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Association between Serum C-peptide Level and Diabetic Nephropathy in Type 2 Diabetes Mellitus Patients on Oral Anti-diabetic Drug 口服降糖药2型糖尿病患者血清c肽水平与糖尿病肾病的关系
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-07 DOI: 10.3329/bjms.v22i4.67106
Adhir Kumar Das, R. Tasnim, Jahidul Islam
Background: Diabetic Nephropathy (DN) treatment focuses mostly on preventing or delaying disease development. The most recent research indicates that C-peptide may have a favourable biological effect on DN. In type 2 Diabetes Mellitus, the link between C-peptide level and DN is poorly understood (DM). The purpose of this study is to evaluate the relation between serum C-peptide and diabetic nephropathy in type 2 DM patients taking an oral anti diabetic medication.Methods: From July 2019 to June 2020, a cross-sectional observational study was done at the Department of Pharmacology and Therapeutics at Dhaka Medical College, Dhaka. A total of 63 randomly selected type 2 diabetes patients met the inclusion criteria. SPSS was used to collect, record, and evaluate biochemical values including FBG, 2hrs ABF, HbA1c, urine for microalbumin, serum creatinine, and serum C-peptide.Result: Among 63 study participants, 40 (63.5%) were female and 23 (36.5%) were male. Mean age of patients was 50.30±10.55 years. Mean duration of DM of total study subjects was 6.29±3.15 years. Out of total study subjects, 17.5% (11) had low serum C-peptide, 74.6% (47) had normal serum C-peptide and 7.9% (5) had high serum C-peptide level. Correlations of clinical and biochemical variables showed negative correlation between serum C-peptide level and microalbumin, DM duration, FBS, 2hrs ABF, HbA1c, creatinine. Mean urine albumin was higher in patients with low serum C-peptide level. The microalbumin level and the duration of DM of study subjects are significantly associated with serum C-peptide.Conclusion: In this study we have found that type 2 diabetic individuals with low serum C-peptide levels have a high frequency of diabetic nephropathy and that serum C-peptide levels are significantly associated with diabetic nephropathy.Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 768-772
背景:糖尿病肾病(DN)的治疗主要集中在预防或延缓疾病的发展。最近的研究表明,c肽可能对DN有良好的生物学作用。在2型糖尿病中,c肽水平与DN之间的关系尚不清楚(DM)。本研究旨在探讨口服抗糖尿病药物的2型糖尿病患者血清c肽与糖尿病肾病的关系。方法:2019年7月至2020年6月,在达卡医学院药理学和治疗学系进行了一项横断面观察研究。随机选择63例符合纳入标准的2型糖尿病患者。使用SPSS软件收集、记录和评价生化指标,包括空腹血糖、2hrs ABF、糖化血红蛋白、尿微量白蛋白、血清肌酐和血清c肽。结果:63名研究对象中,女性40人(63.5%),男性23人(36.5%)。患者平均年龄50.30±10.55岁。研究对象DM的平均病程为6.29±3.15年。在所有研究对象中,17.5%(11人)血清c肽水平低,74.6%(47人)血清c肽水平正常,7.9%(5人)血清c肽水平高。血清c肽水平与微量白蛋白、糖尿病病程、FBS、2hrs ABF、HbA1c、肌酐呈负相关。血清c肽水平低的患者平均尿白蛋白较高。研究对象的微量白蛋白水平和糖尿病持续时间与血清c肽显著相关。结论:本研究发现血清c肽水平低的2型糖尿病患者糖尿病肾病发生率高,且血清c肽水平与糖尿病肾病有显著相关性。孟加拉国医学杂志,第22卷第4期,23年10月,第768-772页
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引用次数: 0
Diverted Mini Gastric Bypass Surgery in Obese Population: Out-turn Over Hepatic and Nephrological Parameters 肥胖人群的分流胃旁路手术:肝脏和肾脏参数的结果
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-07 DOI: 10.3329/bjms.v22i4.68669
Arya Singh, Rahnuma Ahmad, Susmita Sinha, Md. Ahsanul Haq, M. Narwaria, Mainul Haque, Santosh Kumar, Nandita Sanghani
