Archives of Environmental Contamination and Toxicology最新文献

This study analyzed microplastics (MPs; target size: 0.1–5 mm) in freshwater fish and aquatic plants in an urban lake in Kumamoto, Japan. MPs were detected in 82% of the collected sample of herbivorous fish species, Nile tilapia (Oreochromis niloticus) and Redbelly tilapia (Tilapia zillii) at mean level of 10 ± 13 items ind⁻¹, which was more than 10 times higher than the levels found in two carnivorous fish species, largemouth bass (Micropterus salmoides) and snakehead (Channa argus). Fish samples near the outlets of stormwater runoff and a wastewater treatment plant showed higher abundances of MPs than fish collected from other locations in the lake. Polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET) were dominant polymers in fish, accounting for 83%. These three polymers were also commonly detected in three species of aquatic plants, which retained MPs at 4.6–35 items g⁻¹ dry weight. A comparison of the characteristics of the MPs detected in the herbivorous fish and aquatic plant samples collected at the same station showed similar profiles in terms of polymer types, shape, and colors. This indicates that herbivorous fish are exposed to MPs adsorbed onto the surface of aquatic plants through their feeding activity. Our findings highlighted that aquatic plants act as a mediator to increase the bioavailability of MPs in freshwater fish by trapping MPs on the plant surface.

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This study aimed to comprehensively analyze the accumulation and distribution patterns of polycyclic aromatic hydrocarbons (PAHs) in plankton across varying exposure durations within a simulated planktonic food chain, consisting of Tetraselmis chuii (phytoplankton), Brachionus rotundiformis (rotifers), and Apocyclops sp. (copepods) cultured in a gas purging system with a continuous supply of PAHs. The investigation revealed three distinct temporal phases of PAH accumulation: before 1 h, 1–24 h, and 24–120 h. Notably, PAH concentrations exhibited substantial fluctuations during the initial two intervals but approached a steady state after 24 h of exposure. An intriguing observation was the differential accumulation of high solubility (> 1) PAHs, which were found in higher quantities in copepods compared to rotifers and phytoplankton. Conversely, low solubility (< 1) PAHs were significantly more abundant in phytoplankton, indicating potential selectivity in PAH uptake among planktonic organisms. Principal component analysis (PCA) elucidated a clear demarcation between phytoplankton and zooplankton, suggesting distinct PAH accumulation patterns influenced by trophic levels. Linear relationship was found between the bioconcentration factor (BCF) and PAH hydrophobicity (K_ow) for all PAHs within the planktonic food chain. The varying slopes in the linear regression between log BCF and log K_ow for phytoplankton, rotifers, and copepods underscored the existence of diverse pathways for PAH accumulation among planktonic organisms. This study offers valuable insights into the complex dynamics of PAH bioaccumulation, with potential implications for understanding the broader ecological impacts of PAHs.

Studies on temporal variation of metal concentrations and their relationship with metal-binding proteins in crocodilians remain scarce. This study aimed to assess interannual changes in physiological (Cu and Zn) and xenobiotic (Hg and Cd) metals, as well as metallothioneins (MTs) levels in blood plasma of 17 semicaptive Morelet’s crocodiles (Crocodylus moreletii) sampled twice (n = 34 samples) in 2016 and 2018. Metal concentrations were quantified in plasma samples, and MTs were measured as biomarkers of metal exposure. Results showed a 1.4-fold increase in Hg and 3.5-fold increase in Cd concentrations over time, possibly reflecting dietary exposure or other unmonitored environmental factors. In contrast, Cu concentrations decreased, while Zn remained stable. MTs levels did not differ significantly between years; however, linear models suggested associations between MTs and both Cu and Cd, highlighting their potential role in homeostasis and detoxification. Although based on a small sample size and limited to plasma, these findings offer insights into metal dynamics under semicaptive conditions and support the value of MTs as candidate biomarkers for future monitoring. Improved temporal frameworks and direct environmental or dietary data are needed to better interpret these trends and support conservation strategies for this endangered species.

