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Expression of CD30 in testis and Epididymis of adult mice CD30在成年小鼠睾丸和附睾中的表达
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647751
Y. Choo, S. Nam
CD30 is a member of tumor necrosis factor receptor (TNFR) superfamily and has pleiotropic functions including cell activation, proliferation, differentiation, and death, depending on cell types and stage of differentiation. Although CD30 expression has been described mainly in hematopoietic tissues, several types of nonhematopoietic tumors including embryonic carcinoma and germ‐cell tumors express CD30. We examined CD30 distribution in the testis and epididymis from wild type and CD30‐deficient mice. In the testis, spermatogonia, spermatocytes and Sertoli cells expressed CD30, but not in spermatids. Spermatogonia and spermatocytes near the basement membrane strongly reacted to anti‐CD30. In the epididymis, CD30 expression was exclusively observed in luminal epithelia and some interstitial cells. Taken together, these results show a spatio‐temporal regulation of CD30 expression in mouse testis and epididymis and suggest a possible role of CD30 in spermatogonia and spermatocytes.
CD30是肿瘤坏死因子受体(TNFR)超家族的一员,根据细胞类型和分化阶段的不同,具有细胞活化、增殖、分化和死亡等多种功能。虽然CD30主要在造血组织中表达,但包括胚胎癌和生殖细胞肿瘤在内的几种非造血肿瘤也表达CD30。我们检测了野生型和CD30缺失小鼠睾丸和附睾中CD30的分布。在睾丸中,精原细胞、精母细胞和支持细胞表达CD30,但在精子中不表达。靠近基底膜的精原细胞和精母细胞对抗CD30反应强烈。在附睾中,CD30仅在管腔上皮和部分间质细胞中表达。综上所述,这些结果显示了CD30在小鼠睾丸和附睾中的时空表达调控,并提示CD30可能在精原细胞和精母细胞中发挥作用。
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引用次数: 0
Administration of agonistic anti‐4–1 BB monoclonal antibody inhibits melanoma metastasis via IFN‐γ production 拮抗- 4-1 BB单克隆抗体通过IFN - γ的产生抑制黑色素瘤转移
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647743
S. Ju, Sang-C. Lee, M. Seok, Byung-Sam Kim
The purpose of this study was to analyze inhibitory effects of anti‐4–1 BBmonoclonal antibody on melanoma metastasis. The 4–1 BB (CD137) T cell molecule is amember of the TNF receptor family and its activation by either 4–1 BB ligand or antibody induces T cell activation and growth. In the present study, administration of anti‐4–1BB mAb induced inhibition of melanoma metastasis. Agonistic anti‐4–1 BB mAb induced not only CD8+4–1BB+ T cells but also CD8+IFN‐γ+ T cell population. In the presence of anti‐CD3 antibody, lymphocytes produced high levels of IFN‐γ and low levels of IL‐4 in anti‐4–1 BB mAb treated group. Exposure of melanoma cells to IFN‐γ induced expression of MHC‐I molecules. Thus, the increase in number of CD8+ T cells and enhanced MHC‐I expression on B16F10 cells by augmented IFN‐γ production in response to anti‐4–1 BB mAb may result in suppression of tumor growth and metastasis.
本研究的目的是分析抗- 4-1 bb克隆抗体对黑色素瘤转移的抑制作用。4-1 BB (CD137) T细胞分子是TNF受体家族的成员,其被4-1 BB配体或抗体激活可诱导T细胞活化和生长。在本研究中,抗- 4-1BB单抗可诱导黑色素瘤转移抑制。抗- 4-1 BB单抗不仅能诱导CD8+ 4-1BB + T细胞,还能诱导CD8+IFN - γ+ T细胞群。在抗CD3抗体存在的情况下,抗- 4 - 1 BB单抗处理组淋巴细胞产生高水平的IFN - γ和低水平的IL - 4。黑色素瘤细胞暴露于IFN‐γ诱导MHC‐I分子的表达。因此,在抗- 4-1 BB单抗的作用下,通过增加IFN - γ的产生,B16F10细胞上CD8+ T细胞数量的增加和MHC - I表达的增强可能导致肿瘤生长和转移的抑制。
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引用次数: 0
A new sponge of the genus Phorbas (Poecilosclerida: Hymedesmiidae) from Gageodo island (So‐Huksando), Korea 标题韩国加古多岛(So‐Huksando) Phorbas属海绵一新记录(水蛭目:水蛭科)
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647745
C. Sim, H. Kim
A taxonomic study on marine sponges was conducted with materials collected from Gageodo Island, Korea, from August 1999 to July 2001 by SCUBA diving. Among them, Phorbas gukhulensis n. sp. is new to science. Phorbas gukhulensis n. sp. is similar to P. fitictius Pulitzer‐Finali from which it differs in spicule size and growth form.
