Solid state fermentation for a red pigment production of Monascus ruber NBRC 32318 on rice in an aerated packed bed bioreactor was studied. The constant and the inconstant aeration rates were studied in this research. The constant rates were performed using 0.05 and 0.10 vvm during fermentation. The inconstant ones were conducted by changing the aeration rate when the growth of fungi entered the stationary phase. The inconstant aeration were studied in three different modes composed of 1) starting with 0.10 vvm aeration and then changing to 0.05 vvm one, 2) starting with 0.10 vvm aeration and then increasing to 0.15 vvm one, and 3) starting with 0.05 vvm aeration and then increasing to 0.10 vvm one. The growth and the density of red pigment were analyzed along the height of packed bed. The results show the effect of changing aeration rate during fermentation on the red pigment production. The appropriated aeration rate seemed to be relative with the height of bed.
{"title":"Effect of aeration mode on the red pigment production by monascus buber on rice during fermenting in the packed bed bioreactor","authors":"T. Chysirichote","doi":"10.14456/KKURJ.2016.13","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.13","url":null,"abstract":"Solid state fermentation for a red pigment production of Monascus ruber NBRC 32318 on rice in an aerated packed bed bioreactor was studied. The constant and the inconstant aeration rates were studied in this research. The constant rates were performed using 0.05 and 0.10 vvm during fermentation. The inconstant ones were conducted by changing the aeration rate when the growth of fungi entered the stationary phase. The inconstant aeration were studied in three different modes composed of 1) starting with 0.10 vvm aeration and then changing to 0.05 vvm one, 2) starting with 0.10 vvm aeration and then increasing to 0.15 vvm one, and 3) starting with 0.05 vvm aeration and then increasing to 0.10 vvm one. The growth and the density of red pigment were analyzed along the height of packed bed. The results show the effect of changing aeration rate during fermentation on the red pigment production. The appropriated aeration rate seemed to be relative with the height of bed.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"176-184"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Production of exobiopolymers by Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694, interleukin-8 (IL-8) inducers, were optimized using experimental design. Biological and physiological properties of these exobiopolymers are attractive to use as wound-dressing material, so that it is very interesting to increase its production. Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694 were cultivated on different carbon and nitrogen sources and the best carbon and nitrogen sources for exobiopolymer production of Phytocordyceps sp. BCC 2744 were glucose and peptone, respectively, and 0.88 g/L exobiopolymer was obtained. Higher exobiopolymer (1.82 g/L) was obtained on glucose and meat extract by Akanthomyces pistillariiformis BCC 2694. After the effects of 4 variables were studied using a two-level fractional design, glucose and peptone concentration were the most influential parameters on exobiopolymer production of Phytocordyceps sp. BCC 2744. Lower exobiopolymer production was obtained in the medium supplemented with 5-Fluorouracil and vitamin solution at high level (10 mM and 3mL/L, respectively). The highest exobiopolymer production was obtained on 60 g/L glucose and 20 g/L peptone and 2.32 g/L exobiopolymer was produced. About 4.0 g/L exobiopolymer production in a 20 L bioreactor was obtained. At least 4 variables; medium type, nitrogen sources, glucose, and nitrogen concentration were applied on a two-level factorial design of biomass and exobiopolymer production of Akanthomyces pistillariiformis BCC 2694. Models obtained from the multiple regression are significant. At high level of glucose (60 g/L), phosphate medium, and 20 g/L meat extract, higher exobiopolymer production and about 2.54 g/L was obtained. About 4.5 g/L exobiopolymer production in 20 L bioreactor was obtained.
