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Detection of leaf disease in tomato plants using a lightweight parallel deep convolutional neural network 基于轻量级并行深度卷积神经网络的番茄叶片病害检测
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-26 DOI: 10.1080/03235408.2023.2216359
Rashmi Deshpande, Hemant Patidar
Abstract Plant diseases and poisonous insects are major threats to agriculture. As a result, detecting and diagnosing these illnesses as soon as feasible is critical. The ongoing development of major deep learning techniques has substantially aided in the diagnosis of plant leaf diseases, providing a potent instrument with incredibly exact results. Deep learning algorithms, on the other hand, are dependent on the quality and quantity of labelled data used for training. The lightweight parallel deep convolutional neural network is described in this study for detecting plant leaf disease. In addition, the Generative Adversarial Neural Network is introduced for creating synthetic data in order to overcome the data scarcity problem caused by uneven dataset size. The experimental results for two-class, six-class and ten-class disease identification of tomato plant samples from the Plant Village dataset are provided. The effectiveness of the proposed model is assessed using numerous performance measures, including accuracy, recall, precision and F1-score, and compared to known state-of-the-art approaches for tomato plant leaf disease detection. The proposed system provides better accuracy (99.14%, 99.05%, 98.11% accuracy for the 2-class, 6-class and 10-class) for tomato leaf disease detection compared with traditional existing approaches.
植物病害和毒虫是危害农业的主要威胁。因此,尽快发现和诊断这些疾病至关重要。主要深度学习技术的持续发展极大地帮助了植物叶片疾病的诊断,提供了一种强有力的工具,具有令人难以置信的精确结果。另一方面,深度学习算法依赖于用于训练的标记数据的质量和数量。提出了一种轻型并行深度卷积神经网络用于植物叶片病害检测的方法。此外,为了克服数据集大小不均匀导致的数据稀缺性问题,引入了生成对抗神经网络来创建合成数据。给出了植物村数据集中番茄植株样本的二级、六级和十级病害鉴定的实验结果。该模型的有效性评估使用了许多性能指标,包括准确性、召回率、精度和f1分数,并与已知的最先进的番茄植物叶片病害检测方法进行了比较。与传统方法相比,该系统对番茄叶片病害的检测准确率分别为99.14%、99.05%、98.11%(2级、6级和10级)。
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引用次数: 0
Talaromyces omanensis and Aspergillus fumigatus endophytic fungi suppress Pythium aphanidermatum and its induced damping-off diseases of cucumber and radish 阿曼Talaromyces omanensis和烟曲霉内生真菌抑制无叶腐霉及其诱导的黄瓜和萝卜抗病性
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-26 DOI: 10.1080/03235408.2023.2216350
Boshra A. Halo, Rashid Al-Yahyai, A. Al-Sadi
Abstract Damping-off diseases caused huge losses in crop production in numerous countries. Pythium aphanidermatum represents a major cause of these diseases. In the present investigation, two endophytic fungi Talaromyces omanensis and Aspergillus fumigatus isolated from a desert plant, Rhazya stricta, were investigated for their efficiency in suppressing P. aphanidermatum and its stimulated damping-off diseases in cucumber and radish. The results proved the ability of both endophytes to inhibit P. aphanidermatum growth in both solid and liquid forms, inhibit its oospore production, induce abnormal patterns in its hyphae and suppress its induced damping-off diseases. Moreover, T. omanensis produced an inhibition zone area against P. aphanidermatum and enhanced the fresh weight of cucumber and length, fresh weight and dry weight of radish. Further, A. fumigatus caused cellular leakage in P. aphanidermatum mycelium. The previous results were interpreted through the secretion of β-1,3-glucanase, cellulase and siderophore by both endophytic fungi.
