Rice husk, a low cost carbon sources from agricultural waste was used for PHAs production. However, the structural complexity of lignocellulosic materials in rice husk requires a pretreatment step prior used as carbon source. The effect of the main operating factors including sulfuric acid concentration and hydrolysis temperature on fermentable sugar production from rice husk were investigated by response surface methodology. The results indicated that total sugar and reducing sugar concentration ranged between 12.36 and 30.12 g/L, and 0.28 and 6.66 g/L, respectively. The satisfactory condition of 9.20 % w/v sulfuric acid at 107.86 0 C was established for reducing sugar and total sugar productions. Furthermore, the non-detoxified rice husk hydrolysate was then used as a carbon source for 10 isolate microbial strains to test PHA production capability. It was found that the highest biomass was observed as 1.01g/l and PHA content as 11.2 %.
{"title":"Polyhydroxyalkanoate (PHA) synthesis by newly bacterial isolates using non-detoxified rice husk hydrolysate","authors":"V. Tanamool, W. Soemphol","doi":"10.14456/KKURJ.2016.41","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.41","url":null,"abstract":"Rice husk, a low cost carbon sources from agricultural waste was used for PHAs production. However, the structural complexity of lignocellulosic materials in rice husk requires a pretreatment step prior used as carbon source. The effect of the main operating factors including sulfuric acid concentration and hydrolysis temperature on fermentable sugar production from rice husk were investigated by response surface methodology. The results indicated that total sugar and reducing sugar concentration ranged between 12.36 and 30.12 g/L, and 0.28 and 6.66 g/L, respectively. The satisfactory condition of 9.20 % w/v sulfuric acid at 107.86 0 C was established for reducing sugar and total sugar productions. Furthermore, the non-detoxified rice husk hydrolysate was then used as a carbon source for 10 isolate microbial strains to test PHA production capability. It was found that the highest biomass was observed as 1.01g/l and PHA content as 11.2 %.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"404-410"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kritsana Jatuwong, K. Hyde, S. Chamyuang, K. Matsui, Pattana Kakumyan
The aim of this work was to study the effect of media, temperature and pH on the mycelial growth of Clitopilus chalybescens strain MFLUCC 13–0809 collected from Lampang, Thailand. The antioxidant and antibacterial activities from the crude mycelial extracts were evaluated. The study indicated that the optimal medium, pH and temperature were observed on yeast extract agar (YEA) pH 5 to 7, and 20–29˚C, respectively. For antibacterial activities, crude extracts from mycelium slightly inhibited the growth of Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus), but no activities were observed on Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Antioxidant assay indicated that the crude extract had noticeable scavenging activity on 2,2-diphenyl-1 picrylhydrazyle (DPPH) radical. The results suggest that the crude extract from this mushroom mycelium deem to have a potential for further development on antibacterial and antioxidant applications.
{"title":"Optimization conditions, antibacterial and antioxidant activities of clitopilus chalybescens","authors":"Kritsana Jatuwong, K. Hyde, S. Chamyuang, K. Matsui, Pattana Kakumyan","doi":"10.14456/KKURJ.2016.27","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.27","url":null,"abstract":"The aim of this work was to study the effect of media, temperature and pH on the mycelial growth of Clitopilus chalybescens strain MFLUCC 13–0809 collected from Lampang, Thailand. The antioxidant and antibacterial activities from the crude mycelial extracts were evaluated. The study indicated that the optimal medium, pH and temperature were observed on yeast extract agar (YEA) pH 5 to 7, and 20–29˚C, respectively. For antibacterial activities, crude extracts from mycelium slightly inhibited the growth of Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus), but no activities were observed on Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Antioxidant assay indicated that the crude extract had noticeable scavenging activity on 2,2-diphenyl-1 picrylhydrazyle (DPPH) radical. The results suggest that the crude extract from this mushroom mycelium deem to have a potential for further development on antibacterial and antioxidant applications.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"42-51"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of dietary supplementation of lotus (Nelumbo nucifera Gaertn.) stamen extract (NNSE) on growth performance, feed utilization efficiency and intestinal morphology of Catfish (Clarias gariepinus) were evaluated experimentally in a 7-week feeding trial. Fish with an initial weight of 12.54±0.64 g were completely randomized into 4 groups with 3 replications and reared in circular concrete tanks. Group 1, fish were fed the basal diets and served as the control group. Groups 2-4, fish were fed the diets supplemented with 0.1, 0.5 and 1% of NNSE, respectively. At the end of experiment, it was found that fish fed the diets containing NNSE at different levels significantly increased weight gain, specific growth rate and feed conversion ratio compared to the control (P 0.05). Villi heights and widths, muscularis thickness and goblet cell number in anterior and posterior portion of fish intestines observed under the light microscope were significantly increased in the experimental groups compared to the control group (P<0.05). Additionally, feeding behavior, palatability and feed acceptability did not differ among the groups. The optimal level of NNSE observed was 0.1%. Taken together, these findings reveal that NNSE could be applied in fish feed as a natural feed additive without negative side-effects to improve growth, feed utilization and intestinal morphology in Catfish.
