Pub Date : 2017-04-26DOI: 10.2208/jscejipm.72.i_801
Pattamaporn Wongwiriya et. al
Studies regarding paratransit in Thailand are mainly concerned with issues such as the role of paratransit focusing on the supply side like service characteristics and service quality. However, the study on Songtaew service in Thailand have not yet well understood. This study is the attempt to focus on the demand side of the Songtaew service in the medium-sized cities in Thailand, including Khon Kaen City by examining the use of Songtaew from the commuters’ point of view by their participation in rating the condition of Songtaew and loyalty to it. Its main objectives are to investigate the workers’ motivations for using or not using Songtaew for traveling to work, and to understand which factors influence workers in Khon Kaen City to use Songtaew. Based on the analysis, the cost and the convenience have a strongly positive impact on the decision to commute by Songtaew and the users want to keep using it in the future.
{"title":"User perception of paratransit in Thailand: case study of journey to work in Khon Kaen city","authors":"Pattamaporn Wongwiriya et. al","doi":"10.2208/jscejipm.72.i_801","DOIUrl":"https://doi.org/10.2208/jscejipm.72.i_801","url":null,"abstract":"Studies regarding paratransit in Thailand are mainly concerned with issues such as the role of paratransit focusing on the supply side like service characteristics and service quality. However, the study on Songtaew service in Thailand have not yet well understood. This study is the attempt to focus on the demand side of the Songtaew service in the medium-sized cities in Thailand, including Khon Kaen City by examining the use of Songtaew from the commuters’ point of view by their participation in rating the condition of Songtaew and loyalty to it. Its main objectives are to investigate the workers’ motivations for using or not using Songtaew for traveling to work, and to understand which factors influence workers in Khon Kaen City to use Songtaew. Based on the analysis, the cost and the convenience have a strongly positive impact on the decision to commute by Songtaew and the users want to keep using it in the future.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76301350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water hyacinth contains cellulose and hemicellulose which can be used as a substrate for bio-hydrogen production. Clostridium diolis C32-KKU, a cellulolytic bacterium, was employed to directly ferment water hyacinth to bio-hydrogen. The objective of this study was to optimize the direct bio-hydrogen production from water hyacinth by C. diolis C32-KKU. Two operation modes for bio-hydrogen production i.e. static and shaking modes were investigated. The results showed that the shaking mode was more effective than the static mode for hydrogen production. The shaking mode was then used to optimize bio-hydrogen production by variation of initial pH (4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0) and initial water hyacinth concentration (5, 10, 15, 20, 25 and 30 g-dry weight (dw)/L). The maximum hydrogen production of 19 mL/L was obtained at the initial pH of 5.5 and water hyacinth concentration of 10 g-dw/L. The cellulase activity of 0.0081 unit/mL was obtained under the optimal condition. The results of this study showed that direct bio-hydrogen production from lignocellulosic materials could be feasible.
