The present study aimed to explore the effects of α-cyperone, an extract of Cyperus rotundus, on PC12 cells, as well as the underlying mechanism. Following the cells were induced by oxygen-glucose deprivation/reoxygena-tion (OGD/R), the viability, morphology, inflammation, oxidative stress and apoptotic levels in the cells were evaluated. To explore the mechanism of α-cyperone, cells were treated with 3-TYP, a sirtuin-3 (SIRT3) inhibitor, and then the effects of 3-TYP on the function of α-cyperone were assessed. α-Cyperone was found to reduce OGD/R-induced damage to neuronal viability and alleviate inflammation, oxidative stress, and apoptosis. In addition, α-cyperone could elevate SIRT3 and decline acetyl-forkhead box O1 (FOXO1) levels, and 3-TYP broke the effects of α-cyperone on the aforementioned aspects in the PC12 cells. In conclusion, α-cyperone activated SIRT3 and FOXO1 deacetylation, and alleviated OGD/R-induced cell inflammation, oxidative stress, and apoptosis.
{"title":"Sesquiterpene compound α-cyperone relieves the injury in neurons undergoing oxygen-glucose deprivation/reoxygenation","authors":"Jian Wang, Xuefeng Gao","doi":"10.3329/bjp.v17i2.58188","DOIUrl":"https://doi.org/10.3329/bjp.v17i2.58188","url":null,"abstract":"The present study aimed to explore the effects of α-cyperone, an extract of Cyperus rotundus, on PC12 cells, as well as the underlying mechanism. Following the cells were induced by oxygen-glucose deprivation/reoxygena-tion (OGD/R), the viability, morphology, inflammation, oxidative stress and apoptotic levels in the cells were evaluated. To explore the mechanism of α-cyperone, cells were treated with 3-TYP, a sirtuin-3 (SIRT3) inhibitor, and then the effects of 3-TYP on the function of α-cyperone were assessed. α-Cyperone was found to reduce OGD/R-induced damage to neuronal viability and alleviate inflammation, oxidative stress, and apoptosis. In addition, α-cyperone could elevate SIRT3 and decline acetyl-forkhead box O1 (FOXO1) levels, and 3-TYP broke the effects of α-cyperone on the aforementioned aspects in the PC12 cells. In conclusion, α-cyperone activated SIRT3 and FOXO1 deacetylation, and alleviated OGD/R-induced cell inflammation, oxidative stress, and apoptosis.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43048454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Majda Badaoui, S. Moubtakir, Chafik Terrafe, R. Aboufatima, A. Chait
This study aims to examine the antianxiety and antidepressant effects of aqueous latex extract of Euphorbia resinifera in mice. Antianxiety and sedative effects were examined using the elevated plus maze test, open field test, and thiopental-induced sleeping time respectively. While the antidepressant effect was evaluated, using the forced swimming test. E. resinifera reduced the latency of sleeping and increased sleeping time significantly at 75 mg/kg. It reduced the immobility time and increased swimming significantly at all doses assessed (25, 50, and 75 mg/kg). Pretreatment with antagonists reversed these effects indicating the possible involvement of α2, 5HT2, D2, and GABAA receptors respectively. These findings confirm the traditional utilization of this plant as an antioxidant, anxiolytic, and antidepressant.
{"title":"Antianxiety and antidepressant effects of aqueous latex extract of Euphorbia resinifera","authors":"Majda Badaoui, S. Moubtakir, Chafik Terrafe, R. Aboufatima, A. Chait","doi":"10.3329/bjp.v17i2.59258","DOIUrl":"https://doi.org/10.3329/bjp.v17i2.59258","url":null,"abstract":"This study aims to examine the antianxiety and antidepressant effects of aqueous latex extract of Euphorbia resinifera in mice. Antianxiety and sedative effects were examined using the elevated plus maze test, open field test, and thiopental-induced sleeping time respectively. While the antidepressant effect was evaluated, using the forced swimming test. E. resinifera reduced the latency of sleeping and increased sleeping time significantly at 75 mg/kg. It reduced the immobility time and increased swimming significantly at all doses assessed (25, 50, and 75 mg/kg). Pretreatment with antagonists reversed these effects indicating the possible involvement of α2, 5HT2, D2, and GABAA receptors respectively. These findings confirm the traditional utilization of this plant as an antioxidant, anxiolytic, and antidepressant.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48492714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. F. D. Gatphoh, Natasha Naval Aggarwal, S. M. Kumar, M. Kumar, B. Revanasiddappa
The compounds 1,3,4-oxadiazole derivatives (1-8) were synthesized by the cyclization of 4-hydroxy benzhydrazide (1) with various substituted aroma-tic aldehydes (2) using FeCl3 as catalyst and methanol as a solvent medium. The structures of the newly synthesized compounds were assigned based on FT-IR, 1H-NMR, and mass spectral data. In vivo antidepressant activity was performed by tail suspension test and forced swimming test models. Using the Schrodinger Maestro, the in silico analysis was performed and docked to the glycogen synthase kinase 3β binding site (PDB: 3GB2). Compounds 8 [4,4'-(1,3,4-oxadiazole-2,5-diyl)diphenol] and 3 [3-(5-(4-hydroxyphenyl)-1,3,4-oxadiazol-2-yl) phenol] showed both potent inhibitory activity against GSK-3β with a docking score of -7.800 kcal/mol as well as good antidepressant activity in both tail suspension and forced swimming tests models. The synthesized derivatives showed good antidepressive potential.
