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Expression of VEGF165 and VEGF165b during ovarian follicular development 卵巢卵泡发育过程中 VEGF165 和 VEGF165b 的表达
Q1 Veterinary Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390303
Chinnarat Changsangfa, Bongkoch Turathum, Morakot Sroyraya, Khwanthana Grataitong
To investigate the role of vascular endothelial growth factor (VEGF)165a, VEGF165b, and VEGF receptor (VEGFR) in the development of bovine follicles. We cultured follicular cells that were collected from small, medium, and large sized bovine follicles with estrogen and measured the expression of VEGF, VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence. The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium. The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h. VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h. In addition, VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h. This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner. This reveals an advantage during oocyte maturation in vitro.
研究血管内皮生长因子(VEGF)165a、VEGF165b 和血管内皮生长因子受体(VEGFR)在牛卵泡发育过程中的作用。 我们用雌激素培养了从小号、中号和大号牛卵泡中收集的卵泡细胞,并通过 Western 印迹分析和免疫荧光法测定了 VEGF、VEGFR2 和 VEGF165b 的表达。 在含有雌激素的培养基中培养后,所有大小的卵泡中 VEGF165 的表达量都有所增加,小卵泡和大卵泡中 VEGF165b 的表达量也有所增加。这些证据表明,在牛卵泡发育过程中,VEGF165 和 VEGFR 的表达与雌激素刺激有关,并且是以自分泌或旁分泌的方式表达的。这揭示了体外卵母细胞成熟过程中的优势。
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引用次数: 0
Oxidative stress in male infertility and therapeutic approach: A mini-review 男性不育症中的氧化应激与治疗方法:微型综述
Q1 Veterinary Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390299
Marwa Lahimer, H. Mustapha, Véronique Bach, H. Khorsi-Cauet, Moncef Benkhalifa, Mounir Ajina, Habib Ben Ali
Growing evidence suggests that oxidative stress is a major cause of male infertility. Spermatozoa are highly sensitive cells due to the vulnerability of their membrane fatty acids and the diminished antioxidant defense. Reactive oxygen species (ROS) impact multiple signaling pathways involved in mitochondrial dysfunction, hormonal unbalance and semen quality decline. The origin of ROS is diverse, including generated normal cellular metabolism, environmental exposure, advanced paternal age and inflammation. Research has indicated that antioxidant supplementation can improve oxidative stress and reduce the risk of chronic diseases. Moreover, it is widely known that antioxidant supplementation can enhance sperm quality and increase the pregnancy rate in couples undergoing fertility treatments. The current study highlights the deleterious effect of ROS and its impact on semen function. In addition, it contributes to the development of a clinical approach for the use of antioxidants in the management of male infertility.
越来越多的证据表明,氧化应激是导致男性不育的主要原因。由于精子膜脂肪酸的脆弱性和抗氧化防御能力的减弱,精子是高度敏感的细胞。活性氧(ROS)会影响多种信号通路,导致线粒体功能障碍、荷尔蒙失衡和精液质量下降。ROS 的来源多种多样,包括正常的细胞新陈代谢、环境暴露、高龄父亲和炎症。研究表明,补充抗氧化剂可以改善氧化应激,降低慢性疾病风险。此外,众所周知,补充抗氧化剂可以提高精子质量,增加接受生育治疗的夫妇的受孕率。本研究强调了 ROS 的有害作用及其对精液功能的影响。此外,这项研究还有助于开发一种临床方法,用于治疗男性不育症。
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引用次数: 0
Pregnancy outcomes following supplementation of single dose GnRH agonist to sustain the luteal phase in antagonist fresh embryo transfer cycles: A multicentric prospective cohort study 在拮抗剂新鲜胚胎移植周期中补充单剂量GnRH激动剂以维持黄体期后的妊娠结局:一项多中心前瞻性队列研究
Q1 Veterinary Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380983
Tatapudi Parvathi Devi, A. Gahlot, Sangita Sharma, M. Choudhary, R. Soni, Meeta Sharma
