Hong-jun Song, Xuxiao Ye, T. Liang, Dongliang Yan, Zuowei Li
The present study was undertaken to decipher the role of HOXC10 gene in regulating the growth and metastasis of prostate cancer. The results revealed significant (p<0.05) up-regulation of HOXC10 gene in human prostate cancer tissues and cell lines. The silencing of HOXC10 transcript level significantly (p<0.05) inhibited the growth and colony formation of DU145 and 22Rv1 prostate cancer cells. The DAPI staining revealed that inhibition of DU145 and 22Rv1 prostate cancer cell viability was due to the induction of apoptosis. The transwell assay showed that HOXC10 significantly (p<0.05) inhibits the invasion of prostate cancer cells. The Western blotting revealed that HOXC10 gene exerts its effects via modulation of Ras/Raf/MEK/ERK signaling cascade. Collectively, the results point towards the therapeutic potential of HOXC10 in the treatment of prostate cancer.
{"title":"Expression of HOXC10 gene regulates the growth and invasion of prostate cancer cells","authors":"Hong-jun Song, Xuxiao Ye, T. Liang, Dongliang Yan, Zuowei Li","doi":"10.3329/BJP.V16I1.50244","DOIUrl":"https://doi.org/10.3329/BJP.V16I1.50244","url":null,"abstract":"The present study was undertaken to decipher the role of HOXC10 gene in regulating the growth and metastasis of prostate cancer. The results revealed significant (p<0.05) up-regulation of HOXC10 gene in human prostate cancer tissues and cell lines. The silencing of HOXC10 transcript level significantly (p<0.05) inhibited the growth and colony formation of DU145 and 22Rv1 prostate cancer cells. The DAPI staining revealed that inhibition of DU145 and 22Rv1 prostate cancer cell viability was due to the induction of apoptosis. The transwell assay showed that HOXC10 significantly (p<0.05) inhibits the invasion of prostate cancer cells. The Western blotting revealed that HOXC10 gene exerts its effects via modulation of Ras/Raf/MEK/ERK signaling cascade. Collectively, the results point towards the therapeutic potential of HOXC10 in the treatment of prostate cancer.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"16 1","pages":"19-26"},"PeriodicalIF":1.6,"publicationDate":"2021-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49402510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was designed to reintroduce an older antimicrobial nitrofurantoin among a group of prescribers and to explore the relationship between the reintroduction and corresponding shift in sensitivity pattern of the microbes. The microbial sensitivity against ciprofloxacin before intervention was 46.6%, which significantly increased after intervention (51.8%). Treatment with nitrofurantoin resulted in a total clinical cure rate of 80.6%, of which rate of clinical cure achieved in expected time was 66.1% and delayed was 14.5%. The rate of total clinical cure with nitrofurantoin corresponded closely with the proportion of urinary isolates sensitive to nitrofurantoin. i.e., 86.3%. This preliminary study revealed that revival might be an effective strategy to increase the sensitivity and to delay the emergence of resistance.