{"title":"Optimization of conditions for direct bio-hydrogen production from water hyacinth by clostridium diolis C32-KKU","authors":"P. Muanruksa, Nadda Khongsay, K. Fiala","doi":"10.14456/KKURJ.2016.55","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.55","url":null,"abstract":"Water hyacinth contains cellulose and hemicellulose which can be used as a substrate for bio-hydrogen production. Clostridium diolis C32-KKU, a cellulolytic bacterium, was employed to directly ferment water hyacinth to bio-hydrogen. The objective of this study was to optimize the direct bio-hydrogen production from water hyacinth by C. diolis C32-KKU. Two operation modes for bio-hydrogen production i.e. static and shaking modes were investigated. The results showed that the shaking mode was more effective than the static mode for hydrogen production. The shaking mode was then used to optimize bio-hydrogen production by variation of initial pH (4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0) and initial water hyacinth concentration (5, 10, 15, 20, 25 and 30 g-dry weight (dw)/L). The maximum hydrogen production of 19 mL/L was obtained at the initial pH of 5.5 and water hyacinth concentration of 10 g-dw/L. The cellulase activity of 0.0081 unit/mL was obtained under the optimal condition. The results of this study showed that direct bio-hydrogen production from lignocellulosic materials could be feasible.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Teeka, Manoch Posung, Sutida Tuntikumthon, Jariya Ubonrat, Jintana Kusonsong, Rachadaphorn Pikulsiri, Supattra Kurakhamsang, Tsuyoshi Ima
Polyhydroxyalkanoates (PHA) production by Novosphingobium sp. THA_AIK7 was investigated in Mineral salt medium (MSM) supplemented with 2% (v/v) of crude glycerol as a carbon source. The polymer were extracted by sodium hypochlorite and chloroform. Both of extracted films was white, opaque and has a smooth surface. PHA film casting from sodium hypochlorite extraction had bigger pore size than chloroform casted film. The contact angle obtained from polystyrene tissue culture plate (TCP), sodium hypochlorite and chloroform casted film were 78, 81 and 80 degree, respectively. Decomposition temperature (Td), melting temperature (Tm) and crystallization temperature (Tc) were examined against PHB standard. Td of 285, 299 and 298 ° C were acquired from PHB, sodium hypochlorite and chloroform casted film, respectively. Tm value of 170, 167 and 178 ° C and Tc value of 66, 54 and 92 ° C were acquired from sodium hypochlorite, chloroform and PHB, respectively. Biocompatibility study of PHA film was tested with Vero cell. Vero cells grown on tissue culture plate (TCP) presented typical fibroblast morphology. However, Vero cells were unable to adhere on sodium hypochlorite casted film and consequently cell lysis was observed. Vero cells could attach on chloroform casted film but could not develop normal fibroblast appearance and was incapable of proliferating on casted film. The results shown here indicated that the extraction methods were still not proper for biocompatible test. The finding of a suitable method for biomaterial preparation will be done in the next phase.
{"title":"Influence of Solvent Extract on Properties of Biomaterial PHA Derived from Novosphingobium sp. THA_AIK7","authors":"J. Teeka, Manoch Posung, Sutida Tuntikumthon, Jariya Ubonrat, Jintana Kusonsong, Rachadaphorn Pikulsiri, Supattra Kurakhamsang, Tsuyoshi Ima","doi":"10.14456/KKURJ.2016.49","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.49","url":null,"abstract":"Polyhydroxyalkanoates (PHA) production by Novosphingobium sp. THA_AIK7 was investigated in Mineral salt medium (MSM) supplemented with 2% (v/v) of crude glycerol as a carbon source. The polymer were extracted by sodium hypochlorite and chloroform. Both of extracted films was white, opaque and has a smooth surface. PHA film casting from sodium hypochlorite extraction had bigger pore size than chloroform casted film. The contact angle obtained from polystyrene tissue culture plate (TCP), sodium hypochlorite and chloroform casted film were 78, 81 and 80 degree, respectively. Decomposition temperature (Td), melting temperature (Tm) and crystallization temperature (Tc) were examined against PHB standard. Td of 285, 299 and 298 ° C were acquired from PHB, sodium hypochlorite and chloroform casted film, respectively. Tm value of 170, 167 and 178 ° C and Tc value of 66, 54 and 92 ° C were acquired from sodium hypochlorite, chloroform and PHB, respectively. Biocompatibility study of PHA film was tested with Vero cell. Vero cells grown on tissue culture plate (TCP) presented typical fibroblast morphology. However, Vero cells were unable to adhere on sodium hypochlorite casted film and consequently cell lysis was observed. Vero cells could attach on chloroform casted film but could not develop normal fibroblast appearance and was incapable of proliferating on casted film. The results shown here indicated that the extraction methods were still not proper for biocompatible test. The finding of a suitable method for biomaterial preparation will be done in the next phase.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66677054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Phunpruch, Amornrat Puangplub, A. Incharoensakdi
Hydrogen produced by cyanobacteria and green algae is a very interesting energy carrier because it is produced by a photosynthetic pathway using sunlight as an energy source. In this study, 59 cyanobacterial and green algal strains were isolated from soil and water sources of rice paddle field in Thailand. Out of them, 9 cyanobacterial isolates and 9 green algal isolates were purified. Among them, unicellular cyanobacterial isolate AngS1 showed the highest H 2 production rate. Its highest H 2 production rate of 389.630±72.084 nmolH 2 mg chl -1 h -1 was found in cells grown in BG11 for 1 week followed by incubating cells in BG11 0 for 24 hours and adaptation under dark anaerobic condition for 2 hours. The optimal concentrations of glucose, MgSO 4 .7H 2 O and Fe 3+ for H 2 production rate were 0.189 mmolC L -1 , 3 mM, and 20 μM, respectively. The highest H 2 accumulation of 4,174.364±278.324 nmolH 2 mg chl -1 was obtained when incubating cells in the optimal medium for 11 days.
