Pub Date : 2023-08-27DOI: 10.1080/03235408.2023.2267664
Kh Nur E. Sadid, Md. Anowar Hossain, Arifur Rahman Munshi, Md. Rezaul Karim
AbstractPreliminary survey revealed that 30–40% of Cavendish banana plants were affected with leaf spot disease. Diseased leaf samples were sterilized with 3% sodium hypochlorite solution for 2 min and cultured on PDA media at 25 °C for 15 days followed by microscopic examination. Combined morphological, cultural and microscopic examinations identified two causal agents designated here as BaFI-1 and BaFI-2. Further, BLASTn search using internal transcribed spacer (ITS) rDNA sequences of the isolates also revealed that BaIF-1 and BaFI-2 had 97.13% and 100% homology with Nigrospora sphaerica and Neocordana musae, respectively; which were already reported from Indian and Spanish region. Pathogenecity tests were conducted using 2 months old banana seedlings inoculated with isolated spores in the polyhouse, which addressed Koch’s postulates. To our knowledge, this is the first report confirming Nigrospora sphaerica and Neocordana musae to be the causal agents of leaf spot disease of Cavendish banana in Bangladesh.Keywords: BananaMusa sppITS sequencesFungiLeaf spot disease Ethical approvalThis research work was carried out solely on plants. There is no ethical issue.Disclosure statementNo potential conflict of interest was reported by the authors.Data availability statementThe data that support the findings of this study are available in NCBI GenBank in the following URLs: https://www.ncbi.nlm.nih.gov/nuccore/MN966684; https://www.ncbi.nlm.nih.gov/nuccore/MT241255Additional informationFundingThis work was supported by Faculty of Science, University of Rajshahi.
{"title":"First detection, isolation and molecular characterization of banana leaf spot diseases caused by <i>Nigrospora sphaerica</i> and <i>Neocordana musae</i> in Bangladesh","authors":"Kh Nur E. Sadid, Md. Anowar Hossain, Arifur Rahman Munshi, Md. Rezaul Karim","doi":"10.1080/03235408.2023.2267664","DOIUrl":"https://doi.org/10.1080/03235408.2023.2267664","url":null,"abstract":"AbstractPreliminary survey revealed that 30–40% of Cavendish banana plants were affected with leaf spot disease. Diseased leaf samples were sterilized with 3% sodium hypochlorite solution for 2 min and cultured on PDA media at 25 °C for 15 days followed by microscopic examination. Combined morphological, cultural and microscopic examinations identified two causal agents designated here as BaFI-1 and BaFI-2. Further, BLASTn search using internal transcribed spacer (ITS) rDNA sequences of the isolates also revealed that BaIF-1 and BaFI-2 had 97.13% and 100% homology with Nigrospora sphaerica and Neocordana musae, respectively; which were already reported from Indian and Spanish region. Pathogenecity tests were conducted using 2 months old banana seedlings inoculated with isolated spores in the polyhouse, which addressed Koch’s postulates. To our knowledge, this is the first report confirming Nigrospora sphaerica and Neocordana musae to be the causal agents of leaf spot disease of Cavendish banana in Bangladesh.Keywords: BananaMusa sppITS sequencesFungiLeaf spot disease Ethical approvalThis research work was carried out solely on plants. There is no ethical issue.Disclosure statementNo potential conflict of interest was reported by the authors.Data availability statementThe data that support the findings of this study are available in NCBI GenBank in the following URLs: https://www.ncbi.nlm.nih.gov/nuccore/MN966684; https://www.ncbi.nlm.nih.gov/nuccore/MT241255Additional informationFundingThis work was supported by Faculty of Science, University of Rajshahi.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135182247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-27DOI: 10.1080/03235408.2023.2254675
Marwa E. Barghout, Elham A. Sammour
AbstractTwo types of formulations, emulsifiable concentrates (ECs) and nano-emulsions (NEs), were conducted to enhance the activity of the Rheum palmatum L. and Juniperus virginiana L. essential oils (EOs). The efficacy of EOs, ECs, and NEs was evaluated under laboratory and semi-field conditions against Tetranychus urticae Koch eggs and females. In addition, assessing their selectivity towards the predatory mite, Phytoseiulus persimilis Athias-Henriot. The tested ECs and NEs showed toxicity against T. urticae females higher than bulk oils, and all caused 100% un-hatchability of T. urticae eggs. With the exception of EOs and R. palmatum EC, neither the prepared ECs nor NEs showed any phytotoxicity symptoms on treated plants. The residual effects of tested materials were dissipated over time. Juniperus virginiana EO, EC and NE were compatible with P. persimilis. Tested ECs and NEs can aid in more sustainable crop management, to develop low-risk solutions to replace the use of pesticides.Keywords: Essential oilsformulationphytoseiid mitesselectivityTetranychus urticaetoxicity AcknowledgmentsWe are sincerely thanking Mr. Khaled A. Dawoud, technician, Pests and Plant Protection Department (NRC), for his valuable assistance in lab preparations.Disclosure statementThe authors declare there is no conflict of interest.Additional informationFundingThis work was funded by the National Research Centre (NRC) project No. 13050117.
