif, Zoheir Chafik, E. Kharmach
Rimani, M., I. Mzabri, K. Charif, Z. Chafik and E. Kharmach. 2022. Effect of Rhizophagus irregularis on Growth of Saffron (Crocus sativus L.) in Eastern Morocco. Arab Journal of Plant Protection, 40(2): 182-187. https://doi.org/10.22268/AJPP-040.2.182187 Arbuscular mycorrhizal fungi (AMF) form mutualistic relationships with plant roots and can act as bio-fertilizers. In the same perspective, a study was conducted to investigate the possibility of a possible constitutive association of the arbuscular mycorrhizal fungus Rhizophagus irregularis L. (previously named Glomus intraradices) with saffron. The trial was conducted in the field at the experimental station of the Faculty of Sciences of Oujda (Morocco) by applying 3 doses of R. irregularis inoculum: 2, 4 and 6 ml per corm (T1, T2 and T3 treatments). Six months after planting, morphological, biochemical and mycorrhization parameters were measured. The results showed that the inoculation of saffron roots by R. irregularis was successful, which induced a significant increase in the number of leaves, the weight of stigmas and the percentage of daughter corms with large diameter. Similarly, the total chlorophyll content was increased, the highest value was recorded for the T3 treatment in April (0.04 mg/g MF), with an increase of 25% compared to the control. Keywords: Arbuscular mycorrhizal fungus (AMF), inoculum, root colonization, total chlorophyll content, Rhizophagus irregularis, saffron.
Rimani, M., I. Mzabri, K. Charif, Z. Chafik和E. Kharmach. 2022。不规则根噬菌对摩洛哥东部藏红花生长的影响。植物保护学报,40(2):182-187。https://doi.org/10.22268/AJPP-040.2.182187丛枝菌根真菌(AMF)与植物根系形成共生关系,可作为生物肥料。在同样的观点下,研究人员进行了一项研究,以调查丛枝菌根真菌Rhizophagus irregularis L.(以前称为Glomus intraradices)与藏红花可能构成关联的可能性。该试验在Oujda(摩洛哥)科学学院实验站的田间进行,采用3剂不规则稻接种剂:每粒玉米2、4和6毫升(T1、T2和T3处理)。种植6个月后,测定形态、生化和菌根参数。结果表明:红花根茎接种成功,叶片数、柱头重量和大直径子珠百分率均显著增加;叶绿素总含量也有所增加,T3处理在4月份达到最高值(0.04 mg/g MF),比对照提高了25%。关键词:丛枝菌根真菌(AMF),接种量,根定植,总叶绿素含量,不规则食根菌,藏红花
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Arab Journal for Plant Protection
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