Asian Journal of Biochemistry, Genetics and Molecular Biology最新文献

{"title":"Microarray cDNA Dataset Analysis Reveals Potential Genes Associated with Type 2 Diabetes Mellitus for Further Treatment Exploration","authors":"S. Muhammad, W. Raza, Saba Ghafoor, Sania Sadeeq, Syeda Zahra Abbas, S. Noor","doi":"10.9734/ajbgmb/2023/v14i4321","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i4321","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is an intricate and inadequately treatable metabolic disorder that requires modified treatment by identifying genetic variants as potential drug targets. In this study, we performed the system-level genetic analysis of the T2DM-related cDNA dataset and revealed 5 significant differentials expressed genes (DEGs) including ABRA, CYR61, NR4A1, KY, and TMEM131 as source genes. Among, these genes, 3 were down-regulated and 2 up-regulated. The biological function and gene ontology showed the association of these genes with cell apoptosis, cell communication, signal transduction, and insulin resistance. These genes are majorly expressed in multiple tissues specifically the brain, lungs, pancreas, and immune cells. The protein-protein network revealed the interaction of these source genes with important signature proteins including FOS, IGFN1, UBC, CTNB1, ITB5, JUN, HIF1A, p53, and other important genes. This study would be helpful to understand the etiology of T2DM and also improve the development of new drug treatments by identification of genes associated with T2DM.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82434719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Oxidative Stress Status in those with Diabetes and Hypertension Attending Jos University Teaching Hospital (JUTH), Nigeria","authors":"Obot Uduakobong Blessing, Adugbe Abigail, Solomon D. Mariam, G. Y. Samuel","doi":"10.9734/ajbgmb/2023/v14i4320","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i4320","url":null,"abstract":"Diabetes is a serious, long-term condition with a major impact on the lives and wellbeing of individuals, families, and societies worldwide. This study aimed to investigate the impact of oxidative stress on individuals with type 2 diabetes mellitus (T2DM) and hypertension who were receiving care at the Endocrinology unit of Jos University Teaching Hospital (JUTH). A total of 54 subjects, comprising both males and females, were randomly selected for the study. The subjects included 27 patients diagnosed with T2DM and hypertension, as well as 27 healthy volunteers who were recruited as a control group. The study focused on assessing various parameters related to oxidative stress and antioxidant enzyme activity. The evaluation of oxidant status involved measuring Malondialdehyde (MDA) levels, which serve as a marker for lipid peroxidation, as well as Glycated hemoglobin (HbA1c) levels and Lipid profile. Additionally, the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH), was examined. Demographic information, lifestyle factors, and clinical assessments were also collected during the study. The results showed that HbA1c levels were significantly higher in diabetic patients compared to the healthy participants. Furthermore, the diabetic group exhibited higher MDA activity, indicating increased lipid peroxidation, compared to the control group (P = 0.001). In terms of antioxidant enzyme activity, the diabetic group showed elevated levels of SOD, CAT, and GSH compared to the control group (P = 0.001). Regarding lifestyle factors, there was a significant difference (P = 0.001) in lipid profiles between diabetic and non-diabetic individuals who engaged in regular exercise compared to those who did not exercise. Similarly, diabetic individuals who had consultations with a registered dietician or an Endocrinologist also demonstrated significantly improved lipid profiles compared to those who did not seek professional advice. Notably, diabetics who exercised regularly and received dietary guidance exhibited significantly higher levels of HDL compared to those who did not engage in exercise or receive dietary counseling. In conclusion, this study highlighted the association between oxidative stress and T2DM with hypertension. The findings emphasized the importance of managing oxidative stress through lifestyle modifications, such as regular exercise and dietary interventions, in improving lipid profiles and potentially mitigating the impact of diabetes on individuals' health and well-being.