摘要采用乳化浓缩液(ECs)和纳米乳液(NEs)两种剂型对棕榈大黄(Rheum palmatum L.)和杜松(Juniperus virginia L.)精油的活性进行了研究。在实验室和半现场条件下，评价了EOs、ECs和NEs对荨麻疹叶螨卵和雌虫的杀灭效果。此外，还对其对掠食性螨的选择性进行了评价。经检测的ECs和NEs对荨麻疹雌螨的毒力均高于散装油，且均能使荨麻疹卵100%不孵化。制备的ECs和NEs对处理植株均未表现出任何植物毒性症状。测试材料的残余效应随着时间的推移而消散。维吉尼亚杜松EO、EC和NE与persimilis均有亲和性。经过测试的ECs和NEs可以帮助更可持续的作物管理，开发低风险的解决方案来取代农药的使用。关键词:精油配方植物螨选择性荨麻叶螨毒性我们衷心感谢NRC害虫与植物保护部技术员Khaled A. Dawoud先生在实验室准备方面的宝贵帮助。声明作者声明不存在利益冲突。本研究由国家研究中心(NRC)项目No. 13050117资助。
{"title":"How could the bioactivity of emulsifiable concentrate and nano-emulsion formulations of <i>Rheum palmatum</i> and <i>Juniperus virginiana</i> essential oils affect <i>Tetranychus urticae</i> (Acari: Tetranychidae) and its bio-agent, <i>Phytoseiulus persimilis</i> (Acari: Phytoseiidae)","authors":"Marwa E. Barghout, Elham A. Sammour","doi":"10.1080/03235408.2023.2254675","DOIUrl":"https://doi.org/10.1080/03235408.2023.2254675","url":null,"abstract":"AbstractTwo types of formulations, emulsifiable concentrates (ECs) and nano-emulsions (NEs), were conducted to enhance the activity of the Rheum palmatum L. and Juniperus virginiana L. essential oils (EOs). The efficacy of EOs, ECs, and NEs was evaluated under laboratory and semi-field conditions against Tetranychus urticae Koch eggs and females. In addition, assessing their selectivity towards the predatory mite, Phytoseiulus persimilis Athias-Henriot. The tested ECs and NEs showed toxicity against T. urticae females higher than bulk oils, and all caused 100% un-hatchability of T. urticae eggs. With the exception of EOs and R. palmatum EC, neither the prepared ECs nor NEs showed any phytotoxicity symptoms on treated plants. The residual effects of tested materials were dissipated over time. Juniperus virginiana EO, EC and NE were compatible with P. persimilis. Tested ECs and NEs can aid in more sustainable crop management, to develop low-risk solutions to replace the use of pesticides.Keywords: Essential oilsformulationphytoseiid mitesselectivityTetranychus urticaetoxicity AcknowledgmentsWe are sincerely thanking Mr. Khaled A. Dawoud, technician, Pests and Plant Protection Department (NRC), for his valuable assistance in lab preparations.Disclosure statementThe authors declare there is no conflict of interest.Additional informationFundingThis work was funded by the National Research Centre (NRC) project No. 13050117.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"158 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135182996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1080/03235408.2023.2251433
Alvin Swee Ong Tang, F. Yeo, S. Chin, B. S. Wee, M. Ngu-Schwemlein
Abstract Pyricularia oryzae, the causal agent of rice blast disease, is a serious threat to rice. It reduces the yield and quality of rice. Agrofungicide used to control rice blast is harming the environment and human health. Safer alternative is needed. This study aimed to investigate the antifungal activity of zinc oxide nanoparticles (ZnO NPs) against P. oryzae of Sarawak, and its phytotoxicity towards Sarawak rice. Solvothermal sysnthesis method was used to synthesize two sizes of ZnO NPs (∼20 nm and ∼40 nm). ZnO NPs showed inhibition effect on P. oryzae tested and the effect was isolate dependent. The effect increased along with the concentration increment of ZnO NPs. ZnO NPs were found not affecting the seed germination rate, but on the shoot and root development. The effect varies at different concentration treatments of ZnO NPs. Landrace/genotype dependent phytotoxicity effect of ZnO NPs was observed.
