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Impact of chronological ageing on semen parameters in southern Indian men visiting infertility centre: A retrospective study 时间老化对访问不育中心的印度南部男性精液参数的影响:一项回顾性研究
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.365227
M. Asif, A. Vijay, Maheshwari, Syed Fyzullah, Usha Rani, R. Swathi, K. D. Damodara Gowda
Objective: To investigate the association between age and semen parameters among male partners of subfertile couples. Methods: This retrospective study analyzed the semen of 1 523 infertile men aged 26 to 50 years. Data were extracted from GarbhaGudi IVF Centre database from January 2019 to September 2020. The basic semen parameters were interpreted according to the WHO manual 2021, 6th edition. Semen parameters in different age groups were compared. Results: Total and progressive motile sperms were significantly higher in the age group of 26-30 years compared to other age groups (P<0.05). Normal sperm count was significantly higher in the age group of 26-30 years compared to the age groups of 41-45 years and >46 years (P=0.001). However, sperm head defects, neck and midpiece defects, tail defects, and cytoplasmic droplets showed statistically insignificant difference in all the age groups (P>0.05). Semen viscosity showed no statistical difference in all the age groups compared to the reference age group of 26 to 30 years. Conclusions: Higher age can lead to a significant decrease in normal sperms and motility in subfertile men. Hence, male partner age should be considered as one of the major determining factors for reproductive outcomes.
目的:探讨不孕不育夫妇男性伴侣精液参数与年龄的关系。方法:回顾性分析1 523例26 ~ 50岁不育男性的精液。数据取自GarbhaGudi IVF中心2019年1月至2020年9月的数据库。精液基本参数根据世界卫生组织手册2021年第6版进行解释。比较不同年龄组的精液参数。结果:26-30岁年龄组总精子数和进行性活动精子数明显高于其他年龄组(P46岁,P=0.001)。而精子头部缺陷、颈中段缺陷、尾部缺陷、细胞质液滴在各年龄组间差异均无统计学意义(P < 0.05)。精液黏度各年龄组与参考年龄组26 ~ 30岁比较无统计学差异。结论:年龄增大可导致不育男性正常精子和活力显著下降。因此,男性伴侣年龄应被视为生殖结果的主要决定因素之一。
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引用次数: 0
Effects of N-acetylcysteine on growth, viability and reactive oxygen species levels in small antral follicles cultured in vitro n -乙酰半胱氨酸对体外培养小腔卵泡生长、活力和活性氧水平的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.365231
Efigênia Cordeiro, B. Silva, L. Paulino, P. Barroso, L. Barrozo, Miguel F De Lima Neto, J. Silva
Objective: To investigate the effects of different concentrations of N-acetylcysteine on follicular growth and morphology, as well as on viability, levels of reactive oxygen species (ROS) and meiotic progression of oocytes from in vitro cultured bovine early antral follicles. Methods: Isolated early antral follicles (about 500 μm) were cultured in TCM-199+ alone or supplemented with 1.0, 5.0 or 25.0 mM N-acetylcysteine at 38.5 °C with 5% CO2 for 8 days. Follicle diameters were evaluated at day 0, 4 and 8 of culture. At the end of culture, the levels of ROS, chromatin configuration and viability (calcein-AM and ethidium homodimer-1 staining) were investigated in the cumulus-oocyte complexes. Comparisons of follicle diameters between treatments were performed. Data on percentages of morphologically normal follicles, growth rates and chromatin configuration in different treatments were compared. Results: An increase in follicular diameters after culture in all treatments was observed, except for follicles cultured with 25.0 mM N-acetylcysteine. Fluorescence microscopy showed that oocytes cultured in all treatments were stained positively with calcein-AM, and that 5.0 mM N-acetylcysteine reduced fluorescence for ethidium homodimer-1. Intracellular levels of ROS in oocytes from follicles cultured with 1.0 mM N-acetylcysteine showed a significant reduction compared to other treatments. The presence of N-acetylcysteine in culture medium did not influence the rates of oocyte at the germinal vesicle stage. Conclusions: N-acetylcysteine at concentrations of 1.0 and 5.0 mM reduces ROS levels and staining for ethidium homodimer-1 in in vitro cultured follicles, respectively, while 25.0 mM N-acetylcysteine decreases follicular growth and the percentages of continuously growing follicles.
