Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647676
H. Ko, Hoi-Jeong Yang
The first zoeal stage of Pagurus japonicus is described and illustrated. Its morphological characteristics are compared with those of other known species of the genus Pagurus. The first zoea of P. japonicus was very similar to that of P. similis. The zoeas of the two species could be distinguished from other pagurid zoeas by morphological characteristics of the carapace without posterolateral spine, two setae on the endopod of the antenna, and 3+3 setae on the endopod of the maxilla. They show affinities more to the species of the family Diogenidae than to the species of the genus Pagurus in respect of zoeal morphology.
{"title":"First zoea of Pagurus japonicus (Crustacea: Decapoda: Anomura: Paguridae) reared in the laboratory","authors":"H. Ko, Hoi-Jeong Yang","doi":"10.1080/12265071.2003.9647676","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647676","url":null,"abstract":"The first zoeal stage of Pagurus japonicus is described and illustrated. Its morphological characteristics are compared with those of other known species of the genus Pagurus. The first zoea of P. japonicus was very similar to that of P. similis. The zoeas of the two species could be distinguished from other pagurid zoeas by morphological characteristics of the carapace without posterolateral spine, two setae on the endopod of the antenna, and 3+3 setae on the endopod of the maxilla. They show affinities more to the species of the family Diogenidae than to the species of the genus Pagurus in respect of zoeal morphology.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"11 - 14"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647678
C. Sim, Young A. Kim
Two new species of the genus Stelletta (Astrophorida: Ancorinidae), S. spinulosa n. sp. and S. calyx n. sp., are described from the waters around Gageodo Island (Sonuksando) and Ulleungdo Island, Korea. S. spinulosa n. sp. is similar to S. crassispicula (Sollas, 1886) in the feature of entire animal, but differs from it in the shape of orthotriaene and the form and size of oxyasters. S. calyx n. sp. resembles to S. crater Dendy, 1924 in the shape of sponge, but the new species has four size categories of oxyaster whereas S. carter has no oxyaster.
报道了韩国加岛岛(Sonuksando)和郁陵岛(ulledo Island)附近海域发现的两新种，即stellulosa n. sp和S. calyx n. sp。S. spinulosa n. sp.在整个动物的特征上与S. crassispicula (Sollas, 1886)相似，但在正三烯的形状和氧源的形状和大小上有所不同。S. calyx n. sp.在海绵形状上与S. crater Dendy, 1924相似，但新种有四种大小类别的氧虫，而S. carter没有氧虫。
{"title":"Two new sponge species of the genus Stelletta (Astrophorida: Ancorinidae) from Korea","authors":"C. Sim, Young A. Kim","doi":"10.1080/12265071.2003.9647678","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647678","url":null,"abstract":"Two new species of the genus Stelletta (Astrophorida: Ancorinidae), S. spinulosa n. sp. and S. calyx n. sp., are described from the waters around Gageodo Island (Sonuksando) and Ulleungdo Island, Korea. S. spinulosa n. sp. is similar to S. crassispicula (Sollas, 1886) in the feature of entire animal, but differs from it in the shape of orthotriaene and the form and size of oxyasters. S. calyx n. sp. resembles to S. crater Dendy, 1924 in the shape of sponge, but the new species has four size categories of oxyaster whereas S. carter has no oxyaster.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"25 - 28"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647680
M. Moon
The principal fibers used in constructing the cocoon in the garden spider, Argiope aurantia, are large‐diameter fibers developed from tubuliform glands and small‐diameter fibers presumed to be spun by the aciniform silk glands. Scanning electron micrographs of the large‐diameter fibers on both surfaces of the cocoon clearly reveal their fine structural differences. While the silk fibers on the inner surface have smooth and homogeneous appearances, each fiber on the outer surface represents a multicomponent internal structure. Examination of each fibers using transmission electron microscope also provides additional evidence that the multicomponent fibers contain numerous electron lucent fibrils embedded in an amorphous electron dense matrix. It has been also revealed that two types of secretory granules presumed to be the precursors of tubuliform fibers are closely related to the production of distinct coloration in luminal contents ‐ brownish and yellowish components. Moreover, these electron‐dense granules, possibly precursor of fibrillar component, and electron‐lucent granules, possibly precursor of matrix component, are densely packed and remain close to each other without fusion. It is critical evidence that the individual tubuliform fiber is not only heterogeneous and multicomponent but also takes place in a variety at manners throughout the length of the gland.