Objective: To determine whether a single dose of gonadotropin-releasing hormone (GnRH) agonist administered subcutaneously in addition to the regular progesterone supplementation could provide a better luteal support in antagonist protocol fresh embryo transfer cycles. Methods: This prospective, multicentric, cohort study included total 140 women, 70 in each group. Controlled ovarian stimulation was carried out as per fixed GnRH antagonist protocol. The trigger was given with hCG. In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) was performed and day-3 embryos were transferred. Patients were divided into groups 1 and 2 based on computer generated randomization sheet. Six days following oocyte retrieval, group 1 received 0.2 mg decapeptyl subcutaneously in addition to regular progesterone support while group 2 received progesterone only. Luteal support was given for 14 days to both groups; if pregnancy was confirmed luteal support was continued till 12 weeks of gestation. The clinical pregnancy rate was the primary outcome. The implantation rate, miscarriage rate, live birth delivery rate, and multiple pregnancy rates were the secondary outcomes. Results: A total of 140 patients were analysed, 70 in each group. Clinical pregnancy rates (47.1% vs. 35.7%; P=0.17), implantation rates (23.4% vs. 18.1%, P=0.24), live birth delivery rates (41.4% vs. 27.1%, P=0.08), and multiple pregnancy rates (21.2% vs. 16.0%, P=0.74) were higher in group 1 than in group 2. Group 1 had a lower miscarriage rate than group 2 (5.7% vs. 8.6%; P=0.75). However, these differences were not statistically significant between the two groups. Conclusions: Administration of a single dose of GnRH agonist in addition to regular natural micronized vaginal progesterone as luteal support in GnRH antagonist protocol cycles marginally improves implantation rates, clinical pregnancy rates, and live birth delivery rates. However, more studies with higher sample sizes are needed before any conclusive statements about GnRH agonist as luteal phase support can be made.
目的:探讨单剂量促性腺激素释放激素(GnRH)激动剂在常规黄体酮补充的基础上皮下注射是否能在拮抗剂方案的新鲜胚胎移植周期中提供更好的黄体支持。方法:这项前瞻性、多中心、队列研究共纳入140名女性,每组70名。对照卵巢刺激按照固定GnRH拮抗剂方案进行。触发剂是hCG。进行体外受精/胞浆内单精子注射(IVF/ICSI),移植第3天的胚胎。根据计算机生成的随机分组表将患者分为1组和2组。取卵6天后,组1在常规黄体酮支持的基础上皮下注射十肽0.2 mg,组2只注射黄体酮。两组均给予黄体支持14 d;如果确认怀孕,黄体支持持续到妊娠12周。临床妊娠率为主要观察指标。次要指标为着床率、流产率、活产率和多胎妊娠率。结果:共分析140例患者,每组70例。临床妊娠率(47.1% vs. 35.7%;P=0.17)、着床率(23.4% vs. 18.1%, P=0.24)、活产率(41.4% vs. 27.1%, P=0.08)、多胎妊娠率(21.2% vs. 16.0%, P=0.74)均高于组2。1组流产率低于2组(5.7%比8.6%;P = 0.75)。然而,这些差异在两组之间没有统计学意义。结论:在GnRH拮抗剂方案周期中,单剂量GnRH激动剂加常规天然微粉阴道孕酮作为黄体支持剂,可略微提高着床率、临床妊娠率和活产率。然而,在任何关于GnRH激动剂作为黄体期支持剂的结论性声明之前,需要更多样本量更大的研究。
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引用次数: 0
Plantain-based diet modulates atrazine-induced testicular toxicities in rats 以大车前草为基础的饮食调节阿特拉津诱导的大鼠睾丸毒性
Q1 Veterinary Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380984
D. Rotimi, O. Adeyemi
Objective: To assess the potential of plantain-based diet in modulating testicular toxicities in rats exposed to atrazine. Methods: The plantain-based diet at 50%, 25% and 12.5% were prepared from the basal diet by substituting the corn starch with plantain fruit pulp flour at different percentages. Wistar rats were fed plantain-based diet in varying concentrations ranging from 12.5% to 50% of the basal diet for 21 days before or after atrazine treatment in a two-phase experiment: preventive and therapeutic phases. The therapeutic model (n=35) had seven groups with 5 rats each, including the control, atrazine, atrazine recovery, atrazine plus plantain-based diet 50%, 25%, 12.5%, and atrazine plus