目的:研究不同浓度的N-乙酰半胱氨酸对体外培养的牛早期窦卵泡卵母细胞的生长和形态、活力、活性氧水平和减数分裂进程的影响。方法:分离的早期窦状卵泡(约500μm)在单独或补充1.0、5.0或25.0mM N-乙酰半胱氨酸的TCM-199+中,在38.5°C和5%CO2下培养8天。在培养的第0、4和8天评估卵泡直径。培养结束时,研究卵丘-卵母细胞复合物中ROS水平、染色质结构和活力(钙黄绿素AM和乙锭同源二聚体-1染色)。对不同治疗之间的卵泡直径进行比较。比较了不同处理中形态正常卵泡的百分比、生长率和染色质结构的数据。结果:除用25.0mM N-乙酰半胱氨酸培养的卵泡外,在所有处理中均观察到培养后卵泡直径增加。荧光显微镜显示,在所有处理中培养的卵母细胞均被钙黄绿素AM阳性染色,并且5.0mM N-乙酰半胱氨酸降低了同二聚乙锭-1的荧光。与其他处理相比,用1.0mM N-乙酰半胱氨酸培养的卵泡卵母细胞中ROS的细胞内水平显著降低。培养基中N-乙酰半胱氨酸的存在不影响卵母细胞在生发泡阶段的比率。结论:浓度为1.0和5.0 mM的N-乙酰半胱氨酸分别降低体外培养卵泡中的ROS水平和同二聚乙锭-1的染色,而25.0 mM的N乙酰半胱氨酸降低卵泡生长和持续生长卵泡的百分比。
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引用次数: 0
A novel herbal combination ameliorates ovarian dysfunction and regulates altered biochemical parameters in rats with letrozole-induced polycystic ovary syndrome 一种新的草药组合改善来曲唑诱导的多囊卵巢综合征大鼠的卵巢功能障碍并调节其改变的生化参数
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.365229
S. Shailajan, S. Menon, Swati Singh, Yugandhara M Patil
Objective: To investigate the potential activity of novel herbal combination and novel herbal formulation (syrup) in female Sprague Dawley rats with letrozole-induced polycystic ovary syndrome (PCOS). Methods: Forty-two rats were randomly divided into seven groups with six rats in each group. Group 1 received 0.5% carboxy methylcellulose orally for 37 days and served as the normal control group. Group 2 was orally administered with letrozole of 1 mg/kg for 21 days and served as the PCOS induction group without treatment. Group 3 to 7 were administered with letrozole of 1 mg/kg for 21 days orally to induce PCOS, and then respectively received clomiphene citrate at 1 mg/kg, 100 and 200 mg/kg of novel herbal combination, 200 mg/kg of novel herbal formulation (syrup), and 400 mg/kg of marketed formulation of Pushyanuga churna, once daily for 15 days. Effects of the novel herbal combination and its syrup formulation were evaluated on the hormonal profile, the levels of antioxidants, the lipid profile and on the ovarian morphology, using letrozole-induced PCOS model in rats. Results: Letrozole caused alterations in hormonal levels and lipid levels similar to PCOS and ovarian histology showed presence of ovarian cysts confirming the induction of PCOS in rats. On treatment with the novel herbal combination and its syrup formulation in PCOS-induced rats, the altered hormonal and lipid profiles showed significant recovery to normal levels. Ovarian histology confirmed the restoration of folliculogenesis in the PCOS-induced rats. The treatment with the syrup formulation of novel herbal combination was found to be more effective than novel herbal combination and showed better recovery in various parameters evaluated. The results of the study, however, suggested that treatment with novel herbal combination and its syrup formulation provided minimal protection against oxidative stress caused due to the induction of PCOS. Conclusions: The integrated approach for management of PCOS is to counterbalance the limitations associated with modern therapy. Both the novel herbal combination and the syrup formulation of novel herbal combination show efficacy in the management of PCOS in rats and restore folliculogenesis in the ovary. The syrup formulation of novel herbal combination is most effective in the management of PCOS and shows potential to be developed as an adjuvant therapeutic agent.