{"title":"Biohydrogen production by microalgae isolated from the rice paddle field in Thailand","authors":"S. Phunpruch, Amornrat Puangplub, A. Incharoensakdi","doi":"10.14456/KKURJ.2016.33","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.33","url":null,"abstract":"Hydrogen produced by cyanobacteria and green algae is a very interesting energy carrier because it is produced by a photosynthetic pathway using sunlight as an energy source. In this study, 59 cyanobacterial and green algal strains were isolated from soil and water sources of rice paddle field in Thailand. Out of them, 9 cyanobacterial isolates and 9 green algal isolates were purified. Among them, unicellular cyanobacterial isolate AngS1 showed the highest H 2 production rate. Its highest H 2 production rate of 389.630±72.084 nmolH 2 mg chl -1 h -1 was found in cells grown in BG11 for 1 week followed by incubating cells in BG11 0 for 24 hours and adaptation under dark anaerobic condition for 2 hours. The optimal concentrations of glucose, MgSO 4 .7H 2 O and Fe 3+ for H 2 production rate were 0.189 mmolC L -1 , 3 mM, and 20 μM, respectively. The highest H 2 accumulation of 4,174.364±278.324 nmolH 2 mg chl -1 was obtained when incubating cells in the optimal medium for 11 days.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Waesarat Soontornchaiboon, O. Chunhachart, Ratchapol Pawongrat
The ethanol production from alkaline pretreated pineapple waste (core and peel) by using co-culture process of S. cerevisiae TISTR 5339 and C. shehatae KCCM 11422 was evaluated. The potential of ultrasound-assisted alkaline pretreatment was applied to enhance total reducing sugar concentration. The highest yield of total reducing sugar was obtained at 21.84 g/g dried sample after pretreatment by 2% NaOH with ultrasonic-assistance for 60 min subsequently by enzymatic hydrolysis. The compositions of pretreated pineapple waste were 40.52±0.99% cellulose, 24.03±1.52% hemicellulose and 2.27±0.23% lignin. However, the dry matter loss reached to 44.67±3.21% after pretreatment. The structural changes of pretreated pineapple waste were observed by scanning electron microscopy (SEM). The maximum ethanol concentration and ethanol yield after 24 h fermentation were 9.73±0.15g/L and 0.45±0.01 g/g, respectively.