Introduction: Bariatric surgery is the best possible option for reducing weight when lifestyle changes and medication have not brought about lasting success and may reduce morbidity and mortality. Weight loss that may follow bariatric surgery in patients on calory restricted diet would result in reduced inflammation and therefore lowering inflammation-related organ damage, including that of the kidney and liver. The study aimed to observe the consequences of BMI change on Hepatic and Nephrological parameters in patients after One Anastomosis Gastric Bypass surgery. Method: This study was done at a bariatric center with 150 individuals (both male and female) aged 20 to 60 years with obesity grades II and III who were selected randomly. Hepatic and renal function tests were carried out at baseline visit, then 3 months and 6 months following surgically. Result: Bilirubin level significantly increased from baseline to visit 1; Serum glutamic-pyruvic transaminase (SGPT) levels significantly decreased at visits 1 and 2. The albumin to Globulin ratio was significantly increased at visit 2 . Blood Urea level and serum creatinine level reduced considerably at visit 1 and decreased more at visit 2 from baseline. Conclusion: Bariatric surgery may be related to improvement in both hepatic and renal function. The improvement may be attributed to reduced inflammatory organ damage related to obesity. More such studies must be performed to highlight the possible health benefits of bariatric surgery for obese patients whose lifestyle modification and medication have not aided in weight loss. Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 743-758
简介:当生活方式的改变和药物治疗不能带来持久的成功时,减肥手术是减肥的最佳选择,可以降低发病率和死亡率。在热量限制饮食的患者进行减肥手术后,体重可能会减轻,从而减少炎症,从而降低与炎症相关的器官损伤,包括肾脏和肝脏。本研究旨在观察一次吻合胃旁路手术后BMI变化对患者肝脏和肾脏参数的影响。方法:本研究在一个减肥中心进行,随机选择年龄在20 - 60岁的II级和III级肥胖患者150人(男女)。在基线访问时进行肝肾功能检查,然后在手术后3个月和6个月进行肝肾功能检查。结果:第一次就诊时胆红素水平明显升高;血清谷丙转氨酶(SGPT)水平在第1次和第2次就诊时显著降低。就诊2时,白蛋白与球蛋白比值显著升高。血液尿素水平和血清肌酐水平在第一次访问时显著下降,在第二次访问时从基线下降更多。结论:减肥手术可能与肝肾功能的改善有关。这种改善可能归因于与肥胖有关的炎症性器官损伤减少。必须进行更多这样的研究,以强调减肥手术对那些生活方式改变和药物治疗对减肥没有帮助的肥胖患者可能带来的健康益处。孟加拉国医学杂志,第22卷第4期,23年10月,第743-758页
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引用次数: 0
Quality of life change seen after Diverted Mini Gastric Bypass Surgery in Obese Population: Retrospective research analysis 肥胖人群分流胃旁路手术后生活质量的改变:回顾性研究分析
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-07 DOI: 10.3329/bjms.v22i4.68665
Arya Singh, M. Narwaria, Prachi Patel, Susmita Sinha, Rahnuma Ahmad, Mainul Haque, Santosh Kumar, Nandita Sanghani
Background: With an increase in life expectancy all over the globe, morbid obesity and obesity-related diseases negatively impact the overall quality of life (QoL). Aim: The study aimed to assess the QoL post-Diverted Mini Gastric Bypass (dMGB), focusing on weight loss achieved, comorbidity resolution, and change in QoL after the surgery. Setting: Single private institute, India Methods : Data from patients who underwent a laparoscopic primary dMGB from August 2020 to August 2021 by one surgeon were retrospectively analyzed. The patients were followed up at 3,6,9, and 12 months from the surgery date. Data were assessed using the Bariatric Analysis and Reporting Outcome System- II (BAROS- II) form based on three major areas: percentage weight loss, comorbidity resolution, and QoL. The variation in QoL was assessed using the Moorehead Ardelt QoL questionnaire, which addresses self-esteem, physical activity, social gathering, and sexual life. Results: Forty-one patients were included in the final analysis. The average age and pre-operative body mass index (BMI) was 45.5 yrs. and 44.5 kg/m2, respectively. The cohort had 60.9% of males and 39.1% females. The pre-operative average total QoL score was 0.65 ± 0.2. At 12 months, the average BMI and average total QoL were 28.03 kg/m2 and 5.35 ± 0.3, respectively, p<0.001. Conclusion: At one year, the dMGB surgery effectively improves the overall QoL. Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 759-767
