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Communication of young black‐tailed gulls, larus crassirostris, in response to parent's behavior 幼黑尾鸥,larus crassirostris对父母行为的反应
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647763
Hoon Chung, Seokwan Cheong, Shi‐Ryong Park
In the breeding colony of black‐tailed gull, as nests of conspecific neighbors are very closely located, chicks are permanently exposed by sound and visual stimuli produced by adult conspecifics approaching their nests. The chicks, therefore, may need to learn ways to appropriately respond to their parents approach. In this study we experimentally manipulated sensory stimulation that is potentially provided by the parents to the offspring. Chicks incubated in the laboratory were exposed to a mew call of the conspecific adult. Then they were tested in three situations differing in sensory stimulation: 1) visual stimulation only, 2) auditory stimulation only, and 3) Simultaneous visual and auditory stimulations. We observed occurrence of different response of the chicks, which were categorized into three behaviors (begging call response, chirirah call and pecking behavior). We also investigated intensity of the chick's call in response to the different stimulations and the degree of response with age. The chicks exposed to only auditory stimulation made significantly more chirirah calls. The intensities (dB) of the mew call and chicks’ chirirah call were directly correlated. On the other hand, when chicks just saw the stuffed adult gull, they responded significantly more with a begging call and pecking behavior. In the situation of costimulation, the chicks responded with a begging call and pecking, but less frequently than visual stimulation only. The results suggest that young black‐tailed gulls use call repertories to properly respond to parents behavior. Such results suggest an evolutionary process for uncreasing their survival rate in a group breeding site.
在黑尾鸥的繁殖群体中,由于同种邻居的巢穴位置非常近,雏鸟会长期暴露在成年同种鸥接近其巢穴时产生的声音和视觉刺激下。因此,雏鸟可能需要学习如何恰当地回应父母的做法。在这项研究中,我们通过实验操纵了可能由父母提供给后代的感官刺激。在实验室里孵化的雏鸟被暴露在同种成年鸟的新叫声中。然后在三种不同感官刺激的情况下进行测试:1)仅视觉刺激,2)仅听觉刺激,3)视觉和听觉同时刺激。我们观察到雏鸟的不同反应,将其分为三种行为(乞求叫声反应、亲嘴叫声和啄食行为)。我们还研究了雏鸟对不同刺激的反应强度和随年龄的反应程度。只受到听觉刺激的雏鸟会发出更多的“基拉拉”叫声。新啼的强度(dB)与雏鸟的chirrirah叫声直接相关。另一方面,当雏鸟看到填充的成年海鸥时,它们的反应明显更多的是乞讨叫声和啄食行为。在共同刺激的情况下,雏鸟会发出乞求叫声和啄食,但频率低于单纯的视觉刺激。结果表明,幼黑尾鸥使用叫声库来对父母的行为做出适当的反应。这样的结果表明,在群体繁殖场所,有一个提高它们存活率的进化过程。
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引用次数: 2
Effect of different diets on growth and survival rates of snakehead (channa striata bloch, 1797) larvae 不同饵料对黑鱼幼鱼生长和存活率的影响
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647766
A. Abol-Munafi, B. Tarn, M. Ambak, P. Ismail
A study was conducted to determine growth and survival rates of snakehead (Channa striata) larvae. Live foods such as Artemia salina naupiii, Moina micrura and bloodworm and artificial diet were given to larvae from 1–15 days after hatching as the 1st phase and from 15–30 days as the 2nd phase. In the 1st phase, the growth and survival rates of larvae fed with Artemia nauplii were significantly different from larvae fed with Moina (p<0.05) with values of 28.5 mg, 49% and 26.7 mg, 31%, respectively. Meanwhile, all larvae fed with bloodworm (Chironomus sp.) or artificial diet (49% protein) died within 10 days of experiment. For the 2nd phase, growth of the fry fed with Artemia or Moina differed significantly from that fed with bloodworm or pellet (200.1, 187.7, 109.6 and 8.2 mg, respectively). Specific growth rate (SGR) of larvae fed with Moina was higher than that of larvae fed with Artemia (17.9 and 17.4% day‐1 respectively) in the 1st phase. In the 2nd phase, SGRs of larvae fed with Artemia (12.7% day‐1) or Moina (11.5% day‐1) were significantly higher than those of larvae fed with bloodworm (8.3% day‐1) or dry diet (6.1% day‐1). Generally, Artemia and Moina were suitable food for snakehead larvae during the first month of their life, and artificial diet was much less acceptable and resulted in poor growth and survival.