在许多国家,退潮病给农作物生产造成了巨大损失。蛇皮霉是这些疾病的主要病因。本文研究了从沙漠植物拉兹亚(Rhazya stricta)中分离得到的两种内生真菌阿曼塔芳菌(Talaromyces omanensis)和烟曲霉(Aspergillus fumigatus)对黄瓜和萝卜中蛇皮霉(P. aphanidermatum)及其诱发的消湿病害的抑制作用。结果证明,这两种内生菌均能抑制假单胞菌的固体和液体生长,抑制其卵孢子的产生,诱导其菌丝的异常模式,抑制其诱导的阻尼病。对黄瓜鲜重、萝卜长、鲜重和干重均有显著提高,对黄瓜鲜重和萝卜干重均有显著提高。此外,烟曲霉还会引起假单胞菌菌丝的细胞渗漏。上述结果可以通过内生真菌分泌β-1,3-葡聚糖酶、纤维素酶和铁载体来解释。
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引用次数: 0
List of reviewers 审稿人名单
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-09 DOI: 10.1080/03235408.2023.2213054
S. Khanizadeh
Peer review is fundamental for evaluating MS submitted to Archives of Phytopathology and Plant Protection (APPP) journal and we are fortunate to have the acceptance of the following experts for volunteering their time to review the submitted articles with genuine interest. Providing such a voluntary service, under current circumstances is truly a devotion to the advancement of research in the field. The quality of APPP journal depends on dedicated reviewers such as these and I would like to take this opportunity to express my appreciation to them for accepting this extra task in their already very busy schedules. I cannot thank you enough for your contribution. Shahrokh Khanizadeh appp-editor-in-chief@outlook.com
同行评议是评估提交给植物病理学和植物保护档案(APPP)期刊的MS的基础,我们很幸运地接受了以下专家自愿花时间对提交的文章进行真正感兴趣的评议。在目前的情况下,提供这样的志愿服务确实是对该领域研究进步的一种奉献。APPP期刊的质量取决于这些专业的审稿人,我想借此机会向他们表示感谢,他们在已经非常繁忙的日程中接受了这项额外的任务。我对你的贡献感激不尽。Shahrokh Khanizadeh appp-editor-in-chief@outlook.com
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引用次数: 0
Use of Ganoderma lucidum extract to elevate the resistance in chickpea against the Fusarium oxysporum f. sp. ciceris 利用灵芝提取物提高鹰嘴豆对尖孢镰刀菌的抗性
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-09 DOI: 10.1080/03235408.2023.2207955
C. Singh, D. Vyas
Abstract Biological based formulation for inducing systematic resistance in the crop is a sustainable measure to control plant disease by utilizing the natural defence system of the host plant and meet the strategic needs of pesticide application for agricultural products worldwide. The crude extract of Ganoderma lucidum was used to induce systematic resistant in the chickpeas against the Fusarium wilt caused by soil-borne fungus Fusarium oxysporum (Schletend: Fr) f. sp. ciceri (Padwick) (FOC). The formulation was made using a crude extract of Ganoderma lucidum and an emulsion (water in oil: W/O); different dilutions of the formulation were made and applied to the chickpea and then challenged with the FOC. After the incubation with FOC, the chickpea was assayed for the enzyme involved in Phenylpropanoid pathways, i.e. Peroxidase (PO), Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL). The activity levels of these enzymes were changed in chickpea treated with the formulations as compared to the control (water treated chickpea). The GC-MS analysis confirmed bioactive compounds responsible for the enhanced level of enzyme in chickpea. The findings of the study would lead to the development of a bio-formulation based inducer of resistance molecules in the chickpea against the Fusarium wilt.
