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Photothermal Quotient Describes the Combined Effects of Heat and Shade Stresses on Canola Seed Productivity 光热商描述了热阴胁迫对油菜籽产量的综合影响
Pub Date : 2023-03-08 DOI: 10.3390/seeds2010012
G. M. Rivelli, N. V. Gómez, A. Mantese, D. Miralles, L. Abeledo, Deborah Paola Rondanini
There is evidence of the negative effects on canola seed yield caused by shading (SH) and high temperature stress (HT) separately, but the combined effect of both stresses has not been studied. This work aimed to (i) evaluate the effects of SH and HT stresses, alone and combined, on floral development, seed yield and quality, (ii) quantify the resulting effect (additive, synergistic, antagonistic) of combined stresses, and (iii) examine the utility of the photothermal quotient (PTQ, solar radiation/temperature ratio) to predict seed yield in stressed canola crops. Two field experiments were performed in Buenos Aires (Argentina) applying HT daytime temperature stress (25–30 °C from 1000 to 1500 h), SH (−80% irradiance), and SH + HT combined stresses, with C unstressed (20 °C and 100% irradiance) crops. Long and short duration SH and HT strongly affected floral development (fewer flowers and pods, with smaller ovules) and seed yield (reduction from −40 to −90% respect to C). Combined SH + HT exhibited detrimental synergistic effects on seed yield and oil concentration for long duration stresses, whereas antagonistic effects were mainly observed for short stresses. We conclude that the PTQ (cumulative from 100 to 500 °Cd after flowering) summarizes adequately the detrimental effects of combined post-flowering abiotic stresses on canola seed productivity.
有证据表明,遮荫和高温胁迫分别对油菜籽产量产生负面影响,但两种胁迫的联合效应尚未得到研究。本研究旨在(i)评估单用和复合胁迫对花发育、种子产量和品质的影响,(ii)量化复合胁迫的效应(加性、增效、拮抗),以及(iii)检验光热商(PTQ,太阳辐射/温度比)在预测受胁迫油菜籽作物种子产量方面的应用。在阿根廷布宜诺斯艾利斯进行了两项田间试验,分别采用高温日间温度胁迫(25-30°C, 1000 ~ 1500 h)、高温(- 80%辐照度)和高温+高温联合胁迫,以及无高温胁迫(20°C, 100%辐照度)作物。长时间和短时间的高温胁迫和高温胁迫对花的发育(花和荚减少,胚珠变小)和种子产量(相对于C降低- 40%到- 90%)都有强烈的影响。在长时间胁迫下,高温胁迫和高温胁迫联合对种子产量和油浓度有不利的协同效应,而在短时间胁迫下主要观察到拮抗作用。我们认为,PTQ(开花后100 ~ 500°Cd的累积值)充分总结了花后非生物胁迫对油菜种子生产力的不利影响。
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引用次数: 2
Field Assessment of Two Micronutrients (Zinc and Boron) on the Seed Yield and Oil Content of Mustard 两种微量元素(锌和硼)对芥菜籽粒产量和含油量的田间评价
Pub Date : 2023-03-01 DOI: 10.3390/seeds2010010
A. Halim, S. Paul, M. A. R. Sarkar, Md. Harun Ar Rashid, S. Perveen, Md. Liton Mia, M. Islam, A. Islam
In an experimental investigation, we looked into how different zinc (Zn) and boron (B) dosages affected the production and the amount of oil in mustard. Zn and B treatments, respectively, were separated into four levels: 0, 1, 2, 4, and 0, 0.4, 0.6, and 0.8 kg per hectare. We observed considerable Zn as well as B effects on mustard yield and its oil content. The maximum (1.6 Ton per hectare) and minimum (1.3 T ha−1) seed output, the maximum (1.9 T ha−1) and minimum (1.5 T ha−1) stover production, the maximum (4.9) and minimum (3.99) number branches plant−1, and the longest (114.6 cm) and shortest (87.44 cm) plant height were observed from 4 kg of Zn ha−1 and 0 kg of Zn ha−1, respectively. On the other hand, we discovered that applying 0.8 kg and 0 kg of B ha−1, respectively, resulted in the highest (1.6 T ha−1) and lowest (1.3 T ha−1) seed yield, the highest (1.8 T ha−1) and lowest (1.5 T ha−1) stover yield, the maximum (4.75) and minimum (4.02) number of branches plant−1, and the longest (118.7 cm) and shortest (85.15 cm) plant heights. The maximum seed (1.9 T ha−1) and stover output (2.0 T ha−1), tallest plant (140.9 cm), and most branches per plant−1 (5.47) were obtained when 4 kg of Zn ha−1 was given with 0.8 kg of B ha−1. However, while Zn and B were not applied, the lowest output for all of the crop attributes assessed was observed. As a result, for mustard, 4 kg of Zn ha−1 combined with 0.8 kg of B ha−1 may be advised as an effective approach in terms of seed yield and oil content.