背景:随着全球预期寿命的增加,病态肥胖和肥胖相关疾病对整体生活质量(QoL)产生了负面影响。目的:本研究旨在评估转移后的迷你胃旁路术(dMGB)后的生活质量,重点关注体重减轻,合并症的解决和术后生活质量的变化。方法:回顾性分析2020年8月至2021年8月由一名外科医生接受腹腔镜原发性dMGB手术的患者的数据。术后随访时间分别为3、6、9、12个月。使用体重分析和报告结果系统- II (BAROS- II)表格对数据进行评估,基于三个主要领域:体重减轻百分比、合并症解决和生活质量。生活质量的变化使用Moorehead Ardelt生活质量问卷进行评估,该问卷涉及自尊,身体活动,社交聚会和性生活。结果:41例患者纳入最终分析。平均年龄和术前体重指数(BMI)为45.5岁。和44.5 kg/m2。该队列中男性占60.9%,女性占39.1%。术前平均总生活质量评分为0.65±0.2。12个月时,平均BMI和平均总生活质量分别为28.03 kg/m2和5.35±0.3,p<0.001。结论:一年时,dMGB手术能有效提高患者的总体生活质量。孟加拉国医学杂志,第22卷第4期,23年10月,第759-767页
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引用次数: 0
Report on the earthquake in Turkey: Field observation on healthcare services and ethical challenges 关于土耳其地震的报告:对保健服务和道德挑战的实地观察
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-07 DOI: 10.3329/bjms.v22i4.68677
M. Değer, Ahmet Özdinç
On February 6, 2023, an earthquake with a magnitude of 7.7 m occurred in Turkey, centered in the Pazarcık district of Kahramanmaraş province, followed nine hours later by another earthquake with a magnitude of 7.6 m, centered in the Elbistan district of Kahramanmaraş province. In the earthquakes, 45,000 people died, and more than 100,000 were injured. During the earthquake search-and-rescue personnel, emergency responders, and volunteers worked in the field. Disasters that suddenly affect large masses of people create many issues related to medical ethics. Likewise, difficulties caused by limited resources lead to issues related to beneficence, non-maleficence, autonomy and justice. This is a descriptive research study based on the field observations of researchers who were actually in the disaster area after the earthquakes, as well as an analysis of and observations related to the news, information and reports published. This study draws attention to ensuring the participation of society in the fight against and response to disasters. In addition, ethical guidelines for healthcare providers in disasters should be prepared and integrated into national disaster plans. Turkey’s earthquake experience will provide essential information for decision-makers and practitioners regarding the extent of the area impacted and the scope of the affected population. Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page :815-826
2023年2月6日,土耳其发生了震级7.7米的地震,震中位于kahramanmarakh省Pazarcık地区,9小时后又发生了震级7.6米的地震,震中位于kahramanmarakh省的Elbistan地区。地震造成4.5万人死亡,10万多人受伤。地震期间,搜救人员、应急人员和志愿者在现场工作。灾难突然影响到大量人群,产生了许多与医学伦理有关的问题。同样,有限的资源所造成的困难导致了与慈善、无害、自治和正义有关的问题。这是一项描述性研究,基于震后实际在灾区的研究人员的实地观察,以及对发表的相关新闻、信息和报道的分析和观察。这项研究提请注意确保社会参与抗灾和救灾工作。此外,应编制灾害中医疗保健提供者的道德准则,并将其纳入国家灾害计划。土耳其的地震经验将为决策者和从业人员提供有关受影响地区的程度和受影响人口范围的重要信息。孟加拉国医学杂志,第22卷第4期,23年10月,第815-826页
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引用次数: 0
Irritation test and melanin whitening cream epigallocatechin gallate green tea leaf with mexameter and self-assessment 没食子儿茶素没食子酸酯绿茶叶黑色素美白霜及刺激试验
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-07 DOI: 10.3329/bjms.v22i4.68678
Naniek Widyaningrum, Fildza Huwaina Fathnin, Yuyun Darma Ayu N, Bella N Sari