Gadolinium (Gd) and Lanthanum (La) are rare earth elements (REEs) widely employed in contemporary technologies due to their unique physicochemical properties. However, their increasing release into aquatic environments has raised concerns about potential ecotoxicological effects. This study assessed the acute biochemical and molecular response of fourth-instar Chironomus sancticaroli larvae exposed to Gd and La at 0.03, 0.30, 3.00, and 30 μg L⁻¹. Exposure to La at 0.03 and 3.00 μg L⁻¹ induced a significant increase in acetylcholinesterase (AChE) activity, suggesting potential neurotoxic effects that may alter synaptic transmission and behavioral responses in larvae, whereas Gd did not affect AChE activity. Furthermore, La inhibited esterase-α activity, while Gd inhibited esterase-β activity in larvae exposed to 0.30, 3.00, and 30 μg L⁻¹, suggesting specific alterations in detoxification and metabolic processing pathways. Both REEs triggered oxidative stress, as evidenced by the increase in catalase and glutathione S-transferase activities at specific concentrations, which may reflect a compensatory response to increased reactive oxygen species. Lipid peroxidation was detected only at the highest La concentration (30 μg L⁻¹), indicating membrane damage and potential impairment of cellular integrity. Gene expression analysis showed downregulation of hemoglobin D across all La concentrations and hemoglobin E across all Gd concentrations, potentially compromising oxygen transport and respiratory efficiency of larvae. These sublethal alterations suggested that C. sancticaroli larvae are sensitive to both elements, with La exerting broader physiological disruption, in addition, these findings rise concern about the ecological risk of REE contamination in freshwater ecosystems.

This study provides comprehensive information on the abundance of microplastics (MPs) in wastewater effluents, sand, and seawater samples collected from areas in Northern Greece. Sampling was conducted over a one-year period to account for seasonal variations in MP abundance. The collected samples were analyzed using microscopic and spectroscopic techniques, yielding valuable insights into the physicochemical characteristics of MPs. MPs concentrations in sand samples ranged from 15.5 ± 6.2 to 174.7 ± 17.3 items kg⁻¹ of dry sand, while the highest concentrations were recorded in winter. Among aqueous matrices, the highest MP concentrations were found in wastewater effluents, ranging from 0.43 ± 0.17 to 1.72 ± 0.45 items L⁻¹, with greater loads detected during wet seasons. In seawater samples, MP abundance ranged from 0.078 ± 0.011 to 0.989 ± 0.35 items m⁻³, with the highest concentrations recorded during the summer season. Regarding polymer identification, the analysis of MPs was conducted using micro-FTIR instrumentation, and the most frequently detected polymers in the examined samples were PE (poly(ethylene), PP (polypropylene), PET (poly(ethylene terephthalate)), and PA (polyamide). Morphological analysis revealed that fibers were the dominant MP shape across all matrices. Size distribution analysis indicated that most MPs particles were small-sized (< 1 mm). The morphological and chemical characteristics of MPs indicate strong links among matrices. SEM-EDX analysis revealed metals on MPs from sand samples, reinforcing these connections. This study investigates MP abundance across environments, uncovering their sources, inter-matrix relationships, and interactions with pollutants, offering insights into patterns of environmental contamination.

This study introduces a non-invasive method for monitoring environmental pollution by analysing metal(loid)s in the faeces of sentinel species. We measured the concentrations of four metal(loid)s, arsenic (As), chromium (Cr), cadmium (Cd), and lead (Pb), in the droppings of four large wading bird species (Little Egret, Great Egret, Reef Heron, and Striated Heron) from 2020 to 2024 in the wetland ecosystem of the Eastern Province of Saudi Arabia, a part of the Central Asian Flyway. Our results revealed significant level of the metal(loid)s in the bird droppings, indicating environmental pollution likely linked to industrial activities, agricultural runoff, and urban expansion. All metal(loid)s exhibited significant temporal variation, with increasing concentrations observed across species. Pb and Cd also showed rising trends but exhibited species-specific effects. While the metal(loid)s analysis confirms exposure and accumulation, it does not by itself allow definitive identification of pollutant sources. Based on previous environmental assessments and land-use data, the studied wetlands are known to be influenced by anthropogenic activities, suggesting that the metal(loid)s in bird faeces predominantly originate from local pollution. However, we acknowledge that further source-tracing analyses would strengthen this inference. This study highlights the need for continuous monitoring of metal(loid)s pollution to safeguard wildlife health and maintain ecological stability. Given the study area’s proximity to industrial zones, these findings advocate for enhanced pollution control measures and targeted management strategies to mitigate risks and conserve wetland habitats.

Invasive aquatic plants threaten the health of aquatic ecosystems, and demand for chemical control is likely to increase as nuisance levels are reached. Diquat is a contact herbicide registered in many countries to control invasive aquatic plants. The objective of our study was to assess the effects of the aquatic herbicide diquat (Reward®) on North American native and non-native plants, algal communities, an amphipod and an amphibian using outdoor mesocosms to simulate natural systems. Our experimental design included a control and five nominal concentrations of diquat ranging from 100% (18.3 L/ha; 1153 µg/L) to 6.4% (1.2 L/ha; 74 µg/L) of the label rate of a single diquat application. Effects of diquat were found to vary among study organisms. All four plant species were negatively affected at all concentrations, exhibiting either mortality or severe reductions in dry biomass (< 1% the biomass of the controls). In contrast, phytoplankton biomass increased 7 d following diquat application concomitant with significant changes in algal community structure. A concentration–response relationship was observed for amphipod survival (LC₅₀ at 6 weeks = 155 µg/L) with 100% mortality in the highest treatment after two weeks. In contrast, diquat had a significant positive effect on tadpole survival, growth and development, possibly because of the higher algal biomass and decaying plant tissues. A lower label rate than currently recommended, at least in waterbodies with low turbidity, could provide effective control of target species while reducing effects on non-target biota.