{"title":"Reintroduction of nitrofurantoin in the treatment of uncomplicated urinary tract infection","authors":"Sabiha Mahboob, M. Rahman","doi":"10.3329/bjp.v16i3.54481","DOIUrl":"https://doi.org/10.3329/bjp.v16i3.54481","url":null,"abstract":"The present study was designed to reintroduce an older antimicrobial nitrofurantoin among a group of prescribers and to explore the relationship between the reintroduction and corresponding shift in sensitivity pattern of the microbes. The microbial sensitivity against ciprofloxacin before intervention was 46.6%, which significantly increased after intervention (51.8%). Treatment with nitrofurantoin resulted in a total clinical cure rate of 80.6%, of which rate of clinical cure achieved in expected time was 66.1% and delayed was 14.5%. The rate of total clinical cure with nitrofurantoin corresponded closely with the proportion of urinary isolates sensitive to nitrofurantoin. i.e., 86.3%. This preliminary study revealed that revival might be an effective strategy to increase the sensitivity and to delay the emergence of resistance.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"45 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69467046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antimicrobial activity of volatile compounds produced by endophytic fungi associated with Ocimum sanctum","authors":"M. T. M. Jalil, D. Ibrahim","doi":"10.3329/bjp.v15i4.48496","DOIUrl":"https://doi.org/10.3329/bjp.v15i4.48496","url":null,"abstract":"","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44618272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Abdur Rehman Shah, R. Khan, Mushtaq Ahmed
The methanolic extract of Iphiona aucheri leaves and its n-hexane, chloroform and aqueous fractions were screened for phytochemicals, cytotoxic effects, total phenolic contents, antioxidant and anti-diabetic properties. The methanolic extract was assessed for its in vivo anti-diabetic effects. The results indicated the presence of alkaloid, protein and amino acids, carbohydrates, glycosides, flavonoids, and saponins along with the highest brine shrimp lethality (90.7 ± 1.8%), α-amylase inhibition (60.2%) and free radical scavenging properties. The diabetic control indicated a significant (p<0.05) decrease in weight and high-density lipoprotein and elevated level of blood glucose, bilirubin, creatinine, urea, triglyceride, low density lipoprotein, very low-density lipoprotein, transaminases, and alkaline phosphatase. All these changes were restored significantly (p<0.05) by treatment of diabetic rats with methanolic extract of I. aucheri. The results show the potential of I. aucheri as a source of therapeutic compounds against diabetic, hyperlipidemia and free radical associated disorders.
{"title":"Anti-diabetic activity of Iphiona aucheri leaf extract","authors":"Muhammad Abdur Rehman Shah, R. Khan, Mushtaq Ahmed","doi":"10.3329/BJP.V15I4.44170","DOIUrl":"https://doi.org/10.3329/BJP.V15I4.44170","url":null,"abstract":"The methanolic extract of Iphiona aucheri leaves and its n-hexane, chloroform and aqueous fractions were screened for phytochemicals, cytotoxic effects, total phenolic contents, antioxidant and anti-diabetic properties. The methanolic extract was assessed for its in vivo anti-diabetic effects. The results indicated the presence of alkaloid, protein and amino acids, carbohydrates, glycosides, flavonoids, and saponins along with the highest brine shrimp lethality (90.7 ± 1.8%), α-amylase inhibition (60.2%) and free radical scavenging properties. The diabetic control indicated a significant (p<0.05) decrease in weight and high-density lipoprotein and elevated level of blood glucose, bilirubin, creatinine, urea, triglyceride, low density lipoprotein, very low-density lipoprotein, transaminases, and alkaline phosphatase. All these changes were restored significantly (p<0.05) by treatment of diabetic rats with methanolic extract of I. aucheri. The results show the potential of I. aucheri as a source of therapeutic compounds against diabetic, hyperlipidemia and free radical associated disorders.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJP.V15I4.44170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47887233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lipid lowering efficacy of fraxetin, a coumarin derivative on high fat diet induced hypercholesterolemic rats","authors":"A. Purushothaman, R. Sundaram","doi":"10.3329/bjp.v15i3.47113","DOIUrl":"https://doi.org/10.3329/bjp.v15i3.47113","url":null,"abstract":"","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2020-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47729415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nouari Sadrati, A. Zerroug, Rasime Demirel, Sabrina Bakli, D. Harzallah
The in vitro antibacterial activity of the endophytic fungus Aspergillus neobridgeri PLR9 (isolated from the plant Pistacia lentiscus) against a wide range of tested bacteria were studied. The maximum inhibitory activity was found on malt extract agar medium with a mean inhibitory zone of 11.3 mm (p<0.05). The ethyl acetate extract showed a significant inhibitory zone of 13.4 mm (p<0.05), followed by dichloromethane 9.1 mm. Whereas, n-hexane extract showed no activity. The largest inhibition zones were observed against Staphylococcus aureus 3, S. aureus 5 and Escherichia coli 1 with 21.0 mm of diameter. The MIC's were ranged between 312.5 and 1,250 μg/mL. The MBC's were ≤2500 μg/mL for most Gram positive bacteria and ≥2500 μg/mL for most Gram negative bacteria. Time kill assay study against the Enterococcus faecium showed a bactericidal effect at 4x MIC and bacteriostatic against Acinetobacter baumannii at different concentrations. Moreover, it is the first report, PLR9 isolated as endophyte and studied for their antimicrobial activity.