{"title":"Fine structural analysis of the cocoon silk production in the garden spider, Argiope aurantia","authors":"M. Moon","doi":"10.1080/12265071.2003.9647680","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647680","url":null,"abstract":"The principal fibers used in constructing the cocoon in the garden spider, Argiope aurantia, are large‐diameter fibers developed from tubuliform glands and small‐diameter fibers presumed to be spun by the aciniform silk glands. Scanning electron micrographs of the large‐diameter fibers on both surfaces of the cocoon clearly reveal their fine structural differences. While the silk fibers on the inner surface have smooth and homogeneous appearances, each fiber on the outer surface represents a multicomponent internal structure. Examination of each fibers using transmission electron microscope also provides additional evidence that the multicomponent fibers contain numerous electron lucent fibrils embedded in an amorphous electron dense matrix. It has been also revealed that two types of secretory granules presumed to be the precursors of tubuliform fibers are closely related to the production of distinct coloration in luminal contents ‐ brownish and yellowish components. Moreover, these electron‐dense granules, possibly precursor of fibrillar component, and electron‐lucent granules, possibly precursor of matrix component, are densely packed and remain close to each other without fusion. It is critical evidence that the individual tubuliform fiber is not only heterogeneous and multicomponent but also takes place in a variety at manners throughout the length of the gland.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"35 - 41"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647680","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647718
Sangkyu Park, Kang-Hyun Cho
To quantify nutrient loading from emergent macrophytes through leaching in the littoral zones of Paldang Reservoir, we conducted incubation experiments using leaf litter of the emergent macrophyte, Zizania latifolia. To separate the leaching process from microbial decay, we used HgCl2 to suppress microbial activity during the experiment. We measured electric conductivity, absorbance at 280 nm, total nitrogen and dissolved inorganic nitrogen, total phosphorus and soluble reactive phosphorus, Na, K, Mg and Ca amounts in leaf litter and in water. In addition, we examined the effects of water temperature and ion concentrations of ambient water on the leaching process. A total of 6% of the initial ash‐ free dry mass of leaf litter was lost due to leaching during incubation (four days). Electric conductivity and A280 continued to increase and saturate during the incubation. To compare reaching rates of different nutrients, we fitted leaching dynamics with a hyperbolic saturation function [Y=A•X/(B+X)]. From these fittings, we found that ratios of leaching amounts to nutrient concentration in the litter were in the order of K>Na>Mg>P>Ca>N. Leaching from leaf litter of Z. latifolia was dependent on water temperature while it was not related with ion concentrations in the ambient water. Our results suggest that the leaching process of nutrients, especially phosphorus, from aquatic macrophytes provides considerable contribution to the eutrophication of the Paldang Reservoir ecosystem.
{"title":"Nutrient leaching from leaf litter of emergent macrophyte (Zizania latifolia) and the effects of water temperature on the leaching process","authors":"Sangkyu Park, Kang-Hyun Cho","doi":"10.1080/12265071.2003.9647718","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647718","url":null,"abstract":"To quantify nutrient loading from emergent macrophytes through leaching in the littoral zones of Paldang Reservoir, we conducted incubation experiments using leaf litter of the emergent macrophyte, Zizania latifolia. To separate the leaching process from microbial decay, we used HgCl2 to suppress microbial activity during the experiment. We measured electric conductivity, absorbance at 280 nm, total nitrogen and dissolved inorganic nitrogen, total phosphorus and soluble reactive phosphorus, Na, K, Mg and Ca amounts in leaf litter and in water. In addition, we examined the effects of water temperature and ion concentrations of ambient water on the leaching process. A total of 6% of the initial ash‐ free dry mass of leaf litter was lost due to leaching during incubation (four days). Electric conductivity and A280 continued to increase and saturate during the incubation. To compare reaching rates of different nutrients, we fitted leaching dynamics with a hyperbolic saturation function [Y=A•X/(B+X)]. From these fittings, we found that ratios of leaching amounts to nutrient concentration in the litter were in the order of K>Na>Mg>P>Ca>N. Leaching from leaf litter of Z. latifolia was dependent on water temperature while it was not related with ion concentrations in the ambient water. Our results suggest that the leaching process of nutrients, especially phosphorus, from aquatic macrophytes provides considerable contribution to the eutrophication of the Paldang Reservoir ecosystem.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"289 - 294"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647708
G. Kim
The tadpole larva of the ascidian Halocynthia roretzi has two sensory pigment cells in its brain vesicle. To elucidate the temporal requirement for FGF signaling in formation of the pigment cells, embryos were treated with an FGF receptor 1 inhibitor, SU5402, or an MEK inhibitor, U0126 during various embryonic stages. In the present study, it is shown that the embryos treated with SU5402 from the 16‐cell stage to the early gastrula stage do not form pigment cells, whereas those treated after the early gastrula stage form pigment cells. In pigment cell formation, embryos suddenly exhibited the sensitivity to SU5402 only for 1 h at the neural plate stage (∼4 h after the beginning of gastrulation). When U0126 treatment was carried out at various stages between the 8‐cell and late neurula stages, the embryos scarcely formed pigment cells. Pigment cell formation occurred when the embryos were placed in U0126 at early tailbud stage. These results indicate that FGF signaling is involved in pigment cell formation at two separate processes during ascidian embryogenesis, whereas more prolonged period is required for MEK signaling.
