The composition and distribution of macro-invertebrates of benthic species can be influenced by water quality. The primary objective of this study was to understand the spatial distribution and composition of macro-invertebrates of benthic nature in the upper Awash River below and above Ginchi Town. The study was carried out for a period of three months from February 2020 to April 2020, and macro-invertebrates samples were collected from three stations using Surber sampler with 500 (upmu)m/mesh size and 25 cm × 25 cm (0.0625 m2) of sampling area coverage. The present study showed that, in the study area, there was a low density of macro-invertebrates and species richness in general with a comparative difference between the pool riffle habitats in density and family richness. And the total abundance of benthic macro-invertebrates showed a positive correlation with NO3-N, dissolved oxygen, and total phosphorus, while a negative correlation with all the other physicochemical parameters measured during the study period. Spatial and monthly variations in the diversity and richness of benthic macro-invertebrates were attributed to the effects of human action and changes in environmental factors. So, the water shade management system should be applied to control pollution factors that can affect the macro-benthic fauna community.