{"title":"Ethanol Production from Pineapple Waste by Co-culture of Saccharomyces cerevisiae TISTR 5339 and Candida shehatae KCCM 11422","authors":"Waesarat Soontornchaiboon, O. Chunhachart, Ratchapol Pawongrat","doi":"10.14456/KKURJ.2016.48","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.48","url":null,"abstract":"The ethanol production from alkaline pretreated pineapple waste (core and peel) by using co-culture process of S. cerevisiae TISTR 5339 and C. shehatae KCCM 11422 was evaluated. The potential of ultrasound-assisted alkaline pretreatment was applied to enhance total reducing sugar concentration. The highest yield of total reducing sugar was obtained at 21.84 g/g dried sample after pretreatment by 2% NaOH with ultrasonic-assistance for 60 min subsequently by enzymatic hydrolysis. The compositions of pretreated pineapple waste were 40.52±0.99% cellulose, 24.03±1.52% hemicellulose and 2.27±0.23% lignin. However, the dry matter loss reached to 44.67±3.21% after pretreatment. The structural changes of pretreated pineapple waste were observed by scanning electron microscopy (SEM). The maximum ethanol concentration and ethanol yield after 24 h fermentation were 9.73±0.15g/L and 0.45±0.01 g/g, respectively.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66677034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zymomonas mobilis biofilm have been proposed to enhance the bioethanol production from agricultural derived materials. Z. mobilis biofilm reactor has been prospected to be used for a large scale bioethanol production. The cost effective carrier for Z. mobilis biofilm reactor was searched. This study investigated the biofilm forming abilities of Z. mobilis strain TISTR 551 and ZM4 on biotic (loofah and corn silk) or abiotic carriers (flatted sheet polyvinyl chloride, PVC). Biofilm formation was visualized for 3 consecutive days under the bright-field microscope. Only Z. mobilis TISTR551 represented the biofilm forming ability on corn silk under the microscopic observation, while no biofilm formation on loofah and PVC was observed. The mature biofilm was developed on day 3. The biofilm formation was also quantitatively analyzed based on the weight differentiation of the carrier and the carrier with the bacterial attachment. The net biomass weight of TISTR 551 and ZM4 on corn silk carrier was 0.6 ± 0.1 g and 0.33 ± 0.1g respectively. Therefore, corn silk illustrates its potential to be used as a cost effective biocarrier for Z. mobilis biofilm.
{"title":"Zymomonas mobilis biofilm formations on different types of carriers","authors":"Tatsaporn Todhanakasem, R. Tiwari","doi":"10.14456/KKURJ.2016.14","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.14","url":null,"abstract":"Zymomonas mobilis biofilm have been proposed to enhance the bioethanol production from agricultural derived materials. Z. mobilis biofilm reactor has been prospected to be used for a large scale bioethanol production. The cost effective carrier for Z. mobilis biofilm reactor was searched. This study investigated the biofilm forming abilities of Z. mobilis strain TISTR 551 and ZM4 on biotic (loofah and corn silk) or abiotic carriers (flatted sheet polyvinyl chloride, PVC). Biofilm formation was visualized for 3 consecutive days under the bright-field microscope. Only Z. mobilis TISTR551 represented the biofilm forming ability on corn silk under the microscopic observation, while no biofilm formation on loofah and PVC was observed. The mature biofilm was developed on day 3. The biofilm formation was also quantitatively analyzed based on the weight differentiation of the carrier and the carrier with the bacterial attachment. The net biomass weight of TISTR 551 and ZM4 on corn silk carrier was 0.6 ± 0.1 g and 0.33 ± 0.1g respectively. Therefore, corn silk illustrates its potential to be used as a cost effective biocarrier for Z. mobilis biofilm.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Sirimuangmoon, Soh-Min Lee, J. Guinard, A. M. Miller
Many diet-related chronic diseases are increasingly affecting to the health of the people all around the world because of their unhealthy dietary choices and habits. Nutritionists have suggested the consumers to change their food-intake patterns from the foods that contain high sugar, fat, salt and meat content to a more plant-based diet. Mushroom is one of the plant-based alternatives that can be used as a healthy substitute for meat and a mitigating agent for sodium reduction. Six beef taco blend recipes with different amount of meat, mushroom, and salt were evaluated by a group of 147 consumers, currently living in Northern California. Result from correlation analysis clearly revealed that the most important drivers of liking were flavor and texture. Appearance was not significantly correlated with overall liking. In general, consumers liked the samples without any salt reduction more than the samples with reduced salt. No meat substitute (100% beef), 50% and 80% meat substitute samples were all samples that received the highest degree of liking. The analysis of saltiness on Just-about-right (JAR) scale showed that 50% and 80% meat substitute samples were reported to have 60% just-right level of saltiness whereas 100% beef sample got only 48%. Four clusters were obtained from cluster analysis based on overall degree of liking. Consumers in the first cluster like 100% beef and 80% meat substitute samples. The second cluster was the largest cluster. They preferred to eat meat/mushroom blends. The third cluster was a group of plant based-diet consumers. They disliked 100% beef sample but liked all mushroom-based blends. The last cluster was a group of consumers who liked 100% beef sample most. Among all four clusters, this group of consumers tended to give lower hedonic scores all over samples than the others groups. These findings could be useful to devise culinary strategies and sensory insights to improve the flavor and consumer appeal of health-promoting plant-based foods without negatively affecting nutrition quality.