Background: Green tea (Camelia sinensis L.) contains flavonoids consisting of Epigallocatechin gallate (EGCG) which functions as whitening, anti-acne, antioxidant, anti-inflammatory. Previous studies have shown that EGCG with a concentration of 6% is the optimum concentration in cream formulations (Widyaningrum, 2015b). Phase One Clinical Test was conducted to determine the safety of cream preparations with irritation as a benchmark and continued to test its effectiveness as a whitening cream on 20 respondents aged 17-45 years. Material and Methods: The research method employed was the Open Test. The results of the study for 21 days after applying the EGCG anti-acne cream were assessed using a mexa-meter and self-assessment. Result: The erythema assessment showed that the preparation did not cause irritation in the absence of erythema and for the melanin assessment, the results were not significant with p Value 0.376 (<0,05) with no changes in the melanin index. The results of the irritation test were supported by the findings of self-assessment which revealed that all self-assessment parameters were not significant and there was no irritation (p 0,317). Conclusion: Based on the results of the study, it could be concluded that EGCG whitening cream did not cause irritation for 21 days. The results of the melanin test formula for this whitening cream preparation were able to provide a skin whitening effect. Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 902-906
背景:绿茶(Camelia sinensis L.)含有由表没食子儿茶素没食子酸酯(EGCG)组成的类黄酮,具有美白、抗痘、抗氧化、抗炎的作用。先前的研究表明,EGCG浓度为6%是乳霜配方的最佳浓度(Widyaningrum, 2015b)。第一阶段临床试验以刺激性为基准确定霜制剂的安全性,并对20名年龄在17-45岁的受访者继续测试其作为美白霜的有效性。材料与方法:研究方法为开放试验。使用EGCG抗痤疮霜后21天的研究结果采用测量仪和自我评估进行评估。结果:红斑评估显示,制剂在无红斑的情况下没有引起刺激;黑色素评估结果无统计学意义(p值0.376(< 0.05)),黑色素指数无变化。自我评估的结果支持了刺激测试的结果,自我评估的所有参数都不显著,没有刺激(p . 0,317)。结论:根据研究结果,可以得出EGCG美白霜在21天内没有引起刺激的结论。该美白霜制剂的黑色素测试公式的结果能够提供皮肤美白效果。孟加拉国医学杂志,第22卷第4期,23年10月,第902-906页
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引用次数: 0
Cervical Cystic Lymphangioma, Rarely Detected in Adults with Rapid Progression, 2 Case Reports 宫颈囊性淋巴管瘤,罕见于成人且进展迅速,附2例报告
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-06-16 DOI: 10.3329/bjms.v22i3.65318
Fazlı Yanık, Y. Karamustafaoğlu, Y. Yoruk
Cystic Lymphangioma(CL) is rarely detected in adulthood. We presented two cases with neck swelling at an advanced age. Both of cysts were located in the cervico-mediastinally and showed rapid progression. The cysts were excised successfully and there was no recurrence in the follow-up period. Although there are many alternatives in treatment, CL, which is rare in adults, can be treated with surgical excision successfully in appropriate cases to definitively prevent recurrence and complications.Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 709-711
囊性淋巴管瘤(CL)在成人中很少被发现。我们提出了两例颈部肿胀在老年。两个囊肿均位于颈纵隔,且进展迅速。囊肿成功切除,随访期间无复发。虽然有许多治疗方法可供选择,但在成人中罕见的CL可以在适当的情况下通过手术切除成功治疗,以明确预防复发和并发症。《孟加拉国医学科学杂志》第22卷第23年7月3日第709-711页
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引用次数: 0
Development of gamification as addiction education and examining its effects on students’ motivation and enjoyment 游戏化成瘾教育的发展及其对学生动机和享受的影响
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-06-16 DOI: 10.3329/bjms.v22i3.65325
L. Mosalanejad, Erfan Mansouri
Introduction Addiction is a “social disease” which has physical and mental effects. Having adequate knowledge and information about addiction is the best way to prevent it in the community. The current study aims to focus on developing gamification as addiction education and examining its effects on students’ motivation and enjoyment.Methods: This is guise experiment for gamification development and assess educational intervention. Educational and technical standards have been considered for the development of gamification. In technical standards, it is congruent with the app-making technology based on dynamism, mechanisms and dynamic gamification. This game is available on www.addiplay.ir website through Microsoft Aps net core technology. Educational