{"title":"Formation of sensory pigment cells requires fibroblast growth factor signaling during ascidian embryonic development","authors":"G. Kim","doi":"10.1080/12265071.2003.9647708","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647708","url":null,"abstract":"The tadpole larva of the ascidian Halocynthia roretzi has two sensory pigment cells in its brain vesicle. To elucidate the temporal requirement for FGF signaling in formation of the pigment cells, embryos were treated with an FGF receptor 1 inhibitor, SU5402, or an MEK inhibitor, U0126 during various embryonic stages. In the present study, it is shown that the embryos treated with SU5402 from the 16‐cell stage to the early gastrula stage do not form pigment cells, whereas those treated after the early gastrula stage form pigment cells. In pigment cell formation, embryos suddenly exhibited the sensitivity to SU5402 only for 1 h at the neural plate stage (∼4 h after the beginning of gastrulation). When U0126 treatment was carried out at various stages between the 8‐cell and late neurula stages, the embryos scarcely formed pigment cells. Pigment cell formation occurred when the embryos were placed in U0126 at early tailbud stage. These results indicate that FGF signaling is involved in pigment cell formation at two separate processes during ascidian embryogenesis, whereas more prolonged period is required for MEK signaling.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"221 - 225"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647697
B. Kang, N. Ha
Adult periodontitis is a chronic inflammatory disease whose etiology is not well defined. Recent studies have shown that vitamin D receptor gene has been a candidate for the susceptibility of adult periodontitis. The purpose of this study is to investigate the frequency of Taq I restriction fragment length polymorphism (RFLP) in the vitamin D receptor gene in 141 periodontically healthy controls and 28 adult periodontitis patients. Taq I RFLP in the vitamin D receptor gene was detected by PCR amplification, followed by restriction enzyme digestion and 2% agarose gel electrophoresis. There was no significant difference in the distribution of Taq I RFLP between healthy controls and adult periodontitis group (P > 0.05). Thus, Taq I RFLP in the vitamin D receptor gene may not confer the susceptibility to adult periodontitis in Korean population. However, t allele distributions of this RFLP showed various frequencies among ethnic groups studied. Further studies in other ethnic groups will be required.
{"title":"Association study between vitamin D receptor gene polymorphism and adult periodontitis in Korean","authors":"B. Kang, N. Ha","doi":"10.1080/12265071.2003.9647697","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647697","url":null,"abstract":"Adult periodontitis is a chronic inflammatory disease whose etiology is not well defined. Recent studies have shown that vitamin D receptor gene has been a candidate for the susceptibility of adult periodontitis. The purpose of this study is to investigate the frequency of Taq I restriction fragment length polymorphism (RFLP) in the vitamin D receptor gene in 141 periodontically healthy controls and 28 adult periodontitis patients. Taq I RFLP in the vitamin D receptor gene was detected by PCR amplification, followed by restriction enzyme digestion and 2% agarose gel electrophoresis. There was no significant difference in the distribution of Taq I RFLP between healthy controls and adult periodontitis group (P > 0.05). Thus, Taq I RFLP in the vitamin D receptor gene may not confer the susceptibility to adult periodontitis in Korean population. However, t allele distributions of this RFLP showed various frequencies among ethnic groups studied. Further studies in other ethnic groups will be required.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"12 1","pages":"145 - 149"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647707
Hong Nam Kim, J. Cha, T. Cho, Hak Yong Kim
The response of plants to pathogens and wounding is dependent upon very sensitive perception mechanisms. Although genetic approaches have revealed a variety of resistance genes that activate common defense responses, defense‐related proteins are not well characterized in plants. Therefore, we used a proteomic approach to determine which defense‐related proteins are induced by salicylic acid (SA) and wounding in Chinese cabbage. We found that SA and wounding induce pathogenesis‐related protein 1a (PR1a) at both protein and mRNA levels using proteomics and Northern blot analysis, respectively. This indicates that our proteomic approach is useful for identifying defense‐related proteins. We also identified several other proteins that are induced by SA or wounding. Among the seven SA‐induced proteins identified, four may be defense‐related, including defense‐related protein, phospholipase D (PLD), resistance protein RPS2 homolog, and L‐ascorbate peroxidase. Out of the six wounding‐induced proteins identified, three may be defense‐related: heat shock cognate protein 70 (HSC70), polygalacturonase, and peroxidase P7. The precise functions of these proteins in plant defense responses await further study. However, identification of the defense‐related proteins described in this study should allow us to better understand the mechanisms and signal transduction pathways involved in defense responses in Chinese cabbage.
