Pub Date : 2024-07-01Epub Date: 2024-06-13DOI: 10.1007/s00244-024-01069-7
Laura Gasque-Belz, Bradley Park, Steven Siciliano, Natacha Hogan, Lynn Weber, Patrick Campbell, Rachel Peters, Mark Hanson, Markus Hecker
Complex mixtures of chemicals present in groundwater at legacy-contaminated industrial sites can pose significant risks to adjacent surface waters. The combination of short-term molecular and chronic apical effect assessments is a promising approach to characterize the potential hazard of such complex mixtures. The objectives of this study were to: (1) assess the apical effects (survival, growth, development, and liver histopathology) after chronic exposure of early life stages (ELSs) of fathead minnows (FHM; Pimephales promelas) to contaminated groundwater from a legacy-contaminated pesticide manufacturing and packaging plant, and (2) identify possible molecular mechanisms responsible for these effects by comparing results to mechanistic outcomes previously determined by a short-term reduced transcriptome assay (EcoToxChips). This study revealed a significant increase in mortality and prevalence of spinal curvatures, as well as a significant reduction in the length of FHMs exposed to the groundwater mixtures in a concentration-dependent manner. There was an increasing trend in the prevalence of edema in FHMs, though not significantly different from controls. Additionally, no histopathological effects were observed in the liver of FHMs exposed to the groundwater mixtures. Short-term molecular outcomes determined in a parallel study were found to be informative of chronic apical outcomes, including cardiotoxicity, spinal deformities, and liver toxicity. Overall, the results observed in this study demonstrated that short-term transcriptomics analyses could support the hazard assessment of complex contaminated sites.
{"title":"Characterization of Adverse Outcomes from Legacy-Contaminated Groundwater Exposure to Early Life Stages of Fathead Minnow.","authors":"Laura Gasque-Belz, Bradley Park, Steven Siciliano, Natacha Hogan, Lynn Weber, Patrick Campbell, Rachel Peters, Mark Hanson, Markus Hecker","doi":"10.1007/s00244-024-01069-7","DOIUrl":"10.1007/s00244-024-01069-7","url":null,"abstract":"<p><p>Complex mixtures of chemicals present in groundwater at legacy-contaminated industrial sites can pose significant risks to adjacent surface waters. The combination of short-term molecular and chronic apical effect assessments is a promising approach to characterize the potential hazard of such complex mixtures. The objectives of this study were to: (1) assess the apical effects (survival, growth, development, and liver histopathology) after chronic exposure of early life stages (ELSs) of fathead minnows (FHM; Pimephales promelas) to contaminated groundwater from a legacy-contaminated pesticide manufacturing and packaging plant, and (2) identify possible molecular mechanisms responsible for these effects by comparing results to mechanistic outcomes previously determined by a short-term reduced transcriptome assay (EcoToxChips). This study revealed a significant increase in mortality and prevalence of spinal curvatures, as well as a significant reduction in the length of FHMs exposed to the groundwater mixtures in a concentration-dependent manner. There was an increasing trend in the prevalence of edema in FHMs, though not significantly different from controls. Additionally, no histopathological effects were observed in the liver of FHMs exposed to the groundwater mixtures. Short-term molecular outcomes determined in a parallel study were found to be informative of chronic apical outcomes, including cardiotoxicity, spinal deformities, and liver toxicity. Overall, the results observed in this study demonstrated that short-term transcriptomics analyses could support the hazard assessment of complex contaminated sites.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-06-02DOI: 10.1007/s00244-024-01071-z
Catherine Estefany Davila-Arenas, Lorne Doig, Xiaowen Ji, Banamali Panigrahi, Immanuela Ezugba, Karsten Liber
Significant amounts of tailings and oil sands process-affected water (OSPW) are generated by bitumen extraction in the Alberta Oil Sands region. These by-products are potentially toxic to aquatic organisms and require remediation. The study site was Lake Miwasin, a pilot-scale pit lake integrated into broader reclamation efforts. It consists of treated tailings overlaid with blended OSPW and freshwater, exhibiting meromictic conditions and harboring aquatic communities. This study assessed the potential toxicity of Lake Miwasin surface water (LMW) and pore water (LMP) using saline-acclimated Cladocera, including lab strains of Daphnia magna and Daphnia pulex and native Daphnia species collected in brackish Humboldt Lake (HL) and Lake Miwasin (LM). The pore water evaluation was used to represent a worst-case water quality scenario during pond stratification. Additionally, the inclusion of native organisms incorporated site-specific adaptations and regional sensitivity into the toxicity evaluation. Our results showed that LMW did not display acute or chronic toxicity to lab species and native Daphnia sp. (HL). Conversely, LMP was acutely toxic to both lab species and native D. pulex (LM). In chronic tests (12 days exposure), LMP negatively affected reproduction in D. pulex (lab), with reductions in the number of offspring. Limited ability to acclimated organisms to the high salinity levels of LMP resulted in a shortened exposure duration for the chronic toxicity test. In addition to salinity being identified as a stressor in LMP, toxicity identification evaluation (TIE) phase I findings demonstrated that the observed toxicity for D. magna (lab) and D. pulex (LM, native) might be attributed to ammonia and metals in LMP. Further investigations are required to confirm the contributions of these stressors to LMP toxicity.