{"title":"Exobiopolymer production of Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694; optimization and scale-up","authors":"W. Prathumpai, P. Rachtawee","doi":"10.14456/KKURJ.2016.24","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.24","url":null,"abstract":"Production of exobiopolymers by Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694, interleukin-8 (IL-8) inducers, were optimized using experimental design. Biological and physiological properties of these exobiopolymers are attractive to use as wound-dressing material, so that it is very interesting to increase its production. Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694 were cultivated on different carbon and nitrogen sources and the best carbon and nitrogen sources for exobiopolymer production of Phytocordyceps sp. BCC 2744 were glucose and peptone, respectively, and 0.88 g/L exobiopolymer was obtained. Higher exobiopolymer (1.82 g/L) was obtained on glucose and meat extract by Akanthomyces pistillariiformis BCC 2694. After the effects of 4 variables were studied using a two-level fractional design, glucose and peptone concentration were the most influential parameters on exobiopolymer production of Phytocordyceps sp. BCC 2744. Lower exobiopolymer production was obtained in the medium supplemented with 5-Fluorouracil and vitamin solution at high level (10 mM and 3mL/L, respectively). The highest exobiopolymer production was obtained on 60 g/L glucose and 20 g/L peptone and 2.32 g/L exobiopolymer was produced. About 4.0 g/L exobiopolymer production in a 20 L bioreactor was obtained. At least 4 variables; medium type, nitrogen sources, glucose, and nitrogen concentration were applied on a two-level factorial design of biomass and exobiopolymer production of Akanthomyces pistillariiformis BCC 2694. Models obtained from the multiple regression are significant. At high level of glucose (60 g/L), phosphate medium, and 20 g/L meat extract, higher exobiopolymer production and about 2.54 g/L was obtained. About 4.5 g/L exobiopolymer production in 20 L bioreactor was obtained.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"68-80"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Papaya is a popular tropical fruit that is widely consumed in Thailand. In this study, fresh papaya fruit was subjected to vacuum impregnation to have a better understanding about the process parameters, including impregnation solution ratio, impregnation time and relaxation time. The fresh fruit was cut into pieces, added to impregnation solutions at ratios of 1:5 or 1:10, vacuum impregnated at 50 mbar for 5 or 10 min and left for another 10 or 30 min in the impregnation solution. After separating the fruit from the solution, it was analyzed for the fruit physicochemical properties, including real porosity (e r ), volume of fruit impregnated with an external solution (X value), fruit volume deformation (γ value), effective porosity (e e ), water loss and solid gain. Different factors investigated in this study significantly affected vacuum impregnated parameters of papaya pieces (p<0.05). The papaya treatment in the impregnation solution at 1:10 with 10 min vacuum time and 30 min relaxation time significantly produced the highest solid gain (3.36 ± 0.37%), X value (0.24 ± 0.01 m 3 liquid/m 3 sample), γ value (0.14 ± 0.03 m 3 /m 3 initial sample) and e e value (0.11 ± 0.05%). At the same time, this particular papaya sample possessed the lowest water loss (–15.22 ± 3.65%) and e r value (0.16 ± 0.01%). Data in this study strongly indicated higher impregnation solution ratio with longer impregnation and relaxation periods produced better infusion of impregnation solution in papaya pieces.