quercetin groups. The preventive model (n=30) had six groups of 5 rats, consisting of the control, atrazine, 50%, 25%, 12.5% plantain-based diet plus atrazine, and quercetin plus atrazine groups. Gonadal hormones (testosterone, luteinizing hormone and follicle-stimulating hormone), sperm parameters (sperm motility, viability, morphology and concentration), and testicular function indices (protein, cholesterol, glycogen, acid phosphatase, alkaline phosphatase and lactate dehydrogenase) were measured. Results: The gonadal hormones, sperm characteristics, and testicular function indices of the rat testis decreased significantly in the atrazine group alongside degeneration of the histoarchitecture. However, plantain-based diet restored the gonadal hormone concentrations, semen parameters, and testicular function indices in both the preventive and therapeutic models. Conclusions: Treatment with plantain-based diet protects against rat testicular toxicity caused by atrazine via the modulation of gonadal hormones, sperm quality, testicular function index as well as histoarchitecture of rat testes.
目的:评价车前草为基础的饮食对阿特拉津暴露大鼠睾丸毒性的调节作用。方法:在基础饲粮的基础上,以不同比例的车前草果肉粉代替玉米淀粉,分别配制50%、25%和12.5%车前草基础饲粮。在阿特拉津治疗前后的21天内,Wistar大鼠被喂食以车前草为基础的饲料,其浓度在基础饲料的12.5%至50%之间,分为两阶段:预防和治疗阶段。治疗模型(n=35)分为7组,每组5只,包括对照组、阿特拉津组、阿特拉津恢复组、阿特拉津加车前草饲粮50%组、25%组、12.5%组和阿特拉津加槲皮素组。预防模型(n=30)设6组,每组5只,分为对照组、阿特拉津组、50%、25%、12.5%车前草饲粮加阿特拉津组和槲皮素加阿特拉津组。测定性腺激素(睾酮、黄体生成素和促卵泡激素)、精子参数(精子活力、活力、形态和浓度)和睾丸功能指标(蛋白质、胆固醇、糖原、酸性磷酸酶、碱性磷酸酶和乳酸脱氢酶)。结果:阿特拉津组大鼠睾丸性腺激素、精子特征和睾丸功能指标明显降低,组织结构变性。然而,以大车前草为基础的饮食在预防和治疗模型中均能恢复性激素浓度、精液参数和睾丸功能指数。结论:车前草饲粮可通过调节性激素、精子质量、睾丸功能指数和睾丸组织结构来预防阿特拉津所致大鼠睾丸毒性。
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引用次数: 0
Determination of the ovine ovarian reserve during the prenatal and neonatal periods 产前和新生儿期绵羊卵巢储备的测定
Q1 Veterinary Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380986
Isam B. Sharum
Objective: To determine the ovine ovarian histomorphology and follicular staging at various age periods in Awassi breed. Methods: Ovaries were collected from prenatal fetuses [gestational age (95±5) days], neonatal (day 0), and prepubertal ewe lambs (two and four months of age); each age group included six animals. Ovaries (n=12, each group) were dissected and processed for hematoxylin and eosin staining. Stained sections (n=24, each group) were imaged and utilized for histomorphology assessment, follicle measurement, and classification. Results: Prenatal ovaries were mainly enriched with primordial follicles accompanied by a lower proportion of primary follicles. In addition to primordial and primary follicles, neonatal ovaries demonstrated a proportion of centrally located multilayered and antral follicles. In comparison with neonatal ovaries, the proportion of multilayered and antral follicles was significantly higher in the ovaries of two-month-old lambs; conversely, the proportion of peripherally situated primordial follicles dramatically declined compared to that of earlier age of lamb. Although there was no statistical variation in the sizes of primordial follicles across groups, the mean diameter of the primary follicle in the prenatal ovaries was substantially smaller than in postnatal ovaries. Compared to the neonatal ovaries, the size of the multilayered and antral follicles in the prepubertal ovaries was substantially larger. Conclusions: The earliest follicular developmental stages were established prenatally whereas the advanced growth stages started in the neonatal period and greatly increased in the prepubertal period.