{"title":"Toxicity Evaluation of Water and Pore Water from a Pilot-Scale Pit Lake in the Alberta Oil Sands Region to Daphnia Species.","authors":"Catherine Estefany Davila-Arenas, Lorne Doig, Xiaowen Ji, Banamali Panigrahi, Immanuela Ezugba, Karsten Liber","doi":"10.1007/s00244-024-01071-z","DOIUrl":"10.1007/s00244-024-01071-z","url":null,"abstract":"<p><p>Significant amounts of tailings and oil sands process-affected water (OSPW) are generated by bitumen extraction in the Alberta Oil Sands region. These by-products are potentially toxic to aquatic organisms and require remediation. The study site was Lake Miwasin, a pilot-scale pit lake integrated into broader reclamation efforts. It consists of treated tailings overlaid with blended OSPW and freshwater, exhibiting meromictic conditions and harboring aquatic communities. This study assessed the potential toxicity of Lake Miwasin surface water (LMW) and pore water (LMP) using saline-acclimated Cladocera, including lab strains of Daphnia magna and Daphnia pulex and native Daphnia species collected in brackish Humboldt Lake (HL) and Lake Miwasin (LM). The pore water evaluation was used to represent a worst-case water quality scenario during pond stratification. Additionally, the inclusion of native organisms incorporated site-specific adaptations and regional sensitivity into the toxicity evaluation. Our results showed that LMW did not display acute or chronic toxicity to lab species and native Daphnia sp. (HL). Conversely, LMP was acutely toxic to both lab species and native D. pulex (LM). In chronic tests (12 days exposure), LMP negatively affected reproduction in D. pulex (lab), with reductions in the number of offspring. Limited ability to acclimated organisms to the high salinity levels of LMP resulted in a shortened exposure duration for the chronic toxicity test. In addition to salinity being identified as a stressor in LMP, toxicity identification evaluation (TIE) phase I findings demonstrated that the observed toxicity for D. magna (lab) and D. pulex (LM, native) might be attributed to ammonia and metals in LMP. Further investigations are required to confirm the contributions of these stressors to LMP toxicity.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-06-19DOI: 10.1007/s00244-024-01073-x
Sebastiano La Maestra, Mirko Benvenuti, Stefano Alberti, Linda Ferrea, Francesco D'Agostini
Plastics are synthetic organic compounds whose widespread use generates enormous waste. Different processes, such as mechanical abrasion, microbiological activity, and UVB irradiation, can fragment the plastic material and generate microplastics (MPs). MPs are ubiquitous, and various organisms, including humans, can ingest or inhale them, with potential adverse health effects. The differences between UV-aged and virgin particles were studied to evaluate the genotoxic damage and oxidative stress induced by polystyrene MPs with 1 and 5 µm sizes on the monocyte-like cell line (THP-1). Fourier transform infrared spectroscopy and Ζ-potential measurements were used to characterise MP particles after UVB exposure. Cells exposed to MPs show a widespread change in the cellular environment with the generation of reactive oxidative species (ROS), as indicated by the increased malondialdehyde level. The occurrence of genotoxic damage is correlated to the smaller size and ageing state of the MPs. The biochemical and genomic alterations observed in this in vitro study suggest that MPs, ubiquitous pollutants, following natural degradation and oxidation processes can cause various adverse effects on the health of the exposed population, making it necessary to carry out further studies to better define the real risk.