intervention implied on two groups of medical students and Public Health students and learning indicators assessed through intervention.Results; The findings of this study on two groups of students showed that, the game can influence on students’ enjoyment and consent. In addition, the game had a high average in increasing learners’ ability and students satisfaction. One of the limitations of gamification has been its dependence on each learner’s ability.Conclusion: The results showed that new teaching and learning methods such as gamification in addiction lessons in a mixed and complimentary way can fulfill the educational goals instead of written handbooks or traditional classes and makes learning possible in a motivated and entertaining environment.Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 564-572
成瘾是一种对身心都有影响的“社会疾病”。对成瘾有足够的了解和信息是在社区中预防成瘾的最好方法。目前的研究旨在将游戏化发展为成瘾教育,并研究其对学生动机和享受的影响。方法:对游戏化发展进行伪装实验,评估教育干预。教育和技术标准已经被考虑到游戏化的发展。在技术标准上,它与基于动态性、机制性和动态游戏化的app制作技术是一致的。本游戏通过微软Aps网核心技术在www.addiplay.ir网站上发布。两组医学生和公共卫生专业学生隐含的教育干预以及通过干预评估的学习指标。本研究对两组学生的研究结果表明,游戏可以影响学生的享受和同意。此外,该游戏在提高学习者能力和学生满意度方面具有较高的平均水平。游戏化的局限性之一是它依赖于每个学习者的能力。结论:在成瘾课程中引入游戏化等新的教学方法,以混合和互补的方式代替书面手册或传统课堂,可以实现教育目标,使学习成为可能,在一个积极和有趣的环境中。《孟加拉国医学科学杂志》第22卷第23年7月3日第564-572页
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引用次数: 1
The influence of physical activity on the thinking of schoolchildren 体育活动对小学生思维的影响
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-06-16 DOI: 10.3329/bjms.v22i3.65334
G. Polevoy
Objective: to determine the influence of physical activity in physical education classes on the thinking indicators of schoolchildren.Methods: This was a cross-sectional study conducted in a secondary school in Russia, it was attended by ninth graders aged 15-16 years in the number of 141 schoolchildren. The main method of research was the test “Cross out an extra word”, which determines the level of development of students’ thinking. The test was used before and after the physical education lesson 1 time per month for 5 months. Statistical analysis used: The student’s T-criterion was used in the study.Results: children who did not engage in physical education in the classroom were not able to significantly improve their performance in the test, which indicates a possible adaptation to the test after its first performance before the lesson. Children who were engaged in physical exercises were able to significantly increase the test results.Conclusion: the effectiveness of the influence of a physical education lesson at school on thinking indicators. This study will serve as an additional motivation for children to engage in physical culture, since the impact of physical exercises has a positive impact not only on the development of physical qualities, but also on the development of schoolchildren’s thinking.Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 612-616
目的:了解体育课体育活动对小学生思维指标的影响。方法:本研究是在俄罗斯一所中学进行的横断面研究,对象为15-16岁的九年级学生141名。研究的主要方法是“划掉一个多余的单词”测试,它决定了学生思维的发展水平。测试在体育课前后进行,每月1次,持续5个月。采用统计分析:采用学生t标准。结果:没有在课堂上进行体育教育的孩子在测试中的表现并没有明显的提高,这表明可能是在上课前的第一次表现后对测试的适应。参加体育锻炼的孩子能够显著提高测试结果。结论:学校体育课对思维指标影响的有效性。本研究将作为儿童从事体育活动的额外动机,因为体育锻炼的影响不仅对身体素质的发展有积极的影响,而且对小学生思维的发展也有积极的影响。《孟加拉国医学科学杂志》第22卷第23年7月3日第612-616页
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引用次数: 0
Roles of intrinsic and acquired resistance determinants in multidrug-resistant clinical Pseudomonas aeruginosa in Bangladesh 孟加拉国临床多药耐药铜绿假单胞菌内在和获得性耐药决定因素的作用
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-06-16 DOI: 10.3329/bjms.v22i3.66960