对1999年8月至2001年7月在韩国加裕岛采集的海洋海绵进行了分类研究。其中,Phorbas gukhulensis n. sp.是科学上的新物种。Phorbas gukhulensis n. sp.与P. fitictius Pulitzer‐Finali相似,但在针状体大小和生长形式上有所不同。
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引用次数: 2
Two new marine psammocinian sponges (Demospongiae: Dictyoceratida: Irciniidae) from Korea 文章题目韩国海生沙棘海绵二新种(Demospongiae: dictyoceratia: ircinciidae)
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647746
K. Lee, C. Sim
Two new species of the genus Psammocinia (Dictyoceratida, Irciniidae), P. conulosa n. sp. and P. ulleungensis n. sp., are described from Namhaedo Island and Ulleungdo Island, Korea. Psammocinia conulosa n. sp. seems to be close to both P. amodes Cook and Bergquist, 1998 and P. hawere Cook and Bergquist, 1996 on the basis of the skeletal structure. However, these three species are clearly separated by the following differences: Shape of P. amodes is spatulate and thin, with a broad blade narrowing to a semi‐cylindrical stalk; P. hawere forms cups with a shallow excavated bowl, and attached to the substratum by a narrow base. The whole surface of the new species is very finely conulose. Psammocinia ulleungensis n. sp. is similar to P. gageoensis Sim and Lee, 2001 in shape, but this species is easily distinguished from P. gageoensis by the simple skeletal structure.
报道了产自韩国南海道岛和郁陵岛的沙尘蝇属二新种(Dictyoceratida, ircinidae) P. conulosa n. sp和P. ulleungensis n. sp。在骨骼结构的基础上,Psammocinia conulosa n. sp.似乎与P. amodes Cook and Bergquist(1998)和P. hawere Cook and Bergquist(1996)接近。然而,这三种植物有以下明显的区别:P. amodes的形状是匙形的,薄的,宽的叶片缩小到一个半圆柱形的茎;P. hawere用一个浅挖的碗形成杯子,并通过一个狭窄的底部附着在基质上。这个新种的整个表面都有很细的圆锥花序。Psammocinia ulleungensis n. sp.在外形上与P. gageoensis Sim and Lee, 2001相似,但其骨骼结构简单,容易与P. gageoensis区分。
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引用次数: 1
Molecular DNA systematic analyses of East Asian mammals: Sequence Variation of cytochrome b Gene and Control Region of Mitochondrial DNA of Common Otter, Lutra lutra lutra L. (Mammalia, Carnivora) from Korea 东亚哺乳动物分子DNA系统分析:韩国普通水獭(哺乳动物,食肉目)细胞色素b基因序列变异及线粒体DNA控制区
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647755
H. Koh, Minyoung Yoo, Bae Geun Lee, Jeong Gyu Park
Sequences of cytochrome b gene and control region of mitochondrial DNA from Korean common otters (Lutra lutra lutra L.) were examined to provide the genetic information for the conservation of this subspecies. Two haplotypes and one haplotype were revealed in cytochrome b gene and control region, respectively. The available sequences of European common otter (L. l. lutra) from GenBank were compared together with those of Korean common otter in order to determine the degree of sequence variation between them. In cytochrome b gene sequences, two haplotypes from Korea and two haplotypes of Europe showed differences in 12 of 1,045 sites. The Tamura‐Nei nucleotide distances between two European haplotypes was 0.10% and those between two Korean haplotypes was also 0.10%, but those between Korean haplotypes and European ones ranged from 0.96% to 1.16%. In the control region, one Korean haplotype and seven European ones showed differences in seven of 300 sites; the Tamura‐Nei distances among seven European haplotypes were 0.34% to 1.01%, but those between Korean haplotype and European ones ranged from 1.01% to 1.69%. Although further molecular and morphological studies with specimens from eastern Asia including Amur region and northeast China are needed, it is possible that the Korean common otter might be closer or identical to the far‐eastern Asian common otter, L. I. amurensis Dybowski.