{"title":"Effects of lotus (Nelumbo nucifera Gaertn.) stamen extract on growth performance, feed utilization and intestinal morphology of catfish (Clarias gariepinus)","authors":"P. Munglue","doi":"10.14456/KKURJ.2016.31","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.31","url":null,"abstract":"The effects of dietary supplementation of lotus (Nelumbo nucifera Gaertn.) stamen extract (NNSE) on growth performance, feed utilization efficiency and intestinal morphology of Catfish (Clarias gariepinus) were evaluated experimentally in a 7-week feeding trial. Fish with an initial weight of 12.54±0.64 g were completely randomized into 4 groups with 3 replications and reared in circular concrete tanks. Group 1, fish were fed the basal diets and served as the control group. Groups 2-4, fish were fed the diets supplemented with 0.1, 0.5 and 1% of NNSE, respectively. At the end of experiment, it was found that fish fed the diets containing NNSE at different levels significantly increased weight gain, specific growth rate and feed conversion ratio compared to the control (P 0.05). Villi heights and widths, muscularis thickness and goblet cell number in anterior and posterior portion of fish intestines observed under the light microscope were significantly increased in the experimental groups compared to the control group (P<0.05). Additionally, feeding behavior, palatability and feed acceptability did not differ among the groups. The optimal level of NNSE observed was 0.1%. Taken together, these findings reveal that NNSE could be applied in fish feed as a natural feed additive without negative side-effects to improve growth, feed utilization and intestinal morphology in Catfish.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"7-17"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suthkamol Suttikul, T. Srinorakutara, Ekarat Butivate, Kitti Orasoon
Sugarcane trash, an agricultural by-product, contained 16.21% neutral detergent soluble (NDS), 38.43% hemicellulose, 34.06% cellulose, 5.51% lignin and 5.79% ash on dry solid (DS) basis. After it was pretreated with 2%w/v NaOH followed by 2%w/v H 2 SO 4 in autoclave (121 o C, 15 min), the content of cellulose, hemicellulose and lignin were 73.17%, 7.21% and 3.41%, respectively. The pretreated sugarcane trash was used as substrate for ethanol production in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes, using commercial cellulase and Saccharomyces cerevisiae TISTR 5596 cells. The optimum conditions in a flask scale of SHF process (15% w/v substrate loading hydrolyzed with cellulase 50 FPU/g DS at 50 o C, pH 5.0 and fermentation at 30 o C), and SSF process (20%w/v substrate loading, cellulase 50 FPU/g DS, hydrolysis and fermentation at 35 o C,pH 5.0) were applied to compare the ethanol production in a fermenter. In the SSF process, the highest level of ethanol production was 57.75 g/L, which was 16.26% higher than that of SHF process (48.36 g/L). The SSF process was therefore provided a more efficient method for the utilization of sugarcane trash.