目的:研究新草药组合和新草药制剂(糖浆)对来曲唑诱导的多囊卵巢综合征(PCOS)雌性Sprague-Dawley大鼠的潜在活性。方法:将42只大鼠随机分为7组,每组6只。第1组口服0.5%羧甲基纤维素37天,作为正常对照组。第2组口服来曲唑1mg/kg,持续21天,作为不治疗的PCOS诱导组。第3至第7组口服1 mg/kg的来曲唑21天以诱导PCOS,然后分别接受1 mg/kg、100和200 mg/kg的新型草药组合、200 mg/kg的新草药制剂(糖浆)和400 mg/kg的Pushyanuga churna市售制剂的克罗米芬柠檬酸盐,每天1次,持续15天。使用来曲唑诱导的大鼠多囊卵巢综合征模型,评估了新草药组合及其糖浆制剂对激素水平、抗氧化剂水平、脂质水平和卵巢形态的影响。结果:来曲唑引起的激素水平和脂质水平的变化与多囊卵巢综合征相似,卵巢组织学显示存在卵巢囊肿,证实了大鼠多囊卵巢综合症的诱导。在PCOS诱导的大鼠中,用新的草药组合及其糖浆制剂治疗时,激素和脂质的变化显示出显著恢复到正常水平。卵巢组织学证实多囊卵巢综合征诱导的大鼠卵泡发生恢复。新草药组合的糖浆制剂的治疗被发现比新草药组合更有效,并且在评估的各种参数中显示出更好的回收率。然而,研究结果表明,使用新型草药组合及其糖浆制剂进行治疗,对PCOS诱导引起的氧化应激的保护作用微乎其微。结论:多囊卵巢综合征的综合治疗方法是平衡现代治疗的局限性。新草药组合和新草药组合的糖浆制剂均显示出对大鼠多囊卵巢综合征的治疗和恢复卵巢卵泡生成的疗效。新型草药组合的糖浆制剂在多囊卵巢综合征的治疗中最有效,并显示出作为辅助治疗剂开发的潜力。
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引用次数: 0
The fate of surplus embryos in the setting of assisted reproductive technology: A scoping review 辅助生殖技术环境下剩余胚胎的命运:范围界定综述
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.365226
I. Agung Sentosa, S. Ayuandari, Rafhani Rosyidah, Agung Dewanto
Objective: To identify the attitudes of infertile couples toward their surplus frozen embryos. Methods: This study was according to PRISMA-ScR as the guideline for scoping review. Studies that assessed the attitudes of patients or infertile couples who had surplus embryos were included. We conducted systematic searches in English studies from April 2011-April 2021 using 7 databases: PubMed, Science Direct, EBSCO, Scopus, the Cochrane Library, Sage Journals, and Google Scholar. Data were charted based on author, year of publication, country, purpose, data collection, key findings, and research focus/domain. Results: A total of 37 research articles were included in the analysis. Their attitudes encompassed: supporting the donation of the surplus embryos for both research and reproductive purposes, continuing to store the frozen embryos, and disposing of the surplus embryos. Conclusions: Most of the infertile patients support donating their surplus embryos for research and reproductive purposes.