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80416055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Environmentally Safe Production of Xylanases by Fusarium sp. EA 1.3.1 Using Agroindustrial Residues: Biochemical Characterization and Potential Applications","authors":"G. O. Marinho, Eloísa A. Nogueira, T. Pasin, T. Oliveira, J. Roa, D. Nelson, Vivian Machado Benassi","doi":"10.9734/ajbgmb/2023/v14i4319","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i4319","url":null,"abstract":"Renewable energy-related biotechnologies such as biofuels produced from low-cost residual sources that represent clean technologies have become a partial solution to environmental problems. We sought to optimize the cultivation parameters of the fungus Fusarium sp. EA1.3.1 and biochemically characterize the naturally produced xylanases from the fungus. The development of the fungus was analyzed considering the variations in the resources available and by biochemical analysis of the crude extract. The composition and duration of the cultivation, nitrogen source, carbon source, salt solution, and initial pH of the medium were evaluated. The maximum xylanolytic production was obtained in Khanna medium enriched with a CP salts solution during four days of culture using yeast extract, wheat bran, and an initial pH of 8.5 for the culture medium. The optimum temperature and pH were 65°C and 6.5, respectively, for the xylanase activity from Fusarium sp. EA 1.3.1. The enzymatic extract presented general stability at 50°C, keeping 75% of its activity after 90 minutes of incubation, and its activity decreased to 20-40% with exposure to higher temperatures (60-70°C). The enzyme also presented high stability at pH 5.0 after 90 minutes, maintaining 85% of its relative activity. Thus, the isolated fungus presents high potential for xylanase production with desired biochemical and biophysical properties for industrial application.\u0000\u0000 ","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78554522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Body Mass Index and Abdominal Obesity in Bayelsa State with Associated Interleukin-2 Gene Expression","authors":"F. Ogbole, Evans Egereghe Sanugba","doi":"10.9734/ajbgmb/2023/v14i4318","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i4318","url":null,"abstract":"Aims: The aim of the present study was to evaluate the distribution, prevalence and determinants of obesity, overweight and abdominal obesity in Bayelsa State, Nigeria as well as associated IL-2 gene expression. \u0000Study Design: A community based cross sectional study design was employed to randomly select 420 participants (aged 18 – 65 years) for the study. \u0000Methodology: The study participants were grouped into normal, overweight and general obesity based on their body mass index (BMI) and sub-grouped into normal and abdominal obesity based on their waist circumference. Weighing scale, metre rule and measuring tape were used to measure weight, height and waist circumference respectively. Real time PCR was used to quantify IL-2 gene expression. \u0000Results: The BMI distribution of adults in Bayelsa State was symmetrical around the mean (27.25 ± 4.12). Mean waist circumference was 95.27 ± 15.92 (men 97.51 ± 12.38; women 93.41 ± 18.15 cm).  The prevalence of obesity, overweight and abdominal obesity were 26.2% (male, 10.7%; females, 15.5%), 35.2% (males, 16.4%; female, 18.8%), and 62.9% (males, 28.1% females, 34.8%) respectively. Compared with the normal group, a significant upregulation of IL-2 gene expression was found in the overweight, general obesity and abdominal obesity groups (p < 0.05). Significant association of the following determinants with obesity was found: both sexes, age group 36 - 55 years, working as small and medium scale business entrepreneurs and civil servants. Age group 56 – 65 years and female sex were associated with abdominal obesity. \u0000Conclusion: The present study found a high prevalence of overweight, general obesity and abdominal obesity in Bayelsa State and associated over expression of IL-2 gene. Findings may have implication for IL-2 based biomarker and drug development. Interventions to reduce BMI and waist circumference in Bayelsa State is emphasized in the present study.