{"title":"Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria","authors":"Nouari Sadrati, A. Zerroug, Rasime Demirel, Sabrina Bakli, D. Harzallah","doi":"10.3329/bjp.v15i3.40923","DOIUrl":"https://doi.org/10.3329/bjp.v15i3.40923","url":null,"abstract":"The in vitro antibacterial activity of the endophytic fungus Aspergillus neobridgeri PLR9 (isolated from the plant Pistacia lentiscus) against a wide range of tested bacteria were studied. The maximum inhibitory activity was found on malt extract agar medium with a mean inhibitory zone of 11.3 mm (p<0.05). The ethyl acetate extract showed a significant inhibitory zone of 13.4 mm (p<0.05), followed by dichloromethane 9.1 mm. Whereas, n-hexane extract showed no activity. The largest inhibition zones were observed against Staphylococcus aureus 3, S. aureus 5 and Escherichia coli 1 with 21.0 mm of diameter. The MIC's were ranged between 312.5 and 1,250 μg/mL. The MBC's were ≤2500 μg/mL for most Gram positive bacteria and ≥2500 μg/mL for most Gram negative bacteria. Time kill assay study against the Enterococcus faecium showed a bactericidal effect at 4x MIC and bacteriostatic against Acinetobacter baumannii at different concentrations. Moreover, it is the first report, PLR9 isolated as endophyte and studied for their antimicrobial activity.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2020-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49535764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study explores the hepatoprotective and in vivo antioxidant activities of Misopates orontium. Toxicity studies were conducted on human red blood cells and DNA. Initially, acute hepatoprotective study was done on Wistar rats by giving crude, aqueous, n-hexane, chloroform, ethyl acetate and n-butanol fractions 200 mg/kg/day for 24 hours. Liver function tests were performed. The ethyl acetate fraction was selected for 14 days study. Hepatotoxicity induced by paracetamol (350 mg/kg) orally. All the liver function tests, liver peroxidation tests i.e malondialdehyde, glutathione and histopathological study of the liver was performed at the end of study. In hepatoprotective studies, ethyl acetate fraction showed a significant decrease in the level of serum enzymes (97.6 IU/L), (101 IU/L), (145.17 IU/L) and (0.68 mg/dL). Peroxidation levels in liver homogenates of ethyl acetate-treated group decreased in malondialdehyde (42.5) while reduced glutathione level increased (106.5 nmol/g). This study shows that M. orontium has hepatoprotective and antioxidant effects.
{"title":"Hepatoprotective effect of Misopates orontium in rats","authors":"S. Akbar, Saiqa Ishtiaq","doi":"10.3329/bjp.v15i3.45334","DOIUrl":"https://doi.org/10.3329/bjp.v15i3.45334","url":null,"abstract":"This study explores the hepatoprotective and in vivo antioxidant activities of Misopates orontium. Toxicity studies were conducted on human red blood cells and DNA. Initially, acute hepatoprotective study was done on Wistar rats by giving crude, aqueous, n-hexane, chloroform, ethyl acetate and n-butanol fractions 200 mg/kg/day for 24 hours. Liver function tests were performed. The ethyl acetate fraction was selected for 14 days study. Hepatotoxicity induced by paracetamol (350 mg/kg) orally. All the liver function tests, liver peroxidation tests i.e malondialdehyde, glutathione and histopathological study of the liver was performed at the end of study. In hepatoprotective studies, ethyl acetate fraction showed a significant decrease in the level of serum enzymes (97.6 IU/L), (101 IU/L), (145.17 IU/L) and (0.68 mg/dL). Peroxidation levels in liver homogenates of ethyl acetate-treated group decreased in malondialdehyde (42.5) while reduced glutathione level increased (106.5 nmol/g). This study shows that M. orontium has hepatoprotective and antioxidant effects.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2020-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i3.45334","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47144755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anti-inflammatory and antifungal activity of Dracaena victoria leaf extract","authors":"R. Sundar, Sathiavelu Arunachalam","doi":"10.3329/bjp.v15i1.44399","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.44399","url":null,"abstract":"","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"15 1","pages":"44-45"},"PeriodicalIF":1.6,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i1.44399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44535687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The term, ischemia, refers to a condition which is characterized by enormous reduction of glucose and oxygen. In cerebral ischemia, the cerebral blood flow become insufficient in order to pursue cerebral meta-bolic activities (Ahmed at al., 2018). Both, the inflammation and oxidative stress have a promising role in cerebral infarction associated with ischemic reperfusion insult. The reperfusion insult results in an initiation of a number of events including stress oxida-tive, devastation of blood brain barrier, leukocytes intrusion, inflammation, apoptosis and nitric oxide discharge, etc. In this context, potential antioxidant and anti-inflammatory intermediaries might play their role to lessen the effects produced as a result of ischemic reperfusion insult. Due to lack of active treatment of ischemic reperfusion associated cerebral ischemia, researchers interest in developing some effective therapy from medicinal plants is obvious (Kalogeris et al., 2012). In order to get to know about the mechanism of cell demise and neuroprotection, a classical rat’s model is widely used. In this model, the forebrain slices of the animal are bare to oxygen and glucose deficiency and reperfusion (Ca´rdenas et al., 2000). The advantages of this model are time tested as cell composition is preserved.