{"title":"A Study of Using Mushrooms as a Plant-based Alternative for a Popular Meat-based Dish","authors":"C. Sirimuangmoon, Soh-Min Lee, J. Guinard, A. M. Miller","doi":"10.14456/KKURJ.2016.15","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.15","url":null,"abstract":"Many diet-related chronic diseases are increasingly affecting to the health of the people all around the world because of their unhealthy dietary choices and habits. Nutritionists have suggested the consumers to change their food-intake patterns from the foods that contain high sugar, fat, salt and meat content to a more plant-based diet. Mushroom is one of the plant-based alternatives that can be used as a healthy substitute for meat and a mitigating agent for sodium reduction. Six beef taco blend recipes with different amount of meat, mushroom, and salt were evaluated by a group of 147 consumers, currently living in Northern California. Result from correlation analysis clearly revealed that the most important drivers of liking were flavor and texture. Appearance was not significantly correlated with overall liking. In general, consumers liked the samples without any salt reduction more than the samples with reduced salt. No meat substitute (100% beef), 50% and 80% meat substitute samples were all samples that received the highest degree of liking. The analysis of saltiness on Just-about-right (JAR) scale showed that 50% and 80% meat substitute samples were reported to have 60% just-right level of saltiness whereas 100% beef sample got only 48%. Four clusters were obtained from cluster analysis based on overall degree of liking. Consumers in the first cluster like 100% beef and 80% meat substitute samples. The second cluster was the largest cluster. They preferred to eat meat/mushroom blends. The third cluster was a group of plant based-diet consumers. They disliked 100% beef sample but liked all mushroom-based blends. The last cluster was a group of consumers who liked 100% beef sample most. Among all four clusters, this group of consumers tended to give lower hedonic scores all over samples than the others groups. These findings could be useful to devise culinary strategies and sensory insights to improve the flavor and consumer appeal of health-promoting plant-based foods without negatively affecting nutrition quality.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The infant intestinal tract is essentially sterile part and experiences a period of steady colonization over the following weeks and months as it is exposed to microorganisms from the environment. It is generally accepted that these microorganisms have a major impact on the overall development and function of the gastrointestinal mucosa and immune system. This research aims at prebiotic extraction from wild yam which is a plant in the local area. The study found that prebiotic extracted from wild yam soaked for 24 hours and 3 days gave the prebiotic concentration of 1,437 μg/ml and 1,141 μg/ml, respectively. The optimum conditions for prebiotic extraction consisted of the extraction ratio, temperature and soaking time of 1:30 (w/v), 85°C and 30 minutes, respectively. The growth of Lactobacillus casei subsp. rhamnosus in fermented milk supplemented with wild yam extract was investigated. Result showed that the fermented milk with prebiotic extract (3 days soaking wild yam) was able to promote growth of L. casei subsp. rhamnosus.