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84831650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomolecular Features of COVID-19 in Hodeidah, Yemen","authors":"Mohammed Amood Al Kamarany, Tarik Abdulkarim, Mahfouz Nasser","doi":"10.9734/ajbgmb/2023/v14i3317","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i3317","url":null,"abstract":"Background: The biomolecular technique namely Real-Time Polymerase Chain Reaction (RT-PCR) is very important in confirmation of coronavirus disease 2019 (COVID-19) but in progressive infection and predication of the illness severity is not used. \u0000Objective: Therefore, the study aimed to determine the relationship between the viral load of COVID-19 infection and the severity of illness based on cycle threshold (Ct). \u0000Methodology: The research was designed in a case series study. The study included 60 patients that were confirmed by the RT-PCR test with COVID-19 and divided into two major groups. The first major group was mild and moderate cases (n:20)  that were treated at home (outpatient) and the second major group was severe and critical cases (n:40) that were treated in the isolation center (inpatient). \u0000Results: The results showed no relationship between the viral load and severity of illness, where the minimum Ct was 14, and the maximum Ct was 36. The mean of  Ct was  22 ± 7. On the other hand, the low Ct (high viral load) was reported in early detection cases.  In addition , 60%  (36 cases) of patients had low Ct (high viral  load ;  Ct ≤ 15  and ≤ 25  ) 40% of patients (24 cases)  had high Ct (low viral load ; Ct ≤ 26  and ≤ 39 ) that was more  than 25 Ct and less than 39 cycles . On the other mean , 95 % of first group cases (mild and moderate cases )  had high viral load  based on Ct -values  and 42.5 % of second group (severe and critical) had low viral load based on Ct -values. On the other hand, kinetic of viral load–based Ct where the viral load was reported in the first week as very high (low Ct 21) with a longitudinal assessment of RT-PCR test results in individuals requiring third –fourth weeks to clear COVID-19  RNA showed a significant reduction of the viral load in samples  (Ct values > 30).      \u0000Conclusion: The study concluded that several factors can affect the Ct of RT-PCR (onset date, collection technique, type of swab, sampling method). Briefly, the COVID-19 RT-PCR test cannot be used as a predictor of the severity of illness.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79491097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Genetic Diversity of Tamarind (Tamarindus indica) Accessions in Eastern Region of Kenya Using Inter Simple Sequence Repeat (ISSR) Markers","authors":"Mercy Liharaka Kidaha, Cornelius Wainana Mbathi, Fredah K. Rimberia Wanzala","doi":"10.9734/ajbgmb/2023/v14i3316","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i3316","url":null,"abstract":"There is scanty information on tamarind’s genetic diversity in Easter Kenya. The objective of this study was to determine the genetic diversity of 64 tamarind accessions from Eastern Kenya using 12 Inter Simple Sequence Repeat (ISSR) markers. DNA was extracted from the young apical leaves using modified CTAB method and amplified using standard PCR.  The data collected were scored as presence (1) or absence (0) of bands then compared to the 100bp ladder and analyzed using GeneAlex and R softwares. Only seven primers produced reproducible bands. A total of 46 alleles were produced for the 7 loci with an average of 6.6 per loci. Polymorphic information content (PIC) varied from 0.72 to 0.89 and genetic diversity varied from 0.74 to 0.9. The ISSR markers revealed effective polymorphism of 40.87 to 99.46% and the band  sizes varied from 100 to 1000 bp. Analysis of Molecular Variance (AMOVA) depicted high variation within the tamarind populations at 90% and the least variation of 10% among the population. The first 3 components of Principal coordinate analysis (PCoA) contributed 40.83% of the total variation. Hierarchical cluster analysis grouped the tamarind accessions into seven major distinct groups. Tamarind accessions were different within counties with minimal variations among counties, proving that genetic diversity exists among the tamarind accessions in the Eastern region of Kenya. High genetic diversity was evident among Embu accessions and least among Masinga accessions. Diversity in tamarind can be utilized in marker-assisted breeding and primer ISSR17899A and ISSRHB11 can be explored in studying genes that code for various traits in tamarind accessions.