目的:了解Awassi不同年龄段绵羊卵巢组织形态及卵泡分期。方法:从产前胎儿[胎龄(95±5)天]、新生儿(0天)和青春期前母羊羔羊(2个月龄和4个月龄)采集卵巢;每个年龄组包括6只动物。解剖卵巢(每组n=12)并进行苏木精和伊红染色。对染色切片(每组n=24)进行成像,并用于组织形态学评估、毛囊测量和分类。结果:产前卵巢主要富含原始卵泡,初级卵泡比例较低。除了原始卵泡和初级卵泡外,新生儿卵巢还显示出一定比例的位于中心的多层和窦状卵泡。与新生儿卵巢相比,两个月大的羔羊卵巢中多层卵泡和窦状卵泡的比例明显更高;相反,与早期羔羊相比,位于外围的原始卵泡的比例显著下降。尽管各组原始卵泡的大小没有统计学差异,但产前卵巢中初级卵泡的平均直径明显小于产后卵巢。与新生儿卵巢相比,青春期前卵巢中多层和窦状卵泡的大小要大得多。结论:最早的卵泡发育阶段是在产前建立的,而晚期生长阶段始于新生儿期,并在青春期前大大增加。
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引用次数: 0
The barriers of joining in vitro fertilization programs among infertile couples in developing countries: A scoping review 发展中国家不孕夫妇参与体外受精项目的障碍:范围界定综述
Q1 Veterinary Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380981
A. Dewi, A. Wicaksana, Muhammad Lutfi, Agung Dewanto
Objective: To determine the barriers of joining in-vitro fertilization (IVF) programs among infertile couples in developing countries. Methods: This study assessed infertile couples and the barriers or associated factors resulting in delayed decision-making of joining IVF program by searching databases PubMed, ScienceDirect, EBSCO, ClinicalKey, and Cochrane Library from inception until December 31, 2021. Additional search strategies were snowballing literature search and citation tracking. Results: Eleven articles were included in the scoping review. The cost was the greatest barrier of joining IVF program. Limited access and lack of assisted reproductive technology centers, few qualified infertility trained staff, insufficient government support, low priority in government policy, along with sociocultural factors, such as religion and false beliefs or myths were also majorly considered to be associated obstacles. Conclusions: The main barrier associated with IVF program among infertile couples in developing countries is the high cost of the IVF services.