对韩国水獭(Lutra Lutra Lutra L.)的细胞色素b基因序列和线粒体DNA控制区进行了分析,以期为该亚种的保护提供遗传信息。细胞色素b基因和控制区分别显示2个单倍型和1个单倍型。将GenBank中欧洲普通水獭(l.l.l utra)的可用序列与韩国普通水獭的序列进行比较,以确定它们之间的序列差异程度。在细胞色素b基因序列中,韩国的2个单倍型和欧洲的2个单倍型在1045个位点中有12个存在差异。两种欧洲单倍型之间的Tamura - Nei核苷酸距离为0.10%,两种韩国单倍型之间的Tamura - Nei核苷酸距离为0.10%,而韩国单倍型与欧洲单倍型之间的Tamura - Nei核苷酸距离为0.96% ~ 1.16%。在对照区,300个位点中有7个位点出现了1个韩国单倍型和7个欧洲单倍型的差异;7个欧洲单倍型的Tamura - Nei距离为0.34% ~ 1.01%,而韩国单倍型与欧洲单倍型的Tamura - Nei距离为1.01% ~ 1.69%。虽然需要对包括黑龙江地区和中国东北在内的东亚地区的标本进行进一步的分子和形态学研究,但韩国普通水獭可能与远东普通水獭l.i. amurensis Dybowski更接近或相同。
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引用次数: 7
Two new species of siphonostomatoid copepods (crustacea) associated with the stoloniferan coral tubipora musica (Linnaeus) from Madagascar 与马达加斯加匍匐茎珊瑚(Linnaeus)有亲缘关系的两新种(甲壳纲)
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647750
Il-Hoi Kim
Two new species of siphonostomatoid copepods, Asterocheres tubiporae n. sp. and Entomopsyllus stocki n. sp., associated with the stoloniferan coral Tubipora musica (Linnaeus) are described from Madagascar. Asterocheres tubiporae is characterized by the possession of a large posteroventral process on the caudal ramus and the elongated free segment of leg 5. Entomopsyllus stocki is readily distinguished from its congeners by the possession in the female of 15‐segmented antennule and three spines plus two setae on the third exopodal segment of leg 4.
在马达加斯加发现了与匍匐茎珊瑚有亲缘关系的两新种:Asterocheres tubiporae n. sp和entomopsilus stocki n. sp。管状裂孔的特点是在尾支上有一个大的后腹突和5号腿的延长的自由节。stockki昆虫很容易与它的同族昆虫区分开来,因为雌性昆虫在第4条腿的第三节上具有15节的触角和3根刺和2根刚毛。
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引用次数: 12
Structural characterization of mouse HAUSP, a proteolysis regulator of p53 p53蛋白水解调节因子小鼠HAUSP的结构表征
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647752
Hye-Jin Lee, Kyong-Jai Yoo, K. Baek
The tumor suppressor protein p53 is stabilized by the herpes‐virus‐associated ubiquitin‐specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site‐directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.
肿瘤抑制蛋白p53被疱疹病毒相关泛素特异性蛋白酶(HAUSP)稳定,HAUSP是一种去泛素化酶。我们之前分离并鉴定了HAUSP的小鼠同源物mHAUSP。mHAUSP cDNA全长3312 bp,编码1103个氨基酸,分子量约为135 kDa,包含高度保守的Cys、Asp (I)、His和Asn/Asp (II)结构域。在本研究中,我们对Cys盒、QQD盒和His盒中的6个保守氨基酸(Cys224、Gln231、Asp296、His457、His465和Asp482)进行了定点诱变。有趣的是,保守的Gln 231对mHAUSP的催化活性并不是必需的。然而,mHAUSP的去泛素化活性需要其他保守的氨基酸。我们进行了异肽酶测定,证实了mHAUSP能够从泛素化底物中去除泛素。此外,我们观察到mHAUSP诱导HeLa细胞凋亡。
{"title":"Structural characterization of mouse HAUSP, a proteolysis regulator of p53","authors":"Hye-Jin Lee, Kyong-Jai Yoo, K. Baek","doi":"10.1080/12265071.2004.9647752","DOIUrl":"https://doi.org/10.1080/12265071.2004.9647752","url":null,"abstract":"The tumor suppressor protein p53 is stabilized by the herpes‐virus‐associated ubiquitin‐specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site‐directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"8 1","pages":"205 - 212"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2004.9647752","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new sponge of the genus haliclona (gellius) (haplosclerida: Chalinidae) from gageodo island (so‐huksando), Korea 文章标题韩国加古多岛(so‐huksando)海螺属海绵一新记述(单孔虫目:海螺科)
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647757
H. Kim, C. Sim
A taxonomic study on marine sponges was conducted by SCUBA diving at Gageodo Island, Korea from August 1999 to July 2001. Among them, Haliclona (Gellius) coreana n. sp. turned out to be new to science. This new species seems to be close to H. (G.) arcoferus Vosmaer, 1885, based on the type of spicules. However, it is different in size of toxa and growth form. Toxa of the new species is half in length compared to H. (G.) arcoferus. The growth form is jar shaped in this new species, but flat in H. (G.) arcoferus.