{"title":"UVB-Aged Microplastics and Cellular Damage: An in Vitro Study.","authors":"Sebastiano La Maestra, Mirko Benvenuti, Stefano Alberti, Linda Ferrea, Francesco D'Agostini","doi":"10.1007/s00244-024-01073-x","DOIUrl":"10.1007/s00244-024-01073-x","url":null,"abstract":"<p><p>Plastics are synthetic organic compounds whose widespread use generates enormous waste. Different processes, such as mechanical abrasion, microbiological activity, and UVB irradiation, can fragment the plastic material and generate microplastics (MPs). MPs are ubiquitous, and various organisms, including humans, can ingest or inhale them, with potential adverse health effects. The differences between UV-aged and virgin particles were studied to evaluate the genotoxic damage and oxidative stress induced by polystyrene MPs with 1 and 5 µm sizes on the monocyte-like cell line (THP-1). Fourier transform infrared spectroscopy and Ζ-potential measurements were used to characterise MP particles after UVB exposure. Cells exposed to MPs show a widespread change in the cellular environment with the generation of reactive oxidative species (ROS), as indicated by the increased malondialdehyde level. The occurrence of genotoxic damage is correlated to the smaller size and ageing state of the MPs. The biochemical and genomic alterations observed in this in vitro study suggest that MPs, ubiquitous pollutants, following natural degradation and oxidation processes can cause various adverse effects on the health of the exposed population, making it necessary to carry out further studies to better define the real risk.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-07-03DOI: 10.1007/s00244-024-01075-9
J W Roy, L Grapentine
While in situ toxicity testing with caged organisms has been used to assess surface water and sediment contamination, no successful application to benthic organisms exposed to highly contaminated groundwater plumes discharging to surface waters has been reported. The objective of this study was to demonstrate and evaluate this application using four sets of tests performed at three previously reported contaminated groundwater sites, which include one river site affected by volatile organic contaminant plumes, and two sites, one pond and one small urban stream, impacted by landfill plumes. The study examined multiple cage designs and orientations and two test organisms: an amphipod (Hyalella azteca) and midge larvae (Chironomus riparius; only one study). Cages were deployed for between 5 and 28 days and assessed for organism survival and growth. At all sites and for some deployment conditions, cages exposed to high contaminant concentrations in the plume footprint had greater mortality compared to those exposed to lower or background concentrations. Organism growth was less clear as a metric of toxicity. Vertically oriented cages typically showed high mortality to plume contaminants, but some were also affected by other non-target groundwater conditions (e.g., low dissolved oxygen, other contaminant sources), while horizontally oriented cages were rarely responsive to either groundwater influence. A hybrid cage design showed much promise in its single study. Useful observations on the test organisms and on potentially problematic site conditions were also made. The informed use of in situ toxicity cages could be an additional beneficial tool for groundwater contaminated site assessments.
{"title":"Insights into In Situ Benthic Caging Tests for Ecotoxicity Assessments Targeting Discharging Groundwater Contaminant Plumes.","authors":"J W Roy, L Grapentine","doi":"10.1007/s00244-024-01075-9","DOIUrl":"10.1007/s00244-024-01075-9","url":null,"abstract":"<p><p>While in situ toxicity testing with caged organisms has been used to assess surface water and sediment contamination, no successful application to benthic organisms exposed to highly contaminated groundwater plumes discharging to surface waters has been reported. The objective of this study was to demonstrate and evaluate this application using four sets of tests performed at three previously reported contaminated groundwater sites, which include one river site affected by volatile organic contaminant plumes, and two sites, one pond and one small urban stream, impacted by landfill plumes. The study examined multiple cage designs and orientations and two test organisms: an amphipod (Hyalella azteca) and midge larvae (Chironomus riparius; only one study). Cages were deployed for between 