研究了黑鱼(Channa striata)幼虫的生长和存活率。幼虫孵化后1 ~ 15 d饲喂盐渍蒿(Artemia salina naupiii)、微纹蒿(Moina microura)、血虫等活饲料和人工饲料,作为第一阶段,15 ~ 30 d饲喂第二阶段。在第1阶段,与莫伊纳相比,采食无叶蒿幼虫的生长和存活率分别为28.5 mg、49%和26.7 mg、31%,差异显著(p<0.05)。同时,饲喂血虫(Chironomus sp.)或人工饲料(49%蛋白质)的幼虫均在10 d内死亡。在第二阶段,饲料中添加青蒿或莫伊娜的鱼苗生长与饲料中添加血虫或颗粒(分别为200.1、187.7、109.6和8.2 mg)的鱼苗生长差异显著。在第一期,Moina的特定生长率(SGR)高于Artemia,分别为17.9%和17.4% (d - 1)。在第二阶段,饲喂青蒿(12.7%)或莫伊纳(11.5%)的幼虫sgr显著高于饲喂血虫(8.3%)或干饲料(6.1%)的幼虫sgr。一般来说,青蒿和莫伊纳是蛇头幼虫在其生命的第一个月的适宜食物,人工饲料的可接受性差,导致其生长和成活率差。
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引用次数: 10
Effect of glucose, its analogs and some amino acids on pre‐steady state kinetics of ATP hydrolysis by PM‐ATPase of pathogenic yeast (candida albicans) 葡萄糖及其类似物和某些氨基酸对致病性酵母(白色念珠菌)PM - ATP酶水解ATP前稳态动力学的影响
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647765
B. Rashid, N. Manzoor, M. Amin, L. Khan
Fast kinetics of transient pH changes and difference spectrum formation have been investigated following mixing of ADP/ATP with partially purified plasma membrane PM‐ATPase of the pathogenic yeast Candida albicans in the presence of five nutrients: glucose, glutamic acid, proline, lysine, and arginine and two analogs of glucose: 2‐deoxy D‐glucose and xylose. Average hT‐absorption to release ratio, indicative of population of ATPase undergoing complete hydrolytic cycle, was found to be 0.27 for control. This ratio varied between 0.25 (proline) to 0.36 (arginine) for all other compounds tested, except for glucose. In the presence of glucose, H+‐absorption to release ratio was exceptionally high (0.92). While no UV difference spectrum was observed with ADP, mixing of ATP with ATPase led to a large conformationai change. Exposure to different nutrients restricted the magnitude of the conformationai change; the analogs of glucose were found to be ineffective. This suppression was maximal in the case of glucose (80%); with other nutrients, the magnitude of suppression ranged from 40–50%. Rate of H+‐absorption, which is indicative of E∼P complex dissociation, showed positive correlation with suppression of conformationai change only in the case of glucose and no other nutrient/analog. Mode of interaction of glucose with plasma membrane H+‐ATPase thus appears to be strikingly distinct compared to that of other nutrients/analogs tested. The results obtained lead us to propose a model for explaining glucose stimulation of plasma membrane H+‐ATPase activity.