{"title":"Comparison of SHF and SSF processes for ethanol production from alkali-acid pretreated sugarcane trash","authors":"Suthkamol Suttikul, T. Srinorakutara, Ekarat Butivate, Kitti Orasoon","doi":"10.14456/KKURJ.2016.35","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.35","url":null,"abstract":"Sugarcane trash, an agricultural by-product, contained 16.21% neutral detergent soluble (NDS), 38.43% hemicellulose, 34.06% cellulose, 5.51% lignin and 5.79% ash on dry solid (DS) basis. After it was pretreated with 2%w/v NaOH followed by 2%w/v H 2 SO 4 in autoclave (121 o C, 15 min), the content of cellulose, hemicellulose and lignin were 73.17%, 7.21% and 3.41%, respectively. The pretreated sugarcane trash was used as substrate for ethanol production in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes, using commercial cellulase and Saccharomyces cerevisiae TISTR 5596 cells. The optimum conditions in a flask scale of SHF process (15% w/v substrate loading hydrolyzed with cellulase 50 FPU/g DS at 50 o C, pH 5.0 and fermentation at 30 o C), and SSF process (20%w/v substrate loading, cellulase 50 FPU/g DS, hydrolysis and fermentation at 35 o C,pH 5.0) were applied to compare the ethanol production in a fermenter. In the SSF process, the highest level of ethanol production was 57.75 g/L, which was 16.26% higher than that of SHF process (48.36 g/L). The SSF process was therefore provided a more efficient method for the utilization of sugarcane trash.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"229-235"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zecarias Ghebreslasie, D. Premjet, Siripong Permjet
A total of 61 fungi isolate were subjected for screening of ligninolytic enzyme by dye decolorization plate test. The results presented the diversity of 8 orders belonging to 17 families, 30 genera and 25 unidentified cultures. Seven families of Agaricales, three families of Polyporales, two families of Russulales and one family represent for each of the orders, Boletales, Cantharellales, Phallales, Tremellales and Xylariales. The plate test perceived seven categorical groups as: strains decolorizing a single dye implies for secreting only one of the three main enzymes ; a) Phenol red for LiP, b) Azure B for MnP c) RBBR for Lacc; strains decolorizing two of the organic colors relays for secreting only two enzymes as; d) Phenol red and Azure B, e) Phenol red and RBBR, f) Azure B and RBBR; and g) strains which decolorize three organic dyes produce three of the ligninolytic enzymes.
采用染料脱色平板法对61株真菌分离物进行木质素降解酶的筛选。结果表明，该物种有8目，隶属于17科30属，25种未确定的文化。Agaricales有七个科，Polyporales有三个科，Russulales有两个科，Boletales、Cantharellales、Phallales、Tremellales和Xylariales各有一个科。平板试验认为七个分类组:菌株脱色单一染料意味着只分泌三种主要酶中的一种;a)酚红表示LiP, b)青蓝色b表示MnP, c) RBBR表示Lacc;对两种有机颜色进行脱色的菌株只分泌两种酶;d)酚红与天青B, e)酚红与RBBR, f)天青B与RBBR;g)脱色三种有机染料的菌株产生三种木质素分解酶。
{"title":"Screening of fungi producing ligninolytic enzymes","authors":"Zecarias Ghebreslasie, D. Premjet, Siripong Permjet","doi":"10.14456/KKURJ.2016.39","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.39","url":null,"abstract":"A total of 61 fungi isolate were subjected for screening of ligninolytic enzyme by dye decolorization plate test. The results presented the diversity of 8 orders belonging to 17 families, 30 genera and 25 unidentified cultures. Seven families of Agaricales, three families of Polyporales, two families of Russulales and one family represent for each of the orders, Boletales, Cantharellales, Phallales, Tremellales and Xylariales. The plate test perceived seven categorical groups as: strains decolorizing a single dye implies for secreting only one of the three main enzymes ; a) Phenol red for LiP, b) Azure B for MnP c) RBBR for Lacc; strains decolorizing two of the organic colors relays for secreting only two enzymes as; d) Phenol red and Azure B, e) Phenol red and RBBR, f) Azure B and RBBR; and g) strains which decolorize three organic dyes produce three of the ligninolytic enzymes.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"200-209"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of different concentrations of nitric oxide (NO) on postharvest life of lime (Citrus aurantifolia Swingle) was investigated. Lime was immersed with nitric acid at concentration 0, 5, 10 and 20 µg /l for 1 minute then kept at 4 C°. Changes in weight loss, total soluble solids (TSS), fruit firmness, ascorbic acid (vitamin C), and Chlorophyllase activity were evaluated periodically with 7 day intervals during storage. The results showed that lime immersed with 5µg/l SNP was significantly delayed the decrease of weight loss and firmness, also maintained the highest ascorbic acid and delayed the increase of total soluble solids contents during storage. Moreover, lime fruits treated with 20 ug/l SNP increased more slowly Chlorophyllase activity than the other treatments. This application of NO may be a useful method for extending shelf-life and maintaining quality of lime.