{"title":"Salicylic acid and wounding induce defense‐related proteins in Chinese cabbage","authors":"Hong Nam Kim, J. Cha, T. Cho, Hak Yong Kim","doi":"10.1080/12265071.2003.9647707","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647707","url":null,"abstract":"The response of plants to pathogens and wounding is dependent upon very sensitive perception mechanisms. Although genetic approaches have revealed a variety of resistance genes that activate common defense responses, defense‐related proteins are not well characterized in plants. Therefore, we used a proteomic approach to determine which defense‐related proteins are induced by salicylic acid (SA) and wounding in Chinese cabbage. We found that SA and wounding induce pathogenesis‐related protein 1a (PR1a) at both protein and mRNA levels using proteomics and Northern blot analysis, respectively. This indicates that our proteomic approach is useful for identifying defense‐related proteins. We also identified several other proteins that are induced by SA or wounding. Among the seven SA‐induced proteins identified, four may be defense‐related, including defense‐related protein, phospholipase D (PLD), resistance protein RPS2 homolog, and L‐ascorbate peroxidase. Out of the six wounding‐induced proteins identified, three may be defense‐related: heat shock cognate protein 70 (HSC70), polygalacturonase, and peroxidase P7. The precise functions of these proteins in plant defense responses await further study. However, identification of the defense‐related proteins described in this study should allow us to better understand the mechanisms and signal transduction pathways involved in defense responses in Chinese cabbage.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"213 - 219"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647709
Chang-Beom Park, Byung Ho Kim, O. Na, Y. Choi, Young don Lee, H. Baek, Hyung Bae Kim, A. Takemura
Bisphenol A (BPA), nonylphenol (NP), and 4‐tert‐octylphenol (OP) are known endocrine disrupting chemicals (EDCs) with estrogenic activity in fish. This study compared the effects of BPA, NP and OP on in vitro vitellogenin (VTG) synthesis in primary cultures of hepatocytes of the Chinese minnow Phoxinus oxycephalus. The VTG secreted into the culture medium was measured using enzyme‐linked immunosorbent assay (ELISA), which we developed in this study using an antibody prepared from homogenates of Chinese minnow egg. VTG synthesis was induced by estradiol‐17β (E2) and phenols (BPA, NP and OP) treatment. E2 at concentrations of 10‐6 M or higher increased VTG levels significantly (P<0.05). Exposure to 10‐5 M BPA or 10‐4 M NP and OP induced in vitro VTG synthesis (P<0.01). However, 10‐3 M BPA, NP or OP did not induce VTG synthesis. These results suggest that BPA has the highest estrogenic potential in Chinese minnow hepatocytes. Tamoxifen, an anti‐estrogen, drastically blocked the production of VTG by phenols (BPA, NP and OP) suggesting that phenols (BPA, NP and OP) may act via binding to estrogen receptor (ER) in Chinese minnow hepatocytes.