在实验研究中,我们研究了不同锌(Zn)和硼(B)用量对芥菜产量和油量的影响。Zn和B处理分别分为0、1、2、4和0、0.4、0.6和0.8 kg /公顷4个水平。我们观察到锌和B对芥菜产量和含油量有相当大的影响。在4 kg Zn ha - 1和0 kg Zn ha - 1处理下,种子产量最高(1.6 T /公顷)和最小(1.3 T /公顷),秸秆产量最高(1.9 T /公顷)和最小(1.5 T /公顷),单株分枝数最高(4.9)和最小(3.99),株高最高(114.6 cm)和最短(87.44 cm)。另一方面,我们发现,分别施用0.8 kg和0 kg B ha - 1,种子产量最高(1.6 T ha - 1)和最低(1.3 T ha - 1),秸秆产量最高(1.8 T ha - 1)和最低(1.5 T ha - 1),枝数最高(4.75)和最小(4.02),株高最高(118.7 cm)和最短(85.15 cm)。当4 kg Zn + 0.8 kg B处理时,种子最多(1.9 T ha−1),秸秆产量最高(2.0 T ha−1),植株最高(140.9 cm),单株分枝数最多(5.47)。然而,当不施用锌和B时,观察到所有作物属性的最低产量。综上所述,就芥菜种子产量和含油量而言,4 kg Zn + 0.8 kg bha - 1可能是一种有效的处理方式。
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引用次数: 2
Dormancy Breaking of Teramnus labialis (L.f.) Spreng Seeds Is Affected by the Extent of Liquid Nitrogen Exposure 唇斑田鼠(L.f)的休眠解除液氮暴露程度对春种子的影响
Pub Date : 2023-03-01 DOI: 10.3390/seeds2010011
Yanier Acosta Fernández, Lianny Pérez Gómez, Dayamí Fontes Marrero, Marcos Edel Martínez-Montero
Teramnus labialis (L.f.) Spreng shows dormancy as a result of impermeability of the seed coat, which requires scarification treatment before sowing. Liquid nitrogen (LN) as a scarifying treatment has recently been used on this species, with excellent results. However, moisture content and immersion time on LN are factors that may affect seed germination and dormancy break. This report studies (i) the effects of dehydration on T. labialis seed viability and germination and (ii) the appropriate moisture content and extent of LN to make this scarification an effective treatment. Moisture contents of 4%, 6%, 8%, and 10% fresh weight basis (FWB) and extension to LN for 15, 30, 45, and 60 min were performed. Seed viability did not change after seed dehydration up to a moisture content of 4% FWB, whereas the percentage of germination decreased as a result of increasing the percentage of hard seeds. The seed moisture content did not affect germination after immersion in LN, but at least 30 min of exposure was required for dormancy break. The mean germination time, germination index, and time to 50% germination improved with the increasing germination from 33% to 91% and a greater duration of immersion in LN. The dormancy of T. labialis seeds with a moisture content between 4% and 10% FWB was only broken when exposed to LN for 30 to 60 min.