{"title":"Effect of nitric oxide on postharvest quality of lime fruit (citrus aurantifolia swingle)","authors":"et.al Amolsiri Nolpradubphan","doi":"10.14456/KKURJ.2016.8","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.8","url":null,"abstract":"The effect of different concentrations of nitric oxide (NO) on postharvest life of lime (Citrus aurantifolia Swingle) was investigated. Lime was immersed with nitric acid at concentration 0, 5, 10 and 20 µg /l for 1 minute then kept at 4 C°. Changes in weight loss, total soluble solids (TSS), fruit firmness, ascorbic acid (vitamin C), and Chlorophyllase activity were evaluated periodically with 7 day intervals during storage. The results showed that lime immersed with 5µg/l SNP was significantly delayed the decrease of weight loss and firmness, also maintained the highest ascorbic acid and delayed the increase of total soluble solids contents during storage. Moreover, lime fruits treated with 20 ug/l SNP increased more slowly Chlorophyllase activity than the other treatments. This application of NO may be a useful method for extending shelf-life and maintaining quality of lime.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"86-96"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suriya Punchai, Jakrapan Wittayapairoj, Krisada Paonariang, Kriangsak Jenwithisuk, O-tur Saeseaw, S. Udomsawaengsup, Chaiyut Thanapiasal
Background: Laparoscopic sleeve gastrectomy (LSG) has been increasingly popular procedure for treatment morbidly obese patients and showing good weight loss and resolution of comorbidity diseases. The objective of this study was to evaluate postoperative outcome in term of operative complication, weight loss and comorbidity improvement after laparoscopic sleeve gastrectomy. Methods: From July 2011 to December 2014 there is 70 patients (45 woman; 64 percent) with an average age of 37.7 years (range; 17-62 years), mean body weight was 126.5 kg (range; 83-250 kg), mean BMI was 45.7 kg/m2 (range; 33-77 kg/m2) who underwent laparoscopic sleeve gastrectomy for treatment morbid obesity. Postoperative time, 1st day postoperative pain, postoperative complication and postoperative follow up data for the body weight change, percent EWL, BMI and improvement of comorbidity at the 6th, 9th,12th, 18th, and 24th months were recorded. Results: The mean operative time was 75 minutes. The mean operative blood loss was 20 ml. The mean 1st day postoperative pain score was 4. No conversion to laparotomy had to be performed. The mean percentages of excessive body weight loss (EWL) were 46.78 percent at 6 months, 59.75 percent at 1 year, 64.53 percent at 18 months, and 62.92 percent at 2 years. The mean BMI were 35.57 kg/m2 at 6 months, 32.69 kg/m2 at 1 year, 29.23 kg/m2 at 18 months, and 29.45 kg/m2 at 2 years. The rate of resolution of OSA was 65.7 percent and improvement of OSA was 31.4 percent. The rate of resolution of hypertension was 68.9 percent and improvement of hypertension was 31.1 percent. The rate of resolution of DM was 70 percent and improvement of DM was 30 percent. The rate of postoperative complication was 4.2 percent. Conclusions: Laparoscopic sleeve gastrectomy is safe and effective procedure for treatment morbidly obese patients. However, weight regained need to evaluated in long term follow up.
