Fungal diversity in the seawater and sediment in the mangrove forest at Andaman Coastal Research Station for Development, Ranong Province was studied. Seawater and sediment were sampled in 5 sites during the rainy and dry season. The fungi were cultivated in Potato Dextrose Agar (PDA) supplemented with Streptomycin and Chloramphenical and incubated at room temperature for 7 days. Eighty nine and 99 fungal isolates were found in the sediment and seawater samples, respectively. Fungal identification was done using their molecular study. Forty-five fungal species could be identified using molecular study and morphological features including: Acremonium furcatum, A. nepalense, Aspergillus aculeatus, A. flavus, A. fumigatus, A. niger, A. nomius, A. nutans, A. oryzae, A. tubingensis, Aspergillus sp., Ceratocystis paradoxa, Cladosporium cladosporioides, C. oxysporum, Colletotrichum gloeosporioides, Diaporthe helianthi, Didymellaceae sp., Dothideomycetes sp., Eupenicillium shearii, Fusarium equiseti, F. moniliformis, F. nelsonii, F. oxysporum, F. proliferatum, F. solani, F. subglutinans, Fusarium sp., Ganoderma cupreum, Gliocephalotrichum simplex, Gongronella butleri, Hypocrea jecorina, Lasiodiplodia theobromae, Neosartorya fischeri, Penicillium citrinum, P. funiculosum, P. griseofulvum, P. janthinellum, P. lilacinum, P. oxalicum, P. rademirici, P. simplicissimum, P. stecki, P. vasconiae, Penicillium sp., Pestalotiopsis mangiferae, Pleurotus pulmonarius, Rigidoporus vinctus, Sordaria sp., Talaromyces assiutensis, Trichaptum laricinum, Trichoderma asperellum, Trichoderma sp. and Xylaria apoda. The common fungal species found in this study were Penicillium, Aspergillus and Fusarium.
{"title":"Biodiversity of fungi in seawater and sediment from mangrove forest at Andaman coastal research station for development, Ranong province","authors":"et.al Umaporn Phonrod","doi":"10.14456/KKURJ.2016.7","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.7","url":null,"abstract":"Fungal diversity in the seawater and sediment in the mangrove forest at Andaman Coastal Research Station for Development, Ranong Province was studied. Seawater and sediment were sampled in 5 sites during the rainy and dry season. The fungi were cultivated in Potato Dextrose Agar (PDA) supplemented with Streptomycin and Chloramphenical and incubated at room temperature for 7 days. Eighty nine and 99 fungal isolates were found in the sediment and seawater samples, respectively. Fungal identification was done using their molecular study. Forty-five fungal species could be identified using molecular study and morphological features including: Acremonium furcatum, A. nepalense, Aspergillus aculeatus, A. flavus, A. fumigatus, A. niger, A. nomius, A. nutans, A. oryzae, A. tubingensis, Aspergillus sp., Ceratocystis paradoxa, Cladosporium cladosporioides, C. oxysporum, Colletotrichum gloeosporioides, Diaporthe helianthi, Didymellaceae sp., Dothideomycetes sp., Eupenicillium shearii, Fusarium equiseti, F. moniliformis, F. nelsonii, F. oxysporum, F. proliferatum, F. solani, F. subglutinans, Fusarium sp., Ganoderma cupreum, Gliocephalotrichum simplex, Gongronella butleri, Hypocrea jecorina, Lasiodiplodia theobromae, Neosartorya fischeri, Penicillium citrinum, P. funiculosum, P. griseofulvum, P. janthinellum, P. lilacinum, P. oxalicum, P. rademirici, P. simplicissimum, P. stecki, P. vasconiae, Penicillium sp., Pestalotiopsis mangiferae, Pleurotus pulmonarius, Rigidoporus vinctus, Sordaria sp., Talaromyces assiutensis, Trichaptum laricinum, Trichoderma asperellum, Trichoderma sp. and Xylaria apoda. The common fungal species found in this study were Penicillium, Aspergillus and Fusarium.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"77-85"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TiO2 powder was prepared by microwave-assisted sol-gel method. The powder was refluxed at 180 W for 1, 2 and 3 h and dried at 180 W for 1 h by a conventional microwave oven. Several analytical techniques including X-ray diffraction (XRD), scanning electron microscopy (SEM) and surface area measurement (BET) were employed to characterize the synthesized powder. Photocatalytic activity of the powder was examined via the degradation of methylene blue (MB) solution under UV irradiation for a certain time. The efficiency of antibacterial activity was evaluated by the inactivation of Staphylococcus aureus (S. aureus). The results showed that only anatase TiO2 was observed and the as-prepared powder exhibited the agglomeration of spherical nanoparticles with crystallite sizes of 20.7, 13.8 and 9.3 nm when the refluxed time was 1, 2 and 3 h, respectively. The highest efficiency for the photocatalytic and antibacterial activities was 66.68 and 91.67%, respectively, belonging to those powders using the reflux time of 3 h.