目的:了解不孕夫妇对剩余冷冻胚胎的态度。方法:本研究以PRISMA ScR为指导进行范围审查。研究评估了胚胎过剩的患者或不孕夫妇的态度。从2011年4月到2021年4月,我们使用7个数据库对英语研究进行了系统搜索:PubMed、Science Direct、EBSCO、Scopus、Cochrane Library、Sage Journals和Google Scholar。数据是根据作者、出版年份、国家、目的、数据收集、关键发现和研究重点/领域绘制的。结果:共有37篇研究文章被纳入分析。他们的态度包括:支持捐赠多余的胚胎用于研究和生殖目的,继续储存冷冻胚胎,以及处理多余的胚胎。结论:大多数不孕患者支持将剩余胚胎捐献用于研究和生殖目的。
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引用次数: 0
Serum anti-leukemia inhibitory factor antibody and recurrent pregnancy loss in Iranian women 伊朗妇女血清抗白血病抑制因子抗体与复发性流产
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.365232
S. Seifati, Hossein Ansariniya, Fateme Rahmani, A. Saboor-Yaraghi, F. Fesahat, H. Hadinedoushan, F. Zare
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引用次数: 0
Bacteriospermia among smallholder artificial insemination boars in the Philippines and potential associated factors 菲律宾小型人工授精公猪细菌精子症及其潜在相关因素
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.365230
Santiago T. Peña Jr., M. Pagente, Bianca Ymas, Mark Janier
Objective: To determine the prevalence of bacteriospermia, the bacterial load, and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operators. Methods: Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte, Philippines and were subjected to standard bacteriological culture and identification, including a survey of potentially associated factors. Prevalence and bacterial count were determined accordingly, while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering technique. Results: One hundred percent contamination with a bacterial count of (2.01±0.38)×103 CFU/mL was observed. At least 73.33% of the samples were positive for Bacillus spp., while other identified isolates included Enterobacter spp., Staphylococcus spp., E. coli, Pseudomonas spp., Citrobacter spp., and Klebsiella spp. Conclusions: Despite the high prevalence of bacteriospermia, the bacterial count is low. Nevertheless, on-farm practices on boar health and management, semen collection, and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation.
目的:了解当地小农人工授精操作人员采集的猪精液中细菌精子症的流行情况、细菌载量及可能的细菌污染因素。方法:从菲律宾Leyte第5区不同的小农猪运营商拥有的当地可获得的人工授精公猪中收集了15个单独的生精液样本,并进行了标准的细菌培养和鉴定,包括对潜在相关因素的调查。据此确定流行率和细菌数量,同时采用聚类分层聚类技术对公猪特征和收集做法进行聚类。结果:污染100%,细菌计数为(2.01±0.38)×103 CFU/mL。至少73.33%的样本检出芽孢杆菌阳性,其他检出的菌株包括肠杆菌、葡萄球菌、大肠杆菌、假单胞菌、柠檬酸杆菌和克雷伯氏菌。结论:尽管菌精率较高,但细菌数量较低。然而,在小农人工授精操作中,应认真考虑猪场卫生和管理、精液收集和卫生以及加强控制污染的基本规程方面的做法。
{"title":"Bacteriospermia among smallholder artificial insemination boars in the Philippines and potential associated factors","authors":"Santiago T. Peña Jr., M. Pagente, Bianca Ymas, Mark Janier","doi":"10.4103/2305-0500.365230","DOIUrl":"https://doi.org/10.4103/2305-0500.365230","url":null,"abstract":"Objective: To determine the prevalence of bacteriospermia, the bacterial load, and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operators. Methods: Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte, Philippines and were subjected to standard bacteriological culture and identification, including a survey of potentially associated factors. Prevalence and bacterial count were determined accordingly, while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering technique. Results: One hundred percent contamination with a bacterial count of (2.01±0.38)×103 CFU/mL was observed. At least 73.33% of the samples were positive for Bacillus spp., while other identified isolates included Enterobacter spp., Staphylococcus spp., E. coli, Pseudomonas spp., Citrobacter spp., and Klebsiella spp. Conclusions: Despite the high prevalence of bacteriospermia, the bacterial count is low. Nevertheless, on-farm practices on boar health and management, semen collection, and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"35 - 41"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48159872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reproductive outcomes of water pipe smoking: A scoping review 水烟吸烟的生殖结果:范围综述
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.386122
Fardin Amidi, Sahar Rostami, Farzane Fereidouni, Arezoo Maleki-Hajiagha, Mohadese Motaharinejad, Somayye Majidi
Objective: To present an overview of the adverse effects of water pipe smoking on various aspects of reproduction and perinatal complications, covering all the most relevant studies, from descriptive findings to clinical trials. Methods: We reviewed the literature and included full publications in English provided by international biomedical databases, including PubMed, Science Direct, EBSCO, Scopus, and Google Scholar, and Cochrane Library over the last 38 years (May 1985 to June 2023). Search terms (Water pipe, Narghile, Shisha, Dokha, etc., and Reproduction, Fertility, etc.) were adopted from Medical Subheading (MeSH) and Boolean operators were utilized to improve sensitivity. Results: 133 Papers were identified in the initial search, of which 29 were excluded due to duplication. Eliminating search overlap and irrelevant records by reviewing titles and abstracts reduced 42 papers. 62 Full-publication articles met the inclusion criteria. We summarized the effects of waterpipe smoke exposure on reproductive health based on three main categories: male and female fertility, fetal and neonatal outcomes, and pregnancy complications. Conclusions: Water pipe smoking has potential adverse effects on different aspects of human reproductive health, including semen parameters, male and female fertility, and fetal and neonatal health, and contributes to many maternal complications and morbidities.