5 and 28 days and assessed for organism survival and growth. At all sites and for some deployment conditions, cages exposed to high contaminant concentrations in the plume footprint had greater mortality compared to those exposed to lower or background concentrations. Organism growth was less clear as a metric of toxicity. Vertically oriented cages typically showed high mortality to plume contaminants, but some were also affected by other non-target groundwater conditions (e.g., low dissolved oxygen, other contaminant sources), while horizontally oriented cages were rarely responsive to either groundwater influence. A hybrid cage design showed much promise in its single study. Useful observations on the test organisms and on potentially problematic site conditions were also made. The informed use of in situ toxicity cages could be an additional beneficial tool for groundwater contaminated site assessments.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-06-09DOI: 10.1007/s00244-024-01072-y
Fernando Marques Quintela, Fabiano Aguiar da Silva, Fabiano Correa, Fernando Rogério Carvalho, Daniel Galiano, Mel Christine Oliveira Pires, Ulisses Galatti
The present study investigated the levels of As, Ag, Al, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Se, and Zn in muscle samples of six fish species (Pygocentrus nattereri, Serrasalmus marginatus, Mylossoma duriventre, Leporinus friderici, Pimelodus argenteus, and P. pantaneiro) highly consumed by local riverine and non-resident people in middle Miranda River, southern Brazilian Pantanal. Significant differences were detected for Ba, Fe, Mn, and Zn levels among the analyzed species. Pairwise comparison detected significant differences in element levels between species with similar diets, which implies that other factors, instead of species' feeding habits, could be involved in distinct levels of element bioaccumulation. Significant correlations between body size and concentrations were found for Ba in P. nattereri (moderate positive correlation), and for Mn in M. duriventre (weak positive correlation) and P. argenteus (moderate negative correlation). Levels exceeding tolerable daily intake (TDI) were found for Pb maximum concentrations in P. nattereri, M. duriventre, L. friderici, P. argenteus, and P. pantaneiro, and for Cd maximum concentration in P. argenteus. Health risk assessment indicated considerable risk only for the worst-case scenario (calculated from maximum concentrations) for all species. Adopting a Pb monitoring program in biotic and abiotic compartments in Miranda River is strongly recommended.
本研究调查了巴西南部潘塔纳尔地区米兰达河中游当地居民和非居民大量食用的六种鱼类(Pygocentrus nattereri、Serrasalmus marginatus、Mylossoma duriventre、Leporinus friderici、Pimelodus argenteus 和 P. pantaneiro)肌肉样本中的砷、银、铝、钡、镉、铬、铜、铁、汞、锰、铅、硒和锌含量。在分析的物种中,钡、铁、锰和锌的含量存在显著差异。配对比较发现,食性相似的物种之间元素水平存在显著差异,这意味着造成元素生物累积水平不同的可能是其他因素,而不是物种的摄食习惯。体型与元素浓度之间存在显著的相关性:钡在 P. nattereri(中度正相关)中,锰在 M. duriventre(弱正相关)和 P. argenteus(中度负相关)中。在 P. nattereri、M. duriventre、L. friderici、P. argenteus 和 P. pantaneiro 中发现的铅最高浓度和 P. argenteus 中的镉最高浓度均超过了每日可容忍摄入量 (TDI)。健康风险评估表明,所有物种只有在最坏情况下(根据最大浓度计算)才会面临相当大的风险。强烈建议在米兰达河的生物区和非生物区开展铅监测计划。
{"title":"Essential and Non-Essential Elements Levels in Fish Species Highly Consumed in the Middle Miranda River, Brazilian Pantanal.","authors":"Fernando Marques Quintela, Fabiano Aguiar da Silva, Fabiano Correa, Fernando Rogério Carvalho, Daniel Galiano, Mel Christine Oliveira Pires, Ulisses Galatti","doi":"10.1007/s00244-024-01072-y","DOIUrl":"10.1007/s00244-024-01072-y","url":null,"abstract":"<p><p>The present study investigated the levels of As, Ag, Al, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Se, and Zn in muscle samples of six fish species (Pygocentrus nattereri, Serrasalmus marginatus, Mylossoma duriventre, Leporinus friderici, Pimelodus argenteus, and P. pantaneiro) highly consumed by local riverine and non-resident people in middle Miranda River, southern Brazilian Pantanal. Significant differences were detected for Ba, Fe, Mn, and Zn levels among the analyzed species. Pairwise comparison detected significant differences in element levels between species with similar diets, which implies that other factors, instead of species' feeding habits, could be involved in distinct levels of element bioaccumulation. Significant correlations between body size and concentrations were found for Ba in P. nattereri (moderate positive correlation), and for Mn in M. duriventre (weak positive correlation) and P. argenteus (moderate negative correlation). Levels exceeding tolerable daily intake (TDI) were found for Pb maximum concentrations in P. nattereri, M. duriventre, L. friderici, P. argenteus, and P. pantaneiro, and for Cd maximum concentration in P. argenteus. Health risk assessment indicated considerable risk only for the worst-case scenario (calculated from maximum concentrations) for all species. Adopting a Pb monitoring program in biotic and abiotic compartments in Miranda River is strongly recommended.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-06-26DOI: 10.1007/s00244-024-01074-w