{"title":"Effect of Garcinia indica against brain ischemic reperfusion in rat model","authors":"W. Ahmed","doi":"10.3329/bjp.v15i1.43150","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.43150","url":null,"abstract":"The term, ischemia, refers to a condition which is characterized by enormous reduction of glucose and oxygen. In cerebral ischemia, the cerebral blood flow become insufficient in order to pursue cerebral meta-bolic activities (Ahmed at al., 2018). Both, the inflammation and oxidative stress have a promising role in cerebral infarction associated with ischemic reperfusion insult. The reperfusion insult results in an initiation of a number of events including stress oxida-tive, devastation of blood brain barrier, leukocytes intrusion, inflammation, apoptosis and nitric oxide discharge, etc. In this context, potential antioxidant and anti-inflammatory intermediaries might play their role to lessen the effects produced as a result of ischemic reperfusion insult. Due to lack of active treatment of ischemic reperfusion associated cerebral ischemia, researchers interest in developing some effective therapy from medicinal plants is obvious (Kalogeris et al., 2012). In order to get to know about the mechanism of cell demise and neuroprotection, a classical rat’s model is widely used. In this model, the forebrain slices of the animal are bare to oxygen and glucose deficiency and reperfusion (Ca´rdenas et al., 2000). The advantages of this model are time tested as cell composition is preserved.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"15 1","pages":"39-40"},"PeriodicalIF":1.6,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i1.43150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42288865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study of antimutagenic activity gives information which discovers the possibilities for combating the genotoxic risk of mutagens. Genotoxicity includes DNA damage, gene mutation, chromosomal aberration, and formation of micronucleus (Swift, Golsteyn, 2014). Genotoxicity commonly occurs with anti-cancer drugs which causes a high level of DNA damage that activa-tes cell cycle checkpoints leading to cell cycle arrest or cell death (Helleday et al., 2008). Several studies on medicinal plants have revealed antimutagenic and antigenotoxic properties mainly due to antioxidants that scavenge reactive oxygen species (ROS) (Zani et al., 1993; Gonzalez-Avila et al., 2003; Park et al., 2004).
{"title":"In vitro antimutagenic activity of Ipomoea staphylina","authors":"A. Banerjee, S. Mumtaz","doi":"10.3329/bjp.v15i1.43446","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.43446","url":null,"abstract":"The study of antimutagenic activity gives information which discovers the possibilities for combating the genotoxic risk of mutagens. Genotoxicity includes DNA damage, gene mutation, chromosomal aberration, and formation of micronucleus (Swift, Golsteyn, 2014). Genotoxicity commonly occurs with anti-cancer drugs which causes a high level of DNA damage that activa-tes cell cycle checkpoints leading to cell cycle arrest or cell death (Helleday et al., 2008). Several studies on medicinal plants have revealed antimutagenic and antigenotoxic properties mainly due to antioxidants that scavenge reactive oxygen species (ROS) (Zani et al., 1993; Gonzalez-Avila et al., 2003; Park et al., 2004).","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"15 1","pages":"41-43"},"PeriodicalIF":1.6,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42662855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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