目的:概述水烟对生殖和围产期并发症各方面的不良影响,涵盖所有最相关的研究,从描述性发现到临床试验。方法:我们回顾了近38年来(1985年5月至2023年6月)国际生物医学数据库PubMed、Science Direct、EBSCO、Scopus、Google Scholar和Cochrane Library提供的文献,并纳入了全文英文出版物。医学副标题(MeSH)采用了水管、Narghile、水烟、Dokha等检索词和生殖、生育等检索词,并利用布尔运算符提高灵敏度。结果:在初始检索中发现133篇论文,其中29篇因重复被排除。通过审查题目和摘要来消除搜索重叠和不相关的记录,减少了42篇论文。62篇完整发表的文章符合纳入标准。我们从男性和女性生育能力、胎儿和新生儿结局以及妊娠并发症这三个主要方面总结了水烟暴露对生殖健康的影响。结论:水烟吸烟对人类生殖健康的不同方面有潜在的不良影响,包括精液参数、男性和女性生育能力、胎儿和新生儿健康,并导致许多产妇并发症和发病率。
{"title":"Reproductive outcomes of water pipe smoking: A scoping review","authors":"Fardin Amidi, Sahar Rostami, Farzane Fereidouni, Arezoo Maleki-Hajiagha, Mohadese Motaharinejad, Somayye Majidi","doi":"10.4103/2305-0500.386122","DOIUrl":"https://doi.org/10.4103/2305-0500.386122","url":null,"abstract":"Objective: To present an overview of the adverse effects of water pipe smoking on various aspects of reproduction and perinatal complications, covering all the most relevant studies, from descriptive findings to clinical trials. Methods: We reviewed the literature and included full publications in English provided by international biomedical databases, including PubMed, Science Direct, EBSCO, Scopus, and Google Scholar, and Cochrane Library over the last 38 years (May 1985 to June 2023). Search terms (Water pipe, Narghile, Shisha, Dokha, etc., and Reproduction, Fertility, etc.) were adopted from Medical Subheading (MeSH) and Boolean operators were utilized to improve sensitivity. Results: 133 Papers were identified in the initial search, of which 29 were excluded due to duplication. Eliminating search overlap and irrelevant records by reviewing titles and abstracts reduced 42 papers. 62 Full-publication articles met the inclusion criteria. We summarized the effects of waterpipe smoke exposure on reproductive health based on three main categories: male and female fertility, fetal and neonatal outcomes, and pregnancy complications. Conclusions: Water pipe smoking has potential adverse effects on different aspects of human reproductive health, including semen parameters, male and female fertility, and fetal and neonatal health, and contributes to many maternal complications and morbidities.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135009130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of small non-coding RNAs (sncRNAs) in male infertility: A scoping review 小非编码rna (sncRNAs)在男性不育中的作用:范围综述
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.386121
Hacer Kaya Cakir, Onur Eroglu
Objective: To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA (sncRNA) may hold in relation to improving the treatment and diagnosis of male infertility. Methods: PRISMA-ScR was used as the scoping review guideline for this investigation. All article data here have been accessed from MEDLINE–PubMed, Science Direct, EBSCO, Scopus, Sage Journals, and Google Scholar. The terms "small non coding RNA, male, infertility, miRNA, sperm" were used in the search between 2015 and 2023. Results: The study comprised 35 publications in total. Several sncRNAs, miR-155, miR-16, miR-196, miR-525-3p, miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men. sncRNA can be used as a biomarker of male infertility. Conclusions: sncRNAs can act as biomarkers for the diagnosis of reproductive diseases. Actually, by recognizing sncRNAs and their mechanisms, a new way to treat infertile men would be paved. The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential. This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.