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76204036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Most Common Mutations in the CFTR Gene in the Population of Bosnia and Herzegovina","authors":"Mirela Mačkić-Ðurović, E. Kiseljaković, I. Aganović-Mušinović, Admir Rasumović","doi":"10.9734/ajbgmb/2023/v14i3315","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i3315","url":null,"abstract":"Aims: Cystic fibrosis is an autosomal recessive multisystem disease caused by a mutation of the CFTR gene. To date, more than 1900 mutations of this gene are known. Studies have shown that the most common mutation is delF508. In Bosnia and Herzegovina, the prevalence of individual mutations in the general population has not been thoroughly studied, so this study aimed to determine the prevalence of the mutation concerning the countries of the region and the rest of the world. \u0000Study Design: Retrospective study. \u0000Place and Duration of Study: Thirty-nine subjects with suspected Cystic fibrosis were referred to the Center for Genetics of the Medical Faculty in Sarajevo between 2018-2020. \u0000Methodology: 29 common CFTR gene mutations were analysed with the ELUCIGENE CF29 v2 kit (Elucigene Diagnostics, UK) using four multiplex PCR. \u0000Results: The most common mutation in our study was the F508 deletion, present in 14 subjects (73.68%). R347P and G542X mutations were confirmed in two subjects in the heterozygous state in combination with delF508 (M) 5.26% of each of these mutations. 621+1G>T was found in a homozygous state in one subject, while in another, it was in a heterozygous state in combination with delF508(M) mutation, 10.52%. Mutation 2184 delA was found in one subject in the homozygous state with a total frequency of 5.26%. \u0000Conclusion: Subjects with cystic fibrosis in Bosnia and Herzegovina are most often carriers of the delF508 mutation. Considering the existence of many mutations and that it is difficult to test them all, targeting the most common mutations in a clinical environment might help in approving therapy, and increasing patients’ quality of life.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"466 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83591915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Creatinine Determination: Comparison between Colorimetric (Jaffé) and Enzymatic Methods in Renal Transplant Recipients","authors":"Houda Kouame, Mohammed Benamo-Seghir, Ayoub Samih, Asmaa Morjan, Nabiha Kamal","doi":"10.9734/ajbgmb/2023/v14i3314","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i3314","url":null,"abstract":"Background: Serum creatinine is the most requested parameter in current practice in the context of functional exploration of the kidney. Its contribution to medical care is particularly decisive for kidney transplant patients. The aim of the study is to compare the colorimetric and enzymatic techniques available in our laboratory for the determination of creatinine in samples from kidney transplant patients. \u0000Methods: Prospective study carried out at the Biochemistry laboratory of the University Hospital Center (CHU) Ibn Rochd in Casablanca Morocco, over a period of 8 months, carried out on a series of 45 samples from kidney transplant patients who simultaneously benefited from a creatinine assay by colorimetric and enzymatic techniques. The samples were taken on dry tubes and analyzed on the Alinity ® Abbott analyzer. The statistical study of correlation was made by the SPSS software with calculation of the p-value and the coefficient of Pearson (r). \u0000Results: During the study period 45 samples were collected. Seventy percent of the prescriptions came from the Nephrology Department. The mean serum creatinine value by enzymatic technique was 39.04 ± 11.7 mg/l, and by colorimetric technique 38.24 ± 12.4 mg/l. The creatinine level by the two techniques was pathological in 69% of cases and normal in 31%. The correlation between the serum creatinine assay by enzymatic Versus colorimetric technique is positive with r=0.994 and P-value<0.001. \u0000Conclusion: According to literature, colorimetric techniques are sensitive to interference with pseudochromogenic substances, while enzymatic techniques are standardized due to their specificity and performance but remain more expensive. In our study the correlation between the two techniques was positive with a correlation equation: (y = 0.82 + 1*x). According to our results, no significant difference between the two techniques was observed in our laboratory.