唇足(l.f.)由于种皮不透水,春季表现出休眠状态,这需要在播种前进行切割处理。液氮(LN)作为一种疤痕处理最近被用于该物种,具有良好的效果。而LN上的含水率和浸泡时间是影响种子萌发和休眠的主要因素。本报告研究了(i)脱水对唇瓢虫种子活力和萌发的影响,(ii)适当的水分含量和LN的程度,使这种割伤成为一种有效的处理。水分含量分别为4%、6%、8%和10%的鲜重(FWB),并延长至LN 15、30、45和60分钟。在水分含量达到4% FWB时,种子脱水后种子活力没有变化,但随着硬种子比例的增加,种子发芽率下降。浸渍后种子含水量不影响萌发,但至少需要30min才能休眠。随着发芽率从33%增加到91%,浸渍时间延长,平均发芽时间、发芽指数和50%发芽率均有所提高。水分含量在4% ~ 10% FWB之间的唇形瓢虫种子,在LN作用30 ~ 60 min后休眠才被打破。
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引用次数: 0
Evaluation of Training on Good Agronomic Practices for Seed Yam Production in Nigeria 尼日利亚种子山药生产良好农艺规范培训评价
Pub Date : 2023-02-28 DOI: 10.3390/seeds2010009
D. Mignouna, B. Aighewi, N. Maroya, B. Akinribido, M. Balogun, O. T. Akintayo, D. Amah, B. Awotide, P. D. Dontsop Nguezet, T. Abdoulaye, R. Asiedu, V. Manyong
The second phase of the Yam Improvement for Income and Food Security in West Africa (YIIFSWA II) project was implemented through a strong collaboration between research and private sector. Private seed companies were provided with a wide variety of training and development activities on good agronomic practices (GAPs). However, the effects of the training activities were yet to be evaluated. This paper evaluated the contribution of the training on high-quality seed yam production through paying particular attention to how it was tailored to the needs of various farmers, and how the knowledge gained was transferred to the field. The study employed an applied Kirkpatrick model and descriptive statistics to measure the indicators of training effectiveness. Results from a statistical population of 172 farmers using 179 demonstration fields showed that the farmers’ training on GAPs was effective, based on four criteria of the Kirkpatrick training evaluation process. The satisfactory results at the beginning of the hierarchical model have implications on the satisfactory results at the end. Improving the effectiveness of a training depends on defining factors such as (i) Training based on satisfaction in meeting the needs of your trainees and the seed production business; (ii) Training based on learning objectives in improving the trainees’ skills and knowledge; and (iii) Training based on learner attitude change in applying new skills and knowledge in the fields. Conclusions drawn from this study indicated that the training was a reasonable value-added mechanism of improved agricultural knowledge and practices for enhancing high-quality seed yam production in Nigeria.