{"title":"Outcome of Laparoscopic Sleeve Gastrectomy for Treatment Morbidly Obese Patients in Srinagarind Hospital","authors":"Suriya Punchai, Jakrapan Wittayapairoj, Krisada Paonariang, Kriangsak Jenwithisuk, O-tur Saeseaw, S. Udomsawaengsup, Chaiyut Thanapiasal","doi":"10.14456/KKURJ.2016.10","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.10","url":null,"abstract":"Background: Laparoscopic sleeve gastrectomy (LSG) has been increasingly popular procedure for treatment morbidly obese patients and showing good weight loss and resolution of comorbidity diseases. The objective of this study was to evaluate postoperative outcome in term of operative complication, weight loss and comorbidity improvement after laparoscopic sleeve gastrectomy. Methods: From July 2011 to December 2014 there is 70 patients (45 woman; 64 percent) with an average age of 37.7 years (range; 17-62 years), mean body weight was 126.5 kg (range; 83-250 kg), mean BMI was 45.7 kg/m2 (range; 33-77 kg/m2) who underwent laparoscopic sleeve gastrectomy for treatment morbid obesity. Postoperative time, 1st day postoperative pain, postoperative complication and postoperative follow up data for the body weight change, percent EWL, BMI and improvement of comorbidity at the 6th, 9th,12th, 18th, and 24th months were recorded. Results: The mean operative time was 75 minutes. The mean operative blood loss was 20 ml. The mean 1st day postoperative pain score was 4. No conversion to laparotomy had to be performed. The mean percentages of excessive body weight loss (EWL) were 46.78 percent at 6 months, 59.75 percent at 1 year, 64.53 percent at 18 months, and 62.92 percent at 2 years. The mean BMI were 35.57 kg/m2 at 6 months, 32.69 kg/m2 at 1 year, 29.23 kg/m2 at 18 months, and 29.45 kg/m2 at 2 years. The rate of resolution of OSA was 65.7 percent and improvement of OSA was 31.4 percent. The rate of resolution of hypertension was 68.9 percent and improvement of hypertension was 31.1 percent. The rate of resolution of DM was 70 percent and improvement of DM was 30 percent. The rate of postoperative complication was 4.2 percent. Conclusions: Laparoscopic sleeve gastrectomy is safe and effective procedure for treatment morbidly obese patients. However, weight regained need to evaluated in long term follow up.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"111-120"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alongkorn Sripholtaen, C. Charoenchai, H. Urairong
The use of Microsatellite markers for identifying grapevine varieties. Eighteen primer pairs were selected for identifying amplify 29 grapevine varieties. The result shower that a total of 133 alleles with an average of 7.4 alleles per locus were found to be polymorphic. All alleles size were approximately 112-262 base pair. The cluster analysis using unweighted pair group method with arithmetic mean (UPGMA) based on genetic dissimilarity matrix with 0.9. Twenty nine grapevine varieties were clustered into two major groups. This research indicated that the microsatellite markers could be used to examine and compare the genetic resources among closely samples. The microsatellite maker is useful for discrimination and analysis of genetic diversity of grapevine varieties, and also for grapevine breeding in the future.
{"title":"Application of Microsatellite Markers for Identification of Wine Grape Varieties in Thailand","authors":"Alongkorn Sripholtaen, C. Charoenchai, H. Urairong","doi":"10.14456/KKURJ.2016.9","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.9","url":null,"abstract":"The use of Microsatellite markers for identifying grapevine varieties. Eighteen primer pairs were selected for identifying amplify 29 grapevine varieties. The result shower that a total of 133 alleles with an average of 7.4 alleles per locus were found to be polymorphic. All alleles size were approximately 112-262 base pair. The cluster analysis using unweighted pair group method with arithmetic mean (UPGMA) based on genetic dissimilarity matrix with 0.9. Twenty nine grapevine varieties were clustered into two major groups. This research indicated that the microsatellite markers could be used to examine and compare the genetic resources among closely samples. The microsatellite maker is useful for discrimination and analysis of genetic diversity of grapevine varieties, and also for grapevine breeding in the future.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"97-110"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fruits and vegetables are valuable sources of natural phenolic antioxidants which are known to have beneficial health promoting properties. The main objectives of this study were to compare the antioxidant activities and total phenolic and flavonoid contents of ten ethanolic edible plant extracts (Five species of fruits and vegetables) with varieties of colors.The result showed that purple color of plants, including ethanolic extracts of Brassica oleracea Linn. (40.7±0.56%) and Vigna sesquipedalis (4.52±0.01%) significantly showed the highest TPC and TFC compared with the same species and other species with different colors (p<0.01). Measurement of antioxidant capacity, yellow Capsicum annuum Linn. (58.72±0.52%) and purple Solanum melongena Linn. extract (22.44±1.20%) significantly showed the maximum antioxidant activities by DPPH radical scavenging activity assay and ferric ion reducing antioxidant power (FRAP) assay, respectively (p<0.01). Base on shades of color pigment extracts, green and red pigment had more potential antioxidant activities than other colors. The purple and yellow pigments showed the maximum total phenolic contents by Folin-Ciocalteu reagent. These results concluded that purple color of plants; Brassica oleracea Linn. and Vigna sesquipedalis in this study had the potential to be a suitable source of cheap phenolics and flavonoids as antioxidant compounds.