摘要诱导作物系统抗性的生物制剂是利用寄主植物的自然防御系统控制植物病害的可持续措施,满足了全球农产品农药应用的战略需求。利用灵芝粗提物诱导鹰嘴豆对土传真菌尖孢镰刀菌(Fusarium oxysporum,Schletend:Fr)f.sp.ciceri(Padwick)(FOC)引起的枯萎病产生系统抗性。该制剂使用灵芝的粗提取物和乳液(油包水:W/O)制备;制备不同稀释的制剂并将其应用于鹰嘴豆,然后用FOC激发。在与FOC孵育后,测定鹰嘴豆中参与苯丙烷途径的酶,即过氧化物酶(PO)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)。与对照(水处理鹰嘴豆)相比,用制剂处理的鹰嘴豆中这些酶的活性水平发生了变化。GC-MS分析证实了鹰嘴豆中酶水平升高的生物活性化合物。这项研究的结果将导致开发一种基于生物制剂的鹰嘴豆抗枯萎病分子诱导剂。
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引用次数: 1
Effect of culture filtrates of Alternaria alternata on seed germination and seedling growth of sesame 链格孢培养滤液对芝麻种子萌发和幼苗生长的影响
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-09 DOI: 10.1080/03235408.2023.2212417
Gulshan Bibi, Brian Gagosh Nayyar, M. Ajmal, Asma Mehak, Wajiha Seerat, M. Shahbaz, T. Mukhtar, A. Akram
Abstract Sesame (Sesamum indicum L.) is a major oilseed crop in Pakistan, possessing high nutritional and pharmacological properties. Sesame is highly susceptible to pathogenic fungi such as Alternaria alternata. In the present study, metabolites extracted from culture filtrates of Alternaria alternata using hexane, chloroform, ethyl acetate, and petroleum ether solvents were evaluated for activity on seed germination and seedling health of sesame. Seeds treated with solvent-extracted metabolites from culture filtrates of A. alternata showed low germination as compared to control. Maximum reduction in germination was reported in seeds treated with petroleum ether culture filtrate followed by ethyl acetate, hexane, and chloroform. Shoot length and root length were also reduced in seeds treated with petroleum ether culture filtrate followed by ethyl acetate, hexane, and chloroform. Seedling treated with solvent-extracted metabolites from culture filtrates of A. alternata showed various symptoms including chlorosis, necrosis, discoloration, and wilting. From the present study, it is concluded that A. alternata contains certain metabolites which can reduce seed germination and are destructive for seedling stage of sesame. This is a baseline study to evaluate highly active metabolites which can be managed further to decrease sesame yield loss.
摘要芝麻(Sesamum indicum L.)是巴基斯坦的主要油料作物,具有较高的营养和药理特性。芝麻对链格孢等病原真菌高度敏感。在本研究中,用己烷、氯仿、乙酸乙酯和石油醚溶剂从链格孢培养滤液中提取的代谢产物对芝麻种子发芽和幼苗健康的活性进行了评估。与对照相比,用来自A.alternata培养滤液的溶剂提取的代谢物处理的种子显示出低发芽率。据报道,用石油醚培养滤液、乙酸乙酯、己烷和氯仿处理的种子发芽率最高。用石油醚培养滤液、乙酸乙酯、己烷和氯仿处理的种子中,茎长和根长也减少。用溶剂提取的A.alternata培养滤液中的代谢产物处理的幼苗表现出各种症状,包括褪绿、坏死、变色和枯萎。研究结果表明,交流A.alternata含有一定的代谢产物,这些代谢产物会降低种子的发芽率,对芝麻幼苗期具有破坏性。这是一项评估高活性代谢产物的基线研究,可以进一步管理这些代谢产物以减少芝麻产量损失。
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引用次数: 0
Outstanding editors 优秀的编辑
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-09 DOI: 10.1080/03235408.2023.2213053
S. Khanizadeh
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引用次数: 0
Analysis of viruses infecting Cape gooseberry (Physalis peruviana) in southwestern Antioquia (Colombia) suggests a new member of the genus Trichovirus 对感染哥伦比亚安蒂奥基亚西南部醋栗(Physalis peruviana)的病毒的分析表明,毛病毒属中有一个新成员
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-09 DOI: 10.1080/03235408.2023.2216342
Michelle Bacca, Mónica Higuita, Andrea Restrepo, Yuliana Gallo, M. Marín, P. Gutiérrez
Abstract Cape gooseberry (Physalis peruviana) is one of the most important crops in Antioquia (Colombia). However, little is known about the viruses circulating in Cape gooseberry in the southwestern region of the province. In this work, the prevalence of seven viruses (PVY, PVS, PVX, TaLMV, PhyVNV, CGIV-1, and PMTV) was tested by RT-qPCR on seed samples, symptomatic and asymptomatic plants from commercial fields from this region, and virus genomes were investigated using high-throughput sequencing (HTS). The results showed the detection of all seven viruses in at least one sample. PMTV was the most prevalent virus as it was found in six seed samples, eight asymptomatic, and ten symptomatic samples. HTS analysis revealed the presence of a new virus species closely related to the genus Trichovirus and tentatively named as Cape gooseberry associated trichovirus (CgaTV). Near full-length genome sequences were obtained for P. peruviana infecting isolates of TaLMV, PhyVNV, and PVY.