{"title":"Synthesis, in silico analysis and antidepressant activity of 1,3,4-oxadiazole derivatives","authors":"B. F. D. Gatphoh, Natasha Naval Aggarwal, S. M. Kumar, M. Kumar, B. Revanasiddappa","doi":"10.3329/bjp.v17i1.58728","DOIUrl":"https://doi.org/10.3329/bjp.v17i1.58728","url":null,"abstract":"The compounds 1,3,4-oxadiazole derivatives (1-8) were synthesized by the cyclization of 4-hydroxy benzhydrazide (1) with various substituted aroma-tic aldehydes (2) using FeCl3 as catalyst and methanol as a solvent medium. The structures of the newly synthesized compounds were assigned based on FT-IR, 1H-NMR, and mass spectral data. In vivo antidepressant activity was performed by tail suspension test and forced swimming test models. Using the Schrodinger Maestro, the in silico analysis was performed and docked to the glycogen synthase kinase 3β binding site (PDB: 3GB2). Compounds 8 [4,4'-(1,3,4-oxadiazole-2,5-diyl)diphenol] and 3 [3-(5-(4-hydroxyphenyl)-1,3,4-oxadiazol-2-yl) phenol] showed both potent inhibitory activity against GSK-3β with a docking score of -7.800 kcal/mol as well as good antidepressant activity in both tail suspension and forced swimming tests models. The synthesized derivatives showed good antidepressive potential.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49534998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cost-effectiveness analysis of intradermal versus classical intramuscular COVID-19 vaccine administration","authors":"P. Sookaromdee, V. Wiwanitkit","doi":"10.3329/bjp.v17i1.57593","DOIUrl":"https://doi.org/10.3329/bjp.v17i1.57593","url":null,"abstract":"<jats:p>n/a</jats:p>","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45933209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cicadidae Periostracum has various pharmacological effects, including neuroprotective potential via nuclear receptor related-1 (NURR1) protein signaling. However, there are no studies on its antioxidative effect. The neuroprotective effect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced damage to dopaminergic neurons was evaluated and explored the mechanisms of its antioxidant action, focusing on nuclear factor E2-related factor 2 (Nrf2) in a mouse model of Parkinson's disease. We measured NURR1-related neurotrophic signaling and the levels of antioxidant factors in vitro and in vivo. The effects of Cicadidae Periostracum against MPTP-induced neurotoxicity were associated with inhibition of the neurotrophic signaling pathways and up-regulation of Nrf2 pathways. Thus, Cicadidae Periostracum mitigates neurotoxicity via neurotrophic signaling not only by increasing Nrf2 activity but also by increasing antioxidant activity.