西非改善薯类收入和粮食安全项目第二阶段是通过研究部门与私营部门之间的强有力合作实施的。向私营种子公司提供了各种关于良好农艺做法的培训和发展活动。但是,培训活动的效果还有待评价。本文通过特别关注如何根据不同农民的需求进行培训,以及如何将所获得的知识转移到田间,评估了高质量山药种子生产培训的贡献。本研究采用应用Kirkpatrick模型和描述性统计对培训效果指标进行测量。基于Kirkpatrick培训评估过程的四个标准,对179个示范田172名农民的统计结果表明,农民对gap的培训是有效的。层次模型一开始的满意结果会影响到最后的满意结果。提高培训的有效性取决于确定以下因素:(i)以满足培训人员和种子生产业务的需求为基础的培训;根据学习目标进行培训,以提高受训者的技能和知识;(三)根据学习者在各领域应用新技能和知识方面态度的改变进行培训。本研究的结论表明,培训是提高尼日利亚优质山药种子生产的农业知识和实践的合理增值机制。
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引用次数: 0
Improvement of Seed Quality by Priming: Concept and Biological Basis 引种改良种子品质:概念与生物学基础
Pub Date : 2023-02-20 DOI: 10.3390/seeds2010008
F. Corbineau, Nesrin Taskiran-Özbingöl, Hayat El-Maarouf-Bouteau
Presoaking seeds in water (hydropriming) or in a solution, usually of polyethylene glycol (PEG) or various salts at low water potential (osmopriming), has been demonstrated to improve the germination of seeds of numerous species including vegetables (carrot, celery, leek, lettuce, tomato), floral plants (cyclamen, primrose, pansy) and others (sugar beet, rape, soybean, sunflower). This treatment allows the germination stricto sensu to occur but prevents the radicle protrusion. Germination of primed seeds is more rapid and uniform than that of unprimed ones. Primed seeds germinate in a wider range of temperatures and are less sensitive to oxygen deprivation. Interestingly, priming also improves the germination of aged seeds. The stimulatory effect of priming persists after redrying and often during storage; however, primed seeds often deteriorate faster during storage or accelerated aging than unprimed ones. A better understanding of the mechanisms involved during priming allows us to suggest markers of the effectiveness of priming. Among these markers, ethylene production during imbibition, cell-cycle processes (DNA replication, ß-tubulin), soluble sugar metabolism (raffinose family oligosaccharides, in particular), reactive oxygen species scavenging through antioxidant systems and energy metabolism are correlated to seed vigor. Global approaches (proteomic, metabolomic or transcriptomic) could also result in the identification of new markers.
将种子浸泡在水中(水浸)或通常由聚乙二醇(PEG)或各种低水势盐组成的溶液中(水浸),已被证明可以提高许多物种种子的萌发,包括蔬菜(胡萝卜、芹菜、韭菜、生菜、番茄)、花卉植物(仙仙花、报春花、三色堇)和其他植物(甜菜、油菜、大豆、向日葵)。这种处理可以使发芽产生严格感,但防止胚根突出。处理后的种子萌发比未处理的种子更快、更均匀。浸渍过的种子在更大的温度范围内发芽,对缺氧不那么敏感。有趣的是,催熟还能促进陈年种子的萌发。再干燥后和储存期间,启动的刺激作用仍然存在;然而,与未处理的种子相比,处理过的种子在储存或加速老化过程中往往变质得更快。更好地理解启动过程中涉及的机制使我们能够提出启动有效性的标记。在这些标记中,吸胀过程中的乙烯产生、细胞周期过程(DNA复制、ß-微管蛋白)、可溶性糖代谢(特别是棉子糖家族低聚糖)、通过抗氧化系统清除活性氧和能量代谢与种子活力有关。全局方法(蛋白质组学、代谢组学或转录组学)也可能导致新标记的鉴定。
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引用次数: 0
Comparative Study of Cis- and Trans-Priming Effect of PEG and BABA in Cowpea Seedlings on Exposure to PEG-Induced Osmotic Stress PEG和BABA在豇豆幼苗中对PEG诱导的渗透胁迫顺式和反式启动效应的比较研究
Pub Date : 2023-02-13 DOI: 10.3390/seeds2010007
E. Soltani, K. Aswathi, A. Sen, J. T. Puthur
The growth and performance of cowpea seedlings are negatively impacted by climate change and the subsequent occurrence of drought stress. Osmotic stress leads to the formation of reactive oxygen species, causing membrane breakdown, and impairs metabolic activities. The harmful effects of osmotic stress can be reduced by using seed priming techniques. Seeds of cowpea var. Anaswara were treated with polyethylene glycol (PEG) and β-amino butyric acid (BABA) as priming agents. The seedlings emerged from the primed seeds have been found to reduce the lipid peroxidation rates and improve plant water status by accumulating osmolytes such as proline, total free aminoacids, and total soluble sugars, and also enhanced the production of non-enzymatic antioxidants such as total phenolics, ascorbate, and glutathione, as well as increased the activities of enzymatic antioxidants such as catalase, peroxidase, and superoxide dismutase, which effectively scavenge ROS and maintain the homeostasis of the cell. PEG priming (cis-priming) and BABA priming (trans-priming) exhibited differential physiochemical responses in cowpea subjected to PEG stress. The current work investigates the extent of stress tolerance acquired through seed priming, and it will help to make a sensitive variety to a more tolerant one. Physiochemical responses of seedlings emerged from BABA-primed seeds towards PEG stress were better regulated to encounter the PEG-induced osmotic stress than the seedlings emerged from PEG-primed seeds.