目的:确定发展中国家不孕夫妇参加体外受精(IVF)计划的障碍。方法:本研究通过检索PubMed、ScienceDirect、EBSCO、ClinicalKey和Cochrane Library数据库,从研究开始到2021年12月31日,评估不孕夫妇以及导致延迟加入IVF计划决策的障碍或相关因素。其他的搜索策略是滚雪球式的文献搜索和引文跟踪。结果:11篇文章被纳入范围审查。费用是加入体外受精计划的最大障碍。辅助生殖技术中心有限和缺乏,合格的不孕症培训人员很少,政府支持不足,政府政策不优先,以及宗教和错误信仰或神话等社会文化因素,也被认为是主要的相关障碍。结论:发展中国家不孕夫妇进行体外受精的主要障碍是体外受精服务的高成本。
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引用次数: 0
Effects of SARS-CoV-2 infection on pregnancy outcome: An overview 严重急性呼吸系统综合征冠状病毒2型感染对妊娠结局的影响:综述
Q1 Veterinary Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380982
Y. Amin
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may have harmful effects on expectant moms, labor, and newborns born to infected mothers. There is a risk of the initiation of pregnancy with infection. Even if the gametes are non-infected, pregnancies could be exposed later for infection by coronavirus. Investigations of COVID-19 during pregnancy highlighted the non-transmission or surprising transmission of SARS-CoV-2 to the offspring. However, other studies have exhibited the potential mother-to-fetus transmission. In this way, unanswered concerns about SARS-CoV-2 fetal transmission and the particular interface(s) controlling its pathogenesis throughout pregnancy persist. This review focuses on the potential effects of SARS-CoV-2 on vertical transmission, as well as the influence of the virus on pregnancy and placenta.
严重急性呼吸系统综合征冠状病毒2型感染可能对孕妇、分娩者和感染母亲所生的新生儿产生有害影响。有感染引发妊娠的风险。即使配子没有被感染,怀孕后也可能感染冠状病毒。对怀孕期间新冠肺炎的调查强调了SARS-CoV-2未传播或令人惊讶地传播给后代。然而,其他研究显示了潜在的母婴传播。通过这种方式,对严重急性呼吸系统综合征冠状病毒2型胎儿传播和控制其发病机制的特定界面的担忧在整个妊娠期持续存在。这篇综述的重点是严重急性呼吸系统综合征冠状病毒2型对垂直传播的潜在影响,以及该病毒对妊娠和胎盘的影响。
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引用次数: 0
Oxidized palm oil impairs reproductive functions and architectures in female rats 氧化棕榈油损害雌性大鼠的生殖功能和结构
Q1 Veterinary Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380985
Wankeu-Nya Modeste, Inès Kengne, Benjamin Ateba, Fort Bend, Ornela Djeumeni, Dominique Hatho, E. Ngadjui, P. Moundipa, D. Massoma, A. Dongmo, Watcho Pierre
Objective: To evaluate the effects of three oxidized palm oil diets (OPD) on female rat reproductive function. Methods: Forty-four female Wistar rats presenting five consecutive and regular estrous cycles were divided into 4 groups. The rats were fed with: a standard diet, 70% of standard diet+30% oxidized palm oil diet (OPD1), OPD1+5 g of boiled yolk egg (OPD2) and OPD1+10% sucrose (OPD3) for 125 days, respectively. During the feeding period, morphometric, estrous cycle, sexual behavior, gestation, biochemical and histomorphometric parameters were evaluated. Results: All OPDs significantly increased abdominal circumference, body mass index and Lee index coupled to an irregularity and lengthening of the estrous cycle. They significantly decreased appetite and consumption behaviours, quantic pregnancy index, fertility rate, implantation sites and index, serum progesterone and high-density lipoprotein levels, increased pre-implantation losses, anti-implantation activities, serum estradiol, triglycerides, total and low-density lipoprotein-cholesterol levels, and impaired brain and ovaries oxidative status. Histomorphometric examinations revealed increases in the number of atresic and primary follicles and decreases in secondary, tertiary, Degraaf, total and corpus luteum follicles in ovaries coupled to a neurodegeneration of hypothalamic anteroventral periventricular neurons in the OPD groups compared to the standard diet group. Conclusions: The three OPDs induce obesity and impair the female reproductive function, especially OPD2 and OPD3. These findings contribute to a better understanding of the adverse effects of palm oil bleaching on the reproductive function in female rats, which could be useful in the management of women with obesity-related sexual dysfunction.