Juliana Rodrigues da Costa, Mariana V Capparelli, Pedro Magalhães Padilha, Emanuelle Borges, Andressa C Ramaglia, Michelle Roberta Dos Santos, Alessandra Augusto
Environmental stressors in aquatic organisms can be assessed using a bioenergetic approach based on the evaluation of changes in their physiological parameters. We evaluated the chronic effects of cadmium (Cd2+) on the energy balance as well as the survival, growth, metabolism, nitrogen excretion, hepatosomatic index, oxidized energy substrate, and osmoregulation of the shrimp Penaeus vannamei with the hypothesis that the high energy demand related to the homeostatic regulation of Cd2+could disrupt the energy balance and as a consequence, their physiological functions. The shrimp exposed to Cd2+ had higher mortality (30%), directed more energy into growth (33% of energy intake), ingested 10% more energy, and defecated less than control animals. Cd2+ exposure caused a tendency to decrease metabolism and ammonia excretion but did not alter the hepatosomatic index, type of energy substrate oxidized, and the hyperosmorregulatory pattern of the species. The Cd+2 exposure may have induced a trade-off response because there was a growth rate increase accompanied by increased mortality.
{"title":"Chronic Cadmium Exposure can Alter Energy Allocation to Physiological Functions in the Shrimp Penaeus vannamei.","authors":"Juliana Rodrigues da Costa, Mariana V Capparelli, Pedro Magalhães Padilha, Emanuelle Borges, Andressa C Ramaglia, Michelle Roberta Dos Santos, Alessandra Augusto","doi":"10.1007/s00244-024-01074-w","DOIUrl":"10.1007/s00244-024-01074-w","url":null,"abstract":"<p><p>Environmental stressors in aquatic organisms can be assessed using a bioenergetic approach based on the evaluation of changes in their physiological parameters. We evaluated the chronic effects of cadmium (Cd<sup>2+</sup>) on the energy balance as well as the survival, growth, metabolism, nitrogen excretion, hepatosomatic index, oxidized energy substrate, and osmoregulation of the shrimp Penaeus vannamei with the hypothesis that the high energy demand related to the homeostatic regulation of Cd<sup>2+</sup>could disrupt the energy balance and as a consequence, their physiological functions. The shrimp exposed to Cd<sup>2+</sup> had higher mortality (30%), directed more energy into growth (33% of energy intake), ingested 10% more energy, and defecated less than control animals. Cd<sup>2+</sup> exposure caused a tendency to decrease metabolism and ammonia excretion but did not alter the hepatosomatic index, type of energy substrate oxidized, and the hyperosmorregulatory pattern of the species. The Cd<sup>+2</sup> exposure may have induced a trade-off response because there was a growth rate increase accompanied by increased mortality.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-06-27DOI: 10.1007/s00244-024-01077-7
Limin Dang
A set of quantum chemical descriptors (molecular polarization, heat capacity, entropy, Mulliken net charge of the most positive hydrogen atom, APT charge of the most negative atom and APT charge of the most positive atom with hydrogen summed into heavy atoms) was successfully used to establish the classification models for the toxicity pLC50 of pesticides in Americamysis bahia. The optimal random forest model (Class Model A) yielded predictive accuracy of 100% (training set of 217 pesticides), 95.8% (test set of 72 pesticides) and 99.0% (total set of 289 pesticides), which were very satisfactory, compared with previous classification models reported for the toxicity of compounds in aquatic organisms. Therefore, it is reasonable to apply the quantum chemical descriptors associated with molecular structural information on molecular bulk, chemical reactivity and weak interactions, to develop classification models for the toxicity pLC50 of pesticides in A. bahia.