木瓜是一种受欢迎的热带水果，在泰国广泛食用。本研究对新鲜番木瓜果实进行真空浸渍，以了解浸渍液比、浸渍时间和松弛时间等工艺参数。将新鲜水果切成块，按1:5或1:10的比例加入浸渍液中，在50mbar下真空浸渍5或10分钟，然后在浸渍液中再浸泡10或30分钟。将果实与溶液分离后，分析果实的物理化学性质，包括实际孔隙率(e r)、浸渍后果实的体积(X值)、果实体积变形(γ值)、有效孔隙率(e e)、失水和固重。不同因素对真空浸渍木瓜片的工艺参数有显著影响(p<0.05)。在1:10的浸渍液中，真空时间为10 min，松弛时间为30 min，木瓜的固相增益为3.36±0.37%，X值为0.24±0.01 m 3 /m 3样品，γ值为0.14±0.03 m 3 /m 3初始样品，e - e值为0.11±0.05%。同时，该木瓜样品具有最低的失水(-15.22±3.65%)和e - r值(0.16±0.01%)。本研究的数据强烈表明，较高的浸渍液比、较长的浸渍时间和松弛时间使浸渍液在木瓜片中的浸渍效果更好。
{"title":"Effect of impregnation solution ratio and periods on vacuum impregnated papaya","authors":"N. Benyakart, A. Phianmongkhol, T. Wirjantoro","doi":"10.14456/kkurj.2016.54","DOIUrl":"https://doi.org/10.14456/kkurj.2016.54","url":null,"abstract":"Papaya is a popular tropical fruit that is widely consumed in Thailand. In this study, fresh papaya fruit was subjected to vacuum impregnation to have a better understanding about the process parameters, including impregnation solution ratio, impregnation time and relaxation time. The fresh fruit was cut into pieces, added to impregnation solutions at ratios of 1:5 or 1:10, vacuum impregnated at 50 mbar for 5 or 10 min and left for another 10 or 30 min in the impregnation solution. After separating the fruit from the solution, it was analyzed for the fruit physicochemical properties, including real porosity (e r ), volume of fruit impregnated with an external solution (X value), fruit volume deformation (γ value), effective porosity (e e ), water loss and solid gain. Different factors investigated in this study significantly affected vacuum impregnated parameters of papaya pieces (p<0.05). The papaya treatment in the impregnation solution at 1:10 with 10 min vacuum time and 30 min relaxation time significantly produced the highest solid gain (3.36 ± 0.37%), X value (0.24 ± 0.01 m 3 liquid/m 3 sample), γ value (0.14 ± 0.03 m 3 /m 3 initial sample) and e e value (0.11 ± 0.05%). At the same time, this particular papaya sample possessed the lowest water loss (–15.22 ± 3.65%) and e r value (0.16 ± 0.01%). Data in this study strongly indicated higher impregnation solution ratio with longer impregnation and relaxation periods produced better infusion of impregnation solution in papaya pieces.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"291-298"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Green algae are able to convert the unlimited sunlight energy to produce hydrogen via photosynthesis. In seawater, several kinds of m arine microalgae are widespread and abundant and have been shown to tolerate and survive under the extreme salt concentrations . This work aimed to study the screening of high H 2 producing marine green algal strains isolated from the Gulf of Thailand and the Andaman Sea, and the selection of the highest H 2 producing strain. Its H 2 production was investigated under photoheterotrophic cultivation. The result revealed that among 20 marine green algal strains, the green alga Chlorella sp. LSD-W2 gave the highest H 2 production rate in both light and dark anaerobic conditions. During photoheterotrophic cultivation Chlorella sp. LSD-W2 was rapidly grown in TAP (Tris-Acetate-Phosphate) medium and reached the stationary growth phase after 36 h of cultivation. The highest photohydrogen production rate was found in cells incubated in NH 4 Cl-deprived TAP medium. It was approximately 20-fold higher than H 2 production rate of cells in a normal TAP medium.
{"title":"Hydrogen production by unicellular green alga chlorella sp. LSD-W2 isolated from seawater in Thailand","authors":"N. Tinpranee, A. Incharoensakdi, S. Phunpruch","doi":"10.14456/KKURJ.2016.32","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.32","url":null,"abstract":"Green algae are able to convert the unlimited sunlight energy to produce hydrogen via photosynthesis. In seawater, several kinds of m arine microalgae are widespread and abundant and have been shown to tolerate and survive under the extreme salt concentrations . This work aimed to study the screening of high H 2 producing marine green algal strains isolated from the Gulf of Thailand and the Andaman Sea, and the selection of the highest H 2 producing strain. Its H 2 production was investigated under photoheterotrophic cultivation. The result revealed that among 20 marine green algal strains, the green alga Chlorella sp. LSD-W2 gave the highest H 2 production rate in both light and dark anaerobic conditions. During photoheterotrophic cultivation Chlorella sp. LSD-W2 was rapidly grown in TAP (Tris-Acetate-Phosphate) medium and reached the stationary growth phase after 36 h of cultivation. The highest photohydrogen production rate was found in cells incubated in NH 4 Cl-deprived TAP medium. It was approximately 20-fold higher than H 2 production rate of cells in a normal TAP medium.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"256-266"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Nuwan, P. Piwpan, Adisak Jaturapiree, P. Jaturapiree
Dextran is a natural homopolysaccharide composed of α (1,6)-linkages in their major chain and is synthesized by dextransucrase of the microbial cell in the presence of sucrose as a substrate. The production of dextran by sucrose fermentation using Leuconostoc mesenteroides TISTR 053 was carried out in a Biostat B plus fermenter with total working volume of 3 liter. The effects of sucrose concentrations and modes of operation (batch and fed batch) were studied. The maximum dextran production obtained after 24 hours of incubation at 37 o C with 20% sucrose in batch fermentation. The fed batch fermentation promoted the dextran productivity. The structure of dextran was determined and confirmed by FTIR and NMR.