{"title":"Antifungal properties and phytotoxicity of ZnO nanoparticles – a genotypic dependent effect","authors":"Alvin Swee Ong Tang, F. Yeo, S. Chin, B. S. Wee, M. Ngu-Schwemlein","doi":"10.1080/03235408.2023.2251433","DOIUrl":"https://doi.org/10.1080/03235408.2023.2251433","url":null,"abstract":"Abstract Pyricularia oryzae, the causal agent of rice blast disease, is a serious threat to rice. It reduces the yield and quality of rice. Agrofungicide used to control rice blast is harming the environment and human health. Safer alternative is needed. This study aimed to investigate the antifungal activity of zinc oxide nanoparticles (ZnO NPs) against P. oryzae of Sarawak, and its phytotoxicity towards Sarawak rice. Solvothermal sysnthesis method was used to synthesize two sizes of ZnO NPs (∼20 nm and ∼40 nm). ZnO NPs showed inhibition effect on P. oryzae tested and the effect was isolate dependent. The effect increased along with the concentration increment of ZnO NPs. ZnO NPs were found not affecting the seed germination rate, but on the shoot and root development. The effect varies at different concentration treatments of ZnO NPs. Landrace/genotype dependent phytotoxicity effect of ZnO NPs was observed.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"971 - 990"},"PeriodicalIF":1.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41747628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1080/03235408.2023.2251789
A. Nair, Sumna Sasidharan
Abstract The Nucleotide-binding site domain (NBD) of plant resistance (R) genes plays a vital role during plant defense signaling. The functional significance of CC-NBS-LRR (Coiled coil-NBS-Leucine Rich Repeat) class of R gene designated ZzR1, characterized from Zingiber zerumbet in earlier studies, was determined by molecular modeling and docking studies. Docked complex showed the ligand GTP interacts with amino acid residues in the cleft made by GLPL and P-loop motifs of the NBD. Heterologous expression of ZzR1 NBS protein was optimized using expression vectors, pEcoli-Nterm-6xHN and pET Directional TOPO and transformed in five Escherichia coli strains namely DH5α, TOP10, BL21DE3, BL21DE3 star and BL21plysS cells. The NBS protein of 36 kDa molecular size was expressed in E. coli BL21DE3 strain using pET TOPO vector. Optimum induction was detected at 30 °C using isopropyl-1-thio-β-D-galactopyranoside (IPTG) (1 mM). The present study provides valuable information on ligand interactions and heterologous expression of ZzR1 NBD protein. graphical ABSTRACT
{"title":"Characterizing nucleotide binding site domain (NBD) of ZzR1 resistance gene from Zingiber zerumbet: in silico ligand docking and optimizing heterologous expression","authors":"A. Nair, Sumna Sasidharan","doi":"10.1080/03235408.2023.2251789","DOIUrl":"https://doi.org/10.1080/03235408.2023.2251789","url":null,"abstract":"Abstract The Nucleotide-binding site domain (NBD) of plant resistance (R) genes plays a vital role during plant defense signaling. The functional significance of CC-NBS-LRR (Coiled coil-NBS-Leucine Rich Repeat) class of R gene designated ZzR1, characterized from Zingiber zerumbet in earlier studies, was determined by molecular modeling and docking studies. Docked complex showed the ligand GTP interacts with amino acid residues in the cleft made by GLPL and P-loop motifs of the NBD. Heterologous expression of ZzR1 NBS protein was optimized using expression vectors, pEcoli-Nterm-6xHN and pET Directional TOPO and transformed in five Escherichia coli strains namely DH5α, TOP10, BL21DE3, BL21DE3 star and BL21plysS cells. The NBS protein of 36 kDa molecular size was expressed in E. coli BL21DE3 strain using pET TOPO vector. Optimum induction was detected at 30 °C using isopropyl-1-thio-β-D-galactopyranoside (IPTG) (1 mM). The present study provides valuable information on ligand interactions and heterologous expression of ZzR1 NBD protein. graphical ABSTRACT","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"991 - 1002"},"PeriodicalIF":1.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45946100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1080/03235408.2023.2253566