{"title":"Classification Model of Pesticide Toxicity in Americamysis bahia Based on Quantum Chemical Descriptors.","authors":"Limin Dang","doi":"10.1007/s00244-024-01077-7","DOIUrl":"10.1007/s00244-024-01077-7","url":null,"abstract":"<p><p>A set of quantum chemical descriptors (molecular polarization, heat capacity, entropy, Mulliken net charge of the most positive hydrogen atom, APT charge of the most negative atom and APT charge of the most positive atom with hydrogen summed into heavy atoms) was successfully used to establish the classification models for the toxicity pLC<sub>50</sub> of pesticides in Americamysis bahia. The optimal random forest model (Class Model A) yielded predictive accuracy of 100% (training set of 217 pesticides), 95.8% (test set of 72 pesticides) and 99.0% (total set of 289 pesticides), which were very satisfactory, compared with previous classification models reported for the toxicity of compounds in aquatic organisms. Therefore, it is reasonable to apply the quantum chemical descriptors associated with molecular structural information on molecular bulk, chemical reactivity and weak interactions, to develop classification models for the toxicity pLC<sub>50</sub> of pesticides in A. bahia.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01Epub Date: 2024-05-14DOI: 10.1007/s00244-024-01064-y
Kevin Flynn, Michelle Le, Monique Hazemi, Adam Biales, David C Bencic, Brett R Blackwell, Kendra Bush, Robert Flick, John X Hoang, John Martinson, Mackenzie Morshead, Kelvin Santana Rodriguez, Emma Stacy, Daniel L Villeneuve
It is postulated that below a transcriptomic-based point of departure, adverse effects are unlikely to occur, thereby providing a chemical concentration to use in screening level hazard assessment. The present study extends previous work describing a high-throughput fathead minnow assay that can provide full transcriptomic data after exposure to a test chemical. One-day post-hatch fathead minnows were exposed to ten concentrations of three representatives of four chemical modes of action: organophosphates, ecdysone receptor agonists, plant photosystem II inhibitors, and estrogen receptor agonists for 24 h. Concentration response modeling was performed on whole body gene expression data from each exposure, using measured chemical concentrations when available. Transcriptomic points of departure in larval fathead minnow were lower than apical effect concentrations across fish species but not always lower than toxic effect concentrations in other aquatic taxa like crustaceans and insects. The point of departure was highly dependent on measured chemical concentration which were often lower than the nominal concentration. Differentially expressed genes between chemicals within modes of action were compared and often showed statistically significant overlap. In addition, reproducibility between identical exposures using a positive control chemical (CuSO4) and variability associated with the transcriptomic point of departure using in silico sampling were considered. Results extend a transcriptomic-compatible fathead minnow high-throughput assay for possible use in ecological hazard screening.
据推测,低于以转录组为基础的起始点,就不太可能出现不良影响,从而提供了用于筛选级危害评估的化学浓度。本研究扩展了之前的工作,描述了一种高通量黑头鲦鱼检测方法,该方法可在暴露于测试化学品后提供完整的转录组数据。将孵化后一天的黑头鲦鱼暴露于四种化学作用模式的三种代表物质(有机磷、蜕皮激素受体激动剂、植物光合系统 II 抑制剂和雌激素受体激动剂)的十种浓度下 24 小时。在所有鱼类物种中,黑头鲦幼体的转录组出发点均低于顶端效应浓度,但并不总是低于甲壳类和昆虫等其他水生类群的毒性效应浓度。出发点在很大程度上取决于测量到的化学物质浓度,这些浓度通常低于标称浓度。对不同作用模式的化学品之间的差异表达基因进行了比较,结果往往显示出统计学上的显著重叠。此外,还考虑了使用阳性对照化学物(CuSO4)在相同暴露条件下的重现性,以及使用硅学取样与转录组出发点相关的变异性。研究结果扩展了与转录组兼容的黑头鲦鱼高通量检测方法,可用于生态危害筛选。
{"title":"Comparing Transcriptomic Points of Departure to Apical Effect Concentrations For Larval Fathead Minnow Exposed to Chemicals with Four Different Modes Of Action.","authors":"Kevin Flynn, Michelle Le, Monique Hazemi, Adam Biales, David C Bencic, Brett R Blackwell, Kendra Bush, Robert Flick, John X Hoang, John Martinson, Mackenzie Morshead, Kelvin Santana Rodriguez, Emma Stacy, Daniel L Villeneuve","doi":"10.1007/s00244-024-01064-y","DOIUrl":"10.1007/s00244-024-01064-y","url":null,"abstract":"<p><p>It is postulated that below a transcriptomic-based point of departure, adverse effects are unlikely to occur, thereby providing a chemical concentration to use in screening level hazard assessment. The present study extends previous work describing a high-throughput fathead minnow assay that can provide full transcriptomic data after exposure to a test chemical. One-day post-hatch fathead minnows were exposed to ten concentrations of three representatives of four chemical modes of action: organophosphates, ecdysone receptor agonists, plant photosystem II inhibitors, and estrogen receptor agonists for 24 h. Concentration