目的:简要介绍表观遗传学领域的研究进展,以及小分子非编码RNA (sncRNA)在改善男性不育症治疗和诊断方面的潜在预测能力。方法:采用PRISMA-ScR作为本研究的范围审查指南。本文的所有文章数据均来自MEDLINE-PubMed、Science Direct、EBSCO、Scopus、Sage Journals和Google Scholar。2015年至2023年期间的搜索使用了“小非编码RNA,男性,不育,miRNA,精子”等术语。结果:本研究共纳入35篇文献。研究发现,miR-155、miR-16、miR-196、miR-525-3p、miR-891等几种sncrna在男性不育症中有效调节精子加工机制。sncRNA可作为男性不育的生物标志物。结论:sncRNAs可作为生殖疾病诊断的生物标志物。事实上,通过识别sncrna及其机制,一种治疗不育症的新方法将铺平道路。sncRNAs在精子发生中的功能注释仍处于起步阶段,但具有巨大的潜力。尽管使用尖端技术和可公开访问的sncRNA注释工具,迄今为止已经发现了许多潜在的sncRNA。
{"title":"The role of small non-coding RNAs (sncRNAs) in male infertility: A scoping review","authors":"Hacer Kaya Cakir, Onur Eroglu","doi":"10.4103/2305-0500.386121","DOIUrl":"https://doi.org/10.4103/2305-0500.386121","url":null,"abstract":"Objective: To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA (sncRNA) may hold in relation to improving the treatment and diagnosis of male infertility. Methods: PRISMA-ScR was used as the scoping review guideline for this investigation. All article data here have been accessed from MEDLINE–PubMed, Science Direct, EBSCO, Scopus, Sage Journals, and Google Scholar. The terms \"small non coding RNA, male, infertility, miRNA, sperm\" were used in the search between 2015 and 2023. Results: The study comprised 35 publications in total. Several sncRNAs, miR-155, miR-16, miR-196, miR-525-3p, miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men. sncRNA can be used as a biomarker of male infertility. Conclusions: sncRNAs can act as biomarkers for the diagnosis of reproductive diseases. Actually, by recognizing sncRNAs and their mechanisms, a new way to treat infertile men would be paved. The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential. This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135010277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of taxifolin on cold-shock damages in spermatozoa in rabbits 紫杉醇对兔精子冷休克损伤的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.386125
İbrahimHalil Güngör, İrfan Yılmaz, Gaffari Türk, AslıhanÇakır Cihangiroğlu, TutkuCan Acısu, Gözde Arkalı, EdanurGüler Ekmen
Objective: To investigate the effect of taxifolin added to rabbit semen on freezing-induced cold-shock damages in spermatozoa. Methods: Semen was collected from six adult New Zealand rabbits once a week by artificial vagina. The collected semen was pooled at 38 °C and divided into four equal volumes. They were diluted with 0, 50, 100 and 200 μM taxifolin-containing Tris + egg yolk extender at 38 °C and their temperatures were lowered to 4 °C. Following equilibration, semen drawn into 0.25 mL straws were frozen in an automatic semen freezing device and stored in liquid nitrogen container at -196 °C. Samples were thawed in 38 °C water for 25 s and the analyses of motility, kinematic parameters, morphological deformities, changes in membrane integrity, mitochondrial membrane potential, dead-live ratio, acrosomal damages and as well as oxidative stress analyses were performed in semen. Results: Addition of 50 μM taxifolin significantly improved motility (total, progressive, rapid and static), high mitochondrial membrane potential and the ratios of spermatozoa with acrosomal damage compared to the control group. Compared to the control group, malondialdehyde (MDA) levels in the 50 and 100 μM taxifolin groups were significantly lower, while the MDA level was high and viable spermatozoa ratio was low in the 200 μM taxifolin group. There were no significant differences between the groups in terms of kinematic parameters, morphological deformities, membrane integrity and antioxidant levels. Conclusions: The low dose of taxifolin (50 μM) has a positive effect and the high dose (200 μM) has a negative effect. Therefore, it is concluded that the addition of low-dose (50 μM) taxifolin to the extenders would be a useful additive in reducing cold-shock damage that occurs during freezing of rabbit semen.