摘要开普醋栗(Physalis peruviana)是安蒂奥基亚(哥伦比亚)最重要的作物之一。然而,人们对在该省西南部地区的醋栗角传播的病毒知之甚少。在这项工作中,通过RT-qPCR检测了七种病毒(PVY、PVS、PVX、TaLMV、PhyVNV、CGIV-1和PMTV)在该地区商业田地的种子样本、有症状和无症状植物上的流行率,并使用高通量测序(HTS)研究了病毒基因组。结果显示,至少在一个样本中检测到了所有七种病毒。PMTV是最流行的病毒,因为它在6个种子样本、8个无症状样本和10个有症状样本中被发现。HTS分析显示,存在一种与毛病毒属密切相关的新病毒,暂定名为开普醋栗相关毛病毒(CgaTV)。获得了秘鲁P.peruviana感染TaLMV、PhyVNV和PVY分离株的近全长基因组序列。
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引用次数: 1
Incidence of white mould of bean and characterization of its causal pathogen, Sclerotinia sclerotiorum in Kashmir valley, India 印度克什米尔谷地蚕豆白霉病发病及病原菌菌核病鉴定
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-05-09 DOI: 10.1080/03235408.2023.2213396
Nayeema Jan, M. Bhat, A. Wani, M. Malik, Meesa Jan
Abstract White mould caused by Sclerotinia sclerotiorum affects the quality and quantity of bean crop. The aims of this study were to evaluate the incidence and severity of white mould disease in four districts of Kashmir valley and to characterize the morphological variability of 60 Sclerotinia sclerotiorum isolates from bean plants. Three bean fields per village and five villages per district were chosen randomly for sampling. The highest incidence among surveyed areas was found in Ganderbal (37.94%) and lowest in Shupiyan (19.98%). The disease severity was highest in district Ganderbal (15.54%) and lowest in district Shupiyan (7.94%). Single spore method was used to isolate the fungus. Sixty isolates of the causal organism were isolated from bean plants collected from the surveyed areas and pathogenecity test was carried out following Koch’s postulates. Morphological characters of the different isolates, like number of sclerotia per Petri plate, time required for formation of the first sclerotium and average weight and shape of the sclerotia were studied. Among sixty isolates of S. sclerotiorum isolated from bean, it was observed that 25% of the isolates had abundant mycelium, 25% had irregular mycelium, half of the isolates had thin mycelium. 75% of the isolates had white colour and 25% had beige coloration. 25% of the isolates had a round shape, and 75% had a diverse shape. Sclerotia were detected in 50% of the cases in a regular pattern along the colony edge, 25% spread throughout the colony, and 25% in concentric circles. The mean number of sclerotia per colony ranged from 11.68 (SS55) to 32.32 (SS26). The mean number of days for formation of the first sclerotium ranged from 9.3 (SS30) to 17.7 (SS60). The average weight of sclerotia ranged from 0.0918 (SS58) to 0.2475 (SS21). The variation in disease incidence across different sites and the morphological variation of the isolates can be due to differences in genetic constitution as well as environmental conditions.