Microplastics have been found in every part of the planet and in almost every tissue type that has been tested. They are a complex class of pollutants with multiple direct and indirect effects. There is an urgent and growing need to understand their toxicological impacts. Here we measured the effects of two size classes (13.2 µm ± 8.1 and 595.7 µm ± 187.3) of aged polyethylene microplastics, in the presence or absence of copper (10 μg/L), on the behavior of zebrafish and on their ability to respond to a conspecific alarm cue. Additionally, we measured the effects of the microplastics and copper on metal bioaccumulation and induction of metallothionein. Exposure to microplastics decreased the swimming speed of the zebrafish but may have reduced some of the effects of copper. Fish exposed to copper and microplastics had higher swimming velocities than fish exposed to copper alone. However, large microplastics also increased copper bioaccumulation and metallothionein production. Fish that were not exposed to copper decreased their swimming speed after addition of the alarm cue, but there was also an interaction with microplastics. Multivariate analysis of swimming behavior showed that fish exposed to small microplastics and copper formed a distinctive group after addition of the alarm, suggesting that it altered their behavioral responses. Our results show complex interactions between microplastics and copper and highlight the need for comprehensive risk assessment under different environmental scenarios. Fish rely on olfaction to find food and avoid predators. It is well established that metals can inhibit olfaction in fish. Microplastics may interact with metallic pollutants to increase their impact.

Urban freshwater streams across northern latitudes are undergoing increasing salinization due, in part, to road salt inputs during winter months. Road salt contamination has been monitored across Canada for over 40 years; however, the scale of contamination in the Pacific Northwest, which experiences relatively mild and rainy winters, is not well understood. A network of almost 40 water quality loggers in the Lower Mainland of Vancouver, B.C., Canada (VLM) was leveraged to better understand the scale of road salt inputs to local streams and identify factors that influence the magnitude and occurrence of these contamination events. Specific conductance data from these loggers indicate that road salt is entering creeks, resulting in brief salt pulses that typically last 1 day or less. Road salt pulses occur as frequently as three times per week in winter months and can attain maximum chloride concentrations above British Columbia’s acute guideline for chloride (600 mg/L Cl⁻) by as much as 11-fold in streams. The amount of road salt entering creeks is influenced by the extent of impervious surface in the surrounding catchment basin, with more urbanized creeks receiving higher inputs. Interestingly, cumulative salt inputs do not correlate with winter severity and remain consistent even during mild winters. Acute pulses of road salt occur in VLM streams between November and March, coinciding with the spawning and incubation period of locally important Pacific salmon species such as coho and chum salmon. This timing poses a direct risk to developing salmonids, and the benthic invertebrates which sustain them later in development.

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Lead (Pb) poisoning poses a significant threat to wildlife. A primary cause of Pb poisoning is the unintentional ingestion of Pb ammunition and fishing weights, which are still used for hunting and fishing in numerous regions globally. While the effects of Pb poisoning on birds and mammals are well established, impacts on reptiles are less well documented and difficult to assess under field conditions. In this study, we investigated the effects of extreme Pb exposure on captive sub-adult Nile crocodiles (Crocodylus niloticus; n = 18). We administered Pb dosages in the form of fishing weights (54–215 g) and monitored changes in blood lead concentrations, packed cell volumes, urine Pb concentrations, growth, and body condition over a 48-week period. Crocodiles exhibited a remarkable tolerance to exceptionally high Pb exposure over the duration of the study. Despite the lack of obvious clinical signs of Pb toxicity, elevated BPb concentrations were linked to lower PCVs, indicating anaemia across all treatment groups by week eight. However, crocodiles showed a sustained erythropoietic response which may be contributing to their resilience to acute Pb toxicity. While Pb exposure did not significantly affect body condition, it was associated with a discernible reduction in weight gain over the duration of the study. Our estimation of a 5.8–7.3-year timeframe for complete dissolution of the Pb fishing weights in the experimental crocodiles’ stomachs carries significant implications for wild populations, which are likely to be exposed to Pb for far longer than 48-week duration of this study.