目的:评价3种氧化棕榈油日粮(OPD)对雌性大鼠生殖功能的影响。方法:44只雌性Wistar大鼠连续5个规律的发情周期,随机分为4组。分别饲喂标准日粮、70%标准日粮+30%氧化棕榈油日粮(OPD1)、OPD1+5 g水煮蛋黄蛋(OPD2)和OPD1+10%蔗糖(OPD3) 125 d。在摄食期间,进行形态计量、发情周期、性行为、妊娠、生化及组织形态计量等指标的测定。结果:所有opd均显著增加了小鼠的腹围、体重指数和Lee指数,并伴有发情周期的不规则和延长。他们的食欲和消费行为、定量妊娠指数、生育率、着床部位和指数、血清黄体酮和高密度脂蛋白水平显著降低,着床前损失、抗着床活性、血清雌二醇、甘油三酯、总脂蛋白和低密度脂蛋白-胆固醇水平升高,脑和卵巢氧化状态受损。组织形态学检查显示,与标准饮食组相比,OPD组卵巢中闭锁和初级卵泡数量增加,第二、第三、Degraaf、总卵泡和黄体卵泡数量减少,并伴有下丘脑腹侧脑室周围神经元的神经变性。结论:3种opd诱导肥胖,损害女性生殖功能,以OPD2和OPD3最为明显。这些发现有助于更好地了解棕榈油漂白对雌性大鼠生殖功能的不利影响,这可能对肥胖相关性功能障碍女性的管理有用。
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引用次数: 0
Pumpkin seed ethanolic extract protects against escitalopram-induced reproductive toxicity in male mice 南瓜籽乙醇提取物对艾司西酞普兰诱导的雄性小鼠生殖毒性的保护作用
Q1 Veterinary Pub Date : 2023-03-01 DOI: 10.4103/2305-0500.372377
Agrawal Karuna, Shahani Lata, B. Pradeep
Objective: To investigate the protective role of pumpkin seed ethanolic extract against escitalopram-induced reproductive toxicity in male mice. Methods: Swiss albino male mice were randomly divided into five groups with six mice in each group. Group I received normal water orally, Group II, III, IV and V received escitalopram oxalate (10 mg/kg), pumpkin seed extract (300 mg/kg) plus escitalopram oxalate (10 mg/kg), escitalopram oxalate (20 mg/kg), and pumpkin seed extract (300 mg/kg) plus escitalopram oxalate (20 mg/kg), respectively. All test doses were continuously administered orally once daily per animal body weight for 30 days and 60 days. Body weight and sexual organ weight were evaluated on day 31 and 61. Effects of pumpkin seed extract on sperm parameters, biochemical parameters and histology of testis were also investigated. Results: Escitalopram 10 or 20 mg/kg caused reproductive toxicity in male mice after 30 and 60 days of treatment. However, simultaneous administration of escitalopram oxalate (10 or 20 mg/kg) with pumpkin seed extract (300 mg/kg) attenuated escitalopram-induced testicular toxicity. Significant increase in the body weight and relative organ weight was observed. Sperm count, sperm motility and viability significantly increased (P<0.05). The histopathological alterations caused by escitalopram was also ameliorated. Conclusions: Ethanolic extract of pumpkin seeds (300 mg/kg body weight) protects again reproductive toxicity induced by escitalopram. Therefore, dietary intake of pumpkin seed extract might be useful for male patients who expose to antidepressant drug due to depression.