右旋糖酐是一种由α(1,6)键为主链组成的天然均多糖，由微生物细胞的右旋糖酐酶在蔗糖作为底物的存在下合成。在总工作容积为3升的Biostat B +发酵罐中，利用肠系膜Leuconostoc肠系膜菌TISTR 053进行蔗糖发酵生产葡聚糖。研究了不同的蔗糖浓度和不同的操作方式(分批和进料分批)对发酵效果的影响。在37℃和20%蔗糖的条件下分批发酵24小时后葡聚糖产量最大。分批补料发酵提高了葡聚糖的产率。用FTIR和NMR测定了葡聚糖的结构。
{"title":"Production of dextran by leuconostoc mesenteroides TISTR 053 in fed batch fermentation","authors":"P. Nuwan, P. Piwpan, Adisak Jaturapiree, P. Jaturapiree","doi":"10.14456/KKURJ.2016.45","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.45","url":null,"abstract":"Dextran is a natural homopolysaccharide composed of α (1,6)-linkages in their major chain and is synthesized by dextransucrase of the microbial cell in the presence of sucrose as a substrate. The production of dextran by sucrose fermentation using Leuconostoc mesenteroides TISTR 053 was carried out in a Biostat B plus fermenter with total working volume of 3 liter. The effects of sucrose concentrations and modes of operation (batch and fed batch) were studied. The maximum dextran production obtained after 24 hours of incubation at 37 o C with 20% sucrose in batch fermentation. The fed batch fermentation promoted the dextran productivity. The structure of dextran was determined and confirmed by FTIR and NMR.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"366-375"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fructosyltransferases (FTases) were important enzymes for fructo-oligosaccharides (FOS) synthesis. Recently, FOS was considered as a potential prebiotic in food industry. In particularly, FOS with short chains was used as a new alternative sweetener. The production of recombinant 1- fructan: fructan fructosyltransferase (1- FFT) in yeast system was conducted in this research. The 1- fructan: fructan fructosyltransferase gene (1- fft) cloned from 105 days old tuber of Kaentawan ( Helianthus tuberosus L.) was sub cloned to expression vector, pPICZαB by adding Pst I and Sac II sites. The cloned gene was successfully transformed to yeast Pichia pastoris X-33 by lithium chloride transformation method. The yeast transformant; P. pastoris X- 33 PF1 showed the ability to produce recombinant 1- FFT. The enzyme activity at 3.57 and 3.33 unit/L was determined in cell and culture medium, respectively. It was also found that FOS was synthesized when recombinant 1- FFT was incubated with 1- kestose and synthesized FOS as substrates. The synthesis of FOS was not detected when sucrose was used as substrate of recombinant 1- FFT.