{"title":"Study of the photocatalytic and antibacterial activity of tio2 powder synthesized by microwave-assisted sol-gel method","authors":"W. Sangchay","doi":"10.14456/KKURJ.2016.6","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.6","url":null,"abstract":"TiO2 powder was prepared by microwave-assisted sol-gel method. The powder was refluxed at 180 W for 1, 2 and 3 h and dried at 180 W for 1 h by a conventional microwave oven. Several analytical techniques including X-ray diffraction (XRD), scanning electron microscopy (SEM) and surface area measurement (BET) were employed to characterize the synthesized powder. Photocatalytic activity of the powder was examined via the degradation of methylene blue (MB) solution under UV irradiation for a certain time. The efficiency of antibacterial activity was evaluated by the inactivation of Staphylococcus aureus (S. aureus). The results showed that only anatase TiO2 was observed and the as-prepared powder exhibited the agglomeration of spherical nanoparticles with crystallite sizes of 20.7, 13.8 and 9.3 nm when the refluxed time was 1, 2 and 3 h, respectively. The highest efficiency for the photocatalytic and antibacterial activities was 66.68 and 91.67%, respectively, belonging to those powders using the reflux time of 3 h.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"67-76"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Applications of a single model may not be able to capture different data patterns well enough, especially in the tourist forecast problem which is often complex in nature. An autoregressive integrated moving average (ARIMA) is a famous linear model while an artificial neural network (ANN) is a promising alternative to a traditional linear method. The ARIMA model may not be adequate for nonlinear problems while ANN can well reveal the correlation of nonlinear patterns. However, overfitting due to a learning process is the main disadvantage of ANN as well as being trapped in a local optimum for parameters optimization. To improve the forecast performance of both ARIMA and ANN for high accuracy, the two hybridization models, i.e. hybrid ARIMA-RBFNN model and hybrid RBFNN-ARIMA model are employed to examine the Chiangmai’s tourist time series data. Statistics test and parameter designed experiments were used to optimize these models and the sum-square of error (SSE) was used to indicate their performances. In this case study, the hybrid RBFNN-ARIMA model has proved that the RBFNN can priori capture the non-stationary non-linear component while the fully linearly stationary residuals were accurately predicted by ARIMA. The experimental results demonstrated that the hybrid RBFNN-ARIMA model outperformed 42% by averaging over the hybrid ARIMA-RBFNN model, an improvement of hybrid ARIMA-RBFNN model, RBFNN model, and ARIMA model.