在五种营养物质(葡萄糖、谷氨酸、脯氨酸、赖氨酸和精氨酸)和两种葡萄糖类似物(2‐脱氧D‐葡萄糖和木糖)的存在下,将ADP/ATP与部分纯化的质膜PM‐ATP酶混合,研究了瞬时pH变化和差异光谱形成的快速动力学。平均hT -吸收与释放比,表明atp酶群体完成了完整的水解循环,在对照中发现为0.27。除了葡萄糖外,所有其他化合物的这个比值在0.25(脯氨酸)到0.36(精氨酸)之间变化。在葡萄糖的存在下,H+‐的吸收与释放比异常高(0.92)。虽然没有观察到ADP的紫外差异光谱,但ATP与ATPase的混合导致了很大的构象变化。不同营养物质的暴露限制了构象变化的幅度;葡萄糖类似物被发现是无效的。这种抑制在葡萄糖的情况下最大(80%);与其他营养物质相比,抑制幅度在40-50%之间。表明E ~ P复合物解离的H+ -吸收率仅在葡萄糖而没有其他营养物质/类似物的情况下与抑制构象变化呈正相关。因此,葡萄糖与质膜H+ - atp酶的相互作用模式与其他营养物质/类似物的相互作用模式明显不同。所得结果使我们提出了一个解释葡萄糖刺激质膜H+‐atp酶活性的模型。
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引用次数: 15
The p110γ PI‐3 kinase is required for the mechanism by which the EphA8‐induced neurites are modulated by ephrin‐A5 engagement p110γ PI‐3激酶是EphA8诱导的神经突通过ephrin‐A5参与调节的机制所必需的
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647734
Soochul Park
This study provides evidence that expression of EphA8 receptor in NG108–15 cells results in a substantial increase in the number of neurite‐bearing cells. However, the EphA8‐induced neurite outgrowth does not require either ephrin‐A5 stimulation or ectopic expression of p110γPI‐3 kinase. In contrast, co‐expression of a lipid kinase‐inactive p110γ mutant together with EphA8 causes neurite retraction in the presence of ephrin‐A5 stimulation. This effect was not observed in the absence of ephrin‐A5 stimulation. Significantly, the tyrosine kinase activity of EphA8 is not important for either of these processes. Taken together, our results strongly suggest that p110γ PI‐3 kinase is critically involved in the regulatory process by which ephrin‐A5 exerts effects on the EphA8‐induced neurite outgrowth.
本研究提供了EphA8受体在NG108-15细胞中的表达导致神经突细胞数量的显著增加的证据。然而,EphA8诱导的神经突生长不需要ephrin - A5刺激或p110γPI - 3激酶的异位表达。相反,脂激酶失活的p110γ突变体与EphA8的共同表达会在ephrin - A5刺激下引起神经突缩回。在没有ephrin - A5刺激的情况下,没有观察到这种效应。值得注意的是,EphA8的酪氨酸激酶活性对这两个过程都不重要。综上所述,我们的研究结果强烈表明,p110γ PI‐3激酶在ephrin‐A5对EphA8诱导的神经突生长产生影响的调节过程中起着关键作用。
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引用次数: 0
First zoea of petrolisthes trilobatus (crustacea: Decapoda: Anomura: Porcellanidae) hatched in the laboratory 在实验室孵化的油石三叶虫(甲壳纲:十足纲:反常目:Porcellanidae)的第一个动物
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647758
H. Ko
The first zoea of Petrolisthes trilobatus Osawa, 1996, from a female collected in Japan is described and illustrated. Its morphological characteristics are compared with those of other known species of the genus Petrolisthes. The first zoea of P. trilobatus appears very similar to those of P. lamarckii, P. asiaticus and P. hastatus. However, it could be distinguished from the others in having a spine on the lower posterior margin of the carapace, three pairs of setae on the postero‐dorsal surface of the telson and minute spinules on the dorsal margins of the abdominal somites 2–5. Based on zoeal morphology, it is suggested that the zoeas of Petrolisthes could be divided into two groups: the first group (P. coccineus, P. moluccensis, P. lamarckii, P. trilobatus, P. asiaticus, P. hastatus, P. pubescens, P. tomentosus, and P. carinipes) and the second one (P. ohshimai, P. armatus, P. boscii, P. tridentatus, P. tonsorius, and P. japonicus).