Ajinkya Terkar, Vaishnavi Chavan, Mahesh Borde
The investigation aimed to isolate, identify, characterized and evaluate fungal endophytes from Embelia ribes. The main objective of the study was to identify and evaluate the fungal endophytes for PGP and antifungal activities against selected phytopathogens. A total of eight fungal endophytes were identified by amplifying the ITS region, with phylogenetic analysis confirming their placement within the Ascomycota. Phomopsis and Daldinia sp. dominant species in E. ribes. To evaluate their antifungal activities, three selected endophytes were tested against three fungal pathogens selected based on their relevance and prevalence as plant pathogens in the study area. SEM analysis of the interaction zones between the endophytes and pathogens provided insights into the inhibitory mechanism’s endophytes. Furthermore, the IAA and chitinase activities by endophytes known to exhibit inhibitory activities against pathogenic fungi. Further research is needed to understand the specific mechanisms underlying their inhibitory effects and to assess their performance under field conditions.
{"title":"Fungal endophytes associated with <i>Embelia ribes</i> and their antifungal and plant growth promoting activity against plant pathogens","authors":"Ajinkya Terkar, Vaishnavi Chavan, Mahesh Borde","doi":"10.1080/03235408.2023.2253566","DOIUrl":"https://doi.org/10.1080/03235408.2023.2253566","url":null,"abstract":"The investigation aimed to isolate, identify, characterized and evaluate fungal endophytes from Embelia ribes. The main objective of the study was to identify and evaluate the fungal endophytes for PGP and antifungal activities against selected phytopathogens. A total of eight fungal endophytes were identified by amplifying the ITS region, with phylogenetic analysis confirming their placement within the Ascomycota. Phomopsis and Daldinia sp. dominant species in E. ribes. To evaluate their antifungal activities, three selected endophytes were tested against three fungal pathogens selected based on their relevance and prevalence as plant pathogens in the study area. SEM analysis of the interaction zones between the endophytes and pathogens provided insights into the inhibitory mechanism’s endophytes. Furthermore, the IAA and chitinase activities by endophytes known to exhibit inhibitory activities against pathogenic fungi. Further research is needed to understand the specific mechanisms underlying their inhibitory effects and to assess their performance under field conditions.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1080/03235408.2023.2251907
R. López-Cruz, Guillem Segarra, R. Torres, N. Teixidó, J. A. Ragazzo‐Sánchez, M. Calderón‐Santoyo
Abstract This work aimed to broaden the spectrum biocontrol activity of Meyerozyma guilliermondii LMA-Cp01. Inhibition of postharvest pathogens, viz., Alternaria alternata, Penicillium italicum, P. digitatum, P. expansum, Botrytis cinerea, and Rhizopus stolonifer was tested on persimmon, oranges, apples, and pears. The effect of spraying or dipping application of the actinomycete for the phytopathogens biocontrol was evaluated. LMA-Cp01 reduced 30 and 50% (R. stolonifer), 53 and 67% (B. cinerea), and 55 and 67% (P. expansum) the disease incidence and severity on apples, respectively. Regarding pears, reductions of 44 and 71% (R. stolonifer) and 65 and 85% (P. expansum) of incidence and severity were respectively achieved. P. italicum and P. digitatum incidence on oranges was reduced by 70 and 72%, respectively, and the severity was also reduced by 85%. The LMA-Cp01 efficacy on apples and oranges was higher by spraying application. LMA-Cp01 is a promising broad-spectrum biocontrol agent against diseases on different hosts.