{"title":"A Hybrid ARIMA and RBF Neural Network Model for Tourist Quantity Forecasting : A Case Study for Chiangmai Province","authors":"et.al Rati Wongsathan","doi":"10.14456/KKURJ.2016.4","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.4","url":null,"abstract":"Applications of a single model may not be able to capture different data patterns well enough, especially in the tourist forecast problem which is often complex in nature. An autoregressive integrated moving average (ARIMA) is a famous linear model while an artificial neural network (ANN) is a promising alternative to a traditional linear method. The ARIMA model may not be adequate for nonlinear problems while ANN can well reveal the correlation of nonlinear patterns. However, overfitting due to a learning process is the main disadvantage of ANN as well as being trapped in a local optimum for parameters optimization. To improve the forecast performance of both ARIMA and ANN for high accuracy, the two hybridization models, i.e. hybrid ARIMA-RBFNN model and hybrid RBFNN-ARIMA model are employed to examine the Chiangmai’s tourist time series data. Statistics test and parameter designed experiments were used to optimize these models and the sum-square of error (SSE) was used to indicate their performances. In this case study, the hybrid RBFNN-ARIMA model has proved that the RBFNN can priori capture the non-stationary non-linear component while the fully linearly stationary residuals were accurately predicted by ARIMA. The experimental results demonstrated that the hybrid RBFNN-ARIMA model outperformed 42% by averaging over the hybrid ARIMA-RBFNN model, an improvement of hybrid ARIMA-RBFNN model, RBFNN model, and ARIMA model.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"37-54"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of decomposition, nitrogen release and soil fertility of upland rice-legume residues incorporation before sugarcane planting was investigated. The experimental design was randomized complete block design (RCBD) with 4 replications. The following residue and control treatments were implemented: i.e. i) control; no residues incorporation, ii) weed, iii) soybean (Glycine max), iv) sunn hemp (Crotalaria juncea) and v) upland rice. Soil samples and litter bags were collected at 1, 2, 4, 8, 12, 16, 24 and 32 weeks after incorporation. Soil mineral N was analyzed by Flow Injection Analyzer (FIA) and remaining dry weights of litter bag were recorded. Soil microbial biomass was measured by chloroform fumigation extraction method. The results revealed that weed residues treatment had the highest of remaining dry weight and % N remaining in litter bag in most sampling dates. Sunn hemp residues treatment had the highest soil mineral N and was significantly different from the other treatments (p < 0.01) at 4, 8, 12 and 16 weeks after residues incorporation. At final sampling date, the upland rice residues treatment gave the highest microbial biomass N and was significantly different from the other treatments (p < 0.01) and provided the highest relative microbial biomass N but not significantly different from soybean residues treatment. Moreover, soil organic matter could be improved by all residue treatments. However, sunn hemp may be a potential crop for soil N release but upland rice could support rice consumption demand. Thus, the effect of legume-upland rice residue incorporation on sugarcane yield should be further investigated under field conditions.
{"title":"Decomposition, Nitrogen Release and Soil Fertility of Plant Residues Incorporation from Different Pre- Sugarcane Planting Management","authors":"et.al Sucharat Butphu","doi":"10.14456/KKURJ.2016.3","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.3","url":null,"abstract":"The effect of decomposition, nitrogen release and soil fertility of upland rice-legume residues incorporation before sugarcane planting was investigated. The experimental design was randomized complete block design (RCBD) with 4 replications. The following residue and control treatments were implemented: i.e. i) control; no residues incorporation, ii) weed, iii) soybean (Glycine max), iv) sunn hemp (Crotalaria juncea) and v) upland rice. Soil samples and litter bags were collected at 1, 2, 4, 8, 12, 16, 24 and 32 weeks after incorporation. Soil mineral N was analyzed by Flow Injection Analyzer (FIA) and remaining dry weights of litter bag were recorded. Soil microbial biomass was measured by chloroform fumigation extraction method. The results revealed that weed residues treatment had the highest of remaining dry weight and % N remaining in litter bag in most sampling dates. Sunn hemp residues treatment had the highest soil mineral N and was significantly different from the other treatments (p < 0.01) at 4, 8, 12 and 16 weeks after residues incorporation. At final sampling date, the upland rice residues treatment gave the highest microbial biomass N and was significantly different from the other treatments (p < 0.01) and provided the highest relative microbial biomass N but not significantly different from soybean residues treatment. Moreover, soil organic matter could be improved by all residue treatments. However, sunn hemp may be a potential crop for soil N release but upland rice could support rice consumption demand. Thus, the effect of legume-upland rice residue incorporation on sugarcane yield should be further investigated under field conditions.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"62 2 1","pages":"26-36"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The endophytic fungi that colonizes internal plant tissues without causing apparent harm to their host were isolated from healthy leaf, root and stem of Hottuynia cordata Thunb. Twelve fungal isolates found were identified by their morphological characteristic and using molecular technique. The result showed that ten isolates belonging to Ascomycota were presumed to be in genus Colletotrichum, Lasiodiplodia and Fusarium in eight isolates and two isolates were identified to class Dothideomycetes and Sordariomycetes. Two unidentified isolates (R14 and R15), confined only to root segments were isolated, these members may be from the group of Mycelia-Sterilia. The evaluation of antagonistic activity of twelve fungal endophytes isolated from healthy Houttuynia cordata Thumb against Trichoderma harzianum and five plant pathogenic fungi (Fusarium oxysporum, Sclerotium rolfsii, Rhizoctonia sp., Alternaria brassicicola and Phytophthora palmivora) by dual culture technique were performed. The in vitro antagonistic activities as two types of activities in this study; mycoparasitism and competition were found. Endophyte isolate B06, B12, B09 and R15 gave the highest percent inhibition of radial growth against to the plant pathogenic fungi (F. oxysporum, S. rolfsii, A. brassicicola) and T. harzianum within 24.22%, 29.07%, 13.54 and 24.15%, respectively. Endophyte isolate R14 showed the highest antagonistic activity to Rhizoctonia sp. and P. palmivora with 39.33% and 33.34% respectively. For the siderophore production, the seven active endophyte isolates showed orange halo formed around the colonies more than 30 mm on CAS agar. Three endophyte isolates efficiently produced IAA more than 24 µg/ml. The active endophyte isolates B06 identified as Lasiodiplodia pseudotheobromae showed the highest ability to produce IAA (37.034 µg/ml) and also showed the highest orange halo formed around the colonies which determined to siderophore production.