本文描述了1996年在日本采集的三叶三叶虫(Petrolisthes triobatus Osawa)雌虫的第一个卵体。并将其形态特征与其他已知岩石属物种进行了比较。三叶弓形虫的第一虫群与拉马克弓形虫、亚洲弓形虫和hastatus弓形虫的第一虫群非常相似。然而,它与其他种类的区别在于,在甲壳的下后缘有一根棘,在跗关节的后背表面有三对刚毛,在腹部小体的背缘有微小的小刺2-5。根据动物形态划分,石鳖动物可分为两类:第一类(瓢虫P. coccineus、moluccensis、lamarckii、三叶虫P. trilobatus、亚洲虫P. hastatus、短毛虫P. pubescens、毛毛虫P. carinipes)和第二类(石岛虫P. armatus、波斯虫P. boscii、三叉虫P. tonsoorius、日本虫P. japonicus)。
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引用次数: 1
Cloning and expression of a cDNA AAPT3 encoding aminoalcoholphosphotransferase Isoform from Chinese Cabbage 大白菜氨基醇磷酸转移酶cDNA AAPT3基因的克隆与表达
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647741
K. Kim, Jong Ho Park, S. Cho
Aminoalcoholphosphotransferase catalyzes the synthesis of phosphatidylcholine and phosphatidylethanolamine from diacylglycerol plus a CDP‐aminoalcohol such as CDP‐choline or CDP‐ethanolamine. Previously we suggested the presence of possible isoforms of this enzyme from Chinese cabbage roots and now report the cDNA cloning and expression analysis of AAPT3 encoding a third isoform of aminoalcoholphosphotransferase (AAPT3). AAPT3 contains an open reading frame of 1,176 bp coding for a protein of 392 amino acids. It shares 96 and 95% identity with Chinese cabbage AAPT1 and AAPT2, respectively, at the deduced amino acid level. The results from reverse transcriptase‐polymerase chain reaction analysis indicate that expression of AAPT3 is up‐regulated by low temperature as well as AAPT1 and AAPT2.
氨基醇磷酸转移酶催化二酰基甘油加CDP -氨基醇(如CDP -胆碱或CDP -乙醇胺)合成磷脂酰胆碱和磷脂酰乙醇胺。在此之前,我们认为该酶可能存在于白菜根中,现在我们报道了编码第三种氨基醇磷酸转移酶(AAPT3)异构体的AAPT3的cDNA克隆和表达分析。AAPT3包含一个1176 bp的开放阅读框,编码392个氨基酸的蛋白。在推导出的氨基酸水平上,它与大白菜AAPT1和AAPT2的同源性分别为96%和95%。逆转录聚合酶链反应结果表明,低温上调了AAPT3的表达,也上调了AAPT1和AAPT2的表达。
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引用次数: 1
Expression of PACT and EIF2C2, Implicated in RNAi and MicroRNA pathways, in various human cell lines 与RNAi和MicroRNA通路相关的PACT和EIF2C2在多种人类细胞系中的表达
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647753
Yong Sun Lee, Yesu Jeon, Jonghoon Park, D. Hwang, Anindya Dutta
MicroRNA and siRNA (small interfering RNA), representative members of small RNA, exert their effects on target gene expression through association with protein complexes called miRNP (microRNA associated ribonucleoproteins) and RISC (RNA induced silencing complex), respectively. Although the protein complexes are yet to be fully characterized, human EIF2C2 protein has been identified as a component of both miRNP and RISC. In this report, we raised antiserum against EIF2C2 in order to begin understanding the protein complexes. An immunoblot result indicates that EIF2C2 protein is ubiquitously expressed in a variety of cell lines from human and mouse. EIF2C2 protein exists in both cellular compartments, as indicated by an immunoblot assay with a nuclear extract and a cytosolic fraction (S100 fraction) from HeLa S3 lysate. Depletion of EIF2C1 or EIF2C2 protein resulted in a decrease of microRNA, suggesting a possible role of these proteins in microRNA stability or biogenesis. We also prepared antiserum against dsRNA binding protein PACT, whose homologs in C. elegans and Drosophila are known to have a role in the RNAi (RNA interference) pathway. The expression of PACT protein was also observed in a wide range of cell lines.
作为小RNA的代表成员,MicroRNA和siRNA分别通过与miRNP (MicroRNA associated ribonucleoproteins, MicroRNA相关核糖核蛋白)和RISC (RNA induced silencing complex, RNA诱导沉默复合体)的蛋白复合物结合来影响靶基因的表达。虽然蛋白质复合物尚未完全表征,但人类EIF2C2蛋白已被确定为miRNP和RISC的一个组成部分。在本报告中,我们提出了针对EIF2C2的抗血清,以便开始了解蛋白质复合物。免疫印迹结果表明,EIF2C2蛋白在人类和小鼠的多种细胞系中普遍表达。EIF2C2蛋白存在于两个细胞区室中,通过HeLa S3裂解物的核提取物和细胞质部分(S100部分)的免疫印迹分析表明。EIF2C1或EIF2C2蛋白的缺失导致microRNA的减少,提示这些蛋白可能在microRNA的稳定性或生物发生中起作用。我们还制备了针对dsRNA结合蛋白PACT的抗血清,已知其在秀丽隐杆线虫和果蝇中的同源物在RNAi (RNA干扰)途径中起作用。PACT蛋白在多种细胞系中均有表达。
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引用次数: 1
Two semi‐subterranean copepods from Korea 来自韩国的两只半地下桡足动物
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647747
Ji Min Lee, Jin Mo Jeon, C. Chang
Two freshwater cyclopoid species including a new species are added to Korean copepod fauna: Ochridacyclops coreensis n. sp. and Itocyclops yezoensis (Ito, 1953). Both species were collected from the semi‐subterranean waters like springs and wells. The present paper deals with the description of the new species and the systematic accounts on the two species, based on the morphological character comparison with the related congeners.