{"title":"Biocontrol efficacy of Meyerozyma guilliermondii LMA-Cp01 against post-harvest pathogens of fruits","authors":"R. López-Cruz, Guillem Segarra, R. Torres, N. Teixidó, J. A. Ragazzo‐Sánchez, M. Calderón‐Santoyo","doi":"10.1080/03235408.2023.2251907","DOIUrl":"https://doi.org/10.1080/03235408.2023.2251907","url":null,"abstract":"Abstract This work aimed to broaden the spectrum biocontrol activity of Meyerozyma guilliermondii LMA-Cp01. Inhibition of postharvest pathogens, viz., Alternaria alternata, Penicillium italicum, P. digitatum, P. expansum, Botrytis cinerea, and Rhizopus stolonifer was tested on persimmon, oranges, apples, and pears. The effect of spraying or dipping application of the actinomycete for the phytopathogens biocontrol was evaluated. LMA-Cp01 reduced 30 and 50% (R. stolonifer), 53 and 67% (B. cinerea), and 55 and 67% (P. expansum) the disease incidence and severity on apples, respectively. Regarding pears, reductions of 44 and 71% (R. stolonifer) and 65 and 85% (P. expansum) of incidence and severity were respectively achieved. P. italicum and P. digitatum incidence on oranges was reduced by 70 and 72%, respectively, and the severity was also reduced by 85%. The LMA-Cp01 efficacy on apples and oranges was higher by spraying application. LMA-Cp01 is a promising broad-spectrum biocontrol agent against diseases on different hosts.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"1003 - 1020"},"PeriodicalIF":1.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46468964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-21DOI: 10.1080/03235408.2023.2235730
H. Mahmud, M. S. Rahman, Mst. Fahliza Akter, Umma Umara, I. Hossain
Abstract The present study was conducted to evaluate the false smut incidence and its effect on yield in different varieties, and to determine the financial cost, return and profitability of rice production during the year 2018–2019 in Kushtia district. The highest average farmers (65%) lost their yield 0.85 t/ha in 5 severity scale of BRRI dhan49, while Dhani Gold as hybrid variety exerted the lowest yield losses of 0.20 t/ha in 7% affected farmers. Spraying of Nativo (75 WG) 0.40 kg/ha increased the yield 34.87% over control, and Amister Top 325 SC was applied in the field that produced lower yield compared to Nativo. Tilt (250 EC) 0.45 l/ha and Folicur 0.90 l/ha also showed significant increase of yield over untreated control plot. The highest gross return of BRRI dhan39 was determined at Tk. 114753/ha with higher price and its fine quality. The lowest gross return and net return were estimated at Tk. 106958/ha and (−) Tk. 1215/ha, respectively, in Shorna cultivar due to higher incidence of false smut disease.