气候变化和随后发生的干旱胁迫对豇豆幼苗的生长和性能产生负面影响。渗透胁迫导致活性氧的形成,导致膜破裂,并损害代谢活动。利用种子引种技术可以减少渗透胁迫的有害影响。以聚乙二醇(PEG)和β-氨基丁酸(BABA)为引物,对豇豆品种Anaswara种子进行了处理。通过积累脯氨酸、总游离氨基酸和总可溶性糖等渗透性物质,研究人员发现,从引种种子中生长出来的幼苗降低了脂质过氧化率,改善了植物水分状况,还增加了总酚类、抗坏血酸和谷胱甘肽等非酶促抗氧化剂的产生,并增加了过氧化氢酶、过氧化物酶和超氧化物歧化酶等酶促抗氧化剂的活性。能有效清除活性氧,维持细胞内稳态。PEG启动(顺式启动)和BABA启动(反式启动)在PEG胁迫下表现出不同的理化反应。目前的工作是研究通过种子激发获得的抗逆性程度,这将有助于将敏感品种培育成更耐受性的品种。与PEG诱导的渗透胁迫相比,baba -引物种子对PEG胁迫的生理化学反应受到更好的调控。
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引用次数: 1
Enhancing the Yield Potential of Soybean after Magneto-Priming: Detailed Study on Its Relation to Underlying Physiological Processes 磁激发后大豆增产潜力及其与潜在生理过程关系的详细研究
Pub Date : 2023-02-10 DOI: 10.3390/seeds2010006
Juhie Joshi-Paneri, Sonika Sharma, K. Guruprasad, S. Kataria
Soybean (Glycine max) is one of the most important proteins and oilseed crops in the world due to a boom in its demand. In order to meet this demand, various modern agricultural methods are being employed, of which magneto-priming (treatment of seeds with magnetic field) is becoming the most popular technique owing to its efficiency and eco-friendly nature to improve seed vigour, growth and yield of soybean plants. Therefore, we conducted a field experiment to evaluate the impact of magneto-priming of seeds with static magnetic field on soybean var. JS-335 plants. We used static magnetic field (SMF) strengths of 150 mT (1 h) and 200 mT (1 h) for this study. Both the SMF treatments improved growth (shoot as well as root growth parameters), carbon fixation (PSII efficiency, gas exchange parameters, carbonic anhydrase activity) and nitrogen fixation (leghemoglobin content, total protein content, nitrate reductase activity). We observed an association between these parameters which contributed to biomass accumulation and hence to the enhanced crop yield. In addition, reduced levels of ASA (reduced form of ascorbate), MDA (malondialdehyde) and antioxidant enzymes suggest that magneto-priming alleviates oxidative stress in SMF-primed soybean plants. Field strength of 200 mT (1 h) proved to be more effective in improving all the parameters as compared to 150 mT. Our study suggested that pre-sowing SMF treatment can be efficaciously employed for improving the growth, development and production of soybean.