双酚A (BPA)、壬基酚(NP)和4 -叔辛基酚(OP)是已知的内分泌干扰物质(EDCs)，在鱼类中具有雌激素活性。本研究比较了BPA、NP和OP对中国鲦鱼(Phoxinus oxycephalus)肝细胞原代培养体外卵黄蛋白原蛋白(vitellogenin, VTG)合成的影响。利用酶联免疫吸附法(ELISA)检测分泌到培养基中的VTG，该方法是我们利用从中国鲦鱼蛋匀浆中制备的抗体开发的。雌二醇- 17β (E2)和酚类(BPA、NP和OP)处理诱导VTG合成。E2浓度为10‐6 M或更高时，VTG水平显著升高(P<0.05)。暴露于10‐5 M BPA或10‐4 M NP和OP诱导体外VTG合成(P<0.01)。然而，10‐3 M BPA、NP或OP不诱导VTG合成。这些结果表明BPA在中国鲦鱼肝细胞中具有最高的雌激素潜能。他莫昔芬是一种抗雌激素，可显著阻断酚类物质(BPA、NP和OP)对VTG的产生，这表明酚类物质(BPA、NP和OP)可能通过与中国鲦鱼肝细胞雌激素受体(ER)结合而起作用。
{"title":"Induction of in vitro vitellogenin synthesis by bisphenol, nonylphenol and octylphenol in Chinese minnow (phoxinus oxycephalus) hepatocytes","authors":"Chang-Beom Park, Byung Ho Kim, O. Na, Y. Choi, Young don Lee, H. Baek, Hyung Bae Kim, A. Takemura","doi":"10.1080/12265071.2003.9647709","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647709","url":null,"abstract":"Bisphenol A (BPA), nonylphenol (NP), and 4‐tert‐octylphenol (OP) are known endocrine disrupting chemicals (EDCs) with estrogenic activity in fish. This study compared the effects of BPA, NP and OP on in vitro vitellogenin (VTG) synthesis in primary cultures of hepatocytes of the Chinese minnow Phoxinus oxycephalus. The VTG secreted into the culture medium was measured using enzyme‐linked immunosorbent assay (ELISA), which we developed in this study using an antibody prepared from homogenates of Chinese minnow egg. VTG synthesis was induced by estradiol‐17β (E2) and phenols (BPA, NP and OP) treatment. E2 at concentrations of 10‐6 M or higher increased VTG levels significantly (P<0.05). Exposure to 10‐5 M BPA or 10‐4 M NP and OP induced in vitro VTG synthesis (P<0.01). However, 10‐3 M BPA, NP or OP did not induce VTG synthesis. These results suggest that BPA has the highest estrogenic potential in Chinese minnow hepatocytes. Tamoxifen, an anti‐estrogen, drastically blocked the production of VTG by phenols (BPA, NP and OP) suggesting that phenols (BPA, NP and OP) may act via binding to estrogen receptor (ER) in Chinese minnow hepatocytes.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"227 - 235"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647712
Seung-Ho Hong
Mutations in the factor V gene are major risk markers for venous thrombosis. Several factors for blood coagulation have been related with cardiovascular disease. I investigated genotype distribution for three mutations (G1691A, A2379G and G2391 A) of the factor V gene in the Korean population. Genotype frequencies were examined by polymerase chain reaction in 135 patients with coronary artery disease (CAD) and 116 healthy subjects. For the G1691A mutation (factor V Leiden), no mutation was detected in either group. Allele frequencies of A2379G and G2391A mutations were not significantly different between CAD patients and controls. Non‐Caucasian populations have a considerably lower factor V Leiden allele frequency than Caucasian populations. Thus, it may be due to differences in the genetic background as well as environmental factors.
{"title":"Genotype distribution of the mutations in the coagulation factor V gene in the Korean population: Absence of its association with coronary artery disease","authors":"Seung-Ho Hong","doi":"10.1080/12265071.2003.9647712","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647712","url":null,"abstract":"Mutations in the factor V gene are major risk markers for venous thrombosis. Several factors for blood coagulation have been related with cardiovascular disease. I investigated genotype distribution for three mutations (G1691A, A2379G and G2391 A) of the factor V gene in the Korean population. Genotype frequencies were examined by polymerase chain reaction in 135 patients with coronary artery disease (CAD) and 116 healthy subjects. For the G1691A mutation (factor V Leiden), no mutation was detected in either group. Allele frequencies of A2379G and G2391A mutations were not significantly different between CAD patients and controls. Non‐Caucasian populations have a considerably lower factor V Leiden allele frequency than Caucasian populations. Thus, it may be due to differences in the genetic background as well as environmental factors.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"255 - 259"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647713
Jin Kyeoung Kim, D. Oh, S. Kwak, Jingjun Han, Y. Chung, N. Kim
Vascular endothelial growth factor (VEGF) is a potent regulator of normal and abnormal angiogenesis. Recent literature suggests that VEGF has several activities that may amplify acute inflammation reactions. Dysregulated VEGF expression has been implicated as a major contributor to the development of a number of common disease pathologies. One of common mutations in the 3'‐untranslated region of the VEGF gene, a C?T exchange at nucleotide position 936, has been found to be significantly associated with VEGF expression levels in the plasma from a previous Austrian study. The frequency of this mutation could be important genetic information regarding tumor growth and angiogenesis related diseases. The aim of this study was to investigate the frequency distribution of this mutation in general Korean population. We examined the statistical data from 207 healthy Korean subjects. Observed numbers (%) of 936T were 28.5 (CT) and 3.9 (TT), respectively. The mutant allele frequency of 936T in Korean subjects was 0.18, which appeared somewhat higher than that in Austrian subjects.
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