{"title":"Cloning of 1– fructan: fructan fructosyltransferase gene and expression of recombinant 1– fructan: fructan fructosyltransferase in yeast","authors":"B. Ngampanya, Kriengsak Boonchoo","doi":"10.14456/KKURJ.2016.46","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.46","url":null,"abstract":"Fructosyltransferases (FTases) were important enzymes for fructo-oligosaccharides (FOS) synthesis. Recently, FOS was considered as a potential prebiotic in food industry. In particularly, FOS with short chains was used as a new alternative sweetener. The production of recombinant 1- fructan: fructan fructosyltransferase (1- FFT) in yeast system was conducted in this research. The 1- fructan: fructan fructosyltransferase gene (1- fft) cloned from 105 days old tuber of Kaentawan ( Helianthus tuberosus L.) was sub cloned to expression vector, pPICZαB by adding Pst I and Sac II sites. The cloned gene was successfully transformed to yeast Pichia pastoris X-33 by lithium chloride transformation method. The yeast transformant; P. pastoris X- 33 PF1 showed the ability to produce recombinant 1- FFT. The enzyme activity at 3.57 and 3.33 unit/L was determined in cell and culture medium, respectively. It was also found that FOS was synthesized when recombinant 1- FFT was incubated with 1- kestose and synthesized FOS as substrates. The synthesis of FOS was not detected when sucrose was used as substrate of recombinant 1- FFT.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"356-365"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66677005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The primary objective of this study was to analyze some chemical hazards, develop a HACCP plan and its validation to ensure a safety system for production of instant black rice beverage. Chemical hazards in food and beverage produced from cereal are mostly from natural occurrence . Lead and aflatoxins, which could be produced by toxigenic molds associated with black rice and emerged at each stage of the production was described. Knowing of product characterization, raw materials, ingredients, and the production flow diagram are the keys to develop a HACCP plan. This plan was developed the execution of hazard analysis (HA), identifying the critical control points (CCPs), establishment of critical limits (CLs), monitoring procedure, establishment of corrective action, establishment of verification procedure and establishment of record keeping. The validation of HACCP plan was based on the safety hazard testing, scientific publication and the regulatory documents. Status of the control measures and effectiveness to ensure the chemical safety of instant black rice beverage were discussed.
{"title":"Chemical hazards associated with instant black rice beverage","authors":"Hour Phann, J. Uriyapongson, Alli Inteaz","doi":"10.14456/kkurj.2016.47","DOIUrl":"https://doi.org/10.14456/kkurj.2016.47","url":null,"abstract":"The primary objective of this study was to analyze some chemical hazards, develop a HACCP plan and its validation to ensure a safety system for production of instant black rice beverage. Chemical hazards in food and beverage produced from cereal are mostly from natural occurrence . Lead and aflatoxins, which could be produced by toxigenic molds associated with black rice and emerged at each stage of the production was described. Knowing of product characterization, raw materials, ingredients, and the production flow diagram are the keys to develop a HACCP plan. This plan was developed the execution of hazard analysis (HA), identifying the critical control points (CCPs), establishment of critical limits (CLs), monitoring procedure, establishment of corrective action, establishment of verification procedure and establishment of record keeping. The validation of HACCP plan was based on the safety hazard testing, scientific publication and the regulatory documents. Status of the control measures and effectiveness to ensure the chemical safety of instant black rice beverage were discussed.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"143 1","pages":"336-346"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66677020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wimada Srisuwan, Charin Techapun, Phisit Seesuriyachan, M. Watanabe, Thanongsak Chaiyaso
Rice residue from food waste contained of starch as a mainly component which could be either hydrolyzed to be fermentable sugars or directly used as a carbon source for the growth and high value metabolites production by various microorganisms. Therefore, this study focused on the utilization of rice residue and rice residue hydrolysate from food waste as a carbon source for the growth and lipids production of oleaginous yeast. Rice residue obtained from canteen of the Faculty of Agro-Industry, Chiang Mai University, Thailand. It composed of moisture content (76.68±0.55%), crude fat (1.76±0.47%), crude protein (3.04±0.06%), ash content (0.46±0.07%), and carbohydrate content (18.05±0.01%), respectively. Rice residue was then subjected to enzymatic hydrolysis using α-amylase and amyloglucosidase (AMG), resulting the maximal reducing sugars of 168.02±0.02 