response modeling was performed on whole body gene expression data from each exposure, using measured chemical concentrations when available. Transcriptomic points of departure in larval fathead minnow were lower than apical effect concentrations across fish species but not always lower than toxic effect concentrations in other aquatic taxa like crustaceans and insects. The point of departure was highly dependent on measured chemical concentration which were often lower than the nominal concentration. Differentially expressed genes between chemicals within modes of action were compared and often showed statistically significant overlap. In addition, reproducibility between identical exposures using a positive control chemical (CuSO<sub>4</sub>) and variability associated with the transcriptomic point of departure using in silico sampling were considered. Results extend a transcriptomic-compatible fathead minnow high-throughput assay for possible use in ecological hazard screening.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140920957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01Epub Date: 2024-05-22DOI: 10.1007/s00244-024-01066-w
Benoit Lalonde, Christine Garron
Alkylphenol ethoxylates comprise of many anthropogenic chemicals such as nonylphenol (NP), octylphenol (OP) and nonylphenol ethoxylates (NPEOs). The objectives of this study were to assess the frequency and magnitude of detections of 4-NP, OP and NPEOs in Canadian sediment downstream of textile associated municipal wastewater treatment plants (MWWTPs) to determine if regulatory actions have had a beneficial impact on the receiving environment. Surficial sediments were obtained in four locations in the province of Québec (Canada) and were analyzed for nonylphenol, nonylphenol monoethoxylates (NP1EO), nonylphenol diethoxylates (NP2EO) and octylphenol from 2015 to 2018. Individual concentrations of the compounds varied from non detect to 419 ng/g. Of the four compounds analyzed, NP was detected the most frequently with a 75% detection rate while OPs were not detected in any of the samples. Since the Canadian regulatory actions have drastically reduced NP/NPEOs usage in textile mill factories and manufactured products, the potential source of these compounds in sediment for this study could stem from the outfall from the MWWTPs but not related to textile mills as well as from the usage of these compounds as formulants in pesticide products. Lastly, there were no exceedances to the Canadian Sediment Quality guideline toxic equivalency approach (TEQ) of 1400 ng/g or the 1310 ng/g guideline for NP in freshwater sediment from the European Scientific Committee on Health, Environmental and Emerging Risks. We hypothesize that the significant concentrations of these compounds in sediment may be a relevant and continuous source of 4NP in surface waters due to resuspension of sediment in the water column.
{"title":"NP, OP and Derivatives in Freshwater Sediment Downstream of Textile Associated Municipal Wastewater Discharges.","authors":"Benoit Lalonde, Christine Garron","doi":"10.1007/s00244-024-01066-w","DOIUrl":"10.1007/s00244-024-01066-w","url":null,"abstract":"<p><p>Alkylphenol ethoxylates comprise of many anthropogenic chemicals such as nonylphenol (NP), octylphenol (OP) and nonylphenol ethoxylates (NPEOs). The objectives of this study were to assess the frequency and magnitude of detections of 4-NP, OP and NPEOs in Canadian sediment downstream of textile associated municipal wastewater treatment plants (MWWTPs) to determine if regulatory actions have had a beneficial impact on the receiving environment. Surficial sediments were obtained in four locations in the province of Québec (Canada) and were analyzed for nonylphenol, nonylphenol monoethoxylates (NP<sub>1</sub>EO), nonylphenol diethoxylates (NP<sub>2</sub>EO) and octylphenol from 2015 to 2018. Individual concentrations of the compounds varied from non detect to 419 ng/g. Of the four compounds analyzed, NP was detected the most frequently with a 75% detection rate while OPs were not detected in any of the samples. Since the Canadian regulatory actions have drastically reduced NP/NPEOs usage in textile mill factories and manufactured products, the potential source of these compounds in sediment for this study could stem from the outfall from the MWWTPs but not related to textile mills as well as from the usage of these compounds as formulants in pesticide products. Lastly, there were no exceedances to the Canadian Sediment Quality guideline toxic equivalency approach (TEQ) of 1400 ng/g or the 1310 ng/g guideline for NP in freshwater sediment from the European Scientific Committee on Health, Environmental and Emerging Risks. We hypothesize that the significant concentrations of these compounds in sediment may be a relevant and continuous source of 4NP in surface waters due to resuspension of sediment in the water column.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01Epub Date: 2024-05-18DOI: 10.1007/s00244-024-01067-9