目的:研究南瓜子乙醇提取物对艾司西酞普兰雄性小鼠生殖毒性的保护作用。方法:将瑞士白化病雄性小鼠随机分为5组,每组6只。第一组口服正常水,第二组、第三组、第四组和第五组分别接受草酸艾西酞普兰(10 mg/kg)、南瓜籽提取物(300 mg/kg)加草酸艾西他普兰(10 mg/kg)、草酸艾西特普兰(20 mg/kg)和南瓜籽提取物。所有试验剂量连续口服,每只动物体重每天一次,持续30天和60天。在第31天和第61天评估体重和性器官重量。研究了南瓜籽提取物对精子参数、睾丸生化参数和组织学的影响。结果:依西酞普兰10或20mg/kg给药30和60天后对雄性小鼠产生生殖毒性。然而,草酸艾司西酞普兰(10或20 mg/kg)与南瓜籽提取物(300 mg/kg)同时给药可减轻艾司西普兰诱导的睾丸毒性。观察到体重和相对器官重量显著增加。精子计数、精子活力和活力显著增加(P<0.05),艾司西酞普兰引起的组织病理学改变也得到改善。结论:南瓜籽乙醇提取物(300mg/kg体重)可再次保护艾司西酞普兰引起的生殖毒性。因此,从饮食中摄入南瓜籽提取物可能对因抑郁症而接触抗抑郁药物的男性患者有用。
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引用次数: 0
Effect of heparin on recurrent IVF-ET failure patients 肝素对复发性IVF-ET失败患者的影响
Q1 Veterinary Pub Date : 2023-03-01 DOI: 10.4103/2305-0500.372376
N. Ghasemi, Maryam Shirmohamadi, M. Mashayekhy, Iraj Alipourfard, Javad Fazeli
Objective: To elucidate the possible role of unfractionated heparin in patients with failed repeated in in vitro fertilization and embryo transfer (IVF-ET) and thrombophilia. Methods: This case control study evaluated the efficacy of the unfractionated heparin in increasing the pregnancy and implantation ratio in women with recurrent IVF-ET failures. Eighty-six women received in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) with a record of three or more previous IVF-ET failures. Participants were randomly distributed into two groups. Group A (n=43) received unfractionated heparin 5 000 IU twice daily, and group B (n=43) did not take any antithrombotic drugs. Coagulation abnormalities such as factor V Leiden (FVL) mutation, methylene tetra hydro folate reductase (MTHFR) mutation and prothrombin mutation (FII) were evaluated. Age, body mass index, basal follicular stimulating hormone, basal estradiol, duration of infertility, and number of IVF-ET failures were compared between two groups. Results: 45.0% and 17.4% of women were pregnant with and without MTHFR and prothrombin mutation, respectively, when they received unfractionated heparin treatment. The implantation rate was more in group A (12.5%) than group B (4.3%) and differences in the fertilization rate of the two groups were observed (27.7% vs. 35.9%). The clinical pregnancy rate per cycle was remarkably more in group A (30.2%) than group B (14.0%). Conclusions: Heparin is a safe and valuable treatment for patients with repeated IVF-ET failures. The clinical pregnancy and implantation rates are higher in the heparin-treated group in contrast with the control group. Trial registration: The trial registration was done with clinical registration number of “ IRCT138807202575N1”.
目的:探讨未分离肝素在体外受精和胚胎移植(IVF-ET)失败及血栓形成患者中的作用。方法:本病例对照研究评价未分级肝素对提高IVF-ET复发失败妇女妊娠和着床率的疗效。86名接受体外受精/胞浆内单精子注射(IVF/ICSI)的妇女有3次或3次以上IVF- et失败的记录。参与者被随机分为两组。A组(n=43)每日2次给予未分离肝素5 000 IU, B组(n=43)未服用任何抗血栓药物。评估凝血异常,如V - Leiden因子(FVL)突变、亚甲基四氢叶酸还原酶(MTHFR)突变和凝血酶原突变(FII)。比较两组患者的年龄、体重指数、基础卵泡刺激素、基础雌二醇、不孕持续时间和IVF-ET失败次数。结果:接受肝素单次治疗时,MTHFR和凝血酶原突变的孕妇分别占45.0%和17.4%。A组着床率(12.5%)高于B组(4.3%),两组受精率差异(27.7% vs. 35.9%)。A组临床单周期妊娠率(30.2%)明显高于B组(14.0%)。结论:肝素对于反复IVF-ET失败患者是一种安全且有价值的治疗方法。肝素治疗组临床妊娠和着床率均高于对照组。试验注册:临床注册号为“IRCT138807202575N1”。
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引用次数: 0
期刊
Asian Pacific Journal of Reproduction
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