g/L. The screening of oleaginous yeast from flowers and leaves samples from Doi-Inthanon National Park, Faculty of Agro-Industry, Chiang Mai University, the culture collection of the Thailand Institute of Scientific and Technological Research (TISTR) and the Division of Biotechnology, Faculty of Agro-Industry, Chiang Mai University were investigated. Sixty-seven isolates were obtained, and only four isolates were identified as oleaginous yeast because of containing high lipids content more than 20% (w/w), when glucose or rice residue hydrolysate was used as a carbon source. Those oleaginous yeasts were identified as Rhodotorula sp. C7, Rhodosporidium paludigenum C10, and the new isolate TC32, respectively. Their growths and lipid productions were compared with Diozegia sp. TISTR5792. The results showed that, C7, C10, TISTR5792 and TC32 produced the maximal lipids content of 24.26±0.56, 23.69±0.91, 22.43±1.09 and 23.07±0.80% (w/w) when cultivated in the basal medium supplemented with enzymatic-rice residue hydrolysate. Surprisingly, we found that TISTR5792 and TC32 could grow well in the medium supplemented with rice residue "> (without hydrolysis) and showed lipids content of 18.41±0.10 and 21.67±0.02% (w/w), respectively. These results indicated that rice residue from food waste shows a high potential to be an effective carbon source for the growth and lipid production of the selected oleaginous yeasts.
{"title":"Screening of oleaginous yeast for lipid production using rice residue from food waste as a carbon source","authors":"Wimada Srisuwan, Charin Techapun, Phisit Seesuriyachan, M. Watanabe, Thanongsak Chaiyaso","doi":"10.14456/KKURJ.2016.19","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.19","url":null,"abstract":"Rice residue from food waste contained of starch as a mainly component which could be either hydrolyzed to be fermentable sugars or directly used as a carbon source for the growth and high value metabolites production by various microorganisms. Therefore, this study focused on the utilization of rice residue and rice residue hydrolysate from food waste as a carbon source for the growth and lipids production of oleaginous yeast. Rice residue obtained from canteen of the Faculty of Agro-Industry, Chiang Mai University, Thailand. It composed of moisture content (76.68±0.55%), crude fat (1.76±0.47%), crude protein (3.04±0.06%), ash content (0.46±0.07%), and carbohydrate content (18.05±0.01%), respectively. Rice residue was then subjected to enzymatic hydrolysis using α-amylase and amyloglucosidase (AMG), resulting the maximal reducing sugars of 168.02±0.02 g/L. The screening of oleaginous yeast from flowers and leaves samples from Doi-Inthanon National Park, Faculty of Agro-Industry, Chiang Mai University, the culture collection of the Thailand Institute of Scientific and Technological Research (TISTR) and the Division of Biotechnology, Faculty of Agro-Industry, Chiang Mai University were investigated. Sixty-seven isolates were obtained, and only four isolates were identified as oleaginous yeast because of containing high lipids content more than 20% (w/w), when glucose or rice residue hydrolysate was used as a carbon source. Those oleaginous yeasts were identified as Rhodotorula sp. C7, Rhodosporidium paludigenum C10, and the new isolate TC32, respectively. Their growths and lipid productions were compared with Diozegia sp. TISTR5792. The results showed that, C7, C10, TISTR5792 and TC32 produced the maximal lipids content of 24.26±0.56, 23.69±0.91, 22.43±1.09 and 23.07±0.80% (w/w) when cultivated in the basal medium supplemented with enzymatic-rice residue hydrolysate. Surprisingly, we found that TISTR5792 and TC32 could grow well in the medium supplemented with rice residue \"> (without hydrolysis) and showed lipids content of 18.41±0.10 and 21.67±0.02% (w/w), respectively. These results indicated that rice residue from food waste shows a high potential to be an effective carbon source for the growth and lipid production of the selected oleaginous yeasts.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"116-126"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of diets containing various levels (0, 1, 3 and 5%) of waterlily (Nymphaea pubescens) stamen extract (NPSE) on growth performance and intestinal morphology were investigated in common lowland frog (Rana rugulosa) with an initial weight of 16.09±0.50 g. After 11 weeks of feeding, growth parameters were evaluated. The results showed that the frogs fed with diets supplemented with 3 and 5% of NPSE exhibited significantly higher weight gain, specific growth rate and feed conversion ratio than frogs fed diets supplemented with 0 and 1% of NPSE (P 0.05). Villi heights, villi widths and the thickness of intestinal muscle layers in posterior intestine were significantly increased in frogs fed the diets containing NPSE (P 0.05). Feeding behavior and feed acceptability of the experimental groups were the same as the control group. The optimal levels of NPSE observed in this present study were ranged between 3-5%. Our findings suggest that NPSE can be applied in the diets as a growth promoter in common lowland frog.