目的:探讨兔精液中添加杉藻素对冷冻致精子冷休克损伤的影响。方法:采用人工阴道采集成年新西兰兔6只,每周1次。收集的精液在38°C下存放,分成四等份。分别用0、50、100、200 μM含taxifolin的Tris +蛋黄扩展剂在38℃下稀释,温度降至4℃。平衡后,抽取0.25 mL吸管的精液在精液自动冷冻装置中冷冻,并在-196°C的液氮容器中保存。样品在38°C水中解冻25 s,分析精液的运动性、运动学参数、形态畸形、膜完整性变化、线粒体膜电位、死活比、顶体损伤和氧化应激分析。结果:与对照组相比,添加50 μM紫杉醇可显著改善精子的运动能力(总、渐进、快速和静态),提高线粒体膜电位和顶体损伤的精子比例。与对照组相比,50和100 μM taxifolin组丙二醛(MDA)水平显著降低,而200 μM taxifolin组丙二醛(MDA)水平较高且活精子率较低。各组之间在运动参数、形态变形、膜完整性和抗氧化水平方面无显著差异。结论:低剂量(50 μM)杉木素有积极作用,高剂量(200 μM)有消极作用。因此,在扩增剂中添加低剂量(50 μM)的杉木素可有效降低兔精液冷冻过程中的冷休克损伤。
{"title":"Effect of taxifolin on cold-shock damages in spermatozoa in rabbits","authors":"İbrahimHalil Güngör, İrfan Yılmaz, Gaffari Türk, AslıhanÇakır Cihangiroğlu, TutkuCan Acısu, Gözde Arkalı, EdanurGüler Ekmen","doi":"10.4103/2305-0500.386125","DOIUrl":"https://doi.org/10.4103/2305-0500.386125","url":null,"abstract":"Objective: To investigate the effect of taxifolin added to rabbit semen on freezing-induced cold-shock damages in spermatozoa. Methods: Semen was collected from six adult New Zealand rabbits once a week by artificial vagina. The collected semen was pooled at 38 °C and divided into four equal volumes. They were diluted with 0, 50, 100 and 200 μM taxifolin-containing Tris + egg yolk extender at 38 °C and their temperatures were lowered to 4 °C. Following equilibration, semen drawn into 0.25 mL straws were frozen in an automatic semen freezing device and stored in liquid nitrogen container at -196 °C. Samples were thawed in 38 °C water for 25 s and the analyses of motility, kinematic parameters, morphological deformities, changes in membrane integrity, mitochondrial membrane potential, dead-live ratio, acrosomal damages and as well as oxidative stress analyses were performed in semen. Results: Addition of 50 μM taxifolin significantly improved motility (total, progressive, rapid and static), high mitochondrial membrane potential and the ratios of spermatozoa with acrosomal damage compared to the control group. Compared to the control group, malondialdehyde (MDA) levels in the 50 and 100 μM taxifolin groups were significantly lower, while the MDA level was high and viable spermatozoa ratio was low in the 200 μM taxifolin group. There were no significant differences between the groups in terms of kinematic parameters, morphological deformities, membrane integrity and antioxidant levels. Conclusions: The low dose of taxifolin (50 μM) has a positive effect and the high dose (200 μM) has a negative effect. Therefore, it is concluded that the addition of low-dose (50 μM) taxifolin to the extenders would be a useful additive in reducing cold-shock damage that occurs during freezing of rabbit semen.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135008590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methanolic pomegranate dried peel extract improves cryopreserved semen quality and antioxidant capacity of rams 甲醇石榴干皮提取物可提高公羊冻存精液质量和抗氧化能力
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-01-01 DOI: 10.4103/2305-0500.386124
AmalM Aboelmaaty, MohamedS Kotp, AyaM Fadl, ElshymaaA Abdelnaby, IslamE El-Seadawy, HossamR El-Sherbiny