Hasnain Anjum, Md. Shamsul Arefin, Nusrat Jahan, Mumtarin Jannat Oishee, S. Nahar, Salequl Islam, S. Banerjee, Susmita Sinha, Santosh Kumar, Mainul Haque, M. H. Rahman
Introduction: Pseudomonas aeruginosa is an ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, P. aeruginosa, and Enterobacter spp.) pathogen and one of the leading etiologies in multiple nosocomial infections. Treatment of P. aeruginosa is becoming increasingly difficult due to its ever-increasing antibiotic resistance trends. This study investigated clinical multidrug resistance (MDR) P. aeruginosa (MDR-PA), their intrinsic resistance determinants, including the presence of chromosomal AmpC β-lactamase (Ampicillinase), decreased expression of outer membrane porin protein OprD and selected acquired β-lactamase resistance genes. Methods: Out of 238 clinical specimens, including urines from urinary tract-infected patients, wound swabs, burn swabs, and catheter aspirates, were collected from two major hospitals in Savar, Dhaka, Bangladesh. Samples were inoculated with Cetrimide agar to isolate presumptive P. aeruginosa. Bacteria were identified by cultural, biochemical characterization, 16S rDNA sequencing, and phylogenetic analysis. Virulence-associated genes of P. aeruginosa, namely, toxA, lasB, and plcH, were identified by polymerase chain reaction (PCR). Antibiotic susceptibilities of the isolates were investigated against ten antibiotics belonging to seven groups by disc-diffusion method followed by a selected minimum inhibitory concentration (MIC) assay. Phenotypic expression of Metallo-β-lactamases (MBLs) production was checked by the double disc synergistic test selectively among the imipenem-resistant isolates. Acquisition of β-lactam resistance trait was examined by PCR detection of bla-genes variants. Mutational loss of the OprD was analyzed by PCR to investigate intrinsic resistance determinants. Phenotypic overexpression of chromosomal AmpC was assayed with the identification of the AmpC gene by PCR. The expression level of OprD was assessed by real-time quantitative PCR (RT-qPCR). Results: Fifty-three P. aeruginosa was identified, with an overall isolation of 22.3% (53/238), where urine remains the most prevalent source. Virulence genes toxA, lasB, and plcH were identified in the isolates of 92.4%, 96.2%, and 94.3%. The highest phenotypic antimicrobial resistance was observed against ampicillin and ceftriaxone (100%), followed by cefotaxime (96%), tetracycline (89%), azithromycin (72%), imipenem (31%), ciprofloxacin (29%), levofloxacin (29%), gentamycin (27%) and ceftazidime (14%). The antibiogram pattern revealed 85% of isolates as multidrug-resistant, while 12% were considered extensively drug-resistant (XDR)-P. aeruginosa. The carriage of β-lactamase genes blaTEM, blaSHV, and blaOXA was detected in 4%, 2%, and 2% cephalosporin-resistant isolates, respectively. Double disc synergistic test revealed 87% of imipenem-resistant isolates expressing MBL-mediated resistance phenomenon. All seven ceftazidime-resistant isolates showed the presence of the AmpC gene with phenotypic overproduct