{"title":"Effects of dietary waterlily (Nymphaea pubescens) stamen extract on growth performance and intestinal morphology of common lowland frog (Rana rugulosa)","authors":"Wannapa Kamatit, S. Aoki, P. Munglue","doi":"10.14456/KKURJ.2016.28","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.28","url":null,"abstract":"The effects of diets containing various levels (0, 1, 3 and 5%) of waterlily (Nymphaea pubescens) stamen extract (NPSE) on growth performance and intestinal morphology were investigated in common lowland frog (Rana rugulosa) with an initial weight of 16.09±0.50 g. After 11 weeks of feeding, growth parameters were evaluated. The results showed that the frogs fed with diets supplemented with 3 and 5% of NPSE exhibited significantly higher weight gain, specific growth rate and feed conversion ratio than frogs fed diets supplemented with 0 and 1% of NPSE (P 0.05). Villi heights, villi widths and the thickness of intestinal muscle layers in posterior intestine were significantly increased in frogs fed the diets containing NPSE (P 0.05). Feeding behavior and feed acceptability of the experimental groups were the same as the control group. The optimal levels of NPSE observed in this present study were ranged between 3-5%. Our findings suggest that NPSE can be applied in the diets as a growth promoter in common lowland frog.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"31 1","pages":"30-41"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The heparinase-producing bacteria was isolated from brackish sediment and identified by morphological characteristic and 16S rRNA gene. The nucleotide sequence of 16S rRNA indicated highest similarity (97%-100%) with genus Aeromonas . The isolate was subsequently described as Aeromonas sp. RYA_En1. The crude intracellular enzyme produced by Aeromonas sp. RYA_En1 was partial purified by ammonium sulphate, ion exchange and gel filtration column chromatography, respectively. The active fraction obtained from gel filtration chromatography yielded enzyme production and enzyme specific activity of 410 U/L and 0.63 U/mg protein, respectively. Then, the purified fraction was determined for the optimal temperature and pH for the enzyme activities which were at 37 ° C and pH of 7.0, respectively. In addition, the molecular weight of the purified enzyme determined by the SDS-PAGE was approximately 90 kDa.
{"title":"Purification and Characterization of Heparin Degrading Enzyme by isolated bacteria from brackish sediment","authors":"Prapasri Khanitchadecha, W. Pulsawat","doi":"10.14456/KKURJ.2016.40","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.40","url":null,"abstract":"The heparinase-producing bacteria was isolated from brackish sediment and identified by morphological characteristic and 16S rRNA gene. The nucleotide sequence of 16S rRNA indicated highest similarity (97%-100%) with genus Aeromonas . The isolate was subsequently described as Aeromonas sp. RYA_En1. The crude intracellular enzyme produced by Aeromonas sp. RYA_En1 was partial purified by ammonium sulphate, ion exchange and gel filtration column chromatography, respectively. The active fraction obtained from gel filtration chromatography yielded enzyme production and enzyme specific activity of 410 U/L and 0.63 U/mg protein, respectively. Then, the purified fraction was determined for the optimal temperature and pH for the enzyme activities which were at 37 ° C and pH of 7.0, respectively. In addition, the molecular weight of the purified enzyme determined by the SDS-PAGE was approximately 90 kDa.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"411-419"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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