{"title":"Antioxidant Activities and Total Phenolic Content of Multi-colored Fruits and Vegetables in Thailand","authors":"S. Tinrat","doi":"10.14456/KKURJ.2016.1","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.1","url":null,"abstract":"Fruits and vegetables are valuable sources of natural phenolic antioxidants which are known to have beneficial health promoting properties. The main objectives of this study were to compare the antioxidant activities and total phenolic and flavonoid contents of ten ethanolic edible plant extracts (Five species of fruits and vegetables) with varieties of colors.The result showed that purple color of plants, including ethanolic extracts of Brassica oleracea Linn. (40.7±0.56%) and Vigna sesquipedalis (4.52±0.01%) significantly showed the highest TPC and TFC compared with the same species and other species with different colors (p<0.01). Measurement of antioxidant capacity, yellow Capsicum annuum Linn. (58.72±0.52%) and purple Solanum melongena Linn. extract (22.44±1.20%) significantly showed the maximum antioxidant activities by DPPH radical scavenging activity assay and ferric ion reducing antioxidant power (FRAP) assay, respectively (p<0.01). Base on shades of color pigment extracts, green and red pigment had more potential antioxidant activities than other colors. The purple and yellow pigments showed the maximum total phenolic contents by Folin-Ciocalteu reagent. These results concluded that purple color of plants; Brassica oleracea Linn. and Vigna sesquipedalis in this study had the potential to be a suitable source of cheap phenolics and flavonoids as antioxidant compounds.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This qualitative research was carried out using formal key informant group discussions and household semi-structured interview. The objectives of the research were to evaluate food diversity and security of three ethnic groups at Nam Chat village, XiengKhuang province in northern Lao PDR, where the village includes three ethnic groups; Khmu, Hmong and Lao Loum. The study found that rice was the main staple food crop for three ethnic groups. However, Hmong and Khmu representing approximately 30% of total household in this village produced insufficient rice for household consumption. Food was gathered from various kinds of cultivated crops and domestic livestock, and also included wild plants, wildlife aquatic animals and insects. The three ethnic groups consumed different quantities of food sources from crops or animal species, depending on specific ethnic groups. The three ethnic groups earned household income both from crops and domestic animals, as well as from non-timber forest products and wildlife. Lao Loum received per capita income greater than Hmong and Khmu in the present study.
{"title":"Food diversity of three ethnic groups: a case study from Xieng Khuang province, Northern Lao PDR","authors":"et.al Sonemany Pathumphone","doi":"10.14456/KKURJ.2016.2","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.2","url":null,"abstract":"This qualitative research was carried out using formal key informant group discussions and household semi-structured interview. The objectives of the research were to evaluate food diversity and security of three ethnic groups at Nam Chat village, XiengKhuang province in northern Lao PDR, where the village includes three ethnic groups; Khmu, Hmong and Lao Loum. The study found that rice was the main staple food crop for three ethnic groups. However, Hmong and Khmu representing approximately 30% of total household in this village produced insufficient rice for household consumption. Food was gathered from various kinds of cultivated crops and domestic livestock, and also included wild plants, wildlife aquatic animals and insects. The three ethnic groups consumed different quantities of food sources from crops or animal species, depending on specific ethnic groups. The three ethnic groups earned household income both from crops and domestic animals, as well as from non-timber forest products and wildlife. Lao Loum received per capita income greater than Hmong and Khmu in the present study.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"12-25"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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