大豆(Glycine max)是世界上最重要的蛋白质和油料作物之一,需求量很大。为了满足这一需求,人们采用了各种现代农业方法,其中磁激发(用磁场处理种子)因其高效和环保的性质而成为最受欢迎的技术,以提高大豆植物的种子活力,生长和产量。为此,本研究通过田间试验,研究了静磁场诱导对大豆品种JS-335种子的影响。我们使用150 mT (1 h)和200 mT (1 h)的静磁场(SMF)强度进行研究。SMF处理均提高了植株的生长(茎部和根系生长参数)、固碳(PSII效率、气体交换参数、碳酸酐酶活性)和固氮(豆红蛋白含量、总蛋白含量、硝酸还原酶活性)。我们观察到这些参数之间存在关联,这些参数有助于生物量积累,从而提高作物产量。此外,ASA(还原型抗坏血酸)、MDA(丙二醛)和抗氧化酶水平的降低表明,磁启动减轻了smf启动大豆植株的氧化应激。结果表明,与150 mT相比,200 mT (1 h)的田间强度能更有效地改善所有参数。本研究表明,播前SMF处理可有效地改善大豆的生长发育和产量。
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引用次数: 2
Evaluation of Germination under Different Storage Conditions of Four Endemic Plant Species from Ethiopia: Implications for Ex Situ Conservation in Seed Banks 埃塞俄比亚四种特有植物在不同贮藏条件下萌发的评价:对种子库迁地保护的启示
Pub Date : 2023-02-06 DOI: 10.3390/seeds2010005
Sinework Dagnachew, D. Teketay, S. Demissew, Tesfaye Awas
The conservation of endemic plant species is a major concern, as the species are with restricted distribution range. Since in situ conservation alone will not guarantee their maintenance, ex situ conservation measures must be undertaken to support the conservation of these species. Investigation of the impact of the storage environment of seeds gives baseline information. Therefore, this study was conducted to investigate the effect of different storage conditions (room temperature, 4 °C and −10 °C) and different storage periods over one year. Four Ethiopian endemic plant species, namely Euryops pinifolius, Kniphofia foliosa, Lobelia rhynchopetalum, and Solanecio gigas, were considered. Multivariate analyses revealed a significant (p < 0.05) effect of storage condition and period on seed germination. The storage of seeds at room temperature before drying may not be recommended for short-term storage of the studied species, except K. foliosa. After drying of seeds, E. pinifolius, K. foliosa, and L. rhynchopetalum seeds showed high germination percentage (above 80%) after storage even at sub-zero temperatures for one year. The germination percentage of S. gigas stored at room temperature before drying for thirteen months was 60% and that stored at 4 °C was 6%, which indicates that the seeds may be categorized under intermediate storage behavior. The three species can be grouped in the orthodox seed storage category. Research on desiccation screening should be undertaken to predict an optimal combination of storage moisture content and suitable storage temperature to determine storage category with certainty.
由于特有植物的分布范围有限,其保护是一个值得关注的问题。由于单靠就地保护并不能保证它们的维持,因此必须采取移地保护措施来支持对这些物种的保护。对种子储存环境影响的调查提供了基线信息。因此,本研究对不同的储存条件(室温、4°C和- 10°C)和一年以上的不同储存期的影响进行了研究。研究了埃塞俄比亚特有植物Euryops pinifolius、Kniphofia foliosa、Lobelia rhynchopetalum和Solanecio gigas。多因素分析显示,贮藏条件和贮藏时间对种子萌发有显著影响(p < 0.05)。在干燥前在室温下储存种子可能不建议短期储存所研究的物种,除了K. foliosa。经干燥处理的松叶栎、叶叶栎和小叶栎种子在零下1年的低温下,发芽率均在80%以上。干燥前室温贮藏13个月的种子发芽率为60%,4℃贮藏13个月的种子发芽率为6%,表明种子可能属于中间贮藏行为。这3种植物可归为正统种子贮藏类。应进行干燥筛选研究,预测贮藏水分和适宜贮藏温度的最佳组合,从而确定贮藏种类。
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引用次数: 0
Acknowledgment to the Reviewers of Seeds in 2022 感谢2022年《种子》审稿人
Pub Date : 2023-01-19 DOI: 10.3390/seeds2010004
High-quality academic publishing is built on rigorous peer review [...]