1999年8月至2001年7月,在韩国加裕岛用水肺潜水对海绵进行了分类研究。其中,海螺属(Gellius) coreana n. sp.对科学来说是新的。根据针状体的类型,这一新种似乎与1885年的H. (G.) arcoferus Vosmaer接近。但是,它们在毒虫的大小和生长形式上是不同的。新种弓形虫的长度为弓形虫的一半。该新种的生长形态为瓶状,但在H. (G.) arcoferus中呈扁平状。
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引用次数: 2
Comparative analysis of the responses to intruders with anxiety‐related behaviors of mouse 小鼠焦虑相关行为对入侵者反应的比较分析
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647764
Sanghyeon Kim, Eunchai Kang, Chankyu Park
Anxiety in mice can be measured by behavioral reactivity to social or non‐social Stressors. These behaviors were compared by performing the resident‐intruder test (social) as well as the light‐dark transition and open‐field tests (non‐social) for the FVB, C57BL/6, and BALB/c lines of mouse. The three inbred lines showed significant differences in their responses to intruder mice. Three factors, accounting for about 68% of the total variance, were extracted from the scores obtained from the three behavioral tests. The first two major factors are primarily associated with the anxiety‐related behaviors. One includes anxiety behaviors with a locomotive basis, while the other includes defecation measured in both anxiety tests. The third factor explains the three social behaviors, facial investigation, ano‐genital investigation, and following, observed in the resident intruder test, although facial investigation is also moderately associated with the second factor. The results indicate that the behavioral responses to an intruder share a component distinct from anxiety‐related behaviors.
小鼠的焦虑可以通过对社会或非社会压力源的行为反应来测量。这些行为通过对FVB、C57BL/6和BALB/c系小鼠进行居民-入侵者测试(社会性)以及明暗转换和开放场测试(非社会性)进行比较。这三种自交系对入侵小鼠的反应有显著差异。从三个行为测试的得分中提取了三个因素,约占总方差的68%。前两个主要因素主要与焦虑相关行为有关。其中一个包括基于机车的焦虑行为,而另一个包括在两个焦虑测试中测量的排便。第三个因素解释了常住入侵者测试中观察到的三种社会行为:面部调查、无生殖器调查和以下行为,尽管面部调查也与第二个因素有一定的关联。结果表明,对入侵者的行为反应与焦虑相关的行为有不同的组成部分。
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引用次数: 5
First larvae of Lebbeus comanthi and Thor amboinensis (Decapoda: Hippolytidae) hatched in the laboratory 在实验室里孵化出了第一批黄斑白蛉和黄斑白蛉(十足目:河马科)的幼虫
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647729
Hoi-Jeong Yang, J. Okuno
The decapodid stage of Lebbeus comanthi Hayashi and Okuno and the first zoea of Thor amboinensis (De Man) are described based on laboratory‐hatched eggs from females collected from Japan. The decapodid stage of L. comanthi is readily distinguished from that of L. groenlandicus by the carapace without anteroventral denticle and tooth behind rostrum, the absence of the antennal spine, the four‐segmented outer flagellum of the antennule, the absence of the palp of the mandible, and the telson with posterior margin concaved medially and without dorsolateral spine. The carapace with anteroventral denticle and the third abdominal somite distinctly curved in lateral view distinguish the first zoea of T. amboinensis from that of T. dobkini Chace and T. floridanus Kingsley. Larval characters of the genus Thor are summarized.
根据从日本收集的雌性实验室孵化的卵,描述了Lebbeus comanthi Hayashi和Okuno的十足期和Thor amboinensis (De Man)的第一个幼体。十足期的甲壳没有前腹突和齿,喙后没有触角棘,触角棘的外鞭毛有四个节段,下颌骨没有触须,端部后缘内侧凹,没有背外侧棘。腹前齿甲壳和腹部第三节体在侧面明显弯曲,将amboinensis与T. dobkini Chace和T. floridanus Kingsley区分开来。综述了托尔属昆虫的幼虫特征。
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引用次数: 7
期刊
Korean journal of biological sciences
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