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73682277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Non Enzymatic Status in Antioxidant Capital in Senegalese Type 2 Diabetics","authors":"S. Thiam, M. Touré, A. Samba, A. A. Ardja, A. Ndiaye, N. Coly, I. Soumah, F. Diedhiou, H. Agossou, A. Tounkara, F. DIa, R. Ba, I. Faye, M. Gueye, N. Diouf, F. Cissé, A. Diatta, F. Diallo","doi":"10.9734/ajbgmb/2023/v14i3313","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i3313","url":null,"abstract":"Oxidative stress appears very early in the history of type 2 diabetes complications. It is associated to glucose oxidation but also to lipid peroxidation. This stress can be accentuated by a decrease in the antioxidant capacities in diabetics, especially if there are other comorbidities. The objective of this study was to evaluate the antioxidant capacity of Senegalese type 2 diabetics and to identify the associated risk factors. \u0000We conducted a prospective study in 40 persons with type 2 diabetes. Factors related to age, sex, BMI, and BP were determined. For biological parameters, we measured blood glucose at empty stomach, glycated haemoglobin, total cholesterol, HDL, triglycerides and LDL, urea and creatinine, uric acid, albumin, total bilirubin, copper, and zinc. \u0000The mean age of the population was 58±11.24 years with a predominance of subjects aged over 60 (52.5%). The sex ratio was 1.11 with 52.5% male. Antioxidant capital was reduced in 60% of patients, with a predominance of women (35%). This reduction was marked by hypoalbuminemia (32.5%), hypozincemia (22.5) and an increase in the Cu/Zn ratio (30%). A strong correlation was found between the Cu/Zn ratio and total cholesterol (r=0.911; p<0.0001) as well as LDL (r=1; p=0.0001). Dyslipidaemia was found to be the most associated comorbidity with decreased oxidative capacity with an RR of 1.4 (CI=0.79-2.35) for total hypercholesterolemia and an RR of 1.6 (CI=0.75-3.71) for hypertriglyceridemia. \u0000The antioxidant capital is reduced in type 2 diabetics, especially in dyslipidaemia conditions. Hygienic dietary measures and supplementation with trace elements such as zinc should prevent the complications of oxidative stress in diabetics.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83866807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Glycemic Index, Hypolipidemic and Hypoglycemic Activities of “osu une” on Alloxan Induced Diabetic Rats","authors":"N. Umerah, A. Asouzu","doi":"10.9734/ajbgmb/2023/v14i2312","DOIUrl":"https://doi.org/10.9734/ajbgmb/2023/v14i2312","url":null,"abstract":"Background: The incidence of diabetes has been on the increase due to increase in sedentary lifestyle together with increase in life expectancy. “Osu une” is a native meal in Anambra State, Nigeria used in the management of diabetics. There is no scientific based study/data to ascertain the effect on blood glucose level. \u0000Objective: The study investigated the glycemic index, hypolipidemic and hypoglycemic activities of “osu une” on Alloxan induced rats. \u0000Methods: The “osu une” was prepared, dried and blended. Twenty adult male Albino rats were grouped into four of five rats each based on their body weight. Alloxan at a dose of 42mg/kg was induced intravenously through tails of group 2-4 rats. Blood samples were collected from the ocular vein and analyzed for blood glucose and lipid profile on day 7, 14 and 28 using standard method. Ten healthy subjects aged between 24 -40 participated in this study. They were fed with the standard food (50 g glucose) on day one and the test foods on day two, after an overnight fast. Blood samples were taken at 0, 30, 60, 120, and 180 min after the food had been eaten. \u0000Results: The result showed that rats that received ‘osu une’ extract showed an increase in body weight from 130.70mg/kg to 146.20mg/kg, while rats that received glucophage tablets also showed an increase in body weight from 126.01mg/kg to 158.81mg/kg after inducing diabetes. The rats fed ‘osu une’ extract had a decrease in fasting blood glucose level, total cholesterol (TC), triglycerides, Low Density Lipoprotein (LDLc) and increase in High Density Lipoprotein (HDLc). The test diet had a low glycemic index of 9.59. \u0000Conclusion: The study shows that “osu une” can play a key role in the management of Diabetes Mellitus.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87952099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}