{"title":"Nrf2 signaling contributes to the neuroprotective effect of Cicadidae Periostracum against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine toxicity","authors":"Hye-Sun Lim, B. Moon, G. Park","doi":"10.3329/bjp.v17i1.57472","DOIUrl":"https://doi.org/10.3329/bjp.v17i1.57472","url":null,"abstract":"Cicadidae Periostracum has various pharmacological effects, including neuroprotective potential via nuclear receptor related-1 (NURR1) protein signaling. However, there are no studies on its antioxidative effect. The neuroprotective effect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced damage to dopaminergic neurons was evaluated and explored the mechanisms of its antioxidant action, focusing on nuclear factor E2-related factor 2 (Nrf2) in a mouse model of Parkinson's disease. We measured NURR1-related neurotrophic signaling and the levels of antioxidant factors in vitro and in vivo. The effects of Cicadidae Periostracum against MPTP-induced neurotoxicity were associated with inhibition of the neurotrophic signaling pathways and up-regulation of Nrf2 pathways. Thus, Cicadidae Periostracum mitigates neurotoxicity via neurotrophic signaling not only by increasing Nrf2 activity but also by increasing antioxidant activity.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49146677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study has investigated how mulberrofuran G affects prolifera-tion, invasion, and migration of the lung adenocarcinoma and squamous cell carcinoma. Four different concentrations of mulberrofuran G (1, 5, 10, and 100 μmol/L) were used to simulate human lung adenocarcinoma cells (A549 cells) and squamous-cell carcinoma (NCI-H226 cells). The results showed that mulberrofuran G significantly inhibited the proliferation, invasion, and migration of both A549 and NCI-H226 cells, with a dose-effect relationship. The IC50 inhibited the growth of A549 cells and NCI-H226 cells by mulberrofuran G were 22.5 and 30.6 μmol/L, respectively. It strengthened the expression of CDK4 and MMP9 but significantly weakened the expression of p27 in both A549 cells and NCI-H226 cells. Furthermore, the expression of p-JAK2 and p-STAT3 was significantly down-regulated in drug treatments. In conclusion, mulberrofuran G could inhibit proliferation, migration, and invasion of lung cancer cells via inactivating JAK2/STAT3 signaling.
{"title":"Mulberrofuran G inhibits proliferation and migration by inactivating JAK2/STAT3 signaling in lung cancer cells","authors":"Haifeng Guo, Xiao-xiao Liu, X. Zhu, Zhe Yu","doi":"10.3329/bjp.v16i4.55198","DOIUrl":"https://doi.org/10.3329/bjp.v16i4.55198","url":null,"abstract":"The present study has investigated how mulberrofuran G affects prolifera-tion, invasion, and migration of the lung adenocarcinoma and squamous cell carcinoma. Four different concentrations of mulberrofuran G (1, 5, 10, and 100 μmol/L) were used to simulate human lung adenocarcinoma cells (A549 cells) and squamous-cell carcinoma (NCI-H226 cells). The results showed that mulberrofuran G significantly inhibited the proliferation, invasion, and migration of both A549 and NCI-H226 cells, with a dose-effect relationship. The IC50 inhibited the growth of A549 cells and NCI-H226 cells by mulberrofuran G were 22.5 and 30.6 μmol/L, respectively. It strengthened the expression of CDK4 and MMP9 but significantly weakened the expression of p27 in both A549 cells and NCI-H226 cells. Furthermore, the expression of p-JAK2 and p-STAT3 was significantly down-regulated in drug treatments. In conclusion, mulberrofuran G could inhibit proliferation, migration, and invasion of lung cancer cells via inactivating JAK2/STAT3 signaling.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2021-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42608369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pharmacological activities and volatile organic compounds from the endophytic bacteria from the leaves of Rhizophora apiculata","authors":"T. T. H. Dat, P. T. Oanh","doi":"10.3329/bjp.v16i4.56682","DOIUrl":"https://doi.org/10.3329/bjp.v16i4.56682","url":null,"abstract":"","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46175465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anti-cancer property of fungi derived β-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of β-glucan (62.5, 125, 250 and 500 μg/mL) and the viability of the cells was tested using the XTT assay after 24 hours. Cleaved PARP, BCL-2, 8-hydroxy-desoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant levels in the cells were measured by commercial kits. β-Glucan significantly decreased the cell viability in SH-SY5Y cells. ELISA tests demonstrated that β-glucan therapy dramatically increased 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP, total oxidant. However, β-glucan treatment did not change the BCL-2 protein level. Altogether, β-glucan caused significant cytotoxicity in SH-SY5Y cells by inducing oxidative stress, increasing DNA damage, and ultimately increasing apoptosis.
{"title":"Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line","authors":"A. Filiz, Ziad Joha, Fatih Yulak","doi":"10.3329/bjp.v16i4.54872","DOIUrl":"https://doi.org/10.3329/bjp.v16i4.54872","url":null,"abstract":"Anti-cancer property of fungi derived β-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of β-glucan (62.5, 125, 250 and 500 μg/mL) and the viability of the cells was tested using the XTT assay after 24 hours. Cleaved PARP, BCL-2, 8-hydroxy-desoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant levels in the cells were measured by commercial kits. β-Glucan significantly decreased the cell viability in SH-SY5Y cells. ELISA tests demonstrated that β-glucan therapy dramatically increased 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP, total oxidant. However, β-glucan treatment did not change the BCL-2 protein level. Altogether, β-glucan caused significant cytotoxicity in SH-SY5Y cells by inducing oxidative stress, increasing DNA damage, and ultimately increasing apoptosis.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44160510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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