高质量的学术出版建立在严格的同行评审的基础上[…]
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引用次数: 0
Prevalence and Management of Phytopathogenic Seed-Borne Fungi of Maize 玉米植物致病性种传真菌的流行与防治
Pub Date : 2023-01-09 DOI: 10.3390/seeds2010003
Rehema Erasto, N. Kilasi, R. Madege
Seed-borne fungi are solemn and deleterious pathogens capable of causing significant losses of quantity and quality losses in maize seeds and seedlings. They infect the crop at all points of the production chain from farms to stores. A yield loss of up to 50% can be encountered. Currently, chemical control of the disease is being implemented, though it is accompanied by several negative effects. This study aimed at identifying seed-borne fungi of maize and effective management options. A deep-freezing blotter method and morphological identification of the fungal species were implemented. The seed-borne fungi detected were Fusarium verticillioides, Aspergillus flavus, Aspergillus niger, Penicillium spp., Rhizopus spp., and Curvularia spp. However, in farmer-saved seeds, fungal incidences were significantly higher (p < 0.01) than in certified seeds. To identify more effective management options, the efficacy of water and ethanol-extracted bio-fungicides from three plant species, namely, neem (Azadirachta indica), ginger (Zingiber officinale), and coffee (Coffea arabica) were evaluated. From in vitro assays, ethanol-extracted bio-fungicides have a 100% inhibitory effect on fungal growth, whilst the inhibitory effects of water-extracted bio-fungicides are 55.88% (Azadirachta indica) and 46.31% (Zingiber officinale), followed by 5.15% (Coffea arabica). For the case of an in vivo assay, maize seeds treated with water-extracted bio-fungicides have higher seed germination and seedling vigor percentages. For germination, seeds treated with water-extracted bio-fungicides have higher percentages (neem and ginger (90%) followed by coffee (72.5%)) than ethanol-extracted bio-fungicides (neem (0%), ginger (2.5%), and coffee (0%)). A similar observation is made for seedling weight. Therefore, the tested water-extracted bio-fungicides can be used in treating seeds before sowing them. Further studies on effective methods of extracting bioactive compounds, and improving their shelf life, are recommended.
种传真菌是一种严重的有害病原体,能够造成玉米种子和幼苗数量和质量的重大损失。它们在从农场到商店的生产链的各个环节感染作物。产量损失可能高达50%。目前,正在实施对该疾病的化学控制,尽管它伴随着一些负面影响。本研究旨在鉴定玉米的种传真菌和有效的管理方案。采用深冻吸墨纸法对真菌种类进行了形态学鉴定。检出的种传真菌有黄曲霉、黄曲霉、黑曲霉、青霉、根霉、曲霉等,但农民自备种子的真菌感染率显著高于认证种子(p < 0.01)。为了确定更有效的管理方案,从三种植物即印楝(Azadirachta indica)、生姜(Zingiber officinale)和咖啡(Coffea arabica)中提取水和乙醇提取生物杀菌剂的效果进行了评估。体外实验结果表明,乙醇提取的生物杀菌剂对真菌生长的抑制效果为100%,水提取的生物杀菌剂对印楝的抑制效果分别为55.88%、46.31%、5.15%。在体内试验的情况下,用水提生物杀菌剂处理的玉米种子有更高的种子发芽率和幼苗活力百分比。对于种子的发芽率,水提取生物杀菌剂处理的种子的发芽率(印楝和生姜(90%),其次是咖啡(72.5%))高于乙醇提取生物杀菌剂处理的种子(印楝(0%),生姜(2.5%)和咖啡(0%))。对幼苗的重量也作了类似的观察。因此,所试验的水提生物杀菌剂可用于种子播前处理。建议进一步研究提取生物活性化合物的有效方法,并延长其保质期。
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引用次数: 1
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Seeds (New York, N.Y.)
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