William F Mills, Paco Bustamante, Francisco Ramírez, Manuela G Forero, Richard A Phillips
Mercury (Hg) is an environmental contaminant that can negatively impact the health of humans and wildlife. Albatrosses and large petrels show some of the highest levels of Hg contamination among birds, with potential repercussions for reproduction and survival. Here, body feather total Hg (THg) concentrations were determined in breeding adults of five species of albatrosses and large petrels in the foraging guild at South Georgia during the mid-2010s. We tested the effects of species, sex and trophic ecology (inferred from stable isotopes) on THg concentrations and compared our results with published values from past decades. Feather THg concentrations differed significantly among species (range: 1.9-49.6 µg g-1 dw), and were highest in wandering albatrosses Diomedea exulans, intermediate in black-browed albatrosses Thalassarche melanophris and northern giant petrels Macronectes halli, and lowest in southern giant petrels M. giganteus and white-chinned petrels Procellaria aequinoctialis. Females were more contaminated than males in all species, potentially due to differences in distributions and diet composition. Across species, THg concentrations were not correlated with feather δ13C or δ15N values, implying that species effects (e.g., breeding and moulting frequencies) may be more important than trophic effects in explaining feather THg concentrations in this foraging guild. Within species, the only significant correlation was between THg and δ13C in wandering albatrosses, which could reflect higher Hg exposure in subtropical waters. Comparisons with THg concentrations from past studies, which reflect contamination from 10 to > 60 years ago, revealed considerable annual variation and some evidence for increases over time for wandering and black-browed albatrosses since before 1950 and from the late 1980s, respectively.
{"title":"Mercury Concentrations in Feathers of Albatrosses and Large Petrels at South Georgia: Contemporary Patterns and Comparisons with Past Decades.","authors":"William F Mills, Paco Bustamante, Francisco Ramírez, Manuela G Forero, Richard A Phillips","doi":"10.1007/s00244-024-01067-9","DOIUrl":"10.1007/s00244-024-01067-9","url":null,"abstract":"<p><p>Mercury (Hg) is an environmental contaminant that can negatively impact the health of humans and wildlife. Albatrosses and large petrels show some of the highest levels of Hg contamination among birds, with potential repercussions for reproduction and survival. Here, body feather total Hg (THg) concentrations were determined in breeding adults of five species of albatrosses and large petrels in the foraging guild at South Georgia during the mid-2010s. We tested the effects of species, sex and trophic ecology (inferred from stable isotopes) on THg concentrations and compared our results with published values from past decades. Feather THg concentrations differed significantly among species (range: 1.9-49.6 µg g<sup>-1</sup> dw), and were highest in wandering albatrosses Diomedea exulans, intermediate in black-browed albatrosses Thalassarche melanophris and northern giant petrels Macronectes halli, and lowest in southern giant petrels M. giganteus and white-chinned petrels Procellaria aequinoctialis. Females were more contaminated than males in all species, potentially due to differences in distributions and diet composition. Across species, THg concentrations were not correlated with feather δ<sup>13</sup>C or δ<sup>15</sup>N values, implying that species effects (e.g., breeding and moulting frequencies) may be more important than trophic effects in explaining feather THg concentrations in this foraging guild. Within species, the only significant correlation was between THg and δ<sup>13</sup>C in wandering albatrosses, which could reflect higher Hg exposure in subtropical waters. Comparisons with THg concentrations from past studies, which reflect contamination from 10 to > 60 years ago, revealed considerable annual variation and some evidence for increases over time for wandering and black-browed albatrosses since before 1950 and from the late 1980s, respectively.</p>","PeriodicalId":8377,"journal":{"name":"Archives of Environmental Contamination and Toxicology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140954790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}