{"title":"Study on financial assessment of rice production causing false smut disease of different varieties at farm level in Kushtia district","authors":"H. Mahmud, M. S. Rahman, Mst. Fahliza Akter, Umma Umara, I. Hossain","doi":"10.1080/03235408.2023.2235730","DOIUrl":"https://doi.org/10.1080/03235408.2023.2235730","url":null,"abstract":"Abstract The present study was conducted to evaluate the false smut incidence and its effect on yield in different varieties, and to determine the financial cost, return and profitability of rice production during the year 2018–2019 in Kushtia district. The highest average farmers (65%) lost their yield 0.85 t/ha in 5 severity scale of BRRI dhan49, while Dhani Gold as hybrid variety exerted the lowest yield losses of 0.20 t/ha in 7% affected farmers. Spraying of Nativo (75 WG) 0.40 kg/ha increased the yield 34.87% over control, and Amister Top 325 SC was applied in the field that produced lower yield compared to Nativo. Tilt (250 EC) 0.45 l/ha and Folicur 0.90 l/ha also showed significant increase of yield over untreated control plot. The highest gross return of BRRI dhan39 was determined at Tk. 114753/ha with higher price and its fine quality. The lowest gross return and net return were estimated at Tk. 106958/ha and (−) Tk. 1215/ha, respectively, in Shorna cultivar due to higher incidence of false smut disease.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"919 - 941"},"PeriodicalIF":1.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42037344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-21DOI: 10.1080/03235408.2023.2242078
Pooja, U. Bhardwaj, Ramandeep Kaur, Rupeet Gill
Abstract The present study aims to develop eco-friendly Emulsifiable Concentrates (EC) using citronella and lemon grass essential oil (EO) and evaluate its antifungal activity against Phomopsis vexans in Solanum melongena. Among nine prepared formulations, F1 formulation with EO (40%) + vegetable oil (50%) and emulsifier blends (10%) with hydrophilic-lipophilic balance value (12.86) were found suitable for both EO formulations. These formulations (CF1 and LF1) performed well in terms of emulsion stability, cold test, accelerated storage and foam test indicating feasibility for their commercial production. In laboratory studies, at 0.1% concentration, citronella essential oil, lemon grass essential oil and their EC formulations CF1 and LF1 inhibited the growth of P. vexans with EC50 values 0.0087, 0.0098, 0.009 and 0.0126, respectively. Further, under field conditions, CF1 performed better than LF1 formulation in terms of minimum disease severity (32.22%). Hence, EC formulations can be used as natural and eco-friendly fungicide for P. vexans control.
{"title":"Antifungal activity of essential oil and emulsifiable concentrates of citronella and lemon grass against Phomopsis vexans","authors":"Pooja, U. Bhardwaj, Ramandeep Kaur, Rupeet Gill","doi":"10.1080/03235408.2023.2242078","DOIUrl":"https://doi.org/10.1080/03235408.2023.2242078","url":null,"abstract":"Abstract The present study aims to develop eco-friendly Emulsifiable Concentrates (EC) using citronella and lemon grass essential oil (EO) and evaluate its antifungal activity against Phomopsis vexans in Solanum melongena. Among nine prepared formulations, F1 formulation with EO (40%) + vegetable oil (50%) and emulsifier blends (10%) with hydrophilic-lipophilic balance value (12.86) were found suitable for both EO formulations. These formulations (CF1 and LF1) performed well in terms of emulsion stability, cold test, accelerated storage and foam test indicating feasibility for their commercial production. In laboratory studies, at 0.1% concentration, citronella essential oil, lemon grass essential oil and their EC formulations CF1 and LF1 inhibited the growth of P. vexans with EC50 values 0.0087, 0.0098, 0.009 and 0.0126, respectively. Further, under field conditions, CF1 performed better than LF1 formulation in terms of minimum disease severity (32.22%). Hence, EC formulations can be used as natural and eco-friendly fungicide for P. vexans control.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"942 - 960"},"PeriodicalIF":1.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48461729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-21DOI: 10.1080/03235408.2023.2238874
A. Chávez, C. C. Cazal-Martínez, M. Kohli, M. Carmona
Abstract Given the scarcity of information about inoculum sources of Pyricularia oryzae pathotype Triticum (PoT) under field conditions, this study quantified the conidia of PoT on wheat leaves during the crop cycle and linked them with the presence of blast in the spikes. The study was conducted involving two different production environments: nine commercial wheat fields and an experimental plot under sprinkler irrigation. Throughout the crop season, leaves were collected monthly and processed to quantify the number of conidia present in each gram of dry leaves. At physiological maturity of the crop, the fields were sampled to quantify the number of infected spikes/m2. Significant differences in the quantity and persistence of the pathogen during the cycle were seen under both environments. A strong positive correlation between the number of conidia present on the leaves during the crop growth cycle and the percentage of damaged spikes was established.