Objective: To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams' semen extender for obtaining the best-cryopreserved semen quality. Methods: Tris-based semen extender was supplemented with 0.0, 0.40, 0.48, and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months (n=80). Pooled (n=16) post-thaw semen characteristics were determined. Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GPx), superoxide dismutase (SOD), ascorbic acid, zinc, copper, total cholesterol, low-density lipoproteins (LDL), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP). Results: The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility (P<0.001), sperm progressive motility (P<0.001), live sperm (P<0.001), sperm plasma membrane integrity (P<0.001), acrosome integrity (P<0.001), SOD (P<0.05), zinc (P<0.001), total cholesterol (P<0.001), and LDL (P<0.001) with the lowest percentage of abnormal sperm morphology (P<0.001), the lowest lipid peroxidation (MDA, P<0.01), ascorbic acid (P>0.05), and LDH (P>0.05). Conclusions: Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.
目的:选择适宜浓度的甲醇石榴提取物添加到公羊精液填充剂中,以获得最佳的冷冻保存精液质量。方法:以tris为基础的增精剂分别添加0.0、0.40、0.48和0.56 mg/mL石榴皮甲醇提取物,每周2次延长5只本地公羊的精液,连续2个月(n=80)。共(n=16)测定解冻后精液特征。各添加组和对照组解冻后的精血浆检测丙二醛(MDA)、一氧化氮(NO)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、抗坏血酸、锌、铜、总胆固醇、低密度脂蛋白(LDL)、乳酸脱氢酶(LDH)和碱性磷酸酶(ALP)。结果:以tris为基础的精液扩展剂添加0.48 mg/mL的精液扩展剂,解冻后精子总活力和LDH最高(P0.05)。结论:添加0.48 mg/mL石榴皮甲醇提取物于tris型公羊增精剂中,可通过提高生化指标和抗氧化能力来保存公羊解冻后的精子特征。
{"title":"Methanolic pomegranate dried peel extract improves cryopreserved semen quality and antioxidant capacity of rams","authors":"AmalM Aboelmaaty, MohamedS Kotp, AyaM Fadl, ElshymaaA Abdelnaby, IslamE El-Seadawy, HossamR El-Sherbiny","doi":"10.4103/2305-0500.386124","DOIUrl":"https://doi.org/10.4103/2305-0500.386124","url":null,"abstract":"Objective: To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams' semen extender for obtaining the best-cryopreserved semen quality. Methods: Tris-based semen extender was supplemented with 0.0, 0.40, 0.48, and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months (n=80). Pooled (n=16) post-thaw semen characteristics were determined. Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GPx), superoxide dismutase (SOD), ascorbic acid, zinc, copper, total cholesterol, low-density lipoproteins (LDL), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP). Results: The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility (P<0.001), sperm progressive motility (P<0.001), live sperm (P<0.001), sperm plasma membrane integrity (P<0.001), acrosome integrity (P<0.001), SOD (P<0.05), zinc (P<0.001), total cholesterol (P<0.001), and LDL (P<0.001) with the lowest percentage of abnormal sperm morphology (P<0.001), the lowest lipid peroxidation (MDA, P<0.01), ascorbic acid (P>0.05), and LDH (P>0.05). Conclusions: Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135009117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Asian Pacific Journal of Reproduction
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