摘要由菌核菌引起的白霉病影响豆制品的质量和产量。本研究的目的是评估克什米尔山谷4个地区白霉病的发病率和严重程度,并对60株从豆类植物中分离出来的菌核菌核菌进行形态变异表征。每个村随机抽取3块豆田,每个区随机抽取5块豆田进行抽样。调查地区中发病率最高的是甘丹巴尔(37.94%),最低的是舒皮燕(19.98%)。发病严重程度以甘丹巴尔区最高(15.54%),疏皮岩区最低(7.94%)。采用单孢子法分离真菌。从调查地区收集的豆类植物中分离出60株病原菌,并按照科赫原理进行了致病性试验。研究了不同菌株菌核的形态特征,如每个培养皿的菌核数、第一个菌核形成所需的时间以及菌核的平均重量和形状。60株蚕豆菌丝体分离株中,菌丝体丰富的占25%,菌丝体不规则的占25%,菌丝体薄的占一半。75%的菌株呈白色,25%为米色。25%的菌株呈圆形,75%的菌株形状多样。在50%的病例中,菌核沿菌落边缘呈规则模式,25%分布在整个菌落中,25%呈同心圆状。平均菌核数为11.68 ~ 32.32 (SS26)。第一菌核形成的平均天数为9.3天(SS30) ~ 17.7天(SS60)。菌核平均质量为0.0918 (SS58) ~ 0.2475 (SS21)。不同位点的疾病发病率的差异和分离株的形态差异可能是由于遗传构成和环境条件的差异。
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引用次数: 0
Investigations on black root rot disease of mulberry caused by Lasiodiplodia theobromae 桑树黑腐病的防治研究
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-04-21 DOI: 10.1080/03235408.2023.2216367
S. Vijay, P. Renukadevi, P. Mangammal
Abstract Mulberry is an important food plant for domesticated silkworm (Bombyx mori L.). Among several root rot diseases reported in mulberry, black root rot caused by Lasiodiplodia theobromae has now emerged as a major disease. The survey was conducted and black root rot incidence was found to be maximum (51.7%) in Coimbatore district and minimum (14.1%) in Erode district. On PDA, L. theobromae covered Petri plate within three days with cottony white fluffy and slowly turned to black colour. All the four isolates were identified by molecular characterisation through PCR with ITS region and sequences were deposited in NCBI database. Among biocontrol agents screened, Trichoderma asperellum TRI 15 and T. virens TRI 37 were found to be inhibitory with 67.7% and 66.6% reduction in mycelial growth. Five mulberry varieties were screened, variety G2 showed resistance and V1 was highly susceptible.
摘要桑树是家蚕的重要食用植物,在报道的几种桑树根腐病中,黑根腐病已成为主要病害之一。调查发现,哥印拜陀区黑根腐病发病率最高(51.7%),埃罗德区最低(14.1%)。在PDA上,L.theobromae在三天内用棉白色绒毛覆盖培养皿,并慢慢变为黑色。所有四个分离株均通过ITS区的PCR分子特征鉴定,并将序列存储在NCBI数据库中。在筛选的生防剂中,木霉TRI 15和T.virens TRI 37对菌丝生长的抑制率分别为67.7%和66.6%。筛选出5个桑树品种,G2表现出抗性,V1表现出高度感病性。
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引用次数: 0
Overview of sugar beet leaf spot disease caused by Cercospora beticola Sacc 甜菜Cercospora beticola Sacc引起的甜菜叶斑病综述
IF 1 Q3 PLANT SCIENCES Pub Date : 2023-04-21 DOI: 10.1080/03235408.2023.2216356
Z. El Housni, Tahiri Abdessalem, N. Radouane, S. Ezrari, Abderrahim Zegoumou, A. Ouijja
Abstract Cercospora leaf spot induced by the fungus Cercospora beticola Sacc is the most important leaf disease of sugar beet (Beta vulgaris L.) in the world. Managing this enemy requires an integrated approach combining farming practices, resistant varieties, and chemical treatments. Indeed, chemical treatments against fungi provide control of the disease but generate another problem; the resistance to fungicidal molecules. Studies have detected the mutation points responsible for the resistance traits of this pathogen against the main fungicide groups (MBC, DMI, QoI). From the perspective of managing the resistance, alternative control methods such as the use of antagonists (fungi and bacteria).
摘要甜菜尾孢菌(Cercospora beticola Sacc)诱发的尾孢叶斑病是世界上最重要的甜菜叶病。管理这种敌人需要一种综合的方法,结合农业实践、抗性品种和化学处理。事实上,针对真菌的化学治疗可以控制疾病,但会产生另一个问题;对杀真菌分子的抗性。研究已经检测到了导致该病原体对主要杀菌剂群体(MBC、DMI、QoI)的抗性特征的突变点。从管理耐药性的角度来看,替代控制方法,如使用拮抗剂(真菌和细菌)。
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引用次数: 0
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Archives of Phytopathology and Plant Protection
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