{"title":"Relationship between the conidia production of Pyricularia oryzae on wheat leaves and the blast disease development in wheat spikes","authors":"A. Chávez, C. C. Cazal-Martínez, M. Kohli, M. Carmona","doi":"10.1080/03235408.2023.2238874","DOIUrl":"https://doi.org/10.1080/03235408.2023.2238874","url":null,"abstract":"Abstract Given the scarcity of information about inoculum sources of Pyricularia oryzae pathotype Triticum (PoT) under field conditions, this study quantified the conidia of PoT on wheat leaves during the crop cycle and linked them with the presence of blast in the spikes. The study was conducted involving two different production environments: nine commercial wheat fields and an experimental plot under sprinkler irrigation. Throughout the crop season, leaves were collected monthly and processed to quantify the number of conidia present in each gram of dry leaves. At physiological maturity of the crop, the fields were sampled to quantify the number of infected spikes/m2. Significant differences in the quantity and persistence of the pathogen during the cycle were seen under both environments. A strong positive correlation between the number of conidia present on the leaves during the crop growth cycle and the percentage of damaged spikes was established.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"961 - 968"},"PeriodicalIF":1.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47617098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1080/03235408.2023.2238875
N. C. Hieu, H. T. Thao, Dao Thi Dieu Linh, L. K. Oanh, Phạm Thị Thu Huyền, Hoàng Kim Diệu, T. Ha, Vu Hong Nhu Yen, T. Hong, Ngo Quang Huy, Phạm Hồng Hiển, D. Nguyen
Abstract The most significant issue affecting balloon flower (Platycodon grandiflorus) in Vietnam’s mountainous provinces is stem and root rot disease. The purpose of this study was to identify the causal fungus Fusarium solani of balloon flower planted in Lao Cai province. F. solani’s identity was confirmed through DNA sequencing and phylogenetic analyses based on ITS sequences. PDA was the most suitable medium for F. solani growth and development of the three semi-solid media tested (CA, PDA, and CDA). The optimal temperature and pH conditions for its growth ranged from 25-30 °C with a maximum growth rate of 25 °C and pH 5.5-7.0 with a maximum growth rate of 6.5. This is the first report of F. solani causing balloon flower stem and root rot in Vietnam.
{"title":"Molecular characterization of Fusarium solani causing stem and root rot disease of balloon flower (Platycodon grandiflorus) in Viet Nam","authors":"N. C. Hieu, H. T. Thao, Dao Thi Dieu Linh, L. K. Oanh, Phạm Thị Thu Huyền, Hoàng Kim Diệu, T. Ha, Vu Hong Nhu Yen, T. Hong, Ngo Quang Huy, Phạm Hồng Hiển, D. Nguyen","doi":"10.1080/03235408.2023.2238875","DOIUrl":"https://doi.org/10.1080/03235408.2023.2238875","url":null,"abstract":"Abstract The most significant issue affecting balloon flower (Platycodon grandiflorus) in Vietnam’s mountainous provinces is stem and root rot disease. The purpose of this study was to identify the causal fungus Fusarium solani of balloon flower planted in Lao Cai province. F. solani’s identity was confirmed through DNA sequencing and phylogenetic analyses based on ITS sequences. PDA was the most suitable medium for F. solani growth and development of the three semi-solid media tested (CA, PDA, and CDA). The optimal temperature and pH conditions for its growth ranged from 25-30 °C with a maximum growth rate of 25 °C and pH 5.5-7.0 with a maximum growth rate of 6.5. This is the first report of F. solani causing balloon flower stem and root rot in Vietnam.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"903 - 917"},"PeriodicalIF":1.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48151571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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