{"title":"Studies on antagonistic effect against plant pathogenic fungi from Endophytic fungi isolated from Hottuynia Cordata Thunb and screening for Siderophore and indole-3-acetic acid production","authors":"et.al Yaovapa Aramsirirujiwet","doi":"10.14456/KKURJ.2016.5","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.5","url":null,"abstract":"The endophytic fungi that colonizes internal plant tissues without causing apparent harm to their host were isolated from healthy leaf, root and stem of Hottuynia cordata Thunb. Twelve fungal isolates found were identified by their morphological characteristic and using molecular technique. The result showed that ten isolates belonging to Ascomycota were presumed to be in genus Colletotrichum, Lasiodiplodia and Fusarium in eight isolates and two isolates were identified to class Dothideomycetes and Sordariomycetes. Two unidentified isolates (R14 and R15), confined only to root segments were isolated, these members may be from the group of Mycelia-Sterilia. The evaluation of antagonistic activity of twelve fungal endophytes isolated from healthy Houttuynia cordata Thumb against Trichoderma harzianum and five plant pathogenic fungi (Fusarium oxysporum, Sclerotium rolfsii, Rhizoctonia sp., Alternaria brassicicola and Phytophthora palmivora) by dual culture technique were performed. The in vitro antagonistic activities as two types of activities in this study; mycoparasitism and competition were found. Endophyte isolate B06, B12, B09 and R15 gave the highest percent inhibition of radial growth against to the plant pathogenic fungi (F. oxysporum, S. rolfsii, A. brassicicola) and T. harzianum within 24.22%, 29.07%, 13.54 and 24.15%, respectively. Endophyte isolate R14 showed the highest antagonistic activity to Rhizoctonia sp. and P. palmivora with 39.33% and 33.34% respectively. For the siderophore production, the seven active endophyte isolates showed orange halo formed around the colonies more than 30 mm on CAS agar. Three endophyte isolates efficiently produced IAA more than 24 µg/ml. The active endophyte isolates B06 identified as Lasiodiplodia pseudotheobromae showed the highest ability to produce IAA (37.034 µg/ml) and also showed the highest orange halo formed around the colonies which determined to siderophore production.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"55-66"},"PeriodicalIF":0.0,"publicationDate":"2016-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66677068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ripe palmyra palm or toddy palm ( Borassus flabellifer Linn.) has the yellow-orange pulp with proximate pH 4.47-5.1 and total soluble solid 5.01±0.15 o Brix. In this research, the yeast Candida stellimalicola isolated from the ripe palm fruit pulp was used to ferment the palm fruit juice to ethanol and applied into acetic acid production. The effects of ammonium sulphate as the nitrogen source and sugars (glucose and sucrose) as the carbon source on ethanol fermentation were investigated. The ammonium sulphate concentrations at 300, 500 and 700 mg/L significantly increased the produced ethanol content ( p 0.05). Whereas, using 10 o Brix sucrose showed the ethanol content with 1.82±0.45 and closely to 15 o Brix (1.70±0.41%). For scale up at 6 liters, the fermentation of palmyra palm fruit juice with 10 o Brix glucose and 500 mg/L ammonium sulfate supplementation was carried out at room temperature (31±2 o C) and 300 rpm/min. As the results, the ethanol content was approximately 3.92±0.15% after 14 days. In the future this product will be used for acetic acid production.