在韩国桡足动物群中增加了两种淡水类,包括一新种:ochridyyclops coreensis n. sp.和Itocyclops yezoensis (Ito, 1953)。这两个物种都是从半地下的泉水和井中采集的。本文在与近缘同属植物形态学特征比较的基础上,对该新种进行了描述,并对两种进行了系统描述。
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引用次数: 5
Two new species of the genus Mycale (Naviculina) (Poecilosclerida: Mycalidae) from Ulleungdo Island, Korea 文章题目郁陵岛mycali (Naviculina)属二新种(水蛭目:Mycalidae)
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647736
C. Sim, D. Kang
A study on marine sponges was conducted by SCUBA diving at Ulleungdo Island, Korea, from July 2001 to August 2003. Two species, Mycale (Naviculina) ulleugensis and Mycale (N.) neunggulensis, are new to science. Mycale (N.) ulleugensis n. sp. seems close to M. chungae based on the shape of spicules, but differs in the size of megascleres and sigmas, Mycale (N.) neunggulensis n. sp. seems close to M. flagellifer Vacelet and Vasseur in the type and size of spicules, but differs in the shape of naviculichela. Naviculichela I of M. flagellifer has a spine on the upper part of the felix.
2001年7月至2003年8月,在郁陵岛用水肺潜水对海绵进行了研究。两种Mycale (Naviculina) ulleugensis和Mycale (N.) neunggulensis是科学上的新物种。Mycale (N.) ulleugensis N. sp.在针状体的形状上与M. chungae相似,但在大针状体和sigmas的大小上不同;Mycale (N.) neunggulensis N. sp.在针状体的类型和大小上与M. flagellifer Vacelet和Vasseur相似,但在naviculichela的形状上不同。鞭毛虫的Naviculichela I在肢肢上部有棘。
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引用次数: 2
Differential responses of large and small male red‐spotted newts, Notophthalmus viridescens, to conspecific chemical cues 大型和小型雄性红点蝾螈对同种化学信号的不同反应
Pub Date : 2004-01-01 DOI: 10.1080/12265071.2004.9647738
Daesik Park, J. McGuire, H. Eisthen
Male red‐spotted newts, Notophthalmus viridescens, use chemical cues to locate and select potential mates. To investigate whether large and small males respond differentially to chemical cues from conspecifics, we conducted a series of two‐choice Y‐maze tests with odorant stimuli from two large females (LF/LF), one large versus one small female (LF/SF), and one large female placed with four males versus one small female (LF+4M/SF). We found that males responded more rapidly in a LF/LF choice situation than in those involving LF/SF or LF+4M/SF. In addition, small males chose more quickly than did large males. These results suggest that both body size and odorant context affect the speed of odorant responses. By responding differentially to conspecific chemical cues, small males may arrive earlier at a courting area, increasing their chances of successfully guarding and mating with a female.
雄性红点蝾螈(Notophthalmus viri)利用化学线索来定位和选择潜在的配偶。为了研究大雄性和小雄性对同种生物的化学线索是否有不同的反应,我们进行了一系列双选项Y迷宫测试,其中包括两只大雌性(LF/LF)、一只大雌性对一只小雌性(LF/SF)以及一只大雌性与四只雄性对一只小雌性(LF+4M/SF)的气味刺激。我们发现,雄性在LF/LF选择情况下的反应比LF/SF或LF+4M/SF的反应更快。此外,体型小的雄性比体型大的雄性选择得更快。这些结果表明,体型和气味环境都会影响气味反应的速度。通过对同种化学物质的不同反应,体型较小的雄性可能会更早到达求偶区域,从而增加它们成功守护并与雌性交配的机会。
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引用次数: 1
期刊
Korean journal of biological sciences
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