{"title":"The potential of prebiotic production from wild yam as lactobacillus casei growth promoter","authors":"Phanrawi Muadsri","doi":"10.14456/KKURJ.2016.20","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.20","url":null,"abstract":"The infant intestinal tract is essentially sterile part and experiences a period of steady colonization over the following weeks and months as it is exposed to microorganisms from the environment. It is generally accepted that these microorganisms have a major impact on the overall development and function of the gastrointestinal mucosa and immune system. This research aims at prebiotic extraction from wild yam which is a plant in the local area. The study found that prebiotic extracted from wild yam soaked for 24 hours and 3 days gave the prebiotic concentration of 1,437 μg/ml and 1,141 μg/ml, respectively. The optimum conditions for prebiotic extraction consisted of the extraction ratio, temperature and soaking time of 1:30 (w/v), 85°C and 30 minutes, respectively. The growth of Lactobacillus casei subsp. rhamnosus in fermented milk supplemented with wild yam extract was investigated. Result showed that the fermented milk with prebiotic extract (3 days soaking wild yam) was able to promote growth of L. casei subsp. rhamnosus.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antioxidants, substances that prevent damage from free radicals ,have originally been discovered in various natural sources including fruit seeds. In longan seeds, a potential antioxidative peptide with amino acid sequence as ISYVVPVYIAEITPKT - FRGGF, was found. In this research, to overcome protein hydrolysate preparation problems, genetic engineering was used to produce arecombinant version of this antioxidative peptide in Escherichia coli. For the easier genetic manipulation, the DNA fragment for encoding the target peptide was designed by containing 4 copies of the interested peptide and each copy was linked by a codon of Aspartic acid. After IPTG induction, the recombinant peptide was successfully expressed and purified. The recombinant peptide was verified by Endoproteinase AspN digestion and MALDI - TOF-MS. The antioxidant activity of this recombinant peptide will be further studied
{"title":"Construction of Escherchia coli strain for producing recombinant antioxidative peptide from longan seeds","authors":"Thanaporn Wichai, R. Boonsombat","doi":"10.14456/KKURJ.2016.37","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.37","url":null,"abstract":"Antioxidants, substances that prevent damage from free radicals ,have originally been discovered in various natural sources including fruit seeds. In longan seeds, a potential antioxidative peptide with amino acid sequence as ISYVVPVYIAEITPKT - FRGGF, was found. In this research, to overcome protein hydrolysate preparation problems, genetic engineering was used to produce arecombinant version of this antioxidative peptide in Escherichia coli. For the easier genetic manipulation, the DNA fragment for encoding the target peptide was designed by containing 4 copies of the interested peptide and each copy was linked by a codon of Aspartic acid. After IPTG induction, the recombinant peptide was successfully expressed and purified. The recombinant peptide was verified by Endoproteinase AspN digestion and MALDI - TOF-MS. The antioxidant activity of this recombinant peptide will be further studied","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A fungal strain, Rhizopus sp. C018, is a promising filamentous fungus that produces L-(+)-lactic acid. In this study the wild strain of C018 was used for lactic acid production by solid state fermentation on cassava peel incubated at 30oC. The results showed that the strain could produce 32.4 mg/g of lactic acid at day three. Then, the wild strain of C018 was subjected to mutation by UV radiation and the mutant named UV 333 expressed the highest lactic acid production as 57.6 mg/g analyzed by Reflectometer RQflex® 10 Merck Germany at day three. For further studies, mutation by chemicals will be tested to obtain the maximum amount of lactic acid production.
{"title":"The use of Rhizopus sp. mutant for lactic acid production by solid state fermentation","authors":"Saffra Hayihama, Wilailak Suwazono","doi":"10.14456/KKURJ.2016.25","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.25","url":null,"abstract":"A fungal strain, Rhizopus sp. C018, is a promising filamentous fungus that produces L-(+)-lactic acid. In this study the wild strain of C018 was used for lactic acid production by solid state fermentation on cassava peel incubated at 30oC. The results showed that the strain could produce 32.4 mg/g of lactic acid at day three. Then, the wild strain of C018 was subjected to mutation by UV radiation and the mutant named UV 333 expressed the highest lactic acid production as 57.6 mg/g analyzed by Reflectometer RQflex® 10 Merck Germany at day three. For further studies, mutation by chemicals will be tested to obtain the maximum amount of lactic acid production.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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