成熟的棕榈或棕榈棕榈(Borassus flabellifer Linn.)具有黄橙色的果肉，近似pH值为4.47-5.1，总可溶性固形物为5.01±0.15 o白利度。本研究利用从成熟棕榈果肉中分离得到的念珠菌，将棕榈果汁发酵成乙醇，并应用于醋酸生产。研究了以硫酸铵为氮源，糖(葡萄糖和蔗糖)为碳源对乙醇发酵的影响。300、500和700 mg/L硫酸铵浓度显著提高了产乙醇含量(p < 0.05)。而使用10 o Brix蔗糖时，乙醇含量为1.82±0.45，接近15 o Brix(1.70±0.41%)。在室温(31±2℃)和300 rpm/min的条件下，对棕榈果汁进行发酵，添加10 o Brix葡萄糖和500 mg/L硫酸铵，扩大到6升。结果表明，14 d后乙醇含量约为3.92±0.15%。未来该产品将用于醋酸生产。
{"title":"Preparation the substrate from palmyra palm fruit by candida stellimalicola fermentation for acetic acid production","authors":"S. Artnarong, P. Masniyom, J. Maneesri","doi":"10.14456/KKURJ.2016.43","DOIUrl":"https://doi.org/10.14456/KKURJ.2016.43","url":null,"abstract":"The ripe palmyra palm or toddy palm ( Borassus flabellifer Linn.) has the yellow-orange pulp with proximate pH 4.47-5.1 and total soluble solid 5.01±0.15 o Brix. In this research, the yeast Candida stellimalicola isolated from the ripe palm fruit pulp was used to ferment the palm fruit juice to ethanol and applied into acetic acid production. The effects of ammonium sulphate as the nitrogen source and sugars (glucose and sucrose) as the carbon source on ethanol fermentation were investigated. The ammonium sulphate concentrations at 300, 500 and 700 mg/L significantly increased the produced ethanol content ( p 0.05). Whereas, using 10 o Brix sucrose showed the ethanol content with 1.82±0.45 and closely to 15 o Brix (1.70±0.41%). For scale up at 6 liters, the fermentation of palmyra palm fruit juice with 10 o Brix glucose and 500 mg/L ammonium sulfate supplementation was carried out at room temperature (31±2 o C) and 300 rpm/min. As the results, the ethanol content was approximately 3.92±0.15% after 14 days. In the future this product will be used for acetic acid production.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"21 1","pages":"397-403"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66676936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was designed to evaluate the total phenolic content (TPC), antioxidation, antiproliferation and apoptotic properties of mangosteen ( Garcinia mangostana L.) pericarp ethanolic and water extracts (MPE/e and MPE/w) using human breast adenocarcinoma cell line, MCF-7, as a model system. Folin-Ciocalteu method was used for TPC analysis and DPPH as well FRAP were used for antioxidant capacity. Cell viability was evaluated by Resazurin and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays for 12 and 24 h. The study found that TPC of MPE/e was over than MPE/w (119.95 ± 1.03 > 84.40 ± 0.29 µg GAE/mg, respectively). Consistently, the antioxidant capacity of MPE/e was higher than MPE/w about 2 folds, which expressed via free radical scavenging, IC 50 of 1,199.85 ± 47.16 and 2,435.71 ± 273.74 µg/ml and FRAP, IC 50 of 546.98 ± 26.12 and 1,088.70 ± 81.54 µg/ml. Moreover, only MPE/e expressed as a potential inhibitor on the MCF-7 cells proliferation at dose- and time- dependent manner. MPE/e was strongly positive influence on antiproliferation and apoptotic induction of MCF-7 cells as seen via the morphological changes and oligonucleosomal DNA fragments. In conclusion, MPE/e may be proposed to develop to cytotoxic agents or antitumor if it has been investigated that effective and non-toxic to normal cells of animals and humans.