铜绿假单胞菌是一种ESKAPE(粪肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌)病原体,也是多种医院感染的主要病因之一。铜绿假单胞菌的治疗变得越来越困难,由于其不断增加的抗生素耐药性趋势。本研究研究了临床多药耐药P. aeruginosa (MDR- pa)及其内在耐药决定因素,包括染色体AmpC β-内酰胺酶(氨苄青霉素酶)的存在、外膜孔蛋白OprD的表达降低以及获得性β-内酰胺酶耐药基因的选择。方法:238份临床标本,包括尿路感染患者的尿液、伤口拭子、烧伤拭子和导尿管抽吸物,采集于孟加拉国达卡萨瓦尔市两家大医院。样品接种了Cetrimide琼脂,以分离推定的铜绿假单胞菌。通过培养、生化鉴定、16S rDNA测序和系统发育分析对细菌进行鉴定。采用聚合酶链反应(PCR)鉴定了铜绿假单胞菌毒力相关基因toxA、lasB和plcH。采用圆盘扩散法测定菌株对7类10种抗生素的敏感性,并采用最小抑菌浓度(MIC)测定。采用双盘增效试验对耐亚胺培南菌株产生金属β-内酰胺酶(MBLs)的表型表达进行了选择性检测。通过PCR检测β-内酰胺基因变异,检验β-内酰胺抗性性状的获得。通过PCR分析OprD的突变损失,以研究内在抗性决定因素。用PCR方法鉴定AmpC基因,检测AmpC染色体表型过表达。实时荧光定量PCR (RT-qPCR)检测OprD的表达水平。结果:共鉴定出53株铜绿假单胞菌,总分离率为22.3%(53/238),其中尿液仍然是最常见的来源。毒力基因toxA、lasB和plcH分别为92.4%、96.2%和94.3%。表型耐药率最高的是氨苄西林和头孢曲松(100%),其次是头孢噻肟(96%)、四环素(89%)、阿奇霉素(72%)、亚胺培南(31%)、环丙沙星(29%)、左氧氟沙星(29%)、庆大霉素(27%)和头孢他啶(14%)。抗生素谱模式显示85%的分离株具有多重耐药,而12%的分离株被认为具有广泛耐药(XDR)-P。绿脓杆菌。在4%、2%和2%的头孢菌素耐药菌株中分别检测到β-内酰胺酶基因blaTEM、blaSHV和blaOXA的携带。双盘协同试验显示87%的亚胺培南耐药菌株表达mbl介导的耐药现象。所有7株头孢他啶耐药菌株均存在AmpC基因,且AmpC酶表型过量产生,表明AmpC介导头孢他啶耐药。在12%的表型多药耐药分离株中观察到OprD突变缺失,RT-qPCR分析显示,多药耐药分离株外膜中不同水平的OprD孔蛋白表达降低。结论:本研究描述了孟加拉国临床标本中耐多药pa的高患病率。已确定的内在和获得性抗菌素耐药性决定因素在新出现的耐多药耐药性中发挥协同作用。《孟加拉国医学科学杂志》第22卷第23年7月3日第489-507页
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引用次数: 0
A comparative study of the effectiveness of Pfizer-BioNTech (BNT162b2), Astra Zeneca (ChAdOx1nCoV-19) and Sinopharm (BBIBP-CorV) vaccines in eliciting Humoral immunity in a sample of vaccinated population from Iraq. 辉瑞- biontech (BNT162b2)、阿斯特拉-利康(ChAdOx1nCoV-19)和国药(BBIBP-CorV)疫苗在伊拉克接种人群中激发体液免疫效果的比较研究
IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-06-16 DOI: 10.3329/bjms.v22i3.65323
Furqan Mohammed, A. Sahib
Background: In order to tackle COVID-19 pandemic and the emerging variants, researchers around the globe have investigated many vaccine candidates from different manufacturers, however vaccine development is not an easy task but is a top priority to restore normalcy as represented a step to achieve the desired herd immunity threshold.Patients and methods: in this study we assessed and compared the level of IgG anti-RBD neutralizing antibodies triggered from each vaccine against SARS-CoV2 infection in 123 vaccinated subjects, by using isotypeand species- free competitive blocking ELISA. Blood samples were taken from vaccinated individuals 1 and 8 months after the second dose of the vaccines.Results: the findings of the current study revealed that two-dose vaccination might be effective to trigger robust humoral neutralizing immunity at 1month and even durable for as long as 8months with different sustained levels among the three studied previously mentioned vaccines. The serum level of the neutralizing IgG antibodies, Pfizer group revealed the highest level compared to AstraZeneca and Sinopharm groups (P<0.05); the Sinopharm showed trend of higher levels of neutralizing antibodies than AstraZeneca but without reaching statistical significance (P>0.05). Additionally, the serum level of neutralizing IgG antibodies, which represent the humeral immunity to SARSCoV- 2, was shown to be far higher in 1-month than in 8-month post-2nd dose vaccination groups (P<0.0001).Conclusion: Altogether, it is concluded that Pfizer vaccine proved to be of highest and most durable neutralizing anti-RBD IgG antibodies and followed with Sinopharm and AstraZeneca vaccines.Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 553-563
背景:为了应对COVID-19大流行和新出现的变体,全球研究人员已经研究了来自不同制造商的许多候选疫苗,然而疫苗开发不是一项容易的任务,而是恢复正常的首要任务,因为它代表了实现所需群体免疫阈值的一步。患者和方法:在本研究中,我们采用同种型和无种竞争性阻断ELISA法,评估和比较了123名接种疫苗的受试者中每种针对SARS-CoV2感染的疫苗触发的IgG抗rbd中和抗体水平。在接种第二剂疫苗后1个月和8个月采集了接种者的血液样本。结果:目前的研究结果表明,两剂疫苗接种可能有效地在1个月时触发强大的体液中和免疫,甚至持续长达8个月,但上述三种疫苗的持续水平不同。血清中和性IgG抗体水平,辉瑞组高于阿斯利康组和国药组(P0.05)。此外,血清中和性IgG抗体(代表肱骨对SARSCoV- 2的免疫)水平在第二次接种后1个月远高于第二次接种后8个月(P<0.0001)。结论:综上所述,辉瑞疫苗具有最高和最持久的抗rbd IgG抗体,其次是国药和阿斯利康疫苗。《孟加拉国医学科学杂志》第22卷第23年7月3日第553-563页
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引用次数: 0
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Bangladesh Journal of Medical Science
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