{"title":"Antioxidant and cytotoxic activities of mangosteen garcinia mangostana pericarp extracts","authors":"Jinnawat Manasathien","doi":"10.14456/KKURJ.2015.32","DOIUrl":"https://doi.org/10.14456/KKURJ.2015.32","url":null,"abstract":"This study was designed to evaluate the total phenolic content (TPC), antioxidation, antiproliferation and apoptotic properties of mangosteen ( Garcinia mangostana L.) pericarp ethanolic and water extracts (MPE/e and MPE/w) using human breast adenocarcinoma cell line, MCF-7, as a model system. Folin-Ciocalteu method was used for TPC analysis and DPPH as well FRAP were used for antioxidant capacity. Cell viability was evaluated by Resazurin and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays for 12 and 24 h. The study found that TPC of MPE/e was over than MPE/w (119.95 ± 1.03 > 84.40 ± 0.29 µg GAE/mg, respectively). Consistently, the antioxidant capacity of MPE/e was higher than MPE/w about 2 folds, which expressed via free radical scavenging, IC 50 of 1,199.85 ± 47.16 and 2,435.71 ± 273.74 µg/ml and FRAP, IC 50 of 546.98 ± 26.12 and 1,088.70 ± 81.54 µg/ml. Moreover, only MPE/e expressed as a potential inhibitor on the MCF-7 cells proliferation at dose- and time- dependent manner. MPE/e was strongly positive influence on antiproliferation and apoptotic induction of MCF-7 cells as seen via the morphological changes and oligonucleosomal DNA fragments. In conclusion, MPE/e may be proposed to develop to cytotoxic agents or antitumor if it has been investigated that effective and non-toxic to normal cells of animals and humans.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"20 1","pages":"381-392"},"PeriodicalIF":0.0,"publicationDate":"2015-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to investigate the effects of dietary supplementing banana as prebiotic on growth performance, carcass characteristics and meat quality of broiler chickens and also to compare them with commercial probiotic and their combination. The experiment was conducted in 200 day-old male chicks. Birds were randomly allocated to 4 treatment groups (50 chicks per treatment). Dietary treatments consisted of basal diet (CON) or the basal diet with 0.5% of banana as prebiotic (PRE), 0.2% of commercial probiotic Toyocerin® (PRO) or 0.7% of their combination (SYN). The results showed that dietary supplementations significantly affected average daily gain and feed conversion ratio of broilers during the period of 0 to 21 days (p 0.05). There were also no significant differences among dietary treatments in meat quality traits, i.e. pH values, drip loss and thawing loss (p>0.05). The results suggested that bananas could be used as a feed additive to improve growth performance of broiler chickens during 1- to 21-d of age without adverse effects on carcass and meat quality.
{"title":"Effects of supplementing banana (Musa spp.) as prebiotic, probiotic (Toyocerin®) and their combination on growth performance, carcass and meat quality in broilers","authors":"et.al Krittaphak Buranawit","doi":"10.14456/KKURJ.2015.35","DOIUrl":"https://doi.org/10.14456/KKURJ.2015.35","url":null,"abstract":"The aim of this study was to investigate the effects of dietary supplementing banana as prebiotic on growth performance, carcass characteristics and meat quality of broiler chickens and also to compare them with commercial probiotic and their combination. The experiment was conducted in 200 day-old male chicks. Birds were randomly allocated to 4 treatment groups (50 chicks per treatment). Dietary treatments consisted of basal diet (CON) or the basal diet with 0.5% of banana as prebiotic (PRE), 0.2% of commercial probiotic Toyocerin® (PRO) or 0.7% of their combination (SYN). The results showed that dietary supplementations significantly affected average daily gain and feed conversion ratio of broilers during the period of 0 to 21 days (p 0.05). There were also no significant differences among dietary treatments in meat quality traits, i.e. pH values, drip loss and thawing loss (p>0.05). The results suggested that bananas could be used as a feed additive to improve growth performance of broiler chickens during 1- to 21-d of age without adverse effects on carcass and meat quality.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"20 1","pages":"419-427"},"PeriodicalIF":0.0,"publicationDate":"2015-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clinical lumbar instability is increasingly recognized as one of the significant causes of chronic low back pain. The patients with clinical lumbar instability require the effective exercise intervention to improve the stability of their lumbar spine. This article reveals the etiology and clinical diagnosis of clinical lumbar instability, the effective treatment as core stabilization exercise (CSE) and also provides some relevant researches on CSE for improving outcome measurements in patients with clinical lumbar instability. Commonly, clinical signs and the specific clinical tests including instability catch sign, apprehension sign, painful catch test and prone instability test are considered as clinical diagnostic criteria due to absence of radiological findings in patients with clinical lumbar instability. Moderate to high impacted evidences on the effectiveness of CSE to improve pain-related outcomes and deep trunk muscle activation have been reported in patients with clinical lumbar instability but further studies regarding long-term effectiveness of CSE and the use of precise instrument to detect deep trunk muscle activation are still required.
{"title":"Clinical Lumbar Instability and Core Stabilization Exercise: A Literature Review","authors":"Pattanasin Areeudomwong","doi":"10.14456/KKURJ.2015.39","DOIUrl":"https://doi.org/10.14456/KKURJ.2015.39","url":null,"abstract":"Clinical lumbar instability is increasingly recognized as one of the significant causes of chronic low back pain. The patients with clinical lumbar instability require the effective exercise intervention to improve the stability of their lumbar spine. This article reveals the etiology and clinical diagnosis of clinical lumbar instability, the effective treatment as core stabilization exercise (CSE) and also provides some relevant researches on CSE for improving outcome measurements in patients with clinical lumbar instability. Commonly, clinical signs and the specific clinical tests including instability catch sign, apprehension sign, painful catch test and prone instability test are considered as clinical diagnostic criteria due to absence of radiological findings in patients with clinical lumbar instability. Moderate to high impacted evidences on the effectiveness of CSE to improve pain-related outcomes and deep trunk muscle activation have been reported in patients with clinical lumbar instability but further studies regarding long-term effectiveness of CSE and the use of precise instrument to detect deep trunk muscle activation are still required.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"20 1","pages":"465-479"},"PeriodicalIF":0.0,"publicationDate":"2015-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Traffic congestion to inner Bangkok needs to be relieved by some kind of a toll charge. The paper investigated whether and to what level the congestion charging will help to solve the congestion problem of inner Bangkok. A travel demand model is estimated and the factors influencing the driving demand for inner Bangkok, where most intensively used mass transit network exists during the years 2012-2014, are reported. The objective is to understand the drivers and investigate the degree of decreased traffic if the congestion charging scheme is implemented and also report other relevant variables. The results show that such the reported acceptable charge has a significant statistically impact on the demand with increasing degree of impact as well as other price-related variable such as fuel costs. Quantity effects such as higher income, more cars in household, more travel time spent to work, and having a parking space at office are used to increase the demand; however, these quantity factors do not have impact on the demand. Conversely, the number of dropping person used to has no impact. Spatial effects, such as the distance from home to office, the distance from office to the metro station, and price effects such as parking fees are not statistically significant. Some personnel factors were not relevant to changes in the number of trips but recently they are reducing factors; these are age, education and urgency of the trip. Factors such as toll charge, income level number of cars in household, travel time to work and parking space are factors increasing the demand. Fuel costs and the occupation officer lower the travel demand.
{"title":"Trends of driving demand to inner Bangkok in situation of a congestion charging","authors":"et.al Muanmas Wichiensin","doi":"10.14456/KKURJ.2015.36","DOIUrl":"https://doi.org/10.14456/KKURJ.2015.36","url":null,"abstract":"Traffic congestion to inner Bangkok needs to be relieved by some kind of a toll charge. The paper investigated whether and to what level the congestion charging will help to solve the congestion problem of inner Bangkok. A travel demand model is estimated and the factors influencing the driving demand for inner Bangkok, where most intensively used mass transit network exists during the years 2012-2014, are reported. The objective is to understand the drivers and investigate the degree of decreased traffic if the congestion charging scheme is implemented and also report other relevant variables. The results show that such the reported acceptable charge has a significant statistically impact on the demand with increasing degree of impact as well as other price-related variable such as fuel costs. Quantity effects such as higher income, more cars in household, more travel time spent to work, and having a parking space at office are used to increase the demand; however, these quantity factors do not have impact on the demand. Conversely, the number of dropping person used to has no impact. Spatial effects, such as the distance from home to office, the distance from office to the metro station, and price effects such as parking fees are not statistically significant. Some personnel factors were not relevant to changes in the number of trips but recently they are reducing factors; these are age, education and urgency of the trip. Factors such as toll charge, income level number of cars in household, travel time to work and parking space are factors increasing the demand. Fuel costs and the occupation officer lower the travel demand.","PeriodicalId":8597,"journal":{"name":"Asia-Pacific Journal of Science and Technology","volume":"20 1","pages":"428-448"},"PeriodicalIF":0.0,"publicationDate":"2015-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66675875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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